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1.
Environ Int ; 171: 107741, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36628860

RESUMO

BACKGROUND: It remains unclear whether type 2 diabetes and the complication of arthritis are causally related to the PM2.5 pollutant. Therefore, we aimed to investigate the associations of long-term PM2.5 exposure with type 2 diabetes and with arthritis in type 2 diabetes patients. MATERIALS AND METHODS: This study used data from the China Health and Retirement Longitudinal Survey (CHARLS) implemented during 2011-2018. The associations were analyzed by Cox proportional hazards regression models, and the population-attributable fraction (PAF) was calculated to assess the burden of type 2 diabetes and arthritis-attributable to PM2.5. RESULTS: A total of 21,075 participants were finally included, with 19,121 analyzed for PM2.5 and type 2 diabetes risk and 12,427 analyzed for PM2.5 and arthritis risk, of which 1,382 with newly-diagnosed type 2 diabetes and 1,328 with arthritis during the follow-up. Overall, each 10 µg/m3 increment in PM2.5 concentration was significantly associated with an increase in the risk of type 2 diabetes (HR = 1.26, 95 %CI1.22 to 1.31), and the PAF of type 2 diabetes attributable to PM2.5 was 13.54 %. In type 2 diabetes patients, each 10 µg/m3 increment in PM2.5 exposure was associated with an increase in arthritis (HR = 1.42, 95 %CI: 1.28 to 1.57), and the association was significantly greater than that (H = 1.23, 95 %CI: 1.19 to 1.28) in adults without type 2 diabetes. The PAFs of arthritis-attributable to PM2.5 in participants with and without type 2 diabetes were 18.54 % and 10.69 %, respectively. CONCLUSION: Long-term exposure to PM2.5 may increase the risk of type 2 diabetes and make type 2 diabetes patients susceptible to arthritis.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Diabetes Mellitus Tipo 2 , Adulto , Humanos , Diabetes Mellitus Tipo 2/epidemiologia , Diabetes Mellitus Tipo 2/etiologia , Poluentes Atmosféricos/efeitos adversos , Poluentes Atmosféricos/análise , Material Particulado/efeitos adversos , Material Particulado/análise , Exposição Ambiental/análise , China/epidemiologia
2.
Ecotoxicol Environ Saf ; 250: 114502, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36603489

RESUMO

Thyroid hormones are essential for fetal growth and neurodevelopment. The recent frequent use of parabens has raised concerns about their endocrine-disrupting potential. However, the effects of maternal paraben exposure on neonatal thyroid hormone levels are still largely unknown. In our study, a co-twin control design was employed to analyze the relationships between maternal paraben exposure and neonatal thyroid-stimulating hormone (TSH) difference. We collected information from 252 mother-twin pairs from a twin birth cohort in Wuhan, China. Concentrations of six parabens were measured in maternal urine samples collected at < 16, 16-28, and > 28 weeks of gestation. Data of neonatal TSH levels were retrieved from medical records. Multiple informant models were applied to explore the time-specific relationships between paraben exposure and intra-twin TSH difference and to determine the susceptible window of exposure. We found that maternal urinary methyl paraben (MeP) during early pregnancy was positively associated with intra-twin TSH difference (%change = 5.96 %; 95 % confidant interval (CI): 0.04 %, 12.2 %). However, no significant differences were observed for exposure to ethyl paraben (EtP) and propyl paraben (PrP), and the associations between parabens and intra-twin TSH difference did not differ materially across pregnancy. Further, a stratified analysis based on twin zygosity and chorionicity and sex types indicated that the positive association between early pregnancy MeP exposure and intra-twin TSH difference was significant in monochorionic diamniotic (MCDA) twins of female-female fetuses and dichorionic diamniotic (DCDA) twins of opposite-sex. The prospective twin study provides first evidence that MeP exposure in early pregnancy was associated with an increased TSH difference in twin neonates, especially in female fetuses.


Assuntos
Exposição Materna , Parabenos , Tireotropina , Feminino , Humanos , Recém-Nascido , Gravidez , Exposição Materna/efeitos adversos , Parabenos/toxicidade , Parabenos/análise , Estudos Prospectivos , Hormônios Tireóideos , Tireotropina/sangue , Gêmeos
3.
Biochim Biophys Acta Mol Basis Dis ; : 166657, 2023 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-36716897

RESUMO

Mirror image pain (MIP), a clinical syndrome of contralateral pain hypersensitivity caused by unilateral injury, has been identified in various neuropathological conditions. Gap junctional protein Connexin 43 (Cx43), its phosphorylation levels and dopamine D2 receptor (DRD2) play key integrating roles in pain processing. We presume D2DR activity may affect Cx43 hemichannel opening via Cx43 phosphorylation levels to regulate MIP. This study shows that spinal astrocytic Cx43 directly interacts with DRD2 to mediate MIP. DRD2 and Cx43 expression levels were asymmetrically elevated in bilateral spinal during MIP, and DRD2 modulated the opening of primary astrocytic Cx43 hemichannels. Furthermore, Cx43 phosphorylation at Ser373 was increased during MIP, but decreased in DRD2 knockout (KO) mice. Finally, activation of spinal protein kinase A (PKA) altered the expression of Cx43 and its phosphorylation bilaterally, thus reversing the analgesic effect in DRD2 KO mice. Together, these data reveal that spinal Cx43 phosphorylation and channel opening are regulated by DRD2 via PKA activation, and that spinal Cx43 and DRD2 are key molecular sensors mediating mirror image pain.

4.
Sci Total Environ ; : 161472, 2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36638985

RESUMO

Previous studies have reported potential adverse effects of exposure to ambient air pollutants on semen quality in infertile men, but studies on the general population have been limited and inconsistent, and the pollutants that play a major role remain unclear. This study aimed to explore the potential association between exposure to six air pollutants (PM2.5, PM10, NO2, SO2, O3 and CO) during different sperm development periods and semen quality among the general population, and to explore the interaction between different air pollutant exposures. We included 1515 semen samples collected from the Human Sperm Bank. We improved individuals' exposure level estimation by combining inverse distance weighting (IDW) interpolation with satellite remote sensing data. Multivariate linear regression models, restricted cubic spline functions and double-pollutant models were used to assess the relationship between exposure to six air pollutants and sperm volume, concentration, total sperm number and sperm motility. A negative association was found between SO2 exposure and progressive motility and total motility during 0-90 lag days and 70-90 lag days, and SO2 exposure during 10-14 lag days adversely affected sperm concentration and total sperm number. Sensitive analyses for qualified sperm donors and the double-pollutant models obtained similar results. Additionally, there were nonlinear relationships between exposure to PM, NO2, O3, CO and a few semen parameters, with NO2 and O3 exposure above the threshold showing negative correlations with total motility and progressive motility, respectively. Our study suggested that SO2 may play a dominant role in the adverse effects of ambient air pollutants on semen quality in the general population by decreasing sperm motility, sperm concentration and total sperm number, and even SO2 exposure lower than the recommended value of the WHO can still cause male reproductive toxicity, which deserves attention.

5.
J Environ Manage ; 329: 116996, 2023 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-36535143

RESUMO

A critical step in water management policy development is the analysis of its socio-economic and environmental implications. However, few methods could proactively and reliably predict and assess the impacts of policies while handling the inherent uncertainty. To fill such a gap, an inexact simulation-evaluation method was developed for analyzing the impacts of multiple water management policies under uncertainty. The interval positive mathematical programming (IPMP) method was proposed as the simulation tool by coupling interval programming with positive mathematical programming (PMP). The evaluation tool was developed by combining the interval TOPSIS method and the interval maximum deviation method. This simulation-evaluation method can directly communicate a policy's simulation outcomes into the evaluation process while addressing the uncertainties in both simulation and evaluation. The proposed method can also reproduce the actual situation with a calibration process, which enables accurate and smooth responses to policy changes. This approach was used for agricultural water management in arid north-west China. Seventy-five policy alternatives generated from three groundwater allocation limits and twenty-five differential water pricing levels were investigated. The impacts of these alternatives on farmer income, farmer employment, water consumption, planting areas, and fertilizer use were simulated using IPMP. Twenty-four non-inferior alternatives were selected and further evaluated with multi-dimensional criteria. The final results showed that, the water price for grain crops with traditional irrigation methods should rise by 60%, those for cash crops with drip irrigation should decrease by 60%, and the groundwater quota should be reduced by 20%. Compared with traditional models, IPMP can increase simulation accuracy by reproducing observed situations, enhance robustness by reflecting input uncertainty, and improve flexibility in decision-making by providing interval solutions. The inexact simulation-evaluation model can also be widely used to analyze other policies.


Assuntos
Água Subterrânea , Água , Modelos Teóricos , Incerteza , Custos e Análise de Custo
6.
Environ Int ; 171: 107698, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36535190

RESUMO

BACKGROUND: Polycyclic aromatic hydrocarbons (PAHs) have been shown to disrupt thyroid function in toxicological studies, but epidemiological evidence is inconsistent. Furthermore, little is known on potential effects of mixtures of PAHs. OBJECTIVE: This study aimed to examine the associations of exposure to PAHs as individual chemicals and mixtures with thyroid hormones. METHODS: We included 378 men from a Reproductive Medicine Center in Wuhan, China. Ten monohydroxylated PAH (OH-PAH) metabolites in repeated urine specimens collected at two-time points and three thyroid hormones [thyroid-stimulating hormone (TSH), free thyroxine (FT4), and free triiodothyronine (FT3)] in one serum sample were measured. Multivariable linear regression models were applied to assess the associations between individual OH-PAH metabolites and thyroid hormones, and the associations with mixtures of OH-PAH metabolites were assessed by Bayesian kernel machine regression (BKMR) models. RESULTS: Multivariable linear regression models showed inverse associations between urinary 1-OHNa and TSH, between urinary 1-OHPh and 9-OHPh and FT3, as well as between urinary 2-OHPh, 3-OHPh, 9-OHPh and ∑OHPh and FT4, regardless of these individual OH-PAH metabolites modeled as continuous or tertile variables (e.g., -21.57 % in TSH; 95 % CI: -35.33 %, -4.88 % for the third vs first tertiles of 1-OHNa; p for trend = 0.014). BKMR models showed negative overall effects of all urinary OH-PAH metabolite mixtures on TSH, FT3, and FT4, and 1-OHNa, 9-OHPh, and 2-OHPh as the most important contributors, respectively, with linear inverse exposure-response associations when holding other OH-PAH metabolites at their median concentrations. CONCLUSION: Urinary OH-PAH metabolites as individual chemicals and mixtures were adversely associated with thyroid hormones among reproductive-aged men.


Assuntos
Hidrocarbonetos Policíclicos Aromáticos , Masculino , Humanos , Adulto , Hidrocarbonetos Policíclicos Aromáticos/urina , Teorema de Bayes , Hormônios Tireóideos , Reprodução , Tireotropina
7.
J Environ Manage ; 329: 117016, 2023 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-36586328

RESUMO

The environmental damage caused by surface subsidence and coal-based solid waste (CBSW) is a common problem in the process of coal mining. Backfill mining can control the mining-induced subsidence and solve the problem of bulk solid waste storage. In the present work, a magnesium-coal slag solid waste backfill material (MCB) with modified magnesium slag (MS) as binder and CBSW (fly ash (FA), flue gas desulfurization gypsum (FDG) and coal gasification slag (CGS)) as supplementary cementitious material/aggregate was proposed to meet the needs of coal mining in Northern Shaanxi, China, to realize the comprehensive treatment of goaf and CBSW. The results show that: (1) The rheological curve of the fresh MCB slurry is highly consistent with the Herschel-Bulkley (H-B) model, and its fluidity meets the basic requirements of mine backfill pumping. With the addition of FDG and MS, the yield stress, apparent viscosity and thixotropy of MCB slurry increase, while the pseudoplastic index and slump decrease. (2) The strength of MCB develops slowly in the early stage (0∼14 days) and increases rapidly in the later stage (14∼90 days). Except for the ratio of M20F1 and FDG = 0%, the strength of samples at other ratios (at 28 days) is between 6.06∼11.68 MPa, which meets the strength requirement of 6 MPa for coal mine backfill. The addition of MS and appropriate amount of FDG is beneficial to the development of strength. In contrast, MS exhibits a significant improvement in early strength, and FDG has a significant improvement in late-age strength. (3) Corresponding to the compressive strength, the hydration products C-S(A)-H and AFt of MCB are less in the early stage and greatly increased in the later stage. The active substance in FA/CGS will undergo pozzolanic reaction with the MS hydration product CH. The addition of FDG and MS can promote the reaction and increase the amount of hydration product, but in contrast, the promotion effect of FDG is more significant. (4) The amount of heavy metal leaching of MCB meets the requirements of national standards. The hardened MCB has a solidification/stabilization effect on heavy metal elements, which can significantly reduce the amount of heavy metal leaching. The results imply that MCB is a safe, reliable, and eco-friendly solid waste backfill material, and its application is conducive to the coordinated development of coal resource mining and environmental protection.


Assuntos
Minas de Carvão , Metais Pesados , Magnésio , Resíduos Sólidos , Carvão Mineral/análise , Fluordesoxiglucose F18 , Minas de Carvão/métodos , Cinza de Carvão
8.
Neural Regen Res ; 18(5): 1090-1098, 2023 May.
Artigo em Inglês | MEDLINE | ID: mdl-36254998

RESUMO

Neural progenitor cells (NPCs) capable of self-renewal and differentiation into neural cell lineages offer broad prospects for cell therapy for neurodegenerative diseases. However, cell therapy based on NPC transplantation is limited by the inability to acquire sufficient quantities of NPCs. Previous studies have found that a chemical cocktail of valproic acid, CHIR99021, and Repsox (VCR) promotes mouse fibroblasts to differentiate into NPCs under hypoxic conditions. Therefore, we used VCR (0.5 mM valproic acid, 3 µM CHIR99021, and 1 µM Repsox) to induce the reprogramming of rat embryonic fibroblasts into NPCs under a hypoxic condition (5%). These NPCs exhibited typical neurosphere-like structures that can express NPC markers, such as Nestin, SRY-box transcription factor 2, and paired box 6 (Pax6), and could also differentiate into multiple types of functional neurons and astrocytes in vitro. They had similar gene expression profiles to those of rat brain-derived neural stem cells. Subsequently, the chemically-induced NPCs (ciNPCs) were stereotactically transplanted into the substantia nigra of 6-hydroxydopamine-lesioned parkinsonian rats. We found that the ciNPCs exhibited long-term survival, migrated long distances, and differentiated into multiple types of functional neurons and glial cells in vivo. Moreover, the parkinsonian behavioral defects of the parkinsonian model rats grafted with ciNPCs showed remarkable functional recovery. These findings suggest that rat fibroblasts can be directly transformed into NPCs using a chemical cocktail of VCR without introducing exogenous factors, which may be an attractive donor material for transplantation therapy for Parkinson's disease.

9.
Materials (Basel) ; 15(24)2022 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-36556652

RESUMO

Spodumene tailing is the associated solid waste of extracting lithium from spodumene. With the increase in the global demand for lithium resources, its emissions increase yearly, which will become a key factor restricting the economic development of the mining area. Mechanical and hydration reactions, as well as the microstructure of early CSTB, are studied under different tailings-cement ratios (TCR) and solid mass concentration (SC) conditions. The results show that the uniaxial compressive strength of early CSTB has a negative exponential correlation with the decrease in TCR and a positive correlation with the increase in SC: when the age of CSTB increases to 7 days, the strength increases with the rise in SC in an exponential function, and the sensitivity of strength to TCR is higher than that of SC. Compared to other tailings cemented backfill materials, the addition of spodumene tailings reduces the sulfate ion concentration and leads to a new exothermic peak (i.e., the third exothermic peak) for the hydration exotherm of CSTB. Additionally, with the increase in TCR or decrease in SC, the height of the third exothermic peak decreases and the occurrence time is advanced. At the same time, the duration of induction phase was prolonged, the period of acceleration phase was shortened, and the total amount of heat released was significantly increased. The decrease in TCR or the increase in SC led to the rise in the number of hydration products which can effectively fill the internal pores of CSTB, enhance its structural compactness, and increase its compressive strength. The above study reveals the influence of TCR and SC on the early strength, hydration characteristics, and microstructure of CSTB and provides an essential reference for the mix design of underground backfill spodumene tailings.

10.
Biomed Res Int ; 2022: 4909544, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36578802

RESUMO

Objective: DNA damage response (DDR) is a complex system that maintains genetic integrity and the stable replication and transmission of genetic material. m6A modifies DDR-related gene expression and affects the balance of DNA damage response in tumor cells. In this study, a risk model based on m6A-modified DDR-related gene was established to evaluate its role in patients with gastric cancer. Methods: We downloaded 639 DNA damage response genes from the Gene Set Enrichment Analysis (GSEA) database and constructed risk score models using typed differential genes. We used Kaplan-Meier curves and risk curves to verify the clinical relevance of the model, which was then validated with the univariate and multifactorial Cox analysis, ROC, C-index, and nomogram, and finally this model was used to evaluate the correlation of the risk score model with immune microenvironment, microsatellite instability (MSI), tumor mutational burden (TMB), and immune checkpoints. Results: In this study, 337 samples in The Cancer Genome Atlas (TCGA) database were used as training set to construct a DDR-related gene model, and GSE84437 was used as external data set for verification. We found that the prognosis and immunotherapy effect of gastric cancer patients in the low-risk group were significantly better than those in the high-risk group. Conclusion: We screened eight DDR-related genes (ZBTB7A, POLQ, CHEK1, NPDC1, RAMP1, AXIN2, SFRP2, and APOD) to establish a risk model, which can predict the prognosis of gastric cancer patients and guide the clinical implementation of immunotherapy.


Assuntos
Neoplasias Gástricas , Humanos , Neoplasias Gástricas/genética , Neoplasias Gástricas/terapia , Linhagem Celular Tumoral , Proteínas de Ligação a DNA , Fatores de Transcrição , Prognóstico , Dano ao DNA/genética , Microambiente Tumoral
11.
China CDC Wkly ; 4(41): 912-919, 2022 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-36426289

RESUMO

Introduction: Although helminth infections threaten millions of people worldwide, the spatiotemporal characteristics remain unclear across China. This study systematically describes the spatiotemporal changes of major human helminth infections and their epidemiological characteristics from 1988 to 2021 in Guangdong Province, China. Methods: The survey data in Guangdong Province were primarily obtained from 3 national surveys implemented during 1988-1992, 2001-2004, and 2014-2016, respectively, and from the China Information System for Disease Control and Prevention during 2019-2021. A modified Kato-Katz technique was used to detect parasite eggs in collected fecal samples. Results: The overall standardized infection rates (SIRs) of any soil-transmitted helminths (STH) and Clonorchis sinensis decreased from 65.27% during 1988-1992 to 4.23% during 2019-2021. In particular, the SIRs of STH had even more of a decrease, from 64.41% during 1988-1992 to 0.31% during 2019-2021. The SIRs of Clonorchis sinensis in the 4 surveys were 2.40%, 12.17%, 5.20%, and 3.93%, respectively. This study observed different permutations of gender, age, occupation, and education level on the SIRs of helminths. Conclusions: The infection rate of STH has substantially decreased. However, the infection rate of Clonorchis sinensis has had fewer changes, and it has become the dominant helminth.

12.
Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi ; 36(11): 869-871;874, 2022 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-36347582

RESUMO

Objective:To evaluate the effect of body mass index (BMI) on efficacy of the repositioning procedure for horizontal canal benign paroxysmal positional vertigo (HC-BPPV). Methods:A total of 172 patients diagnosed with HC-BPPV in the First Affiliated Hospital of Xi'an Jiaotong University School of Medicine from Jan 2021 to Oct 2021 were enrolled. The patients were divided into low BMI group(BMI<24 kg/m² ) and high BMI group(BMI≥24 kg/m²ï¼‰. The two groups underwent manual repositioning procedure and vertigo treatment system procedure by Barbecue method according to their orders of visits. After two circles of repositioning, the influencing factors of repositioning were analyzed according to efficiency. Results:The effective rate of manual repositioning in HC-BPPV patients was 33.64%, and the effective rate of vertigo treatment system was 63.08%,the effective rate of vertigo treatment system repositioning was higher than that of manual repositioning (P<0.05). The effective rate of manual repositioning in the low BMI group (58.70%) was higher than that in the high BMI group (14.75%), P<0.05. The effective rate of vertigo treatment system repositioning was higher in the low BMI group (75.00%) than that in the high BMI group (54.05%), but there was no statistically significant difference between the two BMI groups (P>0.05). Logistic regression analysis suggested that BMI was the influencing factor of HC-BPPV repositioning efficacy (P<0.05). Conclusion:BMI can affect the efficacy of HC-BPPV manual repositioning. Vertigo treatment system for HC-BPPV reduction is better than manual reduction, and the efficacy is not affected by BMI.


Assuntos
Vertigem Posicional Paroxística Benigna , Posicionamento do Paciente , Humanos , Vertigem Posicional Paroxística Benigna/terapia , Índice de Massa Corporal , Posicionamento do Paciente/métodos , Canais Semicirculares
13.
J Thorac Oncol ; 2022 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-36379356

RESUMO

Most published guidelines for genomic biomarker testing in non-small cell lung cancer (NSCLC) reflect the disease epidemiology and treatments readily available in Europe and North America. However, 60% of annual global NSCLC cases occur in Asia, where patient characteristics, tumor molecular profiles, and treatments vary greatly from the Western world. For example, mutations in the epidermal growth factor receptor (EGFR) occur at a higher prevalence in Asia than in other world regions. Although medical associations such as IASLC, ESMO, and ASCO have described principles for tumor genomic biomarker testing in NSCLC, there is a need for recommendations specific for Asia. This report provides consensus recommendations for NSCLC biomarker testing from Asian lung cancer experts, for clinicians working in Asia to improve patient care. These recommendations are divided into non-metastatic and metastatic forms of adenocarcinoma and squamous cell carcinoma. Biomarker testing approaches for actionable genetic alterations in EGFR, ALK, ROS1 and others are discussed. Owing to the higher prevalence of EGFR mutations in Asia, the experts emphasized the need for EGFR testing to include not just common mutations (exon 19 deletions & L858R substitutions) but also other uncommon EGFR mutations. In addition to the assessment of biomarkers in tumor tissue, the role of assessing tumor biomarkers by liquid biopsy are also discussed.

14.
J Virol ; : e0087922, 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36377874

RESUMO

The glycan loop of Zika virus (ZIKV) envelope protein (E) contains the glycosylation site and has been well documented to be important for viral pathogenesis and transmission. In the present study, we report that deletions in the E glycan loop, which were recorded in African ZIKV strains previously, have re-emerged in their contemporary Asian lineages. Here, we generated recombinant ZIKV containing specific deletions in the E glycan loop by reverse genetics. Extensive in vitro and in vivo characterization of these deletion mutants demonstrated an attenuated phenotype in an adult A129 mouse model and reduced oral infections in mosquitoes. Surprisingly, these glycan loop deletion mutants exhibited an enhanced neurovirulence phenotype, and resulted in a more severe microcephalic brain in neonatal mouse models. Crystal structures of the ZIKV E protein and a deletion mutant at 2.5 and 2.6 Å, respectively, revealed that deletion of the glycan loop induces encephalitic flavivirus-like conformational alterations, including the appearance of perforations on the surface and a clear change in the topology of the loops. Overall, our results demonstrate that the E glycan loop deletions represent neonatal mouse neurovirulence markers of ZIKV. IMPORTANCE Zika virus (ZIKV) has been identified as a cause of microcephaly and acquired evolutionary mutations since its discovery. Previously deletions in the E glycan loop were recorded in African ZIKV strains, which have re-emerged in the contemporary Asian lineages recently. The glycan loop deletion mutants are not glycosylated, which are attenuated in adult A129 mouse model and reduced oral infections in mosquitoes. More importantly, the glycan loop deletion mutants induce an encephalitic flavivirus-like conformational alteration in the E homodimer, resulting in a significant enhancement of neonatal mouse neurovirulence. This study underscores the critical role of glycan loop deletion mutants in ZIKV pathogenesis, highlighting a need for global virological surveillance for such ZIKV variants.

15.
Curr Microbiol ; 79(12): 381, 2022 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-36329290

RESUMO

A bacterium, designated 50T was isolated from the sediment of a pesticide plant in Shandong Province, PR China. The strain was non-motile, Gram stain-negative, rod shaped and grew optimally on NA medium at 30 °C, pH 7.5 and with 0% (w/v) NaCl. Strain 50T showed the highest 16S rRNA gene sequence similarity with Lysobacter pocheonensis Gsoil 193T (96.7%), followed by Luteimonas lumbrici 1.1416T (96.5%). Phylogenetic analyses based on 16S rRNA indicated that strain 50T and Luteimonas lumbrici 1.1416T were clustered with the genus of Lysobacter and formed a subclade with Lysobacter pocheonensis Gsoil 193T. In the phylogenetic analysis based on the genome sequences, strain 50T and Luteimonas lumbrici 1.1416T were also clustered with the type strains of the genus Lysobacter. The obtained ANI and the dDDH value between 50T and Luteimonas lumbrici 1.1416T were 80.6% and 24.0%, respectively. The respiratory quinone was ubiquinone-8 (Q-8), and the major cellular fatty acids were iso-C15: 0 (31.7%), summed feature 9 (iso-C17:1 ω9c or C16:0 10-methyl) (23.7%), iso-C17:0 (14.3%) and iso-C16:0 (12.6%). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and unidentified aminophospholipid, unidentified phospholipid and unidentified lipid. The genomic DNA G + C content was 69.5 mol%. According to the phenotypic, chemotaxonomic and phylogenetic analyses, strain 50T represents a novel species of the genus Lysobacter, for which the name Lysobacter sedimenti sp. nov. is proposed, with strain 50T (= KCTC 92088T = CCTCC AB 2022035T) as the type strain. In this study, it is also proposed that Luteimonas lumbrici should be transferred to the genus Lysobacter as Lysobacter lumbrici comb. nov. The type strain of Lysobacter lumbrici is 1.1416T (= KCTC 62979T = CCTCC AB 2018348T).


Assuntos
Lysobacter , Oligoquetos , Xanthomonadaceae , Animais , RNA Ribossômico 16S/genética , Filogenia , Oligoquetos/genética , Microbiologia do Solo , DNA Bacteriano/genética , Técnicas de Tipagem Bacteriana , Análise de Sequência de DNA , Xanthomonadaceae/genética , Fosfolipídeos/química , Ácidos Graxos/química
16.
ACS Omega ; 7(42): 37452-37464, 2022 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-36312426

RESUMO

Manganese has excellent performance in removing metal ions from aqueous solutions, but there are few studies on the adsorption and removal of heavy metal impurities in metal salt solutions. In this paper, the adsorption of cobalt and nickel ions in MnSO4 solution by δ-MnO2 prepared from two different manganese sources was studied. The optimum adsorption conditions were as follows: When the concentration of Mn2+ was 20 g/L, δ-MnO2 addition was 10 g/L, Co2+ concentration was 80 mg/L, Ni2+ concentration was 80 mg/L, reaction time was 60 min, reaction temperature was 80 °C, and pH value was 7, the adsorption rate of Co2+ and Ni2+ reached more than 80%. The manganese dioxide adsorbed by heavy metals was analyzed and detected. The results showed that MnOOH appeared in the phases of both kinds of δ-MnO2, and their morphologies were dense rod-like structures with different lengths and flake-like structures of fine particles. Co and Ni were distributed on the surface and gap of MnO2 particles, and the atomic percentage of Co was slightly higher than that of Ni. The new vibration peaks appeared near wave numbers of 2668.32, 1401.00, and 2052.19 cm-1, which were caused by the complexation of cations such as Co2 + and Ni2 + with hydroxyl groups. Some cobalt and nickel appeared on the surface of δ-MnO2, and the surface oxygen increased after adsorption. The above characterization revealed that the adsorption of cobalt and nickel in manganese sulfate by δ-MnO2 was realized by the reaction of its surface hydroxyl with metal ions (M) to form ≡SOMOH.

17.
Front Oncol ; 12: 917897, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36248974

RESUMO

Pancreatic cancer is the 7th leading cause of cancer death worldwide, and its incidence and mortality rate have been on the rise in recent years in Western developed countries. The specificity of the disease and the lack of appropriate treatments have resulted in a 5-year overall survival rate of only 9%. In this study, we conducted a study based on the TCGA database and GEO database and analyzed using the energy metabolism gene set to establish a prognostic model with the least absolute shrinkage and selection operator to identify 7-genes prognostic signature, and the gene expression was verified by Real-time PCR. The model was validated using a risk score calculation, and the OS rates of the 7 genes were analyzed using one-way Cox regression. The prognostic relationship between vesicle-associated membrane protein 2 (VAMP2) and pancreatic cancer patients was analyzed by OS and progression-free survival, and the prognosis was found to be significantly worse in the high-expression group. A Nomogram showed that VAMP2 was an independent prognostic factor in pancreatic cancer. Gene set enrichment analysis showed that VAMP2 upregulation was enriched in pathways associated with immune response and that VAMP2 downregulation was enriched in metabolism-related pathways. The association of VAMP2 with immune cell infiltration was analyzed for the enrichment results, and VAMP2 was found to be positively associated with all 6 immune cells. The results of this study suggest that VAMP2 is an independent prognostic factor associated with energy metabolism in pancreatic cancer and may be involved in the immune response.

18.
BMC Plant Biol ; 22(1): 493, 2022 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-36271339

RESUMO

BACKGROUND: Numerous studies have shown that gluten aggregation properties directly affect the processing quality of wheat, however, the genetic basis of gluten aggregation properties were rarely reported. RESULTS: To explore the genetic basis of gluten aggregation properties in wheat, an association population consisted with 207 wheat genotypes were constructed for evaluating nine parameters of aggregation properties on GlutoPeak across three-year planting seasons. A total of 940 significant SNPs were detected for 9 GlutoPeak parameters through genome-wide association analysis (GWAS). Finally, these SNPs were integrated to 68 non-redundant QTL distributed on 20 chromosomes and 54 QTL was assigned as pleiotropic loci which accounting for multiple parameters of gluten aggregation property. Furthermore, the peak SNPs representing 54 QTL domonstrated additive effect on all the traits. There was a significant positive correlation between the number of favorable alleles and the phenotypic values of each parameter. Peak SNPs of two novel QTL, q3AL.2 and q4DL, which contributing to both PMT (peak maximum time) and A3 (area from the first minimum to torque 15 s before the maximum torque) parameters, were selected for KASP (Kompetitive Allele Specific PCR) markers development and the KASP markers can be used for effectively evaluating the quality of gluten aggregation properties in the association population. CONCLUSION: The rapid and efficient GlutoPeak method for gluten measurement can be used for early selection of wheat breeding. This study revealed the genetic loci related to GlutoPeak parameters in association population, which would be helpful to develop wheat elite lines with improved gluten aggregation through molecular marker-assisted breeding.


Assuntos
Estudo de Associação Genômica Ampla , Triticum , Triticum/genética , Locos de Características Quantitativas/genética , Mapeamento Cromossômico , Glutens/genética , Melhoramento Vegetal , Polimorfismo de Nucleotídeo Único , Fenótipo
19.
EMBO Mol Med ; 14(11): e16818, 2022 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-36256519

RESUMO

There is an unmet clinical need to end the COVID-19 pandemic. In the past 2 years, the SARS-CoV-2 continued to evolve and poses a critical challenge to the efficacy of the vaccine and neutralizing antibody therapies. The fifth wave of the pandemic is driven by the Omicron variants, due to their ability to evade prior immunity and their resistance to therapeutic antibodies. The report by Zhang et al in the current issue of EMBO Molecular Medicine shows that the engineered decoy ACE2 can reduce lung injury and improve survival in K18-hACE2 transgenic mice inoculated with a lethal dose of the SARS-CoV-2 and potentially targets the Omicron variant.


Assuntos
COVID-19 , SARS-CoV-2 , Camundongos , Animais , Humanos , COVID-19/terapia , Glicoproteína da Espícula de Coronavírus/genética , Pandemias , Anticorpos Neutralizantes/uso terapêutico , Camundongos Transgênicos , Anticorpos Antivirais
20.
Front Microbiol ; 13: 1016996, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36212850

RESUMO

Early blight (EB) disease, caused mainly by Alternaria solani, is an economic threat to potato and tomato production worldwide. Thus, accurate and sensitive detection of the fungal pathogen of this disease in plants at the early infection stage is important for forecasting EB epidemics. In this study, we developed an RNA-based method that enables highly accurate and sensitive A. solani detection in a whole potato leaf at a single spore level based on quantitative real-time polymerase chain reaction (qPCR). We discovered jg1677, a highly expressed gene whose full-length coding sequence is very specific for A. solani, by analyzing A. solani transcripts isolated from enhanced high throughput transcriptome of infected potato leaves by A. solani and using the National Center for Biotechnology Information's basic local alignment search tool. The specificity of the primers derived from jg1677 was determined using 22 isolates of common potato pathogens, including seven Alternaria isolates. Detecting jg1677 transcripts with qPCR is 1,295 times more sensitive than detecting genomic DNA. In addition, the expression pattern of jg1677 at different infection stages was determined by qPCR. What is more, jg1677 was expressed relatively stable between 15 and 35°C in infected leaves, and its expression was virtually unaffected in isolated leaves left at room temperature for 24 h. Our work provides a much more sensitive and accurate method compared to conditional DNA-based ones, permitting a very early diagnosis of EB and lowering the risk of EB epidemics.

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