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1.
J Hazard Mater ; 416: 125822, 2021 08 15.
Artigo em Inglês | MEDLINE | ID: mdl-34492784

RESUMO

Biochar adsorbents for removing As(III) suffer from the problems of low adsorption capacity and ineffective removal. Herein, a granular MgO-embedded biochar (g-MgO-Bc) adsorbent is fabricated in the form of millimeter-sized particles through a simple gelation-calcination method using chitosan as biochar sources. High-density MgO nanoparticles are evenly dispersed throughout the biochar matrix and can be fully exposed to As(III) through the rich pores in g-MgO-Bc. These features endow the adsorbent with a high adsorption capacity of 249.1 mg/g for As(III). The g-MgO-Bc can efficiently remove As(III) over a wide pH of 3-10. The coexisting carbonate, nitrate, sulfate, silicate, and humic acid exert a negligible influence on As(III) removal. 300 µg/L of As(III) can be purified to far below 10 µg/L using only 0.3 g/L g-MgO-Bc. The spent g-MgO-Bc could be well regenerated by simple calcination. In fixed-bed column experiments, the effective treatment volume of As(III)-spiked groundwater achieves 1500 BV (30 L) (3 g of adsorbent, solution flow rate of 2.0 mL/min, C0 = 50 µg/L). The Mg(OH)2 generated in situ in g-MgO-Bc is responsible for the adsorption of As(III) through the inner-sphere complex mechanism. The work would extend the potential applicability of biochar adsorbent for As(III) removal to a great extent.


Assuntos
Óxido de Magnésio , Poluentes Químicos da Água , Adsorção , Carvão Vegetal , Cinética , Porosidade , Água , Poluentes Químicos da Água/análise
2.
ACS Appl Mater Interfaces ; 13(36): 42843-42851, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34482694

RESUMO

The oxygen evolution reaction has become the bottleneck of electrochemical water splitting for its sluggish kinetics. Developing high-efficiency and low-cost non-noble-metal oxide electrocatalysts is crucial but challenging for industrial application. Herein, superhydrophilic/superaerophobic hierarchical nanoneedle/microflower arrays of Ce-substituted Co3O4 (CexCo3-xO4) in situ grown on the nickel foam are successfully constructed. The hierarchical architecture and superhydrophilic/superaerophobic interface can be facilely regulated by controlling the introduction of Ce into Co3O4. The unique feature of hierarchical architecture and superhydrophilic/superaerophobic interface is in favor of electrolyte penetration and bubbles release. In addition, the presence of oxygen vacancy and Ce endows the catalyst with enhanced intrinsic activity. Benefiting from these advantages, the optimized Ce0.12Co2.88O4 catalyst shows a superior electrocatalytic performance for the oxygen evolution reaction (OER) with an overpotential of 282 mV at 20 mA cm-2, and a Tafel slope of 81.4 mV dec-1. The turnover frequency of 0.0279 s-1 for Ce0.12Co2.88O4 is 9.3 times larger than that for Co3O4 at an overpotential of 350 mV. Moreover, the optimized Ce0.12Co2.88O4 catalyst shows a robust long-term stability in alkaline media.

3.
Laser Photon Rev ; 15(8)2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34539926

RESUMO

Imaging systems with miniaturized device footprint, real-time processing speed and high resolution three-dimensional (3D) visualization are critical to broad biomedical applications such as endoscopy. Most of existing imaging systems rely on bulky lenses and mechanically refocusing to perform 3D imaging. Here, we demonstrate GEOMScope, a lensless single-shot 3D microscope that forms image through a single layer of thin microlens array and reconstructs objects through an innovative algorithm combining geometrical-optics-based pixel back projection and background suppressions. We verify the effectiveness of GEOMScope on resolution target, fluorescent particles and volumetric objects. Comparing to other widefield lensless imaging devices, we significantly reduce the required computational resource and increase the reconstruction speed by orders of magnitude. This enables us to image and recover large volume 3D object in high resolution with near real-time processing speed. Such a low computational complexity is attributed to the joint design of imaging optics and reconstruction algorithms, and a joint application of geometrical optics and machine learning in the 3D reconstruction. More broadly, the excellent performance of GEOMScope in imaging resolution, volume, and reconstruction speed implicates that geometrical optics could greatly benefit and play an important role in computational imaging.

4.
Photonics Res ; 9(3): B57-B70, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-34532505

RESUMO

The need for high-speed imaging in applications such as biomedicine, surveillance and consumer electronics has called for new developments of imaging systems. While the industrial effort continuously pushes the advance of silicon focal plane array image sensors, imaging through a single-pixel detector has gained significant interests thanks to the development of computational algorithms. Here, we present a new imaging modality, Deep Compressed Imaging via Optimized-Pattern Scanning (DeCIOPS), which can significantly increase the acquisition speed for a single-detector-based imaging system. We project and scan an illumination pattern across the object and collect the sampling signal with a single-pixel detector. We develop an innovative end-to-end optimized auto-encoder, using a deep neural network and compressed sensing algorithm, to optimize the illumination pattern, which allows us to reconstruct faithfully the image from a small number of samples, and with a high frame rate. Compared with the conventional switching-mask based single-pixel camera and point scanning imaging systems, our method achieves a much higher imaging speed, while retaining a similar imaging quality. We experimentally validated this imaging modality in the settings of both continuous-wave (CW) illumination and pulsed light illumination and showed high-quality image reconstructions with a high compressed sampling rate. This new compressed sensing modality could be widely applied in different imaging systems, enabling new applications which require high imaging speed.

5.
Clin Neurol Neurosurg ; 208: 106836, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34371385

RESUMO

BACKGROUND: Coagulopathy is a severe complication of traumatic brain injury (TBI) and can cause secondary injuries and death. Decrease of FVII activity contributes to the coagulopathy and progressive hemorrhagic injury (PHI) in patients with isolated TBI. Some polymorphic loci of coagulation factor VII (FVII) are shown to be essential for FVII activity. However, the relationship between FVII gene polymorphisms and coagulopathy in patients with isolated TBI is still unknown. Therefore, the present study aimed to investigate the relationship between FVII gene polymorphisms and plasma FVIIa levels, and assess whether FVII polymorphisms were associated with TBI-related coagulopathy, PHI, and 6 months GOS in patients with isolated TBI. METHODS: One-hundred-forty-nine patients with isolated TBI (from East of China) admitted to Huashan Hospital's Neurological Trauma Center from March 2012 to March 2016 were enrolled in this study. The Polymorphism-Polymerase Chain Reaction (PCR) method was used to analyze the five FVII polymorphism loci (-323P0/P10, R353Q, -401G/T, -402G/A, and -670A/C) of these patients. Patients' blood was collected to test the activated partial thromboplastin time, international normalized ratio, platelet, and FVIIa concentrations. Other clinical characteristics were also recorded. RESULTS: The minor alleles of three genotypes of -323 P0/P10, R353Q, and -401G/T each independently associated with 23.3%, 28.6%, and 27.6% lower FVIIa levels, respectively. These polymorphisms explained 21% of the total variance of FVIIa levels (adjusted R2:0.206). The genotype of -323P0/P10 was an independent risk factor for coagulopathy (OR = 2.77, p = 0.043) and PHI (OR = 3.47, p = 0.03) after adjustment for confounding factors in the logistic regression model. Polymorphisms of FVII were not independently associated with 6 months Glasgow Outcome Scale (GOS) of isolated TBI patients. CONCLUSION: -323P0/P10, R353Q, and -401 G/T genotypes were associated with FVIIa levels. -323P0/P10 genotype was independently associated with traumatic coagulopathy and PHI in isolated TBI patients.

7.
Proc Natl Acad Sci U S A ; 118(14)2021 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-33811144

RESUMO

Neural processing of sensory information is strongly influenced by context. For instance, cortical responses are reduced to predictable stimuli, while responses are increased to novel stimuli that deviate from contextual regularities. Such bidirectional modulation based on preceding sensory context is likely a critical component or manifestation of attention, learning, and behavior, yet how it arises in cortical circuits remains unclear. Using volumetric two-photon calcium imaging and local field potentials in primary visual cortex (V1) from awake mice presented with visual "oddball" paradigms, we identify both reductions and augmentations of stimulus-evoked responses depending, on whether the stimulus was redundant or deviant, respectively. Interestingly, deviance-augmented responses were limited to a specific subset of neurons mostly in supragranular layers. These deviance-detecting cells were spatially intermixed with other visually responsive neurons and were functionally correlated, forming a neuronal ensemble. Optogenetic suppression of prefrontal inputs to V1 reduced the contextual selectivity of deviance-detecting ensembles, demonstrating a causal role for top-down inputs. The presence of specialized context-selective ensembles in primary sensory cortex, modulated by higher cortical areas, provides a circuit substrate for the brain's construction and selection of prediction errors, computations which are key for survival and deficient in many psychiatric disorders.

8.
Adv Exp Med Biol ; 1293: 613-639, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33398846

RESUMO

A critical neuroscience challenge is the need to optically image and manipulate neural activity with high spatiotemporal resolution over large brain volumes. The last three decades have seen the development of calcium imaging to record activity from neuronal populations, as well as optochemistry and optogenetics to optically manipulate neural activity. These methods are typically implemented with wide-field or laser-scanning microscopes. While the former approach has a good temporal resolution, it generally lacks spatial resolution or specificity, particularly in scattering tissues such as the nervous system; meanwhile, the latter approach, particularly when combined with two-photon excitation, has high spatial resolution and specificity but poor temporal resolution. As a new technique, holographic microscopy combines the advantages of both approaches. By projecting a holographic pattern on the brain through a spatial light modulator, the activity of specific groups of neurons in 3D brain volumes can be imaged or stimulated with high spatiotemporal resolution. In a combination of other techniques such as fast scanning or temporal focusing, this high spatiotemporal resolution can be further improved. Holographic microscopy enables all-optical interrogating of neural activity in 3D, a critical tool to dissect the function of neural circuits.


Assuntos
Holografia , Optogenética , Encéfalo/diagnóstico por imagem , Neurônios , Fótons
10.
Alzheimers Res Ther ; 12(1): 89, 2020 07 23.
Artigo em Inglês | MEDLINE | ID: mdl-32703308

RESUMO

BACKGROUND: Neurogenesis is significantly impaired in the brains of both human patients and experimental animal models of Alzheimer's disease (AD). Although deep brain stimulation promotes neurogenesis, it is an invasive technique that may damage neural circuitry along the path of the electrode. To circumvent this problem, we assessed whether intracranial electrical stimulation to the brain affects neurogenesis in a mouse model of Alzheimer's disease (5xFAD). METHODS AND RESULTS: We used Ki67, Nestin, and doublecortin (DCX) as markers and determined that neurogenesis in both the subventricular zone (SVZ) and hippocampus were significantly reduced in the brains of 4-month-old 5xFAD mice. Guided by a finite element method (FEM) computer simulation to approximately estimate current and electric field in the mouse brain, electrodes were positioned on the skull that were likely to deliver stimulation to the SVZ and hippocampus. After a 4-week program of 40-Hz intracranial alternating current stimulation (iACS), neurogenesis indicated by expression of Ki67, Nestin, and DCX in both the SVZ and hippocampus were significantly increased compared to 5xFAD mice who received sham stimulation. The magnitude of neurogenesis was close to the wild-type (WT) age-matched unmanipulated controls. CONCLUSION: Our results suggest that iACS is a promising, less invasive technique capable of effectively stimulating the SVZ and hippocampus regions in the mouse brain. Importantly, iACS can significantly boost neurogenesis in the brain and offers a potential treatment for AD.


Assuntos
Doença de Alzheimer , Doença de Alzheimer/terapia , Animais , Simulação por Computador , Modelos Animais de Doenças , Hipocampo , Humanos , Camundongos , Neurogênese
11.
Surg Infect (Larchmt) ; 21(8): 677-683, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31944897

RESUMO

Background: Intra-cranial bacterial infection is a serious complication in emergency neurosurgical patients, and few data are available on the trends of these infections and the rates of antibacterial drug resistance. Methods: We surveyed retrospectively the patients with intra-cranial bacterial infection in our emergency neurosurgical center between January 2009 and December 2018. Demographic characteristics, causative bacteria, and antimicrobial susceptibility test results were collected. Results: A total of 100 patients with intra-cranial bacterial infection, 1.41% of our patients, were enrolled. There were 123 strains of bacteria cultured from the cerebrospinal fluid (CSF), consisting of 96 strains of gram-negative (GN) bacteria and 27 strains of gram-positive (GP) bacteria. Compared with the GP bacteria, the percentage of GN bacteria increased to 75.0% of the total near the end of the study. Compared with Acinetobacter baumannii, Klebsiella pneumoniae also showed an uptrend. Most of the bacteria were multi-drug-resistant (MDR). We further analyzed the consistency of CSF and sputum cultures from the same patient. The ratio of Klebsiella pneumoniae and Acinetobacter baumannii to the corresponding strains in the CSF were 77.0% and 62.0%. Conclusions: The bacteria recovered from the CSF were mainly GN. The tendency of GN bacteria showed an uptrend, especially for Klebsiella pneumoniae. Furthermore, most of the bacteria were MDR.

12.
Cell ; 178(2): 447-457.e5, 2019 07 11.
Artigo em Inglês | MEDLINE | ID: mdl-31257030

RESUMO

Neurons in cortical circuits are often coactivated as ensembles, yet it is unclear whether ensembles play a functional role in behavior. Some ensemble neurons have pattern completion properties, triggering the entire ensemble when activated. Using two-photon holographic optogenetics in mouse primary visual cortex, we tested whether recalling ensembles by activating pattern completion neurons alters behavioral performance in a visual task. Disruption of behaviorally relevant ensembles by activation of non-selective neurons decreased performance, whereas activation of only two pattern completion neurons from behaviorally relevant ensembles improved performance, by reliably recalling the whole ensemble. Also, inappropriate behavioral choices were evoked by the mistaken activation of behaviorally relevant ensembles. Finally, in absence of visual stimuli, optogenetic activation of two pattern completion neurons could trigger behaviorally relevant ensembles and correct behavioral responses. Our results demonstrate a causal role of neuronal ensembles in a visually guided behavior and suggest that ensembles implement internal representations of perceptual states.


Assuntos
Comportamento Animal , Córtex Visual/fisiologia , Animais , Área Sob a Curva , Cálcio/metabolismo , Holografia , Processamento de Imagem Assistida por Computador , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Neurônios/metabolismo , Optogenética/métodos , Estimulação Luminosa , Fótons , Curva ROC
13.
Cell Rep ; 27(7): 2229-2240.e4, 2019 05 14.
Artigo em Inglês | MEDLINE | ID: mdl-31091458

RESUMO

To capture the emergent properties of neural circuits, high-speed volumetric imaging of neural activity at cellular resolution is needed. Here, we introduce wavelength multiplexing to perform fast volumetric two-photon imaging of cortical columns (>2,000 neurons in 10 planes at 10 vol/s), using two different calcium indicators, an electrically tunable lens and a spatial light modulator. We image the activity of neuronal populations from layers 2/3 to 5 of primary visual cortex from awake mice, finding a lack of columnar structures in orientation responses and revealing correlations between layers which differ from trial to trial. We also simultaneously image functional correlations between presynaptic layer 1 axons and postsynaptic layer 2/3 neurons. Wavelength multiplexing enhances high-speed volumetric microscopy and can be combined with other optical multiplexing methods to easily boost imaging throughput.


Assuntos
Microscopia de Fluorescência por Excitação Multifotônica/métodos , Condução Nervosa/fisiologia , Neurônios/fisiologia , Transmissão Sináptica/fisiologia , Córtex Visual/fisiologia , Animais , Axônios/fisiologia , Cálcio/metabolismo , Feminino , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Neurônios/citologia , Sinapses/fisiologia , Córtex Visual/citologia
14.
Nat Biotechnol ; 37(4): 378-380, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30872818
15.
Chin Med J (Engl) ; 131(16): 1990-1998, 2018 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-30082532

RESUMO

Objective: Due to the special anatomical structure and pathophysiological mechanism of the central nervous system (CNS), there is a big difference between the repair of brain injury and other systems of the body. More and more evidence shows that targetedly reducing the autoimmune response of brain tissue without affecting the immune function in other parts of the body will be the best optimized treatment for brain injury. Data Sources: This review was based on data in articles published in PubMed up to June 5, 2017, with the following keywords: "immune tolerance", "traumatic brain injury", and "central nervous system". Study Selection: Original articles and critical reviews on immune tolerance and brain damage were selected for this review. References of the retrieved articles were also screened to search for potentially relevant papers. Results: The CNS is isolated from the immune system through the blood-brain barrier. After brain injury, brain antigens are released into the systemic circulation to induce damaging immune responses. Immune tolerance can effectively reduce the brain edema and neurological inflammatory response after brain injury, which is beneficial to the recovery of neurological function. The clinical application prospect and theoretical research value of the treatment of immune tolerance on traumatic brain injury (TBI) is worth attention. Conclusions: The establishment of immune tolerance mechanism has a high clinical value in the treatment of TBI. It opens up new opportunities for the treatment of brain damage.


Assuntos
Lesões Encefálicas Traumáticas/terapia , Tolerância Imunológica , Imunoterapia , Encéfalo/imunologia , Lesões Encefálicas Traumáticas/imunologia , Sistema Nervoso Central , Humanos
16.
Curr Opin Neurobiol ; 50: 211-221, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29660600

RESUMO

Optical imaging methods are powerful tools in neuroscience as they can systematically monitor the activity of neuronal populations with high spatiotemporal resolution using calcium or voltage indicators. Moreover, caged compounds and optogenetic actuators enable to optically manipulate neural activity. Among optical methods, computer-generated holography offers an enormous flexibility to sculpt the excitation light in three-dimensions (3D), particularly when combined with two-photon light sources. By projecting holographic light patterns on the sample, the activity of multiple neurons across a 3D brain volume can be simultaneously imaged or optically manipulated with single-cell precision. This flexibility makes two-photon holographic microscopy an ideal all-optical platform to simultaneously read and write activity in neuronal populations in vivo in 3D, a critical ability to dissect the function of neural circuits.


Assuntos
Holografia/métodos , Neurônios/fisiologia , Imagem Óptica/métodos , Optogenética/métodos , Animais , Encéfalo/citologia , Encéfalo/diagnóstico por imagem
17.
Elife ; 72018 02 07.
Artigo em Inglês | MEDLINE | ID: mdl-29412138

RESUMO

The simultaneous imaging and manipulating of neural activity could enable the functional dissection of neural circuits. Here we have combined two-photon optogenetics with simultaneous volumetric two-photon calcium imaging to measure and manipulate neural activity in mouse neocortex in vivo in three-dimensions (3D) with cellular resolution. Using a hybrid holographic approach, we simultaneously photostimulate more than 80 neurons over 150 µm in depth in layer 2/3 of the mouse visual cortex, while simultaneously imaging the activity of the surrounding neurons. We validate the usefulness of the method by photoactivating in 3D selected groups of interneurons, suppressing the response of nearby pyramidal neurons to visual stimuli in awake animals. Our all-optical approach could be used as a general platform to read and write neuronal activity.


Assuntos
Imageamento Tridimensional , Microscopia de Fluorescência por Excitação Multifotônica , Vias Neurais/anatomia & histologia , Vias Neurais/fisiologia , Córtex Visual/anatomia & histologia , Córtex Visual/fisiologia , Animais , Camundongos , Optogenética
18.
Clin Exp Hypertens ; 40(3): 213-217, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29420090

RESUMO

OBJECTIVES: (1) To investigate the expression patterns of MΦ1 and MΦ2 phenotype markers of peripheral blood monocyte (PBMC)-derived macrophages in atherosclerosis patients and healthy controls, as well as the expression correlation among these genes. (2) To elucidate whether a high level of liver X receptor α (LXRα) expression is associated with anti-inflammatory MΦ2-type polarization. DESIGN: Peripheral blood monocytes (PBMCs) were obtained from 28 patients with carotid artery plaques and 10 normal persons, who did not have carotid artery plaques. M1 and M2 phenotype markers were analyzed after cellular differentiation into macrophages. Human macrophages derived from healthy donors were transfected with plasmid DNA encoding LXRα and control null-plasmids. Gene expression levels were quantified after further differentiation. RESULTS: Three genes (LXRα, CD68, and CD36) were expressed at a significantly lower rate in the atherosclerotic group than normal patients. There were correlations between the expression of LXRα, CD68, and peroxisome proliferator-activated receptor (PPARγ), and between CD163, CD36 and scavenger receptor class A (SRA1). Macrophages over-expressing LXRα exhibited enhanced expression level of MΦ2-type genes and decreased expression level of MΦ1-type genes. CONCLUSIONS: PBMCs from healthy persons were predisposed to the MΦ2 differentiation phenotype, which exhibits elevated cholesterol uptake and anti-inflammatory properties. LXRα over-expression polarizes macrophages towards the anti-inflammatory MΦ2 phenotype.


Assuntos
Aterosclerose/genética , Diferenciação Celular/genética , Expressão Gênica , Receptores X do Fígado/genética , Macrófagos/metabolismo , Antígenos CD/genética , Antígenos de Diferenciação Mielomonocítica/genética , Antígenos CD36/genética , Estudos de Casos e Controles , Células Cultivadas , Humanos , Hipertensão/metabolismo , Inflamação/fisiopatologia , Leucócitos Mononucleares , Macrófagos/fisiologia , PPAR gama/genética , Fenótipo , Cultura Primária de Células , Receptores de Superfície Celular/genética , Receptores Depuradores Classe A/genética , Transfecção
19.
Opt Express ; 25(15): 18462-18473, 2017 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-28789331

RESUMO

Integrated optical circuits are poised to open up an array of novel applications. A vibrant field of research has emerged around the monolithic integration of optical components onto the silicon substrates. Typically, single mode optical fibers deliver the external light to the chip, and submicron single-mode waveguides then guide the light on-chip for further processing. For such technology to be viable, it is critically important to be able to efficiently couple light into and out of the chip platform, and between the different components, with low losses. Due to the large volume mismatch between a fiber and silicon waveguide (on the order of 600), it has been extremely challenging to obtain high coupling efficient with large tolerance. To date, demonstrated coupling has been relatively lossy and effective coupling requires impractical alignment of optical components. Here, we propose the use of a high contrast metastructure (HCM) that overcomes these issues, and effectively couples the off-chip, out-of-plane light waves into on-chip, in-plane waveguides. By harnessing the resonance properties of the metastructure, we show that it is possible to spatially confine the incoming free-space light into subwavelength dimensions with a near-unity (up to 98%) efficiency. The underlying coupling mechanism is analyzed and designs for practical on-chip coupler and reflector systems are presented. Furthermore, we explore the two-dimensional HCM as an ultra-compact wavelength multiplexer with superior efficiency (90%).

20.
PLoS Comput Biol ; 13(8): e1005685, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28771570

RESUMO

Progress in modern neuroscience critically depends on our ability to observe the activity of large neuronal populations with cellular spatial and high temporal resolution. However, two bottlenecks constrain efforts towards fast imaging of large populations. First, the resulting large video data is challenging to analyze. Second, there is an explicit tradeoff between imaging speed, signal-to-noise, and field of view: with current recording technology we cannot image very large neuronal populations with simultaneously high spatial and temporal resolution. Here we describe multi-scale approaches for alleviating both of these bottlenecks. First, we show that spatial and temporal decimation techniques based on simple local averaging provide order-of-magnitude speedups in spatiotemporally demixing calcium video data into estimates of single-cell neural activity. Second, once the shapes of individual neurons have been identified at fine scale (e.g., after an initial phase of conventional imaging with standard temporal and spatial resolution), we find that the spatial/temporal resolution tradeoff shifts dramatically: after demixing we can accurately recover denoised fluorescence traces and deconvolved neural activity of each individual neuron from coarse scale data that has been spatially decimated by an order of magnitude. This offers a cheap method for compressing this large video data, and also implies that it is possible to either speed up imaging significantly, or to "zoom out" by a corresponding factor to image order-of-magnitude larger neuronal populations with minimal loss in accuracy or temporal resolution.


Assuntos
Encéfalo/diagnóstico por imagem , Biologia Computacional/métodos , Processamento de Imagem Assistida por Computador/métodos , Neurônios/citologia , Algoritmos , Animais , Camundongos , Neurofisiologia , Peixe-Zebra
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