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1.
Biomacromolecules ; 2020 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-33175509

RESUMO

Pathogenic bacteria adhesion and formation of biofilm on the implant are the most common reasons for healthcare-associated device failure. Cationic amphiphilic polymer brushes containing covalently linked quaternary ammonium salts (QASs) are considered to be the most promising bactericidal materials, but these surfaces still suffer from incomplete bactericidal ability and serious microorganism accumulation. With this in mind, a novel kind of hierarchical surface integrating both geminized cationic amphiphilic antibacterial upper layer and zwitterionic antifouling sublayer has been developed in this study. Measurements of X-ray photoelectron spectroscopy, spectroscopic ellipsometry, atomic force microscopy, water contact angle, and surface ζ-potential were performed to investigate the surface functionalization process. The thicknesses and grafting densities of the pAGC8 upper blocks have been optimized to avert the mutual interference among different components. The optimal hierarchical surface exhibits an ultrahigh antibacterial activity and a potent self-cleaning functionality against both Staphylococcus aureus and Escherichia coli bacteria, as well as a certain protein repellence ability. Such a novel hierarchical architecture provides innovative guidance for the construction of super-antibacterial and self-cleaning brushes in many biomedical applications.

2.
Artigo em Inglês | MEDLINE | ID: mdl-33142013

RESUMO

Synthetic nucleic acids, with four non-canonical nucleobases, can function as genetic materials. A comprehensive analysis of PCR amplification, transcription, reverse transcription, and cloning was done to screen for alternative genetic monomers. A small library of six modified nucleobases was selected: the modified 2'-deoxyribonucleoside (dZTPs) and ribonucleoside (rZTPs) triphosphates of 7-deaza-adenine, 5-chlorouracil, 7-deaza-guanine or inosine together with 5-fluorocytosine or 5-bromocytosine. The fragments composed of one to four modified nucleotides (denoted as DZA) have been successfully recognised and transcribed to natural or modified RNA (denoted as RZA) by T7 RNA polymerase. The fully modified RZA fragment could be reverse transcribed and then amplified in the presence of various dZTPs. Noticeably, modified fragments could function as genetic templates in vivo by encoding the 678 base pair gene of a fluorescent protein in bacteria. These results demonstrate the existence of a fully simulated genetic circuit that uses synthetic materials.

3.
Int J Older People Nurs ; : e12355, 2020 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-33135323

RESUMO

BACKGROUND: Physical inactivity exposes older adults living in long-term care institutions to a high risk of health deterioration. Developing effective behaviour change interventions based on a theoretical framework is a pressing concern. AIMS: This study developed an 'Easy Walking' intervention programme based on the Wheel of Motivation and aimed to: (1) develop the intervention programme for promoting self-efficacy of older adults living in long-term care facilities; and (2) examine the perceived helpfulness of the intervention programme for encouraging walking behaviours. METHODS: This study consisted of two stages. In the first stage, a three-round modified Delphi process was conducted with ten experts to rate the eight motivators in the Wheel of Motivation. The Easy Walking programme was designed accordingly. In the second stage, a single-group pretest-posttest study design was employed to evaluate the Easy Walking programme. Structured questionnaires were used to collect data on the changes in self-efficacy and on the perceived helpfulness regarding the programme. RESULTS: The Easy Walking intervention programme features eight factors that influence motivation. Thirty older adults participated in and evaluated the programme. The results showed a significant difference in self-efficacy (t = -7.02, p < .001) of the older adults. Regarding the perceived usefulness of the intervention, the mean scores for each item ranged from 3.73 to 4.93 points. 'Safe environment' was perceived to be the most helpful factor for encouraging walking behaviours. CONCLUSION: The Easy Walking programme enhanced the self-efficacy of institutionalised older adults and was perceived as helpful in physical activity engagement. Nursing professionals in long-term care institutions could implement the Easy Walking programme to be part of daily nursing activities.

4.
Behav Brain Funct ; 16(1): 10, 2020 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-33208152

RESUMO

OBJECTIVE: The purpose of this study was to evaluate the effect of astaxanthin (AST) on cognition function, inflammatory response and oxidative stress in vascular dementia (VD) mice. METHOD: VD mice model was established by left unilateral common carotid arteries occlusion (LUCCAO). Following LUCCAO, AST was intragastrically administered for 30 days. Object recognition test and morris water maze test were used to evaluate cognitive function. Hematoxylin and eosin staining was performed to observe the hippocampal neuron structure. Enzyme-linked immunosorbent assay kit and bicinchoninic acid kit were respectively adopted to measure IL-1ß and IL-4 protein expression and superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in hippocampus and prefrontal cortex. RESULTS: AST improved the discrimination ability of VD mice. The escape latency and path length of VD mice treated with AST were dramatically reduced. Besides, AST 200 mg/kg enhanced crossing platform time and the number of times crossing the platform quadrant, and alleviated the morphological impairment in VD mice. Moreover, we found that AST inhibited IL-1ß expression and MDA content, whereas promoted IL-4 expression and SOD activity in a dose-dependent manner. CONCLUSION: AST could improve cognitive impairment and hippocampal neurons in VD mice, which may be related to suppression of inflammatory response and oxidative stress.

5.
Environ Pollut ; 268(Pt A): 115847, 2020 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-33130443

RESUMO

Cadmium (Cd) pollution is currently the most serious type of heavy metal pollution throughout the world. Previous studies have shown that Cd elevates the mortality of paddy field spiders, but the lethal mechanism remains to be explored profoundly. In the present study, we measured the activities of protective enzymes (acetylcholinesterase, glutathione peroxidase, phenol oxidase) and a heavy metal chelating protein (metallothionein) in the pond wolf spider Pardosa pseudoannulata after Cd exposure. The results indicated that Cd initially increased the enzyme activities and protein concentration of the spider after 10- and 20-day exposure before inhibiting them at 30-day exposure. Further analysis showed that the enzyme activities in the cephalothorax were inhibited to some extent. Since the cephalothorax region contains important venom glands, we performed transcriptome sequencing (RNA-seq) analysis of the venom glands collected from the spiders after long-term Cd exposure. RNA-seq yielded a total of 2826 differentially expressed genes (DEGs), and most of the DEGs were annotated into the process of protein synthesis, processing and degradation. Furthermore, a mass of genes involved in protein recognition and endoplasmic reticulum (ER) -associated protein degradation were down-regulated. The reduction of protease activities supports the view that protein synthesis and degradation in organelles and cytoplasm were dramatically inhibited. Collectively, our outcomes illustrate that Cd poses adverse effects on the expression of protective enzymes and protein, which potentially down-regulates the immune function in the venom glands of the spiders via the alteration of protein processing and degradation in the ER.

6.
Analyst ; 145(23): 7551-7558, 2020 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-33140759

RESUMO

Extracellular vesicles (EVs) represent an important mode of intercellular communication in both disease and developmental biology, exposing their potential in diagnostics and therapeutics. Recently, aptamer-based sensors, i.e. aptasensors, have been gradually applied in EV analysis due to their high selectivity and sensitivity. A fluorescent aptasensor enables easy readout by flow cytometry (FCM) and has more accuracy and convenience than conventional immunoassays for EV analysis. Here, we develop a fluorescent aptasensor-based method for quantitative analysis of nano-sized membrane vesicles by using high-resolution FCM. EVs as small as 100 nm are detected and quantified using a dual-staining procedure with the fluorescent aptasensor targeting CD63 and a cytoplasmic dye. Nano-sized EVs derived from bone marrow mesenchymal stem cells, human neural stem cells and human cornea epithelial cells are analyzed, and the result shows that their amount varies from 6.79 × 106 mL-1 to 2.08 × 108 mL-1 in culture media. The technique is also used to evaluate the bioactivity of EVs and, in the future, it may develop into a versatile tool to analyze and quantify EVs from a variety of biological objects with conventional cytometric instruments.

7.
Artigo em Inglês | MEDLINE | ID: mdl-33223398

RESUMO

BACKGROUND & AIMS: Over the last few decades, the prevalence of metabolic syndrome (MetS) has gradually increased. As we know, many prior studies have connected MetS with diabetes, coronary heart disease, and cardiovascular disease. Abdominal aortic calcification (AAC) is a good marker of morbidity and mortality of vascular disease, as its degree may be associated with the severity of coronary artery calcification and disease. The aim of this article is to investigate the connection between MetS and AAC. METHODS AND RESULTS: This retrospective observational study included 2731 participants aged 58 years from the National Health and Nutrition Examination Survey (NHANES) (2013-2014). We used Dual-Energy X-ray Absorptiometry to define the degree of AAC. We defined MetS according to the National Cholesterol Education Program Adult Treatment Panel III definition. A total of 2731 participants with complete data were included for data analysis. In the fully adjusted model, an increase in the severity of AAC with the number of MetS components was still significant with ßvalues of AAC Total 24 Score 0.498 (95% confidence interval (CI): 0.018,0.978), 1.016 (95% CI: 0.514,1.519) and 1.426 (95% CI: 0.916,1.937) respectively in 2, 3 and â‰§ 4 components. Additionally, associations were observed between MetS components, including blood pressure, HDL and glucose with ßvalues of AAC Total 24 Score 0.332(95% CI: 0.069, 0.595), 0.652(95% CI: 0.380, 0.925) and 0.534 (95% CI: 0.285, 0.783) after fully adjusted, respectively. CONCLUSION: The results indicated that, in the US adult population, a greater number of components of MetS were significantly associated with AAC. Among the components of metabolic syndrome, the blood pressure, HDL and blood sugar were observed apparent association with AAC.

8.
Sci Rep ; 10(1): 20186, 2020 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-33214634

RESUMO

Obstructive sleep apnea syndrome (OSAS) can lead to alterations in right ventricular (RV) performance and pulmonary vascular haemodynamics. Additionally, altitude-related hypoxia is associated with pulmonary vasoconstriction, and the effect of high-altitude on the pulmonary circulation in OSAS patients can be further altered. We sought to assess alterations in RV morphology and function in OSAS patients living at high altitude by way of 2-dimensional speckle tracking echocardiography (2D-STE), real-time 3- dimensional echocardiography (RT-3DE) and cardiac biomarkers. We also evaluate the impact of continuous positive airway pressure (CPAP) treatment on RV performance. Seventy-one patients with newly diagnosed OSAS and thirty-one controls were included in this study. All individuals were assessed for cardiac biomarkers as well as underwent 2D-STE and RT-3DE. Forty-five OSAS patients underwent CPAP therapy for at least 24 weeks and were studied before and after CPAP treatment. RT-3DE was used to measure RV volume, and calculate RV 3D ejection fraction (3D RVEF). Peak systolic strain was determined. Cardiac biomarkers, including C-reactive protein (CRP), N-terminal pro-B-type natriuretic peptide, and cardiac troponin T were also measured. Right atrium volume index, RV volume, RV volume index, systolic pulmonary artery pressure (sPAP), pulmonary vascular resistance (PVR) and level of serum CRP were significantly higher in OSAS group, while OSAS patients showed lower 3D RVEF and RV longitudinal strains. Compared to the patients with sPAP < 40 mmHg, RV longitudinal strains in patients with sPAP ≥ 40 mmHg were lower. Both RV global longitudinal strain and sPAP were associated with apnea-hypopnea index. Patients treated with 6 months of CPAP therapy had significant improvement in RV geometry and performance. RV structural abnormalities and RV function impairments were observed in OSAS patients living at moderate high altitude compared to control highlanders. The reversibility of these changes after application of CPAP were further confirmed.

9.
Adv Mater ; : e2004401, 2020 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-33169447

RESUMO

Solar evaporation through a photothermal porous material provides a feasible and sustainable method for water remediation. Several photothermal materials have been developed to enhance solar evaporation efficiency. However, a critical limitation of current photothermal materials is their inability to separate water from the volatile organic compounds (VOCs) present in wastewater. Here, a microstructured ultrathin polymeric membrane that enables freshwater separation from VOC pollutants by solar evaporation with a VOC removal rate of 90%, is reported. The different solution-diffusion behaviors of water and VOCs with polymeric membranes facilitate their separation. Moreover, owing to increased light absorption, enlarged liquid-air interface, and shortened mass transfer distance, the microstructured and ultrathin configuration of the membrane helps to balance the tradeoff between permeation selectivity and water production capacity. The membrane is not only effective for evaporation of simulated volatile pollutants in a prototype, but can also intercept complex volatile organic contaminants in natural water sources and produce water that meets drinking-water standards. With practical demonstration and satisfactory purification performance, this work paves the way for practical application of solar evaporation for effective water remediation.

10.
Small ; : e2005095, 2020 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-33169496

RESUMO

Engineering intriguing electrode with exceptional kinetics behaviors is imperative for boosting sodium storage systems. Herein, the uniform nanoframes are threaded by the interwoven carbon nanotube (CNT) conductive network to form an ingenious beads-on-a-string structured NiFePBA nanoframe/CNT (NFPB-NF/CNT) cathode and the corresponding derivative NiFeSe nanoframe/CNT (NFS-NF/CNT) anode. NFPB-NF/CNT exhibited remarkable cycling life along with outstanding rate capability and low voltage decay per cycle. The fast Na+ conduction and sufficient sodiation reaction is proved using Na+ diffusion models. Meanwhile, an exceptional sodium storage capacity and prolonged cycling life is achieved using binary NFS-NF/CNT anode at a high rate. In situ and ex situ investigations are used to reveal the sodium storage mechanisms and structural evolution process. Furthermore, a sodium-ion full battery based on the above electrodes shows stable performance accompanied by high energy conversion efficiency. The material design strategy provides a new solution for exploiting smart multicomponent composites toward advanced energy storage devices.

11.
Artigo em Inglês | MEDLINE | ID: mdl-33156796

RESUMO

The Internet of Things (IoT) has propelled the evolution of medical sensing technologies to greater heights. Thus, traditional health systems have been transformed into new data-rich environments. This provides an unprecedented opportunity to develop new analytical methods and tools towards a new paradigm of smart and interconnected health systems. Nevertheless, there are risks pertinent to increasing levels of system connectivity and data accessibility. Cyber-attacks become more prevalent and complex, leading to greater likelihood of data breaches. These events bring sudden disruptions to routine operations and cause the loss of billions of dollars. Adversaries often attempt to leverage models to learn a targets sensitive attributes or extrapolate its inclusion within a database. As healthcare systems are critical to improving the wellbeing of our society, there is an urgent need to protect the privacy of patients and minimize the risk of model inversion attacks. This paper presents a new approach, named Mosaic Gradient Perturbation (MGP), to preserve privacy in the framework of predictive modeling, which meets the requirement of differential privacy while mitigating the risk of model inversion. MGP is flexible in fine-tuning the trade-offs between model performance and attack accuracy while being highly scalable for large-scale computing. Experimental results show that the proposed MGP method improves upon traditional gradient perturbation to mitigate the risk of model inversion while offering greater preservation of model accuracy. The MGP technique shows strong potential to circumvent paramount costs due to privacy breaches while maintaining the quality of existing decision-support systems, thereby ushering in a privacy-preserving smart health system.

12.
J Transl Med ; 18(1): 432, 2020 11 12.
Artigo em Inglês | MEDLINE | ID: mdl-33183315

RESUMO

Extracellular vesicles are heterogeneous populations of naturally occurring secreted small vesicles. EVs function as signaling platforms to facilitate intracellular communication, which indicates the physiological or pathophysiological conditions of cells or tissues. Considering that EVs can be isolated from most body fluids and that molecular constituents could be reprogrammed according to the physiological status of the secreting cells, EVs are regarded as novel diagnostic and prognostic biomarkers for many diseases. The ability to protect encapsulated molecules from degradation in body fluids suggests the potential of EVs as biological medicines or drug delivery systems. This article focuses on the EV-associated biomarkers and therapeutic approaches in autoimmune diseases.

13.
Biomed Res Int ; 2020: 9689386, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33204730

RESUMO

Sericin is a natural protein component of silks of silkworm and has potential utility in multiple areas such as pharmacological, cosmetics, and biotechnological industries. However, the understanding of its toxicological safety is still limited. This study evaluated the safety of water-extract sericin from silkworm (Bombyx mori) cocoons using different model approaches, including three genotoxicity studies (the bacterial reverse mutation test, the mammalian erythrocyte micronucleus test, and the mouse spermatogonia chromosomal aberration test) and a 90-day subchronic toxicity study in Sprague-Dawley (SD) rats. The results of this study showed that water-extract sericin was nonmutagenic and nongenotoxic both in vitro and in vivo. Sericin did not induce significant changes in the body and organ weight, food intake, blood hematology and serum biochemistry, urine index, and histopathology in rats. The NOAEL of sericin was determined to be 1 g/kg/day for male and female rats. These results indicated that water-extract sericin was of low toxicity in the experimental conditions of the current study and had the potential for application in food-related products.

14.
Sci Total Environ ; : 143333, 2020 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-33190884

RESUMO

Carbon materials derived from metal organic frameworks (MOFs) have excellent properties of high surface area, high porosity, adjustable pore size, high conductivity and stability, and their applications in catalysis have become a rapidly expanding research field. In this review, we have summarized the synthesis strategies of MOF-derived carbons with different physical and chemical properties, obtained through direct carbonization, co-pyrolysis and post-treatment. The potential applications of derived carbons, especially monometal-, bimetal-, nonmetal-doped and metal-free carbons in organo-catalysis, photocatalysis and electrocatalysis are analyzed in detail from the environmental perspective. In addition, the improvement of catalytic efficiency is also considered from the aspects of increasing active sites, enhancing the activity of reactants and promoting free electron transfer. The function and synergy of various species of the composites in the catalytic reaction are summarized. The reaction paths and mechanisms are analyzed, and research ideas or trends are proposed for further development.

15.
J Formos Med Assoc ; 2020 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-33191095

RESUMO

BACKGROUND/PURPOSE: Oral submucous fibrosis (OSF) is an irreversible fibrosis disease and a potentially malignant disorder in the oral cavity. Various studies have shown that miR-21 was implicated in the fibrogenesis and carcinogenesis, but its functional role in the development of OSF has not been investigated. METHODS: The expression levels of miR-21 in arecoline-stimulated normal buccal mucosal fibroblasts (BMFs) and OSF specimens were determined by qRT-PCR. Exogenous administration of TGF-ß and its inhibitor (SB431542) were utilized to examine the involvement of TGF-ß signaling in miR-21 alteration. Collagen gel contraction, transwell migration, and invasion assays were used to assess the myofibroblast activities. The relationship between α-SMA and miR-21 was calculated using the Pearson correlation coefficient. RESULTS: MiR-21 expression was induced in BMFs by arecoline treatment in a dose-dependent manner. Our results showed that this upregulation was mediated by TGF-ß signaling. Subsequently, we demonstrated that the administration of the miR-21 inhibitor suppressed the arecoline-induced myofibroblast characteristics, including a higher collagen gel contractility and cell motility, in normal BMFs. Furthermore, inhibition of miR-21 was sufficient to attenuate the myofibroblast features in fibrotic BMFs. Besides, we showed that the expression of miR-21 was aberrantly upregulated in the OSF tissues and there was a positive correlation between miR-21 and myofibroblast marker, α-SMA. CONCLUSION: MiR-21 overexpression in OSF may be due to the stimulation of areca nut, which was mediated by the TGF-ß pathway. Our data suggested that the repression of miR-21 was a promising direction to palliate the development and progression of OSF.

16.
Nanoscale ; 2020 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-33211786

RESUMO

Rational materials design for the synthesis of desirable hollow micro- and nanostructures has recently revealed the remarkable potential for high-performance energy storage and conversion devices. Owing to their unique "core-void-shell" structural configurations, yolk-shell-structured electrode materials can achieve intimate contact with the electrolyte and alleviate the volume expansion issue during electrochemical cycling, which is therefore poised to further boost the electrochemical properties of hybrid supercapacitors. Herein, a facile self-templated strategy, consisting of a hydrothermal step and a high-temperature sulfurization process, has been developed for the construction of yolk-shell (NiCo)9S8 spheres in situ coated by graphite carbon ((NiCo)9S8/GC) due to the non-equilibrium thermal treatment of alkali metal alkoxides. The as-synthesized yolk-shelled sphere exhibits a high specific capacitance of 1434.4 F g-1 (179.3 mA h g-1) at a current density of 1 A g-1, and good rate capability and cycling stability with 83.1% capacitance retention at 8 A g-1 over 5000 cycles. To further demonstrate its practical application, a hybrid supercapacitor device was assembled using (NiCo)9S8/GC as the battery-type positive electrode and activated carbon (AC) as the capacitive-type electrode. The as-fabricated device can reach a wide voltage window of up to 1.6 V, deliver a high energy density of 55.6 W h kg-1 at a power density of 800.3 W kg-1 and maintain 90.2% of specific capacitance after 3000 cycles.

17.
Nanoscale ; 2020 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-33185635

RESUMO

Effective ion intercalation nanomaterials provide tremendous opportunities to various deionization systems such as capacitive deionization (CDI) to significantly improve the removal capacity of brackish water desalination. However, the asymmetric design of CDI devices causes a low removal rate due to the indispensable regeneration half-cycle. Furthermore, choices of chloride selective electrodes for such devices are limited. This imposes a big challenge on further improvement of CDI systems. Herein, we report a cation-selective CDI system using a single bi-functional Na2VTi(PO4)3@carbon nanomaterial with redox couples of V4+/V3+ and Ti3+/Ti4+ as an advanced symmetric electrode. The as-prepared continuous desalination set-up shows a superior removal rate of 0.022 mg g-1 s-1 (1.32 mg g-1 min-1) with a high half-cycle removal capacity of 35 mg g-1, and extremely low energy consumption of 0.14 W h g-1 (at a current density of 100 mA g-1). In addition, an extremely high cycle-stability of at least 50 cycles is achieved. The bi-functional intercalation mechanism is investigated by in situ XRD and ex situ XPS. The symmetric device yields a simplified and low-cost configuration with improved energy efficiency and high removal capacity. This opens a new horizon towards the commercialization of CDI technologies.

18.
Oncol Rep ; 44(6): 2503-2516, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33125127

RESUMO

Aberrant methylation is one of the most frequent epigenetic alterations that regulate the expression levels of genes, including long non­coding RNAs (lncRNAs), in tumors. However, to the best of our knowledge, the expression and function of hepatic nuclear factor 1α antisense RNA 1 (HNF1A­AS1) and its methylation condition have not yet been reported in the development and progression of laryngeal squamous cell carcinoma (LSCC). In the present study, the expression and methylation of HNF1A­AS1 were first examined by reverse transcription­quantitative PCR, bisulfite genomic sequencing and methylation­specific polymerase chain reaction in samples from patients with LSCC, which were based on the in silico analysis using The Cancer Genome Atlas data, and were then further verified in LSCC cell lines with and without 5­Aza­2'­deoxycytidine (5­Aza­dC) treatment. Subsequently, proliferation, cell cycle distribution, migration and invasion of LSCC cells following either knockdown or overexpression of HNF1A­AS1 were determined in vitro. Furthermore, the characteristic of HNF1A­AS1 on epithelial­mesenchymal transition (EMT) changes was investigated in vitro and in vivo. The associations between the expression levels of HNF1A­AS1 and tumorigenicity and cervical lymph node metastasis were assessed in a xenograft model in nude mice. In the present study, downregulation and hypermethylation in CpG sites of HNF1A­AS1 were detected in LSCC tissues as well as metastatic cervical lymph nodes samples when compared with those in the adjacent non­tumor tissues. Additionally, HNF1A­AS1 inhibited proliferation, migration and invasion of LSCC cells in vitro by regulating the process of EMT. Furthermore, HNF1A­AS1 inhibited tumor growth and metastasis by regulating EMT in vivo. Additionally, the migration and invasion abilities, and the expression levels of HNF1A­AS1 and EMT markers in LSCC cells were significantly reversed by treatment with 5­Aza­dC. In summary, HNF1A­AS1 was downregulated by hypermethylation in LSCC and laryngeal cancer cells. These findings suggested that HNF1A­AS1 could serve as a tumor suppressor lncRNA in LSCC by regulating the EMT process, leading to the discovery of novel therapeutic targets and strategies for the treatment of patients with LSCC.

19.
BMC Med Genet ; 21(1): 199, 2020 10 09.
Artigo em Inglês | MEDLINE | ID: mdl-33036557

RESUMO

BACKGROUND: This case-control study aims to examine the association between the Interleukin-6 (IL-6) rs12700386 polymorphism and the increased risk of developing osteoarthritis (OA) in the knee in the Chinese Han population. METHODS: We extracted DNA from 763 subjects (352 OA patients and 411 healthy controls). The relative expression levels of IL-6 in blood samples of patients with knee OA was determined by quantitative reverse transcription PCR (qRT-PCR) and polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) was used for genotyping the IL-6 gene polymorphism. RESULTS: We found that the IL-6 polymorphism rs12700386 enhanced patient susceptibility to developing knee OA. Based on a subgroup analysis, the risk of developing knee OA was elevated in smokers, drinkers, and subjects ≥55 years old or with BMI ≥ 25 kg/m2. The combination of smoking, drinking, and having the rs12700386 genotype led to an increase in the risk of developing knee OA, indicating that an underlying interaction between gene and environment exists. The rs12700386 genotype was found to be correlated with an increase in IL-6 expression. We also found that IL-6 levels were significantly higher in the CC genotype compared to the GG genotype carriers in OA patients. CONCLUSION: These data suggest that the rs12700386 polymorphism in the IL-6 gene leads to an increase in the risk of knee OA in Chinese Han individuals.


Assuntos
Predisposição Genética para Doença/genética , Interleucina-6/genética , Osteoartrite do Joelho/genética , Polimorfismo de Nucleotídeo Único , Idoso , Grupo com Ancestrais do Continente Asiático/genética , Estudos de Casos e Controles , China , Feminino , Frequência do Gene , Predisposição Genética para Doença/etnologia , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Osteoartrite do Joelho/etnologia , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição
20.
J Mech Behav Biomed Mater ; 113: 104125, 2020 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-33068923

RESUMO

This study aimed to evaluate the effects of repolishing on the surface microhardness (SMH), color change (ΔE), and translucency parameter (TP) of previously in situ eroded computer-aided design/computer-aided manufacturing (CAD/CAM) restorative materials and human enamel. Each of 8 volunteers wore an intraoral appliance containing 3 CAD/CAM restorative material specimens (IPS e.max CAD lithium disilicate ceramic, Lava Ultimate hybrid ceramic, and poly (methyl methacrylate) (PMMA) block) and 1 human enamel specimen. The specimens were subjected to in situ erosion cycles by rinsing with a cola drink (4 × 5 min/day) for 14 days. After erosion, the specimens were polished with a silicone polishing system (Ceramister, Shofu Inc, Kyoto, Japan). The SMH and color of the specimens were determined at baseline (T1), after erosion (T2), and after repolishing (T3). The ΔE and TP values of the specimens were further calculated. The data were statistically analyzed using repeated-measures analysis of variance (ANOVA) and Bonferroni's test (α = 0.05). After erosion, a decrease in the SMH of the restorative materials and enamel was observed (all P < 0.001), and a decrease in the TP of the enamel was observed (P = 0.016). The ΔE values of the enamel (ΔE = 7.32) and Lava Ultimate (ΔE = 3.19) exceeded the clinically unacceptable threshold after erosion. After repolishing, the SMH of the restorative materials and enamel at T3 was significantly higher than that at T2 (all P < 0.001). No significant difference was found in the TP and ΔE values of the restorative materials and enamel between T2 and T3. In conclusion, erosion negatively affected the surface properties and appearance of the CAD/CAM restorative materials and human enamel. Repolishing contributed to restoring the compromised SMH of the eroded restorative materials and enamel to a certain extent. However, repolishing did not restore the color of the eroded restorative materials and enamel.

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