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1.
J Hazard Mater ; 382: 121027, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31446346

RESUMO

Developing inexpensive and stable photocatalysts without noble metals, yet remarkably enhancing the photocatalytic activities, is highly needed. Here, a novel carbon and cerium co-doped porous g-C3N4 (C/Ce-CN) has been successfully prepared through thermal polymerization of the supramolecular aggregation. The morphologies, chemical structures, optical and photoelectrochemical properties of the synthesized photocatalysts were analyzed via a series of characterization measurements. Experimental results indicated that C/Ce-CN showed remarkably enhanced photocatalytic activity of TC and RhB degradation, which is about 2.6 and 2.4 times higher than that of pristine CN, and it also exhibited a good stability. Compared with bare CN, the enhanced performance of C/Ce-CN is mainly attributed to the stronger utilization rate of visible light, the rapider charge transfer rate, the longer lifetime of carriers and the larger surface specific area. The main intermediates in degradation process of antibiotics were tested by the HPLC-MS. Finally, the possible photocatalytic degradation pathways and mechanisms were proposed.

2.
J Hazard Mater ; 382: 121033, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31561196

RESUMO

The pyrolytic production of Fe-enriched composite biochar is receiving increasing attention. However, understanding of the environmental risk from the polycyclic aromatic hydrocarbons (PAHs) potentially generated during composite biochar production is lacking. This study investigated the formation of PAHs from the pyrolysis of barley straw impregnated with FeCl3 or Fe(NO3)3 at 350 °C, 500 °C, and 650 °C. The total amount of PAHs formation increased with increasing heating temperature. Most of the PAHs were concentrated in bio-oil (72.7-94.6%), with only a small fraction retained in biochar (1.7-11.1%) and in biogas (2.2-16.2%). Preloading FeCl3 or Fe(NO3)3 onto the biomass greatly reduced PAH formation by up to 33% and 21%, respectively, compared to that obtained with biomass alone. The suppressed formation of PAHs was due to the generation of more reductive forms of Fe, such as Fe0 and FeO, in the O2-starved pyrolysis atmosphere, which reduced C2H2 and C6H5OH, two important PAH precursors in hydrogen abstraction acetylene addition reactions. Although Fe loading reduced the amounts of PAHs in biochar, the toxic equivalent value increased because Fe induced more accumulation of high-molecular-weight PAHs in the biochar. This study proved that Fe loading suppresses PAH generation during biomass pyrolysis, which can guide the design of composite biochar production.

3.
Biomaterials ; 228: 119579, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31698227

RESUMO

Juvenile chondrocytes are robust in regenerating articular cartilage, but their clinical application is hindered by donor scarcity. Stem cells offer an abundant autologous cell source but are limited by slow cartilage deposition with poor mechanical properties. Using 3D co-culture models, mixing stem cells and chondrocytes can induce synergistic cartilage regeneration. However, the resulting cartilage tissue still suffers from poor mechanical properties after prolonged culture. Here we report a microribbon/hydrogel composite scaffold that supports synergistic interactions using co-culture of adipose-derived stem cells (ADSCs) and neonatal chondrocytes (NChons). The composite scaffold is comprised of a macroporous, gelatin microribbon (µRB) scaffolds filled with degradable nanoporous chondroitin sulfate (CS) hydrogel. We identified an optimal CS concentration (6%) that best supported co-culture synergy in vitro. Furthermore, 7 days of TGF-ß3 exposure was sufficient to induce catalyzed cartilage formation. When implanted in vivo, µRB/CS composite scaffold supported over a 40-fold increase in compressive moduli of cartilage produced by mixed ADSCs/NChons to ~330 kPa, which surpassed even the quality of cartilage produced by 100% NChons. Together, these results validate µRB/CS composite as a promising scaffold for cartilage regeneration using mixed populations of stem cells and chondrocytes.

4.
Inorg Chem ; 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31680518

RESUMO

A novel macrocyclic 1,7-dioxa-[2.1.1]-(2,6)-pyridinophane ligand has been synthesized and crystallographically characterized. Two derived metal complexes, dichloropalladium(II) and chlorocopper(I), were prepared. In the palladium(II) complex LPdCl2, both in the solid state, according to its crystallographic characterization, and in CH2Cl2 solutions at -40 °C, according to 1H NMR spectroscopy, the ligand adapts a C1-symmetric κ2-N,N-coordination mode in which the metal atom binds to two nonequivalent pyridine fragments of the macrocycle. The complex is fluxional at 20 °C. In the crystalline copper(I) complex LCuCl, the macrocyclic ligand is also κ2-N,N-coordinated to the metal, but it utilizes two equivalent pyridine fragments for the binding. The copper(I) complex is fluxional in CH2Cl2 solutions in the temperature range between 20 and -70 °C and is proposed to be involved in a fast intermolecular macrocyclic ligand exchange which is slowed down below -40 °C. DFT calculations predict a lower thermodynamic stability of the dioxapyridinophane-derived complexes LPdCl2 and LCuCl, as compared to their [2.1.1]-(2,6)-pyridinophane analogs containing bridging CH2 groups instead of the oxygen atoms. The electron poor dioxapyridinophane chlorocopper(I) complex, in combination with NaBArF4 (BArF4 = tetrakis[3,5-bis(trifluoromethyl)phenyl]borate) in dichloromethane solutions, can serve as an efficient catalyst for aziridination of various olefins with PhINTs at 0-22 °C.

5.
J Vet Pharmacol Ther ; 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31691990

RESUMO

The previously adopted marker residue for florfenicol (FF) in China was only florfenicol amine (FFA); however, the marker residue has been changed to FF plus FFA since the end of 2017. The previous official withdrawal period determined based on the only concentration of FFA may no longer be suitable. Therefore, the present study aimed to determine the depletion profiles of FF and FFA and further calculate the withdrawal period in the crucian carp (Carassius auratus) based on the new marker residues. Florfenicol was intramuscularly administered at 10 mg/kg bodyweight daily for five consecutive days to crucian carps reared in freshwater at 10°C. After the last dose, plasma and tissue samples were randomly collected from 10 fish at different time points. The FF and FFA concentrations were simultaneously determined by high-performance liquid chromatography (HPLC) with a fluorescence detector and further subjected to noncompartmental analysis. The elimination half-life (h) of FF in different tissues decreased as follows: liver (39.1) > kidney (36.3) > skin plus muscle (34.6) > plasma (31.7), whereas that of FFA decreased as follows: kidney (41.4) > skin plus muscle (39.4) > liver (39.3) > plasma (35.7). Considering a maximum residue limit of 1 µg/g for the total concentration of FF and FFA in the skin plus muscle, a withdrawal period of 6 days was calculated based on the upper limit of the one-sided 95% confidence interval.

6.
Sensors (Basel) ; 19(21)2019 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-31684132

RESUMO

Bioradar-based noncontact breathing detection technology has been widely studied due to its superior detection performance. In this paper, a breath detection mechanism based on the change in radar cross section (RCS) is proposed by using a forward scatter radar and the deduction of the mathematical model of the received signal. Furthermore, we completed human breathing detection experiments in an anechoic chamber and in an ordinary chamber; we obtained the breathing rate through envelope detection in cases where the human orientation angle was 0, 30, 60, and 90°. The analysis of the measured data shows that the theoretical model fits well with the measured results. Compared with the existing single-base radar detection schemes, the proposed scheme can detect human respiratory rates in different orientations.

7.
J Hazard Mater ; : 121455, 2019 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-31668763

RESUMO

Biochar as a soil amendment could be involved in redox process of elements which would be affected by soil-redox-active components including minerals and organic acids. This study evaluated the effects of Fe mineral and lactate on reducing capacity of biochar for Cr(VI) reduction and the underlying mechanisms. Fe minerals inhibited the reduction of Cr(VI) by biochar, with the decrease of Cr(VI) reduction rate constant obtained by pseudo first-order reaction model from 2.18 to 2.47 × 10-2 h-1 to 0.71-1.51 × 10-2 h-1. The decrease of reduction rate constant was because (1) the loss of electron donating moieties in biochar; and (2) inhibition of electron transfer between biochar and Cr(VI) due to surface coverage by biochar-Fe complex. However, the coexistence of Fe minerals with lactate enhanced the reduction of Cr(VI) by biochar, with the rate constant increasing from 2.58 to 3.05 × 10-2 h-1 to 2.91-27.2 × 10-2 h-1. The positive effect was also attributed to two reasons: (1) lactate can decrease the surface Fe-coverage of biochar through chelating process; (2) electron from lactate can be shuttled by Fe(II) and thus enhancing the Cr(VI) reduction. Our results revealed that different soil redox-active components could have varying effects on biochar amendment for Cr(VI) reduction, which should be further considered during the application of biochar.

8.
Mol Oncol ; 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31670863

RESUMO

Hepatocellular carcinoma (HCC), with its ineffective therapeutic options and poor prognosis, represents a global threat. In the present study, we show that RAD52 motif 1 (RDM1), a key regulator of DNA double-strand break repair and recombination, is downregulated in HCC tissues and suppresses tumor growth. In clinical HCC samples, low expression of RDM1 correlates with larger tumor size, poor tumor differentiation and unfavorable survival. In vitro and in vivo data demonstrate that knockdown of RDM1 increases HCC cell proliferation, colony formation and cell population at G2/M phase, whereas RDM1 overexpression results in the opposite phenotypes. Mechanistically, RDM1 binds to the tumor suppressor p53 and enhances its protein stability. In the presence of p53, RDM1 suppresses the phosphorylation of Raf and ERK. Overexpression of p53 or treatment with ERK inhibitor significantly abolishes cell proliferation induced by the depletion of RDM1. In addition, overexpression of methyltransferase-like 3 (METTL3) markedly induces N6-methyladenosine (m6 A) modification of RDM1 mRNA and represses its expression. Taken together, our study indicates that RDM1 functions as a tumor suppressor and may be a potential prognostic and therapeutic factor for HCC.

9.
Int J Radiat Biol ; : 1-23, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31729901

RESUMO

Purpose: It is reported inflammatory cytokine interleukin-8 (IL-8) could predict radiation-induced lung toxicity (RILT). RILT is believed to be a consequence of a cascade of cytokine production. It is considered that vascular endothelial cell and macrophages are the mainly source of cytokines. This study was investigated the production of IL-8 from cancer cells induced by X-rays may involve in the radiation-induced inflammation.Materials and methods: We analyzed IL-8 in human lung cancer cell lines after expose to X-rays, and we also detect IL-8 in HUVEC cells and THP1 cells as endothelial cell and macrophage model to identify the change in normal cells after expose. Furthermore, we added the inhibitors to the culture with or without radiation to identify the role of MAPK and NF-κB pathways on the radiation-induced secretion of IL-8.Results: Radiation could induce IL-8 production both in non-lung cancer cells (HUVECs and THP1 cells) and in lung cancer cells (A549 cells, H446 cells, PC-9 cells). Simultaneously, radiation activated p38/MAPK and NF-𝜅B signal pathways in lung cancer cells. Moreover, p38/MAPK inhibitor SB203580 and NF-κB inhibitor BAY11-7082 could block the IL-8 up-regulated by X-rays but JNK inhibitor SP600125, ERK inhibitor U0126, ROS Scavenger NAC could not inhibit this phenomenon.Conclusions: X-rays could induce IL-8 production in lung cancer cells, which may be related to the activation of p38/MAPK and NF-κB signaling pathway, providing a new point for elucidating the mechanism of radiation pneumonitis.

10.
BMC Pediatr ; 19(1): 403, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31684894

RESUMO

BACKGROUND: The aim of this study was to follow the growth and hematological indicators of preterm infants during their first year. METHODS: Neonates below 37 gestational weeks had routine follow-ups up through 1 year from January 2012 to December 2015 at West China 2nd University Hospital, Sichuan University. Weight, length and head circumference (HC) were measured monthly during the first 6 months, followed by monitoring every second month until 12 months. The catch-up growth defined as a gain of Z-score > 0.67 according to previous study. All preterm infants were prescribed iron prophylaxis based on national guideline. The hemoglobin concentration was examined at 6 and 12 months. RESULTS: Altogether, 132 very-low-birth-weight (VLBW), 504 low-birth-weight (LBW) and 198 normal-birth-weight (NBW) infants were followed. The rates of catch-up growth for weight, length and HC 12 months of corrected age (CA) were 22.6, 29.1 and 14.6%, respectively. SGA and VLBW infants showed higher catch-up growth rates. The overall prevalence of anemia was 6.8% at 6 months and 7.8% at 12 months. The Z-scores for weight-for-length, length and HC were lower in the VLBW and SGA preterm infant groups than in the other preterm groups throughout the first year of life. The incidences of stunting, microcephaly and wasting changed from 5, 1.3 and 3.7% to 2, 1.1, 0.9 and 2.4%, respectively, during the first year. However, the incidences of wasting and stunting were higher for the VLBW infants than for the LBW and NBW infants at 12 months (9.3% vs. 1.4%, p < 0.01; 9.3% vs. 1%, p < 0.01,respectively; 4.7% vs. 0.8%, p < 0.01, 4.7% vs. 0%, p < 0.01,respectively). Similar results were observed between SGA and AGA infants (8.7% vs. 1.5%, p < 0.01; 5.8% vs. 0.4%, p < 0.01). Logistic regression revealed SGA and VLBW as risk factors for poor growth (WLZ < -2SD) at 12 months (OR = 5.5, 95% CI: 2.1-14.8, p < 0.01: OR = 4.8, 95% CI: 1.8-12.8, p < 0.01, respectively). CONCLUSION: The VLBW and SGA preterm infants showed significant catch-up growth during their first year of life. However, SGA and VLBW were risk factors for poor growth during the preterm infants' first year of life. Prophylactic iron supplementation in preterm infants appears to reduce the prevalence of anemia.

11.
Sci Adv ; 5(10): eaaw4292, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31692762

RESUMO

Enhancing the hydrodynamic interfacial mobility of bubbles and droplets in multiphase systems is expected to reduce the characteristic coalescence times and thereby affect the stability of gas or liquid emulsions that are of wide industrial and biological importance. However, by comparing the controlled collision of bubbles or water droplets with mobile or immobile liquid interfaces, in a pure fluorocarbon liquid, we demonstrate that collisions involving mobile surfaces result in a significantly stronger series of rebounds before the rapid coalescence event. The stronger rebound is explained by the lower viscous dissipation during collisions involving mobile surfaces. We present direct numerical simulations to confirm that the observed rebound is enhanced with increased surface mobility. These observations require a reassessment of the role of surface mobility for controlling the dynamic stability of gas or liquid emulsion systems relevant to a wide range of processes, from microfluidics and pharmaceuticals to food and crude oil processing.

12.
Blood ; 2019 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-31697823

RESUMO

To study the mechanisms of relapse in acute lymphoblastic leukemia (ALL), we performed whole-genome sequencing of 103 diagnosis-relapse-germline trios and ultra-deep sequencing of 208 serial samples in 16 patients. Relapse-specific somatic alterations were enriched in 12 genes (NR3C1, NR3C2, TP53, NT5C2, FPGS, CREBBP, MSH2, MSH6, PMS2, WHSC1, PRPS1, and PRPS2) involved in drug response. Their prevalence was 17% in very early relapse (<9 months from diagnosis), 65% in early relapse (9-36 months), and 32% in late relapse (>36 months) groups. Convergent evolution, where multiple subclones harbor mutations in the same drug resistance gene, was observed in six relapses and confirmed by single-cell sequencing in one case. Mathematical modeling and mutational signature analysis indicated that early relapse resistance acquisition was frequently two-step process where a persistent clone survived initial therapy, and later acquired bona fide resistance mutations during therapy. In contrast, very early relapses arose from pre-existing resistant clone(s). Two novel relapse-specific mutational signatures, one of which was caused by thiopurine treatment based on in vitro drug exposure experiments, were identified in early and late relapses but were absent from 2,540 pan-cancer diagnosis samples and 129 non-ALL relapses. The novel signatures were detected in 27% of relapsed ALLs and were responsible for 46% of acquired resistance mutations in NT5C2, PRPS1, NR3C1, and TP53. These results suggest that chemotherapy-induced drug resistance mutations facilitate a subset of pediatric ALL relapses.

13.
Insect Biochem Mol Biol ; : 103261, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31698082

RESUMO

A network of serine proteases (SPs) and their non-catalytic homologs (SPHs) activates prophenoloxidase (proPO), Toll pathway, and other insect immune responses. However, integration and conservation of the network and its control mechanisms have not yet been fully understood. Here we present evidence that these responses are initiated through a conserved serine protease and negatively regulated by serpins in two species, Manduca sexta and Anopheles gambiae. We have shown that M. sexta serpin-12 reduces the proteolytic activation of HP6, HP8, proPO activating proteases (PAPs), SPHs, and POs in larval hemolymph, and we hypothesized that these effects are due to the inhibition of the immune pathway-initiating protease HP14. To test whether these changes are due to HP14 inhibition, we isolated a covalent complex of HP14 with serpin-12 from plasma using polyclonal antibodies against the HP14 protease domain or against serpin-12, and confirmed formation of the complex by 2D-electrophoresis, immunoblotting, and mass spectrometry. Upon recognition of bacterial peptidoglycans or fungal ß-1,3-glucan, the zymogen proHP14 became active HP14, which formed an SDS-stable complex with serpin-12 in vitro. Activation of proHP21 by HP14 was suppressed by serpin-12, consistent with the decrease in steps downstream of HP21, proteolytic activation of proPAP3, proSPH1/2 and proPO in hemolymph. Guided by the results of phylogenetic analysis, we cloned and expressed A. gambiae proSP217 (an ortholog of HP14) and core domains of A. gambiae serpin-11 and -17. The recombinant SP217 zymogen became active during expression, with cleavage between Tyr394 and Ile395. Both MsHP14 and AgSP217 cleaved MsSerpin-12 and AgSRPN11 at Leu*Ser (P1*P1') and formed complexes in vitro. ProPO activation in M. sexta plasma increased after recombinant AgSP217 had been added, indicating that it may function in a similar manner as the endogenous initiating protease HP14. Based on these data, we propose that inhibition of an initiating modular protease by a serpin may be a common mechanism in holometabolous insects to regulate proPO activation and other protease-induced immune responses.

14.
Anal Chem ; 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31698900

RESUMO

Phosphate-containing metabolites and alkaline phosphatase (ALP) activity are useful biomarkers for many types of diseases. However, there are few straightforward, sensitive, and efficient colorimetric methods for the quantification of them only when resorting to unstable transition metal ions or specially designed organic substrates. Herein, we have demonstrated that histidine-protected gold nanoclusters (His-AuNCs) possess intrinsic peroxidase-like activity with Au atom facilitated formation of superoxide anions (O2•-) and their electron transfer ability. More interestingly, phosphate-containing metabolites can severely inhibit the peroxidase-like activity of His-AuNCs by blocking the generation of O2•- and electron transfer, and then ALP is able to restore the inhibition process through hydrolyzing the phosphate-containing metabolites. Therefore, using peroxidase-triggered chromogenic reaction of 3,3',5,5'-tetramethylbenzidine (TMB) as an amplifier, a colorimetric on-off-on switch has been developed for sensing phosphate-containing metabolites and ALP based on the logical regulation of such deactivation and reactivation processes for the first time. According to the intrinsic mimic enzyme-catalyzed amplification and clear response mechanism, our colorimetric assay exhibits excellent sensitivity, selectivity, and sensing performance. Furthermore, on the basis of the proposed colorimetric sensors, a combinatorial "NOR+IMPLICATION" logic gate is further rationally constructed.

15.
Langmuir ; 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31721588

RESUMO

As an important subclass of MOFs, ZIF-8, built from 2-methylimidazole and Zn(NO3)2•6H2O, possesses the excellent biocompatibility and high stability in aqueous solution. Recently, it has been found that ZIF-8 can efficiently adsorb DNA, and quench the adsorbed fluorophores to a large extent. These properties make it possible to prepare DNA-based optical sensors using ZIF-8. Although practical analytical applications are being demonstrated, the basic understanding of the binding between ZIF-8 and DNA in solution has received relatively little attention. In this work, we report that the adsorption of 12-, 18-, 24-, and 36-mer single-stranded DNAs on ZIF-8 are affected by several factors. It is found from the outcomes that shorter DNAs are adsorbed more rapidly to the surface of ZIF-8. On the other hand, desorption of the probe DNA can be achieved using complementary strand DNA to restore the fluorescence value. Furthermore, the salt contributes to adsorption to some extent. These findings are important for further understanding of the interactions between DNA and ZIF-8 and for the optimization of DNA and MOF-based devices and sensors.

16.
J Synchrotron Radiat ; 26(Pt 6): 2075-2080, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31721753

RESUMO

The upgrade of the laser pump time-resolved X-ray probes, namely time-resolved X-ray absorption spectroscopy (TR-XAS) and X-ray diffraction (TR-XRD), implemented at the Beijing Synchrotron Radiation Facility, is described. The improvements include a superbunch fill, a high-efficiency fluorescence collection, an efficient spatial overlap protocol and a new data-acquisition scheme. After upgrade, the adequate TR-XAS signal is now obtained in a 0.3 mM solution, compared with a 6 mM solution in our previous report. Furthermore, to extend application in photophysics, the TR-XAS probe is applied on SrCoO2.5 thin film. And for the first time, TR-XAS is combined with TR-XRD to simultaneously detect the kinetic trace of structural changes in thin film.

17.
J Orthop Surg Res ; 14(1): 364, 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31727107

RESUMO

BACKGROUND: To compare the efficacy of the operative techniques, complications, reduction quality and hip functional recovery by using the supra-ilioinguinal approach and the modified Stoppa approach for the management of acetabular fractures. METHODS: A consecutive cohort of 60 patients from September 2014 to October 2017 with displaced acetabular fractures involving the quadrilateral plate were treated operatively with supra-ilioinguinal approach (group A) and modified Stoppa approach (group B), respectively. There were 36 patients in group A and 24 patients in group B. The surgical details, complications, radiographic and clinical results were recorded. The quality of reduction was assessed by measuring the residual step and gap displacement of postoperative CT with a standardized digital method. RESULTS: The complications, reduction quality (gaps and steps) and hip function recovery had no significant statistical difference in approaches. The mean operative time was shorter and the mean intraoperative haemorrhage was less in group A. There were statistical differences in the operative time (P = 0.025) and intraoperative haemorrhage (P = 0.003) between the supra-ilioinguinal approach and the modified Stoppa approach. CONCLUSION: Compared to the modified Stoppa approach, the supra-ilioinguinal approach provides a closer visualization to the quadrilateral plate, the operative time was shorter and the intraoperative haemorrhage was clearly less. It is at least equal to or could be a better choice to deal with complicated acetabular fractures especially involving the quadrilateral plate and the anterior one third of the iliac bone.

18.
Cell Commun Signal ; 17(1): 147, 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31727175

RESUMO

Neutrophils have long been considered as cells playing a crucial role in the immune defence against invading pathogens. Accumulating evidence strongly supported the direct and indirect regulatory effects of neutrophils on adaptive immunity. Exogenous cytokines or cytokines produced in an autocrine manner as well as a cell-to-cell contact between neutrophils and T cells could induce the expression of MHC-II and costimulatory molecules on neutrophils, supporting that neutrophils may function as antigen-presenting cells (APCs) in respects of presenting antigens and activating T cells. In addition to the inflammatory roles, neutrophils also have the propensity and ability to suppress the immune response through different mechanisms. In this review, we will mainly highlight the heterogeneity and functional plasticity of neutrophils and the antigen-presenting capacity of different neutrophil subsets. We also discuss mechanisms relevant to the regulatory effects of neutrophils on adaptive immunity. Understanding how neutrophils modulate adaptive immunity may provide novel strategies and new therapeutic approaches for diseases associated with neutrophils.

19.
J Biosci Bioeng ; 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31708427

RESUMO

Okara, despite being a soybean processing by-product, still holds many nutrients. Thus, considerable attention has been recently paid to its reuse. In this study, solid-state fermentation was performed using Ganoderma lucidum and Lentinus edodes. Antioxidant activity and bioactive compound levels in G. lucidum-fermented okara (GLFO) and L. edodes-fermented okara (LEFO) were assayed. Antiosteoporosis bioactivity was evaluated using an animal model. The results demonstrated that solid-state fermentation significantly improved the antioxidant activity and bioactive compound levels. Furthermore, GLFO and LEFO increased trabecular bone volume, although only the GLFO-treated group exhibited significantly improved trabecular separation compared with the bilateral ovariectomy-treated control group. GLFO-related outcomes were superior to those of LEFO. The results demonstrate that okara products are effective for treating postmenopausal osteoporosis in humans.

20.
Artigo em Inglês | MEDLINE | ID: mdl-31703302

RESUMO

Methadone maintenance treatment (MMT) is an important approach to address opioid dependence. However, MMT clinics usually report high attrition rates. Our previous randomized controlled trial demonstrated additional psycho-social services delivered by social workers could reduce attrition rates compared to MMT alone. This study aimed to evaluate the effectiveness of psycho-social service in a real-world context. A quasi-experimental design and propensity score matching was adopted. 359 clients were recruited from five MMT clinics in Guangzhou from July 2013 to April 2015. One 20-minute counseling session was offered to the control group after enrolment. The intervention group received six sessions of psycho-social services. The baseline characteristics were unbalanced between two arms in the original sample. After propensity score matching, 248 participants remained in the analysis. At month six, the intervention group had a lower attrition rate [intervention (39.5%) versus control (52.4%), P = 0.041], higher monthly income [monthly income of 1000 CNY or higher: intervention (55.9%) versus control (39.0%), P = 0.028)], higher detoxification intention score [full intention score: intervention (51.6%) versus control (32.5%), P = 0.012)], higher family support in MMT participation [intervention (77.9%) versus control (61.4%), P = 0.049)]. This study demonstrated that psycho-social services delivered by social workers can reduce MMT clients' attrition and improve their well-being in real-world settings.

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