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1.
Methods Mol Biol ; 2221: 165-191, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-32979204

RESUMO

Our laboratories have used genetically engineered mouse models (GEMMs) to assess genetic contributions to skeletal diseases such as osteoporosis and osteoarthritis. Studies on the genetic contributions to OA are often done by assessing how GEMMs respond to surgical methods that induce symptoms modeling OA. Here, we will describe protocols outlining the induction of experimental OA in mice as well as detailed descriptions of methods for analyzing skeletal phenotypes using micro-computerized tomography and skeletal histomorphometry.

2.
J Phys Chem A ; 2020 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-33045834

RESUMO

Traditionally, chemistry problems are solved by means of a deductive approach. The question to be addressed is typically related to the value of a property that is either measured experimentally, computed using quantum-chemistry software, or (more recently) predicted using a machine-learned model. In this paper, we demonstrate that an inductive approach can be adopted using End-to-End (E2E) machine learning. This approach is illustrated for tackling the following chemistry problems: (i) determine the fully coordinated (FC) and undercoordinated (UC) atoms in a molecule with one missing atom, (ii) identify the type of atom that is missing in such an incomplete molecule, and (iii) predict the direction of a reaction between two molecules according to an existing dataset. The E2E approach leads to accuracies higher than 99%, 98%, and 93% for these three problems, respectively. Finally, in order to achieve such accuracies, a descriptor for the molecules, called bag of clusters, is introduced and compared with a series previously proposed descriptors, highlighting a series of advantages.

3.
Transpl Infect Dis ; : e13488, 2020 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-33034139

RESUMO

We describe the first published case of malakoplakia in a dual stem-cell and cardiac transplant recipient. In the two months following cardiac transplantation, our patient developed persistent diarrhea and recurrent E. coli bacteraemia. Biopsies obtained from areas of colonic thickening revealed malakoplakia. Despite improvement in symptoms with prolonged antimicrobial therapy and reduction of his immunosuppression, he eventually died from sepsis. Our case highlights not only the importance of the timely diagnosis of this rare disorder, but also the difficulty in determining optimal treatment duration, particularly where excision of involved areas is not possible, as data on this disease are lacking. Here we describe our case and review the available literature published on malakoplakia in the cardiac transplant population.

4.
J Orthop Surg (Hong Kong) ; 28(3): 2309499020945118, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33034241

RESUMO

PURPOSE: The treatment for young patients with advanced knee arthritis by Oxford unicompartmental knee arthroplasty (Oxford-UKA) is still controversial. A relative few studies were published about Asian patients. We evaluated midterm results of Oxford-UKA in young patients aged less than or equal to 60 years. METHODS: Between December 2009 and December 2018, 95 patients (109 knees) (mean age 57.48 years; range: 33-60 years) were included. The mean follow-up period was 3.0 years (range: 1.0-9.5 years). The functional outcomes and pain score of the knee before and after surgery were examined. RESULTS: To the last follow-up, no patient died, six patients (nine knees) were lost and one patient underwent total knee revision in case of unexplained postoperative pain. The patients demonstrated significant improvement in range of knee motion (103.65 vs. 115.96, p < 0.05), Oxford Knee Score (40.0 vs. 14.4, p < 0.05), Hospital for Special Surgery Score (56.0 vs. 85.7, p < 0.05), and Visual Analogue Score (VAS) (5.23 vs. 1.08, p < 0.05) before and after surgery. CONCLUSION: Our midterm results showed that the Oxford-UKA was a reliable and effective treatment option for young patients aged less than or equal to 60 years with anterior medial osteoarthritis. The knee pain can be effectively relieved and excellent functional activities of the knee joint can be obtained after surgery.

5.
Artigo em Inglês | MEDLINE | ID: mdl-33014885

RESUMO

Neisseria gonorrhoeae, a Gram-negative obligate human pathogenic bacterium, infects human epithelial cells and causes sexually transmitted diseases. Emerging multi-antibiotic resistant gonococci and increasing numbers of infections complicate the treatment of infected patients. Here, we used an shRNA library screen and next-generation sequencing to identify factors involved in epithelial cell infection. Folliculin (FLCN), a 64 kDa protein with a tumor repressor function was identified as a novel host factor important for N. gonorrhoeae survival after uptake. We further determined that FLCN did not affect N. gonorrhoeae adherence and invasion but was essential for its survival in the cells by modulating autophagy. In addition, FLCN was also required to maintain cell to cell contacts in the epithelial layer. In an infection model with polarized cells, FLCN inhibited the polarized localization of E-cadherin and the transcytosis of gonococci across polarized epithelial cells. In conclusion, we demonstrate here the connection between FLCN and bacterial infection and in particular the role of FLCN in the intracellular survival and transcytosis of gonococci across polarized epithelial cell layers.

6.
Annu Int Conf IEEE Eng Med Biol Soc ; 2020: 3019-3022, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33018641

RESUMO

Steady-State Visual Evoked Potentials (SSVEP) Brain-Computer Interface (BCI) relies on overt spatial attention to exhibit reliable steady-state responses. There is a promising potential to employ the SSVEP paradigm in with vision research and clinical use, for instance, for visual field assessment. In this study, we investigate the SSVEP characteristics with different spatial attention, the different number of stimuli, and different viewing/visual angles. We collected data from eleven subjects in three experiment sessions, lasting about forty minutes, including the setup and calibration. Our evaluation results show similar SSVEP responses between overt and covert attention in multiple stimuli scenarios in most of the visual angles. We do not find any significant differences in SSVEP responses in visual angles between single and multi stimuli in covert attention. From this study, we found that reliable SSVEP responses can be achieved with covert spatial attention regardless of visual angles and stimulus spatial resolution.

7.
Annu Int Conf IEEE Eng Med Biol Soc ; 2020: 1307-1310, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-33018228

RESUMO

This paper presents a new 3D CT image reconstruction for limited angle C-arm cone-beam CT imaging system based on total-variation (TV) regularized in image domain and L1-penalty in projection domain. This is motivated by the facts that the CT images are sparse in TV setting and their projections are sinusoid-like forms, which are sparse in the discrete cosine transform (DCT) domain. Furthermore, the artifacts in image domain are directional due to limited angle views, so the anisotropic TV is employed. And the reweighted L1penalty in projection domain is adopted to enhance sparsity. Hence, this paper applied the anisotropic TV-norm and reweighted L1-norm sparse techniques to the limited angle Carm CT imaging system to enhance the image quality in both CT image and projection domains. Experimental results also show the efficiency of the proposed method.Clinical Relevance-This new CT reconstruction approach provides high quality images and projections for practicing clinicians.

8.
Annu Int Conf IEEE Eng Med Biol Soc ; 2020: 1311-1314, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-33018229

RESUMO

Metal Artifact Reduction (MAR) plays an important role in Computed Tomography (CT) research and application because severe artifacts degrade the image quality and diagnosis value if metal objects are present in the field of measurement. Although there are already many works for MAR, these works are for fan beam CT, not for cone beam CT, which is the trend and receiving much research attention. In this paper, we extend the Normalized Metal Artifact Reduction (NMAR) for fan beam CT to NMAR3 for cone beam CT, by replacing the linear interpolation in the NMAR with bi-linear interpolation. Experiments are carried out on 17 sets of spine phantom CT. 15 of them have reference CT as ground truth and 2 ones not. Both quantitative and qualitative results verified that NMAR3 outperforms the baseline method, i.e., bi-linear interpolation based method.

9.
Annu Int Conf IEEE Eng Med Biol Soc ; 2020: 1572-1575, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-33018293

RESUMO

The limited-angle cone-beam Computed Tomography (CT) is often used in C-arm for clinical diagnosis with the advantages of cheap cost and radiation dose reduction. However, due to incomplete projection data, the 3-dimensional CT images reconstructed by conventional methods, such as the Feldkamp, Davis and Kres (FDK) algorithm [1], suffer from heavy artifacts and missing features. In this paper, we propose a novel pipeline of neural networks jointly by a FDK-based neural network revisited from Würfl et al.'s work [2] and an image domain U-Net to enhance the 3-dimensional reconstruction quality for limited projection sinogram less than 180 degrees, i.e. 145 degrees in our work. Experimental results, on simulated projections of real-scan CTs, show that the proposed pipeline can reduce some of the major artifacts caused by the limited views while keep the key features, with a 16.60% improvement than Würfl et al.'s work on peak signal-to-noise ratio.

10.
Environ Pollut ; 268(Pt B): 115676, 2020 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-33038572

RESUMO

Little is known about interactive effects of pH-aluminum (Al) on reactive oxygen species (ROS) and methylglyoxal (MG) metabolisms in plants. Citrus sinensis seedlings were fertilized with nutrient solution at an Al concentration of 1 or 0 mM and a pH of 4.0, 3.5, 3.0 or 2.5 for 18 weeks. Thereafter, gas exchange and chlorophylls in leaves, H2O2 generation, electrolyte leakage, total soluble proteins, MG, malondialdehyde (MDA), antioxidants, sulfur-containing compounds, enzymes [viz., antioxidant enzymes, sulfur metabolism-related enzymes, ascorbate oxidase, phosphomannose isomerase, glyoxalase I and glyoxalase II] involved in ROS and MG detoxification in leaves and roots were measured. Effects of low pH and Al-toxicity on these parameters displayed obvious synergism. Without Al-toxicity, low pH increased H2O2 production, electrolyte leakage, MDA and MG concentrations by 45.7%-90.3% (52.4%-73.6%), 24.3%-74.5% (26.7%-86.2%), 18.6%-44.8% (35.6%-53.7%) and 16.3%-47.1% (13.8%-51.7%) in leaves (roots) relative to pH 4, respectively; low pH-induced upregulation of enzymes involved in ROS and MG detoxification and sulfur-containing compounds in leaves and/or roots could not protect them against oxidative damage. At pH 2.5-3.0, Al-toxicity increased H2O2 production, electrolyte leakage, MDA and MG concentrations by 34.2%-35.5% (23.9%-72.7%), 10.2%-29.5% (23.7%-56.8%), 15.6%-35.7% (27.5%-33.9%) and 21.5%-26.8% (21.0%-49.2%) in leaves (roots), respectively, and decreased total soluble protein concentration by 46.2%-47.4% (18.8%-20.8%) in leaves (roots); at pH 3.5-4.0, Al-toxicity did not affect significantly the five parameters in leaves and roots except for Al-induced increases in root MDA concentration at pH 3.5-4.0 and root electrolyte leakage at pH 3.5, and Al-induced decrease in root total soluble protein concentration at pH 4.0. Raised pH conferred the ability to maintain a balance between production and detoxification of ROS and MG in leaves and roots, thus protecting them against oxidative damage, and hence alleviating Al-induced increase in electrolyte leakage and decrease in total soluble protein level.

11.
Chemistry ; 2020 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-32902004

RESUMO

Pitch has been used to prepare electrodes by high-temperature heat-treatment for supercapacitors, lithium ion batteries, on account of its rich aromatic ring structure. Here, toluene soluble component of pitch is used to prepare a kind of laminated carbon. It was realized by a template-free synthesis in the low temperature with the addition of pressure. Toluene soluble component has a small molecular weight, it makes the thermal deformation ability stronger and then enhances the orientation of carbon layer with the help of pressure. The prepared anode exhibits a splendid electrochemical performance compared to the traditional graphite anode. A high stable capacity of ca. 550 mAh g -1 at 50 mA g -1 which is higher much than graphite (372 mAh g -1 ) is obtained. And when the current density is up to 2 A g -1 , the capacity is about 150 mAh g -1 . Surprisingly, it also delivers a superior cycling performance. And when used as anode/cathode electrode for lithium ion capacitor, a high energy density can be obtained. The present work offers an opportunity to utilize the pitch source in lithium energy storage with promising cycle life, high energy/power density and low cost.

12.
Updates Surg ; 2020 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-32970309

RESUMO

Recurrence of common bile duct stones (CBDS) is common after surgical stone extraction, but the causes of recurrence are not fully understood. This study aimed to report the experience of treating recurrent CBDS. A total of 106 consecutive cases of recurrent CBDS treated from January 2006 to December 2015 were included. During surgery, the choledochoscopic pass-through test was performed to assess the structure and function of the duodenal papilla. The choledochoscopic pass-through test revealed 62 patients (58.49%) with incomplete closure of the lower end of the common bile duct, and 28 (26.42%) with stenosis at the lower common bile duct. Intra-operative bile bacterial culture was positive in 98 (92.45%) patients. The rate of complete stone clearance was 99.1%. The total recurrence rate of CBDS was 3.13%. The long-term success rate of surgical treatment (excellent and good) reached 92.7% .Duodenal papilla (Oddi sphincter) dysfunction is the main cause of recurrent CBDS. Common bile duct exploration with stone extraction, hilar ductoplasty, and Roux-en-Y hepaticojejunostomy can effectively eliminate the recurrence of CBDS and reduce the incidence of post-hepaticojejunostomy complications. Thus, it is an optimal surgical procedure for recurrent CBDS.

13.
Biochim Biophys Acta Rev Cancer ; 1874(2): 188439, 2020 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-32980465

RESUMO

Over the past decades, tumor-resident immune cells have been extensively studied to dissect their biological functions and clinical roles. Tumor-infiltrating CD8+ T cells, because of their cytotoxic and killing ability, have been under the spotlight for a long time, whereas CD4+ T cells are considered just a supporting actor in the field of cancer immunotherapy. Until recently, accumulating evidence has demonstrated the ability of CD4+ T cells in eradicating solid tumors, and their functions in mediating antitumor immunity have been investigated in various orientations. In this review, we highlight the pivotal role of CD4+ T cells in eliciting vigorous antitumor immune responses, summarize key signaling axes and molecular networks behind these antitumor functions, and also propose possible targets and promising strategies which might translate into more efficient immunotherapies against human cancers.

14.
Mol Neurobiol ; 2020 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-32895785

RESUMO

Alcohol use-associated disorders are highly comorbid with anxiety disorders; however, their mechanism remains unknown. The amygdala plays a central role in anxiety. We recently found that 7,8-dihydroxyflavone (7,8-DHF) significantly reduces withdrawal symptoms in a rat model of chronic intermittent alcohol (ethanol) exposure. This study aimed to determine the role of 7,8-DHF in regulating anxiety induced by chronic alcohol exposure and its associated underlying mechanism. Male C57BL/6J mice were exposed to chronic intermittent alcohol for 3 weeks followed by alcohol withdrawal for 12 h with or without 7,8-DHF administered intraperitoneally. All mice were tested using an open field test and elevated plus maze to assess anxiety-like behaviors. Synaptic activity and intrinsic excitability in basal and lateral amygdala (BLA) neurons were assessed using electrophysiological recordings. 7,8-DHF alleviated alcohol-induced anxiety-like behavior and attenuated alcohol-induced enhancement of activities in BLA pyramidal neurons. Furthermore, 7,8-DHF prevented alcohol withdrawal-evoked augmentation of glutamatergic transmission in the amygdala and had no effect on GABAergic transmission in the amygdala, as demonstrated by unaltered frequency and amplitude of spontaneous inhibitory postsynaptic currents. Microinjection of K252a, a tropomyosin-related kinase B (TrkB) antagonist, into the BLA blocked the effects of 7,8-DHF on anxiety-like behavior and neuronal activity in the BLA. Our findings suggest that 7,8-DHF alleviates alcohol-induced anxiety-like behavior induced by chronic alcohol exposure through regulation of glutamate transmission involving TrKB in the BLA.

15.
BMC Musculoskelet Disord ; 21(1): 585, 2020 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-32867743

RESUMO

BACKGROUND: Secondary osteonecrosis of the knee is a rare event. There are few reports regarding management of this condition. The aim of the present study is to report treatment outcomes for secondary osteonecrosis of the lateral condyle treated with unicompartmental knee arthroplasty (UKA). CASE PRESENTATION: A 54-year-old woman with idiopathic thrombocytopenic purpura, who received low-dosage corticosteroids, complained of knee pain for 5 years and difficulty walking in the last 5 months. Fixed-bearing lateral UKA was performed under general anesthesia combined with midthigh saphenous nerve block. The patient could walk without ambulation aid shortly after the operation and achieved satisfactory knee joint function at the 6-week follow-up. The knee society score (KSS) increased from 68 to 91. The follow-up period was up to 1 year. There was no pain, loosening, or fracture of the prosthesis at the latest follow-up. CONCLUSIONS: This case study demonstrates successful management of secondary osteonecrosis of the lateral femoral condyle is possible with a fixed bearing lateral UKA. Early diagnosis, rigorous indication, and appropriate surgical techniques were critical to maximizing prosthesis stability in lateral UKA.

16.
Cell ; 183(1): 211-227.e20, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-32937106

RESUMO

The striosome compartment within the dorsal striatum has been implicated in reinforcement learning and regulation of motivation, but how striosomal neurons contribute to these functions remains elusive. Here, we show that a genetically identified striosomal population, which expresses the Teashirt family zinc finger 1 (Tshz1) and belongs to the direct pathway, drives negative reinforcement and is essential for aversive learning in mice. Contrasting a "conventional" striosomal direct pathway, the Tshz1 neurons cause aversion, movement suppression, and negative reinforcement once activated, and they receive a distinct set of synaptic inputs. These neurons are predominantly excited by punishment rather than reward and represent the anticipation of punishment or the motivation for avoidance. Furthermore, inhibiting these neurons impairs punishment-based learning without affecting reward learning or movement. These results establish a major role of striosomal neurons in behaviors reinforced by punishment and moreover uncover functions of the direct pathway unaccounted for in classic models.

17.
Emerg Microbes Infect ; 9(1): 2105-2113, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32893735

RESUMO

The global pandemic of coronavirus disease 2019 (COVID-19) is a disaster for human society. A convenient and reliable neutralization assay is very important for the development of vaccines and novel drugs. In this study, a G protein-deficient vesicular stomatitis virus (VSVdG) bearing a truncated spike protein (S with C-terminal 18 amino acid truncation) was compared to that bearing the full-length spike protein of SARS-CoV-2 and showed much higher efficiency. A neutralization assay was established based on VSV-SARS-CoV-2-Sdel18 pseudovirus and hACE2-overexpressing BHK21 cells (BHK21-hACE2 cells). The experimental results can be obtained by automatically counting the number of EGFP-positive cells at 12 h after infection, making the assay convenient and high-throughput. The serum neutralizing titer measured by the VSV-SARS-CoV-2-Sdel18 pseudovirus assay has a good correlation with that measured by the wild type SARS-CoV-2 assay. Seven neutralizing monoclonal antibodies targeting the receptor binding domain (RBD) of the SARS-CoV-2 S protein were obtained. This efficient and reliable pseudovirus assay model could facilitate the development of new drugs and vaccines.


Assuntos
Anticorpos Neutralizantes/sangue , Anticorpos Antivirais/sangue , Betacoronavirus/imunologia , Infecções por Coronavirus/diagnóstico , Testes de Neutralização/métodos , Pneumonia Viral/diagnóstico , Glicoproteína da Espícula de Coronavírus/imunologia , Animais , Anticorpos Monoclonais/imunologia , Anticorpos Neutralizantes/imunologia , Anticorpos Antivirais/imunologia , Linhagem Celular , Chlorocebus aethiops , Cricetinae , Pandemias , Glicoproteína da Espícula de Coronavírus/genética , Células Vero , Vírus da Estomatite Vesicular Indiana/genética , Vírus da Estomatite Vesicular Indiana/imunologia
18.
Medicine (Baltimore) ; 99(33): e21674, 2020 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-32872034

RESUMO

The aim of the study was to observe the efficacy of nonsurgical treatment with Chinese herbal medicine (CHM) for chronic subdural hematoma (CSDH). This study includes clinical results of a STROBE-compliant retrospective study.Forty patients diagnosed with CSDH were recruited from outpatient. Different CHM prescriptions were dispensed for each patient based on syndrome differentiation until the patient had a stable neurologic condition for 2 weeks and/or CSDH completely resolved according to the computed tomography scan. Markwalder grading scale for neurologic symptoms and head computed tomography scan for hematoma volumes were performed before and after CHM treatment to evaluate efficacy.Patients received uninterrupted CHM treatment for 2.81 ±â€Š1.45 months (0.75-6 months). The hematoma volume significantly reduced from 73.49 ±â€Š35.43 mL to 14.72 ±â€Š15.94 mL (P < .001). The Markwalder grading scale scores of patients at the end of CHM treatment decreased significantly, from 1.3 ±â€Š0.69 to 0.15 ±â€Š0.36 (P < .001). Ninety percent of the patients showed >50% decrease in the hematoma volume and complete improvement in neurologic symptoms. The linear regression analysis suggested that change in hematoma was significantly related to the duration of CHM treatment (R = 0.334; P < .001; Y = 25.03 + 11.91X). Leonurus heterophyllus Sweet (Yi-Mu-Cao, 90.5%), Semen persicae (Tao-Ren, 88.8%), and Acorus tatarinowii Schott (Shi-Chang-Pu, 86.2%) were the top 3 single Chinese herbs prescribed in CHM treatment.The CHM treatment for CSDH based on syndrome differentiation with appropriate duration relieved neurologic symptoms quickly and promoted hematoma absorption effectively. It could be an effective nonsurgical therapy for CSDH.


Assuntos
Medicamentos de Ervas Chinesas/administração & dosagem , Hematoma Subdural Crônico/tratamento farmacológico , Adulto , Idoso , Idoso de 80 Anos ou mais , China , Esquema de Medicação , Feminino , Hematoma Subdural Crônico/diagnóstico por imagem , Hematoma Subdural Crônico/patologia , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Tomografia Computadorizada por Raios X
19.
J Am Heart Assoc ; 9(18): e016128, 2020 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-32914661

RESUMO

Background Carotid plaques with expansive arterial remodeling are closely related to cerebral ischemic events. Although S100A4 (S100 calcium-binding protein A4) is expressed in atherosclerotic lesions, its role in atherosclerotic plaque progression remains unknown. In this study, we examined the association between carotid arterial expansive remodeling and S100A4 expression. Methods and Results Preoperative high-resolution magnetic resonance imaging was used to assess luminal stenosis and vascular remodeling in patients undergoing carotid endarterectomy. To examine murine carotid atherosclerosis, we induced experimental lesions by flow cessation in apolipoprotein E-deficient mice fed a high-fat diet. The role of S100A4 in plaque formation and smooth muscle cell proliferation was investigated in vivo and in vitro, respectively. Human carotid arterial expansive remodeling showed positive correlations with the expression of S100A4, MMP2, and MMP9. S100A4 mRNA levels were positively correlated with those of MMP2, MMP9, and MMP13. S100A4 was expressed in vascular smooth muscle cells (VSMCs) and VSMC-derived foam cells in the plaque shoulder and marginal areas. S100A4 expression increased concomitantly with plaque formation in our animal model. Exogenous recombinant S100A4 protein enhanced the levels of Mmp2, Mmp9, and Mmp13 and the cell proliferation ability in VSMCs. A chemotaxis assay indicated that extracellular S100A4 functions as a chemoattractant for VSMCs. Conclusions S100A4 expression was elevated in human carotid plaques and showed a positive correlation with the degree of expansive remodeling. S100A4-positive VSMC-derived cells are considered to play an important role in carotid expansive remodeling.

20.
Ann Ital Chir ; 91: 327-333, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32877386

RESUMO

OBJECTIVE: This study aims to evaluate the execution of robot-assisted laparoscopic nephroureterectomy without repositioning the patient. METHODS: The clinical data of 9 patients who underwent robot-assisted laparoscopic nephroureterectomy between May 2017 and November 2018 were analyzed, proceeding in a single position, without repositioning the patient. This involved 5 men and 4 women, with an average age of 61.67 ± 10.37 years and an average body mass index (BMI) of 24.78 ± 3.84. We considered the duration of the intervention, the blood loss, the duration of the hospital stay, the duration of maintenance of the drainage and the follow-up on all patients, with or without complications and recurrence of the tumor. RESULTS: The intervention was completed in all 9 cases. The average duration of the intervention was 242.89 ± 13.37 minutes, the average blood loss was 166.67 ± 70.71 ml, the average hospitalization time was 2 ± 0.71 days, the average time drainage maintenance was 5.11 ± 1.05 days and the average follow-up times without complications and tumor recurrence were 12.56 ± 6.19 months. CONCLUSION: Robot-assisted laparoscopic nephroureterectomy without repositioning the patient during the procedure simplifies the procedure and shortens the duration of the procedure. It is also a safe, effective and feasible minimally invasive treatment method. KEY WORDS: Nephroureterectomy, Robot-assisted laparoscopic, Tumor recurrence, Single position, Upper tract urothelial carcinoma.

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