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2.
Mol Plant ; 2020 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-32068158

RESUMO

Temperature is a key factor governing the growth and development, distribution, and seasonal behavior of plants. The entire plant lifecycle is affected by environmental temperatures. Plants grow rapidly and exhibit specific changes in morphology under mild average temperature conditions, a response termed thermomorphogenesis. When exposed to chilling or moist chilling low temperatures, flowering or seed germination is accelerated in some plants; these processes are known as vernalization and cold stratification, respectively. After some plants are exposed to chilling temperatures, they acquire the ability to resist freezing stress, a process termed cold acclimation. In the face of global climate change, heat stress is a frequent challenge that adversely affects plant growth and development. In this review, we discuss recent progress in exploring thermomorphogenesis, vernalization, and the mechanisms underlying plant tolerance to extreme temperatures. We also discuss remaining issues that are crucial for understanding the interactions between plants and temperature.

3.
J Integr Plant Biol ; 2020 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-32068336

RESUMO

A recent paper by Kidokoro et al. (2020) in The Plant Cell reported a transgene-dependent transcriptional silencing phenomenon in the dominant ice1-1 Arabidopsis mutant containing the CBF3-LUC reporter, and questioned whether ICE1 may regulate CBF genes and may be involved in plant cold response. Here, we evaluate available evidences supporting the involvement of ICE1 in plant cold response, and provide ChIP-seq data showing ICE1 binding to the promoters of CBF genes and other regulatory genes known to be critical for cold response as well as to the promoters of some COR genes. This article is protected by copyright. All rights reserved.

4.
Plant Cell ; 2020 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-31919300

RESUMO

The phytohormone abscisic acid (ABA) and the Polycomb group (PcG) proteins have key roles in regulating plant growth and development; however, their interplay and underlying mechanisms are not fully understood. Here, we identified an Arabidopsis thaliana nodulin homeobox (AtNDX) protein as a negative regulator in the ABA signaling pathway. AtNDX mutants are hypersensitive to ABA, as measured by inhibition of seed germination and root growth, and the expression of AtNDX is downregulated by ABA. AtNDX interacts with the Polycomb Repressive Complex 1 (PRC1) core components AtRING1A and AtRING1B in vitro and in vivo, and together, they negatively regulate the expression levels of some ABA-responsive genes. We identified ABA-INSENSITIVE (ABI4) as a direct target of AtNDX. AtNDX directly binds the downstream region of ABI4 and deleting this region increases the ABA sensitivity of primary root growth. Furthermore, ABI4 mutations rescue the ABA-hypersensitive phenotypes of ndx mutants and ABI4-overexpressing plants were hypersensitive to ABA in primary root growth. Thus, our work reveals the critical functions of AtNDX and PRC1 in ABA signaling and their regulation of ABI4.

5.
Food Chem Toxicol ; 137: 111139, 2020 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-31981684

RESUMO

We investigated the protective effect and mechanism of selenium-enriched yeast (SY) on caecal injury induced by ochratoxin A (OTA) in broilers. Eighty broiler chickens of 1-day-old with similar weight were randomly assigned to Control group, OTA group, SY group and OTA + SY group, and were intragastricaly administered with OTA and SY for 21 consecutive days. The results showed that SY could reduce the caecal pathological injuries and could inhibit oxidative stress caused by OTA exposure. The OTA + SY group showed a statistically significant (p < 0.01) reduction in the level of MDA, IL-1ß, IL-6 and IFN-γ, whereas the levels of GSH, SOD activity and IL-10 were significantly increased (p < 0.01). By regulating TLR4/MYD88 signaling pathway, SY inhibited the expression of NF-κB, increased the expression of tight junction-related genes Claudin-1, Occludin and ZO-1, and antagonized the intestinal barrier injury caused by OTA exposure. Moreover, the microbial diversity analyses indicated that SY could intervene changes in the diversity of gut microbiota and the imbalance of gut microbiota caused by OTA. SY could relieve caecal pathological injuries, alleviate OTA-induced caecal oxidative stress and inflammatory response, increase the gut microbial diversity and protect broiler's intestinal barrier from injury.

6.
Comput Methods Programs Biomed ; 189: 105352, 2020 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-31991316

RESUMO

BACKGROUND AND OBJECTIVE: To compare the biomechanical changes of adjacent segment degeneration (ASD) after one- or two-level anterior cervical discectomy and fusion (ACDF) versus cervical disc arthroplasty (CDA). METHODS: A three-dimensional finite element (FE) model of intact C2-C7 segments was constructed and validated. In the one-level surgery model, the cage with plate implant or Prestige LP cervical disc prosthesis were integrated at C5-C6 segment into the FE model; while in the two-level surgery model, the prostheses were integrated at both C4-C5 and C5-C6 segments into the FE model. A pure moment of 1.0 Nm combined with a follower load of 73.6 N were imposed on C2 to investigate the flexion-extension, lateral bending, and axial rotation of different segments in the FE model. The segmental range of motion (ROM) and intradiscal pressure of the surgery models were investigated and compared with the intact model. RESULTS: In the one-level model of ACDF, the ROM at C5-C6 was decreased, the ROM and intradiscal pressure at C4-C5 and C6-C7 segments were increased. In the two-level model of ACDF, the ROM at C4-C5 and C5-C6 were decreased, the ROM and intradiscal pressure at C3-C4 and C6-C7 were increased. However, in both one- and two-level models of CDA, the ROM of surgery segments were preserved, avoiding the increase of the ROM and intradiscal pressure at the adjacent segments. CONCLUSIONS: Abnormal ROM and intradiscal pressure at the adjacent segments may contribute to the higher risk of ASD after ACDF compared with CDA.

7.
J Cell Biochem ; 2020 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-31898343

RESUMO

Long noncoding RNAs (lncRNAs) were identified as a vital part in the development and progression of cancer in recent years. Colorectal neoplasia differentially expressed (CRNDE), a lncRNA, functions as an oncogene in some malignant neoplasias, but its role in the progression of osteosarcoma (OS) is still poorly understood. To dissect the difference in the expression of CRNDE, quantitative real-time polymerase chain reaction was utilized to evaluate it in OS tissues and cell lines (U2OS, MG63, and MNNG/HOS) compared with that in the adjacent normal tissues/osteoblast cells (hFOB1.19). The role of CRNDE in OS lines was assessed using Cell Counting Kit-8, colony formation, 5-ethynyl-2'-deoxyuridine staining, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling staining, flow cytometry, Transwell assays, and Western blot, respectively. The results demonstrated that the expression of CRNDE was high in OS tissues and cell lines, and partly induced by SP1. CRNDE knockdown attenuated OS cell proliferation and invasion and induced apoptosis and G0/G1 arrest. Moreover, the expression of mesenchymal markers N-cadherin, Vimentin and Snail were downregulated, while the expression of epithelial markers E-cadherin and ZO-1 were conversely upregulated due to CRNDE knockdown. The mechanistic investigations showed that CRNDE promoted glycogen synthase kinase-3ß phosphorylation to activate the Wnt/ß-catenin pathway. The results suggested that lncRNA CRNDE indeed contributed to OS proliferation, invasion, and epithelial-mesenchymal transition, working as an oncogene, demonstrating that lncRNA CRNDE may be a valid therapeutic target for the OS.

8.
Bioresour Technol ; 301: 122739, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31945683

RESUMO

Fast pyrolysis of corn stalk (CS) was performed by pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) and the product distribution was measured as a function of temperature, time, and catalyst. The types and yields of product compounds were influenced dramatically by temperature, while the duration of the reaction had little effect on the type of compound. Three primary components in the biomass interacted during pyrolysis. The maximum proportions of aldehydes (27.26%), furans (5.93%), and olefins (6.46%), and the minimum proportions of alcohols (0%) and carbohydrates (0.74%) were obtained over MCM-41. HZSM-5 improved the selectivity of aromatic hydrocarbons while inhibiting acid formation. The proportion of N-compounds was maximal (23.39%) over ZrO2. ZnCl2 tended to generate the least amounts of ketones (2.02%), phenols (9.08%), and esters (2.16%), but the greatest amount of carbohydrates (37.31%). K2SO4 promoted the formation of acids, ketones, alcohols, and phenols, while reducing the production of N-compounds and aldehydes.

9.
J Cell Physiol ; 235(3): 2911-2924, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31535374

RESUMO

Pyroptosis is a form of programmed cell death (PCD) that plays a vital role in immunity and diseases. Although it was recently reported that chemotherapy drugs can induce pyroptosis through caspase-3-dependent cleavage of gasdermin E (GSDME), the role of pyroptosis in osteosarcoma (OS) with dioscin is less understood. In this study, we explored the effects of dioscin on OS in vitro and in vivo and further elucidated the underlying molecular mechanisms and found that dioscin-triggered pyroptosis in GSDME-dependent cell death and that GSDME-N was generated by caspase-3. Furthermore, dioscin inhibited cancer cell growth by inducing G2/M arrest and apoptosis through the JNK/p38 pathway. In vivo, dioscin significantly inhibited OS proliferation. Taken together, our results demonstrate that dioscin can induce apoptosis through the JNK/p38 pathway and GSDME-dependent pyroptosis in OS, identifying it as a potential therapeutic drug for treatment of this disease.

10.
Biochim Biophys Acta Mol Basis Dis ; 1866(1): 165575, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31666206

RESUMO

Intervertebral disc degeneration (IVDD) is a well-known cause of lower back pain. Icariin has been shown to exert a protective effect on human nucleus pulposus (NP) cells and accordingly has implications for the prevention and treatment of IVDD; however, the molecular mechanisms underlying its action are not fully established. In this study, the mechanisms underlying its protection against hydrogen peroxide (H2O2)-induced oxidative stress injury were investigated. In vitro, we demonstrated that icariin inhibits H2O2-induced mitochondria-mediated apoptosis. It upregulates oxidative stress mediators, i.e., reactive oxygen species, and downregulates mitochondrial membrane potential. Nuclear factor erythroid 2-related factor 2 (Nrf-2) is a key factor involved in the regulation of the endogenous defense system. It was proved that icariin could activate the Nrf-2 signaling pathway, upregulate the protein expression of nuclear respiratory factor-1 and the mitochondrial transcription factor, promoting mitochondrial biogenesis in human NP cells. An Nrf-2 agonist and inhibitor promoted or partly abolished the protective effects of icariin on mitochondrial homeostasis. Moreover, it was demonstrated that the Nrf-2 signaling pathway could be inhibited by the phosphatidylinositol 3-Kinase/AKT pathway. In vivo, icariin ameliorated IVDD in a rat model by promoting Nrf-2 activity, and preserving extracellular matrix in NP cells. These data suggest that icariin could ameliorate IVDD in rat models in vivo. In summary, the protective effects of icariin on human NP cells may suppress the pathogenesis of IVDD via the Nrf-2 signaling pathway. Our findings suggest that the Nrf-2 signaling pathway is a novel therapeutic target for the treatment of IVDD.

11.
Spine (Phila Pa 1976) ; 44(24): 1697-1704, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31794507

RESUMO

MINI: The surgical results of modified and traditional open-door laminoplasties were retrospectively compared. There were no differences in neurological outcomes and postoperative complications. However, compared with traditional laminoplasty, modified laminoplasty was associated with better maintenance of cervical curvature and ROM, as well as a lower incidence of postoperative axial symptoms. STUDY DESIGN: A retrospective comparative study. OBJECTIVE: The aim of this study was to evaluate the efficacy of modified unilateral open-door laminoplasty with posterior muscle-ligament complex preservation in treating cervical spondylotic myelopathy (CSM). SUMMARY OF BACKGROUND DATA: The prevalence of axial symptoms after open-door laminoplasty ranges from 5% to 86%. Current modified laminoplasty techniques cannot satisfactorily reduce postoperative axial symptoms. METHODS: From June 2014 to July 2016, 36 patients with CSM underwent modified laminoplasty with posterior muscle-ligament complex preservation (modified group) and 27 patients underwent traditional laminoplasty (control group). The demographics, operation duration, blood loss volume, cervical curvature, cervical curvature index (CCI), range of motion (ROM), cervical posterior muscle volume on magnetic resonance imaging (MRI), axial symptoms, visual analog scale (VAS) score, Japanese Orthopedic Association (JOA) score, neck disability index (NDI), and complications were recorded and analyzed before operation, after operation, and at the final follow-up. RESULTS: Cervical flexion curvature, cervical curvature, ROM, and CCI were better in the modified group than in controls at the final follow-up (P < 0.01). Postoperative VAS and NDI scores were significantly reduced in the modified group compared with controls (1.15 ±â€Š0.76 vs. 2.63 ±â€Š1.06, 5.67 ±â€Š2.57 vs. 8.89 ±â€Š5.37, respectively) (P < 0.01). Posterior muscle volume at the hinge side in the modified group was not different at final follow-up relative to that before the operation, whereas it was reduced in controls (P < 0.01). Finally, the incidence of axial symptoms was significantly lower in the modified group than in controls (6% vs. 22%) (P = 0.03). CONCLUSION: Modified unilateral open-door laminoplasty with posterior muscle-ligament complex preservation is effective for treating CSM with good recovery of neurological functions and satisfactory maintenance of cervical curvature and ROM. Furthermore, it reduces the incidence of postoperative axial symptoms compared with conventional open-door laminoplasty. LEVEL OF EVIDENCE: 3.

12.
Injury ; 2019 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-31839426

RESUMO

PURPOSE: To evaluate intraoperative and early postoperative clinical outcomes using the Nice knot as an auxiliary reduction technique in displaced comminuted patellar fractures. METHODS: Thirty-nine patients with unilateral closed displaced comminuted patellar fractures received open reduction and internal fixation (ORIF), utilizing either Nice knot (the NK group, 24 patients) or traditional reduction (the TR group, 23 patients) techniques, were retrospectively reviewed in this study. Intra-operative surgical time and peri-operative hemoglobin were recorded. Post-operative clinical outcomes were measured using visual analgesic score, range of motion of the knee joint and the Böstman scales, and radiographic outcomes were used to evaluate fracture healing. Complications including infection, bone non-union, implant loosening, fragment displacement and painful hardware were also assessed. RESULTS: In-hospital records indicated significantly shorter surgical duration (32.6 min) in the NK group than in the TR group (63.9 min). Intraoperative blood loss was also significantly decreased in the NK group (64.7 ml) compared to the TR group (189.1 ml). Patients in the NK and TR groups were followed for mean of 12.9 months and 12.5 months respectively. The union rate was 100% (24/24) in the NK group and 91.3% (21/23) in the TR group. In the TR group, there were two non-unions, including one infected non-union. There was no difference in the visual analgesic score, the range of motion of the knee joint or the Böstman scale at last follow-up between the two groups. CONCLUSION: The sliding, self-stabilizing Nice knot was associated with reduced surgical time, decreased intraoperative blood loss, and satisfactory postoperative outcomes in the treatment of displaced patellar fractures. Future studies are needed to ensure the generalizability of these findings to additional patient populations at other institutions.

13.
Eur Spine J ; 2019 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-31834484

RESUMO

PURPOSE: No standard strategy exists for managing cervical spondylotic myelopathy (CSM). The efficacy of spinous process-splitting laminoplasty, its impact on cervical alignment change and the incidence of postoperative neck pain remain unclear. We analyzed the parameters of cervical alignment and cord morphology in CSM. METHODS: The radiographic parameters investigated were pre- and postoperative C2-C7 lordosis (CL), C2-C7 sagittal vertical axis (CSVA), T1 slope (TS), TS minus CL (TS - CL) and cervical spinal cord morphology. Myelopathy severity was measured using two different functional scores. Statistical analysis was performed to determine significant differences between preoperative and follow-up radiological findings and change in functional scores. RESULTS: This retrospective study comprised 85 CSM patients from a single institute, with a minimum follow-up of 24 months. Overall, 63.5% (n = 54) of patients had improvement in their postoperative cervical lordotic alignment; 36.5% (n = 31) developed progressive aggravation of the cervical kyphotic alignment. Pearson correlation analysis showed that CSVA, TS and T1-CL were independent predictors of CL curve change. Based on the receiver operating characteristic curve, the cutoff value for CSVA was 2.89 cm with a postoperative visual analog scale (VAS) > 4. The cutoff value of the TS - CL was 20 degrees with a postoperative VAS > 4. CSVA, TS and TS - CL had a significant association with variation in CL. CSVA and TS - CL had a significant association with postoperative neck pain. CONCLUSIONS: CSVA, T1 slope and T1-CL are good predictors of postoperative degenerative kyphotic change and neck pain. Careful consideration of their preoperative cutoff values can improve postoperative outcomes. LEVEL OF EVIDENCE: IV. These slides can be retrieved under Electronic Supplementary Material.

14.
Sensors (Basel) ; 19(22)2019 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-31744093

RESUMO

Rotating stall limits the operating range and stability of the centrifugal compressor and has a significant impact on the lifetime of the impeller blade. This paper investigates the relationship between stall pressure wave and its induced non-synchronous blade vibration, which will be meaningful for stall resonance avoidance at the early design phase. A rotating disc under a time-space varying load condition is first modeled to understand the physics behind stall-induced vibration. Then, experimental work is conducted to verify the model and reveal the mechanism of stall cells evolution process within flow passage and how blade vibrates when suffering such aerodynamic load. The casing mounted pressure sensors are used to capture the low-frequency pressure wave. Strain gauges and tip timing sensors are utilized to monitor the blade vibration. Based on circumferentially distributed pressure sensors and stall parameters identification method, a five stall cells mode is found in this compressor test rig and successfully correlates with the blade non-synchronous vibration. Furthermore, with the help of tip timing measurement, all blades vibration is also evaluated under different operating mass flow rate. Analysis results verify that the proposed model can show the blade forced vibration under stall flow condition. The overall approach presented in this paper is also important for stall vibration and resonance free design with effective experimental verification.

15.
Proc Inst Mech Eng H ; 233(12): 1310-1317, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31617820

RESUMO

This study aimed to evaluate the safety and accuracy of mixed reality-based intraoperative three-dimensional navigated pedicle screws in three-dimensional printed model of fractured upper cervical spine. A total of 27 cervical model from patients of upper cervical spine fractures formed the study group. All the C1 and C2 pedicle screws were inserted under mixed reality-based intraoperative three-dimensional image-guided navigation system. The accuracy and safety of the pedicle screw placement were evaluated on the basis of postoperative computerized tomography scans. A total of 108 pedicle screws were properly inserted into the cervical three-dimensional models under mixed reality-based navigation, including 54 C1 pedicle screws and 54 C2 pedicle screws. Analysis of the dimensional parameters of each pedicle at C1/C2 level showed no statistically significant differences in the ideal and the actual entry points, inclined angles, and tailed angles. No screw was misplaced outside the pedicle of the three-dimensional printed model, and no ionizing X-ray radiation was used during screw placement under navigation. It is easy and safe to place C1/C2 pedicle screws under MR surgical navigation. Mixed reality-based navigation is feasible within upper cervical spinal fractures with improved safety and accuracy of C1/C2 pedicle screw insertion.

16.
Environ Sci Pollut Res Int ; 26(33): 34575-34583, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31650475

RESUMO

The aim of the present study was to investigate whether curcumin (CUR) can ameliorate cadmium-induced reproductive toxicity and its mechanism. A total of 48 male mice were equally divided into 4 groups: control, CdCl2 (2 mg/kg, intraperitoneally inject) curcumin (50 mg/kg, intraperitoneally inject), co-treatment with curcumin (50 mg/kg), and CdCl2 (2 mg/kg) for 10 days. The results demonstrated that CdCl2 reduces sperm motility, decreases the sperm density and serum testosterone content, and significantly improves the rate of sperm deformity. CdCl2 increased the level of testicular total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) activity, and glutathione (GSH), and CdCl2 declined the level of malondialdehyde (MDA). However, the semen quality of the mice in the curcumin intervention group was improved. Moreover, the testosterone content and antioxidant capacity were increased. In the Cd group mice, the expression of testicular Nrf2, as well as the mRNA and protein expressions of the downstream target molecules, glutathione peroxidase (GSH-Px), and γ-glutamylcysteine synthetase (γ-GCS) of Nrf2 declined, while the above genetic expressions elevated significantly in the curcumin intervention group. Our results suggested that curcumin could protect against Cd-induced testicular injury via activating the Nrf2/ARE signaling pathway.


Assuntos
Antioxidantes/farmacologia , Cádmio/toxicidade , Curcumina/farmacologia , Testículo/fisiologia , Animais , Antioxidantes/metabolismo , Fator de Transcrição de Proteínas de Ligação GA/metabolismo , Glutationa/metabolismo , Glutationa Peroxidase/metabolismo , Masculino , Malondialdeído/metabolismo , Camundongos , Fator 2 Relacionado a NF-E2/metabolismo , Oxirredução , Estresse Oxidativo/efeitos dos fármacos , Substâncias Protetoras/farmacologia , Análise do Sêmen , Transdução de Sinais/efeitos dos fármacos , Motilidade Espermática/efeitos dos fármacos , Superóxido Dismutase/metabolismo , Testículo/efeitos dos fármacos , Testículo/metabolismo , Testosterona/metabolismo
17.
Emerg Infect Dis ; 25(11): 2021-2030, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31600132

RESUMO

Invasive group B Streptococcus (GBS) remains a leading cause of illness and death among infants globally. We conducted prospective and retrospective laboratory-based surveillance of GBS-positive cultures from infants <3 months of age in 18 hospitals across China during January 1, 2015-December 31, 2017. The overall incidence of GBS was 0.31 (95% CI 0.27-0.36) cases/1,000 live births; incidence was 0-0.76 cases/1,000 live births across participating hospitals. The case-fatality rate was 2.3%. We estimated 13,604 cases of GBS and 1,142 GBS-associated deaths in infants <90 days of age annually in China. GBS isolates were most commonly serotype III (61.5%) and clonal complex 17 (40.6%). Enhanced active surveillance and implementation of preventive strategies, such as maternal GBS vaccination, warrants further investigation in China to help prevent these infections.

18.
Exp Ther Med ; 18(4): 2933-2941, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31572536

RESUMO

Traumatic soft tissue defects such as bedsores, chronic skin ulcers, limb necrosis, osteonecrosis and other ischemic orthopedic diseases are the most clinically intractable and common problems in orthopedics due to unsatisfactory conventional treatments. The present study designed poly(ethylene glycol; PEG) hydrogels with covalently binded arginylglycylaspartic acid (RGD). Endothelial progenitor cells (EPCs) were encapsulated in the modified hydrogel along with vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF). Results demonstrated that the modified hydrogel displayed good mechanical properties appropriate for a sustained release carrier. RGD modification significantly promoted EPC biocompatibility. VEGF and bFGF encapsulation enhanced the adhesion of EPCs, promoted the production of extracellular matrix and facilitated EPC proliferation. In addition, bFGF and VEGF induced angiogenesis. The combination of growth factors and EPCs in the hydrogel displayed a strong synergy to improve biocompatibility. The present results provided a potential novel treatment approach for soft tissue defects such as bone exposure, chronic skin ulcers, bedsores, limb necrosis, osteonecrosis and other ischemic diseases.

19.
Artigo em Inglês | MEDLINE | ID: mdl-31639109

RESUMO

OBJECTIVES: This aim of this project was to promote best practice in fluid balance monitoring in congestive heart failure patients. INTRODUCTION: Fluid overload or pulmonary/vascular congestion is a common clinical feature of patients with heart failure and is associated with adverse outcomes. Maintaining records of patients' fluid intake and output (I&O) has long been considered an important aspect of nursing care to assess hydration status. In clinical practice, a prevalence of incongruence between I&O results and clinical signs and symptoms has been noted. We postulate that this incongruence may be due to an inaccurate or non-comprehensive collection of fluid I&O and/or the patient's and their families' inadequate recognition of the importance of I&O recording. METHODS: This implementation project was conducted in a cardiology ward of a medical hospital using the Joanna Briggs Institute Practical Application of Clinical Evidence System. Three audit criteria were utilized to represent best practice recommendations for fluid balance monitoring. A pre-implementation audit was conducted, followed by development of multiple interventions identified from a Getting Research into Practice analysis. Two post-implementation audits were conducted to determine the change in practice. RESULTS: The post-implementation audit data demonstrated significantly improved outcomes across the measured best practice criteria. Specifically, improvement in compliance was noted in: i) fluid balance charts being utilized in conjunction with physical assessment and electrolyte monitoring to assess the hydration status of a patient (from 58% to 100% compliance); ii) patient involvement in more accurate documentation (from 42% to 75% compliance); and iii) staff education in fluid balance monitoring and documentation (from 53% to 100% compliance). CONCLUSION: Fluid record omission was reduced at the completion of the project secondary to an increased awareness of the fluid record omission standards among patients as well as physical assessment and fluid overload correlation among the nursing staff in the unit. The findings demonstrate how audits can be utilized to promote best practice in healthcare. Additionally, the data indicates that focused education and the provision of relevant resources can have an immediate and positive impact on clinical practice.

20.
Exp Mol Med ; 51(9): 110, 2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31554783

RESUMO

Intervertebral disc degeneration (IDD) is characterized by excessive apoptosis of nucleus pulposus (NP) cells and hyperactive extracellular matrix (ECM) catabolism. Our previous studies revealed the relationship between human islet amyloid polypeptide (hIAPP) and NP cell apoptosis. However, the role of hIAPP aggregates in IDD has not yet been investigated. This study aimed to determine whether the accumulation of hIAPP aggregates promotes IDD progression. The aggregation of hIAPP increased in human NP tissues during IDD. The deposition of hIAPP aggravated the compression-induced IDD that promoted NP cell apoptosis and ECM degradation via IL-1ß/IL-1Ra signaling in an ex vivo rat disc model. Moreover, neutralizing IL-1ß augmented the protective effects of hIAPP overexpression by decreasing hIAPP aggregation in human NP cells. These results suggest that the aggregation of hIAPP promotes NP cell apoptosis and ECM degradation ex vivo and in vitro by disrupting the balance of IL-1ß/IL-1Ra signaling.

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