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1.
Biomaterials ; 228: 119579, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31698227

RESUMO

Juvenile chondrocytes are robust in regenerating articular cartilage, but their clinical application is hindered by donor scarcity. Stem cells offer an abundant autologous cell source but are limited by slow cartilage deposition with poor mechanical properties. Using 3D co-culture models, mixing stem cells and chondrocytes can induce synergistic cartilage regeneration. However, the resulting cartilage tissue still suffers from poor mechanical properties after prolonged culture. Here we report a microribbon/hydrogel composite scaffold that supports synergistic interactions using co-culture of adipose-derived stem cells (ADSCs) and neonatal chondrocytes (NChons). The composite scaffold is comprised of a macroporous, gelatin microribbon (µRB) scaffolds filled with degradable nanoporous chondroitin sulfate (CS) hydrogel. We identified an optimal CS concentration (6%) that best supported co-culture synergy in vitro. Furthermore, 7 days of TGF-ß3 exposure was sufficient to induce catalyzed cartilage formation. When implanted in vivo, µRB/CS composite scaffold supported over a 40-fold increase in compressive moduli of cartilage produced by mixed ADSCs/NChons to ~330 kPa, which surpassed even the quality of cartilage produced by 100% NChons. Together, these results validate µRB/CS composite as a promising scaffold for cartilage regeneration using mixed populations of stem cells and chondrocytes.

2.
Surg Oncol Clin N Am ; 29(1): 127-144, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31757309

RESUMO

Oral squamous cell carcinoma (OSCC) is one of the leading cancers in the world. OSCC patients are managed with surgery and/or chemoradiation. Prognoses and survival rates are dismal, however, and have not improved for more than 20 years. Recently, the concept of precision medicine was introduced, and the introduction of targeted therapeutics demonstrated promising outcomes. This article reviews the current understanding of initiation, progression, and metastasis of OSCC from both genetic and epigenetic perspectives. In addition, the applications and integration of omics technologies in biomarker discovery and drug development for treating OSCC are reviewed.

3.
Anal Chim Acta ; 1094: 142-150, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31761041

RESUMO

α-synuclein is a predominantly expressing neuronal protein for understanding the neurodegenerative disorders. A diagnosing system with aggregated α-synuclein encoded by SNCA gene is necessary to make the precautionary treatment against Parkinson's disease (PD). Herein, gold-nanourchin conjugated anti-α-synuclein antibody was desired as the probe and seeded on single-walled carbon nanotube (SWCN) integrated interdigitated electrode (IDE). The surface morphology of SWCN-modified IDE and gold urchin-antibody conjugates were observed under FESEM, FETEM and AFM, the existing elements were confirmed. Voltammetry analysis revealed that the limit of fibril-formed α-synuclein detection was improved by 1000 folds (1 fM) with gold-nanourchin-antibody modified surface, compared to the surface with only antibody (1 pM). Validating the interaction of α-synuclein by Enzyme-linked Immunosorbent Assay was displayed the detection limit as 10 pM. IDE has a good reproducibility and a higher selectivity on α-synuclein as evidenced by the interactive analysis with the control proteins, PARK1 and DJ-1.

4.
J Hazard Mater ; 382: 121027, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31446346

RESUMO

Developing inexpensive and stable photocatalysts without noble metals, yet remarkably enhancing the photocatalytic activities, is highly needed. Here, a novel carbon and cerium co-doped porous g-C3N4 (C/Ce-CN) has been successfully prepared through thermal polymerization of the supramolecular aggregation. The morphologies, chemical structures, optical and photoelectrochemical properties of the synthesized photocatalysts were analyzed via a series of characterization measurements. Experimental results indicated that C/Ce-CN showed remarkably enhanced photocatalytic activity of TC and RhB degradation, which is about 2.6 and 2.4 times higher than that of pristine CN, and it also exhibited a good stability. Compared with bare CN, the enhanced performance of C/Ce-CN is mainly attributed to the stronger utilization rate of visible light, the rapider charge transfer rate, the longer lifetime of carriers and the larger surface specific area. The main intermediates in degradation process of antibiotics were tested by the HPLC-MS. Finally, the possible photocatalytic degradation pathways and mechanisms were proposed.

5.
J Hazard Mater ; 382: 121033, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31561196

RESUMO

The pyrolytic production of Fe-enriched composite biochar is receiving increasing attention. However, understanding of the environmental risk from the polycyclic aromatic hydrocarbons (PAHs) potentially generated during composite biochar production is lacking. This study investigated the formation of PAHs from the pyrolysis of barley straw impregnated with FeCl3 or Fe(NO3)3 at 350 °C, 500 °C, and 650 °C. The total amount of PAHs formation increased with increasing heating temperature. Most of the PAHs were concentrated in bio-oil (72.7-94.6%), with only a small fraction retained in biochar (1.7-11.1%) and in biogas (2.2-16.2%). Preloading FeCl3 or Fe(NO3)3 onto the biomass greatly reduced PAH formation by up to 33% and 21%, respectively, compared to that obtained with biomass alone. The suppressed formation of PAHs was due to the generation of more reductive forms of Fe, such as Fe0 and FeO, in the O2-starved pyrolysis atmosphere, which reduced C2H2 and C6H5OH, two important PAH precursors in hydrogen abstraction acetylene addition reactions. Although Fe loading reduced the amounts of PAHs in biochar, the toxic equivalent value increased because Fe induced more accumulation of high-molecular-weight PAHs in the biochar. This study proved that Fe loading suppresses PAH generation during biomass pyrolysis, which can guide the design of composite biochar production.

6.
J Hazard Mater ; 383: 121157, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31518807

RESUMO

High doses of molybdenum (Mo) and cadmium (Cd) cause adverse reactions on animals, but the joint toxic effects of Mo and Cd on duck renal tubular epithelial cells are not fully illustrated. To investigate the combined effects of Mo and Cd on oxidative stress and mitochondrial apoptosis in primary duck renal tubular epithelial cells, the cells were either treated with (NH4)6Mo7O24·4H2O (480, 960 µM Mo), 3CdSO4·8H2O (2.5, 5.0 µM Cd) or combination of Mo and Cd for 12 h, and then the joint cytotoxicity was evaluated. The results demonstrated that Mo or/and Cd exposure could induce release of intracellular lactate dehydrogenase, reactive oxygen species generation, acidification, increase levels of malondialdehyde and [Ca2+]i, decrease levels of glutathione, glutathione peroxidase, catalase, superoxide dismutase, total antioxidant capacity, Na+/K+-ATPase, Ca2+-ATPase, and mitochondrial membrane potential; upregulate mRNA levels of Caspase-3, Bak-1, Bax, and cytochrome C, inhibit Bcl-2 mRNA level, and induce cell apoptosis in a dose-dependent manner. Furthermore, the changes of these indicators in co-treated groups were more remarkable. The results indicated that exposure to Mo or/and Cd could induce oxidative stress and apoptosis via the mitochondrial pathway in duck renal tubular epithelial cells and the two metals may have a synergistic effect.

7.
J Hazard Mater ; 383: 121170, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31522068

RESUMO

Nano zero-valent iron (nZVI), as a high-efficiency adsorbent for heavy metals, often suffers being oxidized and assembling together due to small size and super reactivity, further decreasing its adsorption performance and limiting application ranges. Herein, we have designed a novel adsorbent with high-dispersion nZVI stabilized by as-prepared artificial humic acid (AHA-nZVI) derived from hydrothermal humification (HTH) technology. Introduction of artificial humic acid (A-HA) can effectively reduce the oxidation and agglomeration of nZVI, leading to superior kinetic removal efficiency of Pb2+ (> 99.2%) and huge Langmuir removal capacity of 649.0 mg/g. The combination of nZVI and A-HA (contained abundant functional groups, i.e. -OH and -COOH) via C-O-Fe bonding makes nZVI have good dispersion and oxidation resistance. Multiple interaction mechanisms including reduction reaction, complexation and co-precipitation between heavy metals and AHA-nZVI samples are realized. Overall, AHA-nZVI is a promising material for high-performance heavy metal contaminated water treatment.

8.
JMIR Mhealth Uhealth ; 7(12): e13671, 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31789599

RESUMO

BACKGROUND: Sickle cell disease (SCD) is an inherited red blood cell disorder affecting millions worldwide, and it results in many potential medical complications throughout the life course. The hallmark of SCD is pain. Many patients experience daily chronic pain as well as intermittent, unpredictable acute vaso-occlusive painful episodes called pain crises. These pain crises often require acute medical care through the day hospital or emergency department. Following presentation, a number of these patients are subsequently admitted with continued efforts of treatment focused on palliative pain control and hydration for management. Mitigating pain crises is challenging for both the patients and their providers, given the perceived unpredictability and subjective nature of pain. OBJECTIVE: The objective of this study was to show the feasibility of using objective, physiologic measurements obtained from a wearable device during an acute pain crisis to predict patient-reported pain scores (in an app and to nursing staff) using machine learning techniques. METHODS: For this feasibility study, we enrolled 27 adult patients presenting to the day hospital with acute pain. At the beginning of pain treatment, each participant was given a wearable device (Microsoft Band 2) that collected physiologic measurements. Pain scores from our mobile app, Technology Resources to Understand Pain Assessment in Patients with Pain, and those obtained by nursing staff were both used with wearable signals to complete time stamp matching and feature extraction and selection. Following this, we constructed regression and classification machine learning algorithms to build between-subject pain prediction models. RESULTS: Patients were monitored for an average of 3.79 (SD 2.23) hours, with an average of 5826 (SD 2667) objective data values per patient. As expected, we found that pain scores and heart rate decreased for most patients during the course of their stay. Using the wearable sensor data and pain scores, we were able to create a regression model to predict subjective pain scores with a root mean square error of 1.430 and correlation between observations and predictions of 0.706. Furthermore, we verified the hypothesis that the regression model outperformed the classification model by comparing the performances of the support vector machines (SVM) and the SVM for regression. CONCLUSIONS: The Microsoft Band 2 allowed easy collection of objective, physiologic markers during an acute pain crisis in adults with SCD. Features can be extracted from these data signals and matched with pain scores. Machine learning models can then use these features to feasibly predict patient pain scores.

9.
Chemosphere ; 244: 125468, 2019 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-31790986

RESUMO

Niclosamide (NIC) is the most widely used molluscicides for preventing the occurrence of schistosomiasis disease, and its residues can be found in various environmental samples. However, the toxicity mechanism of NIC during early developmental stage remains largely unknown. In the present study, zebrafish embryos were acutely exposed to NIC at an environmentally realistic concentration (0 and 40 µg/L) until 120 h post-fertilization. Transcriptomic sequencing was performed to provide mechanistic insight into developmental impairment. Pathway enrichment analyses found that biological processes related to lipid metabolism were significantly affected in exposed zebrafish larvae. Consistently, biochemical measurements showed that NIC developmental exposure depleted lipid storage, elevated lipid utilization, but inhibited lipid synthesis. Furthermore, as characterized by pathway enrichment and hormonal levels, steroid hormone biosynthesis was also significantly disrupted by NIC exposure in zebrafish larvae, indicating the endocrine disrupting potential of NIC. Detoxifying phase I and II processes (e.g., metabolism, conjugation and elimination) were significantly activated by NIC exposure. Overall, our findings suggest that NIC developmental exposure at an environmentally realistic concentration disturbs the lipid metabolism, induces endocrine disruption and initiates detoxifying capacity in zebrafish larvae, which will provide preliminary clues for developmental toxicity mechanisms of NIC.

10.
Stem Cell Res Ther ; 10(1): 364, 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31791391

RESUMO

BACKGROUND: The limited proliferative ability of hepatocytes is a major limitation to meet their demand for cell-based therapy, bio-artificial liver device, and drug tests. One strategy is to amplify cells at the hepatoblast (HB) stage. However, expansion of HBs with their bipotency preserved is challenging. Most HB expansion methods hardly maintain the bipotency and also lack functional confirmation. METHODS: On the basis of analyzing and manipulating related signaling pathways during HB (derived from human induced pluripotent stem cells, iPSCs) differentiation and proliferation, we established a specific chemically defined cocktails to synergistically regulate the related signaling pathways that optimize the balance of HB proliferation ability and stemness maintenance, to expand the HBs and investigate their capacity for injured liver repopulation in immune-deficient mice. RESULTS: We found that the proliferative ability progressively declines during HB differentiation process. Small molecule activation of Wnt or inhibition of TGF-ß pathways promoted HB proliferation but diminished their bipotency, whereas activation of hedgehog (HH) signaling stimulated proliferation and sustained HB phenotypes. A cocktail synergistically regulating the BMP/WNT/TGF-ß/HH pathways created a fine balance for expansion and maintenance of the bipotency of HBs. After purification, colony formation, and expansion for 20 passages, HBs retained their RNA profile integrity, normal karyotype, and ability to differentiate into mature hepatocytes and cholangiocytes. Moreover, upon transplantation into liver injured mice, the expanded HBs could engraft and differentiate into mature human hepatocytes and repopulate liver tissue with restoring hepatocyte mass. CONCLUSION: Our data contribute to the understanding of some signaling pathways for human HB proliferation in vitro. Simultaneous BMP/HGF induction, activation of Wnt and HH, and inhibition of TGF-ß pathways created a reliable method for long-term stable large-scale expansion of HBs to obtain mature hepatocytes that may have substantial clinical applications.

11.
Water Environ Res ; 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31793104

RESUMO

As an emerging organic pollutant, microplastics in the ocean have been the subject of much study. However, there is a lack of research on freshwater environments, notably in sediment, China. Microplastics contamination in sediment of Lake Ulansuhai has been investigated in the study, and its concentration range was from 24 ± 7 n/kg to 14 ± 3 n/kg. Further, it exhibited a difference in spatial distribution with high content in the north zone of the lake far from the entrance of the drainage canal and it has a decreasing tendency with the flow from north to south in lake. Colored plastic particles acted as the dominated pollution type, and more than 79.69% of microplastics were smaller than 2 mm in size, existed as the form of fibers. FTIR results mirrored that the main types of microplastics were polyethylene, polyethylene terephthalate, polypropylene and polyvinyl chloride. Results obtained from SEM/EDS showed that there were cracks, holes and irregular curls on the surface of it, and these microplastics may provide attachment sites for other pollutants. The appearance of metallic elements such as Al, Ni and Fe showed that microplastics and other pollutants in Lake Ulansuhai have a combined effect, which may aggravate the degree of pollution in the lake.

12.
Histol Histopathol ; : 18188, 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31793657

RESUMO

Accumulating dysregulated lncRNAs have been demonstrated to execute vital functions in the pathogenesis and progress of gastric cancer (GC) through versatile molecular mechanisms. In this review, we classify the mechanisms of dysregulated lncRNAs in GC into several governing types according to their roles at molecular level. For each regulatory role, we illustrate several instructive examples and introduce significant effects of lncRNAs on cellular biological properties of GC. Besides, we summarize a group of lncRNA-signatures that are potential biomarkers in the prediction of prognosis for GC patients.

13.
Sci Rep ; 9(1): 18388, 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31804548

RESUMO

Sex ratio bias is common in migratory animals and can affect population structure and reproductive strategies, thereby altering population development. However, little is known about the underlying mechanisms that lead to sex ratio bias in migratory insect populations. In this study, we used Cnaphalocrocis medinalis, a typical migratory pest of rice, to explore this phenomenon. A total of 1,170 moths were collected from searchlight traps during immigration periods in 2015-2018. Females were much more abundant than males each year (total females: total males = 722:448). Sex-based differences in emergence time, take-off behaviour, flight capability and energy reserves were evaluated in a laboratory population. Females emerged 0.78 days earlier than males. In addition, the emigratory propensity and flight capability of female moths were greater than those of male moths, and female moths had more energy reserves than did male moths. These results indicate that female moths migrate earlier and can fly farther than male moths, resulting more female moths in the studied immigratory population.

14.
Sci Rep ; 9(1): 18460, 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31804588

RESUMO

In this paper, the Taklimakan Desert land surface emissivity measured by portable Fourier Transform thermal InfraRed spectroscopy (FTIR) was used to re-estimate Global LAnd Surface Satellite (GLASS) BroadBand Emissivity (BBE) and Moderate Resolution Imaging Spectroradiometer (MODIS) BBE optimal coefficients equations. In addition, the revised BBE equations of both GLASS and MODIS with the optimized coefficients were obtained. Comparing the FTIR and MODIS BBE data with the values retrieved before and after the revised GLASS BBE equation, it was found that the revised GLASS BBE equation is more accurate than the original one. First, according to the error analysis with FTIR data, the value of R2 is increased from 0.4 to 0.9, and the Root Mean Square Error (RMSE) and Bias are reduced by 1 and 3 magnitude orders, respectively. Second, with MODIS BBE data, the value of R2 is increased from 0.6 to 0.9, the RMSE and Bias are reduced by 1 and 2 magnitude orders, respectively. Finally, the Taklimakan Desert BBE was calculated using the revised GLASS BBE equation. The results showed that the BBE values are between 0.890 and 0.920 in the desert center, between 0.920 and 0.950 in the sparse vegetation areas, and between 0.950 and 0.980 in the oasis edges.

15.
Lung Cancer ; 139: 118-123, 2019 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-31775086

RESUMO

OBJECTIVES: The 2015 World Health Organization classification defines pulmonary large-cell neuroendocrine carcinoma (LCNEC) as a high-grade neuroendocrine carcinoma. However, the clinical characteristics and prognostic factors of pure LCNEC and combined LCNEC remain unclear. Hence, we performed a multi-center retrospective study to compare the clinical outcomes of pure versus combined LCNEC. MATERIALS AND METHODS: Data from 381 patients with pulmonary LCNEC admitted to 17 Chinese institutes between 2009 and 2016 were collected retrospectively. Clinical characteristics and prognosis were analyzed among patients receiving adjuvant (adjuvant group; n = 56) and first-line (first-line group; n = 146) chemotherapy, as well as among patients receiving small cell lung cancer (SCLC) and non-SCLC (NSCLC) chemotherapy regimens. The Kaplan-Meier method and multivariable Cox regression were used to identify clinicopathological variables that might influence patient outcomes. RESULTS: Expression levels of neuroendocrine markers (synaptophysin, chromogranin-A, CD56) were associated with patients' prognosis in the total study cohort. In the adjuvant group, median disease-free survival was non-significantly longer for SCLC-based regimens than for NSCLC-based regimens (P = 0.112). In the first-line group, median progression-free survival was significantly longer for SCLC-based regimens than for NSCLC-based regimens (11.5 vs. 7.2 months, P = 0.003). Among patients with combined LCNEC, adenocarcinoma was the most common combined component, accounting for 70.0 % of cases. Additionally, median overall survival was non-significantly shorter for combined LCNEC than for pure LCNEC (P = 0.083). CONCLUSION: The SCLC regimen is a more effective choice, as either first-line or adjuvant chemotherapy, when compared to the NSCLC regimen for LCNEC treatment. Further studies are needed to clarify the survival differences between patients with pure-, and combined LCNEC.

16.
Eur J Med Chem ; 185: 111803, 2019 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-31677447

RESUMO

A potent and novel MET inhibitor, 5-((4-((2-amino-3-chloropyridin-4-yl)oxy)-3-fluorophenyl)amino)-3-(4-fluorophenyl)-1,6-naphthyridin-4(1H)-ones (8), was designed and synthesized via a scaffold-hopping strategy of a 2,7-naphthyridinone MET kinase inhibitor 7. Lead compound 8 had good potency (IC50 of 9.8 nM), but unfavorable pharmacokinetic profiles (F = 12%, CL = 5.0 L/h/kg). Systematic structural optimization of compound 8 resulted in 9g (MET, IC50 = of 9.8 nM) with a comparable MET potency to that of compound 2 and a favorable pharmacokinetic profile (F = 63%, CL = 0.12 L/h/kg). Further study of the derivatization of N(1) amine group of 9g led to the discovery of 23a with good MET potency (IC50 of 7.1 nM), promising VEGFR-2 selectivity (3226-fold), and a markedly drug-likeness improvement (F = 57.7%, CL = 0.02 L/h/kg). The excellent VEGFR-2 selectivity and favorable drug-likeness of 23g suggest that the 1,6-naphthyridine moiety could be used as a new scaffold for kinase inhibitor discovery.

17.
Sensors (Basel) ; 19(21)2019 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-31684132

RESUMO

Bioradar-based noncontact breathing detection technology has been widely studied due to its superior detection performance. In this paper, a breath detection mechanism based on the change in radar cross section (RCS) is proposed by using a forward scatter radar and the deduction of the mathematical model of the received signal. Furthermore, we completed human breathing detection experiments in an anechoic chamber and in an ordinary chamber; we obtained the breathing rate through envelope detection in cases where the human orientation angle was 0, 30, 60, and 90°. The analysis of the measured data shows that the theoretical model fits well with the measured results. Compared with the existing single-base radar detection schemes, the proposed scheme can detect human respiratory rates in different orientations.

18.
J Hazard Mater ; : 121455, 2019 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-31668763

RESUMO

Biochar as a soil amendment could be involved in redox process of elements which would be affected by soil-redox-active components including minerals and organic acids. This study evaluated the effects of Fe mineral and lactate on reducing capacity of biochar for Cr(VI) reduction and the underlying mechanisms. Fe minerals inhibited the reduction of Cr(VI) by biochar, with the decrease of Cr(VI) reduction rate constant obtained by pseudo first-order reaction model from 2.18 to 2.47 × 10-2 h-1 to 0.71-1.51 × 10-2 h-1. The decrease of reduction rate constant was because (1) the loss of electron donating moieties in biochar; and (2) inhibition of electron transfer between biochar and Cr(VI) due to surface coverage by biochar-Fe complex. However, the coexistence of Fe minerals with lactate enhanced the reduction of Cr(VI) by biochar, with the rate constant increasing from 2.58 to 3.05 × 10-2 h-1 to 2.91-27.2 × 10-2 h-1. The positive effect was also attributed to two reasons: (1) lactate can decrease the surface Fe-coverage of biochar through chelating process; (2) electron from lactate can be shuttled by Fe(II) and thus enhancing the Cr(VI) reduction. Our results revealed that different soil redox-active components could have varying effects on biochar amendment for Cr(VI) reduction, which should be further considered during the application of biochar.

19.
Mol Oncol ; 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31670863

RESUMO

Hepatocellular carcinoma (HCC), with its ineffective therapeutic options and poor prognosis, represents a global threat. In the present study, we show that RAD52 motif 1 (RDM1), a key regulator of DNA double-strand break repair and recombination, is downregulated in HCC tissues and suppresses tumor growth. In clinical HCC samples, low expression of RDM1 correlates with larger tumor size, poor tumor differentiation and unfavorable survival. In vitro and in vivo data demonstrate that knockdown of RDM1 increases HCC cell proliferation, colony formation and cell population at G2/M phase, whereas RDM1 overexpression results in the opposite phenotypes. Mechanistically, RDM1 binds to the tumor suppressor p53 and enhances its protein stability. In the presence of p53, RDM1 suppresses the phosphorylation of Raf and ERK. Overexpression of p53 or treatment with ERK inhibitor significantly abolishes cell proliferation induced by the depletion of RDM1. In addition, overexpression of methyltransferase-like 3 (METTL3) markedly induces N6-methyladenosine (m6 A) modification of RDM1 mRNA and represses its expression. Taken together, our study indicates that RDM1 functions as a tumor suppressor and may be a potential prognostic and therapeutic factor for HCC.

20.
Chemosphere ; 243: 125364, 2019 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-31760285

RESUMO

Surplus nutrient load and complex migration and transformation processes are the challenges for water quality management in the peri-urban coastal watershed, leading to increasing concerns worldwide. We investigated the spatio-temporal variation of hydrogeochemical parameters in surface water of Jimei Lake watershed, and distinguished the sources and transformation of nitrate-N (NO3--N) using dual isotopes of nitrate (δ15N and δ18O in NO3-) with hydrogeochemical indicators. Principal component analysis (PCA) on hydrogeochemical parameters demonstrated that surface water was seriously polluted by nutrients, especially in the southeast of the downstream. There were signs of seawater intrusion and increased wastewater discharge in the mid-lower reaches with high ammonium concentrations. Nitrification occurred throughout the monitoring period with lower δ15N and δ18O values and NO3- derived from mixed pollution sources. Results of Bayesian model showed that dominant NO3- input originated from manure and sewage (M&S, 71% and 76% in the wet and dry season, respectively) and atmospheric deposition (22% and 16%, respectively). This result implied that the controls and treatment of M&S discharges are essential to alleviate of NO3- pollution. The proposed method is helpful to understand the origins of NO3- and may be suitable to develop measures for the reducing of nitrogen loadings in the peri-urban watershed.

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