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1.
J Hazard Mater ; 382: 121058, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31450213

RESUMO

In present work, the degradation of antibiotic and inactivation of antibiotic resistance genes (ARGs) in cephalosporin C fermentation (CEPF) residues were performed using ionizing radiation, ozonation and thermal treatment. The results showed that the three treatment methods could degrade cephalosporin C effectively, with the removal efficiency of 85.5% for radiation at dose of 100 kGy, 79.9% for ozonation at dosage of 5.2 g O3/L, and 71.9% and 87.3% for thermal treatment at 60 °C and 90 °C for 4 h. The cephalosporin resistance gene tolC was detected in the raw CEPF residues, and its abundance was decrease 74.2% by radiation, 64.6% by ozonation and 26.9%-37.1% by thermal treatment respectively. The presence of protein, glucose and acetate in the CEPF residues had inhibitive influence on the degradation of cephalosporin C by ionizing radiation, and the effect was more significant when the antibiotic concentration was lower. The total content of COD, polysaccharides and protein changed slightly after radiation and thermal treatment, while they were decreased greatly by ozonation. The primary techno-economic analysis showed that the operational cost of ionizing radiation by electron beam at 50 kGy ($5.2/m3) was comparable to thermal treatment ($4.3-7.9/m3), which was more economical than ozonation ($14.6/m3).

2.
Ophthalmology ; 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31585827

RESUMO

PURPOSE: To describe and compare associations with macular retinal nerve fiber layer (mRNFL), ganglion cell complex (GCC), and ganglion cell-inner plexiform layer (GCIPL) thicknesses in a large cohort. DESIGN: Cross-sectional study. PARTICIPANTS: We included 42 044 participants in the UK Biobank. The mean age was 56 years. METHODS: Spectral-domain OCT macular images were segmented and analyzed. Corneal-compensated intraocular pressure (IOPcc) was measured with the Ocular Response Analyzer (Reichert, Corp., Buffalo, NY). Multivariable linear regression was used to examine associations with mean mRNFL, GCC, and GCIPL thicknesses. Factors examined were age, sex, ethnicity, height, body mass index (BMI), smoking status, alcohol intake, Townsend deprivation index, education level, diabetes status, spherical equivalent, and IOPcc. MAIN OUTCOME MEASURES: Thicknesses of mRNFL, GCC, and GCIPL. RESULTS: We identified several novel independent associations with thinner inner retinal thickness. Thinner inner retina was associated with alcohol intake (most significant for GCIPL: -0.46 µm for daily or almost daily intake compared with special occasion only or never [95% confidence interval (CI), 0.61-0.30]; P = 1.1×10-8), greater social deprivation (most significant for GCIPL: -0.28 µm for most deprived quartile compared with least deprived quartile [95% CI, -0.42 to -0.14]; P = 6.6×10-5), lower educational attainment (most significant for mRNFL: -0.36 µm for less than O level compared with degree level [95% CI, -0.45 to 0.26]; P = 2.3×10-14), and nonwhite ethnicity (most significant for mRNFL comparing blacks with whites: -1.65 µm [95% CI, -1.86 to -1.43]; P = 2.4×10-50). Corneal-compensated intraocular pressure was associated most significantly with GCIPL (-0.04 µm/mmHg [95% CI, -0.05 to -0.03]; P = 4.0×10-10) and was not associated significantly with mRNFL (0.00 µm/mmHg [95% CI, -0.01 to 0.01]; P = 0.77). The variables examined explained a greater proportion of the variance of GCIPL (11%) than GCC (6%) or mRNFL (7%). CONCLUSIONS: The novel associations we identified may be important to consider when using inner retinal parameters as a diagnostic tool. Associations generally were strongest with GCIPL, particularly for IOP. This suggests that GCIPL may be the superior inner retinal biomarker for macular pathophysiologic processes and especially for glaucoma.

3.
Artigo em Inglês | MEDLINE | ID: mdl-31606425

RESUMO

OBJECTIVE: This study aimed to explore the relationship between the expression of the coxsackie-adenovirus receptor (CAR) in oral squamous cell carcinoma (OSCC) and the clinicopathologic parameters associated with the disease. The diagnostic and prognostic potential of CAR in OSCC was also investigated. STUDY DESIGN: Immunohistochemistry was performed on human tissue microarrays, containing 42 oral mucosa, 69 dysplasia, and 176 OSCC tissue sections, to reveal the expression pattern of CAR. Statistical analysis was used to determine the correlation between CAR expression and the patient survival rate as a measure of the prognostic value of CAR. RESULTS: CAR was overexpressed in human OSCC tissues (P = .002), and higher expression of CAR was associated with a lower survival rate, which was not statistically significant (P = .123). In addition, patients with OSCC in the human papillomavirus (HPV)-positive group showed significantly higher CAR expression compared with the HPV- negative group (P = .0491). CONCLUSIONS: This study indicated that CAR expression was upregulated in human OSCC and that patients with OSCC with higher expression of CAR had a lower survival rate. Moreover, CAR expression may be associated with HPV infection.

4.
Hum Genet ; 2019 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-31606751

RESUMO

Pluripotent stem cell (PSC) cultures form an integral part of biomedical and medical research due to their capacity to rapidly proliferate and differentiate into hundreds of highly specialized cell types. This makes them a highly useful tool in exploring human physiology and disease. Genomic editing of PSC cultures is an essential method of attaining answers to basic physiological functions, developing in vitro models of human disease, and exploring potential therapeutic strategies and the identification of drug targets. Achieving reliable and efficient genomic editing is an important aspect of using large-scale PSC cultures. The CRISPR/Cas9 genomic editing tool has facilitated highly efficient gene knockout, gene correction, or gene modifications through the design and use of single-guide RNAs which are delivered to the target DNA via Cas9. CRISPR/Cas9 modification of PSCs has furthered the understanding of basic physiology and has been utilized to develop in vitro disease models, to test therapeutic strategies, and to facilitate regenerative or tissue repair approaches. In this review, we discuss the benefits of the CRISPR/Cas9 system in large-scale PSC cultures.

5.
Artigo em Inglês | MEDLINE | ID: mdl-31595169

RESUMO

[This corrects the article DOI: 10.3389/fendo.2019.00007.].

6.
J Exp Med ; 2019 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-31653691

RESUMO

The zinc finger transcription factor, Bcl11b, is expressed in T cells and group 2 innate lymphoid cells (ILC2s) among hematopoietic cells. In early T-lineage cells, Bcl11b directly binds and represses the gene encoding the E protein antagonist, Id2, preventing pro-T cells from adopting innate-like fates. In contrast, ILC2s co-express both Bcl11b and Id2 To address this contradiction, we have directly compared Bcl11b action mechanisms in pro-T cells and ILC2s. We found that Bcl11b binding to regions across the genome shows distinct cell type-specific motif preferences. Bcl11b occupies functionally different sites in lineage-specific patterns and controls totally different sets of target genes in these cell types. In addition, Bcl11b bears cell type-specific post-translational modifications and organizes different cell type-specific protein complexes. However, both cell types use the same distal enhancer region to control timing of Bcl11b activation. Therefore, although pro-T cells and ILC2s both need Bcl11b for optimal development and function, Bcl11b works substantially differently in these two cell types.

7.
Clin Exp Allergy ; 2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31621112

RESUMO

Asthma is a complicated chronic airway inflammatory disorder. In addition to standard steroid treatment, biologic therapies targeting specific cytokines have emerged to reduce exacerbation and improve lung function. Neutralizing antibodies against IL-5, a critical growth factor for eosinophils, reduced asthma exacerbation in patients with severe eosinophilic asthma.1 Blockade of IL4Rα, the receptor to IL-4 and IL-13, also lowered exacerbation rates and improved lung function in moderate to severe uncontrolled asthma.2 While these biologic therapies are now used as add-on maintenance treatment of moderate to severe asthma, the effects of these biologic therapies on human immune responses are yet to be better understood.

8.
Bioresour Technol ; 294: 122235, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31610493

RESUMO

This study compared the effects of sewage sludge-derived pyrochar (PC300, PC500, and PC700) and hydrochar (HC180, HC240, and HC300) on mesophilic anaerobic digestion of waste activated sludge (WAS). It was demonstrated that hydrochar can better promote the methane production compared with pyrochar. The highest accumulative methane yield of 132.04 ±â€¯4.41 mL/g VSadded was obtained with HC180 addition. In contrast, the PC500 and PC700 showed a slightly negative effect on methane production. Sludge-derived HC not only accelerated the solubilization and hydrolysis of sludge floc, but also improved the production of acetic acid and propionate, further resulting in improved methane production. Simultaneously, the syntrophic microbes facilitating direct interspecies electron transfer (DIET) such as Syntrophomonas, Peptococcaceae, Methanosaeta and Methanobacterium bred rapidly with the addition of HCs. These results indicated that the hydrochar is more ideal as the accelerant to promote the methane production from mesophilic anaerobic digestion of WAS than the pyrochar.

9.
World J Gastroenterol ; 25(39): 5973-5990, 2019 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-31660034

RESUMO

BACKGROUND: Long noncoding RNAs (lncRNAs) have been identified to play important roles in the development and progression of various tumors, including gastric cancer (GC). However, the molecular role of lncRNAs in GC progression remains unclear. AIM: To investigate the differential expression of lncRNAs in human GC and elucidate the function and regulatory mechanism of LINC02407. METHODS: The Cancer Genome Atlas database was used to investigate the involvement of lncRNAs in GC. Quantitative real-time polymerase chain reaction was used to estimate the relative expression level of LINC02407 in GC tissues and cells. Functional experiments including CCK8 assay, apoptosis assay, wound healing assay, and transwell assay were used to investigate the effect of LINC02407 on GC cells. Some microRNAs were predicted and verified via bioinformatics analysis and the luciferase reporter system. Predictive analysis and Western blot assay were used to analyze the expression of related proteins. RESULTS: Many differentially expressed lncRNAs were identified in GC, and some of them including LINC02407 can affect the survival. LINC02407 was upregulated in tumor tissues compared with adjacent tissues. HGC-27 cells showed the highest LINC02407 expression and HaCaT cells exhibited the lowest expression. Different experiment groups were constructed using LINC02407 overexpressing plasmids and related siRNAs. The results of functional experiments showed that LINC02407 can promote the proliferation, migration, and invasion of GC cells but inhibit apoptosis. Luciferase reporter assay showed that hsa-miR-6845-5p and hsa-miR-4455 was downstream regulated by LINC02407. Western blot analysis showed that adhesion G protein-coupled receptor D1 (ADGRD1) was regulated by the LINC02407-miR-6845-5p/miR-4455-ADGRD1 pathways. CONCLUSION: LINC02407 plays a role in GC through the LINC02407-miR-6845-5p/miR-4455-ADGRD1 pathways, and thus, it may be an important oncogene and has potential value in GC diagnosis and treatment.

10.
Fish Shellfish Immunol ; 95: 105-116, 2019 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-31610288

RESUMO

The current study aimed to investigate the effects and mechanisms of dietary soybean ß-conglycinin in immune function and oxidative damage among different intestinal segments of juvenile grass carp (Ctenopharyngodon idella). 240 fish (13.77 ±â€¯0.10 g) were fed control or 8% ß-conglycinin diet for 7 weeks. Dietary ß-conglycinin caused inconsistent suppression effects on the innate immune by decreasing complement component, lysozyme, antimicrobial peptide and acid phosphatase among different intestinal segments. Meanwhile, dietary ß-conglycinin caused inflammation in the mid and distal intestine by raising pro-inflammatory cytokines and declining anti-inflammatory cytokines mRNA levels, while more serious in the distal intestine than in the mid intestine. Furthermore, dietary ß-conglycinin regulating inflammatory cytokines might be associated with transcription factors nuclear factor-κB P65 (NF-κB P65) nucleus translocation and target of rapamycin (TOR) phosphorylation in the distal intestine but only related to TOR phosphorylation in the mid intestine. Interestingly, in the proximal intestine, dietary ß-conglycinin decreased both pro-inflammatory and anti-inflammatory cytokines mRNA level, and did not affect NF-κB P65 nucleus translocation and TOR phosphorylation. For oxidative damage, dietary ß-conglycinin exposure elevated both malondialdehyde (MDA) and protein carbonyl (PC) contents in the distal intestine, which might be attributed to the suppression of the Mn-SOD, catalase (CAT) and glutathione peroxidase (GPx) activities. In the mid intestine, dietary ß-conglycinin only increased PC content in association with the low activities of CAT, GPx and glutathione peroxidase (GR). Unexpectedly, in the proximal intestine, dietary ß-conglycinin did not significantly change MDA and PC contents while decreased antioxidant enzyme activities. Furtherly, dietary ß-conglycinin affect the antioxidant enzyme activity might be regulated by the varying pattern of nuclear factor-erythroid 2-related factor 2 (Nrf2) nucleus translocation among these three intestinal segments. In summary, dietary ß-conglycinin caused intestinal inflammation and oxidative damage in association with NF-κB, TOR and Nrf2 signaling molecules, which were varying among the three intestinal segments of grass carp.

11.
J Neurochem ; 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31665810

RESUMO

Cortical areas including the anterior cingulate cortex (ACC) play critical roles in different types of chronic pain. Most of previous studies focus on the sensory inputs from somatic areas, and less information about plastic changes in the cortex for visceral pain. In this study, chronic visceral pain animal model was established by injection with zymosan into the colon of adult male C57/BL6 mice. Whole cell patch-clamp recording, behavioral tests, Western blot, and Cannulation and ACC microinjection were employed to explore the role of adenylyl cyclase 1 (AC1) in the ACC of C57/BL6 and AC1 knock out (AC1 KO) mice. Integrative approaches were used to investigate possible changes of neuronal adenylyl cyclase 1 (AC1) in the ACC after the injury. We found that AC1, a key enzyme for pain-related cortical plasticity, was significantly increased in the ACC in an animal model of irritable bowel syndrome. Inhibiting AC1 activity by a selective AC1 inhibitor NB001 significantly reduced the upregulation of AC1 protein in the ACC. Furthermore, we found that AC1 is required for NMDA GluN2B receptor upregulation and increases of NMDA receptor-mediated currents. These results suggest that AC1 may form a positive regulation in the cortex during chronic visceral pain. Our findings demonstrate that the upregulation of AC1 protein in the cortex may underlie the pathology of chronic visceral pain; and inhibiting AC1 activity may be beneficial for the treatment of visceral pain.

12.
J Hazard Mater ; 384: 121363, 2019 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-31610350

RESUMO

Thiosulfinates, a natural antibiotic, existed in all parts of Allium, therefore might be accumulated in large amounts in food waste (FW). FW was often added into waste activated sludge (WAS) anaerobic digestion process as a kind of supplement for nutrition balance. However, the impact of thiosulfinates on methane production and the possible approach to mitigate its inhibition on the co-digestion process could be available in few literatures. This work was carried out in a series of batch experiment at pH 7.0 ±â€¯0.2 and 35 ±â€¯1.0 ℃ to promote the further understanding of this process. The experimental results showed that the methane accumulation decreased from 270.6 ±â€¯13.4 to 16.7 ±â€¯7.0 mL/g VSS (volatile suspended solids) when the initial concentration of thiosulfinates increased from 0 to 2.5 µg/g VSS. The activities of functional enzymes (F420 and CoM) were inhibited by 99.06% and 99.82% compared with control group when reactor contained 2.5 µg/g VSS thiosulfinates. Furthermore, different temperature, pH, and combination pretreat were applied to impair the inhibition of thiosulfinate. Compared with no pretreatment group, methane yield was increased by 2.26, 32.18 and 42.2-fold, respectively which group was under pretreatment method of heat (100 ℃), alkali (pH 9) and combination.

13.
Appl Opt ; 58(25): 6733-6736, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31503639

RESUMO

We demonstrated a passively Q-switched visible Pr:YLF laser using the gold nanostars (GNSs) as the saturable absorber (SA). The nonlinear saturable absorption properties at 639 nm were measured, and the modulation and saturable fluence were calculated to be 3% and 0.3 GW/cm2, respectively. With the GNSs SA, the efficient passively Q-switched lasers were obtained at 639 nm and 721 nm, respectively. The maximum output powers and shortest pulse widths were (256 mW, 168 ns) at 639 nm and (238 mW, 198 ns) for 721 nm. To the best of our knowledge, this is the first time there has been a visible bulk pulse laser using the GNSs as the SA. Our work indicates the GNSs could be excellent and promising optoelectronic devices in the visible domain.

14.
Artigo em Inglês | MEDLINE | ID: mdl-31526640

RESUMO

To evaluate the efficacy and safety of a new domestic dacron patch in the surgical treatment of congenital heart disease (CHD) with infective endocarditis (IE), a clinical controlled trial is conducted. 48 patients with CHD complicated with IE are selected and randomly divided into two groups. 26 patients in the experimental group are treated with a new domestic dacron patch, while 22 patients in the control group are treated with an imported cardiac polyester patch. By echocardiography, collecting chest X-ray, echocardiography and laboratory examination before and after operation, the residual shunt, cardiac function, liver function, renal function, coagulation function and other related indicators are observed after operation in the two groups, and the therapeutic effect and safety of the new dacron patch are evaluated. The results showed that there is no significant difference in C/T (cardiothoracic ratio), LAD (Left atrial diameter), LVSD (left ventricular end systolic diameter) and LVDD (left ventricular end diastolic diameter) between the two groups before operation, before discharge and 1, 3 and 6 months after discharge (P>0.05). The C/T, LAD and LVDD of the two groups decrease 6 months after operation, and the size of atrioventricle decreases significantly. There is a decreasing trend in the experimental group compared with the control group, but there is no significant difference (P>0.05). There are no significant differences in cardiac function classification, echocardiography, electrocardiogram, patch performance evaluation and blood compatibility between the two groups before operation and 6 months after discharge. CONCLUSION: The new domestic dacron patch has good clinical efficacy and safety.

15.
Virus Res ; 273: 197756, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31521762

RESUMO

Most HIV-1-infected patients experience hematopoiesis suppression complications. Bone marrow mesenchymal stem cells (BMSCs) are involved in regulation of hematopoietic homeostasis, so we investigated the role of Tat, a protein released by infected cells in bone marrow and impacted differentiation potential of mesenchymal stem cells, in the BMSC hematopoietic support function. BMSCs were treated with HIV-1 Tat protein (BMSCTat-p), transfected with HIV-1 Tat mRNA (BMSCTat-m) or treated with solvent (PBS) (BMSCcon) for 20 days. Then, the hematopoietic support function of BMSCTat-p, BMSCTat-m and BMSCcon was analyzed via ex vivo expansion of hematopoietic stem cells (HSCs) grown on the BMSCs and via in vivo cotransplantation of HSCs and BMSCs. In addition, the hematopoiesis-supporting gene expression patterns of BMSCTat-p, BMSCTat-m and BMSCcon were compared. The results showed that BMSCTat-p and BMSCTat-m displayed reduced expansion, a decline in the number of colony forming units (CFUs) and a decreased proportion of the primitive subpopulation of hematopoietic stem cells under coculture conditions compared with BMSCcon. The ability of BMSCTat-p to support hematopoietic recovery was also impaired, which was further confirmed by the patterns in gene expression analysis. In conclusion, Tat treatment reduced the function of BMSCs in hematopoietic support, likely by downregulating the expression of a series of hematopoietic cytokines.

16.
Opt Express ; 27(18): 25902-25911, 2019 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-31510452

RESUMO

A tunable metamaterial absorber is proposed in the terahertz regime. The amplitude and center frequency of the absorber can be tuned independently. Owing to the effective combination of graphene and strontium titanate (STO) in one metamaterial structure, the tunable properties of the amplitude and center frequency are implemented. The amplitude can be tuned by adjusting the chemical potential of graphene sheet, and center frequency can get a shift through temperature changes in the STO material. In a full-wave numerical simulation, the amplitude of the absorber can be tuned from approximately 100% to 35% with a fixed center frequency when chemical potential varies from 0.7 eV to 0.0 eV. The center frequency of the absorber can shift from 0.43 THz to 0.3 THz when temperature changes from 400 K to 200 K. The complex surface impedance of the graphene and permittivity of STO material in this research range are thoroughly examined, and the independently tunable mechanism of the absorber is explored by elucidating the electric field distribution. The influence of the oblique incidence of electromagnetic wave to the absorber is studied. The absorber can be scalable to the infrared and visible frequencies and demonstrates promising application on tunable sensors, filters, and photovoltaic devices.

17.
FEMS Microbiol Ecol ; 95(9)2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31494678

RESUMO

Sponges are complex holobionts in which the structure of the microbiome has seldom been characterized above the host species level. The hypothesis tested in this study is that the structure of the sponge microbiomes is specific to the host at the order and family levels. This was done by using 33 sponge species belonging to 19 families representing five orders. A combination of three primer sets covering the V1-V8 regions of the 16S rRNA gene provided a more comprehensive coverage of the microbiomes. Both the diversity and structure of sponge microbiomes were demonstrated to be highly specific to the host phylogeny at the order and family levels. There are always dominant operational taxonomic units (OTUs) (relative abundance >1%) shared between microbial communities of sponges within the same family or order, but these shared OTUs showed high levels of dissimilarity between different sponge families and orders. The unique OTUs for a particular sponge family or order could be regarded as their 'signature identity'. 70%-87% of these unique OTUs (class level) are unaffiliated and represent a vast resource of untapped microbiota. This study contributes to a deeper understanding on the concept of host-specificity of sponge microbiomes and highlights a hidden reservoir of sponge-associated microbial resources.

18.
Sci Total Environ ; 697: 134096, 2019 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-31476494

RESUMO

Dryland vegetation forms a "fertile island effect" due to water and nutrient retention. However, there has been little research on the mechanism underlying C-, N-, P-accumulation and overall fertile island at the community level. We therefore presented the systematic investigation on this issue through the survey in desert-oasis ecotone. The survey covering the vegetation composition, plant height, crown area and vegetation cover. The main parameters measured included soil moisture, soil pH, soil salinity and nine soil indicators related to C, N and P cycling. The results revealed that the effect of fertile island was directly relevant to either soil moisture or pH. This effect was more obvious with the increase of soil moisture and the decrease of pH value. In addition, the plant diversity was believed to be the main biotic driven factor for fertile island. Furthermore, the results also indicated that both the soil moisture and plant diversity would accelerate the accumulation of P and N, while the pH played the negative effect. The other main observation obtained was that the vegetation cover had positive effect on accumulation of C. As a result, the mechanisms related to drought and salinization could drive the difference of C-, N- and P-accumulation. The main findings also provided an effective reference to better understand the mechanism of fertile island and its desertification procedure in desert-oasis ecotone.

19.
Cancer Res ; 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31484669

RESUMO

The genetic events occuring in recurrent nasopharyngeal carcinoma (rNPC) are poorly understood. Here, we performed whole-genome and whole-exome sequencing in 55 rNPC and 44 primarily diagnosed NPC (pNPC) patients; with 7 patients having paired rNPC and pNPC samples. Previously published pNPC exome data were integrated for analysis. rNPC and pNPC tissues had similar mutational burdens, however, the number of clonal mutations was increased in rNPC samples. TP53 and three NF-κB pathway components (TRAF3, CYLD and NFKBIA) were significantly mutated in both pNPC and rNPC. Notably, mutations in TRAF3, CYLD and NFKBIA were all clonal in rNPC, however, 55.6-57.9% of them were clonal in pNPC. In general, the number of clonal mutations in NF-κB pathway-associated genes was significantly higher in rNPC than in pNPC. The NF-κB mutational clonality was selected and/or enriched during NPC recurrence. The amount of NF-κB translocated to the nucleus in samples with clonal NF-κB mutants was significantly higher than that in samples with subclonal NF-κB mutants. Moreover, the nuclear abundance of NF-κB protein was significantly greater in pNPC samples with local regional relapse than in those without relapse. Further, high nuclear NF-κB levels were an independent negative prognostic marker for locoregional relapse-free survival in pNPC. Lastly, inhibition of NF-κB enhanced both radiosensitivity and chemosensitivity in vitro and in vivo. In conclusion, NF-κB pathway activation by clonal mutations plays an important role in promoting the recurrence of NPC. Moreover, nuclear accumulation of NF-κB is a prominent biomarker for predicting locoregional relapse-free survival.

20.
J Epidemiol ; 2019 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-31474675

RESUMO

Backgroud Establishment of an unbiased association between gestational weight gain (GWG) and perinatal health is urgently needed in China, which has the largest population in the world. Our study aimed to create weight-gain-for-gestational-age charts using early pregnancy body mass index (BMI) to present selected percentiles of GWG in China.Methods A population-based follow-up study was conducted based on the Maternal and Newborn Health Monitoring System, which recruited 132,835 pregnant women between October 2013 and September 2016 in 12 districts/counties of 6 provinces in China. Multilevel analyses and restricted cubic splines were performed to model the longitudinal repeated maternal weight gain measurements and obtain smoothed curves for GWG. The internal and external validation of each model was also assessed.Results To develop models of GWG, 34,288 women were included. Smoothed percentiles of GWG in the 3rd, 10th, 50th, 90th, and 97th percentiles were estimated for each week of gestation. The median figures for GWG were 15.0 kg, 14.4 kg, 13.5 kg, and 12.1 kg in underweight, normal weight, overweight, and obese women, respectively, at 40 weeks. Of all the weight measurements, more than 70% and 95% fell within the expected 1 to 2 standard deviations, respectively. To accomplish external validation of the models, 20,458 women were included. The specificities of measurements in the 5th, 10th, 15th, 25th, 75th, 85th, 90th, and 95th percentiles in four BMI categories were between 90% and 100%.Conclusions The population-based gestational weight gain z-score charts performed well in providing guidance regarding expected gestational weight gain in Chinese women.

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