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1.
Medicine (Baltimore) ; 98(35): e16999, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31464953

RESUMO

RATIONALE: Adenoid cystic carcinoma (ACC) rarely occurs in the digestive tract, particularly in the gastroesophageal junction. PATIENT CONCERNS: A 44-year-old male vomiting blood was admitted to our hospital. Endoscopic ultrasound showed a 2.2 × 3.0 cm submucosal tumor in the gastroesophageal junction. DIAGNOSIS: According to the histopathological examination, the tumor was composed predominantly of ductal epithelial and myoepithelial cells. Immunohistochemical staining revealed that the tumor expressed cytokeratin, cluster of differentiation 117, p63, and calponin. Based on these findings, ACC was diagnosed. INTERVENTIONS: Endoscopic submucosal dissection (ESD) was performed to remove the tumor. As the margins of the ESD specimen were positive, the patient underwent total gastrectomy with D2 lymphadenectomy. Finally, neither residual tumor nor lymphatic metastasis was detected in the surgical specimens. OUTCOMES: No sign of recurrence has been detected during 36 months of follow-up as of October 2018. LESSONS: ESD may be an alternative treatment for cardial ACC invading the submucosa.


Assuntos
Carcinoma Adenoide Cístico/patologia , Junção Esofagogástrica/patologia , Neoplasias Gástricas/patologia , Adulto , Carcinoma Adenoide Cístico/cirurgia , Ressecção Endoscópica de Mucosa/métodos , Gastrectomia/métodos , Humanos , Masculino , Neoplasias Gástricas/cirurgia
2.
Medicine (Baltimore) ; 98(6): e14477, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30732216

RESUMO

RATIONALE: The efficiency and tolerance of long-term adjuvant imatinib treatment for patient who underwent complete resection of a localized recurrent gastrointestinal stromal tumor (GIST) was unknown. PATIENT CONCERNS: A 45-year-old man underwent complete resection of an intestinal GIST in August 2001. Four years later, a giant (11 × 8 × 6 cm) recurrent GIST located in the retroperitoneum was detected. DIAGNOSIS: The recurrent tumor was positive for CD117 by immunohistochemistry. INTERVENTIONS: The recurrent tumor was completely resected after 4 months of effective imatinib treatment (400 mg/day), and the patient continued imatinib treatment postoperatively. In June 2011, imatinib treatment was stopped for 3 weeks because of hepatitis B infection, and resumed with a reduced dose level of 300 mg/day when liver function recovered. In March 2017, imatinib treatment was interrupted again for 12 days because the patient underwent cholecystectomy. OUTCOMES: In December 2017, a computed tomography scan showed no signs of tumor recurrence. To date, the patient has been under adjuvant imatinib treatment for >12 years without severe side effects. The plasma concentration of imatinib (detected in February 2018) was trough concentration (Cmin) 1015.7 ng/mL and peak concentration (Cmax) 1550.5 ng/mL. LESSONS: This case report highlights the active role of long-term (>12 years) imatinib treatment after complete resection of localized recurrent GIST.


Assuntos
Antineoplásicos/uso terapêutico , Tumores do Estroma Gastrointestinal/tratamento farmacológico , Tumores do Estroma Gastrointestinal/patologia , Mesilato de Imatinib/uso terapêutico , Antineoplásicos/administração & dosagem , Quimioterapia Adjuvante , Tumores do Estroma Gastrointestinal/cirurgia , Humanos , Mesilato de Imatinib/administração & dosagem , Masculino , Pessoa de Meia-Idade , Recidiva Local de Neoplasia
3.
Exp Ther Med ; 12(5): 3195-3200, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27882137

RESUMO

The present study reported the case of a Chinese boy who was diagnosed with Moyamoya disease (MMD) associated with Graves' disease (GD). An overactivation of von Willebrand factor (vWF) and coagulation factor VIII (FVIII) was identified in the plasma of the patient. Thiamazole and metoprolol treatment was thus administrated. After 2 months of treatment, the patient's thyroid function returned to normal and the neurological symptoms improved gradually. At the same time, the activities of vWF and FVIII were depressed. During the 20-month follow-up, information regarding the neurological symptoms, cerebrovascular imaging, thyroid function, thyroid autoantibodies and coagulation parameters was collected. High levels of thyroid autoantibodies persisted throughout the follow-up period, while other coagulation parameters remained in the normal range. In conclusion, considering the vital role of vWF and FVIII in vascular diseases, it is hypothesized that these two factors may serve an important role in the occurrence of GD associated with MMD.

5.
Neuroreport ; 27(11): 858-63, 2016 08 03.
Artigo em Inglês | MEDLINE | ID: mdl-27306591

RESUMO

Human enteric neural stem cells (hENSCs) proliferate and differentiate into neurons and glial cells in response to a complex network of neurotrophic factors to form the enteric nervous system. The primary aim of this study was to determine the effect of basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) on in-vitro expansion and differentiation of postnatal hENSCs-containing enteric neurosphere cells. Enteric neurosphere cells were isolated from rectal polyp specimens of 75 children (age, 1-13 years) and conditioned with bFGF, EGF, bFGF+EGF, or plain culture media. Proliferation of enteric neurosphere cells was examined using the methyl thiazolyl tetrazolium colorimetric assay over 7 days of culture. Fetal bovine serum (10%) was added to induce the differentiation of parental enteric neurosphere cells, and differentiated offspring cells were immunophenotyped against p75 neutrophin receptor (neural stem cells), peripherin (neuronal cells), and glial fibrillary acidic protein (glial cells). Combining bFGF and EGF significantly improved the proliferation of enteric neurosphere cells compared with bFGF or EGF alone (both P<0.01) throughout 7 days of culture. The addition of bFGF drove a significantly greater proportion of enteric neurosphere cells to differentiate into neuronal cells than that of EGF (P<0.01), whereas addition of EGF resulted in significantly more glial differentiation compared with addition of bFGF (P<0.01). Combining bFGF and EGF drove enteric neurosphere cells to differentiate into neuronal cells in a proportion similar to glial cells. Our results showed that the combination of bFGF and EGF significantly enhanced the proliferation and differentiation of postnatal hENSCs-containing enteric neurosphere cells in vitro.


Assuntos
Diferenciação Celular/efeitos dos fármacos , Sistema Nervoso Entérico/citologia , Fator de Crescimento Epidérmico/farmacologia , Proteína Glial Fibrilar Ácida/farmacologia , Células-Tronco Neurais/efeitos dos fármacos , Neuroglia/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Adolescente , Proliferação de Células/efeitos dos fármacos , Criança , Pré-Escolar , Colorimetria , Feminino , Humanos , Lactente , Masculino , Proteínas do Tecido Nervoso/metabolismo , Neuroglia/metabolismo , Neurônios/metabolismo , Receptores de Fator de Crescimento Neural/metabolismo
6.
Sheng Li Ke Xue Jin Zhan ; 47(3): 194-202, 2016 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-29888883

RESUMO

In the past decades, diabetes, in particular type 2 diabetes (T2D)mainly characterized by global insulin resistance and pancreatic beta cell failure, had become epidemic and a severe public health threat worldwide with the development of economy and change of lifestyle.The interactions between environment factors and genetic background play vital roles in the development and progression of T2D.More recently, it had been revealed that non-coding RNA including microRNA (miRNA)and long noncoding RNA (LncRNA)are widely involved inthe regulation of glucose and lipid metabolism. So far, it had been established that deregulated miRNA and LncRNA profile in main metabolic tissues is tightly associated with T2D,and intensive studies on non-coding RNAs had shed light on understanding the pathogen-esis of T2D.The current review aimed to briefly summarize and discuss the latest findings regarding the role and mechanism of miRNAs and LncRNAs in the regulation hepatic glucose and lipid metabolism.


Assuntos
Metabolismo dos Lipídeos , Diabetes Mellitus Tipo 2 , Glucose , Humanos , Resistência à Insulina , Células Secretoras de Insulina , Fígado , RNA não Traduzido
7.
J Biomed Inform ; 58 Suppl: S164-70, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26279500

RESUMO

In the United States, about 600,000 people die of heart disease every year. The annual cost of care services, medications, and lost productivity reportedly exceeds 108.9 billion dollars. Effective disease risk assessment is critical to prevention, care, and treatment planning. Recent advancements in text analytics have opened up new possibilities of using the rich information in electronic medical records (EMRs) to identify relevant risk factors. The 2014 i2b2/UTHealth Challenge brought together researchers and practitioners of clinical natural language processing (NLP) to tackle the identification of heart disease risk factors reported in EMRs. We participated in this track and developed an NLP system by leveraging existing tools and resources, both public and proprietary. Our system was a hybrid of several machine-learning and rule-based components. The system achieved an overall F1 score of 0.9185, with a recall of 0.9409 and a precision of 0.8972.


Assuntos
Doenças Cardiovasculares/epidemiologia , Mineração de Dados/métodos , Complicações do Diabetes/epidemiologia , Registros Eletrônicos de Saúde/organização & administração , Narração , Processamento de Linguagem Natural , Idoso , California/epidemiologia , Doenças Cardiovasculares/diagnóstico , Estudos de Coortes , Comorbidade , Segurança Computacional , Confidencialidade , Complicações do Diabetes/diagnóstico , Feminino , Humanos , Incidência , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Reconhecimento Automatizado de Padrão/métodos , Medição de Risco/métodos , Vocabulário Controlado
8.
World J Gastroenterol ; 21(8): 2573-6, 2015 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-25741171

RESUMO

A 67-year-old female presented with a primary hepatic gastrointestinal stromal tumor that was detected by computed tomography and diagnosed based on histopathological and genetic analyses. The tumor was microscopically composed of spindle cells and epithelioid cells, and immunohistochemistry results showed positive staining for CD117 and CD34 expression. A genetic analysis revealed a heterozygous point mutation and deletion in exon 11 of c-KIT. After an R0 resection, imatinib mesylate was administered for 1 year until its use was discontinued due to severe side effects. Two years after the original operation, the tumor recurred in the residual liver and was completely resected again. Imatinib mesylate was administered for 2 years until it was replaced by sunitinib malate because of disease progression. The patient has survived for 53 mo after undergoing a sequential therapy consisting of surgical excision, imatinib and sunitinib.


Assuntos
Antineoplásicos/administração & dosagem , Benzamidas/administração & dosagem , Tumores do Estroma Gastrointestinal/terapia , Hepatectomia , Indóis/administração & dosagem , Neoplasias Hepáticas/terapia , Piperazinas/administração & dosagem , Inibidores de Proteínas Quinases/administração & dosagem , Pirimidinas/administração & dosagem , Pirróis/administração & dosagem , Idoso , Antineoplásicos/efeitos adversos , Benzamidas/efeitos adversos , Biomarcadores Tumorais/análise , Biomarcadores Tumorais/genética , Biópsia , Quimioterapia Adjuvante , Análise Mutacional de DNA , Substituição de Medicamentos , Feminino , Tumores do Estroma Gastrointestinal/química , Tumores do Estroma Gastrointestinal/genética , Tumores do Estroma Gastrointestinal/patologia , Humanos , Mesilato de Imatinib , Imuno-Histoquímica , Neoplasias Hepáticas/química , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/patologia , Mutação , Piperazinas/efeitos adversos , Inibidores de Proteínas Quinases/efeitos adversos , Pirimidinas/efeitos adversos , Sunitinibe , Fatores de Tempo , Tomografia Computadorizada por Raios X , Resultado do Tratamento
9.
J Dermatol ; 42(1): 90-3, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25387725

RESUMO

We report the clinical and pathological findings of two cases of Bowen's disease (BD) with features resembling myrmecia wart, and tried to find evidence of human papillomavirus (HPV) infection in such lesions by immunohistological staining, genotyping systems, polymerase chain reaction (PCR) and electron microscopy. Both cases manifested unique barnacle-like hyperkeratotic nodules or plaques clinically, and microscopically proliferation of atypical keratinocytes involving the entire thickness of the epidermis, hypergranulosis with eosinophilic and/or basophilic inclusion bodies, features that mimicked myrmecia wart. Electron microscopy revealed myrmecia inclusion-like large intranuclear and cytoplasmic electron-dense bodies. Immunohistological staining with anti-HPV antibody, genotyping systems for HPV infection and specific PCR designed to detect HPV-1 L1 sequences failed to detect evidence of HPV infection. P16(INK4a) was overexpressed in the atypical keratinocytes of both cases. This finding suggests that the pathogenesis of these two BD may involve certain unknown or undetectable HPV, or reflect disturbances of the Rb signaling pathway unrelated to HPV infection. The unique "myrmecioid" clinicopathological features in our cases suggest that this type of lesion may be a new variant of BD.


Assuntos
Doença de Bowen/diagnóstico , Pele/patologia , Idoso de 80 Anos ou mais , Doença de Bowen/ultraestrutura , Doença de Bowen/virologia , Diagnóstico Diferencial , Feminino , Humanos , Masculino , Verrugas/diagnóstico
11.
World J Gastroenterol ; 16(14): 1808-10, 2010 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-20380018

RESUMO

Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor in the alimentary tract. To the best of our knowledge, few cases have been reported in the literature about the peripheral lymph node metastasis of GIST. Here we report an unusual case of gastric GIST with inguinal lymph nodes metastasis. After the metastatic lymph nodes were resected, the. patient started to take imatinib 400 mg/d for 12 mo. There were no signs of tumor recurrence at follow-up after 29 mo. This case suggests that the inguinal lymph nodes can be a potential metastatic site of GIST.


Assuntos
Tumores do Estroma Gastrointestinal/patologia , Neoplasias Gástricas/patologia , Antineoplásicos/uso terapêutico , Benzamidas , Tumores do Estroma Gastrointestinal/secundário , Tumores do Estroma Gastrointestinal/terapia , Humanos , Mesilato de Imatinib , Canal Inguinal , Metástase Linfática , Masculino , Pessoa de Meia-Idade , Piperazinas/uso terapêutico , Pirimidinas/uso terapêutico , Neoplasias Gástricas/terapia
12.
J Surg Oncol ; 100(7): 606-10, 2009 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-19697360

RESUMO

OBJECTIVE: Gastrointestinal stromal tumors (GISTs) occur rarely in the duodenum. The characteristics of duodenal GIST have not been well clarified. The aim of this study is to clarify the characteristics and surgical prognosis of patients with primary duodenal GIST. METHODS: Data of patients with surgically treated primary duodenal GIST were retrospectively analyzed. Immunohistochemical expressions of p53, p16, and Ki-67 were evaluated to explain the prognosis. RESULTS: Compared with gastric or small intestinal GISTs in historical studies, duodenal GISTs had a relatively smaller size, lower mitotic count, lower Ki-67 LI, lower p16 loss, and similar p53 expression. The 1- and 3-year recurrence-free survival rates of patients with complete resection were 100 and 95.2%. CONCLUSION: Patients with completely resected primary duodenal GIST seem to have a more favorable prognosis. This may be related to the different expressions of some immunohistological makers compared with GISTs of other locations.


Assuntos
Neoplasias Duodenais/metabolismo , Neoplasias Duodenais/mortalidade , Tumores do Estroma Gastrointestinal/metabolismo , Tumores do Estroma Gastrointestinal/mortalidade , Adulto , Idoso , Idoso de 80 Anos ou mais , Inibidor p16 de Quinase Dependente de Ciclina , Neoplasias Duodenais/patologia , Neoplasias Duodenais/cirurgia , Feminino , Seguimentos , Tumores do Estroma Gastrointestinal/patologia , Tumores do Estroma Gastrointestinal/cirurgia , Humanos , Imuno-Histoquímica , Antígeno Ki-67/metabolismo , Masculino , Pessoa de Meia-Idade , Proteínas de Neoplasias/metabolismo , Recidiva Local de Neoplasia , Prognóstico , Estudos Retrospectivos , Taxa de Sobrevida , Proteína Supressora de Tumor p53/metabolismo
13.
Scand J Gastroenterol ; 42(9): 1138-40, 2007 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-17710683

RESUMO

Gastrointestinal stromal tumours (GISTs) are the most common mesenchymal tumours of the GI tract. Although a great deal of experience has been obtained with imatinib mesylate in patients with unresectable or metastatic GISTs, its role in the neoadjuvant setting is not well established. In this paper, we describe a case of successful resection of a giant recurrent GIST with imatinib mesylate as neoadjuvant therapy.


Assuntos
Antineoplásicos/uso terapêutico , Neoplasias Gastrointestinais/cirurgia , Recidiva Local de Neoplasia/cirurgia , Piperazinas/uso terapêutico , Pirimidinas/uso terapêutico , Adulto , Benzamidas , Quimioterapia Adjuvante , Neoplasias Gastrointestinais/tratamento farmacológico , Neoplasias Gastrointestinais/patologia , Humanos , Mesilato de Imatinib , Masculino , Recidiva Local de Neoplasia/tratamento farmacológico , Recidiva Local de Neoplasia/patologia
14.
Plant Signal Behav ; 1(2): 59-66, 2006 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19521477

RESUMO

Suberin, a cell specific, wall-associated biopolymer, is formed during normal plant growth and development as well as in response to stress conditions such as wounding. It is characterized by the deposition of both a poly(phenolic) domain (SPPD) in the cell wall and a poly(aliphatic) domain (SPAD) thought to be deposited between the cell wall and plasma membrane. Although the monomeric components that comprise the SPPD and SPAD are well known, the biosynthesis and deposition of suberin is poorly understood. Using wound healing potato tubers as a model system, we have tracked the flux of carbon into the aliphatic monomers of the SPAD in a time course fashion. From these analyses, we demonstrate that newly formed fatty acids undergo one of two main metabolic fates during wound-induced suberization: (1) desaturation followed by oxidation to form the 18:1 omega-hydroxy and dioic acids characteristic of potato suberin, and (2) elongation to very long chain fatty acids (C20 to C28), associated with reduction to 1-alkanols, decarboxylation to n-alkanes and minor amounts of hydroxylation. The partitioning of carbon between these two metabolic fates illustrates metabolic regulation during wound healing, and provides insight into the organization of fatty acid metabolism.

15.
Zhongguo Zhong Yao Za Zhi ; 28(12): 1188-90, 2003 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-15617509

RESUMO

OBJECTIVE: To study the effects of Dihydromyricetin (DMY) on antilipid-peroxidation. METHOD: The antilipid-peroxidation of DMY on heart, liver, brain tissue homogenate and mitochondria was measured by the determination of malondiadehyde (MDA) induced by Fe2+ -Vit C, Fe2+ -H2O2, Fe-Cys with TBA spectrometric method. RESULT: DMY could inhibit the lipid peroxidation of homogenate and mitochondria. The inhibition exhibited concentration-dependent manner. CONCLUSION: DMY has good antilipid-peroxidation effect, which is worth studing further.


Assuntos
Antioxidantes/farmacologia , Flavonóis/farmacologia , Peroxidação de Lipídeos/efeitos dos fármacos , Ampelopsis/química , Animais , Antioxidantes/isolamento & purificação , Encéfalo/metabolismo , Relação Dose-Resposta a Droga , Flavonóis/isolamento & purificação , Fígado/metabolismo , Masculino , Malondialdeído/metabolismo , Mitocôndrias/metabolismo , Dilatação Mitocondrial/efeitos dos fármacos , Miocárdio/metabolismo , Plantas Medicinais/química , Ratos
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