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1.
Zhonghua Nei Ke Za Zhi ; 58(10): 751-757, 2019 Oct 01.
Artigo em Chinês | MEDLINE | ID: mdl-31594173

RESUMO

Objective: To investigate the characteristics of body composition (BC) in gout patients and its clinical significance. Methods: Consecutive gout patients were recruited between August 2017 and December 2018. Demographic information, clinical characteristics and comorbidities were collected. BC was assessed by bioelectric impedance analysis including body fat percentage (BF%), trunk and limb BF%, appendicular skeletal muscle index. Overfat was defined by BF% ≥25% for male and ≥35% for female. The association between BC and serum uric acid (sUA) was evaluated by multiple linear regression. Results: A total of 362 gout patients were recruited with median age 38 (30, 52) years, 96.1% (348/362) were male. Mean sUA was (551±133) µmol/L. The mean BF% was (25.8±6.4)% with 53.6%(194/362) patients overfat. Male gout patients with overfat showed more affected joints [4(2, 6) vs. 2(2, 5)], higher sUA [(576±126)µmol/L vs. (523±134) µmol/L], higher prevalence of dyslipidemia [70.1%(131/187) vs. 54.0%(87/161)], metabolic syndrome [60.8%(118/187) vs. 28.0%(47/161)], fatty liver [58.2%(113/187) vs. 35.1%(59/161)] and hypertension [44.4%(83/187) vs. 25.5%(41/161)] than male patients with normal fat (all P<0.05). Their BF%, trunk BF% and limb BF% were positively correlated with the numbers of affected joints, sUA, metabolic syndrome, fatty liver, and hypertension, respectively (r=0.154-0.435, all P<0.05). Multivariable linear regression suggested that BF% (ß=4.29, P=0.020) and trunk BF% (ß=9.11, P=0.007), but not limb BF%, were positively correlated with sUA. Conclusion: Overfat is very common in gout patients. The proportion of trunk fat in male patients is positively correlated with sUA. When assessing obesity in gout patients clinically, body composition analysis should be performed simultaneously.


Assuntos
Composição Corporal/fisiologia , Gota/diagnóstico , Obesidade/epidemiologia , Ácido Úrico/sangue , Adulto , Dislipidemias/epidemiologia , Feminino , Gota/sangue , Gota/epidemiologia , Humanos , Hipertensão/sangue , Hipertensão/epidemiologia , Masculino , Pessoa de Meia-Idade , Hepatopatia Gordurosa não Alcoólica/sangue , Hepatopatia Gordurosa não Alcoólica/epidemiologia , Obesidade/sangue , Prevalência
2.
Eur Rev Med Pharmacol Sci ; 23(7): 2856-2862, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31002136

RESUMO

OBJECTIVE: The aim of this study was to determine the role of microRNA-506-3p (miR-506) in papillary thyroid carcinoma (PTC), and to further explore the underlying mechanism. PATIENTS AND METHODS: The expression level of miR-506 in clinical cases was detected by Real Time-fluorescence quantitative Polymerase Chain Reaction (RT-qPCR). Meanwhile, RT-qPCR was performed to determine miR-506 expression in different PTC cell lines. Bioinformatics software was used to predict the possible target genes of miR-506. Dual-Luciferase reporter gene assay together with Western blot (WB) assay were used to verify the prediction results. Finally, cellular functions such as proliferation and metastasis capacities were detected in vitro. RESULTS: RT-qPCR was used to measure the expression level of miR-506 in 80 paired PTC cases. The results showed that the expression level of miR-506 in PTC tissues was significantly decreased. In vitro, miR-506 expression was also markedly suppressed in four PTC cell lines. TPC-1 cells expressed the lowest level of miR-506. Subsequently, the target gene of miR-506 was predicted by TargetScan, miRBase and miRanda. The prediction results indicated that IL17RD was an alternative target gene of miR-506. Furthermore, miR-506 was found to remarkably inhibit the Luciferase activity of wild-type IL17RD. However, it had no effect on mutant-type. Besides, the protein expression level of IL17RD was significantly reduced in miR-506-overexpressing TPC-1 cells. More importantly, the restored expression of IL17RD could alleviate the blocking effects of miR-506 on cell proliferation, migration and invasion. CONCLUSIONS: In this study, we found that miR-506 could inhibit the proliferation and metastasis of PTC cells. Meanwhile, IL17RD might be a downstream target of the biological process. Our findings provided a new therapeutic direction for the treatment of PTC.

3.
J Med Entomol ; 55(6): 1402-1409, 2018 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-30060116

RESUMO

Aldrichina grahami (Aldrich) (Diptera: Calliphoridae) is a forensically important species mainly found in Asia and is one of the first species to colonize a corpse in early spring and late autumn when the ambient temperature is slightly lower. In this study, A. grahami was collected from the Yangtze River Delta region and reared at seven constant temperatures ranging from 16 to 34°C to investigate the indices of development duration, accumulated degree hours, and larval body length. Using these results, we generated an isomorphen diagram, thermal summation model, and isomegalen diagram for A. grahami. Equations of the variation in larval body length with time after hatching and variation in time after hatching with body length were also obtained. A. grahami can complete its life cycle in 16-28°C, while its development was incomplete at 31 and 34°C, where puparia failed to transform into adults and eggs failed to hatch, respectively. The mean (±SD) developmental durations of A. grahami from egg to adult at 16, 19, 22, 25, and 28°C were 685.5 ± 18.8, 540.3 ± 15.9, 454.3 ± 19.4, 388.8 ± 19.0, and 335.5 ± 8.7 h, respectively. The mean (±SE) developmental threshold temperature D0 and the thermal summation constant K were determined as 4.33 ± 0.21°C and 7,985.9 ± 104.5 degree hours, respectively. The results of this study provide fundamental development data for the use of A. grahami in minimum postmortem interval estimations.


Assuntos
Dípteros/crescimento & desenvolvimento , Modelos Biológicos , Animais , Ciências Forenses , Temperatura Ambiente
4.
Nanoscale ; 10(19): 9268-9275, 2018 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-29736512

RESUMO

Acid-stable and efficient hydrogen evolution reaction (HER) catalysts have been widely reported. However, achieving full water splitting catalysis in acidic electrolytes is still an urgent task due to the lack of efficient, abundant and acid-stable oxygen evolution reaction (OER) catalysts. This rationally designed MoSe2 nanosheet/MoO2 nanobelt/carbon nanotube membrane (MoSe2 NS/MoO2 NB/CNT-M) is composed of highly conductive CNTs and hierarchical MoSe2 nanosheets on MoO2 nanobelts, and due to its flexible three-dimensional (3D) electrodes, possesses an open architecture for rapid ion/electron diffusion, maximising the exposure of its active sites. The MoSe2 NS/MoO2 NB/CNT-M achieved remarkable electrocatalytic performance for the HER, providing a geometrical current density of 1 mA cm-2 at an onset potential of -23 mV vs. RHE, a small Tafel slope (69.4 mV dec-1) and excellent long-cycle stability in 0.5 M H2SO4. More importantly, the obtained multifunctional electrodes delivered excellent electrocatalytic activity towards the OER under acidic conditions, with a very small onset potential (≈+1.43 V vs. RHE) and enhanced long-cycle durability. The two-electrode electrolyzer, composed of a MoSe2 NS/MoO2 NB/CNT-M both as an anode and a cathode, required a small voltage of 1.63 V to achieve 10 mA cm-2 and improved electrocatalytic durability, which was better than that of an electrolyzer composed of RuO2//20 wt% Pt/C. This work highlights the potential for active and abundant non-precious catalysts for full water splitting in acidic electrolytes.

6.
7.
Cell Prolif ; 50(5)2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28758259

RESUMO

OBJECTIVES: Investigate the role of the transient receptor potential melastatin 4 (TRPM4) channel in rat dental pulp stem cell (DPSC) proliferation and survival. MATERIALS AND METHODS: Immunofluorescence and FACS analysis were used to detect the stem cell marker CD90. Alizarin Red S and Oil Red O staining were used to identify osteoblast and adipocyte differentiation, respectively. To characterize TRPM4, patch-clamp recordings were obtained from single cells in the whole-cell configuration mode. The significance of TRPM4 for proliferation and survival was examined with 9-phenanthrol, a TRPM4 inhibitor during a 96-hour period of culture. Real-time Ca2+ imaging analysis with Fura-2AM was used to investigate the impact of TRPM4 on intracellular Ca2+ signals. RESULTS: DPSCs were CD90-positive and differentiated into osteoblasts. Patch-clamp recordings revealed currents typical of TRPM4 that were Ca2+ -activated, voltage-dependent and Na+ -conducting. Inhibition of TRPM4 resulted in a significant reduction in the cell population after a 96-hr period of culture and transformed the biphasic pattern of intracellular Ca2+ signalling into sustained oscillations. CONCLUSIONS: Rat DPSCs have stem cell characteristics and functional TRPM4 channels that are required for proliferation and survival. These data suggest that the shape and frequency of intracellular Ca2+ signals may mediate stem cell proliferation and survival.


Assuntos
Adipócitos/citologia , Proliferação de Células , Polpa Dentária/citologia , Osteoblastos/citologia , Células-Tronco/citologia , Canais de Cátion TRPM/metabolismo , Adipócitos/metabolismo , Adipogenia , Animais , Sinalização do Cálcio , Diferenciação Celular , Sobrevivência Celular , Células Cultivadas , Polpa Dentária/metabolismo , Osteoblastos/metabolismo , Osteogênese , Ratos , Células-Tronco/metabolismo , Antígenos Thy-1/análise , Antígenos Thy-1/metabolismo
8.
Zhonghua Bing Li Xue Za Zhi ; 46(4): 240-244, 2017 Apr 08.
Artigo em Chinês | MEDLINE | ID: mdl-28376589

RESUMO

Objective: To study the expression status and clinical significance of PTEN and NDRG1 in colorectal carcinoma. Methods: Tissue samples of 91 colorectal cancers, 30 colorectal adenomas and 21 colorectal normal mucosa tissues were collected. Postoperative specimens were examined by immunohistochemistry for PTEN and NDRG1 expression. The expression of PTEN and NDRG1 was correlated with clinicopathological feature. Results: The expression of PTEN and NDRG1 in the studied cases was detected in 55.0%(50/91) and 76.9%(70/91), respectively. Their expression was significantly different from that of colorectal adenomas and normal colorectal mucosa tissues(P<0.05). Decreased expression of PTEN and over expression of NDRG1 were significantly related to the lymph node metastasis (P<0.05). The expression of PTEN was negatively related to that of NDRG1 in colorectal carcinoma(rs'=-0.251, P=0.016). The patients with negative expression of PTEN showed a lower disease free survival and overall survival(P<0.05). Conclusions: Loss of expression of PTEN protein may be an important molecular marker in predicting the occurrence and PTEN may be useful as a prognostic marker of colorectal carcinoma. NDRG1 plays a role in the development of colorectal carcinoma, although not a prognostic indicator.The ancillary study with combined detection of PTEN and NDRG1 may be useful in difficult cases.


Assuntos
Proteínas de Ciclo Celular/metabolismo , Neoplasias Colorretais/metabolismo , Neoplasias Colorretais/mortalidade , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Proteínas de Neoplasias/metabolismo , PTEN Fosfo-Hidrolase/metabolismo , Adenoma/metabolismo , Adenoma/mortalidade , Intervalo Livre de Doença , Feminino , Humanos , Imuno-Histoquímica , Mucosa Intestinal/metabolismo , Metástase Linfática , Masculino , Prognóstico
9.
Artigo em Chinês | MEDLINE | ID: mdl-28104018

RESUMO

Objective: Using scanning electron microscope to observe the ultrastructure of utricular maculae of mouse. Methods: Ten young (6 to 8 weeks) and ten old (>12 months) mice were executed, and their utricles were harvested and the specimens were processed, using scanning electron microscope to observe the structures of the utricles from the surface of otoconia layer to the roots of hair cell cilia. Results: Under the scanning electron microscope, several ultrastructures were observed, including otoconia layer, unstructured gelatinous extracellular matrix layer, honeycomb-like gelatinous extracellular matrix layer, inter-cilia otoconia and hair cell cilia associated with these structures. When compared with young mouse, the otoconia surface of aged mouse was smoother, the gelatinous extracellular matrix between the adjacent otoconias was thinner. Conclusions: Using SEM, ultrastructures can be clearly observed from surface otoconia layer to the roots of hair cell cilia. By the analysis of the ultrastructure of utricular maculae, it is helpful for investigation of the pathological mechanisms of vestibular diseases, such as otolith diseases.


Assuntos
Máculas Acústicas/ultraestrutura , Fatores Etários , Animais , Cílios , Matriz Extracelular/ultraestrutura , Células Ciliadas Auditivas/ultraestrutura , Doenças do Labirinto/patologia , Camundongos , Microscopia Eletrônica de Varredura , Membrana dos Otólitos/ultraestrutura , Sáculo e Utrículo/ultraestrutura
10.
J Med Entomol ; 54(2): 281-289, 2017 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-28011726

RESUMO

Creophilus maxillosus (L., 1758) is a common and widely distributed beetle species found on corpses, and its development duration is far longer than species belonging to the genus Calliphoridae and Sarcophagidae. Therefore, C. maxillosus can be used as a supplementary indicator to estimate minimum postmortem interval (PMImin), and could greatly extend the range of PMImin when the primary colonizers are no longer associated with the corpse or have emerged from pupae. Better descriptions of C. maxillosus development are needed to apply this species for forensic investigations. In this study, the development of C. maxillosus at seven constant temperatures ranging from 17.5-32.5 °C was studied. Through regression analyses, the simulation equations of larval body length variation with time after hatching were obtained. Isomegalen diagrams of the changes of larval body length over time at specific temperatures, and the isomorphen diagrams on the duration of different developmental milestones at specific temperatures were generated. In addition, thermal summation models of different developmental stages and the overall development process of C. maxillosus were generated through regression analysis, by estimating the development threshold temperatures (D0) and the thermal summation constants (K). These results provide important tools for forensic investigations to generate a long-range of PMImin estimation based on the development of C. maxillosus.


Assuntos
Besouros/crescimento & desenvolvimento , Suínos/parasitologia , Animais , Tamanho Corporal , Cadáver , Feminino , Patologia Legal , Larva/crescimento & desenvolvimento , Masculino , Mudanças Depois da Morte , Pupa/crescimento & desenvolvimento , Temperatura Ambiente
11.
Acta Virol ; 60(3): 234-41, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27640433

RESUMO

To design a vaccine that simultaneously prevents both rotavirus (RV) and poliovirus (PV), a PV type 1 (PV1) chimeric protein using RV VP6 as a vector (VP6F) was constructed, expressed in Escherichia coli expression system and characterized by SDS-PAGE, Western blot, immunofluorescence assay and neutralization test. The results showed that the chimeric protein reacted with anti-VP6F and anti-PV1 antibodies and elicited production of serum antibodies against the chimeric protein in guinea pigs. Antibodies against the chimeric protein neutralized RV Wa and PV1 infection in vitro. The results provided a relevant possibility of developing novel approaches in the rational design of vaccines effective against both RV and PV.


Assuntos
Epitopos/imunologia , Poliovirus/classificação , Proteínas Recombinantes/imunologia , Proteínas Virais/imunologia , Animais , Linhagem Celular , Epitopos/genética , Escherichia coli/metabolismo , Regulação Viral da Expressão Gênica , Cobaias , Modelos Moleculares , Plasmídeos , Conformação Proteica , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Células Vero , Proteínas Virais/genética , Proteínas Virais/metabolismo
12.
Zhonghua Nei Ke Za Zhi ; 55(6): 451-4, 2016 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-27256607

RESUMO

OBJECTIVE: To investigate the diagnostic value of plasma aldosterone-to-active-renin ratio(ARR)in combination with plasma aldosterone concentration(PAC)in the predication of aldosteronoma(APA). METHODS: A total of 85 APA and 155 essential hypertension(EH)patients from January 2012 to December 2014 in Chinese PLA General Hospital were enrolled. The ROC curve was applied to calculate the optimal cut-off points of ARR for APA. RESULTS: (1)The optimal cut-off point of supine ARR was 1 707.4(pmol/L)/(µg·L(-1)·h(-1))[61.64(ng/dl)/(µg·L(-1)·h(-1))] with the sensitivity, specificity and accuracy of 89.41%, 80.65% and 83.75%, respectively. The specificity and accuracy of the diagnostic value for APA increased (89.03% and 87.5% respectively) when supine ARR cut-off point were used in combination with supine PAC over 329.4 pmol/L. (2) The optimal cut-off point of upright ARR was 741.5 (pmol/L)/(µg·L(-1)·h(-1))[26.77(ng/dl)/(µg·L(-1)·h(-1) )]with the sensitivity, specificity and accuracy of 85.88%, 91.61% and 89.58%, respectively. Similarly, the specificity and accuracy greatly improved (94.84% and 91.67%, respectively) when upright ARR were applied together with upright PAC over 323.1 pmol/L. CONCLUSIONS: Both spine and upright ARR can be used in screening for APA. Moreover, the specificity and accuracy could be improved when ARR and PAC were used together both in the supine and upright position.


Assuntos
Aldosterona/sangue , Hiperaldosteronismo/diagnóstico , Hipertensão/sangue , Postura , Renina/sangue , Grupo com Ancestrais do Continente Asiático , China , Hipertensão Essencial , Humanos , Hiperaldosteronismo/sangue , Hiperaldosteronismo/etnologia , Hipertensão/etiologia , Valor Preditivo dos Testes , Curva ROC , Sensibilidade e Especificidade
13.
Genet Mol Res ; 15(2)2016 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-27173216

RESUMO

Results from previous studies on the association between methylenetetrahydrofolate reductase (MTHFR) polymorphisms C677T and A1298C and lung cancer have been conflicting. The aim of this meta-analysis was to clarify the effect of MTHFR polymorphisms on the risk of lung cancer. An electronic search of PubMed, EMBASE, the Cochrane library, and the China Knowledge Resource Integrated Database for papers on C677T and A1298C and susceptibility to lung cancer was performed. The STATA software (Version 13.0) was used for statistical analysis. Statistical heterogeneity, tests of publication bias, and a sensitivity analysis were performed. Twenty-six studies on C677T (12,324 cases and 12,532 controls) and thirteen studies on A1298C (6773 cases and 8207 controls) were included in the meta-analysis. The MTHFR C677T polymorphism showed significant pooled ORs for the homozygote comparison (TT versus CC: OR = 1.518, 95%CI = 1.220-1.890), heterozygote comparison (CT versus CC: OR = 1.053, 95%CI = 0.940-1.179), dominant model (CT + TT versus CC: OR = 1.143, 95%CI = 1.013-1.291), recessive model (TT versus CT + CC: OR = 1.435, 95%CI = 1.190-1.730), and additive model (T versus C: OR = 1.176, 95%CI = 1.066-1.298). In summary, our meta-analysis showed that the MTHFR C677T polymorphism is associated with a significant increase in lung cancer risk in Asian and overall populations, but not in Caucasian populations. However, no significant association between the MTHFR A1298C polymorphism and lung cancer risk was found in either the Caucasian or Asian group with any genetic models.


Assuntos
Neoplasias Pulmonares/genética , Metilenotetra-Hidrofolato Redutase (NADPH2)/genética , Polimorfismo de Nucleotídeo Único , Grupo com Ancestrais do Continente Asiático , Estudos de Casos e Controles , Grupo com Ancestrais do Continente Europeu , Humanos , Neoplasias Pulmonares/etnologia
14.
Eur Rev Med Pharmacol Sci ; 20(7): 1271-82, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27097946

RESUMO

OBJECTIVE: The present study aims to investigate the correlation between Interleukin (IL)-17 gene polymorphism with gastric cancer susceptibility in Han Chinese population. PATIENTS AND METHODS: Between November 2013 and October 2014, 386 patients with gastric cancer who had undergone surgeries at our institution and 374 age- and sex-matched healthy controls were included in this study. Single nucleotide polymorphisms (SNPs) of IL-17 gene (rs2275913, rs3748067, rs4711998 and rs763780) in patients and health controls were studied by using restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) and DNA sequencing technology. The correlation between IL-17 polymorphism and gastric cancer susceptibility was analyzed using logistic regression analysis. RESULTS: For IL17 rs2275913, no significant differences were observed in the frequencies of AA genotype and A allele between patients and controls (c2 = 0.870 p > 0.05; c2 = 0.814 p > 0.05). In IL17 rs3748067, the frequencies of TT genotype and T allele were significantly higher in patients than in controls (c2 = 12.82 p < 0.01; c2 = 12.805 p < 0.01). For IL17A rs4711998, no significant differences were observed in the frequencies of AA genotype and A allele between patients and controls (c2 = 2.636, p > 0.05; c2 = 1.462, p > 0.05). As for ILl7F rs763780, the frequencies of GG genotype and G allele in patients were significantly different from those in controls (c2 = 16.534, p < 0.01; c2 = 16.399, p < 0.01). CONCLUSIONS: Polymorphism of IL-17 rs3748067 and rs763780 is closely associated with gastric cancer development. Polymorphism of L-17 rs2275913 and rs4711998 may be correlated with the risk for gastric cancer.


Assuntos
Interleucina-17/genética , Polimorfismo de Nucleotídeo Único , Neoplasias Gástricas/genética , Adulto , Alelos , Grupo com Ancestrais do Continente Asiático/genética , Estudos de Casos e Controles , Feminino , Predisposição Genética para Doença , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Polimorfismo de Fragmento de Restrição
15.
Eur Rev Med Pharmacol Sci ; 20(2): 225-31, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26875889

RESUMO

OBJECTIVE: To evaluate the efficacy and safety of topical 5-aminolevulinic acid (ALA) mediated photodynamic therapy (PDT) for the treatment of severe acne vulgaris. PATIENTS AND METHODS: A total of 125 patients with Pillsbury III-IV severe facial acne were treated with 3 courses of ALA-PDT with an interval of 2 weeks. ALA gel (3.6%) was applied topically to acne lesions for 1.5 h. The lesions were irradiated by a LED light of 633 nm with a light intensity of 66 mW/cm² and a light dose of 126 J/cm². Patients were evaluated for efficacy and safety at weeks 2, 4, 6, 8, 12 after the initial treatment. RESULTS: The total effective rates were 1.6%, 24.8%, 68.8%, 89.6% and 88.8% at the 2- 4- 6- 8- and 12-week after the initial treatment respectively. The clinical outcomes were the best at 4 weeks after the final treatment. The best effective rate and cure rate were 89.6% and 44% respectively. 26 patients and 16 patients showed apparent exacerbation of acne lesions before the 2nd and 3rd treatment respectively, but all of them showed good or excellent improvement after 3-course treatment. Adverse reactions were mild and transient. CONCLUSIONS: 3.6% ALA-PDT is a simple, safe and effective therapeutic option for the treatment of severe acne vulgaris.


Assuntos
Acne Vulgar/tratamento farmacológico , Ácido Aminolevulínico/administração & dosagem , Fotoquimioterapia , Fármacos Fotossensibilizantes/administração & dosagem , Administração Cutânea , Adolescente , Adulto , Ácido Aminolevulínico/uso terapêutico , Feminino , Humanos , Masculino , Fármacos Fotossensibilizantes/uso terapêutico , Resultado do Tratamento , Adulto Jovem
16.
Zhonghua Yi Xue Za Zhi ; 96(48): 3879-3884, 2016 Dec 27.
Artigo em Chinês | MEDLINE | ID: mdl-28057157

RESUMO

Objective: To analyze the clinical features and outcomes of congenital adrenal hyperplasia (CAH) with adenomatoid adrenal gland. Methods: Nineteen patients clinically confirmed as CAH with adenomatoid adrenal gland, from 2008 to 2015 in Deparment of Endocrinology of Chinese PLA General Hospital, were retrospectively analyzed, and the outcomes of the treatment were followed up. Results: Seventy-six patients were clinically confirmed as CAH, from 2008 to 2015 in PLA hospital. Nineteen of them have accompanied with adenomatoid adrenal gland. Of the 19 confirmed cases, 7 were male, and 12 were female. The mean age was (33.3±14.8) years old. Nineteen confirmed cases were consisted of 10 cases of 21-hydroxylase deficiency (OHD), 6 cases of 17α-OHD, and 3 cases of 11ß-OHD. Of the 19 cases, 5 cases presented with adrenal hyperplasia with left side adenomatoid solid lesion, 2 cases presented with adrenal hyperplasia with right side adenomatoid solid lesion, and 8 cases presented with double side adenomatoid solid lesion. The proportion of adrenal hyperplasia with unilateral cystic or calcified changes was 21.1%. Nine of the 19 patients underwent operation or fine needle biopsy. The pathology showed 7 adenomas (21-OHD/17α-OHD 5/2) and 2 myelolipomas (21-OHD/17α-OHD 1/1). Four patients were admitted into hospital because of the incidentaloma, while others because of the clinical symptoms. The average follow-up time was 31 months. CT images were rechecked among 7 patients. After the treatment, 1 patient's tumor shrunk, 4 cases had no recurrence, 1 case's tumor disappeared, and the other one case had no change. Symtoms of eleven followed-up patients relieved. Conclusions: The image of adrenal of CAH with adenomatoid adrenal gland is various. It may occur in each type of CAH presented as unilateral or bilateral, adenoma or myelolipoma.


Assuntos
Hiperplasia Suprarrenal Congênita/complicações , Recidiva Local de Neoplasia , Adenoma , Adolescente , Glândulas Suprarrenais , Hiperplasia Suprarrenal Congênita/patologia , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Adulto Jovem
17.
Genet Mol Res ; 14(4): 17099-108, 2015 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-26681057

RESUMO

Previous studies have shown that the blast fungus, Magnaporthe oryzae, may experience nitrogen starvation during infection of its plant host (rice,Oryza sativa). Here, we studied the expression of seven genes encoding cysteine-rich proteins with N-terminal signal peptides during nitrogen limitation and throughout the infection process. Some genes were upregulated to a greater extent in weak pathogenic strains than in strong pathogenic strains when they were cultured in complete media, and the expression of some genes was higher in both weak and strong pathogenic strains cultured in 1/10-N and nitrogen starvation media. Furthermore, the expression of these genes was upregulated to different extents in the early stages of M. oryzae infection. These data demonstrate that the genes of interest are highly expressed in weak and strong pathogenic strains cultured under nitrogen limitation and at the early stage of the infection process. This indicates that cysteine-rich secreted proteins in the blast fungus might be involved in establishing disease in the host and that they are sensitive to nitrogen levels. Thus, their role in sensing nitrogen availability within the host is implied, which provides a basis for further functional identification of these genes and their products during plant infection.


Assuntos
Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Interações Hospedeiro-Patógeno , Magnaporthe/genética , Magnaporthe/metabolismo , Nitrogênio/metabolismo , Oryza/microbiologia , Regulação Fúngica da Expressão Gênica , Doenças das Plantas/microbiologia
18.
Clin Exp Dermatol ; 40(4): 438-40, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25683325

RESUMO

Erythema induced by ultraviolet (UV)B light is a common skin reaction. Currently, three techniques, the Chromameter(®) CR-400, the Mexameter(®) MX16 and full-field laser perfusion imaging (FLPI), are widely used for dermatological evaluation of UVB-induced erythema. However, there is little known about the comparative performance of these three techniques. This study was therefore designed to evaluate the effectiveness of the three techniques. Our findings showed that the performance of Chromameter and Mexameter for measurement of UVB-induced erythema was very similar, while FLPI indicated acute erythema at D1 with the greatest fold change. Further studies of UVB dose-dependence need to be carried out.


Assuntos
Colorimetria/instrumentação , Dermoscopia/instrumentação , Eritema/diagnóstico , Imagem de Perfusão/métodos , Pele/efeitos da radiação , Raios Ultravioleta/efeitos adversos , Doença Aguda , Adulto , Eritema/etiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Sensibilidade e Especificidade , Adulto Jovem
19.
Neuroscience ; 289: 443-51, 2015 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-25617651

RESUMO

To probe the mechanism underlying the auditory behavior-related response patterns of inferior collicular neurons to constant frequency-frequency modulation (CF-FM) stimulus in Hipposideros pratti, we studied the role of post-spike hyperpolarization (PSH) in the formation of response patterns. Neurons obtained by in vivo extracellular (N=145) and intracellular (N=171) recordings could be consistently classified into single-on (SO) and double-on (DO) neurons. Using intracellular recording, we found that both SO and DO neurons have a PSH with different durations. Statistical analysis showed that most SO neurons had a longer PSH duration than DO neurons (p<0.01). These data suggested that the PSH directly participated in the formation of SO and DO neurons, and the PSH elicited by the CF component was the main synaptic mechanism underlying the SO and DO response patterns. The possible biological significance of these findings relevant to bat echolocation is discussed.


Assuntos
Potenciais de Ação/fisiologia , Percepção Auditiva/fisiologia , Quirópteros/fisiologia , Colículos Inferiores/fisiologia , Neurônios/fisiologia , Estimulação Acústica , Animais , Feminino , Masculino , Microeletrodos
20.
Braz. j. med. biol. res ; 48(1): 34-38, 01/2015. graf
Artigo em Inglês | LILACS-Express | ID: lil-730430

RESUMO

Although radical nephrectomy alone is widely accepted as the standard of care in localized treatment for renal cell carcinoma (RCC), it is not sufficient for the treatment of metastatic RCC (mRCC), which invariably leads to an unfavorable outcome despite the use of multiple therapies. Currently, sequential targeted agents are recommended for the management of mRCC, but the optimal drug sequence is still debated. This case was a 57-year-old man with clear-cell mRCC who received multiple therapies following his first operation in 2003 and has survived for over 10 years with a satisfactory quality of life. The treatments given included several surgeries, immunotherapy, and sequentially administered sorafenib, sunitinib, and everolimus regimens. In the course of mRCC treatment, well-planned surgeries, effective sequential targeted therapies and close follow-up are all of great importance for optimal management and a satisfactory outcome.

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