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1.
J Cancer Res Ther ; 16(5): 1038-1050, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33004745

RESUMO

Aim: This study aimed to investigate the predictive power of the combination of Systemic Immune-Inflammation Index (SII) and albumin-bilirubin (ALBI) grade in prognosis outcomes of early-stage hepatocellular carcinoma (HCC) after thermal ablation. Materials and Methods: This retrospective study was reviewed and approved by our institutional review board, and written informed consent was obtained from each patient. According to the Milan criteria, a total of 405 treatment-naïve patients with clinicopathologically confirmed HCC were enrolled who subsequently underwent thermal ablation from 2011 to 2016. The outcomes of overall survival (OS), cancer-specific survival (CSS), and recurrence-free survival (RFS) were statistically analyzed. Results: The median follow-up time of this study was 45.1 months (range, 1.3-83.2 months). After thermal ablation in patients with SII-ALBI Grades 1, 2, and 3, the cumulative 5-year OS rates were 81.7%, 63.2%, and 26.9%; the 5-year CSS rates were 82.4%, 67.5%, and 26.9%; and the 5-year RFS rates were 49.3%, 44.6%, and 25.3%, respectively (all P < 0.001). On multivariate Cox regression analyses, SII-ALBI was independently associated with the three outcomes after adjustment for various confounders (all P < 0.05). In addition, SII-ALBI played a predictive role in OS, CSS, and RFS for patients with negative alpha-fetoprotein (AFP) (P < 0.05). Compared with SII and ALBI, the AUCs for the prediction of OS and CSS using SII-ALBI were superior to single indicator (bothP < 0.05). Conclusion: Elevated preablation SII-ALBI is associated with shorter OS, CSS, and RFS in patients with early-stage HCC. Our indicator showed the potential to be a supplement tool for patients with negative AFP during follow-up.

2.
Stem Cell Reports ; 15(4): 968-982, 2020 10 13.
Artigo em Inglês | MEDLINE | ID: mdl-33053361

RESUMO

Sertoli cells are the major component of the spermatogonial stem cell (SSC) niche; however, regulatory mechanisms in Sertoli cells that dictate SSC fate decisions remain largely unknown. Here we revealed features of the N6-methyladenosine (m6A) mRNA modification in Sertoli cells and demonstrated the functions of WTAP, the key subunit of the m6A methyltransferase complex in spermatogenesis. m6A-sequencing analysis identified 21,909 m6A sites from 15,365 putative m6A-enriched transcripts within 6,122 genes, including many Sertoli cell-specific genes. Conditional deletion of Wtap in Sertoli cells resulted in sterility and the progressive loss of the SSC population. RNA sequencing and ribosome nascent-chain complex-bound mRNA sequencing analyses suggested that alternative splicing events of transcripts encoding SSC niche factors were sharply altered and translation of these transcripts were severely dysregulated by Wtap deletion. Collectively, this study uncovers a novel regulatory mechanism of the SSC niche and provide insights into molecular interactions between stem cells and their cognate niches in mammals.

3.
J Int Med Res ; 48(10): 300060520961279, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33044871

RESUMO

Black hairy tongue (BHT) is characterized by a discolored, hairy tongue. We herein report two cases of BHT associated with antibacterial agents and review previous cases. In Case 1, a 17-year-old girl with a central neurocytoma was administered intravenous piperacillin-tazobactam for postoperative infection, and BHT developed 12 days later. Her symptoms resolved 8 days after she discontinued the piperacillin-tazobactam and brushed her tongue three times daily. In Case 2, a 65-year-old man was administered intravenous piperacillin-tazobactam and levofloxacin to treat multidrug-resistant Pseudomonas aeruginosa, and BHT developed 15 days later. The piperacillin-tazobactam was discontinued and the patient brushed his tongue, and the discoloration gradually subsided thereafter. However, the BHT reappeared after linezolid treatment. The patient had adverse drug reactions to both the piperacillin-tazobactam and linezolid treatments. The BHT might have been related to antibiotic use in both cases. We identified 19 cases of antibiotic-related BHT in a literature search, but none were related to piperacillin-tazobactam use. In all cases, symptoms resolved after discontinuation of the drug and brushing of the tongue. BHT may be a rare adverse effect of antibiotics. Treatment strategies include removal of the causative agents, mechanical debridement, and good oral hygiene.

4.
Chemosphere ; 264(Pt 2): 128548, 2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-33059291

RESUMO

The electro-assisted autohydrogenotrophic reduction of perchlorate (ClO4-) was investigated in a dual-chamber biofilm-electrode reactor (BER), in which the microbial community was inoculated from natural sediments. To avoid the effect of extreme pH and direct electron transfer on perchlorate reduction, a novel cathode configuration was designed. The pH of the cathode compartment was successfully controlled in the range of 7.2-8.4 during whole experiment. The effective biological autohydrogenotrophic reduction of perchlorate was achieved using hydrogen generated in-situ on the electrode surface, and the removal rate of 10 mg L-1 perchlorate reached 98.16% at HRT of 48 h. The highest perchlorate removal flux reached to 1498.420 mg m-2·d-1 with a 0.410 kW·h g-perchlorate-1 energy consumption. The microbial community evolution in the BER was determined by high-throughput sequencing and the results indicated that the Firmicutes and Bacteroidetes were dominant at phylum level when perchlorate concentration was 10 mg L-1 or lower. And the Proteobacteria became ascendant at the perchlorate concentration of 20 mg L-1. The functional populations for perchlorate reduction were successfully enriched including Nitrosomonas (30%), Thermomonas (9%), Comamonas (8%) and Hydrogenophaga (3%). Meanwhile, the proportion of functional population in biofilm linked to perchlorate concentration. With the increase of influent perchlorate concentration, the perchlorate-reducing bacteria (PRB) were enriched successfully and became ascendant.

5.
Sensors (Basel) ; 20(19)2020 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-32977650

RESUMO

At present, there are two obvious problems in radar-based gait recognition. First, the traditional radar frequency band is difficult to meet the requirements of fine identification with due to its low carrier frequency and limited micro-Doppler resolution. Another significant problem is that radar signal processing is relatively complex, and the existing signal processing algorithms are poor in real-time usability, robustness and universality. This paper focuses on the two basic problems of human gait detection with radar and proposes a human gait classification and recognition method based on millimeter-wave array radar. Based on deep-learning technology, a multi-channel three-dimensional convolution neural network is proposed on the basis of improving the residual network, which completes the classification and recognition of human gait through the hierarchical extraction and fusion of multi-dimensional features. Taking the three-dimensional coordinates, motion speed and intensity of strong scattering points in the process of target motion as network inputs, multi-channel convolution is used to extract motion features, and the classification and recognition of typical daily actions are completed. The experimental results show that we have more than 92.5% recognition accuracy for common gait categories such as jogging and normal walking.

6.
Head Neck ; 2020 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-32985734

RESUMO

BACKGROUND: Surgical access for retropharyngeal lymph node (RPLN) dissection is difficult. We aimed to examine the efficacy of transcervical endoscopic RPLN dissection (TSE-RPLND) for recurrent RPLN in nasopharyngeal carcinoma (NPC). METHODS: From April 2013 to February 2019, a total of 31 patients with NPC diagnosed with RPLN recurrence underwent TSE-RPLND. The clinical characteristics, complications, and survival outcomes were retrospectively analyzed. RESULTS: The mean duration of surgery, quantity of bleeding and postoperative hospitalization stay were 347.9 minutes, 107.7 mL, and 8.7 days, respectively. After a median follow-up of 31.0 months, the 2-year locoregional relapse-free survival, distant metastasis-free survival, progression-free survival, and overall survival rates were 63.9%, 95.2%, 59.9%, and 83.3%, respectively. The long-term incidences of swallowing problems, permanent nutrient tube, tongue atrophy, and shoulder problems were 6 (19.4%), 3 (9.7%), 3 (9.7%), and 3 (9.7%), respectively. CONCLUSIONS: TSE-RPLND is an effective method to treat RPLN recurrence in NPC, but nerve injury-related complications should not be ignored.

7.
Artigo em Inglês | MEDLINE | ID: mdl-32924220

RESUMO

Traditional aqueous zinc-ion batteries (ZIBs) based on ion-intercalation or surface redox behaviors at the cathode side suffer severely from an unsatisfactory specific capacity and unstable output potential. Herein, these issues are applied to a conversion-type zinc-tellurium (Zn-Te) battery. Typically, this battery works based on a two-step solid-to-solid conversion with the successive formation of zinc ditelluride (ZnTe2 ) and zinc telluride (ZnTe). It delivers an ultrahigh volumetric capacity of 2619 mAh cm-3 (419 mAh g-1 ), 74.1% of which is from the first conversion (Te to ZnTe2 ) with an ultraflat discharge plateau. Though reported first in a challenging aqueous environment, this Zn-Te battery demonstrates an excellent capacity retention of >82.8% after 500 cycles, which results from the elimination of the notorious "shuttle effect" due to the solid-to-solid conversion behaviors. In addition, a quasi-solid-state Zn-Te battery is also fabricated, exhibiting superior flexibility, robustness, and good electrochemical performance. This work develops a novel cathode material based on conversion-type ion-storage mechanism. The system is attractive due to its ultrastable energy output, which is rarely reported for ZIBs.

8.
Water Sci Technol ; 82(4): 759-772, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32970627

RESUMO

In recent years, carbon tetrachloride (CT) has been frequently detected in surface water and groundwater around the world; it is necessary to find an effective way to treat wastewater contaminated with it. In this study, Ni/Fe bimetallic nanoparticles were immobilized on reduced graphene oxide (NF@rGO), and used to dechlorinate CT in aqueous solution. Scanning electron microscopy (SEM) demonstrated that the two-dimensional structure of rGO could disperse nanoparticles commendably. The results of batch experiments showed that the 4N4F@rGO (Fe/GO = 4 wt./wt., and Ni/Fe = 4 wt.%) could reach a higher reduction capacity (143.2 mgCT/gcatalyst) compared with Ni/Fe bimetallic nanoparticles (91.7 mgCT/gcatalyst) and Fe0 nanoparticles (49.8 mgCT/gcatalyst) respectively. That benefited from the nickel metal as a co-catalyst, which could reduce the reaction activation energy of 6.59 kJ/mol, and rGO as an electrical conductivity supporting material could further reduce the reaction activation energy of 4.73 kJ/mol as presented in the conceptual model. More complete dechlorination products were generated with the use of 4N4F@rGO. Based on the above results, the reductive pathway of CT and the catalytic reaction mechanism have been discussed.


Assuntos
Ferro , Níquel , Tetracloreto de Carbono , Grafite , Cinética
9.
Vet Res ; 51(1): 125, 2020 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-32988413

RESUMO

The aim of this study was to investigate the biological characteristics and functions of a Trichinella spiralis serine proteinase (TsSerp) during larval invasion and development in the host. The full-length TsSerp cDNA sequence was cloned and expressed in Escherichia coli BL21. The results of RT-PCR, IFA and western blotting analyses showed that TsSerp was a secretory protein that was highly expressed at the T. spiralis intestinal infective larva and muscle larva stages and primarily located at the cuticle, stichosome and intrauterine embryos of the parasite. rTsSerp promoted the larval invasion of intestinal epithelial cells (IECs) and the enteric mucosa, whereas an anti-rTsSerp antibody impeded larval invasion; the promotion and obstruction roles were dose-dependently related to rTsSerp and the anti-rTsSerp antibodies, respectively. Vaccination of mice with rTsSerp elicited a remarkable humoral immune response (high levels of serum IgG, IgG1/IgG2a, IgE and IgM), and it also triggered both systemic (spleen) and local intestinal mucosal mesenteric lymph node (MLN) cellular immune responses, as demonstrated by a significant elevation in Th1 cytokines (IFN-γ) and Th2 cytokines (IL-4) after the spleen and MLN cells from vaccinated mice were stimulated with rTsSerp. Anti-TsSerp antibodies participated in the killing and destruction of newborn larvae via ADCC. The mice vaccinated with rTsSerp exhibited a 48.7% reduction in intestinal adult worms and a 52.5% reduction in muscle larvae. These results indicated that TsSerp participates in T. spiralis invasion and development in the host and might be considered a potential candidate target antigen to develop oral polyvalent preventive vaccines against Trichinella infection.

10.
Mil Med Res ; 7(1): 41, 2020 09 04.
Artigo em Inglês | MEDLINE | ID: mdl-32887670

RESUMO

The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of a rapidly spreading illness, coronavirus disease 2019 (COVID-19), affecting more than seventeen million people around the world. Diagnosis and treatment guidelines for clinicians caring for patients are needed. In the early stage, we have issued "A rapid advice guideline for the diagnosis and treatment of 2019 novel coronavirus (2019-nCoV) infected pneumonia (standard version)"; now there are many direct evidences emerged and may change some of previous recommendations and it is ripe for develop an evidence-based guideline. We formed a working group of clinical experts and methodologists. The steering group members proposed 29 questions that are relevant to the management of COVID-19 covering the following areas: chemoprophylaxis, diagnosis, treatments, and discharge management. We searched the literature for direct evidence on the management of COVID-19, and assessed its certainty generated recommendations using the Grading of Recommendations, Assessment, Development and Evaluation (GRADE) approach. Recommendations were either strong or weak, or in the form of ungraded consensus-based statement. Finally, we issued 34 statements. Among them, 6 were strong recommendations for, 14 were weak recommendations for, 3 were weak recommendations against and 11 were ungraded consensus-based statement. They covered topics of chemoprophylaxis (including agents and Traditional Chinese Medicine (TCM) agents), diagnosis (including clinical manifestations, reverse transcription-polymerase chain reaction (RT-PCR), respiratory tract specimens, IgM and IgG antibody tests, chest computed tomography, chest x-ray, and CT features of asymptomatic infections), treatments (including lopinavir-ritonavir, umifenovir, favipiravir, interferon, remdesivir, combination of antiviral drugs, hydroxychloroquine/chloroquine, interleukin-6 inhibitors, interleukin-1 inhibitors, glucocorticoid, qingfei paidu decoction, lianhua qingwen granules/capsules, convalescent plasma, lung transplantation, invasive or noninvasive ventilation, and extracorporeal membrane oxygenation (ECMO)), and discharge management (including discharge criteria and management plan in patients whose RT-PCR retesting shows SARS-CoV-2 positive after discharge). We also created two figures of these recommendations for the implementation purpose. We hope these recommendations can help support healthcare workers caring for COVID-19 patients.


Assuntos
Quimioprevenção/métodos , Técnicas de Laboratório Clínico/métodos , Infecções por Coronavirus/tratamento farmacológico , Pneumonia Viral/tratamento farmacológico , Adulto , Betacoronavirus , Infecções por Coronavirus/diagnóstico , Infecções por Coronavirus/prevenção & controle , Medicina Baseada em Evidências , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Pandemias/prevenção & controle , Alta do Paciente/normas , Pneumonia Viral/diagnóstico , Pneumonia Viral/prevenção & controle , Guias de Prática Clínica como Assunto
11.
Int J Med Mushrooms ; 22(7): 627-639, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32865920

RESUMO

Ganoderma lucidum is one of the most famous mushrooms in traditional Chinese medicine. At present, the fully utilized parts of G. lucidum are mainly fruiting body and spore powder. The wild and artificially cultivated G. lucidum fruiting body is costly and rare. Therefore, how to improve the utilization of G. lucidum by means of fermentation is worth investigating. The present study was to perform submerged fermentation of G. lucidum and compare the bioactivities of G. lucidum submerged fermentation broth and fruiting body extract. After the extraction and submerged fermentation methods were optimized, the optimum conditions for extraction were determined as ethanol extraction at 80°C with a solid-to-liquid ratio of 1:30, and those for submerged fermentation were cultivation on malt extract medium for 6 days at 30°C. Under the optimum conditions, the antioxidative activity and tyrosinase inhibition rate of the fermentation broth were 1.2-4.1 fold higher than those of the ethanol extract. Cytotoxicity analysis showed that the ethanol and water extracts and the fermentation broth effectively inhibited pancreatic cancer cells and prostate cancer cells, with much smaller effect on nontumor human embryonic kidney (HEK293T). These results demonstrate that the submerged fermentation could improve the utilization value of G. lucidum and the fermentation broth can be used as an antioxidant additive applied in food, drugs, and cosmetics.

12.
Zhongguo Zhen Jiu ; 40(8): 851-6, 2020 Aug 12.
Artigo em Chinês | MEDLINE | ID: mdl-32869594

RESUMO

OBJECTIVE: To observe the effect of electroacupuncture (EA) on neuronal apoptosis in rats with traumatic brain injury (TBI), and to explore the action mechanism of EA on improving the brain nerve function of TBI. METHODS: A total of 88 6-week-old SD rats were randomly divided into a sham operation group, a model group, an EA group and a LY294002+EA group, 22 rats in each group. The TBI model on the left side was established by the improved Feeney's free fall method. After modeling for 24 h, the rats in the EA group and LY294002+EA group were treated with acupuncture at "Baihui" (GV 20) for 10 min and pricking acupuncture at "Shuigou" (GV 26) for 20 s; EA was applied at "Neiguan" (PC 6) and "Zusanli" (ST 36) on the right side (discontinuous wave, 2 Hz of frequency, 1 mA of intensity) for 10 min, once a day for 3 days. After 3 days of intervention, the TUNEL method was used to detect the level of neuron apoptosis in left cerebral cortex; the Western blot method was used to detect the expression of Akt, p-Akt, Bcl-2, Bax, Cyt-C and Caspase-9 in the left cerebral cortex. RESULTS: After 3-day treatment, compared with the sham group, the number of neuronal apoptosis in the left cortex was increased in the model group (P<0.01), and the expression of Bax, Cyt-C and Caspase-9 protein was increased (P<0.01), and the expression of p-Akt/Akt, Bcl-2 was decreased (P<0.01). Compared with the model group, the number of neuronal apoptosis in the left cortex was decreased in the EA group (P<0.01), and the expression of Bax, Cyt-C and Caspase-9 was decreased (P<0.01), and the expression of p-Akt/Akt and Bcl-2 was increased (P<0.01). Compared with the LY294002+EA group, the number of neuronal apoptosis in the left cortex was decreased in the EA group (P<0.01), and the expression of Bax, Caspase-9 and Cyt-C was decreased (P<0.01, P<0.05), and the expression of p-Akt/Akt and Bcl-2 was increased (P<0.01). CONCLUSION: EA could significantly reduce the neuronal apoptosis in rats with TBI, and its mechanism may be related to the activation of PI3K/Akt signaling pathway.

13.
World J Gastroenterol ; 26(30): 4523-4536, 2020 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-32874062

RESUMO

BACKGROUND: No studies have evaluated the predictive value of alarm symptoms for organic dyspepsia and organic upper gastrointestinal (GI) diseases based on Rome IV criteria in the Chinese population. AIM: To evaluate the predictive value of alarm symptoms for dyspeptic patients based on Rome IV criteria. METHODS: We performed a cross-sectional study of dyspepsia patients who met the inclusion and exclusion criteria at two academic urban tertiary-care centers from March 2018 to January 2019. Basic demographic data, dyspeptic information, alarm symptoms, lifestyle, examination results, family history and outpatient cost information were collected. Dyspepsia patients with normal findings on upper GI endoscopy, epigastric ultrasound and laboratory examination and without Helicobacter pylori-associated dyspepsia were classified as functional dyspepsia. RESULTS: A total of 381 patients were enrolled in the study, including 266 functional dyspepsia patients and 115 organic dyspepsia patients. There were 24 patients with organic upper GI disease among patients with organic dyspepsia. We found that based on the Rome IV criteria, alarm symptoms were of limited value in differentiating organic dyspepsia and organic upper GI diseases from functional dyspepsia. Age (odds ratio (OR) = 1.056, P = 0.012), smoking (OR = 4.714, P = 0.006) and anemia (OR = 88.270, P < 0.001) were independent predictors for organic upper GI diseases. For the comparison of epigastric pain syndrome, postprandial distress syndrome and epigastric pain syndrome combined with postprandial distress syndrome, the results showed that there were statistically significant differences in anorexia (P = 0.021) and previous visits (P = 0.012). The ClinicalTrials.gov number is NCT03479528. CONCLUSION: Most alarm symptoms had poor predictive value for organic dyspepsia and organic upper GI diseases based on Rome IV criteria. Gastroscopic screening should not be based solely on alarm symptoms.

14.
Animals (Basel) ; 10(9)2020 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-32962114

RESUMO

In the mammalian testes, Sertoli cells are the only somatic cells in the seminiferous tubules that provide structural, nutritional and regulatory support for developing spermatogenic cells. Sertoli cells only proliferate during the fetal and neonatal periods and enter a quiescent state after puberty. Functional evidences suggest that the size of Sertoli cell population determines sperm production and fertility. However, factors that direct Sertoli cell proliferation and maturation are not fully understood. Transcription factor E4F1 is a multifunctional protein that serves essential roles in cell fate decisions and because it interacts with pRB, a master regulator of Sertoli cell function, we hypothesized that E4F1 may have a functional role in Sertoli cells. E4f1 mRNA was present in murine testis and immunohistochemical staining confirmed that E4F1 was enriched in mature Sertoli cells. We generated a conditional knockout mouse model using Amh-cre and E4f1flox/flox lines to study E4F1 fucntion in Sertoli cells and the results showed that E4f1 deletion caused a significant reduction in testis size and fertility. Further analyses revealed that meiosis progression and spermiogenesis were normal, however, Sertoli cell proliferation was impaired and germ cell apoptosis was elevated in the testis of E4f1 conditional knockout mice. On the basis of these findings, we concluded that E4F1 was expressed in murine Sertoli cells and served important functions in regulating Sertoli cell proliferation and fertility.

15.
Artigo em Inglês | MEDLINE | ID: mdl-32965789

RESUMO

Introducing pseudocapacitive behavior and ion hybrid capacitor technique were deemed effective to improve the energy density of supercapacitors. However, the current research about ion hybrid capacitor only considered the reaction of cations during the electrochemical process, leading to a flawed mechanism understanding. Here, the effects of various anions carriers on the electrochemical behaviors of titanium nitride-based zinc ion capacitor (Zn-TiN capacitor) have been carefully explored. Density functional theory (DFT) calculation results revealed the stable structure of TiN-SO 4 after adsorbed process, enabling SO 4 2- participate in the electrochemical process and construct a two-step adsorption and intercalation energy storage mechanism, which greatly improved the capacitance and anti-self-discharge ability of Zn-TiN capacitor. Zn-TiN capacitor delivered an ultrahigh capacitance of 489.8 F g -1 and remained 83.92% of capacitance even after 500 h resting time. It is believed that designing an appropriate energy storage system to involve anions in the electrochemical process can significantly improve the capacitance and anti-self-discharge ability of ion hybrid capacitors.

16.
Zhen Ci Yan Jiu ; 45(9): 714-9, 2020 Sep 25.
Artigo em Chinês | MEDLINE | ID: mdl-32959553

RESUMO

OBJECTIVE: To investigate the expression of apoptosis-related proteins Fas and FasL in the brain tissue of rats with traumatic brain injury and the effect of electroacupuncture on the expression of Fas and FasL, so as to explore the effective time window of electroacupuncture in the treatment of traumatic brain injury. METHODS: Sprague-Dawley rats were randomly divided into blank group, sham-operation group, model group, and electroacupuncture treatment groups 1, 2, and 3. Traumatic brain injury was induced by the modified Feeney free-fall impact device, and for the rats in the electroacupuncture treatment groups 1, 2, and 3, electroacupuncture started at 4 hours and on days 3 and 7, respectively, after modeling and lasted to day 14. The Morris water maze test was used to evaluate learning and memory ability, and immunofluorescence assay and Western blot were used to observe the changes in the expression of Fas and FasL in traumatic brain tissue. RESULTS: Compared with the blank group and the sham-operation group, the model group had a lower percentage of time spent in the target quadrant from the 3rd day folowing modeling; after electroacupuncture intervention, the electroacupuncture treatment groups showed a gradual increase in the time spent in the target quadrant, and on day 7,10 and 14, electroacupuncture treatment group 1 had a significantly higher percentage than the model group (P<0.05). On day 14, electroacupuncture treatment group 2 had a significantly higher percentage than the model group (P<0.05). After electroacupuncture intervention, all groups except the blank group and the sham-operation group had increases in the expression of Fas and FasL in brain tissue, which reached the highest level on day 7 after modeling and then tended to decrease; compared with electroacupuncture treatment groups 2 and 3 and the model group, electroacupuncture treatment group 1 had significant reductions in the expression of Fas and FasL (P<0.05, P<0.01); compared with electroacupuncture treatment group 3 and the model group, electroacupuncture treatment group 2 had significant decreases in the expression of Fas and FasL (P<0.05) on day 14 after modeling; compared with the model group, electroacupuncture treatment group 3 had significant reductions in the expression of Fas and FasL in brain tissue on day 14 after modeling (P<0.05). CONCLUSION: Early electroacupuncture intervention can regulate the apoptosis receptor pathway by down-regulating Fas and FasL to exert a therapeutic effect on traumatic brain injury and help with the recovery of cognition and memory ability after traumatic brain injury.

17.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 37(10): 1104-1107, 2020 Oct 10.
Artigo em Chinês | MEDLINE | ID: mdl-32924111

RESUMO

OBJECTIVE: To determine the carrier rate of Fragile X mental retardation 1 gene (FMR1) mutants in women with a history of adverse pregnancy or childbirth, and to provide prenatal diagnosis for the carriers. METHODS: Peripheral blood samples were collected from women with a history of adverse pregnancy or childbirth, and the FMR1 gene cytosine-guanine-guanine repeat number (CGG)n was determined by triple-repeat primer polymerase chain reaction (TP-PCR) combined with capillary electrophoresis. Prenatal diagnosis was provided for the carriers during pregnancy. RESULTS: Among 819 samples, 9 gray zone repeats carriers and 10 premutation carriers were detected, which gave a prevalence of 1 in 91 and 1 in 82, respectively, with a total prevalence of 1 in 43. Prenatal diagnosis was provided during 7 pregnancies for 6 carriers. A female fetus with premutation (n = 30/57) and an affected male fetus with full mutation (n = 336) were detected. CONCLUSION: FMR1 gene testing in women with a history of adverse pregnancy or childbirth can facilitate genetic counseling and reproductive guidance for carriers of gray zone repeats and premutations. Prenatal diagnosis for carriers of premutation can facilitate reduction of the birth of children with fragile X syndrome.

18.
Pathol Int ; 2020 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-32940948

RESUMO

Malignant tumors presented in hernia sac are very rare and the clinicopathological features are not well studied. We retrospectively reviewed 21 patients with cancers identified in surgically resected hernia sac specimens at our institution, including 14 males and 7 females, with a median age of 65 years (range 35-93). Fourteen patients (66.7%) carried a synchronous or metachronous cancer diagnosis elsewhere and the tumors in hernia sac were proven to be metastasis. Of the remaining seven (33.3%) cases, hernia sac tumors were the initial presentation of malignancy, and a panel of immunohistochemistry markers were applied in combination with clinical information to identify tumor origin. The final classification of all 21 cases was as follows: 11 gastrointestinal tract tumors including 10 adenocarcinomas and 1 gastrointestinal stromal tumor, 5 pancreatobiliary adenocarcinomas and 5 gynecological serous carcinomas. Compared to other tumor types, pancreatobiliary adenocarcinomas were more likely to involve hernia sac as initial presentation before a primary site was identified (P = 0.0307) and had worse survival (P < 0.005). As the first and largest cohort of malignant tumors in hernia sac, our study provides critical data on frequency, distribution, clinicopathological features and applicability of immunohistochemical markers to determine tumor origin in cases with unknown primary.

19.
Int J Biol Macromol ; 165(Pt A): 44-49, 2020 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-32987075

RESUMO

Starch is a basic biomacromolecule, and an in-depth understanding of the process and mechanism of starch-lipid complexation has great significance for starch based food and pharmaceutical. In this study, molecular dynamics simulation was used to explore the complexation details between starch molecules and trilinolenin, such as complexation process, interaction forces, conformation changes and stability changes, which are difficult to be verified by using other characterization methods. The results show that, firstly, starch residues of one turn helix (8 residues) are enough to bind a trilinolenin molecule firmly. Secondly, the complex is maintained by Van der Waals and electrostatic interaction. Thirdly, the residues complexed with trilinolenin become more stable than the former or the free residues. In brief, the complexation process, interaction forces, conformation changes and stability changes of the starch-trilinolenin complex were clarified in this study. The results may create new insights for the research about the interaction of starch and lipid, then provide theoretical guidance for the research on starch based food and pharmaceutical.

20.
Cancer Commun (Lond) ; 40(10): 518-530, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32888253

RESUMO

BACKGROUND: Glioma, the most frequent primary tumor of the central nervous system, has poor prognosis. The epidermal growth factor receptor (EGFR) pathway and angiogenesis play important roles in glioma growth, invasion, and recurrence. The present study aimed to use proteomic methods to probe into the role of the EGF-EGFR-angiogenesis axis in the tumorigenesis of glioma and access the therapeutic efficacy of selumetinib on glioma. METHODS: Proteomic profiling was used to characterize 200 paired EGFR-positive and EGFR-negative glioma tissues of all pathological types. The quantitative mass spectrometry data were used for systematic analysis of the proteomic profiles of 10 EGFR-positive and 10 EGFR-negative glioma cases. Consensus-clustering analysis was used to screen target proteins. Immunofluorescence analysis, cell growth assay, and intracranial xenograft experiments were used to verify and test the therapeutic effect of selumetinib on glioma. RESULTS: Advanced proteomic screening demonstrated that the expression of EGF-like domain multiple 7 (EGFL7) was higher in EGFR-positive tumor tissues than in EGFR-negative tumor tissues. In addition, EGFL7 could act as an activator in vitro and in vivo to promote glioma cell proliferation. EGFL7 was associated strongly with EGFR and prognosis. EGFL7 knockdown effectively suppressed glioma cell proliferation. Selumetinib treatment showed tumor reduction effect in EGFR-positive glioblastoma xenograft mouse model. CONCLUSIONS: EGFL7 is a potential diagnostic biomarker and therapeutic target of glioma. Selumetinib could target the EGFR pathway and possibly improve the prognosis of EGFR-positive glioma.

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