Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 2.906
Filtrar
1.
J Hazard Mater ; 383: 121169, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31520931

RESUMO

Nickel (Ni) contamination in soils, at high concentrations, is considered to be very common. Knowledge of the total content of Ni is frequently insufficient to estimate environmental risk. Our explored findings showed that the earthworms adding reduced the available Ni, along with the superior performance of HCl than CaCl2. The bioaccumulation of Ni in earthworms was aggravated with increasing Ni dosage and exposure time. Bioaccumulation factor was significantly correlated with the extractable Ni, which was the most suitable predicting the variations of Ni bioavailability. LC50 of earthworms on 7 and 14 days were 1202.444 mg kg-1 and 1069.324 mg kg-1, respectively along with the recovery rate in 500 mg kg-1 Ni polluted soil reached up to 92.5%. Earthworms' respiration was sensitive presenting a significant dose-effect relationship with the Ni concentration. Five biochemical indices in earthworms were induced along with the relevance of a dose- and time-response pattern. Additionally, histological damage in earthworm's body wall, intestine and seminal vesicles were observed under high level of Ni exposure. Overall, we believe that our current study will open a new window for deeper insights into the potential availability of Ni along with other associated metals on the function of soil ecosystem.

2.
Carbohydr Polym ; 228: 115359, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31635738

RESUMO

This study investigated the chain conformation, physicochemical properties of fucosylated chondroitin sulfate from Stichopus chloronotus (fCS-Sc) using HPSEC-MALLS and worm-like cylinder model methods and its impact on human gut microbiota by 16s RNA gene sequencing. The results indicated that fCS-Sc adopted rigid rods chain conformation in PBS based on chain conformational parameters αη (1.11) and αh (0.70). Stiffness parameters deduced from worm-like cylinder model suggested that fCS-Sc exhibited as stiff chains. The congo red assay indicated a single-helical structure. fCS-Sc demonstrated a negative zeta-potentials in a wide pH ranges and good thermal stability. Besides, fCS-Sc could modulate the community structure of gut microbiota by elevating the absolute abundance of microbiota, increasing the beneficial bacterium Megamonas, Bacteroides, Fusobacterium, Parabacteroides, Prevotella, Faecalibacterium, suppressing pathogenic microorganism and promoting the short-chain fatty acids production. The present studies improved the understanding of the spatial structures of fCSs and its application as prebiotics.

3.
Neural Netw ; 122: 117-129, 2019 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-31677440

RESUMO

Along with the explosive growing of data, semi-supervised learning attracts increasing attention in the past years due to its powerful capability in labeling unlabeled data and knowledge mining. As an emerging method, the semi-supervised extreme learning machine (SSELM), that builds on ELM, has been developed for data classification and shown superiorities in learning efficiency and accuracy. However, the optimization of SSELM as well as most of the other ELMs is generally based on the mean square error (MSE) criterion, which has been shown less effective in dealing with non-Gaussian noises. In this paper, a robust regularized correntropy criterion based SSELM (RC-SSELM) is developed. The optimization of the output weight matrix of RC-SSELM is derived by the fixed-point iteration based approach. A convergent analysis of the proposed RC-SSELM is presented based on the half-quadratic optimization technique. Experimental results on 4 synthetic datasets and 13 benchmark UCI datasets are provided to show the superiorities of the proposed RC-SSELM over SSELM and other state-of-the-art methods.

4.
Parkinsonism Relat Disord ; 69: 94-98, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31726290

RESUMO

BACKGROUND: Autosomal-dominant striatal degeneration (ADSD) is a rare neurodegenerative movement disorder caused by mutations in the Phosphodiesterase 8B (PDE8B) gene. OBJECTIVE: To summarize the clinical and imaging features of a Chinese ADSD family and determine whether mutations in PDE8B are associated with Parkinson's disease (PD) or Parkinsonism. METHODS: Clinical, imaging and genetic findings in a Chinese ADSD family are reported. Rare, potentially pathogenic variants in PDE8B were searched in whole-exome sequencing datasets from 1714 PD or parkinsonism patients and 1039 controls. RESULTS: An ADSD diagnosis was confirmed by a nonsense mutation in PDE8B (p.E102X) in a patient and a presymptomatic carrier. Clinically, the patient exhibited progressive parkinsonism without tremor and ataxia phenotype. Neuroimaging showed an inhomogeneous increased signal in the patient's striatum on T1-weighted images but a decreased signal in the presymptomatic carrier. Diffusion tensor imaging (DTI) showed a disturbance in the white matter fiber distribution, especially between the lentiform nucleus and caudate nucleus, which was more prominent in the patient than in the presymptomatic carrier. Within the 1714 patients, three PDE8B missense variants were identified that were unlikely to be the cause of the parkinsonism phenotype according to the functional prediction and mutation types reported in ADSD. CONCLUSIONS: For the first time, we described the typical ataxia phenotype in ADSD. A loss of white matter fiber integrity was shown on DTI scanning. No causative PDE8B mutation was discovered in our cohort of PD or Parkinsonism patients.

5.
Chem Commun (Camb) ; 55(96): 14458-14461, 2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31728469

RESUMO

A detailed mechanism study on the anion ligand promoted selective C-H bond fluorination is reported. The role of the anion ligand has been clarified by experimental evidence and DFT calculations. Moreover, the nitrate promoted C-F bond reductive elimination enabled a selective C-H bond fluorination of various symmetric and asymmetric azobenzenes to access diverse o-fluoroanilines.

6.
J Interv Cardiol ; 2019: 6762089, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31772542

RESUMO

Introduction: Although coronary artery disease (CAD) presentations and clinical outcomes differ by sex, little is known about premature CAD (PCAD). The present analysis aimed to evaluate the gender-related differences of PCAD in an Asian population from the FOCUS registry. Methods: A total of 1397 Asian young patients with angiographically confirmed CAD undergoing drug-eluting stent implantation were included in this analysis and divided into two groups according to the genders. Patients were followed up for three years and clinical outcomes were compared between groups. Results: Young women were older and more likely to have hypertension and diabetes than men (all p<0.001). In contrast, males with PCAD had higher BMI and higher prevalence of current smoking as well as previous vessel revascularizations (all p<0.05). Men were more likely to be manifested as total occlusive lesions (p<0.001). Regardless of the clinical characteristics, the cumulative incidences of adverse events such as major adverse cardiovascular event (MACE), cardiovascular death, and all-cause death were not significantly different at one- or three-year follow-up (all p>0.05). Conclusion: Despite remarkable differences in clinical characteristics between Asian males and females with PCAD, the two groups did not differ significantly in clinical outcomes.

7.
Breast Cancer ; 2019 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-31773503

RESUMO

PURPOSE: Anti-cancer therapy put patients in an immunocompromised status. Reactivation of cytomegalovirus (CMV) in immunocompromised patient can cause a severe disease. Thus, we presented a case who had recurrent CMV colitis which complicate with rectovaginal fistula. METHODS: We present a case of everolimus-associated cytomegalovirus colitis on a patient receiving everolimus and exemestane therapy for the treatment of metastasized breast cancer. RESULTS: The patient presented septic shock and acute peritonitis at first. Emergency exploratory laparotomy was performed. However, only edematous changes were observed over the terminal ileum, sigmoid colon and rectum. Four weeks after operation, we found feces coming out from her vagina. Colonoscopy was done and revealed rectovaginal fistula. Colonic and rectal mucosa moderate inflammation with multiple ulcer was also noted. Biopsy was done and the pathology proved CMV colitis. After treatment with ganciclovir, her symptoms improved. Everolimus was stopped for 12 weeks and was added back with a decreasing dose paradigm for breast cancer treatment. However, another episode of CMV colitis occurred again after resuming the everolimus. After anti-virus treatment, she was discharged. Due to adverse effects, everolimus therapy was discontinued. CONCLUSION: The standard treatment of hormone receptor positive and HER-2 negative metastatic breast cancer is everolimus together with exemestane. Due to the immunosuppressive effects of everolimus, the medication may cause invasive fungal infection or other opportunistic infections. Such infections are serious and may even be fatal. In this case, we did not consider CMV infection until rectovaginal fistula formation. Thus, for solid cancer patients presented with fever of unknown origin, clinicians should consider potential complications of CMV infection.

8.
Adv Exp Med Biol ; 1206: 329-357, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31776993

RESUMO

Autophagy is a lysosome-dependent catabolic process. Both extra- and intra-cellular components are engulfed in autophagic vacuoles and degraded to simple molecules, such as monosaccharides, fatty acids and amino acids. Then, these molecules can be further used to produce ATP through catabolic reactions and/or provide building blocks for the synthesis of essential proteins. Therefore, we consider autophagy a critical and fine-tuned process in maintaining energy homeostasis. The complicated relationships between autophagy and energy metabolism have raised broad interest and have been extensively studied. In this chapter, we summarize the relationships enabling autophagy to control or modulate energy metabolism and allowing metabolic pathways to regulate autophagy. Specifically, we review the correlations between autophagy and energy homeostasis in terms of oxidative phosphorylation, reactive oxygen species in mitochondria, glycolysis, metabolism of glycogen and protein, and so on. An understanding of the role of autophagy in energy homeostasis could help us better appreciate how autophagy determines cell fate under stressful conditions or pathological processes.

9.
Medicine (Baltimore) ; 98(48): e18187, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31770274

RESUMO

Currently, little is known regarding the predictive utility of aortic arch calcification (AAC) for clinical outcomes in patients with acute coronary syndrome (ACS) who undergo percutaneous coronary intervention (PCI). The present study was designed to investigate the predictive performance of AAC as detected by chest x-ray for clinical outcomes among ACS patients undergoing PCI.A total of 912 patients who were diagnosed as ACS and treated with PCI were included in this prospective, cohort study. All study participants received chest x-rays on admission, and a semiquantitative 4-point scale was used to assess the extent of AAC. The primary end point was defined as a composite of major adverse cardiovascular events (MACE) comprising death, nonfatal stroke, nonfatal myocardial infarction, and unplanned repeat revascularization. The key secondary end point was the composite of cardiovascular death, nonfatal stroke, and nonfatal myocardial infarction. The prognostic values of AAC were assessed in multivariate Cox-proportional hazards regression analyses adjusted for major confounders.The mean follow-up duration was 917 days and, during the follow-up period, MACE occurred in 168 (18.4%) patients. Kaplan-Meier analyses revealed significantly higher incidences of the primary and key secondary end points in patients with higher AAC grades (log-rank test; all P < .001). Multivariate Cox-proportional hazards regression analyses showed that, in comparison to AAC grade 0, the hazard ratios of AAC grades 1, 2, and 3 for predicting MACE were 1.63 (95% confidence interval [CI] 0.99-2.67), 2.15 (95% CI 1.27-3.62), and 2.88 (95% CI 1.41-5.86), respectively. The C-index of the variables, including peripheral arterial disease and serum levels of triglyceride for predicting MACE, was 0.644 (95% CI 0.600-0.687) versus 0.677 (95% CI 0.635-0.719) when AAC grades were also included; the continuous net reclassification improvement was 16.5% (8.7%-23.4%; P < .001).The extent of AAC as detected by chest x-ray is an independent predictor of MACE among ACS patients undergoing PCI. Further research is warranted to evaluate whether specific treatment strategies that are established based on AAC extent are needed for optimal risk reduction in relevant patient populations.

10.
J Agric Food Chem ; 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31747263

RESUMO

To improve the mechanical strength of chitosan hydrogels, gallic acid-modified carboxymethyl chitosan/iron ion (GA-CMCS/FeⅢ) complex hydrogels were initially prepared via noncovalent interactions and metal coordination. 1H NMR, 13C NMR, and Fourier transform infrared spectra analysis confirmed the covalent reactions between CMCS and GA. The formation of GA-CMCS hydrogel occurred through electrostatic interactions, hydrogen bonds, and hydrophobic interaction. GA-CMCS/FeⅢ hydrogel (380 kPa) exhibited higher compressive strength than GA-CMCS hydrogel (315 kPa). In addition, GA-CMCS/FeⅢ aerogel displayed satisfactory mechanical strength (1.59 MPa) and high-efficiency adsorption capacities up to 97.15 mg/g for Pb2+, 99.75 mg/g for Cd2+, and 98.50 mg/g for Cu2+, respectively. In addition, both of the GA-CMCS and GA-CMCS/FeⅢ aerogel adsorbents exhibited satisfactory recyclability. The design of GA-CMCS and GA-CMCS/FeⅢ hydrogels and aerogels offers a new opportunity for the construction of physically multi-crosslinked systems with excellent mechanical properties.

11.
J Immunol ; 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31748346

RESUMO

Coxsackievirus B is a common cause of viral myocarditis and pancreatitis. IL-17A is intensively involved in the pathogenesis of viral myocarditis. Whether IL-17A plays a role in Coxsackievirus B-induced pancreatitis, characterized by acinar cell destruction and immune infiltration, remains largely unknown. We found a significant, but transient, increase of IL-17A expression and γδT influx in the pancreas of C57BL/6J mice within 3 d following CVB3 infection. The pancreatic IL-17A was mainly produced by Vγ4 γδ T cells, to a lesser extent by CD4+ Th17 cells. IL-17A-/- and TCRδ-/- mice both reduced their susceptibility to CVB3 infection and pancreatitis severity when compared with the wild-type mice, without altering viral load. mAb depletion of Vγ4γδ T cells significantly improved mice survival and pancreatic pathology via decreasing Th17 expansion and neutrophil influx into the pancreas compared with isotype-treated mice. Transfer of Vγ4γδ T cells from wild-type, but not IL-17-/-, mice reconstituted TCRδ-/- mice to produce IL-17 and develop pancreatitis to the level of wild-type mice during CVB3 infection, indicating γδ T IL-17A is required for the onset of viral pancreatitis. IL-23 was robustly induced in the pancreas within the first day of infection. Administration of exogenous rIL-23 to mice increased CVB3 pancreatitis through in vivo expansion of IL-17+γδT17 cells at 12 h postinfection. Our findings reveal a key pathogenic role for early-activated γδT17 cells in viral pancreatitis via promoting neutrophil infiltration and Th17 induction. This IL-23/γδT17/neutrophil axis is critically involved in the onset of CVB3 pancreatitis and represents a potential treating target for the disease.

12.
Angiology ; : 3319719887655, 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31749367

RESUMO

Diabetes mellitus, and even prediabetes, has been shown to be independently associated with cardiovascular disease. Our study explored whether the combination of glycosylated hemoglobin (HbA1c) and fasting blood glucose (FBG) can better assess the severity of coronary heart disease (CHD) in elective percutaneous coronary intervention (PCI) patients. We consecutively enrolled 1006 prediabetic patients with HbA1c 5.7% to 6.4% who underwent elective PCI. Patients were divided into 2 groups: a normal fasting glucose (NFG) group (FBG <6.1 mmol/L) and an impaired fasting glucose (IFG) group (6.1 ≤FBG<7.0 mmol/L) with defined values. Baseline characteristics and angiography data of the 2 groups were compared. The prevalence of 3-vessel disease (P = .002), the GENSINI (the score is named after a professor) score (P = .002), and the SYNTAX (SYNergy between PCI with TAXUS™ and Cardiac Surgery) score (P = .002) of the IFG group was significantly higher compared to the NFG group. After multiple regression analysis, FBG was found to be independently associated with prevalence of 3-vessel disease (adjusted odds ratio: 1.62; 95% confidence interval: 1.21-2.36; P = .013), the GENSINI score (standardized ß = .138, P = .008), and the SYNTAX score (standardized ß = .145, P = .005). In addition, HbA1 c was independently associated with the prevalence of 3-vessel disease, the GENSINI, score, and the SYNTAX score (P < .05). Both FBG and HbA1c are independently correlated with the severity of CHD in prediabetic patients with HbA1c 5.7% to 6.4%.

13.
J Food Sci ; 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31750942

RESUMO

The human gastrointestinal tract represents one of the most densely populated microbial ecosystems studied to date. Although this microbial consortium has been recognized to have a crucial impact on human health, its precise composition is still subject to intense investigation, as people from different regions have different gut microbiota structures. The Kazakh nomads in Xinjiang, China still retain their nomadic lifestyle and traditional diet. Their specific diet style and ancient genetic background shaped their gut microbiota to contain unique characteristics. In present study, the compositions of the gut microbiota and fermented dairy foods were assessed by high-throughput sequencing of the 16S rRNA gene. Twenty-nine Kazakh nomads were recruited, and 33 traditional fermented dairy foods were collected from five pasturing areas (Buerjin, Zhaosu, Nilka, Tekes, and Fuhai) in northern Xinjiang, China. The correlation of the physical index with the gut microbiota was also analyzed. The unique diet style of Kazakh may be a critical factor in keeping their gut microbiota in a balanced state and help them to remain in good health. PRACTICAL APPLICATION: This research shows that the consumption of spontaneous fermented dairy food plays an important role in increasing gut microbial diversity. Some probiotics in fermented dairy food, such as Bifidobacterium and Lactobacillus, have positive correlation with human body health index such as body mass index and blood glucose. These may provide some theoretical supports to adjuvant therapy of obesity and diabetes through scientific dietary intervention.

14.
J Orthop Surg Res ; 14(1): 382, 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31752900

RESUMO

BACKGROUND: Geriatric hip fracture is a common type of osteoporotic fracture with high mortality and disability; surgical site infection (SSI) can be a devastating complication of this injury. By far, only a few studies identified easily remediable factors to reduce infection rates following hip fracture and less researches have focused on geriatric patients. The objective of this study was to identify potentially modifiable factors associated with SSI following geriatric hip fracture surgery. METHODS: This retrospective, multicenter study involves three level I hospitals. A total of 1240 patients (60 years or older) underwent hip surgery with complete data were recruited between January 2016 and June 2018. Demographics information, medications and additional comorbidities, operation-related variables, and laboratory indexes were extracted and analyzed. Receiver operating characteristic (ROC) analysis was performed to detect the optimum cut-off value for quantitative data. Univariate and multivariate logistic analysis model were performed respectively to identify the independent predictors. RESULTS: Ninety-four (7.58%) patients developed SSI in this study, and 76 (6.13%) had superficial infection, while 18 (1.45%) were diagnosed with deep infection. Results of univariate and multivariate analysis showed age > 79 years (OR, 2.60; p < 0.001), BMI > 26.6 kg/m2 (OR, 2.97; p < 0.001), operating time > 107 min (OR, 2.18; p = 0.001), and ALB < 41.6 g/L (OR, 2.01; p = 0.005) were associated with an increased incidence of SSI; drainage use (OR, 0.57; p = 0.007) could reduce the incidence of wound infection for patients after geriatric hip fracture. CONCLUSION: Accurate modifiable variables, operating time > 107 min, serum albumin < 41.6 g/L, BMI > 26.6 kg/m2, and age > 79 years could be applied to distinguish geriatric patients with high-risk of postoperative surgical site infection.

15.
Analyst ; 144(23): 6866-6870, 2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31670735

RESUMO

Surfactants play important roles in chemical industries and have become well-known environmental pollutants owing to their extensive use in different fields. In this work, we reported a fluorescent probe, namely, BDP-Zn2+ for the discrimination of four kinds of surfactants and the determination of CMC values. BDP-Zn2+ was composed of covalently linked BODIPY, carbazole, N,N-bis(2-pyridylmethyl)ethylenediamine (BPEA) and zinc ions to fabricate a novel push-pull molecular structure. Upon the addition of surfactants, the probe exhibited a turn-on fluorescence response and the emission was enhanced on increasing the surfactant concentrations. This indicated that the fluorescence intensity and the ratios of the emission at 607 nm to that at 514 nm as fingerprints could be used to identify the CMC values of the surfactants. Our current work provides an alternative method to efficiently discriminate different surfactants for the further studies of their physical and chemical functions.

16.
J Agric Food Chem ; 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31746600

RESUMO

We compared phospholipids (PLs), PLs fatty acid (FA) composition and milk fat globules size and structure in human milk (n = 120) from mothers of full-term and preterm infants during lactation (colostrum, transition, 1 mo, 2 mo, and 3 mo) and 8 brands of infant formulae. The absolute quantification of PLs was analyzed using 31P NMR spectroscopy. Sphingomyelin (SM) was the dominant PLs (35.01 ± 3.31%) in human milk, whereas phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were the dominant PLs in infant formulae. The PLs content in preterm milk increased during lactation, whereas that in term milk remained stable. Saturated FAs (mainly 16:0 and 18:0) were the most abundant (>60%) PLs FA in both preterm and term milk and increased throughout lactation. The mean diameter of milk fat globules in infant formulae was much smaller than that found in human milk (200 nm vs 5.63 µm). Significant differences were observed between human milk and infant formulae with regard to PLs, suggesting that more research is needed to mimic the PLs profile in infant formula.

17.
Dalton Trans ; 48(44): 16776-16785, 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31674607

RESUMO

A novel metal-organic framework with the formula [Zn3(DDB)(DPE)]·H2O (1) (H5DDB = 3,5-di(2',4'-dicarboxylphenyl)benzoic acid and DPE = 1,2-di(4-pyridyl)ethylene) has been solvothermally synthesized by employing a rigid carboxylate ligand H5DDB to assemble with Zn(ii) ions in the presence of a flexible bis(pyridyl) linker DPE. The Zn-MOF is a 3D framework with six-nuclear clusters and possesses remarkable water stability and pH stability. Interestingly, complex 1 can sensitively and selectively sense Fe(iii), Cr(iii), Cr(vi), Mn(vii) and the pesticide 2,6-Dich-4-NA with low detection limits in aqueous solution. Moreover, complex 1 also exhibits selectivity for 2,6-Dich-4-NA detection in real samples including carrot, grape and nectarine extracts, and its detection ability is almost unchanged in the presence of the surfactant sodium dodecyl sulfate (SDS). The possible mechanisms of luminescence quenching have been explained by the weak affinity of nitrogen atoms, resonance energy transfer, and photoinduced electron transfer. To our knowledge, this is the first example of a MOF-based multiresponsive fluorescent probe for the simultaneous detection of Fe(iii), Cr(iii/vi), Mn(vii) and the pesticide 2,6-Dich-4-NA in aqueous solution.

18.
Gigascience ; 8(11)2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31682256

RESUMO

BACKGROUND: Papilio bianor Cramer, 1777 (commonly known as the Chinese peacock butterfly) (Insecta, Lepidoptera, Papilionidae) is a widely distributed swallowtail butterfly with a wide number of geographic populations ranging from the southeast of Russia to China, Japan, India, Vietnam, Myanmar, and Thailand. Its wing color consists of both pigmentary colored scales (black, reddish) and structural colored scales (iridescent blue or green dust). A high-quality reference genome of P. bianor is an important foundation for investigating iridescent color evolution, phylogeography, and the evolution of swallowtail butterflies. FINDINGS: We obtained a chromosome-level de novo genome assembly of the highly heterozygous P. bianor using long Pacific Biosciences sequencing reads and high-throughput chromosome conformation capture technology. The final assembly is 421.52 Mb on 30 chromosomes (29 autosomes and 1 Z sex chromosome) with 13.12 Mb scaffold N50. In total, 15,375 protein-coding genes and 233.09 Mb of repetitive sequences were identified. Phylogenetic analyses indicated that P. bianor separated from a common ancestor of swallowtails ∼23.69-36.04 million years ago. Demographic history suggested that the population expansion of this species from the last interglacial period to the last glacial maximum possibly resulted from its decreased natural enemies and its adaptation to climate change during the glacial period. CONCLUSIONS: We present a high-quality chromosome-level reference genome of P. bianor using long-read single-molecule sequencing and Hi-C-based chromatin interaction maps. Our results lay the foundation for exploring the genetic basis of special biological features of P. bianor and also provide a useful data source for comparative genomics and phylogenomics among butterflies and moths.

19.
Hypertens Res ; 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31700166

RESUMO

Atrial fibrillation (AF) is the most common human arrhythmia in clinical practice and may be promoted by atrial inflammation and fibrosis. Ubiquitination is an important posttranslational modification process that is reversed by deubiquitinating enzymes (DUBs). DUBs play critical roles in modulating the degradation, activity, trafficking, and recycling of substrates. However, less research has focused on the role of DUBs in AF. Here, we investigated the effect of ubiquitin C-terminal hydrolase 1 (UCHL1), an important DUB, on the development of AF induced by angiotensin II (Ang II). Male wild-type mice were treated with the UCHL1 inhibitor LDN57444 (LDN) at a dose of 40 µg/kg and infused with Ang II (2000 ng/kg/min) for 3 weeks. Our results showed that Ang II-infused wild-type (WT) mice had higher systolic blood pressure and an increased incidence and duration of AF. Conversely, this effect was attenuated in LDN-treated mice. Moreover, the administration of LDN significantly reduced Ang II-induced left atrial dilation, fibrosis, inflammatory cell infiltration, and reactive oxygen species (ROS) production. Mechanistically, LDN treatment inhibited the activation of multiple signaling pathways (the AKT, ERK1/2, HIF-1α, and TGF-ß/smad2/3 pathways) and the expression of CX43 protein in atrial tissues compared with that in vehicle-treated control mice. Overall, our study identified UCHL1 as a novel regulator that contributes to Ang II-induced AF and suggests that the administration of LDN may represent a potential therapeutic approach for treating hypertensive AF.

20.
Artigo em Inglês | MEDLINE | ID: mdl-31702120

RESUMO

Preterm birth and its complications are the leading cause of neonatal death. The main underlying pathological mechanisms for preterm complications are disruption of the normal maturation processes within the target tissues, interrupted by premature birth. Cord blood, as a new and convenient source of stem cells, may provide new, promising options for preventing preterm complications. This prospective, nonrandomized placebo controlled study aims at investigating the effect of autologous cord blood mononuclear cells (ACBMNC) for preventing preterm associated complications. Preterm infants less than 35 weeks gestational age were assigned to receive ACBMNC (5 × 107 cells/kg) intravenous or normal saline within 8 hours after birth. Preterm complications rate were compared between two groups to demonstrate the effect of ACBMNC infusion in reducing preterm complications. Fifteen preterm infants received ACBMNC infusion, and 16 infants were assigned to control group. There is no significant difference when comparing mortality and preterm complications rate before discharge home. However, ACBMNC infusion demonstrated significant decreases in duration of mechanical ventilation (3.2 days vs 6.41 days, P = .028) and oxygen therapy (5.33 days vs 11.31 days, P = .047). ACBMNC infusion was effective in reducing respiratory support duration in very preterm infants. Due to the limited number of patients enrolled, powered randomized controlled trials are needed to better define its efficacy. Stem Cells Translational Medicine 2019.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA