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1.
Wei Sheng Yan Jiu ; 50(5): 788-798, 2021 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-34749873

RESUMO

OBJECTIVE: To establish a method for the determination of iodine species in human serum by inductively coupled plasma mass spectrometry(ICP-MS). METHODS: Total iodine was determined by ICP-MS in helium mode after dilution of serum with tetramethylammonium hydroxide solution. The serum was added to methanol solution to precipitate the protein, and the supernatant was separated by liquid chromatography(LC) followed by ICP-MS for the determination of inorganic iodine. RESULTS: The limit of detection(LOD) of serum I~- by ICP-MS was 0.17 µg/L, the limit of quantification(LOQ) was 0.57 µg/L, and the linear correlation coefficient R~2=0.9998; the LOD of serum IO_3~- was 0.16 µg/L, the LOQ was 0.55 µg/L, and the linear correlation coefficient R~2=0.9998.The I~- recoveries were 96.2%-104.5% for the total serum iodine assay and 93.7%-98.6% for the inorganic iodine assay. Analysis of the iodine species of the actual serum samples showed that the I~- content was 2.6-12.2 µg/L, the organic iodine content was 45.3-66.0 µg/L, and the serum samples were essentially free of IO_3~-. CONCLUSION: A convenient, efficient and accurate method for the determination of serum iodine species was established using high performance liquid chromatography tandem with inductively coupled plasma mass spectrometry.


Assuntos
Iodo , Cromatografia Líquida de Alta Pressão , Cromatografia Líquida , Humanos , Iodetos , Espectrometria de Massas
2.
China CDC Wkly ; 3(9): 189-192, 2021 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-34595041

RESUMO

Summary: What is already known on this topic? Vitamin A deficiency (VAD) in children is still a global public health problem, which needs continuous monitoring and timely intervention.What is added by this report? Using surveillance data from China Nutrition and Health Surveillance of Children and Lactating Mothers in 2016-2017, the prevalence of VAD and marginal deficiency was 0.96% and 14.71%, respectively. The vitamin A nutritional status of children and adolescents from urban areas and those aged 12-17 years were better than those from rural areas and aged 6-11 years.What are the implications for public health practice? Marginal VAD was a major form of VAD in Chinese children. The monitoring of vitamin A status in key populations should be continuously strengthened, and the public should be encouraged to consume foods rich in vitamin A or vitamin A supplements.

4.
Nutrients ; 13(9)2021 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-34578963

RESUMO

OBJECTIVES: To analyze the serum zinc nutrition status of the Chinese elderly, and to assess the risk factor for zinc deficiency. METHODS: 3727 elderly people over 60 years old were randomly selected from 302 monitoring points in 31 provinces of China Adult Chronic Disease and Nutrition Surveillance (CACDNS) 2015. Blood samples were selected from the biological sample bank of CACDNS 2015 and the basic information were collected by questionnaires. The criteria of serum zinc deficiency recommended by the International Zinc Nutrition Consulting Group (IZiNCG) were adopted, and the related factors were also analyzed. RESULTS: The median serum zinc concentration of Chinese elderly was 99.2 (84.3~118.7) µg/dL. The overall adjusted prevalence of zinc deficiency was 8.68%, with a 95% confidence interval (CI) of 7.74% and 9.61%. Significant differences were found in different sex, age groups, Body Mass Index (BMI), residence region, race and education level in terms of serum zinc status in the elderly (p < 0.05). Participants who are males, aged above 70y, with BMIs of less than 18.5 kg/m2, living in rural areas, minority, and with the lowest income had a higher prevalence of zinc deficiency in the subgroups under different classifications. The risk of zinc deficiency in the elderly over 70y was 1.44 higher than those aged 60-69y (OR = 1.44, 95%CI 1.14-1.82), and the minority elderly has a 1.39 higher risk than Han nationality (OR = 1.39, 95%CI 1.01-1.91), while overweight/obesity and female elderly were at lower risk (OR < 1, p < 0.05). CONCLUSIONS: The overall prevalence of zinc deficiency in the elderly was highest in all adults in the CACDNS. It is recommended that the male elderly, over 70 years, and the minority elderly should pay more attention to the zinc nutrition status of their own.


Assuntos
Doença Crônica/epidemiologia , Estado Nutricional , Zinco/deficiência , Idoso , Idoso de 80 Anos ou mais , China/epidemiologia , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fatores de Risco , População Rural , Fatores Socioeconômicos , Inquéritos e Questionários , Zinco/sangue
5.
Nutrients ; 13(9)2021 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-34579161

RESUMO

BACKGROUND: Magnesium and calcium play a variety of biological roles in body functions. Reference values of these elements have not yet been systematically determined in China, especially in childbearing women. We proposed to establish the reference range of Mg, Ca, and Ca/Mg ratio in plasma and whole blood for 18-44 years healthy childbearing women in China. METHOD: A total of 1921 women of childbearing age (18-44 years) were randomly selected from the 2015 China National Nutrition and Health Survey by taking into account the regional types and monitoring points. Among them, 182 healthy women were screened out with a series strict inclusion criteria to study the reference ranges of elements. Fundamental indicators (weight, height, waist, blood pressure, total cholesterol, triglyceride, high-density lipoprotein cholesterol, low-density lipoprotein, fast glucose, HbA1c, blood pressure, uric acid) and elements concentrations in plasma and whole blood were collected. The 2.5th to 97.5th was used to represent the reference range of Mg, Ca, and Ca/Mg ratio. RESULTS: The reference range of Mg, Ca, and Ca/Mg ratio in plasma were 0.75-1.13 mmol/L, 2.27-3.43 mmol/L, and 2.41-3.44, respectively. Additionally, the reference range of Mg, Ca, and Ca/Mg ratio in whole blood were 1.28-1.83 mmol/L, 1.39-2.26 mmol/L, and 0.90-1.66, respectively. According to the established reference range, the prevalence of magnesium deficiency was 4.79% in 1921 childbearing women, 21.05% in type 2 diabetes, and 5.63% in prediabetes. CONCLUSION: The reference values of Mg, Ca and Ca/Mg proportion in plasma and entire blood of healthy childbearing women can be utilized as a pointer to assess the status of component lack and over-burden. The lower limit of plasma Mg is in good agreement with the recommended criteria for the determination of hypomagnesemia.


Assuntos
Cálcio/sangue , Magnésio/sangue , Adolescente , Adulto , Fatores Etários , Glicemia/análise , Pressão Sanguínea , China , Feminino , Humanos , Lipídeos/sangue , Inquéritos Nutricionais , Valores de Referência , Adulto Jovem
6.
Nutrients ; 13(7)2021 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-34209755

RESUMO

Optimal vitamin D (vitD) status is beneficial for both pregnant women and their newborns. The aim of this study was to evaluate the vitamin D status of Chinese pregnant women in the latest China Nutrition and Health Surveillance (CNHS) 2015-2017, analyze the risk factors of vitamin D deficiency (VDD), and compare them with those in CNHS 2010-2012. Serum 25 hydroxyvitamin D (25(OH)D) was measured by ELISA method. City type, district, latitude, location, age, vitamin D supplements intake, education, marital status, annual family income, etc., were recorded. The median 25(OH)D concentration was 13.02 (10.17-17.01) ng/mL in 2015-2017, and 15.48 (11.89-20.09) ng/mL in 2010-2012. The vitamin D sufficient rate was only 12.57% in 2015-2017, comparing to 25.17% in 2010-2012. The risk factors of vitamin D inadequacy (25(OH)D < 20 ng/mL) in 2015-2017 were not exactly consistent with that in 2010-2012. The risk factors included season of spring (p < 0.0001) and winter (p < 0.001), subtropical (p < 0.001), median (p < 0.0001) and warm temperate zones (p < 0.0001), the western (p = 0.027) and the central areas (p = 0.041), while vitD supplements intake (p = 0.021) was a protective factor in pregnant women. In conclusion, vitD inadequacy is very common among Chinese pregnant women. We encourage pregnant women to take more effective sunlight and proper vitD supplements, especially for those from the subtropical, warm and medium temperate zones, the western and the central, and in the seasons of spring and winter.


Assuntos
Inquéritos Nutricionais/tendências , Vigilância da População , Complicações na Gravidez/epidemiologia , Deficiência de Vitamina D/epidemiologia , Vitamina D/análogos & derivados , Adulto , China , Estudos Transversais , Suplementos Nutricionais/estatística & dados numéricos , Feminino , Humanos , Estado Nutricional , Gravidez , Complicações na Gravidez/sangue , Estações do Ano , Vitamina D/sangue , Vitamina D/uso terapêutico
7.
Nutr Metab (Lond) ; 18(1): 67, 2021 Jun 27.
Artigo em Inglês | MEDLINE | ID: mdl-34176509

RESUMO

BACKGROUND: Metabolic syndrome (MetS) is significantly associated with the risk of cardiovascular disease and its prevalence is showing a trend of getting younger. Previous studies on the relationship between elements and MetS were mostly reported in adults with single element analysis, while reports in children with combined effects of multiple elements were very limited. The aim of this study is to investigate the association between whole blood Cu, Mg and Zn in both single and combined effects and MetS components in rural Chinese children aged 6-12 years based on the data from 2010-2012 China National Nutrition and Health Survey. METHODS: A total of 911 children (51.2% male, 48.7% female) aged 6-12 years were included. Basic characteristics and MetS component parameters were collected and determined by trained stuffs. Elements were detected by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Multivariate logistic regression analysis was performed to examine the independent relationship between elements and MetS components. RESULTS: In single metal analysis, copper was positively associated with elevated waist (OR = 2.00, 1.18-3.28) and all of the metals were associated with elevated TG. And the comprehensive analysis of multiple elements were mostly consistent with the results of single element analysis (low Cu + high Zn with elevated TG (OR = 2.21, 1.18-4.13), high Cu + low Mg with elevated TG (OR = 0.40, 0.16-0.95), high Cu + high Mg with elevated waist (OR = 2.03, 1.26-3.27)), except the combination of Zn and Mg (high Zn + low Mg with reduced HDL-C (OR = 0.47, 0.28-0.77)). CONCLUSIONS: Our study suggested Cu, Zn and Mg in children are indeed associated with metabolic syndrome components, whether in single element or multi-element combined analysis. The results will be confirmed through additional cohort research.

8.
Wei Sheng Yan Jiu ; 50(2): 175-180, 2021 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-33985620

RESUMO

OBJECTIVE: To estimate the distribution of serum zinc levels, zinc deficiency status and possible influencing factors in 18-60 year-old adults in China. METHODS: Based on the data of China Adult Chronic Disease and Nutrition Surveillance, 3903 blood samples were selected by stratified random sampling. The distribution of serum zinc in adults with different gender, age, regional type and regional distribution were analyzed by inductively coupled plasma mass spectrometry and body mass index(BMI), exercise, smoking, drinking, vitamin A level and other factors on serum zinc concentration and zinc deficiency rate. Multivariate logistic regression model was used to analyze the correlation between zinc deficiency and the influencing factors. RESULTS: In 2015, the median of serum zinc in Chinese adults aged 18-60 years was 103. 44(95%CI 64. 84-186. 12) µg/dL, and the overall zinc deficiency rate was 6. 04%. Serum zinc concentration had statistically significant difference(P<0. 05) in different genders, regional types, regional distribution, vitamin A status, smoking status, drinking status. In the comparison of zinc deficiency rates, ethnic minorities(10. 67 %) was higher than Han(5. 44%), rural area(7. 35%) was higher than urban area(4. 90%), vitamin A deficiency group(9. 12%) was higher than vitamin A normal group(5. 90%), non-exercise group(6. 29%) was higher than sports group(5. 09%). Among different regional distributions, the western region had the highest zinc deficiency rate(7. 33%), and among different BMI groups overweight group had the lowest rate(4. 81%). These differences were statistically significant(P<0. 05). In the analysis of multivariate logistic regression, the risk of zinc deficiency in the vitamin A deficiency group was 1. 89 higher than that of the normal group(OR=1. 89, 95%CI 1. 13-3. 18); and the overweight group had a lower risk of serum zinc deficiency than the normal group(OR=0. 71, 95%CI 0. 52-0. 96). CONCLUSION: The prevalence of serum zinc deficiency among adults aged 18-60 in China is relatively low. Vitamin A deficiency is a risk factor for zinc deficiency and overweight is a protective factor for zinc deficiency.


Assuntos
Deficiência de Vitamina A , Zinco , Adolescente , Adulto , China/epidemiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estado Nutricional , Prevalência , Vitamina A , Deficiência de Vitamina A/epidemiologia , Adulto Jovem
9.
Wei Sheng Yan Jiu ; 50(2): 181-185, 2021 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-33985621

RESUMO

OBJECTIVE: To evaluate the nutrition status of vitamin A in Chinese rural pregnant women from the Chinese National Nutrition and Health Survey 2015(CNNHS 2015). METHODS: A total of 3273 Chinese rural pregnant women were recruited in this study. The concentrations of serum retinol were determined using high performance liquid chromatography. The C-reactive protein and α-acid glycoprotein were determined by immunoturbidimetric method. The distribution of serum retinol status in pregnant women was further described for different regions, age groups, nationalities, body mass index(BMI), education levels, gestational weeks, family income per capita and inflammation levels. The prevalence of vitamin A deficiency and the percentage of marginal vitamin A deficiency in pregnant women were compared in different subgroups. RESULTS: The median concentration of serum retinol(M(P25, P75)) was 0. 47(0. 36, 0. 61) mg/L. There were significant differences among different regions, age groups, education levels, gestational age and per capita household income(P<0. 01). The prevalence of vitamin A deficiency was 1. 2% for deficiency and 10. 5% for marginal deficiency, respectively. Significant differences were observed in age groups, gestational weeks and inflammation levels(P<0. 01). In addition, the prevalence of vitamin A deficiency was relatively serious, 2. 1% for deficiency and 14. 2% for marginal deficiency in the third trimester, 2. 0% for deficiency and 12. 2% for marginal in the 30-44 years. CONCLUSION: It should be pay more attention on the vitamin A nutrition status in the third trimester and the 30-44 years old pregnant women, although the vitamin A status had been greatly improved in Chinese rural pregnant women.


Assuntos
Deficiência de Vitamina A , Vitamina A , Adulto , China/epidemiologia , Estudos Transversais , Feminino , Humanos , Estado Nutricional , Gravidez , Gestantes , Prevalência , Deficiência de Vitamina A/epidemiologia
10.
Wei Sheng Yan Jiu ; 50(2): 186-191, 2021 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-33985622

RESUMO

OBJECTIVE: To analyze the level of vitamin A and its influencing factors of Chinese rural elderly. METHODS: The serum samples of the rural elderly were selected by random sampling method from the biological sample bank of Chinese Adults Nutrition and Chronic Diseases Surveillance in 2015(CANCDS2015). The basic information of the subjects was collected by questionnaire survey. High performance liquid chromatography(HPLC) method was used to determine the vitamin A nutritional status of the population according to the standard recommended by WHO. Multiple logistic regression analysis was explored the relationship between vitamin A status with the potential relevant factors. RESULTS: A total of 3134 elderly from Chinese rural were included in this study. The serum vitamin A concentration(M(P25, P75))was 1. 92(1. 50, 2. 45)µmoL/L. The prevalence of vitamin A deficiency was 0. 99%(95%CI 0. 64%-1. 34%), and the prevalence of vitamin A marginal deficiency was 4. 38%(95%CI 3. 65%-5. 10%). The prevalence of vitamin A marginal deficiency was significantly higher in elderly lived in western region than that lived in eastern and central regions of China. The serum vitamin A adequacy rate of rural elderly who were 80 years old and above or thin was relatively low(P<0. 05). Married elderly had significant lower vitamin A deficiency rate. The result from multivariate logistic regression model analysis showed that gender, age and body mass index had significant effects on vitamin A nutritional status of Chinese urban elderly(P<0. 05). CONCLUSION: The nutritional status of vitamin A in rural elderly in China was better, and the deficiency rate was lower.


Assuntos
Deficiência de Vitamina A , Vitamina A , Adulto , Idoso , Idoso de 80 Anos ou mais , China/epidemiologia , Humanos , Estado Nutricional , Prevalência , População Rural , Deficiência de Vitamina A/epidemiologia
11.
Wei Sheng Yan Jiu ; 50(2): 192-209, 2021 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-33985623

RESUMO

OBJECTIVE: To investigate the association of rs7041 polymorphism of GC gene that encodes the vitamin D-binding protein with serum vitamin D status in Chinese women of childbearing age. METHODS: A total of 1812 plasma samples of women childbearing aged 18-44 years old were selected by stratified random sampling technology from the established biological samples bank of Chinese Chronic Diseases and Nutrition Survey(CCDNS, 2015-2018). The serum 25(OH)D status was detected by enzyme-linked immunosorbent assay. The genotypes of rs7041 in the GC gene were analyzed by improved multiple ligase detection reaction method. RESULTS: A total of 1812 childbearing women aged 18-49 years were included in this study. The frequency of rs7041 genotypes in the study were distributed according to the Hardy-Weinberg equilibrium, indicating sufficient representativeness of our sample. The median serum 25(OH)D status was 16. 69(12. 04, 21. 69)ng/mL. The higher 25(OH)D levels was detected in the overall sample, southern women or women with normal vitamin D status with the CC genotype than the AA genotype(P<0. 05). Before and after correction, the risk of vitamin D insufficiency in the women carrying the CC genotype was decreased significantly compared with the women carrying the AA genotype(OR=0. 571, 95%CI 0. 373-0. 873). And the CC genotype of rs7041 was associated with a significant decrease in risk of 25(OH)D deficiency(in the subgroup of southern childbearing women, OR=0. 284, 95%CI 0. 144-0. 560 and in the subgroup of northern childbearing women, OR=0. 109, 95%CI 0. 015-0. 798). CONCLUSION: The GC rs7041 with A/C polymorphism are significantly correlated with 25(OH)D status in Chinese childbearing women, mutant CC genotype is a protective factor for vitamin D non-normal status risks.


Assuntos
Polimorfismo de Nucleotídeo Único , Vitamina D , Adolescente , Adulto , Grupo com Ancestrais do Continente Asiático/genética , China , Feminino , Genótipo , Humanos , Pessoa de Meia-Idade , Proteína de Ligação a Vitamina D/genética , Adulto Jovem
12.
Nutrients ; 13(5)2021 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-33921931

RESUMO

We evaluated the iodine nutritional status and related factors among school-age children based on the 2016 National Nutrition and Health Surveillance of Children and Lactating Women; 3808 children from Hebei, Guangxi, and Zhejiang province were included in the study. Urinary iodine concentration (UIC), thyroid-stimulating hormone (TSH), body mass index (BMI), vitamin A (VA), and vitamin D (VD) were measured. The abnormal rate of UIC and TSH were assessed. Relationships between UIC/TSH and the possible factors were analyzed. The overall median UIC was 185.14 µg/L, and the median UIC of children aged 8-10 was 164.60 µg/L. Prevalence of iodine deficiency and excess was 13.84% and 14.36%, respectively, and 12.87% of children showed TSH excess. UIC, as well as the abnormal rates of iodine deficiency (ID) and TSH, were significantly different among the three provinces. The median UICs and excess rates increased with age, reaching 211.45 µg/L and 21.35% at age of 14~, while TSH showed the opposite trend. Overweight children tended to have lower UIC and higher TSH. Higher UIC and TSH were found in VA sufficient group (p < 0.01). Further, the VD deficient group had a higher TSH compared to the sufficient group (p < 0.01). Moreover, UI and TSH distribution was obviously different among different vitamin A/D status (p < 0.05). Although the median UIC of school-age children was optimal, there were pockets of inadequate and excessive UI in the three provinces. Compared to the national IDD monitoring results in 2014, the iodine nutritional status of children was greatly improved. Considerations of region, age, BMI, VA, or VD are needed in the future iodine evaluation and surveillance.


Assuntos
Grupo com Ancestrais do Continente Asiático , Iodo/metabolismo , Instituições Acadêmicas , Criança , China , Estudos Transversais , Feminino , Humanos , Iodo/urina , Masculino , Sobrepeso/sangue , Sobrepeso/epidemiologia , Sobrepeso/urina , Magreza/sangue , Magreza/epidemiologia , Magreza/urina , Tireotropina/sangue , Vitamina A/sangue , Vitamina D/sangue
13.
Nutrients ; 13(4)2021 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-33923902

RESUMO

Objective: The purpose of this study was to evaluate serum zinc status of pregnant women in the China Adult Chronic Disease and Nutrition Surveillance (CACDNS) in 2015-2016. Methods: A total of 7147 apparently healthy pregnant women were randomly selected in 302 national monitoring sites. Information on age, race, residence region, education, pregnancy, and family income per annum was collected, and the concentration of serum zinc was determined. The evaluation of serum zinc status was further performed according to the recommendations by the International Zinc Nutrition Consultative Group (IZiNCG). Results: The median concentration of serum zinc was 858.9 µg/L with an interquartile range (IQR) of 712.9 µg/L and 1048.9 µg/L, while the overall prevalence of zinc deficiency was 3.5% with a 95% confidence interval (CI) of 3.0% and 3.9%. Serum zinc status of pregnant women changed greatly in the different categories, particular in pregnancy and family income per annum (p < 0.05), but no significant difference was observed in the prevalence of zinc deficiency (p > 0.05). Conclusions: The lower prevalence of zinc deficiency generally indicated a better zinc status for pregnant women in the CACDNS in 2015-2016. However, a well-designed evaluation system of zinc status for pregnant women should be continually optimized and improved by inducing more parameters such as biochemical, dietary, or functional indicators.


Assuntos
Doença Crônica/epidemiologia , Inquéritos Nutricionais , Zinco/sangue , Adolescente , Adulto , China , Feminino , Humanos , Modelos Logísticos , Análise Multivariada , Gravidez , Adulto Jovem , Zinco/deficiência
14.
Nutrients ; 13(3)2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-33804217

RESUMO

Background: Copper and zinc are both essential elements in humans, that play various biological roles in body functions. Population-based reference values have not yet been established in China especially in childbearing women. The aim of this study is to establish a reference value of Zn, Cu and Cu/Zn ratios in childbearing women aged 18-44 from a representative population in China. Method: A total of 191 healthy childbearing women aged 18-44 years old were enrolled from the China Adult Chronic Disease and Nutrition Surveillance (2015) in this study with a series strict inclusion criteria. Basic biological indicators (weight, height, waist, blood pressure, high-density lipoprotein cholesterol, low-density lipoprotein, total cholesterol, triglyceride, fast glycose, HbA1c, blood pressure, uric acid) and elements levels in plasma and whole blood were collected. The 2.5th to 97.5th was used to represent the reference range of Cu, Zn and Cu/Zn ratio. Results: The reference range of Zn, Cu and Cu/Zn ratio in plasma were 70.46-177.53 µg/dL, 74.30-170.68 µg/dL and 0.54-1.68, respectively. The reference range of Zn, Cu and Cu/Zn ratios in whole blood were 402.49-738.05, 74.63-124.52 and 0.13-0.25 µg/dL, respectively. Conclusion: The reference range of Zn, Cu and Cu/Zn ratios in plasma and whole blood of healthy Chinese childbearing women could be used as an indicator to evaluate the status of element deficiency and overload.


Assuntos
Cobre/sangue , Zinco/sangue , Adolescente , Adulto , China , Estudos Transversais , Feminino , Humanos , Valores de Referência , Adulto Jovem
15.
Wei Sheng Yan Jiu ; 49(5): 699-704, 2020 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-33070813

RESUMO

OBJECTIVE: To study the relationship between 25-hydroxyvitamin D [25(OH)D] concentration and intact parathyroid hormone(iPTH) concentration in the Chinese non-clinical population aged 18-44 y, and to explore the threshold value of determining the appropriate nutritional status of vitamin D. METHODS: A total of 650 plasma samples of adults aged 18-44 years old were selected from the established biological samples' bank of Chinese Chronic Diseases and Nutrition Survey(CCDNS, 2015-2018). Basic information(including age, gender, region type, season, etc. ) was collected by questionnaires, and the waist circumference, height and weight of the subjects were determined unified. North and South area was divided by Qinling Mountains and Huaihe River. The serum 25(OH)D concentration was determined by high performance liquid chromatography tandem mass spectrometer, and the iPTH was determined by electronic chemiluminescence method. The relationship of 25(OH)D and iPTH were analyzed by partial correlation analysis, and the threshold of 25(OH)D was analyzed by locally weighted scatter plot smoothing method after adjusting the iPTH by age, season of blood draw, BMI, waist, sex and latitude. RESULTS: A total of 623 serum samples(293 male, 330 female) of 25(OH)D concentration and iPTH concentration were analyzed after excluding samples with poor blood sample quality. Significant higher 25(OH)D concentration was found in male than female(P<0. 0001). And the samples from the southern China had higher 25(OH)D concentration than those from the northern China(P<0. 0001). Samples from autumn had higher 25(OH)D concentration than those from spring and winter(P<0. 0001). The 25(OH)D concentration of samples from cities was lower than that of rural areas(P=0. 018). The inversely relationship between 25(OH)D concentration and iPTH concentration was observed when the 25(OH)D was below 17. 6 ng/mL for both sexes. The threshold of 25(OH)D for male was 16. 2 ng/mL and it was 25. 6 ng/mL for female. CONCLUSION: The threshold and the relationship between 25(OH)D and iPTH differs by sex among Chinese adults aged 18-44 y. Further study is needed to evaluate the sex-specific ranges of optimal vitamin D.


Assuntos
Deficiência de Vitamina D , Adolescente , Adulto , Grupo com Ancestrais do Continente Asiático , Calcifediol , China/epidemiologia , Feminino , Humanos , Masculino , Hormônio Paratireóideo , Deficiência de Vitamina D/epidemiologia , Adulto Jovem
16.
J Cancer Educ ; 2020 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-33068265

RESUMO

Patient knowledge of risk factors, signs and symptoms associated with oral cancers is crucial for increasing the likelihood of patient presentation for opportunistic screening and reducing delay in patient appraisal for early detection. This study aimed to assess the knowledge of oral cancer and to ascertain socio-demographic factors that influence knowledge amongst adult dental patients attending public clinics in Brisbane, Australia. A convenience sample of 213 adult dental patients who attended the Herston and Stafford public health clinics in Brisbane, Australia, between July and August 2019 participated in the self-administered questionnaire. Multivariate analyses were performed to identify predictors for oral cancer knowledge. Patients were well informed of smoking as a risk factor (n = 135; 84.4%), whereas only 53.8% (n = 82) of participants agreed that heavy alcohol consumption was a risk factor. A larger proportion of participants identified difficulty of moving the tongue (n = 79; 49.4%) and pain on swallowing (n = 72; 45.0%) compared to the proportion who identified fixed red patches (n = 61; 38.1%) and fixed white patches (n = 57; 35.6%) as a sign or symptom. Education level and gender were significant knowledge predictors for alcohol (p = 0.01), old age (p = 0.008) and family history (p = 0.004) as a risk factors for oral cancer. Those with a family history of cancer were more likely to identify a red patch (p = 0.02), bleeding gums (p = 0.001) and altered sensation (p = 0.023) as a sign or symptom of oral cancer. Overall, patient knowledge was greater for risk factors than for signs and symptoms for oral cancer. Symptoms associated with later stages of cancer were recognised by a greater proportion of patients compared to early stages of oral cancer. These results indicate the need for targeted public health initiatives to improve patient knowledge.

17.
Artigo em Inglês | MEDLINE | ID: mdl-32859094

RESUMO

OBJECTIVES: This paper describes the development of a physical activity questionnaire (PAQ) designed for Chinese adolescents and their mothers in urban and rural settings, and reports on results of the PAQ, pedometry, and hand grip dynamometry from the Chinese Children and Families Cohort Study pilot investigation (CFCS). METHODS: As part of a pilot investigation to evaluate the feasibility to follow-up and obtain detailed nutrition, dietary, physical activity, and ultraviolet radiation (UVR) data from CFCS participants, data were collected in 2013 for 93 adolescent/mother pairs from a rural (n = 41) and an urban site (n = 52) in two provinces. Respondents were asked to wear a pedometer for seven days (Omron HJ-151), use a Takei Digital Grip Strength Dynamometer on (each hand; three trials; two separate days), and complete a 39 item, eight domain PAQ covering the past year. Self-reported physical activity (PA) was linked to metabolic equivalent of task (MET) scores in kcal/kg/hr and used to calculate METs for different domains of PA and intensity categories. RESULTS: Compliance was high (95%) in this measurement protocol administered by health staff during a series of data collection efforts at home and local clinics or health centers. Step counts were highly variable, averaging between 5000 and 10000 per day with somewhat higher step counts in rural adolescent boys. Maximum grip strength (Kgs) was greater in children (Mean = 36.5, SE = 0.8) than mothers (Mean = 28.8, SE = 0.8) and similar in the urban (Mean = 29.6, SE = 0.6) compared to the rural (Mean = 29.6, SE = 0.5) communities overall. Grip strength, step counts, and measures of time spent in different activities or activity intensities were uncorrelated. CONCLUSION: Device and question-based measurement of PA and strength were readily accepted in these Chinese urban and rural populations. The PAQ on physical activity in the past year produced some plausible population averages, but individual responses suggested recall challenges. If data about specific activities are required, future studies should explore use of standardized survey questions concerning such fewer specific activities or instruments examining shorter time periods such as one, three, or seven day recalls.


Assuntos
Exercício Físico , Força da Mão/fisiologia , Adolescente , Grupo com Ancestrais do Continente Asiático , Criança , China , Estudos de Coortes , Exercício Físico/fisiologia , Estudos de Viabilidade , Feminino , Humanos , Masculino , Mães , Projetos Piloto , População Rural , Fatores Socioeconômicos , Raios Ultravioleta , População Urbana
18.
Br J Nutr ; 124(11): 1156-1165, 2020 12 14.
Artigo em Inglês | MEDLINE | ID: mdl-32624007

RESUMO

Data on average iodine requirements for the Chinese population are limited following implementation of long-term universal salt iodisation. We explored the minimum iodine requirements of young adults in China using a balance experiment and the 'iodine overflow' hypothesis proposed by our team. Sixty healthy young adults were enrolled to consume a sequential experimental diet containing low, medium and high levels of iodine (about 20, 40 and 60 µg/d, respectively). Each dose was consumed for 4 d, and daily iodine intake, excretion and retention were assessed. All participants were in negative iodine balance throughout the study. Iodine intake, excretion and retention differed among the three iodine levels (P < 0·01 for all groups). The zero-iodine balance derived from a random effect model indicated a mean iodine intake of 102 µg/d, but poor correlation coefficients between observed and predicted iodine excretion (r 0·538 for µg/d data) and retention (r 0·304 for µg/d data). As iodine intake increased from medium to high, all of the increased iodine was excreted ('overflow') through urine and faeces by males, and 89·5 % was excreted by females. Although the high iodine level (63·4 µg/d) might be adequate in males, the corresponding level of 61·6 µg/d in females did not meet optimal requirements. Our findings indicate that a daily iodine intake of approximately half the current recommended nutrient intake (120 µg/d) may satisfy the minimum iodine requirements of young male adults in China, while a similar level is insufficient for females based on the 'iodine overflow' hypothesis.


Assuntos
Ingestão de Alimentos/fisiologia , Iodo/administração & dosagem , Iodo/análise , Necessidades Nutricionais/fisiologia , Adolescente , Adulto , Grupo com Ancestrais do Continente Asiático , China , Dieta/métodos , Relação Dose-Resposta a Droga , Jejum/sangue , Jejum/urina , Fezes/química , Feminino , Voluntários Saudáveis , Humanos , Masculino , Fatores Sexuais , Cloreto de Sódio na Dieta/administração & dosagem , Cloreto de Sódio na Dieta/análise , Testes de Função Tireóidea , Adulto Jovem
19.
Wei Sheng Yan Jiu ; 49(3): 447-457, 2020 May.
Artigo em Chinês | MEDLINE | ID: mdl-32693895

RESUMO

OBJECTIVE: Comparison of liquid chromatography-tandem mass spectrometry(LC-MS/MS) and enzyme-linked immunosorbent assay(ELISA) for the detection of 25(OH)D concentration in human serum and the diagnosis of vitamin D deficiency. METHODS: In the serum pool of "National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention", 500 serum samples of women aged 18 to 45 years old were randomly selected, and 25(OH)D levels were measured by ELISA and LC-MS/MS for the same serum sample, respectively. The LC-MS/MS column was Waters XBridge BEH C_(18)(2. 1 mm×50 mm, 2. 5 µm). The correlation between the two method was tested by correlation analysis, regression analysis and consistency test. The Endocrine Society and Institute of Medicine recommendations were used to determine the deficiency of vitamin D, and the McNemar test, Kappa coefficient and diagnostic test were used to diagnose the consistency of vitamin D deficiency. RESULTS: The regression equation for the 25(OH)D concentration measured by the two method was y_(LC-MS/MS)=-0. 035+1. 007×x_(ELISA)(r=0. 877), and the average deviation between the two was 4. 48% and the intraclass correlation coefficient was 0. 87. The 25(OH)D concentration was less than 12 and 20 ng/mL as a criterion for vitamin D deficiency, and the Kappa coefficients were greater than 0. 60(0. 64 and 0. 74). CONCLUSION: When serum 25(OH)D level was detected by LC-MS/MS and ELISA, the correspondence of the two method was fine. Taking the "gold standard" LC-MS/MS method as a reference, the ELISA method was used to determine human vitamin D deficiency with good sensitivity and specificity, which can be used for the accurate and rapid detection of large-scale population samples.


Assuntos
Espectrometria de Massas em Tandem , Vitamina D , 25-Hidroxivitamina D 2 , Adolescente , Adulto , Cromatografia Líquida , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Pessoa de Meia-Idade , Vitamina D/análogos & derivados , Adulto Jovem
20.
Wei Sheng Yan Jiu ; 49(3): 453-457, 2020 May.
Artigo em Chinês | MEDLINE | ID: mdl-32693896

RESUMO

OBJECTIVE: To study the digestive stability of 5-enolpyruvylshikimate-3-phosphate synthase(EPSPS) protein and phosphinothricina cetyltransferase(PAT) protein in simulated gastric fluid. METHODS: The component of simulated gastric fluid was based on the method of target protein digestive stability in simulative gastric and intestinal in national standard of the People's Republic of China(Published by the Ministry of Agriculture No. 869-2-2007). The test model of stability of different protein to digestion in Simulated Gastric Fluid was established by dodecyl sulfate, sodium salt-polyacrylamide gel electrophoresis(SDS-PAGE)and western blot. The degradation of EPSPS protein and PAT protein in simulated gastric fluid at different digestion time points were analyzed. RESULTS: The experiment showed that EPSPS protein and PAT protein were completely digested within 15 s in simulated gastric fluid, no any remain of protein was detected by SDS-PAGE and Western blot, indicating that EPSPS protein and PAT protein were easily digested in the simulated gastric. CONCLUSION: EPSPS protein and PAT protein do not have immunogenicity after digestion with simulated gastric fluid.


Assuntos
Digestão , Proteínas , Western Blotting , China , Eletroforese em Gel de Poliacrilamida
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