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3.
J Cosmet Dermatol ; 2021 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-34706154

RESUMO

BACKGROUND: How diseases associated with skin discoloration affect the quality of life (QoL) has rarely been examined. In this study, we directly compared the QoL and willingness to pay (WTP) among patients with skin disorders associated with facial discoloration. METHODS: A single-center, cross-sectional study consisting of patients seeking treatment for facial discoloration was performed. All patients completed the dermatology life quality index (DLQI) and the short form 36 (SF-36) health survey questionnaires. Additionally, information regarding monthly income and the WTP for a complete cure of the affected condition was obtained. RESULTS: Patients with melasma (58), lentigo (56), postinflammatory hyperpigmentation (5), vitiligo (51), acne-induced erythema (10), and rosacea (31) were enrolled. Vitiligo, acne-induced erythema, and rosacea patients reported significantly worse QoL compared to lentigines or melasma patients as evaluated by DLQI. Using SF-36, rosacea patients were affected mentally the most as they reported the worst mental component scores, and the scores were significantly worse than that of melasma patients (rosacea vs. melisma = 46.5 vs. 52.7, p = 0.007). Vitiligo patients had the worst general health scores. Although there were no differences in the WTP among different diseases, the vitiligo group had the highest percentage of patients willing to pay more than 40% of their monthly income for a complete cure. CONCLUSIONS: The impact on QoL is different among skin diseases associated with facial discoloration. Only vitiligo patients reported worse general health as compared to the general population, explaining the discordance between life quality scores and WTP in these patients.

4.
Front Neurol ; 12: 743110, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34675874

RESUMO

Objective: We conducted a survey to assess vaccination coverage, vaccination willingness, and variables associated with vaccination hesitancy to provide evidence on coronavirus disease (COVID-19) vaccination strategies. Methods: This anonymous questionnaire study conducted a multicenter, cross-sectional survey of outpatients and inpatients with epilepsy (PWE) registered in epilepsy clinics, in 2021, in 10 hospitals in seven cities of Shandong Province. Results: A total of 600 questionnaires were distributed, and 557 valid questionnaires were returned. A total of 130 people were vaccinated against COVID-19. Among 427 unvaccinated participants, 69.32% (296/427) were willing to receive the COVID-19 vaccine in the future, and the remaining 30.68% (131/427) were unwilling to receive vaccination. Most (89.9%) of the participants believed that the role of vaccination was crucial in response to the spread of COVID-19. A significant association was found between willingness to receive the COVID-19 vaccine and the following variables: age, marital status, level of education, occupation, residence, seizure type, and seizure control after antiepileptic drug therapy. It is noteworthy that education level, living in urban areas, and seizure freedom were significantly related to willingness to receive COVID-19 vaccination. Conclusions: Vaccination is a key measure for the prevention and control of COVID-19, and most PWE are willing to be vaccinated. Vaccine safety, effectiveness, and accessibility are essential in combatting vaccine hesitation and increasing vaccination rates.

5.
Medicine (Baltimore) ; 100(37): e27234, 2021 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-34664866

RESUMO

ABSTRACT: Various studies have revealed an association between cigarette smoking and increased risk for multiple sclerosis (MS). However, its role in neuromyelitis optica spectrum disorder (NMOSD) remains elusive. Therefore, in the present case-control study, we aimed to assess the association of active and passive cigarette smoking with the risk of MS and NMOSD.Thirty-six patients with NMOSD, 46 patients with MS, and 122 healthy individuals were included in this study. Standardized questionnaires and telephone interviews were used to collect information regarding the active and passive cigarette smoking behaviors of the patients and normal controls.The risk of MS was significantly higher among smokers than among nonsmokers (odds ratio = 2.166, 95% confidence interval: 1.109-4.170; P = .027). Further analysis of the risk between active and passive smokers, male smokers and nonsmokers showed no statistical difference. However, neither smokers nor active smokers had a greater or lower risk of NMOSD than their nonsmoking counterparts.Our preliminary study showed no significant association between cigarette smoking and the risk of NMOSD, strongly suggesting that, unlike MS, cigarette smoking might not confer NMOSD susceptibility, at least in the Northern Han Chinese population.


Assuntos
Fumar Cigarros/efeitos adversos , Neuromielite Óptica/diagnóstico , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Índice de Massa Corporal , Criança , China/epidemiologia , Fumar Cigarros/epidemiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Neuromielite Óptica/epidemiologia , Razão de Chances , Fatores de Risco , Inquéritos e Questionários , Tabaco/efeitos adversos , Tabaco/fisiologia
6.
Chin J Physiol ; 64(4): 186-193, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34472449

RESUMO

Adult hippocampal neurogenesis (AHN) is suppressed by chronic stress. The negative effect of stress is mainly attributed to increased levels of stress hormones (e.g. glucocorticoids, GCs). Exercise enhances AHN, yet it also stimulates GC secretion. To delineate the paradoxical role of GCs, we took the advantage of a unique mouse strain (L/L) which exhibits an inert response to stress-induced secretion of GCs to study the role of GCs in exercise-induced AHN. Our results showed that basal corticosterone (CORT), the main GCs in rodents, levels were similar between the L/L mice and wild-type (WT) mice. However, levels of CORT in the L/L mice were barely altered and significantly lower than those of the WT mice during treadmill running (TR). AHN was enhanced by 4 weeks of TR in the WT mice, but not L/L mice. WT mice that received daily injection of CORT to evoke serum CORT levels similar to those during exercise for 4 weeks did not affect AHN, whereas injection with large amount of CORT inhibited AHN. Taken together, our results indicated that exercise-related elevation of CORT participates in exercise-enhanced AHN. CORT alone is not sufficient to elicit AHN and may inhibit AHN if the levels are high.


Assuntos
Corticosterona , Corrida , Animais , Glucocorticoides , Hipocampo , Camundongos , Neurogênese
7.
Org Lett ; 23(20): 7865-7872, 2021 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-34582193

RESUMO

A palladium-catalyzed enantioselective Heck cyclization/dearomatization cascade via capturing the cyclized Heck π-allylpalladium intermediate by ß-naphthols is reported, which provides a new strategy for the construction of chiral indole-terpenoid frameworks. This method affords indole-functionalized ß-naphthalenone compounds bearing an all-carbon-substituted quaternary chiral center in excellent yields (up to 92%) and enantioselectivities (up to 94% ee). In addition, the utility of this method is showcased by the gram-scale syntheses and diverse transformations of the dearomatized products.

9.
Int J Ophthalmol ; 14(9): 1302-1309, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34540603

RESUMO

Glycolysis produces large amounts of adenosine triphosphate (ATP) in a short time. The retinal vascular endothelium feeds itself primarily through aerobic glycolysis with less ATP. But when it generates new vessels, aerobic glycolysis provides rapid and abundant ATP support for angiogenesis, and thus inhibition of glycolysis in endothelial cells can be a target for the treatment of neovascularization. Aerobic glycolysis has a protective effect on Müller cells, and it can provide with a target for visual protection and maintenance of the blood-retinal barrier. Under physiological conditions, the mitochondria of RPE can use lactic acid produced by photoreceptor cells as an energy source to provide ATP for survival. In pathological conditions, because RPE cells avoid their oxidative damage by increasing glycolysis, a large number of glycolysis products accumulate, which in turn has a toxic effect on photoreceptor cells. This shows that stabilizing the function of RPE mitochondria may become a target for the treatment of diseases such as retinal degeneration. The decrease of aerobic glycolysis leads to the decline of photoreceptor cell function and impaired vision; therefore, aerobic glycolysis of stable photoreceptor cells provides a reliable target for delaying vision loss. It is of great significance to study the role of glycolysis in various retinal cells for the targeted treatment of ocular fundus diseases.

10.
Cancer Gene Ther ; 2021 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-34145425

RESUMO

Primary cytoreductive surgery with platinum-taxane-based chemotherapy is the standard treatment for ovarian cancer (OC) patients; however, resistance to chemotherapy is a contributing factor to OC mortality. Paclitaxel (PTX), the most widely used taxane, has become the first-line drug against OC. The molecular mechanism of PTX resistance is different from that of platinum-based agents and is still not completely elucidated. Our previous study showed that glucose-regulated protein 78 (GRP78) is involved in the resistance of OC cells to PTX. However, little is known regarding endogenous inhibitors of this gene. MicroRNAs (miRNAs) play critical roles in the regulation of gene expression; therefore, we sought to identify miRNA(s) with potential to target GRP78 under the hypothesis that miRNA(s) could serve as potential therapeutic targets. Here, we show that miR-181c, predicted to target GRP78, was downregulated in PTX-resistant OC cells and tissues. MiR-181c downregulated GRP78 expression and induced apoptosis by directly targeting its 3'-untranslated region (UTR). Overexpression of miR-181c sensitized resistant OC to PTX by inhibiting the PI3K/Akt pathway in vitro and in vivo. Taken together, our findings indicate that the delivery of miR-181c can efficiently suppress GRP78 expression and GRP78-mediated PTX resistance in OC and suggest that this strategy has therapeutic potential.

11.
J Exp Bot ; 72(13): 4888-4903, 2021 06 22.
Artigo em Inglês | MEDLINE | ID: mdl-33940615

RESUMO

GIBBERELLIN MYB GENE (GAMYB), UNDEVELOPED TAPETUM1 (UDT1), TDR INTERACTING PROTEIN2 (TIP2/bHLH142), TAPETUM DEGENERATION RETARDATION (TDR), and ETERNAL TAPETUM 1/DELAYED TAPETUM DEGENERATION (EAT1/DTD) are important transcription factors that play a crucial role during pollen development in rice. This study demonstrates that bHLH142 acts downstream of UDT1 and GAMYB and works as a 'hub' in these two pollen pathways. We show that GAMYB modulates bHLH142 expression through specific binding to the MYB motif of the bHLH142 promoter during the early stage of pollen development, while TDR acts as a transcriptional repressor of the GAMYB modulation of bHLH142 by binding to the E-box close to the MYB motif on the promoter. Altered expression of these transcription factors highlights that a tight, precise, and coordinated regulation among them is essential for normal pollen development. Most notably, we show that the regulatory pathways of GAMYB and UDT1 rely on bHLH142 in a direct and indirect manner, respectively, and function in different tissues with distinct biological roles during pollen development. This study advances our understanding of the molecular mechanisms of rice pollen development.


Assuntos
Oryza , Flores/genética , Flores/metabolismo , Regulação da Expressão Gênica de Plantas , Oryza/genética , Oryza/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Pólen/genética , Pólen/metabolismo , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo
12.
Front Oncol ; 11: 672052, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33996600

RESUMO

Posttransplant lymphoproliferative disorder (PTLD) is a rare complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT) with poor prognosis. We report a patient with PTLD involved central nervous system (CNS) who treated with zanubrutinib, a second-generation Bruton tyrosine kinase (BTK) inhibitor. Our report supports the efficacy of bruton tyrosine kinase inhibitor zanubrutinib in the treatment of CNS-PTLD, which provides a new therapeutic option.

13.
Environ Technol ; : 1-14, 2021 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-33886443

RESUMO

Antibiotic pollution of water resources is a global problem, and the development of new treatments for destroying antibiotics in water is a priority research. We successfully manufactured recyclable magnetic Fe3O4/g-C3N4 through the electrostatic self-assembly method. Selecting tetracycline (TC) as the target pollutant, using Fe3O4/g-C3N4 and H2O2 developed a heterogeneous optical Fenton system to remove TC under visible light. Fe3O4/g-C3N4 was systematically characterized by SEM, TEM, XRD, FTIR, XPS, DRS, and electrochemical methods. The removal efficiency of 7% Fe3O4/g-C3N4 at pH = 3, H2O2 = 5 mM, and catalyst dosage of 1.0 g/L can reach 99.8%. After magnetic separation, the Fe3O4/g-C3N4 photocatalyst can be recycled five times with minimal efficiency loss. The excellent degradation performance of the prepared catalyst may be attributed to the proper coupling interface between Fe3O4 and g-C3N4 which promotes the separation and transfer of photogenerated electrons. Photogenerated electrons can also accelerate the conversion of Fe3+ to Fe2+, thereby producing more ˙OH. The new Fe3O4/g-C3N4 can be used as a raw material for advanced oxidation of water contaminated by refractory antibiotics.

15.
Genomics ; 113(1 Pt 2): 429-439, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32889043

RESUMO

Decapoda is one of the most diverse crustacean orders, and has become an important research subject. However, the phylogenetic relationships among the main lineages of Decapoda remain uncertain, especially in the order Brachyura. Herein, we sequenced the whole mitochondrial genome of V. litterata and constructed a phylogenetic tree to understand its phylogenetic relationships with other species. The results showed that the mitochondrial genome of V. litterata was generally similar to mitogenomes of Metazoa reported in the literature, with a size of 16,247 bp, 37 genes, and a control region. Both AT-skew and GC-skew were negative, indicating more abundant Cs and Ts than Gs and As. The gene arrangement of V. litterata is identical to those of Eriocheir hepuensis, Cyclograpsus granulosus, Hemigrapsus sanguineus, Helicana wuana, and Helice tientsinensis but differs from the pancrustacean ground pattern and typical arrangement of Brachyuran crabs. Phylogenetic reconstruction showed that V. litterata belongs to the Varunidae.

16.
Environ Sci Pollut Res Int ; 28(4): 4731-4738, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32951166

RESUMO

Soil contamination caused by long-term application of metsulfuron-methyl and tribenuron-methyl has become an issue of increasing concern. In our previous study, strain Chenggangzhangella methanolivorans CHL1, capable of efficiently degrading sulfonylurea herbicides, was isolated. Here, the bioremediation potential of strain CHL1 was assessed for soil polluted with metsulfuron-methyl or tribenuron-methyl in a pot experiment. The growth parameters of waxy maize were measured on day 21 of the pot experiment. Additionally, the residues of metsulfuron-methyl and tribenuron-methyl in soils were analyzed, and the soil microbial community was investigated using a phospholipid fatty acids (PLFAs) method on days 1, 7, 14, and 21. The results indicated that strain CHL1 greatly accelerated the degradation of metsulfuron-methyl and tribenuron-methyl in soils. The degradation rates in the treatments inoculated with strain CHL1were all more than 91% after 7 days, significantly higher than the 25-36% degradation measured in non-inoculated treatments. Furthermore, strain CHL1 reduced the negative effects of tribenuron-methyl and metsulfuron-methyl on waxy maize growth, especially the primary root length. Moreover, inoculation with strain CHL1 also reduced the effects of tribenuron-methyl and metsulfuron-methyl on soil microbial biomass, diversity, and community structure. The present study demonstrates that strain CHL1 has great potential application to remediate soil contaminated with metsulfuron-methyl or tribenuron-methyl.


Assuntos
Herbicidas , Poluentes do Solo , Sulfonatos de Arila , Biodegradação Ambiental , Methylocystaceae , Solo , Microbiologia do Solo , Poluentes do Solo/análise
17.
Seizure ; 84: 122-128, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33348235

RESUMO

The astroglial network connected through gap junctions assembling from connexins physiologically balances the concentrations of ions and neurotransmitters around neurons. Astrocytic dysfunction has been associated with many neurological disorders including epilepsy. Dissociated gap junctions result in the increased activity of connexin hemichannels which triggers brain pathophysiological changes. Previous studies in patients and animal models of epilepsy indicate that the reduced gap junction coupling from assembled connexin hemichannels in the astrocytes may play an important role in epileptogenesis. This abnormal cell-to-cell communication is now emerging as an important feature of brain pathologies and being considered as a novel therapeutic target for controlling epileptogenesis. In particular, candidate drugs with ability of inhibition of connexin hemichannel activity and enhancement of gap junction formation in astrocytes should be explored to prevent epileptogenesis and control epilepsy.


Assuntos
Astrócitos , Conexinas , Animais , Comunicação Celular , Junções Comunicantes , Humanos , Neurônios
18.
Huan Jing Ke Xue ; 42(1): 1-8, 2021 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-33372451

RESUMO

Since 2013, the Chinese government implemented the Air Pollution Prevention and Control Action Plan. As a result, the atmospheric concentrations of sulfate reduced significantly, whereas the nitrate concentrations remain relatively high due to the excess of ammonia (NH3). To date, there is no official observation network monitoring NH3 concentrations in China. Previous studies have focused on NH3 or ammonium (NH4+) separately. These limitations hinder a complete understanding of their dynamic changes due to the rapid gas-to-particle conversion. In this study, the concentrations of NH3 and NH4+ were measured concurrently in urban Beijing during autumn 2019 utilizing an acid-coated denuder-filter combination with a time resolution from 2 h (PM2.5>35 µg·m-3) to 5 h (PM2.5<35 µg·m-3). The mean concentrations of NH3 and NH4+ during the study were (4.1±2.9)µg·m-3 and (1.7±1.4) µg·m-3, respectively. The temporal patterns of NH3 or NH4+ coincided with that of PM2.5, CO, and NO2 throughout the sampling period. The diurnal distributions of NH3 were bimodal, both on polluted (PM2.5>75 µg·m-3) and non-polluted (PM2.5<75 µg·m-3) days, peaking at 21:30-05:30 and 05:30-08:30, respectively. The NH3 concentrations on polluted days were relatively lower during 17:30-21:30, which may be related to higher wind speeds. In contrast to NH3, NH4+ had an obvious peak during 17:30-21:30 due to the formation of ammonium nitrate. The meteorological conditions favor the gas-to-particle conversion on polluted days, resulting in a lower NH3/NH4+ ratio of 0.8. However, this value may reach 2.8 on non-polluted days. The concentrations of NH3, CO, NO2, SO2, and PM2.5 in the emission control period showed a significant increase greater than or comparable to those in the non-control period by 54.2%, 40.4%, 33.3%, 0%, and 49.4%, respectively. This result shows that the stagnant conditions offset the benefit of emission control actions implemented during and before the National Celebration Day.

19.
Onco Targets Ther ; 13: 10829-10840, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33149601

RESUMO

Objective: Lung cancer is the first leading cause of cancer-related deaths both worldwide and in China and threatens human health and quality of life. New drugs and therapeutic methods are urgently needed. Our study evaluated the roles of dihydroartemisinin (DHA) in lung cancer and further explored its underlying mechanisms. Methods: CCK-8, colony formation and trypan blue exclusion assays were used to detect the cell viability, colony formation ability and cell death. qRT-PCR and Western blotting assays were applied to analyze the expressions of key molecules. Results: DHA inhibited the proliferation and colony formation abilities and enhanced the cell death and induced ferroptosis of lung NCI-H23 and XWLC-05 cancer cells. DHA reduced PRIM2 expression and silencing PRIM2 mimicked the inhibitory roles on proliferation and colony formation and promotive roles on cell death and ferroptosis of DHA in lung NCI-H23 and XWLC-05 cancer cells. We further found that DHA treatment and loss of PRIM2 reduced the GSH level and increased the cellular lipid ROS and mitochondrial MDA levels, and further downregulated the expressions of SLC7A11 and ß-catenin in lung cancer cells, respectively. Exogenetic overexpression of PRIM2 recovered the inhibitory effects of DHA on proliferation and colony formation in lung NCI-H23 cancer cells, meanwhile loss of PRIM2 sensitizes NCI-H23 cells to DHA therapy. In vivo experiment further showed that DHA treatment significantly suppressed the tumor growth and downregulated PRIM2 and SLC7A11. Conclusion: Our study suggested that DHA inhibited the proliferation, colony formation and enhanced cell death and induced ferroptosis of lung cancer cells by inactivating PRIM2/SLC7A11 axis. Loss of PRIM2 induced ferroptosis might developed to be a novel therapeutic method in lung cancer therapy.

20.
Front Genet ; 11: 536640, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33240311

RESUMO

Mitochondrial genomes (mitogenomes) are important for understanding molecular evolution and phylogenetic relationships. The complete mitogenome of Perisesarma bidens was determined, which is 15,641 bp in length. The A + T content of P. bidens mitogenome was 74.81%. The AT skew was slightly negative (-0.021). The 22 tRNAs ranged from 65 to 73 bp and were highly A + T biased. All tRNA genes had typical cloverleaf structures, except for the trnS1 gene, which lacked a dihydrouridine (DHU) arm. The gene order within the P. bidens mitogenome was identical to the pancrustacean ground pattern, except for the translocation of the trnH. Additionally, the gene order of trnI-trnQ-trnM in pancrustacean ground pattern became trnQ-trnI-trnM in P. bidens. Phylogenetic analyses supported the inclusion of P. bidens in Sesarmidae and the promotion of Sesarminae to Sesarmidae. The results will help us to better understand the status and evolutionary history of Grapsoidea crabs.

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