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1.
Se Pu ; 39(9): 1030-1037, 2021 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-34486843

RESUMO

Portable analytical instruments find extensive application in on-site examination because of their significant advantages: these instruments are convenient and easy-to-carry, leading to high time-effectiveness, and involve low reagent consumption. We report a portable micro-liquid chromatograph (p-µLC) that was designed and fabricated in our laboratory. The p-µLC integrates homemade dual large-thrust syringe pumps for delivering the mobile phase, a capillary polymer monolithic column as the stationary phase for the separation of the target analytes, and a specially designed dual-functional optical-fiber microflow-cell for online detection. The dual-thrust syringe pumps can realize isocratic and/or gradient elution as well as reloading of the mobile phase, with flow rates ranging from 0.025 µL/min to 5.6 mL/min and the maximum working pressure of 4.5 MPa. The polymethacrylate based C-18 monolithic column facilitates the separation of small organic molecules and biomacromolecules. A homemade high-power light emission diode (LED) light source and a modified xenon flash lamp are assembled as the light source module. The dual-functional detector consists of an optical fiber microflow-cell with a self-focusing lens and a light-guiding capillary, light source module, and a small-sized grating spectrometer with an output wavelength range of 400-680 nm for the LED light source and 220-700 nm for the xenon flash lamp, enabling online detection of the absorption and fluorescence spectra of the analytes from 220 to 700 nm. A bifurcated optical fiber bundle is prepared and used to connect the light source, microflow-cell, and grating spectrometer so that the incident light leading-in and the fluorescence/scatting light leading-out can be realized simultaneously. The junction end of the bifurcated optical fiber bundles connects to one end of the light path of the microflow-cell, and a straight-through optical fiber connects another end of the microflow-cell. In the UV-Vis absorption mode, the straight-through optical fiber reads out the transmitted light, while in the fluorescence mode, the excitation light beam from the light source irradiates the sample solution in the flow-cell via one branch of the bifurcated optic fiber bundles. The fluorescence leading-out via the other branch of the bifurcated optical fiber bundles in the opposite direction of the excitation light beam is read out by the spectrometer. All the large-thrust syringe pumps and flow-path, capillary monolithic column, and optical fiber mediated flow-cell detection as well as controlling modules are installed in a suitcase with a total weight of less than 8 kg. The p-µLC is powered by DC 12V 3A or 18650 lithium battery pack and controlled by a panel computer with a custom-built windows-based chromatography workstation software for data acquisition. When using the home-made polymethacrylate based C-18 monolithic capillary column (530 µm ID×200 mm in length), the mixed alkylbenzenes can be separated and detected in an isocratic elution mode. The separation efficiency is comparable to that obtained with a commercially available HPLC.


Assuntos
Polímeros , Cromatografia Líquida de Alta Pressão , Cromatografia Líquida , Indicadores e Reagentes
2.
Signal Transduct Target Ther ; 6(1): 340, 2021 09 09.
Artigo em Inglês | MEDLINE | ID: mdl-34504054

RESUMO

As COVID-19 continues to spread rapidly worldwide and variants continue to emerge, the development and deployment of safe and effective vaccines are urgently needed. Here, we developed an mRNA vaccine based on the trimeric receptor-binding domain (RBD) of the SARS-CoV-2 spike (S) protein fused to ferritin-formed nanoparticles (TF-RBD). Compared to the trimeric form of the RBD mRNA vaccine (T-RBD), TF-RBD delivered intramuscularly elicited robust and durable humoral immunity as well as a Th1-biased cellular response. After further challenge with live SARS-CoV-2, immunization with a two-shot low-dose regimen of TF-RBD provided adequate protection in hACE2-transduced mice. In addition, the mRNA template of TF-RBD was easily and quickly engineered into a variant vaccine to address SARS-CoV-2 mutations. The TF-RBD multivalent vaccine produced broad-spectrum neutralizing antibodies against Alpha (B.1.1.7) and Beta (B.1.351) variants. This mRNA vaccine based on the encoded self-assembled nanoparticle-based trimer RBD provides a reference for the design of mRNA vaccines targeting SARS-CoV-2.


Assuntos
Vacinas contra COVID-19 , COVID-19/prevenção & controle , Nanopartículas , SARS-CoV-2/imunologia , Vacinas Sintéticas , Animais , COVID-19/imunologia , COVID-19/patologia , Vacinas contra COVID-19/química , Vacinas contra COVID-19/farmacologia , Chlorocebus aethiops , Feminino , Células HEK293 , Humanos , Camundongos , Camundongos Transgênicos , Nanopartículas/química , Nanopartículas/uso terapêutico , Células Th1/imunologia , Células Th1/patologia , Vacinas Sintéticas/química , Vacinas Sintéticas/imunologia , Células Vero
3.
Pharmacol Res ; 173: 105881, 2021 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-34509631

RESUMO

IBS-D is a functional bowel disease without clear diagnostic markers and exact pathogenesis. Studies have proved that non-coding RNAs participate in IBS-D. However, tRNA-derived small RNAs (tsRNAs), as a new type of non-coding RNAs that are more suitable as markers, remain to be clarified in IBS-D. Hence, we focused on the identification and potential functions of tsRNAs in IBS-D. Intestinal biopsies were obtained from IBS-D patients and healthy volunteers, and twenty-eight differential tsRNAs were screened by high-throughput sequencing. The changes of tiRNA-His-GTG-001, tRF-Ser-GCT-113, and tRF-Gln-TTG-035 by q-PCR in expanded samples were consistent with the sequencing results. Meanwhile, target gene prediction and bioinformatics showed that the three differential tsRNAs may be involved in some key signal pathways, such as GABAergic synapse, tumor necrosis factor-α (TNF-α), etc. Their regulation on target genes were demonstrated through cell experiments and luciferase reporter assays. In addition, the receiver-operating characteristic (ROC) analysis showed that the three tsRNAs all could be used as candidate markers of IBS-D. The correlation analysis indicated they were related to the degree of abdominal pain, abdominal distension, and stool morphology. So, we believe that the abnormal tiRNA-His-GTG-001, tRF-Ser-GCT-113, and tRF-Gln-TTG-035 are related to the clinical symptoms of IBS-D, and can target regulate the important molecules of the brain-gut axis, even could be expected as potential biomarkers for the diagnosis and treatment of IBS-D.

4.
Spectrochim Acta A Mol Biomol Spectrosc ; 265: 120373, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34547685

RESUMO

An approach is developed to remove the interference of moisture from FTIR spectra. The interference arises from two aspects: the fluctuation on the temperature of the HeNe laser and the fluctuation on the transient concentration of moisture in the light - path of an FTIR spectrometer. The temperature fluctuation on the HeNe laser produces a systematic spectral shift between single-beam sample and background spectra, which often makes spectral subtraction method invalid in removing the interference of moisture. Herein, the Carbo similarity metric (the CAB value) is used to reflect the subtle spectral shift. A database of single-beam background spectra is established based on the concept of big-data and the pigeon-hole theory. The spectral shift is corrected by selecting suitable single-beam background spectra from the database to match with the given single-beam sample spectrum according to the CAB value. The interference caused by the fluctuation of the transient concentration of moisture is removed using a comprehensive 2D-COS method. We apply the approach on two polymeric samples to retrieve high-quality spectra and reliable second derivative spectra without the interference of moisture. The present work provides a new opportunity of obtaining the reliable second derivative spectra in the spectral region masked by moisture.

5.
Zhongguo Zhong Yao Za Zhi ; 46(13): 3311-3318, 2021 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-34396750

RESUMO

The effects of water regulation on the biosynthesis of calycosin-7-O-ß-D-glucoside in 2-year-old Astragalus membranaceus var. mongholicus were studied,and the mechanism was explained from the aspects of key enzyme gene expression and antioxidant enzyme system. The content of calycosin-7-O-ß-D-glucoside was determined by HPLC,and the expression levels of six key enzyme genes( PAL,4 CL,CHS,CHI,IFS,13'H) in the synthesis pathway were analyzed by q RT-PCR. The activities of protective enzymes and contents of osmoregulation substances and malondialdehyde were also determined. In the water deficit group,the maximum concentration of calycosin-7-O-ß-D-glucoside was 0. 49 mg·g-1 on the 24 th day of treatment. In the whole water regulation,the water deficit group outweighed the water adequate group in osmoregulation substance and MDA contents. The activities of A. membranaceus var.mongholicus antioxidant enzymes SOD,POD,and CAT increased during the initial period of water regulation,but decreased with time.The expression of PAL,CHS,and 13'H in the water deficit group was at a low level,and the 4 CL had active expression,slightly lower than that in the water adequate group. The expression of CHI and IFS elevated rapidly when water deficit occurred. Correlation analysis showed that the content of calycosin-7-O-ß-D-glucoside was positively correlated with CHI expression( P<0. 01) and IFS expression( P<0. 05). Therefore,water regulation can change the accumulation pattern of calycosin-7-O-ß-D-glucoside,and water deficit may be an effective way to increase its content. CHI and IFS are the key genes in response to water deficit.


Assuntos
Astragalus propinquus , Isoflavonas , Astragalus propinquus/genética , Vias Biossintéticas , Glucosídeos , Água
6.
Anal Chem ; 93(34): 11792-11799, 2021 08 31.
Artigo em Inglês | MEDLINE | ID: mdl-34407610

RESUMO

The exosome has emerged as a promising noninvasive biomarker for the early diagnosis of cancer. Therefore, it is highly desirable to develop simple, inexpensive, and user-friendly biosensors for convenient, sensitive, and quantitative exosome assay. Herein, we developed a simple and cost-efficient electrochemical biosensor by combining a metal-organic framework (MOF)-functionalized paper and a screen-printed electrode (SPE) for portable, ultrasensitive, and quantitative determination of cancer-derived exosomes. In principle, the biosensor relied on recognition of the exosome by Zr-MOFs and aptamer to initiate the hybridization chain reaction (HCR) and the formation of DNAzyme for signal amplification. Benefiting from the high signal amplification ability of HCR, the label-free paper-based biosensor is capable of ultrasensitive exosome assay with a detection limit down to 5 × 103 particles/mL, which is superior to that of most reported methods. Moreover, the proposed paper-based biosensor possessed the advantages of low cost, simple operation, and high sensitivity, making it affordable and deliverable for point-of-care (POC) diagnosis in resource-limited settings.


Assuntos
Técnicas Biossensoriais , Exossomos , Estruturas Metalorgânicas , Técnicas Eletroquímicas , Limite de Detecção , Hibridização de Ácido Nucleico
7.
Nutrients ; 13(8)2021 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-34444921

RESUMO

Vitamin D (VitD) may affect immune system modulation and result in the development of atopic dermatitis (AD). However, published findings have remained controversial. We investigated the association between early-life 25-hydroxyvitamin D (25(OH)D) levels and AD risk at childhood with a birth cohort. The data were obtained from "the Japan Environment and Children's Study (JECS)" and "the Sub-Cohort study of JECS" performed with children aged 2 years. "Liquid chromatography-tandem mass spectrometry" was used to measure VitD. The information on AD was obtained from parents' answers to a questionnaire when their children were aged 3 years. In order to explain the seasonal effects on VitD levels, a deseasonalized continuous variable was further calculated. The logistic regression models were fitted to evaluate the effect of VitD on childhood AD. The study included 4378 children with complete data on VitD and AD. The results from models indicated that low VitD at 2 years was not a risk factor for the development of AD at 3 years, after adjusting for potential confounders. Moreover, there was no U-shape relationship between deseasonalized VitD and childhood AD. Overall, early-life 25(OH)D levels were not link to the increased risk of developing childhood AD.


Assuntos
Dermatite Atópica/epidemiologia , Deficiência de Vitamina D/sangue , Vitamina D/análogos & derivados , Pré-Escolar , Estudos de Coortes , Dermatite Atópica/etiologia , Feminino , Seguimentos , Humanos , Japão/epidemiologia , Modelos Logísticos , Masculino , Estado Nutricional , Razão de Chances , Prevalência , Fatores de Risco , Estações do Ano , Vitamina D/sangue , Deficiência de Vitamina D/complicações
8.
Nat Chem ; 13(10): 982-991, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34373595

RESUMO

Over the past three decades, organocatalysis has emerged as a powerful catalysis platform and has gradually been incorporated into the routine synthetic toolbox to obtain chiral molecules. However, its application in the site- and enantioselective functionalization of inactive aryl C-H bonds remains in its infancy. Here, we present an organocatalyst-controlled para-selective arene C-H functionalization strategy that addresses this issue, which remains an enduring challenge in arene functionalization chemistry. By emulating enzyme catalysis, the chiral phosphoric acid catalyst offers an ideal chiral environment for stereoinduction, and the projecting substituents give control of chemo- and site-selectivity. Various types of nucleophile are compatible with this method, affording more than 100 para-selective adducts with stereodefined carbon centres or axes in viable molecular contexts. This protocol is expected to provide a general strategy for para-selective functionalization of arene C-H bonds in a controlled manner.

9.
J Immunol ; 207(2): 408-420, 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-34193600

RESUMO

Type I IFNs (IFN-I) are important for tumor immune surveillance and contribute to the therapeutic responses for numerous treatment regimens. Nevertheless, certain protumoral activities by IFN-I have been increasingly recognized. Indeed, our recent work showed that systemic poly(I:C)/IFN treatment can undesirably trigger high arginase (ARG1) expression within the tumor-associated monocyte/macrophage compartment. Using a line of CRISPR-generated Arg1-YFP reporter knock-in mice, we have determined that a subset of tumor-associated macrophages represent the major Arg1-expressing cell type following poly(I:C)/IFN stimulation. More detailed analyses from in vitro and in vivo models demonstrate a surprising IFN-to-IL-4 cytokine axis in transitional monocytes, which can subsequently stimulate IL-4 target genes, including Arg1, in macrophages. Intriguingly, IFN stimulation of transitional monocytes yielded concurrent M2 (YFP+)- and M1 (YFP-)-skewed macrophage subsets, correlated with an inhibitory crosstalk between IFN-I and IL-4. Genetic abrogation of IL-4 signaling in mice diminished poly(I:C)/IFN-induced ARG1 in tumors, leading to enhanced activation of CD8+ T cells and an improved therapeutic effect. The present work uncovered a monocyte-orchestrated macrophage phenotype conversion mechanism that may have broad implications.

10.
Medicine (Baltimore) ; 100(27): e26535, 2021 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-34232190

RESUMO

BACKGROUND: Accumulating studies have focused on the clinicopathological and prognostic roles of large intergenic noncoding RNA regulator of reprogramming (lincRNA-ROR) in cancer patients. However, the results were controversial and unconvincing. Thus, we performed a meta-analysis to assess the associations between lincRNA-ROR expression and survival and clinicopathological characteristics of cancer patients. METHODS: Hazard ratios for overall survival and disease-free survival with their 95% confidence intervals were used to evaluate the role of lincRNA-ROR expression in the prognosis of cancer patients. Risk ratios with their 95% confidence intervals were applied to assess the relationship between lincRNA-ROR expression and clinicopathological parameters. RESULTS: A total of 18 articles with 1441 patients were enrolled. Our results indicated that high lincRNA-ROR expression was significant associated with tumor size, TNM stage, clinical stage, lymph metastasis, metastasis and vessel invasion of cancer patients. There were no correlations between high lincRNA-ROR expression and age, gender, infiltration depth, differentiation, serum CA19-9 and serum CEA of cancer patients. In addition, high lincRNA-ROR expression was associated with shorter Overall survival and disease-free survival on both univariate and multivariate analyses. Meanwhile, there were no obvious publication bias in our meta-analysis. CONCLUSIONS: LincRNA-ROR expression was associated with the clinicopathological features and outcome of cancer patients, which suggested that lincRNA-ROR might serve as a potential biomarker for cancer prognosis. ETHICAL APPROVAL: Since this study is on the basis of published articles, ethical approval and informed consent of patients are not required.


Assuntos
Regulação Neoplásica da Expressão Gênica , Neoplasias/genética , RNA Longo não Codificante/genética , Biomarcadores Tumorais/biossíntese , Biomarcadores Tumorais/genética , Linhagem Celular Tumoral , Humanos , Neoplasias/metabolismo , Prognóstico
11.
J Am Chem Soc ; 143(24): 8993-9001, 2021 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-34106720

RESUMO

The enantioselective synthesis of axially chiral biaryls by a copper-catalyzed Diels-Alder/retro-Diels-Alder reaction of 2-pyrones with alkynes is reported herein. Using electron-deficient 2-pyrones and electron-rich 1-naphthyl acetylenes as the reaction partners, a broad range of axially chiral biaryl esters are obtained in excellent yields (up to 97% yield) and enantioselectivities (up to >99% ee). DFT calculations reveal the reaction mechanism and provide insights into the origins of the stereoselectivities. The practicality and robustness of this reaction are showcased by gram-scale synthesis. The synthetic utilizations are demonstrated by the amenable transformations of the products.

12.
Innovation (N Y) ; : 100140, 2021 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-34179862

RESUMO

A safe and effective vaccine is critical to combat the COVID-19 pandemic. Here, we developed a trimeric SARS-CoV-2 receptor-binding domain (RBD) subunit vaccine candidate that simulates the natural structure of the spike (S) trimer glycoprotein. Immunization with the RBD-trimer induced robust humoral and cellular immune responses, and a high level of neutralizing antibodies was maintained for at least 4.5 months. Moreover, the antibodies that were produced in response to the vaccine effectively cross-neutralized the SARS-CoV-2 501Y.V2 variant (B.1.351). Of note, when the vaccine-induced antibodies dropped to a sufficiently low level, only one boost quickly activated the anamnestic immune response, conferring full protection against a SARS-CoV-2 challenge in rhesus macaques without typical histopathological changes in the lung tissues. These results demonstrated that the SARS-CoV-2 RBD-trimer vaccine candidate is highly immunogenic and safe, providing long-lasting, broad, and significant immunity protection in nonhuman primates, thereby offering an optimal vaccination strategy against COVID-19.

13.
Nutrients ; 13(3)2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33804474

RESUMO

Few epidemiologic studies have examined the role of maternal iron status in allergic diseases in offspring and findings have been inconsistent. We used a large birth cohort in Japan to explore the association of the markers for maternal iron status (maternal hemoglobin, hematocrit and dietary iron intake during pregnancy) with allergy development in offspring during early childhood. We analyzed information on children age 0-3 years from the Japan Environment and Children's Study (JECS). We used logistic models and generalized estimating equation models to evaluate the effect of maternal hemoglobin and hematocrit levels and dietary iron intake on allergies in children. Models were also fitted with propensity score-matched datasets. Data were collected for a total of 91,247 mother-child pairs. The prevalence (95% confidence interval) of low hemoglobin and hematocrit was 14.0% (13.7-14.2%) and 12.5% (12.3-12.8%), respectively. After adjusting confounders, low hemoglobin and hematocrit during pregnancy were not associated with childhood allergic outcomes. Findings from models with propensity score-matched datasets also indicated that children born to mothers with low hemoglobin or hematocrit levels during pregnancy did not have a higher risk of developing allergic conditions at 3 years old. We found no meaningful associations between low energy adjusted maternal dietary iron intake and allergies in children. In conclusion, using birth cohort data, we found no evidence supporting an association of low maternal hemoglobin, hematocrit and low dietary iron intake with allergy symptoms during early childhood. Further studies with more suitable proxy markers for blood iron status are needed.


Assuntos
Ingestão de Alimentos/fisiologia , Hipersensibilidade/etiologia , Ferro na Dieta/análise , Fenômenos Fisiológicos da Nutrição Materna , Efeitos Tardios da Exposição Pré-Natal/etiologia , Pré-Escolar , Feminino , Hematócrito , Hemoglobinas , Humanos , Hipersensibilidade/epidemiologia , Lactente , Recém-Nascido , Japão/epidemiologia , Masculino , Gravidez , Trimestres da Gravidez/sangue , Efeitos Tardios da Exposição Pré-Natal/epidemiologia
14.
ACS Chem Neurosci ; 12(7): 1093-1099, 2021 04 07.
Artigo em Inglês | MEDLINE | ID: mdl-33764738

RESUMO

ß-Site amyloid protein cleaving enzyme 1 (BACE1) is a promising therapeutic target for developing inhibitors to alleviate Alzheimer's disease (AD). Herein, we established an inductively coupled plasma mass spectrometry (ICPMS)-based inhibitor screening platform. A biotin-labeled lanthanide-coded peptide probe (LCPP; biotin-PEG2-EVNLDAEC-DOTA-Ln) was designed to determine the activity of BACE1 and evaluate the degree of inhibition of inhibitors. The platform was first validated with two commercially available inhibitors (BSI I and BSI IV) in terms of IC50 values and then applied to two newly designed inhibitors (inhibitors II and III) based on the crystal structure of BACE1 interacting with inhibitor I, and each of them contained an acylguanidine core structure. We found that their inhibition effects were improved as evaluated by the sensitive and accurate LCPP-ICPMS platform, demonstrating its ability for new drug screening.


Assuntos
Doença de Alzheimer , Peptídeos beta-Amiloides , Doença de Alzheimer/tratamento farmacológico , Secretases da Proteína Precursora do Amiloide , Ácido Aspártico Endopeptidases , Inibidores Enzimáticos/farmacologia , Humanos , Espectrometria de Massas
15.
Inorg Chem ; 2021 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-33710864

RESUMO

Single-crystal structures of five lanthanide-erythritol complexes are reported. The analysis of the chemical compositions and scrutinization of structural features in the single-crystal data of the complexes led us to find that unexpected deprotonation occurs on the OH group of erythritol of three complexes. Considering these complexes were prepared in acidic environments, where spontaneous ionization on an OH group is suppressed, we suggest metal ions play an important role in promoting the proton transfer. To find out why the chemically inert OH is activated, the single-crystal structures of 63 rare-earth complexes containing organic ligands with multiple hydroxyl groups (OLMHs) were surveyed. The formation of µ2-bridges turns out to be directly relevant to the occurrence of deprotonation. When an OH group from an OLMH molecule participates in the formation of a µ2-bridge, the polarization ability of the metal ions becomes strong enough to promote the deprotonation on the OH group. The above structural characteristics may be useful in the rational design of catalysts that can activate the chemically inert OH group and promote the relevant chemical conversions.

16.
Sheng Wu Gong Cheng Xue Bao ; 37(2): 673-679, 2021 Feb 25.
Artigo em Chinês | MEDLINE | ID: mdl-33645165

RESUMO

Nucleic acid detection technique has good sensitivity and specificity and is widely used in in vitro diagnosis, animal and plant commodity quarantine, forensic identification, and other fields. However, it is susceptible to carryover contamination during the operation and leads to false-positive results, which seriously affects the detection accuracy. Therefore, finding an effective solution to prevent and eliminate nucleic acid carryover contamination has become particularly urgent. This study compared several different methods for removing nucleic acid contamination and confirmed that sodium hypochlorite solution and PCRguard reagent could effectively eliminate nucleic acid carryover in the liquid and on surfaces of different materials. Besides, the combination of sodium hypochlorite solution and PCRguard can solve the nucleic acid aerosol contamination. This study proposes solutions for the routine prevention of carryover contamination and removal of aerosol that has occurred in molecular diagnostic laboratories.


Assuntos
Ácidos Nucleicos , Laboratórios , Patologia Molecular
17.
Int J Clin Exp Pathol ; 14(3): 383-388, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33786156

RESUMO

BACKGROUND: Pigmented villonodular synovitis (PVNS) is a rare benign proliferative disorder of the synovium. It rarely occurs in adolescents, particularly in immature patients with bilateral manifestation. CASE PRESENTATION: We present a case of atypical and bilateral PVNS of wrist in a 14-year-old boy. Initially, the patient presented with left wrist pain and swelling without the history of trauma. Physical examination revealed an obvious lesion in the dorsal part of left wrist. Radiographs, computed tomography (CT) and magnetic resonance imaging (MRI) showed multiple abnormal signal shadows and arthroedema in the left wrist. Arthroscopy operation was performed, and histologic examination suggested the diagnosis of PVNS. Only 10 months later, the patient presented with the similar symptoms and signs in the right wrist. But MIR and histologic examination were atypical. In this article, we also review and summarize 26 studies on 30 adolescent patients with PVNS. CONCLUSIONS: This study provides an example of atypical and bilateral PVNS in adolescents.

18.
PLoS Negl Trop Dis ; 15(3): e0008403, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33711011

RESUMO

Ebola virus (EBOV) is one of the most virulent pathogens that causes hemorrhagic fever and displays high mortality rates and low prognosis rates in both humans and nonhuman primates. The post-exposure antibody therapies to prevent EBOV infection are considered effective as of yet. However, owing to the poor thermal stability of mammalian antibodies, their application in the tropics has remained limited. Therefore, a thermostable therapeutic antibody against EBOV was developed modelled on the poultry(chicken) immunoglobulin Y (IgY). The IgY antibodies retaining their neutralising activity at 25°C for one year, displayed excellent thermal stability, opposed to conventional polyclonal antibodies (pAbs) or monoclonal antibodies (mAbs). Laying hens were immunised with a variety of EBOV vaccine candidates and it was confirmed that VSVΔG/EBOVGP encoding the EBOV glycoprotein could induce high titer neutralising antibodies against EBOV. The therapeutic efficacy of immune IgY antibodies in vivo was evaluated in the newborn Balb/c mice who have been challenged with the VSVΔG/EBOVGP model. Mice that have been challenged with a lethal dose of the pseudovirus were treated 2 or 24 h post-infection with different doses of anti-EBOV IgY. The group receiving a high dose of 106 NAU/kg (neutralising antibody units/kilogram) showed complete protection with no symptoms of a disease, while the low-dose group was only partially protected. Conversely, all mice receiving naive IgY died within 10 days. In conclusion, the anti-EBOV IgY exhibits excellent thermostability and protective efficacy. Anti-EBOV IgY shows a lot of promise in entering the realm of efficient Ebola virus treatment regimens.


Assuntos
Anticorpos Antivirais/uso terapêutico , Ebolavirus/imunologia , Doença pelo Vírus Ebola/prevenção & controle , Imunoglobulinas/uso terapêutico , Profilaxia Pós-Exposição , Transferência Adotiva , Animais , Anticorpos Neutralizantes/imunologia , Anticorpos Antivirais/imunologia , Linhagem Celular , Galinhas/imunologia , Chlorocebus aethiops , Cricetinae , Vacinas contra Ebola/imunologia , Feminino , Cobaias , Células HEK293 , Humanos , Imunoglobulinas/administração & dosagem , Imunoglobulinas/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Vacinação , Células Vero , Proteínas do Envelope Viral/imunologia
19.
Int Arch Allergy Immunol ; 182(7): 650-662, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33601376

RESUMO

INTRODUCTION: Previous studies have reported that maternal asthma increases the risk of preterm birth. We hypothesized that inflammatory reactions caused by allergic diseases might affect the uterine environment and, subsequently, perinatal outcomes. The objective of this study was to examine the associations between allergic features among mothers and preterm pregnancy outcomes in a nationwide birth cohort. METHODS: We analyzed data from pregnant women obtained from the Japanese Environment and Children's Study (JECS), a nationwide general birth cohort study. We used binomial and multinomial logistic regression models to examine the associations between maternal allergic features and preterm birth, threatened preterm labor (TPL), and preterm premature rupture of the membrane (PPROM). RESULTS: A total of 97,683 pregnant women were included. Prevalence of preterm birth, TPL, and PPROM was 4.7, 19.6, and 1.2%, respectively. Maternal history of allergic diseases (asthma, allergic rhinitis, allergic conjunctivitis, food allergy, drug allergy, and contact dermatitis) increased the risk of TPL(adjusted odds ratio [aOR] = 1.11 [95% CI: 1.06-1.17], aOR = 1.12 [1.08-1.16], aOR = 1.10 [1.04-1.16], aOR = 1.17 [1.09-1.26], aOR = 1.35 [1.23-1.48], and aOR = 1.34 [1.20-1.49], respectively). Although some maternal allergic features showed a negative association with preterm birth, the variables affecting preterm birth differed according to the gestational age of the fetus (22-33 weeks vs. 34-36 weeks). There were no significant associations between maternal allergic features and PPROM. CONCLUSION: Maternal allergic disease, except atopic dermatitis, may increase the risk of TPL. Comorbidity of maternal allergic disorders and perinatal adverse outcomes require further investigation.


Assuntos
Hipersensibilidade/epidemiologia , Hipersensibilidade/etiologia , Exposição Materna/efeitos adversos , Resultado da Gravidez , Nascimento Prematuro/epidemiologia , Efeitos Tardios da Exposição Pré-Natal , Feminino , Humanos , Lactente , Recém-Nascido , Japão/epidemiologia , Razão de Chances , Gravidez , Nascimento Prematuro/etiologia , Vigilância em Saúde Pública
20.
Anal Chim Acta ; 1150: 238118, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-33583540

RESUMO

A syringe-aided apta-nanosensing method is reported for the colorimetric determination of acetamiprid. The method employs double-stranded (ds) DNA-conjugated gold nanoparticle@magnetic agarose beads, i.e., dsDNA-AuNP@MABs as peroxidase-mimicking composite probes, in which the aptamer is indirectly attached to the AuNP surface through its hybridization with complementary DNA (cDNA). Upon contact with the acetamiprid target, the probes can give perceptible color change due to the possible conformation switch from dsDNA's brush-like to cDNA's 'pancake' regime. An "air-spaced pumping" procedure using a syringe equipped with ring magnets as the operation platform was proposed to facilitate the magnetic separation of the sensing probes. Therefore, the analytical steps can be easily accomplished in a syringe, including probe loading, acetamiprid capture and magnetic separation from crude samples, chromogenic reagent loading and colorimetric visualization. Acetamiprid concentration down to 3.3 ppb can be easily identified by the naked eye. The final solution also can be transferred for quantitative measurement. Under spectrometer, the ratio of the absorbance at 652 nm in the presence and absence of acetamiprid (A/A0) is linearly related to the acetamiprid concentration in the 0.4-4.5 ppb range. The limit of detection is calculated to be 0.24 ppb. Moreover, satisfactory recoveries ranging from 90.90 to 91.82% with relative standard deviations of ≤2.96% were obtained in analyzing real spiked samples.


Assuntos
Aptâmeros de Nucleotídeos , Técnicas Biossensoriais , Nanopartículas Metálicas , Colorimetria , Ouro , Neonicotinoides , Seringas
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