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1.
Nano Lett ; 2020 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-32391698

RESUMO

Catalytic water splitting driven by renewable electricity offers a promising strategy to produce molecular hydrogen, but its efficiency is severely restricted by the sluggish kinetics of the anodic water oxidation reaction. Amorphous catalysts are reported to show better activities of water oxidation than their crystalline counterparts, but little is known about the underlying origin, which retards the development of high-performance amorphous oxygen evolution reaction catalysts. Herein, on the basis of cyclic voltammetry, electrochemical impedance spectroscopy, isotope labeling, and in situ X-ray absorption spectroscopy studies, we demonstrate that an amorphous catalyst can be electrochemically activated to expose active sites in the bulk thanks to the short-range order of the amorphous structure, which greatly increases the number of active sites and thus improves the electrocatalytic activity of the amorphous catalyst in water oxidation.

2.
Soft Matter ; 16(16): 4024-4031, 2020 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-32270152

RESUMO

The gemini-like surfactants have been constructed by compounding N-erucamidopropyl-N,N-dimethylamine (UC22AMPM) and o-phthalic acid (o-PA), m-phthalic acid (m-PA), or p-phthalic acid (p-PA), and are denoted as o-EAPA, m-EAPA, and p-EAPA, respectively. It is well known that inorganic salts have significant effects on surfactant aggregates, and herein the effects of sodium chloride (NaCl) on gemini-like surfactants is explored by rheological and dynamic light scattering measurements, and cryo-TEM. It is found that the viscoelasticity of the EAPA systems first increases and then decreases with an increase of the NaCl concentration. And the optimal NaCl concentrations for these three systems are in the order of o-EAPA < m-EAPA < p-EAPA due to different spacer distances between the two carboxyl groups in the phthalic acid. Similar trends in the N,N-dimethyl oleoaminde-propylamine (DOAPA) and o-PA, m-PA, or p-PA systems were also observed. The results show that an appropriate NaCl concentration will promote gemini-like surfactants to form wormlike micelles (WLMs). Upon further increasing the NaCl concentration, the WLMs transform into vesicles. Excessive NaCl concentration will cause the surfactant systems to reach their cloud point and make the surfactants precipitate out. The mechanism of the effects of NaCl is that Cl- reduces the electrostatic repulsion between the headgroups of the surfactants. This work is helpful in understanding the effects of inorganic salts on the surfactants and this study is useful for exploring the practical applications of gemini-like surfactants.

3.
Artigo em Inglês | MEDLINE | ID: mdl-32329173

RESUMO

Single-atom catalysts (SACs) show great promise for electrochemical CO2 reduction reaction (CRR), but the low density of active sites and the poor electrical conduction and mass transport of the single-atom electrode greatly limit their performance. Herein, we prepared a nickel single-atom electrode consisting of isolated, high-density and low-valent nickel(I) sites anchored on a self-standing N-doped carbon nanotube array with nickel-copper alloy encapsulation on a carbon-fiber paper. The combination of single-atom nickel(I) sites and self-standing array structure gives rise to an excellent electrocatalytic CO2 reduction performance. The introduction of copper tunes the d-band electron configuration and enhances the adsorption of hydrogen, which impedes the hydrogen evolution reaction. The single-nickel-atom electrode exhibits a specific current density of -32.87 mA cm-2 and turnover frequency of 1962 h-1 at a mild overpotential of 620 mV for CO formation with 97 % Faradic efficiency.

4.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi ; 34(2): 184-189, 2020 Feb 15.
Artigo em Chinês | MEDLINE | ID: mdl-32030949

RESUMO

Objective: To summarize the effectiveness of two-stage reverse total shoulder arthroplasty for treating postoperative deep infection after internal fixation of the proximal humeral fracture. Methods: Between June 2014 and January 2018, 17 patients with deep infection and humeral head necrosis or bone nonunion after internal fixation of proximal humeral fractures were treated. There were 8 males and 9 females, aged from 52 to 78 years (mean, 63.8 years). The infection occurred at 19-66 months after the initial internal fixation (mean, 34.8 months). Microbial culture of joint fluid was positive in 14 cases and negative in 3 cases. The preoperative Constant score, American shoulder and elbow surgeons (ASES) score, and visual analogue scale (VAS) score were 36.41±8.65, 31.06±7.43, and 7.29±0.99, respectively. The preoperative ranges of forward flexion, abduction, external rotation were (45.88±12.46), (42.18±12.31), and (16.76±4.92)°, respectively. The preoperative range of internal rotation was buttock in 9 cases, lumbosacral joint in 3 cases, L 3 in 5 cases. At the first-stage surgery, the thorough debridement was done and the antibiotic-impregnated bone cement spacer was placed after the removal of internal fixation. After the infections disappeared, the two-stage reverse total shoulder arthroplasty was performed. The mean interval between the two procedures was 4.2 months (range, 3.0-6.5 months). Results: All the incisions healed primarily and no complications such as recurrent infection or vascular nerve injury occurred. All patients were followed up 15-32 months (mean, 22.0 months). At last follow-up, the ranges of forward flexion, abduction, and external rotation were (109.00±23.66), (98.53±16.92), (41.41±6.82)°, respectively; and the range of internal rotation was lumbosacral joint in 5 cases, L 3 in 8 cases, T 12 in 4 cases. The range of motion of shoulder joints at last follow-up was significant improved when compared with the preoperative range of motion ( P<0.05). The Constant score (64.88±8.70), ASES score (65.18±8.10), and VAS score (2.94±1.25) were significantly superior to the preoperative scores ( P<0.05). X-ray films showed that no prosthesis loosening occurred. Conclusion: Two-stage reverse total shoulder arthroplasty is an effective treatment for the postoperative deep infection after internal fixation of the proximal humeral fracture, which has advantages of low risk of infection recurrence, good shoulder function, and satisfactory short-term effectiveness.


Assuntos
Artroplastia do Ombro , Complicações Pós-Operatórias , Fraturas do Ombro , Articulação do Ombro , Idoso , Artroplastia do Ombro/efeitos adversos , Feminino , Fixação Interna de Fraturas , Humanos , Masculino , Pessoa de Meia-Idade , Amplitude de Movimento Articular , Estudos Retrospectivos , Fraturas do Ombro/cirurgia , Resultado do Tratamento
5.
Chem Res Toxicol ; 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-32091207

RESUMO

Structural alerts are a simple and easy way to identify toxic compounds being widely used in environmental toxicology research and drug discovery. With the emergence of big data techniques in recent years and their applications in chemistry and toxicology, computational approaches have become a promising method to identify structural alerts. In this Review, we describe the recent progress in computational methods for identification of structural alerts and their applications in toxicology. Two major computational approaches, namely frequency analysis and interpretable machine learning models, are reviewed. Recent studies have shown that both approaches are superior to expert systems with respect to predictive capability. Methodologies for defining the applicability domain of such approaches are also reviewed, with their importance stemming from their ability to not only improve the predictive performance of structural alert models but also ensure the confidence of a prediction. In addition to toxicity prediction, structural alerts could be also used to explain quantitative structure-activity relationship models and guide lead optimization in drug discovery. Nevertheless, there are still some challenges to be solved, such as how to address the co-existence of several structural alerts in one molecule, how to directly compare computationally derived structural alerts with expert systems, and how to explore new mechanisms of toxicity.

6.
Chem Res Toxicol ; 33(2): 640-650, 2020 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-31957435

RESUMO

Renal clearance (CLr) plays an essential role in the elimination of drugs. In this study, 636 compounds were obtained from various sources to develop in silico models for the prediction of CLr. Stepwise multiple linear regression and random forest regression methods were employed to build global models and local models according to ionization state or net elimination pathways. The local models toward compounds undergoing different net elimination pathways showed good predictive power: the geometric mean fold error was close to 2, indicating the clearance of most compounds could be predicted within a 2-fold error range. Six classification methods were used to construct classification models. However, the performance of these classification models was less than satisfactory, and the mean accuracy of the top five models in test sets was 0.65. Moreover, qualitative analysis of physicochemical profiles between compounds undergoing different net elimination pathways revealed that compounds with higher lipophilicity tended to be reabsorbed more easily and showed lower CLr, while compounds with higher values of polar descriptors tended to secrete more easily and showed higher CLr.

7.
J Gastroenterol ; 55(2): 159-168, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31679066

RESUMO

BACKGROUND: Peroral endoscopic myotomy (POEM) has shown excellent results for the treatment of achalasia in adults, but studies for children are limited. The study was aimed to analyze outcomes of peroral endoscopic myotomy (POEM) in children and compared with those in adults in a large multi-center study. METHODS: Records of consecutive patients with achalasia who underwent POEM at three tertiary centers were reviewed. A total of 130 children were included in this study. The primary outcomes of perioperative outcomes and clinical follow-up data were analyzed. RESULTS: One child (0.8%) experienced technical failure. Five children (3.8%) had major adverse events, including one with pneumothorax requiring drainage, two with delayed mucosa barrier failure, one with readmission, and one with vital-sign instability. Both post-POEM Eckardt score and median LES pressure were significantly lower than their pre-POEM reference values in children (0.7 vs 7.4; 7.0 vs 27.1 mmHg; both P < 0.001). During a median follow-up time of 40 months, clinical reflux rate was 27.0% and clinical failure rates at 1, 3, and 5 years were 1.8%, 3.5%, and 4.4% for children. The technical failure, major adverse events, and postoperative clinical reflux were comparable between children and adults (all P > 0.05). Kaplan-Meier analysis showed that the risk of clinical failure was lower in children than adults (log-rank test, hazard ratio = 0.37, 95% confidence interval 0.15-0.91, P = 0.023). CONCLUSIONS: POEM can be safely performed in children with achalasia, and produce a better clinical response during long-term follow-up compared with that in adults.

8.
Chem Commun (Camb) ; 55(100): 15065-15068, 2019 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-31777871

RESUMO

Graphitic carbon nitride (g-CN) nanosheets (CNNs) with an ultra-high quantum yield (80.1%) ultraviolet fluorescence (FL) were prepared. The effects of the lateral size and the polymerization temperature on the optical properties of CNNs have been studied. The ultraviolet FL was proved to have originated from the isolated melem units according to the density functional theory calculation and mass spectra. The obtained CNNs are further used as a pH probe due to the dependence of the FL signal on the pH of the solution.

9.
Neuron ; 104(5): 899-915.e8, 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31672263

RESUMO

Chronic stress (CS) is a major risk factor for the development of depression. Here, we demonstrate that CS-induced hyperactivity in ventral tegmental area (VTA)-projecting lateral habenula (LHb) neurons is associated with increased passive coping (PC), but not anxiety or anhedonia. LHb→VTA neurons in mice with increased PC show increased burst and tonic firing as well as synaptic adaptations in excitatory inputs from the entopeduncular nucleus (EP). In vivo manipulations of EP→LHb or LHb→VTA neurons selectively alter PC and effort-related motivation. Conversely, dorsal raphe (DR)-projecting LHb neurons do not show CS-induced hyperactivity and are targeted indirectly by the EP. Using single-cell transcriptomics, we reveal a set of genes that can collectively serve as biomarkers to identify mice with increased PC and differentiate LHb→VTA from LHb→DR neurons. Together, we provide a set of biological markers at the level of genes, synapses, cells, and circuits that define a distinctive CS-induced behavioral phenotype.

10.
Theranostics ; 9(26): 8409-8425, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31754405

RESUMO

Introduction: Metastasis and drug resistance contribute substantially to the poor prognosis of colorectal cancer (CRC) patients. However, the epigenetic regulatory mechanisms by which CRC develops metastatic and drug-resistant characteristics remain unclear. This study aimed to investigate the role of miR-302a in the metastasis and molecular-targeted drug resistance of CRC and elucidate the underlying molecular mechanisms. Methods: miR-302a expression in CRC cell lines and patient tissue microarrays was analyzed by qPCR and fluorescence in situ hybridization. The roles of miR-302a in metastasis and cetuximab (CTX) resistance were evaluated both in vitro and in vivo. Bioinformatic prediction algorithms and luciferase reporter assays were performed to identify the miR-302a binding regions in the NFIB and CD44 3'-UTRs. A chromatin immunoprecipitation assay was performed to examine NFIB occupancy in the ITGA6 promoter region. Immunoblotting was performed to identify the EGFR-mediated pathways altered by miR-302a. Results: miR-302a expression was frequently reduced in CRC cells and tissues, especially in CTX-resistant cells and patient-derived xenografts. The decreased miR-302a levels correlated with poor overall CRC patient survival. miR-302a overexpression inhibited metastasis and restored CTX responsiveness in CRC cells, whereas miR-302a silencing exerted the opposite effects. NFIB and CD44 were identified as novel targets of miR-302a. miR-302a inhibited the metastasis-promoting effect of NFIB that physiologically activates ITGA6 transcription. miR-302a restored CTX responsiveness by suppressing CD44-induced cancer stem cell-like properties and EGFR-mediated MAPK and AKT signaling. These results are consistent with clinical observations indicating that miR-302a expression is inversely correlated with the expression of its targets in CRC specimens. Conclusions: Our findings show that miR-302a acts as a multifaceted regulator of CRC metastasis and CTX resistance by targeting NFIB and CD44, respectively. Our study implicates miR-302a as a candidate prognostic predictor and a therapeutic agent in CRC.

11.
Nat Commun ; 10(1): 4633, 2019 10 11.
Artigo em Inglês | MEDLINE | ID: mdl-31604921

RESUMO

The dorsal raphe (DR) is a heterogeneous nucleus containing dopamine (DA), serotonin (5HT), γ-aminobutyric acid (GABA) and glutamate neurons. Consequently, investigations of DR circuitry require Cre-driver lines that restrict transgene expression to precisely defined cell populations. Here, we present a systematic evaluation of mouse lines targeting neuromodulatory cells in the DR. We find substantial differences in specificity between lines targeting DA neurons, and in penetrance between lines targeting 5HT neurons. Using these tools to map DR circuits, we show that populations of neurochemically distinct DR neurons are arranged in a stereotyped topographical pattern, send divergent projections to amygdala subnuclei, and differ in their presynaptic inputs. Importantly, targeting DR DA neurons using different mouse lines yielded both structural and functional differences in the neural circuits accessed. These results provide a refined model of DR organization and support a comparative, case-by-case evaluation of the suitability of transgenic tools for any experimental application.


Assuntos
Núcleo Dorsal da Rafe/fisiologia , Modelos Animais , Neurotransmissores/genética , Animais , Neurônios Dopaminérgicos/metabolismo , Camundongos , Camundongos Transgênicos , Vias Neurais , Neurônios Serotoninérgicos/metabolismo , Proteínas da Membrana Plasmática de Transporte de Serotonina/genética , Proteínas da Membrana Plasmática de Transporte de Serotonina/metabolismo
12.
Toxicol Lett ; 317: 68-81, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31580885

RESUMO

Skin sensitization, frequently leading to allergic contact dermatitis (ACD), is authenticated to be a significant endpoint in the field of drug discovery and cosmetics. The initiation of ACD, also known as the skin sensitization mechanism, has been documented as an adverse outcome pathway (AOP), which can be studied experimentally and computationally. In this study, we collected 154 haptens and applied systems toxicology methods to develop a reaction-substructure-compound- target-pathway network system. For the collected haptens, their key substructures were identified and associated with their protein binding reactions. The targets of haptens, including the known targets collected from four databases and the potential targets predicted via our balanced substructure-drug-target network-based inference (bSDTNBI) method, were matched to skin proteins to obtain skin targets. The dermatitis-related pathways were enriched and were subject to literature verification. The network system we developed can be applied to predict the reactions, targets and pathways of new haptens, which contributed to evaluating chemical safety and optimizing chemical structures. The study of skin sensitization mechanism is helpful for understanding the skin immunity and resisting ACD.


Assuntos
Dermatite Alérgica de Contato/etiologia , Haptenos/toxicidade , Pele/efeitos dos fármacos , Biologia de Sistemas , Toxicologia/métodos , Animais , Dermatite Alérgica de Contato/imunologia , Dermatite Alérgica de Contato/metabolismo , Haptenos/química , Humanos , Estrutura Molecular , Ligação Proteica , Mapas de Interação de Proteínas , Medição de Risco , Transdução de Sinais/efeitos dos fármacos , Pele/imunologia , Pele/metabolismo , Relação Estrutura-Atividade
13.
EMBO J ; 38(20): e101443, 2019 10 15.
Artigo em Inglês | MEDLINE | ID: mdl-31424118

RESUMO

Cyclins are central engines of cell cycle progression in conjunction with cyclin-dependent kinases (CDKs). Among the different cyclins controlling cell cycle progression, cyclin F does not partner with a CDK, but instead forms via its F-box domain an SCF (Skp1-Cul1-F-box)-type E3 ubiquitin ligase module. Although various substrates of cyclin F have been identified, the vulnerabilities of cells lacking cyclin F are not known. Thus, we assessed viability of cells lacking cyclin F upon challenging them with more than 180 different kinase inhibitors. The screen revealed a striking synthetic lethality between Chk1 inhibition and cyclin F loss. Chk1 inhibition in cells lacking cyclin F leads to DNA replication catastrophe. Replication catastrophe depends on accumulation of the transcription factor E2F1 in cyclin F-depleted cells. We find that SCF-cyclin F controls E2F1 ubiquitylation and degradation during the G2/M phase of the cell cycle and upon challenging cells with Chk1 inhibitors. Thus, Cyclin F restricts E2F1 activity during the cell cycle and upon checkpoint inhibition to prevent DNA replication stress. Our findings pave the way for patient selection in the clinical use of checkpoint inhibitors.


Assuntos
Quinase 1 do Ponto de Checagem/antagonistas & inibidores , Ciclinas/metabolismo , Fator de Transcrição E2F1/metabolismo , Inibidores de Proteínas Quinases/farmacologia , Proteólise , Proteínas Ligases SKP Culina F-Box/metabolismo , Mutações Sintéticas Letais , Ciclo Celular/efeitos dos fármacos , Quinase 1 do Ponto de Checagem/genética , Ciclinas/genética , Replicação do DNA , Fator de Transcrição E2F1/genética , Células HeLa , Humanos , Fosforilação , Ligação Proteica , Proteínas Ligases SKP Culina F-Box/genética , Ubiquitinação
14.
Cell Rep ; 28(3): 616-624.e5, 2019 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-31315042

RESUMO

Overeating is a serious issue in modern society, causing many health problems, including obesity. Although the hypothalamus has been previously identified as the key brain structure that regulates body weight homeostasis, the downstream pathways and non-canonical neural circuitry involved in feeding behavior remain largely uncharacterized. Here, we discover that suppressing the activity of GABAergic cells in the anterior ventrolateral periaqueductal gray (vlPAG), whether directly or through long-projection GABAergic inputs from either the bed nucleus of the stria terminalis (BNST) or the lateral hypothalamus (LH), is sufficient to promptly induce feeding behavior in well-fed mice. In contrast, optogenetic activation of these cells interrupts food intake in starved mice. Long-term chemogenetic manipulation of vlPAG GABAergic cell activity elicits a corresponding change in mouse body weight. Our studies reveal distinct midbrain GABAergic pathways and highlight an important role of GABAergic cells in the anterior vlPAG in feeding behavior.

15.
Toxicol Res (Camb) ; 8(3): 341-352, 2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31160968

RESUMO

Aquatic toxicity is a crucial endpoint for evaluating chemically adverse effects on ecosystems. Therefore, we developed in silico methods for the prediction of chemical aquatic toxicity in marine environment. At first, a diverse data set including different crustacean species was constructed. We then built local binary models using Mysidae data and global binary models using Mysidae, Palaemonidae, and Penaeidae data. Molecular fingerprints and descriptors were employed to represent chemical structures separately. All the models were built by six machine learning methods. The AUC (area under the receiver operating characteristic curve) values of the better local and global models were around 0.8 and 0.9 for the test sets, respectively. We also identified several chemicals with selective toxicity on different species. The analysis of selective toxicity would promote to design greener chemicals in a specific environment. Finally, to understand and interpret the models, we explored the relationships between chemical aquatic toxicity and the molecular descriptors. Our study would be helpful in gaining further insights into marine organisms, prediction of chemical aquatic toxicity and prioritization of environmental hazard assessment.

16.
Neurosci Bull ; 35(5): 781-790, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31168753

RESUMO

The laterodorsal tegmentum (LDT) is a brain structure involved in distinct behaviors including arousal, reward, and innate fear. How environmental stimuli and top-down control from high-order sensory and limbic cortical areas converge and coordinate in this region to modulate diverse behavioral outputs remains unclear. Using a modified rabies virus, we applied monosynaptic retrograde tracing to the whole brain to examine the LDT cell type specific upstream nuclei. The LDT received very strong midbrain and hindbrain afferents and moderate cortical and hypothalamic innervation but weak connections to the thalamus. The main projection neurons from cortical areas were restricted to the limbic lobe, including the ventral orbital cortex (VO), prelimbic, and cingulate cortices. Although different cell populations received qualitatively similar inputs, primarily via afferents from the periaqueductal gray area, superior colliculus, and the LDT itself, parvalbumin-positive (PV+) GABAergic cells received preferential projections from local LDT neurons. With regard to the different subtypes of GABAergic cells, a considerable number of nuclei, including those of the ventral tegmental area, central amygdaloid nucleus, and VO, made significantly greater inputs to somatostatin-positive cells than to PV+ cells. Diverse inputs to the LDT on a system-wide level were revealed.

17.
Toxicol Lett ; 312: 22-33, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31063831

RESUMO

Liver is the central place for drug metabolism. Drug-induced liver injury (DILI) is hence inevitable, and has become one of the leading causes for drug failure in development and drug withdrawal from the market. Due to lack of reliable preclinical and in vivo toxicology test conditions, it is time-consuming, laborious and costly to interpret the mechanisms of DILI through bioassays. In this paper, we developed a computational systems toxicology approach to investigate the molecular mechanisms of DILI. Totally 1478 DILI compounds were collected, together with 1067 known targets for 896 DILI compounds. Then, 173 new potential targets of these compounds were predicted by our bSDTNBI (balanced substructure-drug-target network-based inference) method. After network analysis, 145 primary genes were found to relate with hepatotoxicity and have higher expression in liver, among which 26 genes were predicted by our method, such as CYP2E1, GSTA1, EPHX1, ADH1B, ADH1C, ALDH2, F7, and IL2. A scoring function, DILI-Score, was further proposed to assess the hepatotoxic severity of a given compound. Finally, as case studies, we analyzed the mechanisms of DILI from the perspective of off-targets, and found out the pivotal genes for liver injuries induced by tyrosine kinase inhibitors and TAK-875. This work would be helpful for better understanding mechanisms of DILI and provide clues for reducing risk of DILI.


Assuntos
Doença Hepática Induzida por Substâncias e Drogas/patologia , Simulação por Computador , Efeitos Colaterais e Reações Adversas Relacionados a Medicamentos , Modelos Biológicos , Animais , Biologia Computacional , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Fígado/metabolismo
18.
Food Chem ; 292: 314-324, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31054680

RESUMO

Little is known about the variations of fresh fruit biomembrane and its physiological and biochemical characteristics during storage. A navel orange mutant 'Gannan No.1' (Citrus sinensis Osbeck) showed higher membrane stability and titratable acid while lower calyx senescence compared with wild-type 'Newhall'. The membrane damage was significantly reduced in 'Gannan No.1' under 10% polyethylene-glycol (41.16% vs. 8.77%) and 30% polyethylene-glycol (52.59% vs.16.11%) treatments on day 45 after harvest. Consistently, membrane electrolyte leakage and malondialdehyde were significantly decreased in 'Gannan No.1', and superoxide dismutase and glutathione reductase were activated. A metabolic analysis was performed to evaluate membrane fatty acid unsaturation and peroxidation. Linolenic acid and hexadecylenic acid contributed to the higher degree of unsaturated fatty acids in 'Gannan No.1'. Furthermore, 'Gannan No.1' accumulated stress-resistant metabolites such as proline, α-tocopherol and glutathione. Correlation analysis of membrane homeostasis indexes with quality parameters showed the importance of biomembrane stability in maintaining citrus fruit quality.


Assuntos
Parede Celular/metabolismo , Citrus sinensis/metabolismo , Ácidos Graxos/metabolismo , Peroxidação de Lipídeos , Parede Celular/química , Citrus sinensis/química , Citrus sinensis/genética , Armazenamento de Alimentos , Frutas/química , Frutas/metabolismo , Glutationa Redutase/metabolismo , Malondialdeído/análise , Malondialdeído/metabolismo , Potenciais da Membrana , Mutação , Fenótipo , Proteínas de Plantas/metabolismo , Polietilenoglicóis/química , Análise de Componente Principal , Superóxido Dismutase/metabolismo
19.
J Craniomaxillofac Surg ; 47(7): 1110-1119, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30962040

RESUMO

PURPOSE: To compare the functional outcomes between open reduction and internal fixation (ORIF) and closed reduction (CR) for unilateral mandibular extra-capsular condylar fractures in patients over 12 years old. MATERIALS AND METHODS: A comprehensive electronic search of PubMed, Embase and the Cochrane Library databases was conducted up to October 31, 2018. The evaluated functional outcomes included malocclusion, temporomandibular joint (TMJ) pain, protrusion, laterotrusion, maximum inter-incisal opening and lateral deviation during maximum inter-incisal opening. RESULTS: Fourteen studies appeared to meet the inclusion criteria. Statistically significant differences between ORIF and CR treatment were observed for the outcomes of malocclusion (P = 0.001), maximum inter-incisal opening (P = 0.0008), lateral deviation during maximum inter-incisal opening (P = 0.007) and laterotrusion (P < 0.0001), but not for the outcomes of protrusion (P = 0.33) and TMJ pain (P = 0.29). CONCLUSIONS: ORIF treatment of unilateral mandibular extra-capsular condylar fractures provides better functional outcomes in comparison to CR treatment with regard to occlusion, maximum inter-incisal opening, lateral deviation during maximum inter-incisal opening and laterotrusion, whereas there was no statistically significant difference between ORIF and CR group with regard to protrusion and TMJ pain.


Assuntos
Fraturas Mandibulares , Fixação Interna de Fraturas , Humanos , Técnicas de Fixação da Arcada Osseodentária , Côndilo Mandibular , Fraturas Mandibulares/cirurgia , Redução Aberta , Amplitude de Movimento Articular , Resultado do Tratamento
20.
Medicine (Baltimore) ; 98(12): e14820, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30896625

RESUMO

RATIONALE: Gastric duplication cyst is an anomaly that primarily occurs to children. Apart from the conventional use of surgical resection, few cases using endoscopic treatment have been reported. PATIENT CONCERNS: A 5-month-old female infant was hospitalized with the chief complaint of gastric cyst. No significant abnormalities were identified by physical examination. INTERVENTIONS: Endoscopic submucosal dissection (ESD) was performed successfully for the infant and the duration was less than 20 minutes. The patient showed no postoperative complications. OUTCOMES: At 4 months during the follow-up, upper endoscopy revealed a small scar at the previous site of the lesion and no recurrence. LESSONS: According to the results of PUBMED review, she was the youngest with gastric duplication cyst removed with ESD. The less invasive ESD should be considered an effective therapeutic option to remove gastric duplication cyst in children.


Assuntos
Cistos/cirurgia , Ressecção Endoscópica de Mucosa/métodos , Gastropatias/cirurgia , Feminino , Mucosa Gástrica , Humanos , Lactente , Duração da Cirurgia
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