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1.
Nanotechnology ; 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31791018

RESUMO

A great deal of engineering effort is focused on developing stretchable strain sensors for human motion monitoring and wearable devices. The ultrasensitivity and fast response under tiny strain (1%) while maintaining the working range remain the grand challenge. In this work, we propose an entirely stretchable strain sensor based on the sandwich sensing film, which is fabricated by vacuum filtration of silver nanowires (AgNWs)/ graphene/ AgNWs in sequence and the injection of liquid metal as electrodes. The novel sandwich sensing film endows the stretchable strain sensor high sensitivity under tiny strain (Gauge factor=111.5 at 1%), fast response (<10ms), relative large working range (0∽35%) with a maximum gauge factor of 1403.7, followed by good linearity, long-term durability, and the recovery property from being overstretched (>100\%). The excellent performance is due to the slippage of the inner graphene under tiny strain, whereas the 'sewing' phenomenon of the outer AgNWs under larger strain. The sandwich structure illustrates a better combination of graphene and AgNWs than other hybrid methods, showing great potential in wearable devices and soft robotics.

2.
Artigo em Inglês | MEDLINE | ID: mdl-31802603

RESUMO

The design and scalable synthesis of robust 2D biological ultrathin films with a tunable structure and function and the ability to be easily transferred to a virtually arbitrary substrate remain key challenges in chemistry and materials science. Here, we implicate the potential of the thiol-disulfide exchange reaction in the synthesis of a macroscopic 2D ultrathin proteinaceous film with the potential for scaled-up fabrication and on-demand encapsulation/release of functional molecules. The synthesis success is determined by the chemoselectivity and site-specificity of the reaction between the Cys6-Cys127 disulfide bond of native lysozyme and cysteine, as supported by the superior redox potential of the Cys6-Cys127 disulfide bond over that of other disulfide bonds in native lysozyme and other unreactive proteins. The partially unfolded lysozyme-cysteine conjugate monomers are formed by this strategy, which then further aggregate at the air/water or solid/liquid interface to form an ultralarge 2D nanofilm (e.g., 900 cm 2 ) with ~100% optical transparency. On the basis of amyloid-like aggregation inside the nanofilm, this material presents robust adhesion onto a virtually arbitrary substrate and on-demand controllability to mediate the encapsulation and release of small, mid-sized and large molecules without significant activity loss upon the surface immobilization of active proteins. Our approach provides an important application for the thiol-disulfide exchange reaction in the biocompatible large-scale preparation of 2D proteinaceous nanofilms and coatings with the capability of tunable functional molecular block encapsulation and release.

3.
Clin Lab ; 65(11)2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31710430

RESUMO

BACKGROUND: Spontaneous subarachnoid hemorrhage (SSAH) and nontraumatic acute headache patients presented with symptoms similar to headache. This study was to investigate the diagnostic value of D-dimer (DD) in distinguishing patients with SSAH from those with nontraumatic acute headache. METHODS: This retrospective study was performed and data were gathered from medical records of patients with acute headache symptoms from the Emergency Department and Neurology Clinics who were admitted to the Fourth Affiliated Hospital Zhejiang University School of Medicine between January 2015 and January 2019. Of the total 114 patients with headache symptoms enrolled in this study, 46 patients were diagnosed with SSAH (SAH group) and 68 cases with nontraumatic acute headache due to other causes (non-SAH group). The plasmatic levels of PT, INR, APTT, TT, FIB, and DD were compared between the two groups. Student's t-test of independent samples was adopted for comparing the mean between the two groups. Model discrimination was evaluated using the area under the receiver operating characteristic curve (AUC). Comparison of AUC was performed using the Z-test. RESULTS: There were no significant statistical differences of the PT, INR, TT and FIB (all p > 0.05). The APTT was significantly decreased in the SSAH group compared with non-SAH group, while DD was significantly increased (all p < 0.001); moreover, AUC in distinguishing patients with SSAH from those with nontraumatic acute headache was 0.721 (95% confidence interval (CI), 0.629 to 0.801) for APTT and 0.886 (95% CI, 0.813 to 0.938) for DD. There was a significant statistical difference (p < 0.01). Finally, the cutoff values were 25.2s in distinguishing patients with SSAH from those with nontraumatic acute headache (specificity 73.53% and sensitivity 60.87%) for APTT and 0.31 mg/L (specificity 83.82% and sensitivity 84.78%) for DD. CONCLUSIONS: As an easy-to-obtain and potential biomarker, DD could demonstrate more accurate and reliable diagnostic value than APTT in distinguishing between SSAH and nontraumatic acute headache.

4.
Jpn J Infect Dis ; 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31666495

RESUMO

Estimation of prevalence and genotypes distribution of High-risk Human Papillomavirus (HR-HPV) is important for formulating strategies for prevention and screening of cervical cancer. This study aimed to determine HR-HPV prevalence and genotypes distribution among Chinese women living in Sichuan province. A total of 3089 women were recruited from the Third people's,Hospital of Chengdu in Sichuan province. HR-HPV genotyping were assessed by real-time quantitative PCR. Up to 720 cases (23.3%) were HR-HPV positive. 81.1% (584/720) were detected for a single HR-HPV genotype and 18.9% (136/720) for multiple genotypes. The most frequently detected HPV genotype was HPV52, followed by HPV58, HPV16, and HPV51. Age subgroup analysis showed two peaks for the frequencies of HPV infections, one for the group of women under 29 years old, and the other group over 50 years old. Among the women for whom cytology results were available, HPV16 was the most common detected genotype among women affected by HSIL (32.5%) and SCC, followed by HPV58, HPV33 and HPV52.This is one of the largest studies to demonstrate HPV prevalence and genotypes distribution among Chinese women in Sichuan. The prevalence and genotype distribution of HR-HPV was unique with higher frequencies of HPV52 and HPV58.

5.
Pathol Oncol Res ; 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31758409

RESUMO

The aim of this study was to characterize secondary kinase mutations in Chinese patients with imatinib-resistant gastrointestinal stromal tumors (GISTs). Mutations in receptor tyrosine kinase (KIT; exons 9, 11, 13, 14, 17, and 18) and platelet-derived growth factor-alpha (PDGFRA; exons 12, 14, and 18) were analyzed by direct sequencing. After imatinib treatment, 425 cancer-related target genes were analyzed by next generation sequencing (NGS) in imatinib-resistant patients. Correlation of sequencing results with clinicopathologic features were analyzed. We identified 320 patients with secondary acquired resistance. We determined that 65.63% (210/320) of resistant patients had secondary KIT mutations in exon 13 (n = 134), exon 14 (n = 10), or exon 17 (n = 66), and 4.38% (14/320) had additional PDGFRA mutations in exon 14 (n = 3) or exon 18 (n = 11). All secondary KIT mutations were missense mutations and were mostly located in kinase domains. Ninety-six imatinib-resistant GIST patients did not have secondary KIT or PDGFRA mutations. Common independent mutation events were found in retinoblastoma protein 1 (RB1) (18/96 cases), SWI/SNF-related matrix associated actin-dependent regulator of chromatin subfamily B member 1 (SMARCB1) (16/96 cases), and myc-associated factor X (MAX) (10/96 cases). RB1 or SMARCB1 mutations coexisted with activation of other oncogenes in 6 or 15 cases, respectively. Multiple mutations were also seen in cases with MAX mutations. These mutations are frequently associated with clinicopathological factors. Secondary mutations of KIT/PDGFRA were the most important contributors in GISTs developing resistance to imatinib treatment. Additional genetic events including RB1, SMARCB1, and MAX except secondary KIT/PDGFRA mutations are the most common for GISTs to evolve into resistant disease. Clinical assessment of the effect of these mutations may benefit existing risk assessment models and selection of adjuvant therapies in GIST patients.

6.
Int J Surg ; 72: 206-213, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31734255

RESUMO

BACKGROUND: Long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (lncRNA, MALAT1) has been found to be aberrantly expressed in osteosarcoma, while high MALAT1 expression is correlated with both metastasis and prognosis. This meta-analysis set out to investigate the prognostic value of lncRNA MALAT1 in patients living with osteosarcoma. METHODS: We conducted a systematical search of available databases from inception to May 2019. Odds ratios (OR) of clinical parameters, as well as hazard ratio (HR) of overall survival (OS), were calculated in order to evaluate the relationship between MALAT1 expression and the prognosis of patients living with osteosarcoma. RESULTS: Nine eligible studies which included a total of 599 osteosarcoma patients were enrolled in the present study. Pooled results found that high MALAT1 expression was associated with clinical stage and distant metastasis, but not age, gender, tumor anatomical location or tumor size. When compared to patients with low MALAT1 expression, patients with high MALAT1 expression were markedly correlated with a worse OS. Moreover, MALAT1 may be an independent predictive factor for OS in patients living with osteosarcoma. CONCLUSIONS: This meta-analysis suggests that high MALAT1 expression is associated with advanced clinicopathological features as well as unfavorable prognosis. LncRNA MALAT1 has the potential to serve as a moderate prognostic biomarker for osteosarcoma.

7.
Inorg Chem ; 58(23): 15864-15871, 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31725279

RESUMO

We have prepared the indium(III)-centered, all-acetate-capped polyoxopalladate(II) nanocube [InPd12O8(OAc)16]5- (InPd12Ac16), which can be further used as precursor to form the phosphate-capped (i) double-cube [In2Pd23O17(OH)(PO4)12(PO3OH)]21- (In2Pd23P13) and (ii) monocube [InPd12O8(PO4)8]13- (InPd12P8). All three novel polyoxopalladates (POPs) were synthesized using conventional one-pot techniques in aqueous solution and characterized in the solid state (single-crystal XRD, IR, elemental analysis), in solution (115In, 31P, and 13C NMR), and in the gas phase (ESI-MS).

8.
Artigo em Inglês | MEDLINE | ID: mdl-31783428

RESUMO

BACKGROUND AND AIM: Nowadays, anti-inflammation treatment is a promising approach for preventing tumorigenesis, and human microflora is closely related to inflammation. This study aimed to investigate the gastric cardiac microbiome and identify inflammation-related microorganisms for gastric cardiac inflammation. METHODS: We performed 16S rRNA sequencing on a total of 11 healthy and 89 individuals with different degree of gastric cardiac inflammation. Immunohistochemistry was used for verifying candidate bacteria. Phylogenetic reconstruction of unobserved states (PICRUSTs) was used for predicting the pathways involved by cardiac microflora. RESULTS: The resident phyla in normal were Proteobacteria, Firmicutes, Bacteroides and Actinobacteria and the dominant genus in normal were Halomonas, shewanella and Comamonas. In the progression of gastric cardiac inflammation, the diversity of cardiac microflora did not change(P > 0.05). However, the composition structure of cardiac microflora varied between healthy and inflamed tissues (P < 0.05). Meanwhile, there were 64 species parallel increased with inflammation degree, especially H. pylori, Lactobacillus Spp.. Additionally, inflammation-related species were detected (P < 0.05), including H. pylori, Acinetobacter ursingii, Streptococcus agalactiae. Higher H. pylori colonization was positively related to the progression of cardiac inflammation (gamma coefficient = 0.678, P< 0.001), and it also influenced the cardiac microbial community structure. Cardiac microflora also participated in DNA repair pathways, and affected by the relative abundance of H. pylori(P < 0.0001). CONCLUSIONS: Cardiac microflora dysbiosis promoted the progression of cardiac inflammation, especially H. pylori, providing.

9.
Anal Chem ; 91(23): 14920-14926, 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31674756

RESUMO

Herein, a novel three-dimensional (3D) DNA nanomachine with high walking efficiency via free DNA walker movement on biomimetic lipid bilayers supported by hard silica@CdTe quantum dots (SiO2@CdTe) was constructed for ultrasensitive fluorescence detection of microRNA. The synthesized SiO2@CdTe nanoparticles were adopted as the fluorescence indicator and spherical carrier of lipid bilayers, and then the DNA substrates were anchored on lipid bilayers with biomimetic fluidity through the cholesterol-lipid interaction. Once target microRNA-141 interacted with the 3D DNA nanomachine to release cholesterol labeled arm (Chol-arm), the Chol-arm could generate a series of strand displacement reactions by moving freely on the lipid bilayers, resulting in the releasement of numerous quenchers from the SiO2@CdTe nanoparticles and inducing a strong fluorescence signal. Impressively, compared with traditional 3D DNA nanomachine conjugating DNA substrates on hard surfaces (such as gold or silica) with limited reactivity, the proposed biomimetic 3D DNA nanomachine not only immobilized DNA substrates rapidly and effectively but also kept it with a favorable fluidity, which significantly enhanced the walking efficiency. As expected, the biomimetic 3D DNA nanomachine for fluorescence detection of microRNA-141 exhibited an excellent performance with a detection limit of 0.21 pM and presented promising properties in cell lysate detection and intracellular imaging. Thus, the described biomimetic 3D DNA nanomachine provided a novel avenue for sensitive detection of biomolecules, which could be useful for bioanalysis and early clinical diagnoses of disease.

10.
Chem Biodivers ; 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31705573

RESUMO

A facile method was developed for synthesis of boronic acid-functionalized silica nanocomposites (SiO 2 -BA) by "thiol-ene"click reaction, where silica nanoparticles were synthesized by using tetraethoxysilane (TEOS) and γ-mercaptopropyl trimethoxysilane (γ-MPTS) as precursors. The morphology and structure properties of the resultant SiO 2 -BA were characterized by Transmission electronic microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR),and Brunner-Emmet-Teller measurements  (BET). The adsorption behavior of the SiO 2 -BA for glycoproteins was evaluated. Under the optimized conditions, the SiO 2 -BA exhibited higher adsorption capacity towards glycoproteins (ovalbumin, OVA, (7.64 µmol/g) than non-glycoproteins (bovine serum albumin,BSA, 0.83 µmol/g). In addition, the practicality of the SiO 2 -BA was further assessed by selective enrichment of glycophoproteins from egg white samples.

11.
Sci Total Environ ; 703: 135005, 2019 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-31733497

RESUMO

Land-use and land-cover changes associated with urbanization have significantly influenced biodiversity and ecosystem functions, as well as the supply and demand of ecosystem services (ESs). Assessing ESs and exploring their drivers are critical for regional land-use planning and ecological sustainability. In this study, the supply-demand matrix approach was used to quantify ES supply, demand, and their gap at multiple scales across the conterminous United States from 1940 to 2011. A new integrated measurement framework was proposed to offset ES deficits by identifying an optimal land-use conversion strategy. We focused on exploring the scale and spatial effects of the impacts of various drivers on ESs using ordination and regression analysis. The results showed that the expansion of developed land led to decreased ES supply and increased ES demand during the past seven decades, generating growing ES deficits at different scales, especially in highly urbanized metropolitan areas. To alleviate or offset ES deficits, promoting the intensive utilization of developed land and converting cropland, pasture, and barren land into forests would be the optimal land use strategies. Moreover, the drivers of ESs exhibited not only scale dependence but also spatial heterogeneity. The smaller the scale, the more diverse the drivers. The natural and socioeconomic drivers explained less variation at the metropolitan scale than at the state scale. Economic factors were key drivers for ESs at the state scale, while social factors were key drivers at the metropolitan scale. The regression coefficients for the drivers of ESs in the geographically weighted regression (GWR) model showed remarkable spatial heterogeneity. The GWR coefficients might have important implications for decision making in ES management. Localized and efficient land-use strategies and management policies are needed to reduce the ecological footprints of urban areas and thus achieve regional sustainability.

12.
Dalton Trans ; 2019 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-31773120

RESUMO

Covalent organic frameworks (COFs) have been utilized as molecular sieves to adsorb or remove or separate a wide range of substances. Herein, a new carboxyl-containing COF (COF-COOH) is fabricated from the polymerization of 1,3,5-triformylphloroglucinol (TP) and 4,4'-diamino-[1,1'-biphenyl]-3,3'-dicarboxylic acid (DBA). COF-COOH displays good adsorption performance on Congo red (CR) through hydrogen bonds and π-π stacking interactions. Through post-modification with Ca2+/Ni2+ ions, the adsorption capacity of COF-COOCa/COF-COONi to CR is improved, which is due to the coordinated metal cations having a positive contribution to the electrostatic interactions. At 25 °C, the maximum adsorption amount of COF-COOCa and COF-COONi to CR is 704.23 mg g-1 and 781.25 mg g-1, respectively. The removal efficiency of COF-COOCa to CR is 95% and that of COF-COONi is 96%. This demonstrates that the new metal ion-assisted COFs are viable adsorbents to remove dye pollutants, which are harmful to the environment and to human health, from wastewater.

13.
Nat Commun ; 10(1): 5338, 2019 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-31767845

RESUMO

Many platinum group metal-free inorganic catalysts have demonstrated high intrinsic activity for diverse important electrode reactions, but their practical use often suffers from undesirable structural degradation and hence poor stability, especially in acidic media. We report here an alkali-heating synthesis to achieve phase-mixed cobalt diselenide material with nearly homogeneous distribution of cubic and orthorhombic phases. Using water electroreduction as a model reaction, we observe that the phase-mixed cobalt diselenide reaches the current density of 10 milliamperes per square centimeter at overpotential of mere 124 millivolts in acidic electrolyte. The catalyst shows no sign of deactivation after more than 400 h of continuous operation and the polarization curve is well retained after 50,000 potential cycles. Experimental and computational investigations uncover a boosted covalency between Co and Se atoms resulting from the phase mixture, which substantially enhances the lattice robustness and thereby the material stability. The findings provide promising design strategy for long-lived catalysts in acid through crystal phase engineering.

14.
J Am Chem Soc ; 2019 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-31707779

RESUMO

Rubriflordilactone B (1) is a schinortriterpenoid isolated by Sun and colleagues, which possesses a tetrasubstituted benzene moiety and eight stereocenters. The previous synthesis of 1 by Li and co-workers uncovered the existence of its naturally occurring stereoisomer "pseudorubriflordilactone B". Here we report a collaborative study by the two groups that elucidates the structure of pseudorubriflordilactone B to be 16,17-bis-epi-rubriflordilactone B (26). Chemical synthesis served as an important tool in the structure determination. Taking advantage of a modular synthetic route, we systematically "mutated" the configurations of C-23, C-22, C-20, and C-16/C-17 located at the right-hand domain of 1, and thus prepared its fifteen stereoisomers for spectrum comparison. The 1H NMR spectra of synthetic 26 in deuterated chloroform and pyridine were identical to those of authentic pseudorubriflordilactone B, respectively. This synthetic sample displayed anti-HIV activity (EC50 = 0.288 µM) in vitro.

15.
ACS Appl Mater Interfaces ; 11(45): 42671-42679, 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31663328

RESUMO

Melanin and its synthetic analogs (i.e., polydopamine nanomaterials) are able to transform a near-infrared (NIR) light energy source to heat for the selective killing of cancer cells. Although many of the effects on these nontoxic photothermal agents have been well documented, a concern has arisen that the extended usage of these natural and synthetic melanins might be hindered by their limited photothermal effects under low-density light irradiation. To address this issue, herein, we propose a rational and green fabrication strategy toward a new class of synthetic melanin nanoparticles (SMNPs) with superior photothermal effects via the one-pot copolymerization of two kinds of naturally occurring monomers (arginine and dopamine). The total photothermal efficiencies of these arginine-doped SMNPs could be significantly improved (i.e., ∼60% increase) by enhancing 808 nm NIR light absorption via the construction of donor-acceptor microstructures within SMNPs and decreasing nonthermal radiative transition processes via the increase of free radical concentrations within SMNPs. The resulting SMNPs demonstrated higher photothermal therapy efficiencies in both killing 4T1 cancer cells in vitro and suppressing tumor growth and recurrence compared with conventional agents. This work offers new opportunities in the structural and functional tailoring of melanin-inspired nanomaterials for cancer treatment via green fabrication strategies.

16.
IEEE Trans Cybern ; 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31567112

RESUMO

Alzheimer's disease (AD) is a neurodegenerative disease with an irreversible and progressive process. To understand the brain functions and identify the biomarkers of AD and early stages of the disease [also known as, mild cognitive impairment (MCI)], it is crucial to build the brain functional connectivity network (BFCN) using resting-state functional magnetic resonance imaging (rs-fMRI). Existing methods have been mainly developed using only a single time-point rs-fMRI data for classification. In fact, multiple time-point data is more effective than a single time-point data in diagnosing brain diseases by monitoring the disease progression patterns using longitudinal analysis. In this article, we utilize multiple rs-fMRI time-point to identify early MCI (EMCI) and late MCI (LMCI), by integrating the fused sparse network (FSN) model with parameter-free centralized (PFC) learning. Specifically, we first construct the FSN framework by building multiple time-point BFCNs. The multitask learning via PFC is then leveraged for longitudinal analysis of EMCI and LMCI. Accordingly, we can jointly learn the multiple time-point features constructed from the BFCN model. The proposed PFC method can automatically balance the contributions of different time-point information via learned specific and common features. Finally, the selected multiple time-point features are fused by a similarity network fusion (SNF) method. Our proposed method is evaluated on the public AD neuroimaging initiative phase-2 (ADNI-2) database. The experimental results demonstrate that our method can achieve quite promising performance and outperform the state-of-the-art methods.

17.
Int J Gynecol Pathol ; 2019 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-31569185

RESUMO

Endometrial stromal sarcoma (ESS) is a rare malignant tumor of the uterus that has been described as the second most common malignant uterine mesenchymal tumor. The Primary extrauterine ESS (EESS) is an extremely uncommon occurrence. We hereby report a new bona fide case of low-grade EESS in a 74-yr-old woman arising in the vagina, presenting as a polypoid mass associated with irregular vaginal bleeding. On examination, a 6×2×2 cm polypoid mass was found in the left vaginal wall. Consequently, the patient underwent partial vaginectomy and repair. No ESS or endometriotic lesion was found in the endometrium and bilateral adnexa. The diagnosis of ESS performed by typical pathologic and immunohistochemical evaluation was as follows: beta-catenin (+++), estrogen receptor (+++), progesterone receptor (++), vimentin (++), and uniformly negative for CD10, EMA, CD31, CD34, CD117,CD99, SMA, desmin, h-caldesmon, S-100, MelanA, and HMB45. She has remained disease free with no signs or symptoms of recurrent or advanced disease for 46 mo. Although CD10 is the most useful immunohistochemical marker for the diagnosis of this tumor, negative CD10 staining can be encountered with underfixation. Therefore, it is important to use a panel of immunostains that includes CD10, beta-catenin, and smooth muscle markers. The present study describes the clinical and pathologic features of low-grade EESS through a case report and literature review. To the best of our knowledge, this is the eighth report of EESS arising from the vagina.

18.
Nat Chem Biol ; 15(12): 1214-1222, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31591566

RESUMO

Iron is essential for a broad range of biochemical processes in the brain, but the mechanisms of iron metabolism in the brain remain elusive. Here we show that iron functionally translocates among brain regions along specific axonal projections. We identified two pathways for iron transport in the brain: a pathway from ventral hippocampus (vHip) to medial prefrontal cortex (mPFC) to substantia nigra; and a pathway from thalamus (Tha) to amygdala (AMG) to mPFC. While vHip-mPFC transport modulates anxiety-related behaviors, impairment of Tha-AMG-mPFC transport did not. Moreover, vHip-mPFC iron transport is necessary for the behavioral effects of diazepam, a well-known anxiolytic drug. By contrast, genetic or pharmacological promotion of vHip-mPFC transport produced anxiolytic-like effects and restored anxiety-like behaviors induced by repeated restraint stress. Taken together, these findings provide key insights into iron metabolism in the brain and identify the mechanisms underlying iron transport in the brain as a potential target for development of novel anxiety treatments.

19.
Hum Vaccin Immunother ; : 1-7, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31596661

RESUMO

Background: Since 2007, trivalent inactivated influenza vaccine (TIV) has been provided free-of-charge to primary, middle school and high school students in Beijing. However, there have been few school-based studies on influenza vaccine effectiveness (VE). In this report, we estimated influenza VE against laboratory-confirmed influenza illness among school children in Beijing, China during the 2016-2017 influenza season.Methods: The VE of 2016-2017 TIV against laboratory-confirmed influenza virus infection among school-age children was assessed through a case-control design. Conditional logistic regression was conducted on matched case-control sets to estimate VE. The effect of prior vaccination on current VE was also examined.Results: All 176 samples tested positive for influenza A virus with the positive rate of 55.5%. The average coverage rate of 2016-2017 TIV among students across the 37 schools was 30.6%. The fully adjusted VE of 2016-2017 TIV against laboratory-confirmed influenza was 69% (95% CI: 51 to 81): 60% (95% CI: -15 to 86) for influenza A(H1N1)pdm09 and 73% (95% CI: 52 to 84) for influenza A(H3N2). The overall VE for receipt of 2015-2016 vaccination only, 2016-2017 vaccination only, and vaccinations in both seasons was 46% (95% CI: -5 to 72), 77% (95% CI: 58 to 87), and 57% (95%CI: 17 to 78), respectively.Conclusions: Our study during school outbreaks found that VE of 2016-2017 TIV was moderate against influenza A(H3N2) as well as A(H1N1)pdm09 viruses.

20.
Ecotoxicol Environ Saf ; 186: 109772, 2019 Dec 30.
Artigo em Inglês | MEDLINE | ID: mdl-31614297

RESUMO

Trichloroethylene (TCE) is a common organic solvent which can cause TCE hypersensitivity syndrome (THS) in exposure workers. THS is an adverse skin disorder with severe inflammatory kidney damage. Complement C3a receptor (C3aR) acts as a specific receptor for the key complement cleavage product C3a and involves multiple inflammatory responses, but the role of C3aR in TCE induced kidney inflammatory injury remains unknown. In this study, BALB/c mouse model of skin sensitization induced by TCE was set up in the presence or absence of C3aR antagonist (C3aRA). Kidney pathology and renal function, expression of inflammatory mediators and C3aR, changes in Th17 cell numbers, and activation of signal transducer and activator of transcription 3 (STAT3) in the kidney were examined. TCE sensitization produced histopathological and functional damage to the kidney, accompanied by increased levels of interleukin (IL-) 1ß, IL-6, and IL-23. Local accumulation of Th17 cells and enhanced phosphorylation of STAT3 were also seen in the impaired kidney in TCE sensitization-positive mice. C3aR was mainly located in the impaired glomerulus and upregulated in TCE sensitization-positive mice. C3aRA pretreatment alleviated the structural and functional kidney damage and the inflammatory cytokine and Th17 responses by TCE sensitization, and specifically reduced the phosphorylation of STAT3. Together, our results demonstrate that C3aR signaling promotes the inflammatory responses and regulates the accumulation of Th17 phenotype via phosphorylation of STAT3 in TCE sensitization induced inflammatory kidney damage. C3aR may serve as a potential therapeutic target in TCE sensitization mediated kidney injury.

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