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1.
Exp Ther Med ; 23(1): 93, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34976135

RESUMO

Combined esophageal atresia (EA), tracheoesophageal fistula (TEF) and duodenal obstruction result in various challenges in management, and a well-defined management protocol is still lacking. Esophageal stricture is the most common complication after EA repair. The use of magnetic compression alimentary tract anastomosis has been reported in children. By searching the literature, the present study reports the first case of simultaneous repair (EA repair followed by duodenal obstruction repair) and magnetic compression stricturoplasty for refractory esophageal stricture after EA repair in two male neonates. One of the neonates received delayed treatment of duodenal obstruction, and the other successfully underwent a simultaneous emergency operation of these combined anomalies. These two infants developed refractory strictures despite multiple endoscopic dilatation procedures during the postoperative follow-up period. Magnetic compression stricturoplasty procedures were successfully performed under fluoroscopic and endoscopic guidance without any leakage or complication. At the follow-up 10-months after stricturoplasty, the two patients achieved durable esophageal patency in the absence of dysphagia. Combination of early chest and abdominal X-ray detection is recommended to avoid a delayed diagnosis and treatment, as well as the synchronous operation for EA/TEF repair and duodenoduodenostomy in a single surgery for combined EA/TEF and duodenal obstructions. Therefore, magnetic compression stricturoplasty is a feasible and efficient method for establishing early patency of the esophagus in patients with refractory EA stricture.

2.
Food Chem ; 375: 131833, 2022 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-34974349

RESUMO

Hypoxia frequently occurs in postharvest logistics, which greatly influences fruit storability. Here, we for the first time studied the dynamic variations of mitochondrial morphology in living citrus fruit cells, and revealed that waxing treatment-induced hypoxia strongly triggered mitochondrial fission and fragmentation. Correspondingly, hypoxia caused a decline in mitochondrial membrane potential and mobility. Besides, impairment of energetic and redox status was also found in waxed fruit. The proteomic changes of mitochondria after waxing treatment were also characterized. Using weighted gene co-expression network analysis (WGCNA), we identified 167 key hypoxia-responsive proteins, which were mainly involved in fatty acid, amino acid and organic acid metabolism. Metabolite analysis verified that waxing treatment promoted the accumulation of several hypoxic metabolites, such as ethanol, acetaldehyde, succinic acid and γ-aminobutyric acid (GABA). Taken together, our findings provide new insights into the cytological and proteomic responses of mitochondria to hypoxia during fruit storage.

3.
Plant Cell Rep ; 2022 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-34982198

RESUMO

KEY MESSAGE: CitWRKY28 and CitNAC029 are involved in cuticular wax synthesis as indicated by the comparative analysis of fruit aliphatic wax content between Citrus reticulata and Citrus trifoliata and gene co-expression analysis. Cuticular wax covers the fruit surface, playing important roles in reduction of fruit water loss and resistance to pathogen invasion. However, there is limited research on the synthesis and transcriptional regulation of cuticular wax in citrus fruit. In this study, we characterized the variations of aliphatic wax in HJ (Citrus reticulata) and ZK (Citrus trifoliata) from young fruit to mature fruit, as well as performed transcriptome sequencing on 27 samples at different fruit developmental stages. The results revealed that the ZK fruit always had a higher aliphatic wax content than the HJ fruit during development. qRT-PCR analysis demonstrated that two KCS genes, CitKCS1 and CitKCS12, had the most significant difference in expression between HJ and ZK. Furthermore, a heterologous expression assay in Arabidopsis indicated that CitKCS1 and CitKCS12 are involved in cuticular wax synthesis. Subsequently, gene co-expression network analysis screened CitWRKY28 and CitNAC029. Dual luciferase and EMSA assays indicated that CitWRKY28 might bind to the promoter of CitKCS1 and CitKCS12 and CitNAC029 might bind to that of CitKCS1 to activate their expression. Moreover, CitWRKY28 and CitNAC029 could promote the accumulation of cuticular wax in Arabidopsis leaves. Our findings provide new insights into the synthesis and regulation of cuticular wax and valuable information for further mining of wax-related genes in citrus fruit.

4.
Hematology ; 27(1): 80-87, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34964431

RESUMO

OBJECTIVES: Hemophilic arthropathy is the most common complication of severe hemophilia A. This study aims to investigated joint status and related risk factors in patients with severe hemophilia A (PWSHA). METHODS: This single-center study included 31 patients. Six index joints (both elbows, knees, and ankles) were evaluated using the Hemophilia Early Arthropathy Detection with UltraSound in China (HEAD-US-C) and Hemophilia Joint Health Score (HJHS). Treatment adherence was measured using the Validated Hemophilia Regimen Treatment Adherence Scale-Prophylaxis (VERITAS-Pro). We analyzed the influence of age, treatment delay (the interval between diagnosis and the initiation of treatment), prophylaxis, and treatment adherence on joint outcomes. RESULTS: All patients were male (median age, 22 years). The median age at diagnosis was 1 year; that at initial treatment was 5 years. All patients experienced joint bleeding. HEAD-US-C and HJHS scores were positively correlated (R = 0.70, P < 0.0001). Median [range] HEAD-US-C and HJHS scores were 15 [0-36] and 32 [2-49], respectively. Age was positively correlated with both HEAD-US-C (P = 0.002) and HJHS scores (P < 0.0001). The difference of HEAD-US-C scores between groups with ≤1 year and >1 year treatment delay was close to significant (P = 0.055). HJHS scores were significantly different between these two groups (P = 0.03). Joint assessment scores were not significantly different between on-demand and low-dose prophylaxis groups. VERITAS-Pro scores were correlated with both HEAD-US-C and HJHS scores (P = 0.046 and P = 0.005, respectively). CONCLUSIONS: Hemophilic arthropathy was pervasive in PWSHA. Age and poor adherence were significantly correlated with joint damage. Prompt treatment and adherence improvement may reduce severity.

5.
Biosens Bioelectron ; 195: 113630, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34536724

RESUMO

Nitric oxide as a signal molecule participates in a variety of physiological and pathological processes but its real-time detection in cell assays still faces challenging because of the trace amount, short half-life and easy conversion to other substances. We report here a rational design by assembling highly π-conjugated and small capacitive gaphdiyne (GDY) with a coordination complex of hemin (HEM) into a molecularly assembled material of GDY/HEM to achieve ultrafast and real-time monitoring of nitric oxide in cell assays. GDY comprising alkynyl C atoms can hybridize with the HEM to enable strong π-π interaction and atomic dispersion of iron sites while avoiding the formation of catalytically inactive dimer for the HEM. These characteristics make the GDY/HEM an excellent sensing material towards nitric oxide, which has an ultrafast response time of 0.95 s, a low detection limit of 7 nM and long linear range up to 151.38 µΜ. The GDY/HEM realizes real-time monitoring nitric oxide released from cancer and normal cells, demonstrating its capability for cell analysis.


Assuntos
Técnicas Biossensoriais , Grafite , Hemina , Óxido Nítrico
6.
Methods Mol Biol ; 2390: 1-59, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-34731463

RESUMO

Artificial intelligence (AI) has undergone rapid development in recent years and has been successfully applied to real-world problems such as drug design. In this chapter, we review recent applications of AI to problems in drug design including virtual screening, computer-aided synthesis planning, and de novo molecule generation, with a focus on the limitations of the application of AI therein and opportunities for improvement. Furthermore, we discuss the broader challenges imposed by AI in translating theoretical practice to real-world drug design; including quantifying prediction uncertainty and explaining model behavior.


Assuntos
Inteligência Artificial , Desenho de Fármacos
7.
J Colloid Interface Sci ; 608(Pt 1): 893-902, 2022 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-34785464

RESUMO

Responsive wormlike micelles (WLMs) consisted of cationic surfactants and organic-acids are fascinating due to their reversible molecular recognition properties. However, it is unknown how the structure of organic-acids alters the stimuli-responsiveness of WLMs systems. Herein, the peculiar nature of temperature-responsive behaviors in three WLMs systems were systematically investigated. These were manufactured by combining N-erucamidopropyl-N,N-dimethylamine (UC22AMPM) with isomers of organic-acids: o-phthalic acid (o-PA), m-phthalic acid (m-PA) and p-phthalic acid (p-PA) at molar ratio of 2:1 (named as o-EAPA, m-EAPA and p-EAPA respectively). The phase behaviors, macro- and micro-rheology, as well as the mechanism of temperature-responsiveness were explored by visual inspection, rheological and optical methods. The results showed that the three systems exhibited different responsiveness with increase of temperature. Among them, the viscosity and viscoelasticity of o-EAPA were gradually decreased with temperature increase from 30 °C to 90 °C. On the other hand, those of p-EAPA were firstly increased and subsequently decreased, exhibiting the highest viscosity during the heating process. This peculiar phenomenon was attributed to the hydrophilic difference of organic-acids isomers, leading to variations of micelle transitions upon temperature increase. This study is the first report of aromatic-acids isomers inducing different on temperature-responsiveness, and finding beneficial for the development of responsive WLMs for different applications.


Assuntos
Micelas , Tensoativos , Reologia , Temperatura , Viscosidade
8.
J Control Release ; 341: 443-456, 2021 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-34748870

RESUMO

Bone tissues are the main metastatic sites of many cancers, and bone metastasis is an important cause of death. When bone metastasis occurs, dynamic interactions between tumor cells and bone tissues promote changes in the tumor-bone microenvironments that are conducive to tumor growth and progression, which also promote several related diseases, including pathological fracture, bone pain, and hypercalcemia. Accordingly, it has obvious clinical benefits for improving the cure rate and reducing the occurrence of related diseases through targeting bone microenvironments for the treatment and early detection of cancer bone metastasis niches. In this review, we briefly analyzed the relationship between bone microstructures and tumor metastasis, as well as microenvironmental changes in osteoblasts, osteoclasts, immune cells, and extracellular and bone matrixes caused when metastatic tumor cells colonize bones. We also discuss novel designs in nanodrugs for inhibiting tumor proliferation and migration through targeting to tumor bone metastases and abnormal bone-microenvironment components. In addition, related researches on the early detection of bone and multi-organ metastases by nanoprobes are also introduced. And we look forward to providing some useful proposals and enlightenments on nanotechnology-based drug delivery and probes for the treatment and early detection of bone metastasis.

9.
Adv Colloid Interface Sci ; : 102527, 2021 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-34607652

RESUMO

Microemulsion, because of its excellent interfacial tension reduction and solubilization properties, has wide range of applications in the petroleum industry, especially in improved oil recovery (IOR). Herein, the concept, types and formation mechanism of microemulsion were primarily introduced. Then, the preparation and characterization methods were illustrated. Additionally, several effect factors were elaborated specifically based on the composition of microemulsion. Finally, the application of microemulsion in IOR was addressed, including IOR mechanism analysis based on sweep efficiency and displacement efficiency, injection method (microemulsion flooding, in-situ microemulsion formation) and field tests. Furthermore, the current challenges and prospects of microemulsion on IOR were analyzed.

10.
J Exp Bot ; 2021 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-34599807

RESUMO

A citrus R2R3 MYB transcription factor (CsMYB96) was found to alleviate water loss by simultaneously regulating plasma membrane intrinsic proteins (CsPIPs) and wax-related genes. Expression profiling indicated that CsPIP1;1 and CsPIP2;4 are representative aquaporins with high expression, and are down-regulated in the peel of postharvest citrus fruit. CsPIP2;4 was further characterized as the predominant CsPIP with high expression and high-water channel activity. Besides, transient overexpression of CsPIP2;4 accelerated the water loss of citrus fruit. The in silico analysis further revealed that the expression of CsMYB96 had a significant negative correlation with that of CsPIPs. In vivo and in vitro experiments confirmed that CsMYB96 can directly repress the expression of CsPIPs. Furthermore, CsMYB96 can activate the wax-related genes and promote wax biosynthesis for defense against water loss. The transient and stable overexpression of CsMYB96 reduced the water loss of citrus fruit and Arabidopsis.

11.
Materials (Basel) ; 14(20)2021 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-34683578

RESUMO

Hot compression experiments of annealed 7075 Al alloy were performed on TA DIL805D at different temperatures (733, 693, 653, 613 and 573 K) with different strain rates (1.0, 0.1, 0.01 and 0.001 s-1.) Based on experimental data, the strain-compensated Arrhenius model (SCAM) and the back-propagation artificial neural network model (BP-ANN) were constructed for the prediction of the flow stress. The predictive power of the two models was estimated by residual analysis, correlation coefficient (R) and average absolute relative error (AARE). The results reveal that the deformation parameters including strain, strain rate, and temperature have a significant effect on the flow stress of the alloy. Compared with the SCAM model, the flow stress predicted by the BP-ANN model is in better agreement with experimental values. For the BP-ANN model, the maximum residual is only 1 MPa, while it is as high as 8 MPa for the SCAM model. The R and AARE for the SCAM model are 0.9967 and 3.26%, while their values for the BP-ANN model are 0.99998 and 0.18%, respectively. All these reflect that the BP-ANN model has more accurate prediction ability than the SCAM model, which can be applied to predict the flow stress of the alloy under high temperature deformation.

12.
Nano Lett ; 21(19): 7905-7912, 2021 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-34582219

RESUMO

We demonstrate the ability to fabricate vertically stacked Si quantum dots (QDs) within SiGe nanowires with QD diameters down to 2 nm. These QDs are formed during high-temperature dry oxidation of Si/SiGe heterostructure pillars, during which Ge diffuses along the pillars' sidewalls and encapsulates the Si layers. Continued oxidation results in QDs with sizes dependent on oxidation time. The formation of a Ge-rich shell that encapsulates the Si QDs is observed, a configuration which is confirmed to be thermodynamically favorable with molecular dynamics and density functional theory. The type-II band alignment of the Si dot/SiGe pillar suggests that charge trapping on the Si QDs is possible, and electron energy loss spectra show that a conduction band offset of at least 200 meV is maintained for even the smallest Si QDs. Our approach is compatible with current Si-based manufacturing processes, offering a new avenue for realizing Si QD devices.

13.
Angew Chem Int Ed Engl ; 60(50): 26233-26237, 2021 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-34586693

RESUMO

With ever-increasing energy consumption and continuous rise in atmospheric CO2 concentration, electrochemical reduction of CO2 into chemicals/fuels is becoming a promising yet challenging solution. Sn-based materials are identified as attractive electrocatalysts for the CO2 reduction reaction (CO2 RR) to formate but suffer from insufficient selectivity and activity, especially at large cathodic current densities. Herein, we demonstrate that Cu-doped SnS2 nanoflowers can undergo in situ dynamic restructuring to generate catalytically active S-doped Cu/Sn alloy for highly selective electrochemical CO2 RR to formate over a wide potential window. Theoretical thermodynamic analysis of reaction energetics indicates that the optimal electronic structure of the Sn active site can be regulated by both S-doping and Cu-alloying to favor formate formation, while the CO and H2 pathways will be suppressed. Our findings provide a rational strategy for electronic modulation of metal active site(s) for the design of active and selective electrocatalysts towards CO2 RR.

14.
Nano Lett ; 21(18): 7753-7760, 2021 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-34516143

RESUMO

Tuning intermediate adsorption energy by shifting the d-band center offers a powerful strategy to tailor the reactivity of metal catalysts. Here we report a potential sweep method to grow Pd layer-by-layer on Au with the capability to in situ measure the surface structure through an ethanol oxidation reaction. Spectroscopic characterizations reveal charge-transfer induced valence band restructuring in the Pd overlayer, which shifts the d-band center away from the Fermi level compared to bulk Pd. Precise overlayer control gives the optimal bimetallic surface of two monolayers (ML) Pd on Au, which exhibits more than 370-fold mass activity enhancement in oxygen reduction reaction (at 0.9 V vs. reversible hydrogen electrode) and 40 mV increase in half-wave potential compared to the Pt/C. Tested in a homemade Zn-air battery, the 2-ML-Pd/Au/C exhibits a maximum power density of 296 mW/cm2 and specific activity of 804 mAh/gZn, much higher than Pt/C with the same catalyst loading amount.

15.
Nat Neurosci ; 24(10): 1402-1413, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34373644

RESUMO

Pain decreases the activity of many ventral tegmental area (VTA) dopamine (DA) neurons, yet the underlying neural circuitry connecting nociception and the DA system is not understood. Here we show that a subpopulation of lateral parabrachial (LPB) neurons is critical for relaying nociceptive signals from the spinal cord to the substantia nigra pars reticulata (SNR). SNR-projecting LPB neurons are activated by noxious stimuli and silencing them blocks pain responses in two different models of pain. LPB-targeted and nociception-recipient SNR neurons regulate VTA DA activity directly through feed-forward inhibition and indirectly by inhibiting a distinct subpopulation of VTA-projecting LPB neurons thereby reducing excitatory drive onto VTA DA neurons. Correspondingly, ablation of SNR-projecting LPB neurons is sufficient to reduce pain-mediated inhibition of DA release in vivo. The identification of a neural circuit conveying nociceptive input to DA neurons is critical to our understanding of how pain influences learning and behavior.


Assuntos
Neurônios Dopaminérgicos , Mesencéfalo/fisiopatologia , Vias Neurais/fisiopatologia , Dor/fisiopatologia , Núcleos Parabraquiais/fisiopatologia , Medula Espinal/fisiopatologia , Animais , Comportamento Animal , Mapeamento Encefálico , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Neurônios , Nociceptividade , Optogenética , Dor/psicologia , Manejo da Dor , Substância Negra/fisiopatologia , Área Tegmentar Ventral/fisiopatologia
16.
Artigo em Inglês | MEDLINE | ID: mdl-34360018

RESUMO

Nonpoint source pollution (NPS) has become the leading factor of global water quality problems, attracting great attention from governments and researchers in various countries. Based on this situation, understanding the current research status of NPS can help guide future research. However, most of the current reviews only describe the research status of some specific aspects but fail to quantify the research hotspots and development trends on the whole, which limits the overall understanding of NPS. In this paper, bibliometrics was used to study the current status, hotspots, and frontiers of NPS research during 1991-2015, and the future research development was predicted. Over the past 15 years, there has been a remarkable growth trend in publication output, and the participation of countries/territories has also increased. Journal of Environmental Quality, Journal of Hydrology, and Total Environmental Science were the top three journals. Sharpley AN and Arnold JG from the USA were the most productive authors with the best quality articles. The major author clusters and research regions are located in North America and Europe, followed by East Asia. The United States dominates this research field, with the largest number of independent and collaborative articles. Chinese authors gained more attention through international cooperation. Keyword analysis confirmed that water quality and nutrients were the main concerns of NPS pollution research, which mainly involved a number of research topics, such as pollutant emission reduction research and the evaluation and simulation of pollutants' migration and their transformation under different situations, while pesticides were less of a concern, which suggests that the abuse of pesticides has come under control. Meanwhile, SWAT was the dominating model in the last decade partly because it satisfied the growing needs of watershed-scale management.


Assuntos
Poluentes Ambientais , Poluição Difusa , Bibliometria , Europa (Continente) , Previsões
17.
J Plant Physiol ; 264: 153472, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34315028

RESUMO

Citrus fruit are generally confronted with various fungal diseases that cause fruit deterioration and economic loss. Salicylic acid (SA), a plant hormone, is an important signal molecule required for stimulating the disease resistance of plants. However, there has been limited information about the molecular mechanism of SA biosynthesis involving biotic stress response in citrus fruit. In the present study, an R2R3 MYB transcription factor (CsMYB96) was identified to mediate SA signaling in response to fungal diseases. The transient overexpression assay revealed that CsMYB96 contributed to the strong tolerance of citrus fruit to Penicillium italicum along with an increase in SA content; meanwhile, CsMYB96 conferred resistance to Botrytis cinerea in Arabidopsis plants. Further metabolomic profiling of stable transgenic Arabidopsis revealed that CsMYB96 participated in the regulation of various metabolism pathways and enhanced the accumulation of phenolic acids. RNA-seq analysis confirmed that overexpression of CsMYB96 activated the expression of genes involved in plant-pathogen interaction, phenylpropanoid biosynthesis, and SA signaling. Besides, CsMBY96 directly activated the transcription of calmodulin binding protein 60g (CsCBP60g), a predominant transcription factor required for the activation of SA signaling. In summary, our results reveal that CsMYB96 promotes SA biosynthesis and the accumulation of defense metabolites to enhance the fungal pathogen resistance of citrus fruit and Arabidopsis and provide new insights into the regulation of disease response.


Assuntos
Citrus sinensis/imunologia , Resistência à Doença , Frutas/microbiologia , Doenças das Plantas/imunologia , Reguladores de Crescimento de Plantas/metabolismo , Proteínas de Plantas/metabolismo , Ácido Salicílico/metabolismo , Fatores de Transcrição/metabolismo , Arabidopsis , Botrytis , Citrus sinensis/metabolismo , Citrus sinensis/microbiologia , Frutas/imunologia , Frutas/metabolismo , Doenças das Plantas/microbiologia , Plantas Geneticamente Modificadas
18.
Plant Sci ; 310: 110972, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34315590

RESUMO

Cuticular wax covers the surface of fleshy fruit and plays a protective role in fruit development and postharvest storage, including reducing fruit water loss, resisting biotic and abiotic stress and affecting fruit glossiness. The ß-ketoacyl-CoA synthase (KCS) is the rate-limiting enzyme of very long chain fatty acids (VLCFAs) synthesis, which provides precursors for the synthesis of cuticular wax. In this study, a total of 96 KCS genes were identified in six Citrinae species, including 13, 16, 21, 14, 16 and 16 KCS genes in the primitive species (Atalantia buxifolia), the wild species (Citrus ichangensis), and four cultivated species (Citrus medica, Citrus grandis, Citrus sinensis and Citrus clementina), respectively. Compared with primitive species, wild and cultivated species showed expansion of KCS gene family. Evolutionary analysis of KCS gene family indicated that uneven gain and loss of genes resulted in variable numbers of KCS genes in Citrinae, and KCS genes have undergone purifying selection. Expression profiles in C. sinensis revealed that the KCS genes had diverse expression patterns among various tissues. Furthermore, CsKCS2 and CsKCS11 were predominantly expressed in the flavedo and their expression increased sharply with ripening. Subcellular localization analysis indicated that CsKCS2 and CsKCS11 were located in the endoplasmic reticulum. Further, heterologous expression of CsKCS2 and CsKCS11 in Arabidopsis significantly increased the content of cuticular wax in leaves. Thus, CsKCS2 and CsKCS11 are involved in the accumulation of fruit cuticular wax at ripening. This work will facilitate further functional verification and understanding of the evolution of KCS genes in Citrinae.


Assuntos
Frutas/metabolismo , Folhas de Planta/metabolismo , Proteínas de Plantas/metabolismo , 3-Oxoacil-(Proteína de Transporte de Acila) Sintase/genética , 3-Oxoacil-(Proteína de Transporte de Acila) Sintase/metabolismo , Citrus/genética , Citrus/metabolismo , Frutas/genética , Regulação da Expressão Gênica de Plantas/genética , Regulação da Expressão Gênica de Plantas/fisiologia , Folhas de Planta/genética , Proteínas de Plantas/genética , Ceras/metabolismo
19.
Plant Mol Biol ; 106(4-5): 449-462, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34173150

RESUMO

KEY MESSAGE: The ER or donut-like structures localized aquaporin NIP5;1, which interacts with PIPs and alters their localization from plasma membrane to donut-like structures, regulates water permeability. NOD26-like intrinsic proteins (NIPs) play important roles in nutrient uptake and response to various stresses. However, there have been few studies of their functions in water transportation in citrus. Here, we demonstrate the functions of a novel citrus NIP aquaporin (CsNIP5;1) via multiple physiological and biochemical experiments. CsNIP5;1 showed high water permeability when expressed in Xenopus laevis oocytes and yeast. However, subcellular localization assays showed that this protein was localized in the endoplasmic reticulum (ER) or donut-like structures in citrus callus and tobacco leaf. Meanwhile, overexpression of CsNIP5;1 led to a reduction in the water permeability of citrus callus. Protein-protein interaction experiments and subcellular localization assays further revealed that CsNIP5;1 physically interacted with PIPs (CsPIP1;1 and AtPIP2;1), which altered their subcellular localization from the plasma membrane to donut-like structures. Together, CsNIP5;1 was identified as a good water channel when expressed in oocytes and yeast. Meanwhile, CsNIP5;1 participated in the regulation of water permeability of citrus callus, which may be associated with CsNIP5;1-induced re-localization of water channels PIPs. In summary, these results provide new insights into the regulatory mechanism of AQPs-mediated water diffusion.


Assuntos
Aquaporina 1/metabolismo , Permeabilidade da Membrana Celular/fisiologia , Membrana Celular/metabolismo , Citrus/metabolismo , Proteínas de Plantas/metabolismo , Água/metabolismo , Animais , Retículo Endoplasmático/metabolismo , Xenopus laevis
20.
J Food Sci ; 86(7): 3023-3032, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34146407

RESUMO

Kiwifruit contains abundant nutritive compounds and is highly favored by the consumers worldwide. Therefore, detailed metabolic profiling is important to provide theoretic basis for the improvement of kiwifruit quality. In this study, the levels of volatiles, carotenoids, and mineral elements in the flesh of 17 kiwifruit accessions were evaluated. Acids and esters were the main volatiles in kiwifruit. During these 17 kiwifruit accessions, "Chenhong," three "Jinyan," and two "Guichang" germplasms were specifically rich in aromatic esters, which might be associated with their special taste. The main carotenoids were lutein, ß-carotene, and zeaxanthin, and their levels were also genotype specific, with the green-fleshed "Guichang" having the highest level of carotenoids, and red-fleshed "Fuhong" and "Chenhong" being rich in zeaxanthin. Partial correlation analysis showed that the contents of some mineral elements were significantly correlated with those of specific volatiles and carotenoids, indicating the impacts of mineral elements on the accumulation of volatiles and carotenoids in the kiwifruit flesh. These results indicated that the contents of carotenoids and volatiles seemed to be affected by mineral elements and also provided a new potential method for improving fruit flavor quality in production.


Assuntos
Actinidia/metabolismo , Carotenoides/química , Frutas/química , Minerais/química , Actinidia/química , Actinidia/classificação , Actinidia/genética , Carotenoides/metabolismo , Frutas/classificação , Frutas/genética , Frutas/metabolismo , Genótipo , Minerais/metabolismo , Volatilização
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