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1.
Circulation ; 143(7): 699-712, 2021 Feb 16.
Artigo em Inglês | MEDLINE | ID: mdl-33587660

RESUMO

BACKGROUND: The heart undergoes physiological hypertrophy during pregnancy in healthy individuals. Metabolic syndrome (MetS) is now prevalent in women of child-bearing age and might add risks of adverse cardiovascular events during pregnancy. The present study asks if cardiac remodeling during pregnancy in obese individuals with MetS is abnormal and whether this predisposes them to a higher risk for cardiovascular disorders. METHODS: The idea that MetS induces pathological cardiac remodeling during pregnancy was studied in a long-term (15 weeks) Western diet-feeding animal model that recapitulated features of human MetS. Pregnant female mice with Western diet (45% kcal fat)-induced MetS were compared with pregnant and nonpregnant females fed a control diet (10% kcal fat). RESULTS: Pregnant mice fed a Western diet had increased heart mass and exhibited key features of pathological hypertrophy, including fibrosis and upregulation of fetal genes associated with pathological hypertrophy. Hearts from pregnant animals with WD-induced MetS had a distinct gene expression profile that could underlie their pathological remodeling. Concurrently, pregnant female mice with MetS showed more severe cardiac hypertrophy and exacerbated cardiac dysfunction when challenged with angiotensin II/phenylephrine infusion after delivery. CONCLUSIONS: These results suggest that preexisting MetS could disrupt physiological hypertrophy during pregnancy to produce pathological cardiac remodeling that could predispose the heart to chronic disorders.

2.
J Hazard Mater ; 401: 123794, 2021 01 05.
Artigo em Inglês | MEDLINE | ID: mdl-33113737

RESUMO

In this work, an ultrasensitive and selective electrochemiluminescence (ECL) aptasensor with Au-tetrahedral aptamer nanostructure (Au-TAN) for acetamiprid detection was developed, which employed luminescence property of luminol and hydrogen peroxide (H2O2) as a co-reactant to apply the prepared Au-TAN to the luminescence systems. Au-TAN was prepared to modify an electrode surface via an Au-S bond to form a stable tetrahedral nanostructure. Fixed on the surface of the working electrode, Au-TAN could not only enhance the function of the aptamer but also boost the sensing performance. At the same time, Au nanoparticles (AuNPs) of the Au-TAN could also catalyze H2O2, thereby enhancing the luminescence performance of this aptasensor. The pH of the buffer solution, the concentration of H2O2 and the concentration of Au-TAN were optimized. Under the optimal conditions, the aptasensor had a detection limit of 0.0576 pM (S/N = 3), which was lower than those of other aptasensors for acetamiprid detection. Moreover, the weak alkaline environment explored in the experiment could expand its application range. Above all, the proposed method presented a high accuracy and sensitivity.

3.
Can J Physiol Pharmacol ; 98(7): 466-472, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32160476

RESUMO

The glucose intolerance developed during pregnancy is called gestational diabetes mellitus (GDM). GDM has become a severe risk for the health of both mother and baby. Astragaloside IV (AS-IV) is the dominant active component in Astragalus membranaceus and has been reported to have anti-inflammation and immune-regulation function. We aimed to demonstrate the function of AS-IV in the therapy of GDM and the molecular mechanism in this process. C57BL/KsJ-Lepdb/+ female mice were used as the GDM model. The mRNA levels of relative genes in this research were detected by quantitative real-time PCR. The protein levels of relative genes were analyzed by Western blot. Serum lipid level was measured with an ILab Chemistry Analyzer 300 PLUS. Glucose, insulin, and lipid profile levels in the GDM mice model were decreased by AS-IV treatment. AS-IV downregulated the expression of inflammatory genes and upregulated the expressions of anti-oxidant genes in the GDM mice model. AS-IV treatment reduced cAMP accumulation in liver and reduced hepatic gluconeogenesis in GDM mice. This study demonstrated that AS-IV treatment has an effective therapeutic function of GDM in a mice model through the regulation of cAMP accumulation and hepatic gluconeogenesis.

4.
Restor Neurol Neurosci ; 37(6): 571-581, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31796710

RESUMO

Many ocular diseases (such as glaucoma, diabetic retinopathy, age-related macular degeneration, and traumatic eye injuries) can result in the degeneration of retinal cells and the subsequent loss of vision. Some kinds of treatments, such as drugs, stem cell transplantation and surgery are reported to be effective in certain patients. However, no confirmatively effective, convenient and low-price intervention has been available so far. Physical exercise has been reported to exert neuroprotective effects on several neurodegenerative diseases, including Parkinson's disease and Alzheimer's disease. Studies investigating the potential impacts of exercise on retinal diseases are rapidly emerging. Here we review these up-to-date findings from both human and animal studies, and discuss the possible mechanisms underlying exercise-elicited protection on retina.


Assuntos
Exercício Físico/fisiologia , Neuroproteção/fisiologia , Condicionamento Físico Animal/fisiologia , Retina/fisiologia , Doenças Retinianas/prevenção & controle , Animais , Humanos , Doenças Neurodegenerativas/fisiopatologia , Doenças Neurodegenerativas/prevenção & controle , Estresse Oxidativo/fisiologia , Condicionamento Físico Animal/tendências , Doenças Retinianas/fisiopatologia
5.
Infect Drug Resist ; 12: 3653-3661, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31819548

RESUMO

Purpose: This study aimed to investigate the species distributions and drug sensitivities among 19 strains of Nocardia isolated from Yantai, China, from 2017 to 2019. Patients and methods: Definitive species identification was performed by sequencing a fragment of the 16S rRNA gene (1480 bp) and by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). The susceptibilities of the isolates to 15 commonly-used antibiotics were tested using the microbroth dilution method. Results: Among the 19 Nocardia isolates, five species were confirmed. Seventeen of the 19 Nocardia spp. strains were identified consistently by the two methods, while two isolates of N. cyriacigeorgica were misidentified as N. otitidiscaviarum by MALDI-TOF MS. N. farcinica was the most common species (8/19), followed by N. cyriacigeorgica (6/19), N. otitidiscaviarum (2/19), N. brasiliensis (2/19), and N. nova (1/19). All isolates were susceptible to trimethoprim-sulfamethoxazole and amikacin, followed by linezolid and tigecycline (94.7% susceptibility rates). The sensitivity and minimum inhibitory concentration patterns for ciprofloxacin, moxifloxacin, clarithromycin, and tobramycin were significantly correlated with the species. Conclusion: These results regarding the distribution and antibiotic resistance features of Nocardia species further our understanding of the diversity of Nocardia species circulating in Yantai, China, and thus support the use of more accurate empirical treatments.

6.
Reprod Biol Endocrinol ; 17(1): 77, 2019 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-31558153

RESUMO

BACKGROUND: As the most ordinary metabolic disorder during pregnancy, gestational diabetes mellitus (GDM) has become a severe risk for the health of both pregnant female and fetus. Astragaloside IV (AS-IV) is the dominant active component in Astragalus membranaceus. It has been proved that AS-IV has anti-inflammation and immune-regulation function. We aimed to demonstrate the function of AS-IV in the therapy of GDM and the molecular mechanism in this process. METHODS: C57BL/KsJ-Lepdb/+ female mice were used as GDM model. The mRNA levels of relative genes in this research were detected by qRT-PCR. The protein levels of relative genes were analyzed by western blot. Serum concentration of interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) were analyzed by ELISA. RESULTS: Glucose and insulin levels in GDM mice model were decreased by AS-IV treatment. AS-IV down-regulated the expression of inflammatory gene IL-6 and TNF-α in GDM mice model. AS-IV treatment inhibited the expression of NLR family pyrin domain containing-3 (NLRP3) inflammasome relative proteins in the pancreas of GDM mice. CONCLUSION: This study demonstrated that AS-IV treatment has an effective therapeutic function of GDM in mice model through the inhibition of NLRP3 inflammasome in the pancreas.


Assuntos
Diabetes Gestacional/tratamento farmacológico , Inflamassomos/efeitos dos fármacos , Proteína 3 que Contém Domínio de Pirina da Família NLR/genética , Saponinas/farmacologia , Triterpenos/farmacologia , Animais , Glicemia/metabolismo , Diabetes Gestacional/genética , Diabetes Gestacional/metabolismo , Feminino , Regulação da Expressão Gênica/efeitos dos fármacos , Inflamassomos/genética , Inflamassomos/metabolismo , Insulina/sangue , Interleucina-6/sangue , Interleucina-6/genética , Interleucina-6/metabolismo , Camundongos Endogâmicos C57BL , Estrutura Molecular , Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo , Gravidez , Saponinas/química , Triterpenos/química , Fator de Necrose Tumoral alfa/sangue , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/metabolismo
7.
Clin Transl Sci ; 12(1): 53-57, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30548202

RESUMO

This study was designed to establish a modified prediction score system to improve the safety and success rate of vaginal birth after cesarean delivery (VBAC). We recruited 406 patients (between January 2012 and December 2016) and generated a modified score system in predicting the success rate of VBAC. All patients were required to sign informed consent forms. There were 87.2% of patients who had successful VBAC deliveries and 12.8% patients who had repeated cesarean sections. We conducted multivariable logistic regression and found seven variables that were associated with VBAC success, including previous primary indication of cesarean delivery (odds ratio (OR), 2.1; 95% confidence interval (CI), 1.4-3.0), previous vaginal birth history (OR, 2.5; 95% CI, 1.8-3.8), < 40 years of age (OR, 2.1; 95% CI, 1.2-3.3), < 20 kg weight gain (OR, 1.5; 95% CI, 1.2-2.3), no labor induction (OR, 1.9; 95% CI, 1.5-2.9), high score of pelvic/birth weight (OR, 1.4; 95% CI, 1.1-2.1), and Bishop score (OR, 1.3; 95% CI, 1.2-1.4). After adjustment for optimism, the area under the receiver operating characteristic curve (AUC-ROC) was 0.849 (95% CI, 0.78-0.89), and the modified VBAC score was positively correlated with the success rate of trial of labor after cesarean delivery (TOLAC). A valid and useful score system was established to predict VBAC success rate.


Assuntos
Cesárea/efeitos adversos , Modelos Biológicos , Prova de Trabalho de Parto , Nascimento Vaginal Após Cesárea/estatística & dados numéricos , Adulto , Fatores Etários , Feminino , Previsões/métodos , Ganho de Peso na Gestação , Humanos , Modelos Logísticos , Razão de Chances , Gravidez , Curva ROC , Estudos Retrospectivos , Adulto Jovem
8.
Reprod Health ; 15(1): 84, 2018 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-29793556

RESUMO

BACKGROUND: The goal was to study whether higher physical activity can increase the success rate of Vaginal Birth after Cesarean Delivery (VBAC). METHODS: We enrolled 823 patients with previous cesarean section delivery history (between January 2015 and December 2017) and measured their physical activity during pregnancy. A final number of 519 patients were included for the trial of labor after cesarean delivery (TOLAC). All patients signed informed consent forms. RESULTS: We conducted bivariate analyses and identified that several variables were associated with successful VBAC: Prior history of vaginal birth (odds ratio [OR] 2.4, 95% CI 1.8-3.9); previous indication for primary cesarean delivery (OR 2.2, 95% CI 1.5-3.0); age younger than 40 years (OR 2.1, 95% CI 1.3-3.4); Weight gain less than 20 kg (OR 1.5, 95% CI 1.3-2.4); high pelvic/birth weight score (OR 1.4, 95% CI 1.1-2.0); no induction of labor (OR 1.9, 95% CI 1.4-2.8); and estimated prenatal fetal weight (OR 1.4, 95% CI 1.2-1.5). We also found that the bivariate association between physical activity and VBAC was significant (p = 0.002). In addition, there was higher odds of VBAC in women who had active physical activity of more than 150 min/week (adjusted OR 1.86, 95% CI 1.69-2.07). Lower odds of VBAC was associated with older age, weight gain during pregnancy, induction of labor, and having estimated prenatal fetal weight more than 3500 g. CONCLUSION: Physical activity during pregnancy may influence the success rate of VBAC in Chinese women. Future studies will be needed to prove the robustness of this association using more detailed exposure and outcome definitions.


Assuntos
Terapia por Exercício , Complicações do Trabalho de Parto/prevenção & controle , Prova de Trabalho de Parto , Nascimento Vaginal Após Cesárea/estatística & dados numéricos , Adulto , Peso ao Nascer , Feminino , Idade Gestacional , Humanos , Gravidez , Resultado da Gravidez , Ganho de Peso
9.
J Pharmacol Sci ; 131(2): 93-100, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27156928

RESUMO

Gestational diabetes mellitus (GDM) is a disease commonly occurs during mid to late pregnancy with pathologies such as hyperglycemia, hyperinsulinemia and mal-development of fetus. We have previously demonstrated that pancreatic endoderm (PE) derived from human embryonic stem cells (hESCs) effectively alleviated diabetic symptoms in a mouse model of GDM, although the clinical efficacy was limited due to oxidative stress. In this study, using the anti-oxidant agent naringenin, we aimed to further enhance the efficacy of hESC-derived PE transplant. Insulin-secreting PE was differentiated from hESCs, which were then transplanted into GDM mice. Naringenin was administered to mice receiving the PE transplant, with sham operated mice serving as negative control, to assess its effect on alleviation of GDM symptoms. We found that naringenin supplement further improved insulin response, glucose metabolism and reproductive outcome of the PE-transplanted female mice. Our new findings further potentiates the feasibility of using differentiated hESCs to treat GDM, in which anti-oxidative agent such as naringenin could greatly enhance the clinical efficacy of stem cell based therapies.


Assuntos
Antioxidantes/uso terapêutico , Diabetes Gestacional/terapia , Endoderma/transplante , Flavanonas/uso terapêutico , Animais , Antioxidantes/farmacologia , Glicemia/análise , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Diabetes Gestacional/sangue , Diabetes Gestacional/metabolismo , Feminino , Flavanonas/farmacologia , Células-Tronco Embrionárias Humanas/efeitos dos fármacos , Humanos , Peróxido de Hidrogênio/farmacologia , Insulina/sangue , Insulina/metabolismo , Secreção de Insulina , Tamanho da Ninhada de Vivíparos , Camundongos , Oxidantes/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Pâncreas , Gravidez , Espécies Reativas de Oxigênio/metabolismo
10.
Neuroreport ; 27(6): 435-44, 2016 Apr 13.
Artigo em Inglês | MEDLINE | ID: mdl-26966780

RESUMO

This study was designed to clarify the neuroprotective effects of tanshinone IIA (TSA) following cerebral ischemic insult. Adult Sprague-Dawley rats were operated upon to achieve a middle cerebral artery occlusion to cause transient focal cerebral ischemia, which were then randomly divided into the sham-operated control group and cerebral ischemia/reperfusion (I/R) groups receiving a 2 h occlusion. The treatment groups received daily intraperitoneal injections of high or low doses of TSA, for 7 or 15 days. NeuN immunostaining revealed neuronal loss following I/R, which was partially prevented with subsequent TSA dosing. Protein disulfide isomerase and adenosine triphosphatase (Na(+)/K(+)-ATPase) levels were all depressed by means of I/R. TSA treatment markedly reversed the depression of all indices examined. The intensity of microglial activation, as evidenced with CD11b staining, was increased by means of cerebral artery occlusion, but this was partially reversed with subsequent TSA treatment. TSA may affect neuroprotection by way of minimizing deficits in energy metabolism and reduction of the extent of cell death within affected regions.


Assuntos
Abietanos/uso terapêutico , Anti-Inflamatórios não Esteroides/uso terapêutico , Infarto da Artéria Cerebral Média/tratamento farmacológico , Fosfopiruvato Hidratase/metabolismo , Isomerases de Dissulfetos de Proteínas/metabolismo , ATPase Trocadora de Sódio-Potássio/metabolismo , Animais , Encéfalo/patologia , Antígeno CD11b/metabolismo , Modelos Animais de Doenças , Infarto da Artéria Cerebral Média/complicações , Infarto da Artéria Cerebral Média/patologia , Masculino , Doenças do Sistema Nervoso/tratamento farmacológico , Doenças do Sistema Nervoso/etiologia , Ratos , Ratos Sprague-Dawley
11.
Brain Res ; 1546: 1-8, 2014 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-24365206

RESUMO

The ability of melatonin treatment of aged animals to partially restore the pattern of gene expression characterizing the younger animal has been frequently reported. The current study examines the effect of melatonin upon age-related changes of some key proteins relevant to the aging process. Male B6C3F1 mice, aged 5.5 months and 23.4 months were used as a model for aging and half of each group received a diet supplemented with 40-ppm (w/w) melatonin for 9.3 weeks. Protein components of the globus pallidus were studied including glial fibrillary acidic protein (GFAP), NF-κB, protein disulfide isomerase (PDI), and Nissl staining. Some age-related changes were in an upward direction (GFAP and NF-κB), while others were depressed with age (PDI and intensity of Nissl staining). However, in either case, melatonin treatment of aged mice generally altered these parameters so that they came to more closely resemble the levels found in younger animals. The extent of this reversal to a more youthful profile, ranged from complete (for NF-κB) to very minor (for Nissl staining and PDI). Overall, these findings are in accord with prior data on the effect of melatonin on cortical gene expression and confirm the value of melatonin as a means of retarding events associated with senescence.


Assuntos
Envelhecimento/efeitos dos fármacos , Suplementos Nutricionais , Globo Pálido/efeitos dos fármacos , Melatonina/farmacologia , Envelhecimento/metabolismo , Animais , Proteína Glial Fibrilar Ácida/metabolismo , Globo Pálido/citologia , Globo Pálido/metabolismo , Masculino , Melatonina/administração & dosagem , Camundongos , NF-kappa B/metabolismo , Isomerases de Dissulfetos de Proteínas/metabolismo
12.
Neurochem Res ; 39(2): 295-304, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24362639

RESUMO

Acute inflammation plays an important role in brain damage following cerebral ischemia and reperfusion (I/R) injury. The present study employed a rat model of middle cerebral artery occlusion to explore the neuroprotective effects of tanshinone IIA (TSN), which is widely used in China for treating cerebrovascular and cardiovascular diseases. Rats were divided into a sham-operated group and I/R transiently occluded then reperfused groups. Some of the I/R animals were treated daily for 7 or 15 days with two different doses of TSN. After 15 days, triphenyl tetrazolium chloride staining revealed less unstained area indicating fewer lesions in the TSN-treated I/R group relative to the untreated corresponding I/R group. TSN treatment dramatically reduced infarct sizes and reduced content of high mobility group box 1 protein following I/R. Nuclear translocation of NFκB was also attenuated in I/R animals subsequently receiving TSN. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling staining revealed more apoptosis in the I/R model group and this was reduced in the I/R animals treated with TSN for 15 days. Thus, TSN mitigates the severity of damage effected by I/R.


Assuntos
Abietanos/farmacologia , Infarto Cerebral/prevenção & controle , Proteína Glial Fibrilar Ácida/metabolismo , Proteína HMGB1/metabolismo , Infarto da Artéria Cerebral Média/metabolismo , NF-kappa B/metabolismo , Fármacos Neuroprotetores/farmacologia , Animais , Western Blotting , Infarto Cerebral/metabolismo , Infarto Cerebral/patologia , Marcação In Situ das Extremidades Cortadas , Masculino , Ratos , Ratos Sprague-Dawley
13.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 29(9): 897-900, 2013 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-24011146

RESUMO

OBJECTIVE: To observe the expression levels of the complement fragment C1q and C3c in rat brain tissues with cerebral ischemia-reperfusion (I/R) injury, and explore the correlation, roles and mechanism of complement reaction and microglia in the brain I/R injury. METHODS: A total of 48 male Sprague-Dawley rats were randomly divided into normal control group, sham group, I/R 24 h, 72 h, 7 d, 15 d model groups. Suture occlusion method was operated to establish focal middle cerebral artery occlusion (MCAO) and reperfusion models. The Nissl staining was applied to observe the structure of neurons, and immunohistochemistry was applied to detect CD11b, C1q and C3c expression. RESULTS: Compared with the sham group, Nissl staining reaction in brain tissues was stronger in the I/R 24 h group, and then became weaker, and the reduction was the most significant in the I/R 72 h group. The expression of CD11b protein increased in the I/R 24 h group and reached the peak value in the I/R 72 h group, followed by gradually reducing. Compared with the sham group, all the model groups were significantly stronger in CD11b expression (P<0.05). C1q and C3c sharply increased in the brain tissue of I/R 24 h group and peaked in the I/R 7 d group, and then presented a downward trend; the differences between the sham group and all the model groups were of statistical significance (P<0.05). CONCLUSION: The expression levels of C1q and C3c are positively correlated with CD11b protein in rat brain tissues with cerebral I/R injury, suggesting that cerebral I/R injury inintiate the brain innate immune response, activates complement C1q and C3c as well as microglia, thus playing the role of protection or damage in cerebral I/R injury.


Assuntos
Isquemia Encefálica/imunologia , Encéfalo/imunologia , Complemento C1q/fisiologia , Complemento C3c/fisiologia , Traumatismo por Reperfusão/imunologia , Animais , Antígeno CD11b/análise , Complemento C1q/análise , Complemento C3c/análise , Masculino , Ratos , Ratos Sprague-Dawley
14.
Zhonghua Xin Xue Guan Bing Za Zhi ; 40(8): 684-9, 2012 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-23141015

RESUMO

OBJECTIVE: To observe the histopathological features, nuclear factor-κB (NFκB) and IKB expressions as well as calcium deposition of atherosclerosis plaques (AS) in apolipoprotein E (ApoE) and low density lipoprotein receptor (LDLR) knockout mice (ApoE(-/-), LDLR(-/-)fed high-fat diet. METHODS: Eight C57BL/6J mice fed with normal diet were used as control, 32 ApoE(-/-) mice and LDLR(-/-) mice were divided into normal diet and high-fat diet groups (n = 8 each). After 4 months, aorta was collected for morphologic (HE, Oil Red O, Von Kossa) and immunohistochemistry (nuclear factor-κB, IKB, macrophage surface molecule-3, α-smooth action protein) analysis. RESULTS: Degree of AS in ApoE(-/-) and LDLR(-/-) mice fed with high-fat diet were significantly severer than those fed with normal diet and AS was more significant in ApoE(-/-) mice than in LDLR(-/-) mice. NFκB and IKB expressions in high-fat diet group were significantly higher than the normal diet group (P < 0.05). Double-labeling of NFκB revealed dominant expression in smooth muscle cells. Calcium deposition was significantly more in ApoE(-/-) mice fed with high-fat diet than mice fed with normal diet (P < 0.05) and was similar in LDLR(-/-) mice fed with high and normal diet (P > 0.05). CONCLUSION: High-fat diet contributes to the formation of AS plagues in ApoE(-/-) and LDLR(-/-) mice joined by upregulated NFκB and IKB expressions and calcium deposition.


Assuntos
Apolipoproteínas E/metabolismo , Proteínas I-kappa B/metabolismo , NF-kappa B/metabolismo , Placa Aterosclerótica/metabolismo , Receptores de LDL/metabolismo , Animais , Apolipoproteínas E/genética , Cálcio/metabolismo , Feminino , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Placa Aterosclerótica/patologia , Receptores de LDL/genética
15.
Zhong Yao Cai ; 35(10): 1628-32, 2012 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-23627131

RESUMO

OBJECTIVE: To observe the neuroprotective effect of tanshinone II(A) (Tan II(A)) on the expression of brain tissue glial fibrillary acidic protein (GFAP) and adenosine triphosphatase (ATPase) and protein disulfide isomerase (PDI) of cerebral ischemia reperfusion (I/R) injury of different time in rats, and investigate the neuroprotective and its molecular mechanism of Tan II(A) on brain injury. METHODS: Sixty-four male Sprague-Dawley rats were randomly devided into eight groups (n = 8 per group): Group 1, sham-operated animals without I/R; Group 2, animals with I/R of 3 days; Group 3, animals with I/R of 7 days; Group 4, animals with I/R of 7 days and treatment with low doses of Tan II(A); Group 5, animals with IR of 7 days, treated with high doses of Tan II(A); Group 6, animals with I/R of 15 days; Group 7, animals with IR of 15 days and low doses of Tan II(A) treatment; Group 8, animals with I/R of 15 days, treated with high doses of Tan II(A). The model of focal middle cerebral artery occlusion (MCAO) was established by suture-occluded method. After Tan II(A) treatment, pathological changes of brain tissue in all groups were observed by hematoxylin-eosin staining (HE) and the expression levels of GFAP, ATP and PDI by immunohistochemistry staining. RESULTS: (1) The pathological changes of ischemic injury in low and high dose of Tan II(A) treatment groups were lighter than those in I/R groups, and so were in high dose of Tan II(A) treatment group than in low dose Tan II(A) treatment group. (2) Compared with sham-operated group, expression levels of GFAP in the three different I/R groups increased evidently, while the levels in high dose of Tan II(A) treatment groups were relatively low (P < 0.05). There was no statistically difference between high dose of Tan II(A) treatment group and low dose of Tan II(A) treatment group in either 7 or 15 days treatment groups (P > 0.05). (3) Compared with sham-operated group, expression levels of ATPase and PDI in the three different I/R groups all decreased clearly; Compared with I/R groups, expression levels of ATPase and PDI in Tan II(A) treatment groups increased in the ischemic territory obviously (P < 0.05). CONCLUSIONS: Tan II(A) may have a neuroprotective effect on ischemia-reperfusion injury by inhibiting the production of GFAP to reduce the excessive inflammatory response produced by glial cells in brain and up-regulating the activities of ATPase and PDI in neurons to improve the balance of energy metabolism and maintain the intracellular homeostasis.


Assuntos
Abietanos/farmacologia , Adenosina Trifosfatases/metabolismo , Isquemia Encefálica/metabolismo , Proteína Glial Fibrilar Ácida/metabolismo , Traumatismo por Reperfusão/metabolismo , Salvia miltiorrhiza/química , Abietanos/administração & dosagem , Abietanos/uso terapêutico , Animais , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Encéfalo/patologia , Isquemia Encefálica/tratamento farmacológico , Isquemia Encefálica/patologia , Modelos Animais de Doenças , Imuno-Histoquímica , Infarto da Artéria Cerebral Média , Masculino , Fármacos Neuroprotetores/administração & dosagem , Fármacos Neuroprotetores/farmacologia , Fármacos Neuroprotetores/uso terapêutico , Isomerases de Dissulfetos de Proteínas/metabolismo , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Traumatismo por Reperfusão/prevenção & controle , Rizoma/química
16.
Chin J Integr Med ; 12(1): 6-11, 2006 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-16571276

RESUMO

OBJECTIVE: To assess the efficacy and safety of Zhongyan-4 (ZY-4, a Chinese herbal preparation worked out according to the therapeutic principle of supplementing qi, nourishing Yin, clearing heat and detoxication) in treating HIV/AIDS patients in the early or middle stage. METHODS: Adopted was randomized double-blinded and placebo-parallel-controlled method, with 72 HIV/AIDS patients randomly divided into the ZY-4 group (36 patients) treated with ZY-4 and the control group (36 patients) treated with placebo. The treatment course was six months. The index of CD(4)(+), CD(8)(+) counts, body weight, clinical symptom scoring were estimated at 4 time points (0, 1, 3 and 6 month in the course), and also the viral load before and after treatment. The whole course of observation was completed in 63 patients, 30 in the ZY-4 group and 33 in the control group. RESULTS: CD(4)(+) count in the ZY-4 group got elevated by 7.70 +/- 150.96/mm(3) on average, while that in the control group lowered by 27.33 +/- 85.28/mm(3). Fifteen out of the 30 patients in the ZY-4 group had their CD(4)(+) count increased, which was evidently much higher than that in the control group (8/33, P < 0.05), suggesting that the efficacy of ZY-4 is superior to that of placebo in elevating CD(4)(+) count. Moreover, ZY-4 showed actions in elevating CD(45)RA(+) and CD(8)(+) count, reducing HIV virus load, improving clinical symptom/sign and increasing body weight of patients. No obvious adverse reaction was found in the clinical trial. CONCLUSION: ZY-4 has an immunity-protective and/or rebuilding function in HIV/AIDS patients in the early and middle stage, and also shows effects in lowering viral load, increasing body weight and improving symptoms and signs to a certain degree.


Assuntos
Síndrome de Imunodeficiência Adquirida/tratamento farmacológico , Fármacos Anti-HIV/uso terapêutico , Medicamentos de Ervas Chinesas/uso terapêutico , Infecções por HIV/tratamento farmacológico , Fitoterapia , Síndrome de Imunodeficiência Adquirida/imunologia , Síndrome de Imunodeficiência Adquirida/virologia , Adulto , Fármacos Anti-HIV/efeitos adversos , Peso Corporal , Contagem de Linfócito CD4 , Relação CD4-CD8 , Método Duplo-Cego , Medicamentos de Ervas Chinesas/efeitos adversos , Feminino , Infecções por HIV/imunologia , Infecções por HIV/virologia , Humanos , Antígenos Comuns de Leucócito/análise , Masculino , Pessoa de Meia-Idade , Carga Viral
17.
Zhonghua Yi Xue Za Zhi ; 85(41): 2926-9, 2005 Nov 02.
Artigo em Chinês | MEDLINE | ID: mdl-16324367

RESUMO

OBJECTIVE: To investigate the cognitive function and psychological characteristics of the patients with chronic fatigue syndrome (CFS) in China and analyze its relation with primary psychological diseases. METHODS: Ninety-one patients with CFS who visited the People's Hospital, Peking University, in Beijing from Beijing, Shanghai, Tianjin, Heilongjiang, Jilin, Hebei, Inner Mongolia, Shanxi, Shandong, Sichuan, Gansu, Fujian, and Guangdong, 42 males and 49 females, aged 37 +/- 7, 43% of which had the record of formal schooling of regular college course or over and 21 of which had the record of formal schooling of college for professional training, and 58% of which showed clear causes, diagnosed by the CDC criteria 1994, underwent case history collection, physical examination, necessary laboratory test, memory test, and SCL-90, Hamilton depression rating scale (HAMD), and Hamilton anxiety rating scale (HAMA) testing. Thirty healthy persons, 14 males and 16 females, aged 37 +/- 7, were used as controls., A table of case file was established based on the CDC criteria 1994 for each patient to record the relevant data. Independent-Samples T Test was used to compare the memory quotient, the total score and general mean score of SCL-90, the score of HAMD and HAMA. Analyzed the impairment of cognitive function and psychological characteristics of patients with CFS. RESULTS: The most common symptoms was descent of remembrance and/or attention (82/91, 90%). The memory quotient of the CFS patients was 85 +/- 14, significantly lower than that of the healthy controls (98 +/- 12, t = 4.627, P = 0.000). The total score of SCL-90 of the CFS patients was 192 +/- 47, significantly higher than that of the healthy controls (140 +/- 46, t = 5.297, P = 0.000). The symptoms with a factor score > or = 2.0 in SCL-90 included obsessive-compulsive symptoms (61/91, 67%), somatization (61/91, 67 %), depression (57/91, 63%), and anxiety (49/91, 54%). The HAMD score of the CFS patients was 9.9 +/- 6.1, significantly higher than that of the healthy controls (6.5 +/- 2.5, t = 2.948, P = 0.004). The HAMA score of the CFS patients was 9.9 +/- 7.0, significantly higher than that of the healthy controls (5.9 +/- 2.9, t = 3.015, P = 0.003). CONCLUSION: The CFS patients in China have an obvious impairment of remembrance and show different psychological abnormalities that are different from those of the patients with primary psychological diseases.


Assuntos
Cognição , Síndrome de Fadiga Crônica/psicologia , Adulto , Depressão , Síndrome de Fadiga Crônica/complicações , Feminino , Humanos , Masculino , Memória , Pessoa de Meia-Idade
18.
Guang Pu Xue Yu Guang Pu Fen Xi ; 24(2): 145-8, 2004 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-15769001

RESUMO

A new chemiluminescence-based method was proposed for selective quantitation of analgin in pharmaceutical preparation. The CL emission was generated by mixing analgin, polyethylene glycol-400 and rhodamine 6G in acidic medium. The analgin content could be determined by the CL intensity. No oxidants were used in the system, so it was difficult to explain why the CL emission was produced. The mechanism of CL emission remains under investigation. One of the advantages of this method is highly selective. No other pharmaceutical compounds and chemicals could produce CL emission whether they contain sulfuric group or not in the experiment. There was no disturbance in this method, which was different from others. Another advantage is high sensitivity. The quantitation range is from 0.01 to 10 microg x mL(-1) with a detection limit of 0.003 microg x mL(-1) for analgin. This is the lowest detection limit of any CL methods currently available for analgin determination. The proposed method was applied to the selective quantitation of analgin in pharmaceutical preparation.


Assuntos
Dipirona/análise , Análise de Injeção de Fluxo/métodos , Medições Luminescentes/métodos , Dipirona/química , Luminescência , Preparações Farmacêuticas , Polietilenoglicóis/química , Rodaminas/química
19.
J Pharm Biomed Anal ; 30(3): 473-8, 2002 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-12367671

RESUMO

Strong chemiluminescence emission has been observed by mixing alkaline hydrolytic products of ethamsylate with Tween 80 in acidic rhodamine 6G solution. This phenomenon has been utilized to design a flow-injection chemiluminescence method for the determination of ethamsylate in a pharmaceutical preparation. Under the optimum conditions, the proposed procedure has a linear range between 0.05 and 2.0 microg ml(-1), with a detection limit of 0.02 microg ml(-1) for ethamsylate. The method was applied to the determination of ethamsylate in pharmaceutical preparations. The possible mechanism of this chemiluminescence reaction was proposed.


Assuntos
Etamsilato/análise , Preparações Farmacêuticas/análise , Etamsilato/química , Etamsilato/metabolismo , Medições Luminescentes , Oxirredução , Preparações Farmacêuticas/química , Preparações Farmacêuticas/metabolismo
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