Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 12 de 12
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Front Med (Lausanne) ; 8: 712222, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34485341

RESUMO

Objective: This study aims to investigate the clinical efficacy of laparoscopy and hysteroscopy in the treatment of tubal-factor infertility (TFI) to provide a basis for predicting postoperative pregnancy rates. Methods: The clinical data of 336 patients who underwent laparoscopy and hysteroscopy for TFI between February 2018 and December 2018 in the Department of Reproductive Gynecology at the First People's Hospital of Yunnan were retrospectively analyzed. After implementing the inclusion and exclusion criteria, 278 patients were included in the study. The patients were grouped according to pelvic adhesions, hydrosalpinx, twisted fallopian tubes, and fimbriae structure. The impact of the extent of fallopian tube diseases on postoperative pregnancy outcomes was analyzed. Results: Of the 278 patients, 129 got pregnant (pregnancy rate = 46.4%). Pelvic adhesions, hydrosalpinx, twisted/folded fallopian tubes, and damage to the fimbriae of the fallopian tubes were found to affect the natural pregnancy rate after surgery, and it decreased significantly with the aggravation of the disease (P < 0.001). Of the 129 patients who had natural pregnancies, 29 had ectopic pregnancies (ectopic pregnancy rate = 22.48%). Twisted/folded fallopian tubes and damage to the fimbriae structure significantly increased the incidence of postoperative ectopic pregnancy (P < 0.001). Conclusion: Laparoscopy and hysteroscopy are effective treatments for TFI. Pelvic adhesions, twisted/folded fallopian tubes, hydrosalpinx, and damage to the fimbriae of the fallopian tubes can affect postoperative pregnancy outcomes and lead to failure of a natural pregnancy after the operation. The incidence of ectopic pregnancy increases with the degree of fallopian tube twisting/folding and the degree of damage to the fimbriae of the fallopian tubes.

2.
Environ Sci Pollut Res Int ; 28(36): 49880-49888, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33948833

RESUMO

A new strategy for the wastewater treatment was proposed by combining polyvinylpyrrolidone-functionalized silver nanoparticles with reduced graphene oxide (AgNPs-PVP@rGO) as a visible light-triggered photoactive nanocomposite. The nanocomposite with enhanced photocatalytic degradation and photothermal antibacterial activity can simultaneously decrease the content of organic pollutants and bacteria in the wastewater under visible light irradiation. The efficiency of photocatalytic degradation can be significantly improved by the conjugation of AgNPs onto the rGO surface. The water solubility and dispersion of nanocomposite can be increased via PVP functionalization, without stirring during the photocatalytic process. Under the optimal synthesis condition, AgNPs-PVP@rGO has a photocatalytic degradation efficiency of 90.1% for rhodamine B, which is 6.9 and 1.8 times higher than that of polyvinylpyrrolidone-functionalized silver nanoparticles and rGO alone, respectively. More importantly, the degradation efficiency of optimal AgNPs-PVP@rGO sol on rhodamine B is significantly higher than that of its block suspension in the same amount, indicating that the sol with more specific surface area is conducive to the photocatalytic reaction. Meanwhile, the AgNPs-PVP@rGO with excellent photothermal activity can effectively inhibit the bacterial growth. This functional modification of graphene provides a new strategy for simultaneous treatment of multiple pollutants in wastewater. The AgNPs-PVP@rGO nanocomposites for simultaneous enhanced photocatalytic degradation and photothermal antibacterial activity by visible light.


Assuntos
Grafite , Nanopartículas Metálicas , Nanocompostos , Antibacterianos/farmacologia , Luz , Prata
3.
Insect Sci ; 27(4): 646-654, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31131541

RESUMO

MicroRNAs (miRNAs) are a class of short, non-coding transcripts that bind to 3'-untranslated regions to trigger messenger RNA degradation or translational inhibition. Here we explored how miRNAs regulate sex determination in Bombyx mori, a lepidopteran model insect. Genes known to be involved in sex determination, BmPSI, Bmdsx, and BmMasc, are predicted targets of the species-specific miR-2738. Using a dual luciferase reporter assay in HEK293T cells, we confirmed that miR-2738 suppressed transcription of BmPSI, Bmdsx, and BmMasc. The levels of BmPSI and BmMasc were significantly down-regulated in B. mori miR-2738 overexpression. In contrast, the genetic disruption of miR-2738 using the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 transgenic system increased the levels of BmPSI and BmMasc transcripts, whereas splicing of Bmdsx was unaltered by miR-2738 depletion or overexpression. Taken together, this study implicates miR-2738 as a minor regulator of sex determination genes in the silkworm.


Assuntos
Bombyx/crescimento & desenvolvimento , Bombyx/genética , Regulação da Expressão Gênica , MicroRNAs/genética , Processos de Determinação Sexual/genética , Animais , Animais Geneticamente Modificados/genética , Animais Geneticamente Modificados/crescimento & desenvolvimento , Animais Geneticamente Modificados/metabolismo , Sequência de Bases , Bombyx/metabolismo , Células HEK293 , Humanos , MicroRNAs/metabolismo
4.
Insect Sci ; 26(6): 991-999, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30549429

RESUMO

Identification of stage- and tissue-specific cis-regulatory elements will enable more precise genomic editing. In previous studies of the silkworm Bombyx mori, we identified and characterized several tissue- and sex-specific cis-regulatory elements using transgenic technology, including a female- and fat body-specific promoter, vitellogenin, testis-specific promoters, Radial spoke head 1 (BmR1) and beta-tubulin 4 (Bmß4). Here we report a cis-regulatory element specific for a somatic and germ cell-expressed promoter, nanos (Bmnos). We investigated activities of three truncated promoter sequences upstream of the transcriptional initiation site sequences of Bmnos in vitro (nos-0.6kb, nos-1kb and nos-2kb) and in vivo (nos-2kb). In BmN cultured cells, all three lengths drove expression of the gene encoding enhanced green fluorescence protein (EGFP), although nos-2kb had the highest fluorescence activity. In transgenic silkworms, nos-2kb drove EGFP expression at the early embryonic stage, and fluorescence was concentrated in the gonads at later embryonic stages. In addition, this cis-regulatory element was not sex differentiated. The fluorescence intensity gradually weakened following the larval developmental stage in the gonads and were broadly expressed in the whole body. The nos-2kb promoter drove the Cas9 system with efficiency comparable to that of the broad-spectrum strong IE1 promoter. These results indicate that Bmnos is an effective endogenous cis-regulatory element in the early embryo and in the gonad that can be used in applications involving the clustered, regularly interspaced, short palindromic repeats (CRISPR)/Cas9 system.


Assuntos
Bombyx/genética , Edição de Genes , Regiões Promotoras Genéticas , Animais , Animais Geneticamente Modificados , Sistemas CRISPR-Cas , Linhagem Celular , Embrião não Mamífero , Genes Reporter , Proteínas de Fluorescência Verde , Transformação Genética
5.
Nan Fang Yi Ke Da Xue Xue Bao ; 37(9): 1143-1148, 2017 Sep 20.
Artigo em Chinês | MEDLINE | ID: mdl-28951353

RESUMO

OBJECTIVE: To assess the impact of delayed decompression on long-term neurological and bladder function recovery in patients with cauda equina syndrome (CES) secondary to lumbar disc herniation (LDH). METHODS: The clinical data of 35 patients receiving delayed decompression surgery for CES secondary to LDH were reviewed. The bladder empty function, bowel control, sexual ability and neurological functions of the lower limbs were evaluated after the operation, and the urodynamic changes were assessed in 6 patients with urodynamic data before and after the operation. RESULTS: Surgical decompression was performed at 4.1∓3.9 weeks in 12 patients with complete CES and at 5.5∓7.6 weeks in 23 patients with incomplete CES after the onset of symptoms. The patients were followed up for a mean of 43.0∓28.9 months (3-110 months). In the 23 patients with incomplete CES, 19 obtained full recovery, 4 had slight sensory alterations in the saddle area or the lower limbs. In the 12 patients with complete CES, 2 had full recovery, 4 reported slight sensory alterations in the saddle area or the lower limbs (including 2 with occasional constipation); 6 still had sense deficit in the saddle area and difficulties in bladder or bowl emptying, but they all reported significant improvements compared to the condition before operation. Urodynamic analysis in the 6 patients with pre- and postoperative urodynamic data showed increased abdominal pressure when voiding with significantly reduced residual urine in all the 6 patients; 4 patients with abnormal first desire volume before operation reported recovery after the operation. CONCLUSION: Patients with LDH-induced CES who missed the chance of early decompression can still expect favorable functional recovery in the long term. The improvement of bladder function following decompression is probably a result of recovery of bladder sensation and the compensation by increased intra-abdominal pressure. The key strategy to promote bladder function recovery in these patients is to promote the detrusor recovery.

6.
Nan Fang Yi Ke Da Xue Xue Bao ; 36(6): 785-9, 2016 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-27320879

RESUMO

OBJECTIVE: To investigate the effect of the non-PLC-dependent protein kinase C (PKC) pathway of parathyroid hormone (PTH) on the apoptosis and proliferation of osteoblast MC-3T3E1 cells. METHODS: MC-3T3E1 cells were seeded in 96-well plates at the density of 1.5×10(4) cells/mL and incubated for 3 day. The cells were then exposed to 100 nmol/L of [Gly(1), Arg(19)]hPTH(1-28), 100 nmol/L of [Gly(1), Arg(19)]hPTH(1-34), 100 nmol/L of [Gly(1), Arg(19)]hPTH(1-34)+1 µmol/L Go6983, 1 µmol/L Go6983, or deionized water (control) for 1, 24 or 48 h. After the treatments, cell counting kit-8 (CCK-8) and Caspase-Glo® 3/7 Assay (Caspase-3) were used to examine the proliferation and apoptosis of MC3T3-E1 cells. RESULTS: CCK-8 results showed that hPTH(1-34) increased the number of MC3T3-E1 cells compared with hPTH(1-34)+Go6983 at 1 h and 24 h, but this difference was not statistically different. At 48 h, treatment with hPTH(1-34), as compared with hPTH(1-28), significantly increased the number of MC3T3-E1 cells (P<0.05), and this effect was blocked by the PKC inhibitor Go6983 (P<0.05). hPTH(1-34) did not result in significant inhibition of MC3T3-E1 cell apoptosis at 1 h and 24 h as compared with hPTH(1-34)+Go6983, but significantly inhibited the cell apoptosis as compared with hPTH(1-28) (P<0.05); this inhibitory effect was blocked by Go6983 (P<0.05). CONCLUSION: s A relatively long time (for 48 h) of exposure to PTH can inhibit apoptosis and promote the proliferation of MC3T3-E1cells through a non-PLC-dependent PKC pathway.


Assuntos
Apoptose , Hormônio Paratireóideo/farmacologia , Proteína Quinase C/metabolismo , Transdução de Sinais , Células 3T3 , Animais , Proliferação de Células , Indóis/farmacologia , Maleimidas/farmacologia , Camundongos , Osteoblastos , Proteína Quinase C/antagonistas & inibidores
7.
Nan Fang Yi Ke Da Xue Xue Bao ; 36(3): 414-8, 2016 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-27063174

RESUMO

OBJECTIVE: To evaluate the efficacy of conservative treatment with teriparatide for promoting bone fracture healing in patients with osteoporotic vertebral fracture. METHODS: Twelve postmenopausal patients (aged 73±4.8 years) with osteoporotic spinal fracture confirmed by MRI or CT scanning received conservative treatment with teriparatidesc injection supplemented with calcium and analgesics for 6 months. At the beginning and at the end of the therapy, VAS score, Oswestry Disability Index (ODI), bone mass densitometry, and X-ray of the thoracic and lumbar spine, and serum P1NP and beta-CTX levels were measured. Six of the patients received a second MRI scan after the therapy to evaluate the bone healing. RESULTS: All the 12 patients completed the treatment, during which no new fractures or adverse events occurred. At the end of the first month of treatment, analgesic was withdrawn for all the patients. The average VAS score decreased from 8±2 to 1±2 at 1 month during the therapy, and ODI was reduced from (76±12)% to (20±5)% at 1 month and further to (5±4)% at 6 month. After the 6-month therapy, the height of the fractured vertebrae (presented as the anterior to posterior wall height ratio) was insignificantly decreased from (75±20)% to (61±20)%, the BMD was increased by (20±5)%, P1NP increased significantly from 20.9±11.4 ng/mL to 80.0±41.2 ng/mL, and beta-CTX increased from 0.30±0.17 ng/mL to 0.51±0.3 ng/mL. The 6 patients re-examined with MRI demonstrated complete bone healing after the therapy. CONCLUSION: Teriparatide is effective for conservative treatment of osteoporotic spinal fracture and can promote bone fracture healing, improve the quality of life, and prevents vertebral collapse, and can be therefore an alternative treatment to PVP or BV.


Assuntos
Fraturas por Compressão/tratamento farmacológico , Fraturas por Osteoporose/tratamento farmacológico , Fraturas da Coluna Vertebral/tratamento farmacológico , Teriparatida/uso terapêutico , Idoso , Analgésicos/uso terapêutico , Densidade Óssea , Cálcio/uso terapêutico , Humanos , Vértebras Lombares/patologia , Imageamento por Ressonância Magnética , Medição da Dor , Qualidade de Vida , Resultado do Tratamento
8.
Nan Fang Yi Ke Da Xue Xue Bao ; 35(4): 486-91, 2015 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-25907930

RESUMO

OBJECTIVE: To explore the functions of phospholipase C (PLC)-independent protein kinase C signaling pathway (PTH/nonPLC/PKC) of parathyroid hormone (PTH) and its role in bone metabolism. METHODS: Osteoblasts isolated from the calvaria of 2- or 3-day-old C57BL mice, identified by alkaline phosphatase staining and Alizarin red staining, were treated for 4 h with 100 nmol/L [Gly(1), Arg(19)]hPTH(1-28) plus 10 nmol/L RP-cAMP, 10 nmol/L [Gly(1), Arg(19)]hPTH(1-34) plus 10 nmol/L RP-cAMP , 10 nmol/L PTH(1-34), or and 0.1% trifluoroacetic acid (TFA). The total RNA was then isolated for screening differentially expressed genes related to PTH/nonPLC/PKC pathway using Affymetrix mouse 12x135K gene expression profile microarray, and the identified genes were confirmed by real-time quantitative PCR. MC3T3-E1 cells treated with [Gly(1), Arg(19)]hPTH(1-28)+RP-cAMP, [Gly(1), Arg(19)]hPTH(1-34)+RP-cAMP, [Gly(1), Arg(19)]hPTH(1-34)+ RP-cAMP +100 nmol/L Go6983, or 0.1% TFA were also examined for GR(1-28)- or GR(1-34)-mediated gene expression changes using real-time quantitative PCR. RESULTS: Alizarin red staining visualized red mineralized nodules in the osteoblasts at 28 days of culture. According to the genechip results, we selected 56 target genes related to PTH/nonPLC/PKC pathway, among which CITED1 showed higher expressions in [Gly(1), Arg(19)]hPTH(1-34)+ RP-cAMP group than in both the control group and [Gly(1), Arg(19)]hPTH(1-28)+RP-cAMP group (P<0.05), and its expression was the highest in PTH(1-34) group (P<0.05). RT-PCR of MC3T3-E1 cells yielded consist results with those in the primary osteoblasts, and the cells treated with Go6983 (a PKC inhibitor) did not show GR(1-28)- or GR(1-34)-mediated differential expression of CITED1. CONCLUSION: The activation of PLC-independent protein kinase C signaling pathway of PTH enhances the expression of CITED1 in mouse osteoblasts to mediate the effect of PTH on bone metabolism, and this pathway is not dependent on the activation of PLC or PKA signaling.


Assuntos
Proteínas Nucleares/fisiologia , Osteoblastos/fisiologia , Hormônio Paratireóideo/fisiologia , Proteína Quinase C/fisiologia , Transdução de Sinais , Transativadores/fisiologia , Animais , Proteínas Reguladoras de Apoptose , Células Cultivadas , Indóis , Maleimidas , Camundongos , Camundongos Endogâmicos C57BL , Crânio , Fosfolipases Tipo C
9.
Nan Fang Yi Ke Da Xue Xue Bao ; 35(4): 594-7, 2015 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-25907952

RESUMO

OBJECTIVE: To establish rabbit model of scoliosis induced with stable asymmetric lumbar loads. METHODS: Scoliosis was induced in 10 two-month-old New Zealand rabbits using 316L stainless steel springs placed between the unilateral transverse processes of L2 and L5. Serial radiographs were documented before and at 1, 4, 8, 9 and 12 weeks after the operation. At weeks, the rabbits were randomly divided into SR group (n=5) with the spring removed and SK group (n=5) without spring removal. RESULTS: All the rabbits survived the experiment with Cobb angle all greater than 10 degree at the end of the experiment. Significant changes were found in the Cobb angles and kyphotic angles at 1, 4 and 8 weeks after the operation (P<0.05). At 8 weeks, the Cobb angle, the kyphotic angle and the length of the spring were similar between SR and SK groups (P>0.05), and in the 4 weeks following spring removal in SR group, the Cobb angle and the kyphosis decreased significantly compared with those in SK group (P<0.05). Micro-CT showed that the BV/TV of the concave side was greater than that of the convex side. The length of the spring did not show obvious changes during the experiment (P>0.05). CONCLUSIONS: Asymmetric lumbar loading is a convenient, time-saving, and highly reproducible approach for establishing rabbit models of scoliosis.


Assuntos
Modelos Animais de Doenças , Escoliose/fisiopatologia , Coluna Vertebral/patologia , Animais , Coelhos
10.
Nan Fang Yi Ke Da Xue Xue Bao ; 31(11): 1867-70, 2011 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-22126767

RESUMO

OBJECTIVE: To establish a sensitive and direct method for detecting the activation of protein kinase C (PKC) using fluorescence resonance energy transfer (FRET) technique. METHODS: HEK293 cells were transfected with C kinase activity reporter (CKAR) plasmid or/and parathyroid receptor 1 plasmid , and after incubation for 72 h, the fluorescence resonance energy transfer was measured with or without parathyroid or TPA stimulation. RESULTS: TPA reduced the efficiency of FRET and increased the emission ratio of CFP/YFP (C/Y) in HEK293 cells transfected with CKAR. PTH(1-34) could increase the emission ratio of C/Y in HEK293 cells co-transfected with CKAR and PTHR1 but not in cells transfected with CKAR. CONCLUSION: FRET analysis using CKAR can be utilized to detect the activation of PKC, which provides a useful means for studying the signaling pathways associated with PKC.


Assuntos
Transferência Ressonante de Energia de Fluorescência/métodos , Proteína Quinase C/metabolismo , Proteínas de Bactérias/química , Ativação Enzimática , Genes Reporter , Células HEK293 , Humanos , Proteínas Luminescentes/química
11.
Nan Fang Yi Ke Da Xue Xue Bao ; 29(4): 729-31, 2009 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-19403407

RESUMO

OBJECTIVE: To determine the concentrations of interleukin-18 (IL-18), IL-6, IL-8, and prostaglandin E2 (PGE2) in the synovial fluid in patients with osteoarthritis (OA), and explore the role of IL-18 in the pathogenesis of OA. METHODS: The synovial fluid was collected from 30 patients with knee OA, and the concentrations of IL-18 and the other cytokines were measured using enzyme-linked immunosorbent assay (ELISA). A linear regression was performed between IL-18 and the other cytokines. RESULTS: The average IL-18 and PGE2 concentrations were 220-/+304 pg/ml and 89-/+104 pg/ml in the synovial fluid, respectively, and the two cytokines showed a positive correlation in the synovial fluid (r=0.628, P=0.001). The IL-18 concentration was also correlated to the concentrations of IL-6 (1200-/+1587 pg/ml, n=22; r=0.590, P=0.008) and IL-8 (5190-/+6024 pg/ml, n=9; r=0.776, P=0.014). CONCLUSION: IL-18 can promote PGE2 production, which causes cartilage degradation in OA, thus therapies targeting this cytokine may prove an effective approach to early OA treatment.


Assuntos
Interleucina-18/metabolismo , Osteoartrite/metabolismo , Líquido Sinovial/metabolismo , Idoso , Dinoprostona/biossíntese , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
12.
Zhonghua Yi Xue Za Zhi ; 83(10): 853-8, 2003 May 25.
Artigo em Chinês | MEDLINE | ID: mdl-12895338

RESUMO

OBJECTIVE: To establish recombinant adenovirus highly expressing genes of insulin-like growth factor-1 (IGF-1), platelet-derived growth factor-AA (PDGF-AA), and tumor growth factor beta1 (TGF beta 1) to be used in gene therapy. METHODS: Normal human osteoblasts were obtained from cancellous bone of lilium left from bone grafting and then cultured. Total RAN was extracted and IGF-1, PDGF-AA, and TGF beta 1 cDNAs was obtained by RT-PCR. Vector p-Shuttle containing these cNDAs was cloned into adenovirus and then the recombinant adenovirus was transfected into HEK293 cells. Recombinant adenovirus containing reporter gene LacZ was used in control group. Western blotting was used to detect the expression of these growth factors. Human osteoblasts were cultured and transfected with recombinant adenovirus. MTT method was used to detect the proliferation of the cells. Paranitrophenol method was used to examine the activity of alkaline phosphatase (ALP) in osteoblasts. RESULTS: Expression of IGF-1, PDGF-AA, and TGF beta 1 cDNA and expression of IGF-1, PDGF-AA, and TGF beta 1 proteins were found in HEK293 cells transfected with the recombinant adenovirus and not in the control HEK293 cells. The proliferation and ALP activity of osteoblasts transfected with the recombinant virus were significantly increased in comparison with those of the control osteoblasts (all P < 0.010). Immunohistochemical staining showed significant brown particles in the osteoblasts transfected with the recombinant virus and none in the control osteoblasts. CONCLUSION: The recombinant adenovirus thus constructed expresses the proteins of several growth factors with bioactivity in human cells and can be used as a satisfactory gene tool.


Assuntos
Adenoviridae/genética , Vetores Genéticos/genética , Fator de Crescimento Insulin-Like I/genética , Fator de Crescimento Derivado de Plaquetas/genética , Fator de Crescimento Transformador beta/genética , Sequência de Bases , Clonagem Molecular , Terapia Genética , Humanos , Dados de Sequência Molecular , Osteoblastos/metabolismo , Transfecção , Fator de Crescimento Transformador beta1
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...