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1.
Can J Microbiol ; : 1-15, 2020 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-31944857

RESUMO

Rhizosphere bacteria are key determinants of plant health and productivity. In this study, we used PCR-based next-generation sequencing to reveal the diversity and community composition of bacteria in the cotton rhizosphere from samples collected in Xinjiang Province, China. We identified 125 bacterial classes within 49 phyla from these samples. Proteobacteria (33.07% of total sequences), Acidobacteria (19.88%), and Gemmatimonadetes (11.19%) dominated the bacterial community. Marked differences were evident in the α-diversity of rhizosphere bacteria during different cotton plant growth and development stages. The operational taxonomic unit (OTU) numbers were highest in seedling and bud stages and decreased at the flowering and fruit-boll-opening stages. Forty-three OTUs from the Proteobacteria were common to all four periods of cotton development. Proteobacteria were more abundant in the rhizospheres of cotton from southern Xinjiang than from northern Xinjiang, while the opposite trend was observed for Acidobacteria. Gemmatimonadetes frequency was broadly the same in both northern and southern Xinjiang. These results suggest that there is abundant diversity in the microbiota of cotton rhizosphere soil. Proteobacteria and Actinobacteria dominated this microbial niche and bacterial communities in the seedling, bud, flowering, and boll-opening stages appear to be more similar to one another than to communities at the other growth stages.

2.
Shanghai Kou Qiang Yi Xue ; 28(1): 41-47, 2019 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-31080998

RESUMO

PURPOSE: The aim of this clinical study was to evaluate the changes of alveolar bone morphology around the upper incisors before and after functional treatment in adolescents using cone-beam CT(CBCT). METHODS: Thirty patients with skeletal Class II mandibular retrusion who were successfully treated with high-pull headgear-activator(HGAC) and Twin-block were selected and divided into 2 groups (HGAC and Twin-block groups), 15 in each group. CBCT was performed before and after treatment, to observe upper incisor movement in the alveolar bone and alveolar bone remodeling. Statistical analysis was performed using SPSS 20.0 software package to analyze the changes of alveolar bone thickness, angle of central incisor and alveolar bone before and after treatment. RESULTS: Horizontally, the edge of the maxillary incisor appeared lingual movement in both groups, while the root apex appeared lingual movement in HGAC group and labial movement in Twin-block group. Vertically, the edge of the maxillary incisor was moved down and the root apex was moved up in all patients, whereas the moving distance was less in the edge and larger in the root apex in HGAC group. The thicknesses of major areas in the alveolar bone significantly increased in HGAC group, while in Twin-block group the labial thickness of the alveolar bone showed significant decrease and the palatal thickness showed significant increase. Moreover, the total thickness of the alveolar bone showed significant increase in both groups, yet Twin-block group showed more increase, and the angle of the alveolar bone showed more decrease in HGAC group. CONCLUSIONS: Both functional appliances can cause positive alveolar bone remodeling in maxillary incisor area. HGAC can achieve a controlled tilt inward movement of the maxillary incisors, intrude the incisors to a certain extent, and allow certain change in the bending angle of the incisor alveolar bone at the same time, which is conducive to improving ClassⅡcraniofacial pattern. Twin-block can tilt the maxillary incisor inward, suggesting that more attention needs to be paid to the control of the torque of the incisor when retracting anterior teeth in fixed orthodontic treatment after Twin-block functional treatment.


Assuntos
Remodelação Óssea , Incisivo , Maxila , Adolescente , Tomografia Computadorizada de Feixe Cônico , Aparelhos de Tração Extrabucal , Humanos
3.
Cell Death Dis ; 10(3): 159, 2019 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-30770793

RESUMO

Notwithstanding the researches on biomarkers and targeted therapies in renal cell carcinomas (RCC) have made progress in the last decades, the application of the biomarkers and targeted therapy agents for RCC in clinic are restricted because of their limitation or side effects. Liver X receptors (LXRs) and the NLRP3 inflammasome have been the research hotspots in recent years. In our study, we integrated bioinformatics analysis, molecular biology experiments and biological function experiments to study the roles of LXRα and the NLRP3 inflammasome in RCC. The study demonstrated that the elevated LXRα expression is correlated with a poor prognosis in RCC. Furthermore, our study revealed the expression levels and roles of the NLRP3 inflammasome in RCC for the first time. This research demonstrated that LXRα could promote the metastasis of RCC cells by suppressing the expression of the NLRP3 inflammasome. In Brief, LXRα had the possibility to be a novel diagnostic and prognostic biomarker and therapeutic target in renal cell cancer and LXRα could regulate the metastasis of renal cell cancer via NLRP3 inflammamsome.

4.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 49(5): 797-802, 2018 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-30378345

RESUMO

OBJECTIVE: To determine factors associated with adverse outcomes of preeclampsia and develop a predictive model. METHODS: Clinical data of 2 532 patients with preeclampsia who were admitted to our hospital from 2005 to 2014 were extracted for the study. The patients were divided into two groups, including 990 (39.1%) with adverse outcomes and 1 542 (60.9%) without adverse outcomes. Factors associated with adverse outcomes were identified through univariate analyses. The predictive model was developed through multivariate logistic regression analyses using a randomly selected sample containing 80% of the cases. The remaining 20% of cases served for the purpose of validation and the establishment of the ROC curve. RESULTS: Primiparas, educational attainments, prenatal care, multiple births, edema, chest pain, dyspnea, dizziness, headache, blurred vision, intrahepatic cholestasis of pregnancy, gestational diabetes, cardiovascular disease, blood pressure, urine protein, liver and kidney functions were found to be associated with adverse outcomes of preeclampsia. Multiple births, edema, dyspnea, blurred vision, cardiovascular disease, liver and kidney functions entered into the logistic regression model (P<0.05). The Logit(P) model had a good fitness of data and 77.1% accuracy in predicting adverse outcomes. The area under the curve (AUC) of the ROC curve was 0.804 [P<0.01, 95% confidence interval CI): 0.758 to 0.849]. The highest sensitivity was achieved when the cut-off point set risk value at 0.300, [CM(155mm]with 58.6% patients having adverse outcomes representing 83.8% true positive rate and 46.8% false positive rate. CONCLUSION: Adverse outcomes of preeclampsia can be predicted through multiple births, edema, dyspnea, blurred vision, cardiovascular disease, liver and kidney functions. Risk value ≥0.300 is recommended.


Assuntos
Pré-Eclâmpsia/diagnóstico , Resultado da Gravidez , Área Sob a Curva , Feminino , Humanos , Modelos Logísticos , Gravidez , Curva ROC , Fatores de Risco
5.
Chin Med J (Engl) ; 131(21): 2566-2574, 2018 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-30381590

RESUMO

Background: The effect of maternal weights on the risk of iron deficiency anemia (IDA) during pregnancy remains unclear. The study aimed to investigate the association between maternal weight indicators and IDA during pregnancy. Methods: We conducted a cohort study to examine the association between maternal weight indicators, including prepregnancy body mass index and the rate of gestational weight gain (GWG), and the risk of IDA among Chinese pregnant women. Data about new-onset IDA at different trimesters from a national cross-sectional survey were collected; information regarding baseline variables and rate of GWG from women participating in the survey were retrospectively collected. Tested IDA and reported IDA were documented. Multilevel logistic regression to examine the association between maternal weight indicators and the risk of IDA after adjusting for potential confounders was conducted. Results: This study enrolled 11,782 pregnant women from 24 hospitals from September 19, 2016, to November 20, 2016. Among those, 1515 (12.9%) IDA events were diagnosed through test (test IDA); 3915 (33.3%) were identified through test and patient reporting (composite IDA). After adjusting for confounders and cluster effect of hospitals, underweight pregnant women, compared with normal women, were associated with higher risk of test IDA (adjusted odds ratio [aOR]: 1.35, 95% confidence interval [CI]: 1.17-1.57 and composite IDA (aOR: 1.35, 95% CI: 1.21-1.51); on the contrary, overweight and obese women had lower risk of test IDA (aOR: 0.68, 95% CI: 0.54-0.86 overweight; aOR: 0.30, 95% CI: 0.13-0.69 obese) and composite IDA (aOR: 0.77, 95% CI: 0.67-0.90 overweight; aOR: 0.34, 95% CI: 0.21-0.55 obese). The higher rate of GWG was associated with higher risk of IDA (test aOR: 1.86 95% CI: 1.26-2.76; composite aOR: 1.54, 95% CI: 1.16-2.03). Conclusions: Pregnant women who are underweight before pregnancy and who have faster GWG are more likely to develop IDA. Enforced weight control during pregnancy and use of iron supplements, particularly among underweight women, may be warranted.


Assuntos
Anemia Ferropriva/metabolismo , Anemia Ferropriva/patologia , Adulto , Índice de Massa Corporal , Estudos de Coortes , Estudos Transversais , Feminino , Ganho de Peso na Gestação/fisiologia , Humanos , Gravidez , Estudos Retrospectivos
6.
Front Pharmacol ; 9: 615, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29962947

RESUMO

Bleomycin (BLM) is a broad spectrum anti-tumor drug and inducing pulmonary fibrosis. As an anti-tumor drug without immunosuppression, it is urgent to find a drug that reduces the side effects of BLM. Scutellarin (SCU), a flavone extracted from Erigeron breviscapus (Vant.) Hand-Mazz, has anti-inflammatory activity and ability to inhibit tumor cell growth, migration, and invasion. However, the combined role of SCU and BLM treatment in tumor is unclear. This study aimed to investigate the possible effect and related mechanisms of BLM combined with SCU in the treatment of tumor through in vivo and in vitro experiments. In vivo experiments showed that BLM combined with SCU in the treatment of mice bearing H22 ascites tumor prolonged the survival time, alleviated BLM-induced pulmonary fibrosis, reduced the production of TNF-α; IL-6, and the levels of MDA and MPO. BLM combined with SCU increased the apoptotic rate of H22 ascites cells and the levels of cleaved-caspases-3 and -8. Furthermore, BLM combined with SCU increased the protein expression of p53 and gene expression of miR-29b, and decreased the expression of TGF-ß1. In vitro experiment results showed that BLM combined with SCU inhibited the viability of H22 cells and MRC-5 cells, promoted H22 cell apoptosis, up-regulated the protein expression of p53 and down-regulated the protein expression of α-SMA and collagen-I in MRC-5 cells. These experimental results suggested that SCU could enhance the anti-tumor effect of BLM and reduce BLM-induced pulmonary fibrosis, indicating SCU as a potential adjuvant for BLM in the future.

7.
Int Immunopharmacol ; 62: 15-22, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29966943

RESUMO

Pogostone (PO), a major component of Pogostemon cablin, displays potent protective effects against lipopolysaccharide-induced acute lung injury (ALI) in mice. This study aimed to investigate the protective effect of PO on TNF-α-induced cell injury in human alveolar epithelial cells in vitro and its underlying mechanism. The cell viability was measured using the MTS method. The cell apoptosis was determined using flow cytometry. The activities of reactive oxygen species (ROS) were detected using a fluorescence microscope. The pro-inflammatory cytokines and antioxidant genes were assessed using reverse transcription-polymerase chain reaction. The protein expression of Kelch-like ECH-associated protein 1 (Keap1), nuclear factor erythroid 2-related factor 2 (Nrf2), nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-alpha (IκBα), and nuclear factor-kappa B (NF-κB) p65 was analyzed using the Western blot analysis. PO alleviated cell apoptosis and inhibited ROS production. It alleviated TNF-α-induced cell injury, suppressed the levels of inflammatory cytokines [interleukin (IL)-6, IL-1ß, and IL-8], and enhanced the expression of antioxidant genes (quinine oxidoreductase 1, glutamate cysteine ligase catalytic subunit, heme oxygenase-1). It increased the expression of Keap1 and promoted the activation of Nrf2. However, the phosphorylation of IκBα and the nuclear expression of NF-κB p65 decreased. The anti-inflammatory and antioxidant effects of PO were abrogated following Nrf2 and NF-κB p65 knockdown. The results indicated a protective effect of PO against TNF-α-induced cell injury in A549 cells by modulating the balance between Nrf2 and NF-κB p65 signaling pathways. They verified PO as a promising anti-inflammatory adjuvant drug for treating ALI.


Assuntos
Apoptose/efeitos dos fármacos , Células Epiteliais/efeitos dos fármacos , Fator 2 Relacionado a NF-E2/metabolismo , NF-kappa B/antagonistas & inibidores , Óleos Voláteis/farmacologia , Fator de Necrose Tumoral alfa/farmacologia , Células A549 , Sobrevivência Celular/efeitos dos fármacos , Citocinas/metabolismo , Células Epiteliais/imunologia , Células Epiteliais/metabolismo , Humanos , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais
8.
Urol Oncol ; 36(7): 343.e9-343.e19, 2018 07.
Artigo em Inglês | MEDLINE | ID: mdl-29773494

RESUMO

BACKGROUND: PLIN3, one of the members of the perilipin family, has been reported to be involved in the formation and accumulation of lipid droplets. However, the expression levels and diagnostic and prognostic value of PLIN3 in renal cell carcinoma (RCC) remain unclear. METHODS: Bioinformatic analysis was used to assess the levels of PLIN3 and the correlation between PLIN3 levels and clinicopathological parameters in renal cancer. The expression levels of PLIN3 were determined in human RCC tissues and cell lines by western blot, immunofluorescence and immunohistochemistry assays. Receiver operating characteristic curves and Kaplan-Meier curves were used to analyze the diagnostic and prognostic significance of PLIN3 in RCC. RESULTS: The expression level of PLIN3 was elevated in RCC tissues and cell lines, which was consistent with the analysis of the TCGA and Oncomine cancer database. The receiver operating characteristic curve indicated that high PLIN3 expression can distinguish cancer tissues from normal kidney tissues (area under the curve = 0.7270, P<0.0001). Kaplan-Meier curves revealed that elevated PLIN3 predicted poor disease-free survival and overall survival. CONCLUSIONS: PLIN3 is highly expressed in kidney cancer, and high expression of PLIN3 can serve as a useful diagnostic and prognostic biomarker. PLIN3 functional inhibition can be used as a new clinical treatment option.


Assuntos
Biomarcadores Tumorais/metabolismo , Carcinoma de Células Renais/patologia , Neoplasias Renais/patologia , Recidiva Local de Neoplasia/patologia , Perilipina-3/metabolismo , Carcinoma de Células Renais/metabolismo , Carcinoma de Células Renais/cirurgia , Estudos de Casos e Controles , Bases de Dados Factuais , Feminino , Seguimentos , Humanos , Neoplasias Renais/metabolismo , Neoplasias Renais/cirurgia , Masculino , Pessoa de Meia-Idade , Recidiva Local de Neoplasia/metabolismo , Recidiva Local de Neoplasia/cirurgia , Prognóstico , Taxa de Sobrevida , Células Tumorais Cultivadas
9.
BMC Cancer ; 18(1): 88, 2018 01 22.
Artigo em Inglês | MEDLINE | ID: mdl-29357836

RESUMO

BACKGROUND: Scavenger receptor class B type I (SR-BI) has been reported to be involved in carcinogenesis of several human cancers. However, it is currently unknown whether SR-BI plays a role in clear cell renal cell carcinoma (ccRCC). Here, we aimed to evaluate a tumor promotive mechanism for SR-BI in ccRCC. METHODS: The expression of SR-BI was evaluated by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), Western blot and immunohistochemistry (IHC) in ccRCC tissues and cell lines. Lipid droplets in ccRCC tissues and normal kidney tissues were examined by Oil Red O (ORO) and hematoxylin-eosin (HE) staining. The correlation between SR-BI mRNA levels and clinicopathological features was analyzed by Pearson's chi-square test or Fisher's exact test. Kaplan-Meier analysis and Cox model were used to evaluate the difference in progression-free survival (PFS) associated with expression of SR-BI. Inhibition of SR-BI was conducted by using small interfering RNA (siRNA). In vitro assays were performed to assess the impact of SR-BI knockdown on cell biological behaviors. High density lipoprotein (HDL)-cholesterol content in ccRCC cells and extracellular media was also measured after transfection with siRNA. RESULTS: The expression of SR-BI was markedly up-regulated in ccRCC tissues and tumor cell lines. ORO and HE staining revealed huge amounts of lipid droplets accumulation in ccRCC. Clinical analysis showed that over-expression of SR-BI was positively associated with tumor size, grade, distant metastasis and inversely correlated with PFS. Furthermore, SR-BI was proved to be an independent prognostic marker in ccRCC patients. The inhibition of SR-BI attenuated the tumorous behaviors of ccRCC cells, expression of metastasis and AKT pathway related proteins. The content of HDL-cholesterol was reduced in cells while increased in extracellular media after transfection with si-SR-BI. CONCLUSIONS: Our results demonstrate that SR-BI functions as an oncogene and promotes progression of ccRCC. SR-BI may serve as a potential prognostic biomarker and therapeutic target for ccRCC.


Assuntos
Biomarcadores Tumorais/genética , Carcinoma de Células Renais/genética , Prognóstico , Receptores Depuradores Classe B/genética , Adulto , Idoso , Carcinoma de Células Renais/patologia , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , HDL-Colesterol/genética , Intervalo Livre de Doença , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Gotículas Lipídicas/metabolismo , Gotículas Lipídicas/patologia , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica/genética , Invasividade Neoplásica/patologia , RNA Mensageiro/genética
11.
Environ Sci Technol ; 51(22): 13502-13508, 2017 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-29076729

RESUMO

Lead (Pb) pollution emission from China is becoming a potential worldwide threat. Pb isotopic composition analysis is a useful tool to accurately trace the Pb sources of aerosols in atmosphere. In this study, a comprehensive data set of Pb isotopes for coals, Pb/Zn ores, and fuels from China was presented. The ratios of 206Pb/207Pb and 208Pb/206Pb in the coals were in the range of 1.114-1.383 and 1.791-2.317, similar to those from Europe, Oceania, and South Asia, but different from those from America (p < 0.01). The Pb/Zn ores had 206Pb/207Pb and 208Pb/206Pb in 1.020-1.183 and 2.088-2.309, less radiogenic than the coals. Leaded gasolines showed similar Pb isotopic compositions to Pb/Zn ores, with unleaded gasolines and diesels being mixed sources. The average Pb isotopic ratios of gasolines and diesels were significantly different (p < 0.01) from those of coals in China, leading to the possibility to discriminate Pb in fuels from in coals. Urban aerosols demonstrated similar Pb isotopic compositions to coals, Pb/Zn ores, and fuels in China. After removing the leaded gasoline, the Pb in aerosols is more radiogenic, supporting the heavy contribution of coal combustion to the atmospheric Pb pollution.


Assuntos
Carvão Mineral , Chumbo , Zinco , Ásia , China , Monitoramento Ambiental , Europa (Continente) , Isótopos
12.
Exp Cell Res ; 358(2): 269-278, 2017 09 15.
Artigo em Inglês | MEDLINE | ID: mdl-28684115

RESUMO

Caveolin-1 (CAV1) has been identified to be up-regulated in many cancers, including clear cell renal cell carcinoma (ccRCC). However, its potential function is still unclear in ccRCC. In this study, we demonstrated that CAV1 was frequently overexpressed in renal cell carcinoma tissues and cells, and was significantly associated with various clinicopathological parameters. In addition, high CAV1 expression was associated with poor disease-free survival (DFS) rate and could serve as a useful diagnostic indicator in ccRCC patients with different clinicopathological stages. Functional experiments demonstrated that CAV1 knockdown inhibited cell migration and invasion, whereas overexpression of CAV1 promoted cell migration and invasion in ccRCC. Moreover, CAV1 expression was up-regulated in sunitinib-resistant renal cancer cell lines, and its overexpression promoted sunitinib resistance. In general, our results confirm that CAV1 plays an important role in the metastasis of kidney cancer and induces sunitinib resistance, so CAV1 function suppression may become a promising clinical treatment strategy during renal cell carcinoma metastasis and sunitinib resistance.


Assuntos
Carcinoma de Células Renais/metabolismo , Caveolina 1/metabolismo , Movimento Celular/fisiologia , Resistencia a Medicamentos Antineoplásicos , Regulação Neoplásica da Expressão Gênica/genética , Indóis/farmacologia , Neoplasias Renais/metabolismo , Pirróis/farmacologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Caveolina 1/genética , Proliferação de Células/genética , Proliferação de Células/fisiologia , Feminino , Técnicas de Silenciamento de Genes/métodos , Humanos , Neoplasias Renais/patologia , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica , Prognóstico , Sunitinibe , Adulto Jovem
13.
Int J Mol Sci ; 18(3)2017 Feb 24.
Artigo em Inglês | MEDLINE | ID: mdl-28245556

RESUMO

Bleomycin (BLM), a family of anti-tumor drugs, was reported to exhibit severe side effects limiting its usage in clinical treatment. Therefore, finding adjuvants that enhance the anti-tumor effect and reduce the detrimental effect of BLM is a prerequisite. Chrysanthemum indicum, an edible flower, possesses abundant bioactivities; the supercritical-carbon dioxide fluid extract from flowers and buds of C. indicum (CISCFE) have strong anti-inflammatory, anti-oxidant, and lung protective effects. However, the role of CISCFE combined with BLM treatment on tumor-bearing mice remains unclear. The present study aimed to investigate the potential synergistic effect and the underlying mechanism of CISCFE combined with BLM in the treatment of hepatoma 22 (H22) tumor-bearing mice. The results suggested that the oral administration of CISCFE combined with BLM could markedly prolong the life span, attenuate the BLM-induced pulmonary fibrosis, suppress the production of pro-inflammatory cytokines (interleukin-6), tumor necrosis factor-α, activities of myeloperoxidase, and malondiadehyde. Moreover, CISCFE combined with BLM promoted the ascites cell apoptosis, the activities of caspases 3 and 8, and up-regulated the protein expression of p53 and down-regulated the transforming growth factor-ß1 by activating the gene expression of miR-29b. Taken together, these results indicated that CISCFE could enhance the anti-cancer activity of BLM and reduce the BLM-induced pulmonary injury in H22 tumor-bearing mice, rendering it as a potential adjuvant drug with chemotherapy after further investigation in the future.


Assuntos
Antibióticos Antineoplásicos/farmacologia , Bleomicina/farmacologia , Chrysanthemum/química , Extratos Vegetais/farmacologia , Animais , Antibióticos Antineoplásicos/efeitos adversos , Apoptose/efeitos dos fármacos , Bleomicina/efeitos adversos , Dióxido de Carbono , Caspase 3/metabolismo , Caspase 8/metabolismo , Linhagem Celular Tumoral , Citocinas/biossíntese , Modelos Animais de Doenças , Sinergismo Farmacológico , Fibrose , Extração Líquido-Líquido/métodos , Pulmão/metabolismo , Pulmão/patologia , Camundongos , MicroRNAs/genética , MicroRNAs/metabolismo , Neoplasias/tratamento farmacológico , Neoplasias/mortalidade , Neoplasias/patologia , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/química , Fator de Crescimento Transformador beta1/genética , Fator de Crescimento Transformador beta1/metabolismo , Carga Tumoral/efeitos dos fármacos , Proteína Supressora de Tumor p53/genética , Proteína Supressora de Tumor p53/metabolismo , Ensaios Antitumorais Modelo de Xenoenxerto
14.
J Med Food ; 20(2): 180-188, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28146409

RESUMO

Constipation is a common disorder that is a significant source of morbidity among people around the world ranging from 2% to 28%. Dendrobium officinale Kimura et Migo is a traditional herbal medicine and health food used for tonicity of the stomach and promotion of body fluid production in China. This study aimed to prepare the ultrafine powder of Dendrobium officinale (UDO) and investigate its laxative effect and potential mechanism in mice with diphenoxylate-induced constipation. Results indicated that the mean diameter (d50) of UDO obtained by ball milling was 6.56 µm. UDO (62.5, 125, and 250 mg/kg, p.o.) could significantly enhance the gastrointestinal transit ratio and promote fecal output. Moreover, UDO treatment resulted in significant increases in the serum levels of acetylcholinesterase (AChE), gastrin (Gas), motilin (MTL), and substance P (SP), and obviously decreased serum contents of somatostatin (SS). Taken together, UDO, which can be easily obtained through milling to a satisfactory particle size, exhibited obvious laxative effect in diphenoxylate-induced constipated mice, and the mechanism might be associated with elevated levels of AChE, Gas, MTL, SP, and reduced production of SS. UDO has the potential for further development into an alternative effective diet therapy for constipation.


Assuntos
Constipação Intestinal/tratamento farmacológico , Dendrobium/química , Laxantes/administração & dosagem , Extratos Vegetais/administração & dosagem , Animais , Constipação Intestinal/metabolismo , Constipação Intestinal/fisiopatologia , Feminino , Gastrinas/metabolismo , Trânsito Gastrointestinal/efeitos dos fármacos , Humanos , Laxantes/química , Masculino , Camundongos , Camundongos Endogâmicos ICR , Motilina/metabolismo , Extratos Vegetais/química , Substância P/metabolismo
15.
J Microbiol ; 55(4): 267-272, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28124776

RESUMO

This study details the introduction of a gfp marker into an endophytic bacterial strain (Achromobacter marplatensis strain 17, isolated from sugar beet) to monitor its colonization of sugar beet (Beta. vulgaris L.). Stability of the plasmid encoding the gfp was confirmed in vitro for at least 72 h of bacterial growth and after the colonization of tissues, under nonselective conditions. The colonization was observed using fluorescence microscopy and enumeration of culturable endophytes in inoculated sugar beet plants that grew for 10 or 20 days. gfp-Expressing strains were re-isolated from the inner tissues of surface-sterilized roots and stems of inoculated plants, and the survival of the Achromobacter marplatensis 17:gfp strain in plants 20 days after inoculation, even in the absence of selective pressure, suggests that it is good colonizer. These results also suggest that this strain could be a useful tool for the delivery of enzymes or other proteins into plants. In addition, the study highlights that sugar beet plants can be used effectively for detailed in vitro studies on the interactions between A. marplatensis strain 17 and its host, particularly if a gfp-tagged strain of the pathogen is used.


Assuntos
Achromobacter/crescimento & desenvolvimento , Beta vulgaris/microbiologia , Endófitos/crescimento & desenvolvimento , Achromobacter/química , Achromobacter/genética , Endófitos/química , Endófitos/genética , Instabilidade Genômica , Proteínas de Fluorescência Verde/análise , Proteínas de Fluorescência Verde/genética , Microscopia de Fluorescência , Raízes de Plantas/microbiologia , Caules de Planta/microbiologia , Plasmídeos , Proteínas Recombinantes/análise , Proteínas Recombinantes/genética , Coloração e Rotulagem
16.
Oncotarget ; 8(70): 114945-114955, 2017 Dec 29.
Artigo em Inglês | MEDLINE | ID: mdl-29383132

RESUMO

There is an urgent need for effective molecular therapies for hepatocellular carcinoma (HCC), the third-leading cause of cancer-related deaths worldwide. Norcantharidin (NCTD), a demethylated derivative of cantharidin, reportedly exhibits anticancer activity against various types of tumors, including HCC, though the mechanisms involved remain largely unknown. Here, we report that NCTD reduces viability of human MHCC-97H (97H) and HepG2 HCC cells, and induces cell death by triggering high levels of autophagy. Moreover, a significant attenuation of tumor growth was observed after NCTD treatment of HepG2 tumors in vivo, and this effect was enhanced by co-treatment with the c-Met inhibitor crizotinib. Interestingly, western blot analyses showed that the cytotoxic autophagy induced by NCTD correlates with a reduction in the phosphorylation status of both c-Met and m-TOR. These results suggest that cytotoxic autophagy resulting from inhibition of c-Met/mTOR signaling may be achieved in HCC by combined NCTD and crizotinib administration. Further studies to validate the therapeutic potential of this approach are warranted.

17.
Zhongguo Gu Shang ; 30(6): 557-560, 2017 Jun 25.
Artigo em Chinês | MEDLINE | ID: mdl-29424178

RESUMO

OBJECTIVE: To investigate surgical method and clinical curative effects of medial patellofemoral ligament (MPFL) reconstruction with great adductor muscular tendon in treating teenagers' recurrent patellar dislocation. METHODS: From May 2012 to September 2014, 19 patients with recurrent dislocation of patellar, including 6 males and 13 females with an average of 16 years old (ranged from 13 to 17 years), the courses of disease ranged from 3 to 18 months(averaged 6 months). All patients were underwent great adductor muscular tendon transposition to reconstruct medial patellofemoral ligament. The curative effects were evaluated by preoperative and postoperative with Lysholm scores and Patellofemoral angle and Q angle. RESULTS: All patients were followed up from 12 to 18 months with an average of 16.5 months. Primary healing was achieved at stage I. No pain, swelling and patellar dislocation or subluxation occurred. Patellofemoral angle increased from preoperative (-3.8±4.9)° to (10.3±4.1)° postoperatively. Q angle decreased from preoperative(16.4±3.1)° to(10.5±1.2)° postoperatively; Lysholm scores were improved from preoperative (68.6±8.5) to (93.7±6.5) final follow-up (P<0.01), and 15 cases got excellent results, 3 good, and 1 fair. CONCLUSIONS: Reconstruction of medial patellofemoral ligament with transposition of great adductor muscular tendon could obviously recover stability of patellar, and it is one of the effective methods for the treatment of teenagers' recurrent patellar dislocation.


Assuntos
Luxação Patelar/cirurgia , Ligamento Patelar/cirurgia , Tendões/transplante , Adolescente , Feminino , Humanos , Ligamentos Articulares/cirurgia , Masculino , Articulação Patelofemoral , Procedimentos Cirúrgicos Reconstrutivos/métodos , Recidiva , Resultado do Tratamento
18.
Urol Oncol ; 35(1): 36.e7-36.e14, 2017 01.
Artigo em Inglês | MEDLINE | ID: mdl-27633984

RESUMO

OBJECTIVES: Clear cell renal cell carcinoma (ccRCC) is the most frequent and lethal subtype of renal cell carcinoma, whose most effective measure of curing remains diagnosis and nephrectomy in its early phase. However, there is no feasible and recognized plasma biomarker for the clinical diagnosis of ccRCC. The objective of this study is to identify a novel plasma microRNA (miRNA) acting as an efficient diagnostic plasma biomarker in ccRCC. METHODS AND MATERIALS: The plasma miRNA expression profile was quantified by miRNA microarray. Validation of miRNA levels of plasmas and tissues were performed by quantitative reverse transcription polymerase chain reaction in 106 ccRCC, 28 renal angiomyolipomas (AML), and 123 healthy control plasmas and in 110 ccRCC tissues. RESULTS: We found that plasma miR-144-3p levels in 106 ccRCC plasmas were remarkably up-regulated compared with that in healthy individuals and in patients with AML. miR-144-3p served as a promising plasma biomarker for yielding an area under the receiver operating characteristic curve of 0.91 with 87.10% sensitivity and 83.02% specificity in discriminating ccRCC from healthy individuals, and an area under the curve of 0.82 with 75.00% sensitivity and 71.70% specificity in discriminating ccRCC from patients with AML. In addition, plasma miR-144-3p levels were significantly decreased after surgery in 106 patients with ccRCC. Next, we examined miR-144-3p levels in 110 human ccRCC tissues, and found that miR-144-3p levels in ccRCC tissues were increased compared with adjacent normal tissues. Pearson correlation analysis revealed that miR-144-3p levels in tumor tissues were positively correlated with preoperative plasma miR-144-3p levels in the matched samples from patients with ccRCC. In addition, the miR-144-3p levels in ccRCC plasmas and tissues were increased in patients with advanced pT stage. CONCLUSIONS: Our data indicate that miR-144-3p, which is significantly up-regulated in ccRCC plasmas and tissues, particularly with advanced pT stage, is a novel and excellent plasma biomarker for the diagnosis of ccRCC.


Assuntos
Angiomiolipoma/diagnóstico , Carcinoma de Células Renais/diagnóstico , Carcinoma de Células Renais/metabolismo , Neoplasias Renais/diagnóstico , Neoplasias Renais/metabolismo , MicroRNAs/sangue , Angiomiolipoma/sangue , Área Sob a Curva , Biomarcadores Tumorais/sangue , Carcinoma de Células Renais/patologia , Carcinoma de Células Renais/cirurgia , Feminino , Humanos , Rim/metabolismo , Neoplasias Renais/patologia , Neoplasias Renais/cirurgia , Masculino , MicroRNAs/metabolismo , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Nefrectomia , Análise de Sequência com Séries de Oligonucleotídeos , Curva ROC , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Regulação para Cima
19.
Clin Chim Acta ; 464: 218-222, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-27919602

RESUMO

BACKGROUND: We evaluated whether alterations of hemoglobin (HB), hematocrit (HCT), serum albumin level (ALB), and the difference of HCT and ALB can be used in the diagnosis of preeclampsia and eclampsia in patients with hypertensive disorders of pregnancy (HDP). METHODS: A total of 509 individuals were recruited and divided into 4 groups: Group 1, 170 healthy non-pregnant women; Group 2, 125 normal pregnant women; Group 3, 105 pregnant women diagnosed with gestational and chronic hypertension; Group 4, 109 pregnant women diagnosed as having preeclampsia and eclampsia. Data of HB, HCT, ALB, globulin (GLB) were collected at the time of a prenatal examination during the third trimester. RESULTS: Alterations in the HCT and the ALB levels in these groups were significantly different. Group 4 had a higher mean HCT-ALB value (P<0.01), but lower ALB and GLB values compared with the other three groups. We used Groups 2 and 3 as the respective reference to draw the receiver operating characteristic (ROC) curves of HCT-ALB in Group 4, and found that the threshold values of maximum index corresponding were 12.95 and 12.65 (sensitivity>57.0%, specificity>98.9%), respectively. CONCLUSIONS: The value of HCT-ALB>12.65 might be used as a potential biomarker for the auxiliary diagnosis of preeclampsia and eclampsia in HDP.


Assuntos
Eclampsia/sangue , Eclampsia/diagnóstico , Hematócrito , Pré-Eclâmpsia/sangue , Pré-Eclâmpsia/diagnóstico , Albumina Sérica/metabolismo , Adulto , Biomarcadores/sangue , Diagnóstico Diferencial , Feminino , Humanos , Gravidez , Curva ROC , Adulto Jovem
20.
Int J Syst Evol Microbiol ; 66(12): 5523-5528, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27902191

RESUMO

A Gram-stain-negative, short rod-shaped and light-red-pigmented bacterium, designated XAAS-1T, was isolated from the soil of Aydingkol Lake near the Turpan City, Xinjiang, China. The isolate was positive for oxidase, catalase and hydrolysis of starch, casein, gelatin and aesculin. The sole respiratory quinone was MK-7 and the principal cellular fatty acids were iso-C15 : 0 and C15 : 0 3-OH. The major polar lipids were phosphatidylethanolamine, one unidentified phospholipid and two unidentified polar lipids. The polyamine pattern was found to contain mainly sym-homospermidine. 16S rRNA gene sequence analysis indicated that strain XAAS-1T belongs to the genus Pontibacter in the family Cytophagaceae, with sequence similarities ranging from 93.8 to 96.7 % with other type species of the genus Pontibacter. On the basis of phenotypic and genotypic data, strain XAAS-1T represents a novel species of the genus Pontibacter, for which the name Pontibacter aydingkolensis sp. nov. (type strain XAAS-1T=CCTCC AB 2016134T=JCM 31442T) is proposed.


Assuntos
Cytophagaceae/classificação , Lagos/química , Filogenia , Salinidade , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Composição de Bases , China , Cytophagaceae/genética , Cytophagaceae/isolamento & purificação , DNA Bacteriano/genética , Ácidos Graxos/química , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Cloreto de Sódio , Solo , Espermidina/análogos & derivados , Espermidina/química , Vitamina K 2/análogos & derivados , Vitamina K 2/química
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