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1.
Dalton Trans ; 2021 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-34792061

RESUMO

Three novel iron dinitrogen hydrides, [FeH(iPr-PSiMeP)(N2)(PMe3)] (1), [FeH(iPr-PSiPhP)(N2)(PMe3)] (2), and [FeH(iPr-PSiPh)(N2)(PMe3)] (3), supported by a silyl ligand are synthesized for the first time by changing the electronic effect and steric hindrance of the ligands through the reaction of ligands L1-L3 with Fe(PMe3)4 in a nitrogen atmosphere. The ligands containing an electron-donating group with large steric hindrance on the phosphorus atom are beneficial for the formation of dinitrogen complexes. A penta-coordinate iron hydride [FeH(iPr-PSiPh)(PMe3)2] (4) was formed through the reaction of ligand L3 with Fe(PMe3)4 in an argon atmosphere under the same conditions. The reactions between complexes 1-3 with an atmospheric pressure of dihydrogen gas resulted in Fe(II) dihydrides, [(iPr-PSiMe(µ-H)P)Fe(H)2(PMe3)] (5), [(iPr-PSiPh(µ-H)P)Fe(H)2(PMe3)] (6) and [(iPr-PSiPh(µ-H))Fe(H)2(PMe3)2] (7), with an η2-(Si-H) coordination. The isolation of dihydrides 5-7 demonstrates the ability of the dinitrogen complexes 1-3 to realize the activation of dihydrogen under ambient temperature and pressure. The molecular structures of complexes 1-7 were elucidated by single crystal X-ray diffraction analysis. The iron dinitrogen hydrides 1-3 are effective catalysts for the silylation of dinitrogen under ambient conditions and among them 3 is the best catalyst.

2.
Sci Total Environ ; : 151694, 2021 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-34798085

RESUMO

Waste activated sludge (WAS), as the byproducts of wastewater treatment plants, has been greatly produced. With high cost and environmental risk of WAS disposal, to explore a low-cost and environment-friendly technology has been a great challenge. Considering that WAS is a collection of organic matters, anaerobic fermentation has been selected as a sustainable way to simultaneously recover resources and reduce environmental pollution. To recover short-chain fatty acids (SCFAs) has gained great concern because of the high value-added application and high-efficiency production process. Considering the temperature in some areas of the world can reach to below 0 °C, this study proposed an efficient strategy, i.e., stepwise freezing and thawing treatment, to promote SCFAs production. The maximal production of SCFAs, i.e., 246 mg COD/g volatile suspended solid, was obtained with the shortened retention time of five days. Mechanistic studies showed that the solubilization of both extracellular polymeric substances (EPSs) and microbial cells could be accelerated, with the EPSs removal of 58.3% for proteins and 59.0% for polysaccharides. Also, the hydrolysis process was promoted to provide more substrates for subsequent acidogenisis, and the functional microorganisms, such as Romboutsia, Paraclostridium, Macellibacteroides and Conexibacter, were greatly enriched, with a total abundance of 26.2%. Moreover, compared to control, methanogenesis was inhibited at a shortened sludge retention time (e.g., five days), which benefited to the accumulation of SCFAs, but the methane production was increased by 25.2% at a longer sludge retention time (e.g., ten days). Thus, these findings of this work may provide some new solutions for the enhanced resource recovery from WAS, and further for carbon-neutral operation of wastewater treatment plants.

3.
ACS Biomater Sci Eng ; 2021 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-34817980

RESUMO

Zinc shows promise for bone repair applications, while its strength and ductility require to be improved. Carbon nanotubes (CNTs) are exceptional reinforcements due to their superior strength, ultrahigh Young's modulus, and large aspect ratio. However, their strong agglomeration and weak interfacial bonding with the matrix are key bottleneck problems restricting the reinforcing effect. In this study, Ag nanoparticles were in situ reduced on CNTs and then the CNT@Ag powders were used to prepare Zn-CNT@Ag implants by laser powder bed fusion. Results showed that Ag reacted with Zn to form a "knot"-like AgZn3 phase. It had the same lattice structure (HCP) with Zn, which indicated a good lattice matching with the matrix, thus improving the dispersion of CNTs. More significantly, the knot played a "rivet" role and enhanced the load transfer capacity, which advantaged the CNT strengthening effects by assisting in transferring the load. Moreover, it enhanced the heterogeneous nucleation effects during solidification, which weakened the texture strength of the matrix and thus increased the ductility by improving the sliding capacity. The compressive yield strength, ultimate tensile strength, and elongation of the Zn-CNT@Ag implant were increased by 22, 26, and 17% in comparison to Zn-CNTs. Moreover, the Zn-CNT@Ag implant exhibited favorable antibacterial activity with a bacterial inhibition rate of 87.79%. Additionally, it also exhibited a suitable degradation rate and acceptable biocompatibility.

4.
Bioresour Technol ; : 126337, 2021 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-34780904

RESUMO

This study proposed a novel and high-efficiency strategy, i.e., freezing followed by low-temperature thermal treatment, to significantly promote short-chain fatty acids (SCFAs) production from waste activated sludge compared to traditional freezing/thawing treatment. The maximal production of SCFAs was 212 mg COD/g VSS with a shortened retention time of five days, and the potentially recovered carbon source, including SCFAs, soluble polysaccharides and proteins, reached 321 mg COD/g VSS, increased by 92.1 and 28.3% compared to sole freezing and thermal treatment. Both the solubilization and hydrolysis steps of WAS were accelerated, and the acid-producing microorganisms, such as Macellibacteroides, Romboutsia and Paraclostridium, were greatly enriched, with a total abundance of 13.9%, which was only 0.54% in control. Interestingly, the methane production was inhibited at a shortened retention time, resulting in SCFAs accumulation, whereas it was increased by 32.0% at a longer sludge retention time, providing a potential solution for energy recovery from WAS.

5.
Front Cardiovasc Med ; 8: 738044, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34722671

RESUMO

Background: Coronavirus disease 2019 (COVID-19) has outbroken in China and subsequently spread worldwide since the end of 2019. Chest computed tomography (CT) plays an important role in the diagnosis of lung diseases, but its value in the diagnosis of cardiac injury remains unknown. Methods: We enrolled 241 consecutive hospitalized patients (aged 61 ± 16 years, 115 males) with laboratory-confirmed COVID-19 at Renmin Hospital of Wuhan University from January 11 to March 2, 2020. They were divided into two groups according to whether major adverse cardiovascular events (MACEs) occurred during the follow-up. The anteroposterior diameter of the left atrium (LAD), the length of the left ventricle (LV), and cardiothoracic ratio (CTR) were measured. The values of myocardial CT were also recorded. Results: Of 241 patients, 115 patients (47.7%) had adverse cardiovascular events. Compared with no MACEs, patients with MACEs were more likely to have bilateral lesions (95.7% vs. 86.5%, p = 0.01). In multivariable analysis, bronchial wall thickening would increase the odds of MACEs by 13.42 (p = 0.01). LAD + LV and CTR was the best predictor for MACEs (area under the curve = 0.88, p < 0.001) with a sensitivity of 82.6% and a specificity of 80.2%. Plasma high-sensitivity troponin I levels in patients with cardiac injury showed a moderate negative correlation with minimum CT value (R 2 = -0.636, p < 0.001). Conclusions: Non-contrast chest CT can be a useful modality for detection cardiac injury and provide additional value to predict MACEs in COVID-19 patients.

6.
BMC Genomics ; 22(Suppl 3): 739, 2021 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-34649506

RESUMO

BACKGROUND: Long non-coding RNAs (lncRNAs) play vital roles in many important biological processes in plants. Currently, a large fraction of plant lncRNA studies center at lncRNA identification and functional analysis. Only a few plant lncRNA studies focus on understanding their evolutionary history, which is crucial for an in-depth understanding of lncRNAs. Therefore, the integration of large volumes of plant lncRNA data is required to deeply investigate the evolution of lncRNAs. RESULTS: We present a large-scale evolutionary analysis of lncRNAs in 25 flowering plants. In total, we identified 199,796 high-confidence lncRNAs through data integration analysis, and grouped them into 5497 lncRNA orthologous families. Then, we divided the lncRNAs into groups based on the degree of sequence conservation, and quantified the various characteristics of 756 conserved Arabidopsis thaliana lncRNAs. We found that compared with non-conserved lncRNAs, conserved lncRNAs might have more exons, longer sequence length, higher expression levels, and lower tissue specificities. Functional annotation based on the A. thaliana coding-lncRNA gene co-expression network suggested potential functions of conserved lncRNAs including autophagy, locomotion, and cell cycle. Enrichment analysis revealed that the functions of conserved lncRNAs were closely related to the growth and development of the tissues in which they were specifically expressed. CONCLUSIONS: Comprehensive integration of large-scale lncRNA data and construction of a phylogenetic tree with orthologous lncRNA families from 25 flowering plants was used to provide an oversight of the evolutionary history of plant lncRNAs including origin, conservation, and orthologous relationships. Further analysis revealed a differential characteristic profile for conserved lncRNAs in A. thaliana when compared with non-conserved lncRNAs. We also examined tissue specific expression and the potential functional roles of conserved lncRNAs. The results presented here will further our understanding of plant lncRNA evolution, and provide the basis for further in-depth studies of their functions.


Assuntos
Magnoliopsida , RNA Longo não Codificante , Sequência Conservada , Redes Reguladoras de Genes , Filogenia , RNA Longo não Codificante/genética
7.
Appl Opt ; 60(24): 7466-7479, 2021 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-34613036

RESUMO

In recent years, image fusion has emerged as an important research field due to its various applications. Images acquired by different sensors have significant differences in feature representation due to the different imaging principles. Taking visible and infrared image fusion as an example, visible images contain abundant texture details with high spatial resolution. In contrast, infrared images can obtain clear target contour information according to the principle of thermal radiation, and work well in all day/night and all weather conditions. Most existing methods employ the same feature extraction algorithm to get the feature information from visible and infrared images, ignoring the differences among these images. Thus, this paper proposes what we believe to be a novel fusion method based on a multi-level image decomposition method and deep learning fusion strategy for multi-type images. In image decomposition, we not only utilize a multi-level extended approximate low-rank projection matrix learning decomposition method to extract salient feature information from both visible and infrared images, but also apply a multi-level guide filter decomposition method to obtain texture information in visible images. In image fusion, a novel fusion strategy based on a pretrained ResNet50 network is presented to fuse multi-level feature information from both visible and infrared images into corresponding multi-level fused feature information, so as to improve the quality of the final fused image. The proposed method is evaluated subjectively and objectively in a large number of experiments. The experimental results demonstrate that the proposed method exhibits better fusion performance than other existing methods.

8.
Theor Appl Genet ; 2021 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-34633473

RESUMO

bHLH family proteins play an important role in plant stress response. However, the molecular mechanism regulating the salt response of bHLH is largely unknown. This study aimed to investigate the function and regulating mechanism of the sweet sorghum SbbHLH85 during salt stress. The results showed that SbbHLH85 was different from its homologs in other species. Also, it was a new atypical bHLH transcription factor and a key gene for root development in sweet sorghum. The overexpression of SbbHLH85 resulted in significantly increased number and length of root hairs via ABA and auxin signaling pathways, increasing the absorption of Na+. Thus, SbbHLH85 plays a negative regulatory role in the salt tolerance of sorghum. We identified a potential interaction partner of SbbHLH85, which was phosphate transporter chaperone PHF1 and modulated the distribution of phosphate, through screening a yeast two-hybrid library. Both yeast two-hybrid and BiFC experiments confirmed the interaction between SbbHLH85 and PHF1. The overexpression of SbbHLH85 led to a decrease in the expression of PHF1 as well as the content of Pi. Based on these results, we suggested that the increase in the Na+ content and the decrease in the Pi content resulted in the salt sensitivity of transgenic sorghum.

9.
Sci Rep ; 11(1): 20166, 2021 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-34635752

RESUMO

Suppression-induced forgetting (SIF) refers to a memory impairment resulting from repeated attempts to stop the retrieval of unwanted memory associates. SIF has become established in the literature through a growing number of reports built upon the Think/No-Think (TNT) paradigm. Not all individuals and not all reported experiments yield reliable forgetting, however. Given the reliance on task instructions to motivate participants to suppress target memories, such inconsistencies in SIF may reasonably owe to differences in compliance or expectations as to whether they will again need to retrieve those items (on, say, a final test). We tested these possibilities on a large (N = 497) sample of TNT participants. In addition to successfully replicating SIF, we found that the magnitude of the effect was significantly and negatively correlated with participants' reported compliance during the No-Think trials. This pattern held true on both same- and independent-probe measures of forgetting, as well as when the analysis was conditionalized on initial learning. In contrast, test expectancy was not associated with SIF. Supporting previous intuition and more limited post-hoc examinations, this study provides robust evidence that a lack of compliance with No-Think instructions significantly compromises SIF. As such, it suggests that diminished effects in some studies may owe, at least in part, to non-compliance-a factor that should be carefully tracked and/or controlled. Motivated forgetting is possible, provided that one is sufficiently motivated and capable of following the task instructions.

10.
Front Cardiovasc Med ; 8: 739892, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34712710

RESUMO

Background: Cardiac magnetic resonance (CMR) has been shown to improve the diagnosis of myocarditis, but no systematic comparison of this technique is currently available. The purpose of this study was to compare the 2009 and 2018 Lake Louise Criteria (LLC) for the diagnosis of acute myocarditis using 3.0 T MRI with endomyocardial biopsy (EMB) as a reference and to provide the cutoff values for multiparametric CMR techniques. Methods: A total of 73 patients (32 ± 14 years, 71.2% men) with clinically suspected myocarditis undergoing EMB and CMR with 3.0 T were enrolled in the study. Patients were divided into two groups according to EMB results (EMB-positive and -negative groups). The CMR protocol consisted of cine-SSFP, T2 STIR, T2 mapping, early and late gadolinium enhancement (EGE, LGE), and pre- and post-contrast T1 mapping. Their potential diagnostic ability was assessed with receiver operating characteristic curves. Results: The myocardial T1 and T2 relaxation times were significantly higher in the EMB-positive group than in the EMB-negative group. Optimal cutoff values were 1,228 ms for T1 relaxation times and 58.5 ms for T2 relaxation times with sensitivities of 86.0 and 83.7% and specificities of 93.3 and 93.3%, respectively. The 2018 LLC had a better diagnostic performance than the 2009 LLC in terms of sensitivity, specificity, positive predictive value, and negative predictive value. T1 mapping + T2 mapping had the largest area under the curve (0.95) compared to other single or combined parameters (2018 LLC: 0.91; 2009 LLC: 0.76; T2 ratio: 0.71; EGEr: 0.67; LGE: 0.73; ). The diagnostic accuracy for the 2018 LLC was the highest (91.8%), followed by T1 mapping (89.0%) and T2 mapping (87.7%). Conclusion: Emerging technologies such as T1/ T2 mapping have significantly improved the diagnostic performance of CMR for the diagnosis of acute myocarditis. The 2018 LLC provided the overall best diagnostic performance in acute myocarditis compared to other single standard CMR parameters or combined parameters. There was no significant gain when 2018LLC is combined with the EGE sequence.

11.
Hum Brain Mapp ; 2021 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-34676650

RESUMO

Daydreaming and creativity have similar cognitive processes and neural basis. However, few empirical studies have examined the relationship between daydreaming and creativity using cognitive neuroscience methods. The present study explored the relationship between different types of daydreaming and creativity and their common neural basis. The behavioral results revealed that positive constructive daydreaming is positively related to creativity, while poor attentional control is negatively related to it. Machine learning framework was adopted to examine the predictive effect of daydreaming-related brain functional connectivity (FC) on creativity. The results demonstrated that task FCs related to positive constructive daydreaming and task FCs related to poor attentional control both predicted an individual's creativity score successfully. In addition, task FCs combining the positive constructive daydreaming and poor attentional control also had significant predictive effect on creativity score. Furthermore, predictive analysis based on resting-state FCs showed similar patterns. Both of the subscale-related FCs and combined FCs had significant predictive effect on creativity score. Further analysis showed the task and the resting-state FCs both mainly located in the default mode network, central executive network, salience network, and attention network. These results showed that daydreaming was closely related to creativity, as they shared common FC basis.

12.
J Immunother Cancer ; 9(10)2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34599020

RESUMO

BACKGROUND: T cell checkpoint immunotherapies have shown promising results in the clinic, but most patients remain non-responsive. CD47-signal regulatory protein alpha (SIRPα) myeloid checkpoint blockade has shown early clinical activity in hematologic malignancies. However, CD47 expression on peripheral blood limits αCD47 antibody selectivity and thus efficacy in solid tumors. METHODS: To improve the antibody selectivity and therapeutic window, we developed a novel affinity-tuned bispecific antibody targeting CD47 and programmed death-ligand 1 (PD-L1) to antagonize both innate and adaptive immune checkpoint pathways. This PD-L1-targeted CD47 bispecific antibody was designed with potent affinity for PD-L1 and moderate affinity for CD47 to achieve preferential binding on tumor and myeloid cells expressing PD-L1 in the tumor microenvironment (TME). RESULTS: The antibody design reduced binding on red blood cells and enhanced selectivity to the TME, improving the therapeutic window compared with αCD47 and its combination with αPD-L1 in syngeneic tumor models. Mechanistically, both myeloid and T cells were activated and contributed to antitumor activity of αCD47/PD-L1 bispecific antibody. Distinct from αCD47 and αPD-L1 monotherapies or combination therapies, single-cell RNA sequencing (scRNA-seq) and gene expression analysis revealed that the bispecific treatment resulted in unique innate activation, including pattern recognition receptor-mediated induction of type I interferon pathways and antigen presentation in dendritic cells and macrophage populations. Furthermore, treatment increased the Tcf7+ stem-like progenitor CD8 T cell population in the TME and promoted its differentiation to an effector-like state. Consistent with mouse data, the compounds were well tolerated and demonstrated robust myeloid and T cell activation in non-human primates (NHPs). Notably, RNA-seq analysis in NHPs provided evidence that the innate activation was mainly contributed by CD47-SIRPα but not PD-L1-PD-1 blockade from the bispecific antibody. CONCLUSION: These findings provide novel mechanistic insights into how myeloid and T cells can be uniquely modulated by the dual innate and adaptive checkpoint antibody and demonstrate its potential in clinical development (NCT04881045) to improve patient outcomes over current PD-(L)1 and CD47-targeted therapies.

13.
Nanoscale ; 13(34): 14417-14425, 2021 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-34473184

RESUMO

The recurrence and metastasis of tumor after surgery is the main cause of death for patients with breast cancer. Systemic chemotherapy suffered from low delivery efficiency to tumors and the side effects of chemo drugs. Localized chemotherapy using drug-containing implants is an alternative, while the reconstruction of breast tissue is generally considered after chemotherapy, resulting in a second surgery for patients. Here, we describe a strategy using implantable drug-containing polymeric scaffolds to deliver chemo drugs directly to the post-resection site, and simultaneously provide mechanical support and regenerative niche for breast tissue reconstruction. When doxorubicin was loaded in mesoporous silica nanoparticles and subsequently incorporated into polycaprolactone scaffolds (DMSN@PCL), a 9-week sustained drug release was achieved post implantation in mice. The local recurrence of residual tumor after surgery was significantly inhibited within 4 weeks in a post-surgical mouse model bearing xenograft MDA-MB-231 tumor. DMSN@PCL scaffolds exhibited good biocompatibility in mice during the treatment. We believe our strategy holds great promise as an adjuvant localized chemotherapy in clinics for combating post-resection breast cancer recurrence.


Assuntos
Antineoplásicos , Neoplasias da Mama , Nanopartículas , Animais , Antineoplásicos/uso terapêutico , Neoplasias da Mama/tratamento farmacológico , Linhagem Celular Tumoral , Doxorrubicina/uso terapêutico , Feminino , Humanos , Camundongos , Poliésteres
14.
Biomed Res Int ; 2021: 5473959, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34485514

RESUMO

Background: The hypercoagulable status, which forms a vicious cycle with hematogenous metastasis, is a common systemic alteration in cancers. As modeling is a key approach in research, a model which is suitable for studying how the hypercoagulable status promotes hematogenous metastasis in breast cancer is urgently needed. Methods: Based on the tumor-bearing period (TBP) and postoperative incubation period (PIP), 4T1-breast cancer models were constructed to evaluate coagulation and tumor burden to generate multiple linear regression-based lung metastasis prediction formula. Platelets and 4T1 cells were cocultured for 30 min or 24 h in vitro to evaluate the early and late phases of their crosstalk, and then the physical characteristics (concentration and size) and procoagulant activity of the coculture supernatants were assayed. Results: The multiple linear regression model was constructed as log10 (photon number) = 0.147 TBP + 0.14 PIP + 3.303 (TBP ≤ 25 and PIP ≤ 17) to predict lung metastasis. Coculture of platelets and 4T1 cells contributed to the release of extracellular vesicles (EVs) and the development of the hypercoagulable status. Conclusions: In vivo and in vitro hypercoagulable status models were developed to explore the mechanism of hypercoagulable status which is characterized by platelet activation and promotes hematogenous metastasis in breast cancer.


Assuntos
Neoplasias da Mama/sangue , Neoplasias da Mama/patologia , Neoplasias Hematológicas/sangue , Neoplasias Hematológicas/patologia , Trombofilia/sangue , Trombofilia/patologia , Animais , Linhagem Celular Tumoral , Modelos Animais de Doenças , Feminino , Camundongos , Camundongos Endogâmicos BALB C , Modelos Teóricos , Metástase Neoplásica , Ativação Plaquetária
15.
Biol Psychol ; 165: 108192, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34555480

RESUMO

Accumulating evidence indicates that structural and functional abnormalities in hippocampal formation are linked to major depressive disorder (MDD). However, the resting-state functional connectivity (RSFC) of hippocampal subfields in MDD remains unclear. This cross-sectional study aimed to investigate the RSFC of hippocampal subfields in a large sample of MDD patients. The results revealed that patients with MDD showed lower RSFC between the right anterior hippocampus and the insula, and the RSFC was inversely correlated with anxiety symptoms of depression. Depressed patients also showed decreased RSFC between the bilateral intermediate hippocampus and left nucleus accumbens (NAcc), and the hippocampus-NAcc circuit was negatively correlated with core symptoms of depression. The functional connectivity between the right anterior hippocampus and left postcentral gyrus increased with ageing in MDD patients compared with healthy controls. These findings suggest that the functional network of hippocampal subfields may underlie anxiety and core depression symptoms.


Assuntos
Transtorno Depressivo Maior , Adulto , Estudos Transversais , Transtorno Depressivo Maior/diagnóstico por imagem , Hipocampo/diagnóstico por imagem , Humanos , Longevidade , Imageamento por Ressonância Magnética
16.
Gastroenterology ; 2021 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-34536451

RESUMO

BACKGROUND & AIMS: G protein-coupled receptor (GPR) 120 has been implicated in regulating metabolic syndromes with anti-inflammatory function. However, the role of GPR120 in intestinal inflammation is unknown. Here, we investigated whether and how GPR120 regulates CD4+ T cell function to inhibit colitis development. METHODS: Dextran sodium sulfate (DSS)-induced colitis model, Citrobacter rodentium infection model, and CD4+ T cell adoptive transfer model were used to analyze the role of GPR120 in regulating colitis development. The effect of GPR120 on CD4+ T cell functions was analyzed by RNA sequencing, flow cytometry, and Seahorse metabolic assays. Mice were administered GPR120 agonist for investigating the potential of GPR120 agonist in preventing and treating colitis. RESULTS: Deficiency of GPR120 in CD4+ T cells resulted in more severe colitis in mice upon dextran sodium sulfate insult and enteric infection. Transfer of GPR120-deficient CD4+CD45Rbhi T cells induced more severe colitis in Rag-/- mice with lower intestinal interleukin (IL) 10+CD4+ T cells. Treatment with the GPR120 agonist CpdA promoted CD4+ T cell production of IL10 by up-regulating Blimp1 and enhancing glycolysis, which was regulated by mTOR. GPR120 agonist-treated wild-type, but not IL10-deficient and Blimp1-deficient, T helper 1 cells induced less severe colitis. Furthermore, oral administration of GPR120 agonist protected mice from intestinal inflammation in both prevention and treatment schemes. Gpr120 expression was positively correlated with Il10 expression in the human colonic mucosa, including patients with inflammatory bowel diseases. CONCLUSIONS: Our findings show the role of GPR120 in regulating intestinal CD4+ T cell production of IL10 to inhibit colitis development, which identifies GPR120 as a potential therapeutic target for treating inflammatory bowel diseases.

17.
Environ Pollut ; 291: 118094, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34517175

RESUMO

Indoor air quality ((IAQ) in classrooms was associated with the daily exposure of school-age children who are particularly vulnerable to air pollutants exposure, while few data exist to evaluate classroom indoor air quality nationwide in China. The subsample of the CIEHS 2018 study was performed in 66 classrooms of 22 primary schools nationwide in China. Temperature, relative humidity, PM2.5, PM10, CO2, CO, formaldehyde concentrations, bacteria and fungi were detected in all classrooms by using the instruments that meet the specified accuracy. The ratios of indoor to outdoor (I/O) of PM2.5 were calculated in each classroom to identify whether the indoor environment the pollutants comes from outdoors. The indoor PM2.5, PM10, CO, HCHO, bacteria and fungi GM concentration are 47.40 µg/m3, 72.91 µg/m3, 0.37 mg/m3, 0.02 mg/m3, 347.51 CFU/m3 and 362.76 CFU/m3, respectively. We observed that there were 66.5%, 52.6%, 22.4%, 1.8%, and 9.6% of the classrooms that exceeded the guideline values of PM2.5, PM10, CO2, HCHO, and bacteria, respectively. It should be attention that all of the classroom's PM2.5 concentrations in Shijiazhuang and Nanning, PM10 concentrations in Nanning, CO2 concentration in Lanzhou were exceeded the suggested values. Bacteria contamination in Shijiazhuang's classrooms is also serious. All classroom CO concentrations meet the requirement. The results indicated that classroom indoor PM2.5 was significantly positively correlated with indoor PM10 and CO2, while was negative correlated with temperature, CO, and fungi. Our results suggest that indoor air pollution in classrooms was a severe problem in Chinese primary schools. It is necessary to strengthen ventilation in the classroom to improve indoor air quality. What's more, a healthy learning environment should be created for primary school students.


Assuntos
Poluentes Atmosféricos , Poluição do Ar em Ambientes Fechados , Poluentes Atmosféricos/análise , Poluição do Ar em Ambientes Fechados/análise , Criança , China , Monitoramento Ambiental , Humanos , Material Particulado/análise , Instituições Acadêmicas
18.
DNA Cell Biol ; 40(10): 1317-1324, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34591648

RESUMO

Breast invasive carcinoma (BRCA) is becoming the most common malignant disease worldwide, and there is intense interest in identifying diagnostic biomarkers that can be targeted for treatment of BRCA. Recent evidence has shown that calcyclin binding protein (CacyBP) can function as either a tumor promoter or suppressor during carcinogenesis. Data in The Cancer Genome Atlas (TCGA) database show that CacyBP is overexpressed in human BRCA tissues, and high levels of CacyBP are associated with shorter overall survival. Immunohistochemical staining has shown that CacyBP levels are high in cancer tissue samples and associated with a higher likelihood of disease progression. We, therefore, conducted a knockout assay to determine the role of CacyBP in the development of BRCA. Knockout of CacyBP significantly inhibited MCF7 cell proliferation and colony formation. Apoptosis was higher in CacyBP knockout cells compared with control cells. Microarray analysis showed that the CacyBP knockout caused dysregulation of numerous genes closely related to ß-catenin signaling, whereas quantitative reverse-transcription PCR and immunoblotting showed that it to be inactivated. In summary, we conclude that when overexpressed, CacyBP acts as a potential oncogene for BRCA by regulating ß-catenin signaling.


Assuntos
Apoptose , Neoplasias da Mama/metabolismo , Proteínas de Ligação ao Cálcio/metabolismo , Carcinoma/metabolismo , Proliferação de Células , beta Catenina/metabolismo , Proteínas de Ligação ao Cálcio/genética , Feminino , Humanos , Células MCF-7 , Pessoa de Meia-Idade , Transdução de Sinais
19.
Radiology ; 301(3): 582-592, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-34519577

RESUMO

Background Assessment of subclinical myocardial dysfunction by using feature tracking has shown promise in prognosis evaluation of heart failure with preserved ejection fraction (HFpEF). Global early diastolic longitudinal strain rate (eGLSR) can identify earlier diastolic dysfunction; however, limited data are available on its prognostic value in HFpEF. Purpose To evaluate the association between left ventricular (LV) eGLSR and primary composite outcomes (all-cause death or heart failure hospitalization) in patients with HFpEF. Materials and Methods In this retrospective study, consecutive patients with HFpEF (included from January 2010 to March 2013) underwent cardiovascular MRI. The correlation between eGLSR and variables was assessed by using linear regression. The association between eGLSR (obtained with use of feature tracking) and outcomes was analyzed by using Cox proportional regression. Results A total of 186 patients with HFpEF (mean age ± standard deviation, 59 years ± 12; 77 women) were included. The eGLSR was weakly correlated with LV end-diastole volume index (Pearson correlation coefficient [r] = -0.35; P < .001), heart rate (r = 0.35; P < .001), and LV ejection fraction (r = 0.30; P < .001) and moderately correlated with LV end-systole volume index (r = -0.41; P < .001). At a median follow-up of 9.2 years (interquartile range, 8.7-10.0 years), 72 patients experienced primary composite outcomes. Impaired eGLSR, defined as an eGLSR of less than 0.57 per second, was associated with a greater rate of heart failure hospitalization or all-cause death (hazard ratio, 2.0 [95% CI: 1.1, 3.7]; P = .02) after adjusting for multiple clinical and imaging-based variables. Conclusion Left ventricular global early diastolic longitudinal strain rate obtained from cardiovascular MRI feature tracking was independently associated with adverse outcomes in patients with heart failure with preserved ejection fraction. © RSNA, 2021 Online supplemental material is available for this article. An earlier incorrect version appeared online. This article was corrected on October 22, 2021.

20.
Am J Transl Res ; 13(7): 8102-8109, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34377293

RESUMO

OBJECTIVE: To explore the clinical treatment outcomes and parental satisfaction of children with diarrhea receiving comprehensive nursing intervention. METHODS: A total of 128 diarrheic children treated in our hospital from June 2016 to June 2017 were recruited and divided into a control group (n=64) receiving conventional nursing and an observation group (n=64) receiving comprehensive nursing intervention, as per a random number table. The clinical outcomes, electrolyte disorders, recovery of gastrointestinal function, quality of life and parental satisfaction were compared between the two groups after nursing. RESULTS: The overall response rate of the observation group was higher than that of the control group (81.25% vs. 51.56%) (P < 0.05). Nursing satisfaction in the observation group was higher than that in the control group (100.00% vs. 75.00%) (P < 0.05). However, the time to resolution of clinical symptoms and time to return of bowel sounds in the observation group were shorter than those in the control group (P < 0.05). After nursing, the incidence of electrolyte disorders in the observation group was lower than that in the control group. The scores of physical symptoms, physical functioning, emotional functioning, cognitive functioning and social functioning in PedsQLTM Measurement Model were higher than those in the control group, and the electrolyte index monitoring results were better than those in the control group (P < 0.05). CONCLUSION: Comprehensive nursing intervention can effectively reduce the incidence of electrolyte disorders, improve parental satisfaction, accelerate the recovery of gastrointestinal function and improve the quality of life in the treatment of pediatric diarrhea.

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