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1.
Gut ; 2021 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-33408122

RESUMO

OBJECTIVE: Data regarding the real-world effectiveness and safety of sofosbuvir/velpatasvir (SOF/VEL) with or without low-dose ribavirin (RBV) in patients with chronic hepatitis C virus (HCV) infection and severe renal impairment (RI) are limited. We evaluated the performance of SOF/VEL with or without low-dose RBV in HCV-infected patients with chronic kidney disease stage 4 or 5. DESIGN: 191 patients with compensated (n=181) and decompensated (n=10) liver diseases receiving SOF/VEL (400/100 mg/day) alone and SOF/VEL with low-dose RBV (200 mg/day) for 12 weeks were retrospectively recruited at 15 academic centres in Taiwan. The effectiveness was determined by sustained virological response at off-treatment week 12 (SVR12) in evaluable (EP) and per-protocol populations (PP). The safety profiles were assessed. RESULTS: The SVR12 rates by EP and PP analyses were 94.8% (95% CI 90.6% to 97.1%) and 100% (95% CI 97.9% to 100%). In patients with compensated liver disease, the SVR12 rates were 95.0% and 100% by EP and PP analyses. In patients with decompensated liver disease, the SVR12 rates were 90.0% and 100% by EP and PP analyses. Ten patients who failed to achieve SVR12 were attributed to non-virological failures. Among the 20 serious adverse events (AEs), none were judged related to SOF/VEL or RBV. The AEs occurring in ≥10% included fatigue (14.7%), headache (14.1%), nausea (12.6%), insomnia (12.0%) and pruritus (10.5%). None had ≥grade 3 total bilirubin or alanine aminotransferase elevations. CONCLUSION: SOF/VEL with or without low-dose RBV is effective and well-tolerated in HCV-infected patients with severe RI.

2.
Stem Cell Res Ther ; 12(1): 18, 2021 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-33413642

RESUMO

BACKGROUND: Steroid-induced osteonecrosis of the femoral head (SONFH) is a devastating orthopedic disease, which seriously affects the quality of life of patients. The study aims to investigate the effects of LncRNA NORAD on SONFH. METHODS: Human bone marrow-derived mesenchymal stem cells (hBMSCs) were isolated from the proximal femur of patients during routine orthopedic surgery and then cultured with dexamethasone (Dex) and transfected with NORAD overexpression vector, siRNA-NORAD and miR-26a-5p mimics. The mRNA expression of NORAD, miR-26a-5p, OPG, RANK, and RANKL was detected by RT-qPCR. Cell proliferation and apoptosis was measured by CCK-8 assay and flow cytometry, respectively. The protein expression of RUNX2, OPG, RANK, and RANKL was detected by western blot. The dual-luciferase reporter gene assay was performed to confirm the binding between NORAD and miR-26a-5p. RESULTS: NORAD expression was downregulated in SONFH tissues, while miR-26a-5p expression was upregulated. Overexpression of NORAD improved DEX-induced inhibition of proliferation and differentiation, and promotion of apoptosis in hBMSCs, while knockdown of NORAD led to the opposite results. Moreover, NORAD improved DEX-induced inhibition of proliferation and differentiation, and promotion of apoptosis by regulation of miR-26a-5p in hBMSCs. CONCLUSIONS: NORAD expression was downregulated in SONFH tissues, while miR-26a-5p expression was upregulated. NORAD improved DEX-induced inhibition of proliferation and differentiation, and promotion of apoptosis by regulation of miR-26a-5p in hBMSCs.

3.
J Agric Food Chem ; 2021 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-33417444

RESUMO

This study aimed to examine the combined use of bone morphogenetic protein-2 (BMP-2) and polysaccharide isolated from Stichopus japonicus on osteogenic differentiation of MC3T3-E1 cells. Osteogenic differentiation was measured via histochemical staining of alkaline phosphatase (ALP) assay, alizarin red staining of mineralization assay, Western blotting, ELISA, and a qRT-PCR evaluation for the expression of BMP-2, runt-related transcription factor-2 (Runx-2), osteocalcin (OCN), osteopontin (OPN), and collagen type I (Col I) in MC3T3-E1 cells. Immunofluorescence assay was utilized to assess the BMP-2 localized on the cell surface. The results illustrated that SP-2 was able to increase ALP expression and accelerate the mineralization. Osteoblasts cultured on BMP-2/SP-2 substrate increased the expression levels of BMP-2, Runx-2, Col I, OCN, and OPN. SP-2 increased the binding efficiency involving a BMP-2 and its cell surface receptor. The dose of 5 µg/mL SP-2 used showed the best function of inducing osteoblast differentiation. These findings indicated that SP-2 is a more effective enhancer that cooperated with BMP-2 to induce osteoblastic differentiation by utilizing the BMP-2 signaling pathway.

4.
J Virol ; 2021 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-33472933

RESUMO

Tembusu Virus (TMUV) is an emerging and re-emerging zoonotic pathogen that adversely affects poultry industry in recent years. TMUV disease is characterized by nonsuppurative encephalitis in ducklings. The duckling infection model was established to study the mechanism of TMUV crossing the blood-brain barrier (BBB) into the central nervous system (CNS). Here, we showed that no obvious clinical symptoms and enhancement of BBB permeability occurred at the early stage of infection (3∼5 dpi). While simultaneously virus particles were observed by transmission electron microscopy in the brain, inducing the accumulation of inflammatory cytokines. Neurological symptoms and disruption of BBB appeared at the intermediate stage of infection (7∼9 dpi). It was confirmed that TMUV could survive and propagate in brain microvascular endothelial cells (BMECs), but did not affect the permeability of BBB in vivo and in vitro at an early date. In conclusion, TMUV enters the CNS then causes encephalitis, and finally destruct the BBB, which may be due to the direct effect of TMUV on BMECs and the subsequent response of "inflammatory storm".IMPORTANCE The TMUV disease has caused huge losses to the poultry industry in Asia, which is potentially harmful to public health. Neurological symptoms and their sequelae are the main characters of this disease. However, the mechanism of how this virus enters the brain and causes encephalitis is unclear. In this study, we confirmed that the virus entered the CNS and then massively destroyed BBB and the BBB damage was closely associated with the subsequent outbreak of inflammation. TMUV may enter the CNS through the transcellular and "Trojan horse" pathways. These findings can fill the knowledge gap in the pathogenesis of TMUV-infected poultry and be benefit for the treatment of TMUV disease. What's more, TMUV is a representative to study the infection of avian flavivirus. Therefore, our studies have significances both for understanding of the full scope of mechanisms of TMUV and other flavivirus infection, and conceivably, for therapeutics.

5.
Artigo em Inglês | MEDLINE | ID: mdl-33484049

RESUMO

MXene (e.g. Ti3C2) represents an important class of two-dimensional (2D) materials owing to its unique metallic conductivity and tunable surface chemistry. However, the mainstream synthetic methods rely on the chemical etching of MAX powders (e.g. Ti3AlC2) using hazardous HF or alike, leading to MXene sheets with fluorine termination and poor ambient stability in colloidal dispersions. Here, we demonstrate a fluoride-free, iodine (I2) assisted etching route for preparing 2D MXene (Ti3C2Tx, T = O, OH) with oxygen-rich terminal groups and intact lattice structure. More than 71 % of sheets are thinner than 5 nm with an average size of 1.8 µm. They present excellent thin-film conductivity of 1250 S cm-1 and great ambient stability in water for at least 2 weeks. 2D MXene sheets with abundant oxygen surface groups are excellent electrode materials for supercapacitors, delivering a high gravimetric capacitance of 293 F g-1 at a scan rate of 1 mV s-1, superior to those made from fluoride-based etchants (< 290 F g-1 at 1 mV s-1). Our strategy provides a promising pathway for the facile and sustainable production of highly stable MXene materials.

6.
Org Lett ; 2021 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-33439028

RESUMO

Three emissive bridged-triphenylamine derivatives are designed and synthesized by incorporating carbon (DQAO), oxygen (OQAO), and sulfur (SQAO) atoms with two carbonyl groups. The fully bridged geometry and unique frontier molecular orbital distribution reveal its potential as narrowband thermally activated delayed fluorescence emitters. DQAO-, OQAO-, and SQAO-based organic light-emitting diodes exhibit the maximum external quantum efficiency (EQEmax) of 15.2%, 20.3%, and 17.8% for blue, green, and yellow, respectively.

7.
J Cell Mol Med ; 2021 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-33439503

RESUMO

Xanthatin (Xa) is a bicyclic sesquiterpene lactone identified from the plant Xanthium L. with impressive antitumor activity, but the role of Xa in non-small cell lung cancer (NSCLC) is not known. Here we found that Xa inhibits proliferation, migration, invasion and induces apoptosis in NSCLC cells. RNA sequencing and Gene set enrichment analysis revealed that Xa significantly activates p53 pathway and suppresses E2F targets, G2M checkpoint and MYC targets in A549 cells. Among these changed genes, the down-regulated gene BARD1 triggered by Xa was identified as a candidate involved in Xa's antitumor effect because of its vital role in homologous recombination (HR). Further studies demonstrated that Xa inhibits HR through the BARD1/BRCA1/RAD51 axis, which enhances cell sensitivity to cisplatin. Mechanistic studies showed that Xa inhibits BARD1 through the JAK2/STAT4 pathway. Our study revealed that Xa is a promising drug to treat NSCLC, especially in combination with conventional chemotherapy.

8.
Spectrochim Acta A Mol Biomol Spectrosc ; 245: 118887, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-32927301

RESUMO

Developing a reliable fluorescence probe is crucial for accurately monitoring sodium dithionite (Na2S2O4, SDT) in biosystems, but the current reported azo-based ones suffers from short excitation/emission wavelengths and relative slow response speed. To address this issue, we herein present a novel near-infrared emissive fluorescence probe for SDT, namely DCM-MQ, consisting of a dicyanomethylene-benzopyran fluorogenic reporter and a 1-methylquinolinium as recognition moiety. On the basis of the specific reduction mechanism, DCM-MQ exhibited a rapid colorimetric and fluorescent recognition for SDT (less than 3 s) with large Stokes shift (112 nm) and high sensitivity (detection limit was 19 nM). The fluorescence imaging results demonstrate that DCM-MQ is competent for monitoring SDT in living systems.

9.
Rev Sci Instrum ; 91(11): 114703, 2020 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-33261458

RESUMO

A rail-gap switch with a multistep triggering system was developed. The rail-gap switch consisted of two rail-like electrodes and a knife-edge electrode parallel to each other. It was assembled from many pieces and filled with unpressurized-flowing dry air. Good alignments between all electrodes were achieved by using a special jig and the knife-edge electrode as the spatial reference. Furthermore, to trigger the rail-gap switch, a multistep triggering system was used. The triggering system consisted of three components: an optical trigger-pulse generator, a slow high-voltage trigger-pulse generator using an ignition coil for a car, and finally, a fast high-voltage trigger-pulse generator using a three-stage Marx generator. The triggering system generated a negative high-voltage trigger pulse of less than -40 kV with a falling speed of -6.6 ± 0.4 kV/ns. The falling speed was fast enough to initiate multichannel discharges in the rail-gap switch. Finally, the rail-gap switch was tested using a test bench consisting of a 0.5-µF capacitor bank charged to 20 kV. The rail-gap switch was triggered by the multistep triggering system robustly with a delay of 180 ns. The delay between the time, when the peak current of the test bench occurred, and the trigger pulse was 890 ns with a jitter of 20 ns, i.e., ∼2% uncertainty in time. The inductance of the rail-gap switch was ∼80 nH obtained from the discharge tests. The rail-gap switch with the multistep high-voltage triggering system is suitable for any pulsed-power systems with current rise times in the order of 1 µs.

10.
J Interpers Violence ; : 886260520980386, 2020 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-33323032

RESUMO

Workplace violence (WPV) in the health sector is a global public health issue. The magnitude of WPV is a particular concern in China's health system. To examine the potential causes of WPV, we analyzed 3,045 qualitative responses to an open-ended question in a survey with health workers in the Zhejiang province, China. We adapted a four-level socio-ecological framework (societal/systemic, community/organizational, interpersonal, and individual) to thematically analyze the data. Ten sub-themes emerged. Within the societal/systemic level, we identified three sub-themes: (a) lack of legislation against WPV, (b) suboptimal accessibility and affordability of health services due to maldistributed health resources, commercialized health services, and inadequate health insurance, and (c) unregulated mass media reports. Within the community/organizational level, three sub-themes emerged: (a) lack of supportive health facility leadership, (b) inaction by government authorities, and (c) inefficient law enforcement agencies. Within the interpersonal level, two sub-themes were identified: (a) poor provider-patient communication and (b) distrust between health workers and patients. Finally, we identified the personal characteristics of health workers (e.g., competence and professionalism) and patients (e.g., sociodemographic background and expectations/satisfaction) as two individual-level sub-themes.We recognized interactions among different levels. The weak state of Chinese legislation in this area and lack of high-level political will and guidance (societal/systemic) has left health facilities and law enforcement agencies (community/organizational) unclear about how to address WPV. The maldistribution of quality health resources (societal/systemic) has led to overcrowded outpatient clinics at higher-level care facilities (community/organizational). In light of the insufficient government funding and profit-oriented health services (societal/systemic), health workers were motivated to seek profits by providing unnecessary services, which compromised their professionalism (individual). Provider-patient relationships deteriorated (interpersonal), and patients sometimes held unrealistically high expectations associated with high medical expenses (individual). We propose multisectoral prevention strategies to address WPV in the health sector at all levels using a socio-ecological framework.

11.
Front Immunol ; 11: 607543, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33324423

RESUMO

Glycogen synthase kinase-3 (GSK3), a cytoplasmic serine/threonine-protein kinase involved in a large number of key cellular processes, is a little-known signaling molecule in virus study. In this study, a GSK3 protein which was highly similar to GSK3ß homologs from other species in Litopenaeus vannamei (designated as LvGSK3ß) was obtained. LvGSK3ß was expressed constitutively in the healthy L. vannamei, at the highest level in the intestine and the lowest level in the eyestalk. White spot syndrome virus (WSSV) reduced LvGSK3ß expression was in immune tissues including the hemocyte, intestine, gill and hepatopancreas. The inhibition of LvGSK3ß resulted in significantly higher survival rates of L. vannamei during WSSV infection than the control group, and significantly lower WSSV viral loads in LvGSK3ß-inhibited L. vannamei were observed. Knockdown of LvGSK3ß by RNAi resulted in increases in the expression of LvDorsal and several NF-κB driven antimicrobial peptide (AMP) genes (including ALF, PEN and crustin), but a decrease in LvCactus expression. Accordingly, overexpression of LvGSK3ß could reduce the promoter activity of LvDorsal and several AMPs, while the promoter activity of LvCactus was increased. Electrophoretic mobility shift assays (EMSA) showed that LvDorsal could bind to the promoter of LvGSK3ß. The interaction between LvGSK3ß and LvDorsal or LvCactus was confirmed using co-immunoprecipitation (Co-IP) assays. In addition, the expression of LvGSK3ß was dramatically reduced by knockdown of LvDorsal. In summary, the results presented in this study indicated that LvGSK3ß had a negative effect on L. vannamei by mediating a feedback regulation of the NF-κB pathway when it is infected by WSSV.

12.
Spine (Phila Pa 1976) ; Publish Ahead of Print2020 Dec 17.
Artigo em Inglês | MEDLINE | ID: mdl-33337679

RESUMO

STUDY DESIGN: Retrospective cohort study. OBJECTIVE: We aimed to study and interpret the morphological features of the lateral atlantoaxial joints (LAJs) in patients with old type II odontoid fractures and atlantoaxial dislocation (AAD). SUMMARY OF BACKGROUND DATA: The causes and consequences of the morphological changes of the LAJs in patients with old type II odontoid fractures and AAD have not been analysed before. METHODS: A retrospective study was performed on patients with old type II odontoid fracture and AAD. These patients were placed in group A (unstable or reducible dislocation) or group B (irreducible or bony dislocation) depending on the type of dislocation they had. We documented the morphological changes and measured the inclination angle of the articular surface of the LAJs in computerized tomographic images and compared the results collected for the two groups. The association between the history of injury and the inclination angle of the articular surface of the LAJs was assessed using the Pearson correlation coefficient. RESULTS: A total of 29 patients were enrolled (16 patients in group A, and 13 patients in group B). The inclination angle in group A was significantly greater than that in group B (P < 0.001). Patients in group B were more likely to have fish-lip-like changes, dome-like changes, or collapse of the LAJs. Age, history, ADI and JOA score were also significant differences between the two groups (P < 0.05). There was a negative correlation between the history of injury and the inclination angle of the articular surfaces of the LAJs(left: r=-0.726, P < 0.001; right: r=-0.795, P < 0.001). CONCLUSIONS: The morphological changes of the LAJs could progressively become more pronounced during the evolution of the disease.Level of Evidence: 4.

13.
Artigo em Inglês | MEDLINE | ID: mdl-33382663

RESUMO

As to unsupervised learning, most discriminative information is encoded in the cluster labels. To obtain the pseudo labels, unsupervised feature selection methods usually utilize spectral clustering to generate them. Nonetheless, two related disadvantages exist accordingly: 1) the performance of feature selection highly depends on the constructed Laplacian matrix and 2) the pseudo labels are obtained with mixed signs, while the real ones should be nonnegative. To address this problem, a novel approach for unsupervised feature selection is proposed by extending orthogonal least square discriminant analysis (OLSDA) to the unsupervised case, such that nonnegative pseudo labels can be achieved. Additionally, an orthogonal constraint is imposed on the class indicator to hold the manifold structure. Furthermore, ℓ2,1 regularization is imposed to ensure that the projection matrix is row sparse for efficient feature selection and proved to be equivalent to ℓ2,0 regularization. Finally, extensive experiments on nine benchmark data sets are conducted to demonstrate the effectiveness of the proposed approach.

14.
Faraday Discuss ; 2020 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-33290460

RESUMO

Flexible electrodes with robust mechanical properties and high electrochemical performance are of significance for the practical implementation of flexible batteries. Here we demonstrate a general and straightforward co-assembly approach to prepare flexible electrodes, where electrochemically exfoliated graphene (EG) is exploited as the film former/conducting matrix and different binary metal oxides (Li4Ti5O12, LiCoO2, Li2MnO4, LiFePO4) are incorporated. The resultant EG-metal oxide hybrids exhibit a unique layer-interlocked structure, where the metal oxide is conformably wrapped by the highly flexible graphene. Due to numerous contact interphases generated between EG and the intercalated material, the hybrid films show high flexibility and can endure rolling, bending, folding and even twisting. When serving as the anode for Li-ion batteries, the freestanding EG-Li4Ti5O12 hybrid presents a characteristic flat discharge plateau at 1.55 V (vs. Li/Li+), indicating transformation of Li4Ti5O12 to Li7Ti5O12. Small polarization, high rate capability and excellent cycling stability against mechanical bending are also demonstrated for the prepared EG-Li4Ti5O12 hybrid. Finally, full cells composed of EG-Li4Ti5O12 and EG-LiFePO4 hybrids show impressive cycling (98% capacity retention after 100 cycles at 1C) and rate performance (84% capacity retained at 2.5C). The straightforward co-assembly approach based on EG can be extended to other two-dimensional layered materials for constructing highly efficient flexible energy storage devices.

15.
Cancer Biol Med ; 17(4): 896-909, 2020 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-33299642

RESUMO

Chemotherapy-induced neutropenia (CIN) is a potentially fatal and common complication in myelosuppressive chemotherapy. The timing and grade of CIN may play prognostic and predictive roles in cancer therapy. CIN is associated with older age, poor functional and nutritional status, the presence of significant comorbidities, the type of cancer, previous chemotherapy cycles, the stage of the disease, specific chemotherapy regimens, and combined therapies. There are many key points and new challenges in the management of CIN in adults including: (1) Genetic risk factors to evaluate the patient's risk for CIN remain unclear. However, these risk factors urgently need to be identified. (2) Febrile neutropenia (FN) remains one of the most common reasons for oncological emergency. No consensus nomogram for FN risk assessment has been established. (3) Different assessment tools [e.g., Multinational Association for Supportive Care in Cancer (MASCC), the Clinical Index of Stable Febrile Neutropenia (CISNE) score model, and other tools] have been suggested to help stratify the risk of complications in patients with FN. However, current tools have limitations. The CISNE score model is useful to support decision-making, especially for patients with stable FN. (4) There are still some challenges, including the benefits of granulocyte colony stimulating factor treatment and the optimal antibiotic regimen in emergency management of FN. In view of the current reports, our group discusses the key points, new challenges, and management of CIN.

16.
Cancer Manag Res ; 12: 11515-11522, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33204162

RESUMO

Background/Aim: TP53 mutation is recognized as a negative prognostic factor for patients with diffuse large B-cell lymphoma (DLBCL). Here, we present the characteristics of TP53 mut DLBCL patients following investigation of the effect of a treatment approach on survival of TP53 mut DLBCL patients. Methods: A total of 44 DLBCL patients with TP53 mut and treated with an R-CHOP regimen were included for analysis. Patients who failed to achieve a complete response (CR) to initial treatment or relapsed in the first 6 months after initial CR were deemed to have primary refractory disease. Results: Among 44 patients harboring TP53 mutations who underwent upfront R-CHOP or R-CHOP-like treatment, 21 (47.7%) had limited-stage and 23 (52.3%) presented advanced-stage disease. Apart from the seven patients receiving upfront surgical resection, 37 had measurable disease under the R-CHOP regimen, with 59.1% (n=26) developing primary refractory disease. Seven limited-stage patients after early complete resection and one with residue resection remained event-free at median follow-up of 37 months. Multivariate analysis revealed that elevated baseline lactate dehydrogenase (LDH), extranodal involvement (two or more), Ann Arbor stage, and locoregional treatment (surgery or radiation therapy) were independent indicators for progression-free survival (PFS). After adjustment for baseline LDH and extranodal involvement, adding locoregional treatment including surgery and radiation to the R-CHOP regimen significantly improved PFS (p=0.008) and overall survival (p=0.017) in limited-stage TP53 mut DLBCL patients compared to R-CHOP-only treatment. Conclusion: This study presents the characteristics of TP53-mutated DLBCL and implies a potential benefit of locoregional treatment in limited-stage DLBCL patients with TP53 mutations.

17.
Cancer Manag Res ; 12: 10975-10984, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33154676

RESUMO

Objective: To investigate the effects of miR-490-3p on the proliferation, migration, invasion and apoptosis of lung adenocarcinoma (LUAD) cells through the Wnt/ß-catenin signaling pathway. Methods: Differentially expressed miRNAs in LUAD tissues were analyzed by bioinformatics and the target miRNA went through GSEA enrichment analysis. qRT-PCR was used to detect the expression of miR-490-3p in human LUAD cells and normal bronchial cells. The constructed vectors were transfected into the LUAD cell lines using Lipofectamine 2000. Cell viability was detected by MTT, cell migration and invasion were detected by transwell assay, and cell apoptosis was detected by flow cytometry. Western blot was performed to detect the expression levels of the proteins related to the Wnt/ß-catenin pathway and cell apoptosis. Xenograft tumor mouse models were used for in vivo validation. Results: The results of qRT-PCR showed that miR-490-3p was relatively lowly expressed in LUAD cells, and the expression level was different in different LUAD cell lines. The results of MTT, transwell and flow cytometry exhibited that miR-490-3p could significantly inhibit the proliferation, migration, invasion and increase cell apoptosis rate of LUAD cells. Western blot results showed that miR-490-3p promoted the expression of Bax, Caspase-3 and E-cadherin as well as the phosphorylation of GSK-3ß and inhibited the expression of Bcl-2, ß-catenin and C-myc. Additionally, animal experiments were performed to prove that miR-490-3p suppressed LUAD malignant progression in vivo. Conclusion: MiR-490-3p inhibited the proliferation, migration, invasion and promoted the apoptosis of LUAD cells by down-regulating the Wnt/ß-catenin signaling pathway, suggesting that miR-490-3p may be an indicator for early diagnosis and prognosis of LUAD.

18.
Cancer Manag Res ; 12: 11953-11964, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33244271

RESUMO

Purpose: Gastric cancer (GC) is aggressive cancer with a high mortality rate worldwide. N6-methyladenosine (m6A) RNA methylation is related to tumorigenesis, which is dynamically regulated by m6A modulators ("writer," "eraser," and "reader"). We conducted a comprehensive analysis of the m6A genes of GC patients in TCGA datasets to identify the potential diagnostic biomarkers. Materials and Methods: We analyzed the expression profile of m6A genes in the TCGA cohort and constructed a diagnostic-m6A-score (DMS) by the LASSO-logistic model. In addition, by consensus cluster analysis, we identified two different subgroups of GC risk individuals by the expression profile of m6A modulators, revealing that YTHDF1's expression variation profile in GC diagnosis. We also performed RT-qPCR and WB verification in 17 pairs of GC specimens and paired adjacent non-tumor tissues and GC cell lines, and verified the expression trend of YTHDF1 in five GEO GC datasets. YTHDF1 expression and clinical features of GC patients were assessed by the UALCAN. Results: The DMS with high specificity and sensitivity (AUC = 0.986) is proven to distinguish cancer from normal controls better. Moreover, we found that the expression profile variation of YTHDF1 was significantly associated with the high-risk subtype of GC patients. RT-qPCR and Western blot results are consistent with silicon analysis, revealing that YTHDF1's potential oncogene role in GC tumor. Conclusion: In conclusion, we developed the m6A gene-based diagnostic signature for GC and found that YTHDF1 was significantly correlated with the high-risk subtype of GC patients, suggesting that YTHDF1 might be a potential target in GC early diagnosis.

19.
J Orthop Surg Res ; 15(1): 537, 2020 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-33203406

RESUMO

BACKGROUND: To evaluate the biomechanical properties of short-segment Schanz screw implanted in an oblique downward direction for the treatment of lumbar 1 burst fracture using a finite element analysis. METHODS: The Universal Spine System (USS) fixation model for adjacent upper and lower vertebrae (T12 and L2) of lumbar 1 vertebra burst fracture was established. During flexion/extension, lateral bending, and rotation, the screw stress and the displacement of bone defect area of the injured vertebrae were evaluated when the downward inserted angle between the long axis of the screws and superior endplate of the adjacent vertebrae was set to 0° (group A), 5° (group B), 10° (group C), and 15°(group D). There were 6 models in each group. RESULTS: There were no significant differences in the maximum screw stress among all the groups during flexion/extension, lateral bending, and rotation (P > 0.05). There were no significant differences in the maximum displacement of the bone defect area of the injured vertebrae among all the groups during flexion/extension, lateral bending, and rotation (P > 0.05). CONCLUSION: Short-segment Schanz screw implanted in an oblique downward direction with different angles (0°/parellel, 5°, 10°, and 15°) did not change the maximum stress of the screws, and there was a lower risk of screw breakage in all groups during flexion/extension, lateral bending, and rotation. In addition, the displacement of the injured vertebra defect area had no significant changes with the change of angles.

20.
Artigo em Inglês | MEDLINE | ID: mdl-33241690

RESUMO

Metal-organic frameworks (MOFs) are promising materials for gas separation membranes. However, the framework flexibility affects their molecular-sieving properties. Herein, we restrict the flexibility of zeolitic imidazolate framework-7 (ZIF-7) by controlling its phase transition in mixed-matrix membranes (MMMs), relying on the so-called "space-confinement effect" of a novel thermosetting polymer, poly 2,2'-(p-oxydiphenyl)-5,5'-bibenzimidazole (OPBI) polymer. Compared with the pure OPBI membrane, the optimized membranes containing 30 wt % ZIF-7 with a narrow-pore (np) phase (ZIF-7-II) exhibited a significant improvement in H2/CO2 separation, e.g., the H2/CO2 ideal selectivity increased ∼2.8 times, surpassing the state-of-the-art upper bound of polymeric membranes and exhibited excellent stability at increased pressure and temperature (8 bar, 180 °C).

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