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1.
Cereb Cortex ; 2021 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-34642754

RESUMO

Emotion dysregulation is one of the core features of major depressive disorder (MDD). However, most studies in depression have focused on unimodal emotion processing, whereas emotional perception in daily life is highly dependent on multimodal sensory inputs. Here, we proposed a novel multilevel discriminative framework to identify the altered neural patterns in processing audiovisual emotion in MDD. Seventy-four participants underwent an audiovisual emotional task functional magnetic resonance imaging scanning. Three levels of whole-brain functional features were extracted for each subject, including the task-evoked activation, task-modulated connectivity, combined activation and connectivity. Support vector machine classification and prediction models were built to identify MDD from controls and evaluate clinical relevance. We revealed that complex neural networks including the emotion regulation network (prefrontal areas and limbic-subcortical regions) and the multisensory integration network (lateral temporal cortex and motor areas) had the discriminative power. Moreover, by integrating comprehensive information of local and interactive processes, multilevel models could lead to a substantial increase in classification accuracy and depression severity prediction. Together, we highlight the high representational capacity of machine learning algorithms to characterize the complex network abnormalities associated with emotional regulation and multisensory integration in MDD. These findings provide novel evidence for the neural mechanisms underlying multimodal emotion dysregulation of depression.

2.
Stem Cell Res Ther ; 12(1): 555, 2021 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-34717753

RESUMO

BACKGROUND: Cell therapy provides hope for treatment of advanced liver failure. Proliferating human hepatocytes (ProliHHs) were derived from primary human hepatocytes (PHH) and as potential alternative for cell therapy in liver diseases. Due to the continuous decline of mature hepatic genes and increase of progenitor like genes during ProliHHs expanding, it is challenge to monitor the critical changes of the whole process. Raman microspectroscopy is a noninvasive, label free analytical technique with high sensitivity capacity. In this study, we evaluated the potential and feasibility to identify ProliHHs from PHH with Raman spectroscopy. METHODS: Raman spectra were collected at least 600 single spectrum for PHH and ProliHHs at different stages (Passage 1 to Passage 4). Linear discriminant analysis and a two-layer machine learning model were used to analyze the Raman spectroscopy data. Significant differences in Raman bands were validated by the associated conventional kits. RESULTS: Linear discriminant analysis successfully classified ProliHHs at different stages and PHH. A two-layer machine learning model was established and the overall accuracy was at 84.6%. Significant differences in Raman bands have been found within different ProliHHs cell groups, especially changes at 1003 cm-1, 1206 cm-1 and 1440 cm-1. These changes were linked with reactive oxygen species, hydroxyproline and triglyceride levels in ProliHHs, and the hypothesis were consistent with the corresponding assay results. CONCLUSIONS: In brief, Raman spectroscopy was successfully employed to identify different stages of ProliHHs during dedifferentiation process. The approach can simultaneously trace multiple changes of cellular components from somatic cells to progenitor cells.

3.
ACS Chem Biol ; 16(11): 2560-2569, 2021 11 19.
Artigo em Inglês | MEDLINE | ID: mdl-34618427

RESUMO

Histone posttranslational modifications (PTMs) are vital epigenetic regulators in many fundamental cell signaling pathways and diverse biological processes. Histone lysine benzoylation is a recently identified epigenetic mark associated with active transcription; however, it remains to be explored. Herein, we first report the genetic encoding of benzoyllysine and fluorinated benzoyllysines into full-length histone proteins in a site-specific manner in live cells, based on our rationally designed synthetase and fine-integrated fluorine element into benzoyllysines. The incorporated unnatural amino acids integrating unique features were demonstrated as versatile probes for investigating histone benzoylation under biological environments, conferring multiplex signals such as 19F NMR spectra with chemical clarity and fluorescence signals for benzoylation. Moreover, the site specifically incorporated lysine benzoylation within native full-length histone proteins revealed distinct dynamics of debenzoylation in the presence of debenzoylase sirtuin 2 (SIRT2). Our developed strategy for genetic encoding of benzoyllysines offers a general and novel approach to gain insights into interactions of site-specific histone benzoylation modifications with interactomes and molecular mechanisms in physiological settings, which could not be accessible with fragment histone peptides. This versatile chemical tool enables a direct and new avenue to explore benzoylation, interactions, and histone epigenetics, which will provide broad utilities in chemical biology, protein science, and basic biology research.

4.
Brain Topogr ; 34(6): 779-792, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34480635

RESUMO

Integrating multimodal information into a unified perception is a fundamental human capacity. McGurk effect is a remarkable multisensory illusion that demonstrates a percept different from incongruent auditory and visual syllables. However, not all listeners perceive the McGurk illusion to the same degree. The neural basis for individual differences in modulation of multisensory integration and syllabic perception remains largely unclear. To probe the possible involvement of specific neural circuits in individual differences in multisensory speech perception, we first implemented a behavioral experiment to examine the McGurk susceptibility. Then, functional magnetic resonance imaging was performed in 63 participants to measure the brain activity in response to non-McGurk audiovisual syllables. We revealed significant individual variability in McGurk illusion perception. Moreover, we found significant differential activations of the auditory and visual regions and the left Superior temporal sulcus (STS), as well as multiple motor areas between strong and weak McGurk perceivers. Importantly, the individual engagement of the STS and motor areas could specifically predict the behavioral McGurk susceptibility, contrary to the sensory regions. These findings suggest that the distinct multimodal integration in STS as well as coordinated phonemic modulatory processes in motor circuits may serve as a neural substrate for interindividual differences in multisensory speech perception.

5.
Front Microbiol ; 12: 673872, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34354679

RESUMO

Human norovirus is a common cause of acute gastroenteritis worldwide, and oysters have been found to be the main carriers for its spread. The lack of efficient pre-treatment methods has been a major bottleneck limiting the detection of viruses in oysters. In this study, we established a novel immunomagnetic enrichment method using polyamidoamine (PAMAM) dendrimer/SA-biotin-mediated cascade amplification for reverse transcriptase quantitative real-time polymerase chain reaction (RT-qPCR) detection. We compared the capture efficiency of traditional immunomagnetic enrichment, biotin-amplified immunomagnetic enrichment, and PAMAM dendrimer/SA-biotin-mediated cascade-amplification immunomagnetic enrichment. The optimal capture efficiency of the novel method was 44.26 ± 1.45%, which increased by 183.17% (P < 0.01) and 18.09% (P < 0.05) compared with the first two methods, respectively. Three methods were all applied in detecting norovirus in 44 retail oysters, the detection rate of the PAMAM dendrimer/SA-biotin-mediated method was 25.0%, which was higher than those of traditional IME (15.90%) and SA-biotin-amplified IME (18.80%) by 9.1 and 6.2%, respectively. In conclusion, the novel method can be applied for the rapid detection of norovirus in oysters, which can help reduce the cost and time of detection and improve detection rates.

6.
Cell Rep ; 36(3): 109411, 2021 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-34289348

RESUMO

Oxytocin is a well-known neurohypophysial hormone that plays an important role in behavioral anxiety and nociception. Two major forms of long-term potentiation, presynaptic LTP (pre-LTP) and postsynaptic LTP (post-LTP), have been characterized in the anterior cingulate cortex (ACC). Both pre-LTP and post-LTP contribute to chronic-pain-related anxiety and behavioral sensitization. The roles of oxytocin in the ACC have not been studied. Here, we find that microinjections of oxytocin into the ACC attenuate nociceptive responses and anxiety-like behavioral responses in animals with neuropathic pain. Application of oxytocin selectively blocks the maintenance of pre-LTP but not post-LTP. In addition, oxytocin enhances inhibitory transmission and excites ACC interneurons. Similar results are obtained by using selective optical stimulation of oxytocin-containing projecting terminals in the ACC in animals with neuropathic pain. Our results demonstrate that oxytocin acts on central synapses and reduces chronic-pain-induced anxiety by reducing pre-LTP.

7.
J Agric Food Chem ; 69(23): 6485-6494, 2021 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-34077226

RESUMO

Silver nanoparticles (AgNPs) have remarkable and broad-spectrum antibacterial activities against Gram-positive (G+) and Gram-negative bacteria (G-). However, the negative surface potential of AgNPs limits their antibacterial activities due to the electrostatic repulsion with the negatively charged bacterial cell membrane. To address the limitation, AgNPs were loaded in the mesoporous silica nanoparticles by preparing silver core-mesoporous silica shell nanocapsules (Ag@MSNs), and then, a cationic antibacterial polymer, quaternary ammonium polyethyleneimine (QPEI), was used to modify Ag@MSNs for improving their surface potential and antibacterial activities. The results showed that the obtained Ag@MSN-QPEI exhibited a high positive surface potential (+39.6 mV) and a strong electrostatic attraction with Pseudomonas syringae pv. lachrymans cells in coculture, resulting in an excellent bacterial cell-targeting effect. At the same concentration, Ag@MSN-QPEI exhibited less silver content (reducing the silver content of Ag@MSNs by 19%), higher antibacterial activities, and longer effective duration against Clavibacter michiganensis subsp. michiganensis (G+) and P. syringae pv. lachrymans (G-) than Ag@MSNs and QPEI alone. The excellent bacterial cell-targeting effect and synergistic antibacterial action combined with QPEI accounted for the significantly enhanced antibacterial activities of Ag@MSN-QPEI. Therefore, using a cationic antibacterial polymer to confer the bacterial cell-targeting effect and synergistic antibacterial action would be extended to other antimicrobial materials.


Assuntos
Compostos de Amônio , Nanopartículas Metálicas , Nanocápsulas , Antibacterianos/farmacologia , Testes de Sensibilidade Microbiana , Polietilenoimina , Dióxido de Silício , Prata/farmacologia
8.
Curr Med Sci ; 41(3): 572-580, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-34047945

RESUMO

The exact mechanism by which knockout of Toll-like receptor 4 (TLR4) attenuates the liver injury remains unclear. The present study aimed to examine the role of TLR4 in the pathogenesis of bile duct ligation (BDL)-induced liver cholestatic injury and the underlying mechanism. Wild type (WT) mice and TLR4 knockout (TLR4-KO) mice were used for the establishment of the BDL model. Metabolomics were applied to analyze the changes of small molecular metabolites in the serum and liver of the two groups. The serum biochemical indexes and the HE staining results of liver tissue showed that liver damage was significantly reduced in TLR4-KO mice after BDL when compared with that in WT mice. The metabolite analysis results showed that TLR4 KO could maintain the metabolisms of amino acids- and choline-related metabolites. After BDL, the amino acids- and choline-related metabolites, especially choline and 3-hydroxybutyrate, were significantly increased in WT mice (both in serum and liver), but these metabolites in the liver of TLR4-KO mice after BLD were not significant different from those before BLD. In conclusion, TLR4 KO could attenuate BDL-induced liver cholestatic injury through regulating amino acid and choline metabolic pathways.

9.
Front Microbiol ; 12: 653719, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33889144

RESUMO

Human norovirus is regarded as the leading cause of epidemic acute gastroenteritis with GII.4 being the predominant genotype during the past decades. In the winter of 2014/2015, the GII.17 Kawasaki 2014 emerged as the predominant genotype, surpassing GII.4 in several East Asian countries. Hence, the influence of host immunity response on the continuous evolution of different GII.17 variants needs to be studied in depth. Here, we relate the inferences of evolutionary mechanisms of different GII.17 variants with the investigation of cross-reactivity and cross-protection of their respective antisera using the expression of norovirus P particles in Escherichia coli. The cross-reactivity assay showed that the antisera of previous strains (GII.17 A and GII.17 B) reacted with recent variants (GII.17 C and GII.17 D) at high OD values from 0.8 to 1.16, while recent variant antisera cross-reacting with previous strains were weak with OD values between 0.26 and 0.56. The cross-protection assay indicated that the antisera of previous strains had no inhibitory effect on recent variants. Finally, mutations at amino acids 353-363, 373-384, 394-404, and 444-454 had the greatest impact on cross-reactivity. These data indicate that the recent pandemic variants GII.17 C and GII.17 D avoided the herd immunity effect of previous GII.17 A and GII.17 B strains through antigenic variation.

10.
ACS Appl Mater Interfaces ; 13(17): 20114-20124, 2021 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-33896182

RESUMO

g-C3N4 with π-delocalization was coordinated between urea and a small amount of 1,3,5-tris(4-aminophenyl)benzene (TAPB) (UCN-xTAPB) by a facile polymerization. Compared with pristine g-C3N4(UCN), the obtained materials, UCN-xTAPB, showed an extended delocalization with increased electrical conductivity, enhanced adsorption of visible light, and improved separation of photogenerated electron-hole pairs. The average H2 evolution rate of UCN-4TAPB is about 10.55 mmol h-1 g-1 under visible-light irradiation (λ > 420 nm), which is much higher than reported data. Furthermore, density-functional theory (DFT) calculation confirms that the proposed structure with the incorporation of TAPB into the CN network shows the extended delocalization. Moreover, different structures of aromatic rings (anthroic acid, naphthoic acid and benzoic acid) are applied to verify the role of the enhanced π-delocalization in g-C3N4. By adopting different precursors (thiourea, dicyandiamide) to polymerize with TAPB, we further confirm the extension of optical absorption under visible-light irradiation and the improvement of hydrogen evolution rate, indicating the universality of the current strategy. Therefore, we believe that our work provides an efficient strategy for constructing the delocalized structure of g-C3N4 as effective visible-light-responsive photocatalysts.

11.
ACS Appl Mater Interfaces ; 13(12): 14587-14598, 2021 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-33740376

RESUMO

A high-temperature air-stable solar selective absorber (SSA) based on TiW-SiO2 cermet is prepared by the co-sputtering method. The obtained SSA shows remarkable stability in spectrum, structure, and chemistry after air-annealing at 700 °C, demonstrating its resistance against air erosion at high temperature. Comparing with W-SiO2-based SSA, the addition of the Ti element is proved to be effective in enhancing the thermal stability of SSA. Nevertheless, as the temperature increases to 750 °C, perfectly round cavities appear and induce the deterioration of the coating. A phase transformation from α-W to ß-W is found at the interface of TiW/HMVF (high metal volume fraction layer) during deposition. Consequently, the inverse phase transformation from ß-W to α-W at above 750 °C results in small vacancies at the interface, being the incentive of cavity generation. Afterward, the violent morphological changes of oxidized TiW accelerate the cavities expansion. To enhance its tolerance ability of service temperature, a Cr barrier layer is introduced to prevent the diffusion of oxygen into the TiW layer. Therefore, the optimal SSA performs stably at 800 °C and the failure temperature is elevated to 850 °C, revealing that the air-stable TiW-SiO2-based SSA has outstanding potential in high-temperature photothermal conversion.

12.
BMC Infect Dis ; 21(1): 248, 2021 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-33750333

RESUMO

BACKGROUND: Human noroviruses are one of the main causes of foodborne illnesses and represent a serious public health concern. Rapid and sensitive assays for human norovirus detection are undoubtedly necessary for clinical diagnosis, especially in regions without more sophisticated equipment. METHOD: The rapid reverse transcription recombinase-aided amplification (RT-RAA) is a fast, robust and isothermal nucleic acid detection method based on enzyme reaction. This method can complete the sample detection at 39 °C in 30 min. In this study, we successfully established a rapid reverse transcription recombinase-aided amplification (RT-RAA) assay for the detection of human norovirus GII.4 and applied this assay to clinical samples, as well as comparison with commercial reverse transcription real-time fluorescence quantitative PCR (RT-qPCR). RESULTS: At 95% probability, the detection sensitivity of RT-RAA was 3.425 log10 genomic copies (LGC)/reaction. Moreover, no cross-reaction was observed with other norovirus genogroups and other common foodborne viruses. Stool samples were examined by RT-RAA and reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). Compared of RT-qPCR, kappa values for human norovirus detection with RT-RAA were 0.894 (p < 0.001), indicating that both assays were in agreement. CONCLUSION: This RT-RAA assay provides a rapid, specific, and sensitive assay for human norovirus detection and is suitable for clinical testing.


Assuntos
Infecções por Caliciviridae/diagnóstico , Gastroenterite/diagnóstico , Norovirus/genética , Técnicas de Amplificação de Ácido Nucleico/métodos , Sequência de Bases , Infecções por Caliciviridae/virologia , Primers do DNA/metabolismo , Gastroenterite/virologia , Genótipo , Humanos , Norovirus/isolamento & purificação , RNA Viral/química , RNA Viral/genética , RNA Viral/metabolismo , Recombinases/metabolismo , Sensibilidade e Especificidade , Alinhamento de Sequência
13.
J Agric Food Chem ; 69(8): 2382-2391, 2021 Mar 03.
Artigo em Inglês | MEDLINE | ID: mdl-33605718

RESUMO

Considering the severity of plant pathogen resistance toward commonly used agricultural microbicides, as well as the potential threats of agrichemicals to the eco-environment, there is a pressing need for antimicrobial approaches that are capable of inactivating pathogens efficiently without the risk of inducing resistances and harm. In this work, a porphyrin metal-organic framework (MOF) nanocomposite was constructed by incorporating 5,10,15,20-tetrakis(1-methyl-4-pyridinio)porphyrin tetra(p-toluenesulfonate) (TMPyP) as a photosensitizer (PS) in the cage of a variant MOF (HKUST-1) to efficiently produce singlet oxygen (1O2) to inactivate plant pathogens under light irradiation. The results showed that the prepared PS@MOF had a loading rate of PS about 12% (w/w) and excellent and broad-spectrum photodynamic antimicrobial activity in vitro against three plant pathogenic fungi and two pathogenic bacteria. Moreover, PS@MOF showed outstanding control efficacy against Sclerotinia sclerotiorum on cucumber in the pot experiment. Allium cepa chromosome aberration assays and safety evaluation on cucumber and Chinese cabbage indicated that PS@MOF had no genotoxicity and was safe to plants. Thus, porphyrin MOF demonstrated a great potential as an alternative and efficient new microbicide for sustainable plant disease management.


Assuntos
Anti-Infecciosos , Estruturas Metalorgânicas , Porfirinas , Anti-Infecciosos/farmacologia , Ascomicetos , Gerenciamento Clínico , Fármacos Fotossensibilizantes/farmacologia , Porfirinas/farmacologia
14.
J Obstet Gynaecol Res ; 47(4): 1397-1408, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33470027

RESUMO

PURPOSE: To explore the risk factors, ultrasonic manifestations, clinical features, and maternal and neonatal outcomes associated with complete uterine rupture. BASIC PROCEDURES: All cases of complete uterine rupture diagnosed and treated in Jiangxi Maternal and Child Health Hospital from January 2012 to July 2018 were retrospectively analyzed. Risk factors, ultrasonic manifestations, clinical features, and maternal and infant outcomes were analyzed. RESULT: All patients had a history of uterine surgery or induced abortion. Ultrasound examination revealed 15 cases of complete rupture of the uterus, five cases of missed diagnosis, three cases of misdiagnosis, and two cases of direct emergency operation without ultrasonography because of typical clinical manifestations and critical conditions. The clinical manifestations of 25 cases of uterine rupture varied from asymptomatic to clinical signs of "resting" rupture of the uterus to severe pain, hypotension, shock, and coma. All patients underwent surgical treatment, of which one case underwent DIC and died after rescue. The maternal mortality rate was 4% (1/25), the mortality rate of newborns (two pregnant women was twins) was 44% (12/27). CONCLUSION: A history of uterine surgery is a major risk factor for uterine rupture. Attention should be paid not only to women who are pregnant again after cesarean section but also to those who have undergone other uterine operations (such as laparoscopic myomectomy, laparoscopic cornual pregnancy removal, etc.), delivery plans should be formulated accordingly. In cases of sudden abdominal pain during pregnancy or childbirth, the possibility of uterine rupture should be considered to achieve a timely and correct diagnosis and treatment.


Assuntos
Ruptura Uterina , Cesárea/efeitos adversos , Criança , Feminino , Humanos , Recém-Nascido , Mortalidade Materna , Gravidez , Resultado da Gravidez , Estudos Retrospectivos , Ultrassom , Ruptura Uterina/diagnóstico por imagem , Ruptura Uterina/epidemiologia , Ruptura Uterina/etiologia
15.
Plant Physiol Biochem ; 159: 268-276, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33401201

RESUMO

Quercetin is one of the main flavonoids in the human diet and mainly found in different plant tissues, including seeds, flowers, leaves, stems, and roots. However, its biological function in plant tissues, especially in seeds, is unknown. In this study, the seed germination and subsequent seedling growth of Apocynum pictum and A. venetum under osmotic stress (400 mmol L-1 mannitol) supplemented with 5 µmol L-1 quercetin were evaluated after 7, 14, and 21 days of germination. Results showed that quercetin improved the germination percentage and seed vigor, as indicated by the higher germination energy, shoot length, root length, dry weight, fresh weight, and chlorophyll content in A. pictum and A. venetum seedlings under the mannitol compared with those under the mannitol alone. Quercetin decreased H2O2 and O2- production and cell membrane damage, and mostly increased the gene expression of superoxide dismutase, peroxidase, catalase, chalcone synthase and flavonol synthase in A. pictum and A. venetum seedlings under the mannitol compared with those under the mannitol alone. In addition, the germination energy of A. pictum was 21.57% higher than that of A. venetum, and the gene expression of key enzymes in quercetin biosynthesis in A. pictum was mostly higher than that in A. venetum after 1 and 7 days of germination. These results indicated that quercetin was an effective anti-osmotic agent that alleviated the adverse effect of mannitol-induced osmotic stress on seed germination and seed vigor, and A. pictum seeds were more osmotic resistant than A. venetum seeds.


Assuntos
Apocynum , Germinação , Manitol , Pressão Osmótica , Quercetina , Apocynum/efeitos dos fármacos , Apocynum/crescimento & desenvolvimento , Germinação/efeitos dos fármacos , Peróxido de Hidrogênio , Manitol/farmacologia , Pressão Osmótica/efeitos dos fármacos , Quercetina/farmacologia , Plântula/efeitos dos fármacos , Plântula/crescimento & desenvolvimento , Sementes/efeitos dos fármacos , Sementes/crescimento & desenvolvimento
16.
Front Psychol ; 11: 558871, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33192817

RESUMO

Little is known about the time of development of binocular suppression. In the present study, we evaluated the emergence of binocular suppression in infants by using continuous flash suppression (CFS, Tsuchiya and Koch, 2005). In our experiment, one eye of infants was presented with a static face image at one side of the screen, while another eye was presented with dynamic Mondrian patterns in full screen. Adult observers confirmed that the static face image was consciously repressed by the changing Mondrian patterns. If binocular suppression was functional, the infants would not perceive the face and thus would not show any preference in the experiment. However, if binocular suppression in the infants was not yet acquired, they would perceive the face and the Mondrian patterns at the same time and would thus show preference for the side where the face was presented. The results showed that infants aged 2-3 months, but not those aged 4-5 months, detected the position of the face. Furthermore, this detection was not due to weak contrast sensitivity to the dynamic Mondrian mask. These results indicated that the immature binocular visual system may perceive different images from different eyes simultaneously and that infants may lose this ability after establishing binocular suppression at 4-5 months of age.

17.
Exp Cell Res ; 396(2): 112299, 2020 11 15.
Artigo em Inglês | MEDLINE | ID: mdl-32979365

RESUMO

OBJECTIVES: Congenital cystic adenomatoid malformation (CCAM) is the most common congenital pulmonary anomaly with unknown etiology. Here, single-cell RNA sequencing (scRNA-seq) was used to map its cellular landscape and identify the underlying cellular and molecular events related to CCAM. METHODS: This study involved a 4.25 year old patient with grade Ⅱ-Ⅲ CCAM at the Children's Hospital of Fudan University. Samples of lesioned and non-lesioned areas were collected during surgery for scRNA-seq. RESULTS: In total, 19,904 cells were obtained with median UMI counts of 7032 per cell and 1995 median genes per cell. In terms of lesioned and non-lesioned areas, epithelial cells accounted for 27.23% and 17.85%, respectively, while mesenchymal cells accounted for 2.67% and 16.06%, respectively (P < 0.0001). Further clustering of epithelial cells revealed that the fractions of alveolar type 1 cells (AT1, N: 23.65%; L: 49.81%), AT2(N: 2.02%; L: 5.26%), club-1(N: 9.02%; L: 17.57%), club-3(N: 1.18%; L: 4.15%), and basal cells (N: 0.34%; L: 2.93%) were increased in lesioned samples (P < 0.0001). Pseudotime trajectory analysis showed tracks of club-1/basal cells→AT2→club-3→AT1 and club-1,2/basal→AT2. Mast cells (N: 0.63%; L: 2.48%) were also increased in lesioned samples and interactions of CD44 with HBEGF and FGFR2 were detected between mast and epithelial cells. CONCLUSIONS: AT1, AT2, club, and basal cells were increased in CCAM patients, and newly defined club-1/3 and basal cells might be the origin of proliferating AT1 and AT2 cells. Increased mast cells might promote epithelial cell proliferation through interactions of CD44 with HBEGF and FGFR2.


Assuntos
Malformação Adenomatoide Cística Congênita do Pulmão/genética , Malformação Adenomatoide Cística Congênita do Pulmão/patologia , Células Epiteliais/metabolismo , Células Epiteliais/patologia , Perfilação da Expressão Gênica , Análise de Célula Única , Células Epiteliais Alveolares/metabolismo , Células Epiteliais Alveolares/patologia , Linhagem da Célula/genética , Proliferação de Células/genética , Pré-Escolar , Humanos , Pulmão/metabolismo , Pulmão/patologia , Mastócitos/metabolismo
18.
J Neural Eng ; 17(5): 056013, 2020 10 13.
Artigo em Inglês | MEDLINE | ID: mdl-32906091

RESUMO

OBJECTIVE: Visual perception decoding plays an important role in understanding our visual systems. Recent functional magnetic resonance imaging (fMRI) studies have made great advances in predicting the visual content of the single stimulus from the evoked response. In this work, we proposed a novel framework to extend previous works by simultaneously decoding the temporal and category information of visual stimuli from fMRI activities. APPROACH: 3 T fMRI data of five volunteers were acquired while they were viewing five categories of natural images with random presentation intervals. For each subject, we trained two classification-based decoding modules that were used to identify the occurrence time and semantic categories of the visual stimuli. In each module, we adopted recurrent neural network (RNN), which has proven to be highly effective for learning nonlinear representations from sequential data, for the analysis of the temporal dynamics of fMRI activity patterns. Finally, we integrated the two modules into a complete framework. MAIN RESULTS: The proposed framework shows promising decoding performance. The average decoding accuracy across five subjects was over 19 times the chance level. Moreover, we compared the decoding performance of the early visual cortex (eVC) and the high-level visual cortex (hVC). The comparison results indicated that both eVC and hVC participated in processing visual stimuli, but the semantic information of the visual stimuli was mainly represented in hVC. SIGNIFICANCE: The proposed framework advances the decoding of visual experiences and facilitates a better understanding of our visual functions.


Assuntos
Imageamento por Ressonância Magnética , Córtex Visual , Mapeamento Encefálico , Humanos , Redes Neurais de Computação , Córtex Visual/diagnóstico por imagem , Percepção Visual
19.
Front Oncol ; 10: 1104, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32793474

RESUMO

Accumulated evidences suggested that circular RNAs (circRNA) played critical roles in tumorigenesis and progression. To our knowledge, no study reported the function of circular RNA DGKB (circDGKB, circRNA ID: hsa_circ_0133622) on progression of neuroblastoma (NB). Here, we showed that circDGKB was upregulated in NB tissues compared to the normal dorsal root ganglia. Moreover, the expression level of circDGKB was negatively correlated with the survival rate of NB patients. Mechanically, overexpression of circDGKB promoted the proliferation, migration, invasion, and tumorigenesis of NB cells and reduced cell apoptosis, and vice versa. In addition, qRT-PCR and/or Western blot results showed that circDGKB overexpression inhibited the expression level of miR-873 and enhanced GLI1 expression. Moreover, miR-873 functioned an opposite role to circDGKB and significantly weakened circDGKB role in promoting NB progression. Furthermore, GLI1 upregulation also rescued the miR-873 role in inhibiting NB progression. In conclusion, our work proved that circDGKB promoted NB progression via targeting miR-873/GLI1 axis in vitro and in vivo. Our study provided a new target for NB treatment and indicated that circDGKB could act as a novel diagnostic marker for NB.

20.
J Pediatr Surg ; 55(12): 2766-2771, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32829882

RESUMO

PURPOSE: To explore the surgical treatment and predictors of intestinal necrosis in children with intestinal obstruction through analyzing blood biochemical indicators, and to establish a predictive model and evaluate its predictive accuracy, sensitivity and specificity. METHODS: The data of children with intestinal obstruction hospitalized in Jiangxi Provincial Children's Hospital from January 2014 to June 2019 were retrospectively analyzed. RESULTS: Thirty-six substances in the blood of children with successful conservative management and those requiring surgical treatment were significantly different. The model composed of 7 variables, including age, white blood cell count, creatine kinase, troponin I, myoglobin, C-reactive protein and fibrinogen, can be used to predict the unsuccessful conservative management in children with intestinal obstruction, whom need further operation. The average prediction accuracy was 83.50%, the false positive rate was 16.67% (32/192), AUROC is 0.9160 (95% CI, 0.8930-0.9390), and the sensitivity and specificity were 83.20% and 92.70% respectively. A prediction model based on the white blood cell count, creatine kinase, troponin I and myoglobin could predict the occurrence of intestinal necrosis. The average prediction accuracy was 73.70%, false positive rate was 4.49% (15/334), AUROC was 0.7390 (95% CI, 0.6820-0.7960), and the sensitivity and specificity were 71.70% and 64.70%, respectively. CONCLUSIONS: Combination of age, white blood cell count, creatine kinase, troponin I, myoglobin, C-reactive protein and fibrinogen can be used to predict whether the children with intestinal obstruction need surgical treatment or not. Leukocyte count, creatine kinase, troponin I and myoglobin are closely related to the condition of children with intestinal obstruction and can be used to predict whether intestinal necrosis occurs. TYPE OF STUDY: Retrospective Study LEVELS OF EVIDENCE: Level I.


Assuntos
Obstrução Intestinal , Intestinos/patologia , Biomarcadores/sangue , Criança , Humanos , Obstrução Intestinal/cirurgia , Intestinos/cirurgia , Necrose , Estudos Retrospectivos , Sensibilidade e Especificidade
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