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1.
Bioorg Med Chem ; 27(22): 115109, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31615696

RESUMO

A series of structurally diverse chrysin-chromene-spirooxindole hybrids were designed, synthesized via a Knoevenagel/Michael/cyclization of chrysin and isatylidene malononitrile derivatives through utilizing a hybrid pharmacophore approach. The newly synthesized compounds were evaluated for their in vitro anticancer activity, and most of the compounds showed stronger anti-proliferative activity than parent compound chrysin. In particular, compound 3e had the highest cytotoxicity towards A549 cells (IC50 = 3.15 ±â€¯0.51 µM), and had better selectivity in A549 cells and normal MRC-5 cells. Furthermore, compound 3e could significantly inhibit the proliferation and migration of A549 cells in a dose-dependent manner, as well as induce the apoptosis possibly through mitochondria-mediated caspase-3/8/9 activation and multi-target co-regulation of the p53 signaling pathway. Thus, our results provide in vitro evidence that compound 3e may be a potential candidate for the development of new anti-tumour drugs.

2.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 35(8): 714-720, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31638569

RESUMO

Objective To investigate the effect of progranulin (PGRN) on osteoporosis in ovariectomized mice. Methods PGRN-knockout (PGRN-/-) and wild-type mice were ovariectomized to induce postmenopausal osteoporosis models. Next, the bone tissues in all mice were scanned by Micro-CT and three-dimensional reconstruction was performed to detect the micro-structure, followed by trabecula data analysis. The morphology and osteoclasts in the bone tissues of PGRN-/- and wild-type mice were observed by HE staining and TRAP staining, respectively. The expression of receptor activator for nuclear factor-κB ligand (RANKL), tumor necrosis factor α (TNF-α) and P65 were detected by immunohistochemistry. The expression of TRAP mRNA in the mice was measured using fluorescence quantitative PCR and the protein expression of MMP9, MMP14, P65 was detected by Western blot analysis. Results Bone mineral density (BMD), bone volume fraction (BV/TV), trabecular number (Tb.N) and trabecular thickness (Tb.Th) in the PGRN-/- group were significantly higher than those in the wild-type group, while the trabecular separation (Tb.S) in the PGRN-/- group was in the contrary. The degree of osteoporosis was less severe and number of osteoclasts in the PGRN-/- mice were reduced, likewise, RANKL, TNF-α, MMP9, MMP14 and P65 as well as TRAP mRNA were down-regulated in the PGRN-/- group compared with the wild-type group. Conclusion PGRN aggravates the postmenopausal osteoporosis in ovariectomized mice.

3.
Bioorg Med Chem Lett ; 29(19): 126604, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31445854

RESUMO

This manuscript describes the discovery of a series of macrocyclic inhibitors of FXIa with oral bioavailability. Assisted by structure based drug design and ligand bound X-ray crystal structures, the group linking the P1 moiety to the macrocyclic core was modified with the goal of reducing H-bond donors to improve pharmacokinetic performance versus 9. This effort resulted in the discovery of several cyclic P1 linkers, exemplified by 10, that are constrained mimics of the bioactive conformation displayed by the acrylamide linker of 9. These cyclic P1 linkers demonstrated enhanced bioavailability and improved potency.

4.
FASEB J ; 33(11): 12036-12046, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31365830

RESUMO

Uremic pruritus with elevated levels of calcium phosphate (CaP) in skin is a common symptom in patients with chronic kidney disease (CKD). In this study, we demonstrate that intradermal injection of CaP into mice triggered scratching by up-regulating the IL-6 in skin and phosphorylation of ERKs in dorsal root ganglion (DRG) in a dose-dependent manner. IL-6 is essential because the CaP-induced up-regulation of phosphorylated (p)-ERK in DRG was considerably reduced in the IL-6 knockout mice. Microarray analysis in conjunction with real-time PCR revealed a higher mRNA expression of Bruton's tyrosine kinase (BTK) gene in DRG after CaP injection. The inhibition of BTK by ibrutinib noticeably diminish the CaP-induced up-regulation of IL-6 and p-ERK in mice. A high amount of IL-6 was detected in itchy skin and blood of patients with CKD. The expressions of p-BTK and p-ERK in DRG primary cells reached maximum levels at 1 and 10 min, respectively, after treatment of recombinant IL-6 and were significantly reduced by treatment of IL-6 along with ibrutinib. The mechanism by which the CaP-induced pruritus mediated by the IL-6/p-BTK/p-ERK signaling was revealed.-Keshari, S., Sipayung, A. D., Hsieh, C.-C., Su, L.-J., Chiang, Y.-R., Chang, H.-C., Yang, W.-C., Chuang, T.-H., Chen, C.-L., Huang, C.-M. IL-6/p-BTK/p-ERK signaling mediates calcium phosphate-induced pruritus.

5.
ACS Appl Mater Interfaces ; 11(29): 26355-26363, 2019 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-31260241

RESUMO

Covalent organic frameworks (COFs) with redox-active units are a class of ideal materials for electrochemical-energy-storage devices. A novel two-dimensional (2D) PDC-MA-COF with redox-active triazine units was prepared via aldehyde-amine condensation reaction by using 1,4-piperazinedicarboxaldehyde (PDC) and melamine (MA) as structural units, which possessed high specific surface area (SBET = 748.2 m2 g-1), narrow pore width (1.9 nm), large pore volume (1.21 cm3 g-1), and high nitrogen content (47.87%), for pseudocapacitance application. The interlayer C-H···N hydrogen bonding can "lock" the relative distance between two adjacent layers to avoid an interlayer slip, which is more conducive to maintaining the ordered pore structure of the COF and improving a fast charge transfer between the electrode interface and triazine units. The PDC-MA-COF exhibited an excellent electrochemical performance with the highest specific capacitance of 335 F g-1 along with 19.71% accessibility of the redox-active triazine units in a three-electrode system and 94 F g-1 in a two-electrode system at 1.0 A g-1 current density. Asymmetric supercapacitor of PDC-MA-COF//AC assembled using PDC-MA-COF and activated carbon (AC) as positive and negative electrode materials, respectively, exhibited a high energy density of 29.2 W h kg-1 with a power density of 750 W kg-1. At the same time, it also showed an excellent cyclic stability and could retain 88% of the initial capacitance after 20 000 charge-discharge cycles, which was better than those of the most of the analogous materials reported previously. This study provided a new strategy for designing redox-active COFs for pseudocapacitive storage.

6.
Org Biomol Chem ; 17(28): 6757-6761, 2019 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-31259341

RESUMO

Iodine catalysed the transfer hydrogenation of a benzylic C-C σ-bond in [2.2]paracyclophane with water to yield 4,4'-dimethylbibenzyl. The C-C σ-bond was first cleaved by homolytic substitution with iodine radicals to produce a 4,4'-diiodomethylbibenzyl intermediate. The benzylic C-I bonds in this intermediate were subsequently reduced by HI, generated in situ from the disproportionation of I2 with H2O, to achieve transfer hydrogenation and regenerate I2.

7.
Org Lett ; 21(14): 5514-5518, 2019 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-31251635

RESUMO

An organocatalytic asymmetric arylmethylation/N-hemiacetalization of 2-indolyl methane derivatives and 2-enals was developed. Notably, the 2-methyl of indole was readily deprotonated to produce highly reactive nucleophilic species by introducing the nitro group at the C3 position of the indole ring. A spectrum of valuable chiral dihydropyrido[1,2-a]indoles were efficiently constructed with excellent enantioselectivity (up to >99% ee). Furthermore, the corresponding products could be easily functionalized via simple deprotonation and treatment with other electrophiles with excellent diastereoselectivities (>20:1 dr).

8.
Chem Commun (Camb) ; 55(51): 7346-7349, 2019 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-31169844

RESUMO

An efficient catalytic asymmetric 1,3-dipolar cycloaddition of azomethine ylides with four-membered ring-containing exocyclic alkenes has been developed, and either the exo or endo spirocyclic pyrrolidine-azetidine/oxe(thie)tane derivatives were diastereodivergently generated by employing Cu(i)/tBu-Phosferrox and a Cu(i)/N,O-ligand complex, respectively. Notably, various heteroatom-containing (N, O, S) exocyclic alkenes were found to be well-tolerated in this transformation.

9.
BMC Neurol ; 19(1): 108, 2019 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-31146703

RESUMO

BACKGROUND: The study is intended to fill the knowledge gap about the neuropsychology and neuromotor developmental outcomes, and identify the perinatal risk factors for late preterm infants (LPIs 34~36 weeks GA) born with uncomplicated vaginal birth at the age of 24 to 30 months. METHODS: The parents/guardians of 102 late preterm infants and 153 term infants, from 14 community health centers participated in this study. The Modified Checklist for Autism in Toddlers (M-CHAT) questionnaire, the Chinese version of Gesell Development Diagnosis Scale (GDDS), and the Sensory Integration Schedule (SIS), a neurological examination for motor disorders (MD) were carried out. Infants screening positive to the M-CHAT were referred to specialist autism clinics. RESULTS: Forty-six LPIs (45.1%) scored low in GDDS. Nine LPIs (8.8%) scored positive on M-Chat. 8.8% of LPIs (9 out of 102) were diagnosed MD (p <  0.05). Compared with their full-term peers, LPIs had statistically lower scores in GDDS and the Child Sensory Integration Checklist. LPIs who had positive results on M-CHAT showed unbalanced abilities in every part of GDDS. Risk factors of twin pregnancies, pregnancy induced hypertension and premature rupture of membranes had negative correlation with GDDS (all p <  0.05). Birth weight and gestational age were positively correlated with GDDS. CONCLUSIONS: LPIs shall be given special attention as compared to normal deliveries, as they are at increased risk of neurodevelopment impairment, despite being born with no major problems. Some perinatal factors such as twin pregnancies, and pregnancy induced hypertension etc. have negative effects on their neurodevelopment. Regular neurodevelopmental follow- up and early intervention can benefit their long term outcomes.


Assuntos
Deficiências do Desenvolvimento/epidemiologia , Deficiências do Desenvolvimento/etiologia , Recém-Nascido Prematuro , Nascimento Prematuro , Pré-Escolar , China , Feminino , Idade Gestacional , Humanos , Masculino , Exame Neurológico , Inquéritos e Questionários
10.
Huan Jing Ke Xue ; 40(3): 1475-1482, 2019 Mar 08.
Artigo em Chinês | MEDLINE | ID: mdl-31088000

RESUMO

Rhizosphere and non-rhizosphere soil samples under different long-term fertilization treatments including control without fertilizer (CK), chemical fertilization alone (NPK), rice residues combined with NPK (NPKS), 30% manure plus 70% chemical fertilizers (LOM), and 60% manure plus 40% chemical fertilizers (HOM) were collected from a paddy field in a red soil hilly area in Ningxiang City, Hunan Province, China. The characteristics of microbial carbon utilization in the soils were studied. Results of 18O-H2O tracer analysis showed that both soil microbial biomass carbon content (MBC) and microbial growth rate (CGrowth) were highest in the HOM treatment, whereas they were lowest in CK. In the rhizosphere soil, the highest basal respiration was observed in HOM, and the lowest values were in CK and NPK. Microbial carbon utilization efficiency (CUE) was highest in NPK but lowest in the LOM and HOM treatments. In non-rhizosphere soil, no significant differences between basal respiration and CUE were observed among the fertilization treatments. Results from MicroRespTM showed that the ability of microorganisms to metabolize exogenous carbon sources was higher in non-rhizosphere soil than in rhizosphere soil. The application of organic materials (rice residues or manure) increased the microbial metabolic rate of carboxylic acids, amino acids, and carbohydrates in the order carboxylic acids > amino acids and carbohydrates > complex compounds. Redundancy analysis of the microbial metabolism patterns of various carbon substrates showed that:① CK was well separated from the fertilization treatments; ② NPK was grouped with NPKS, whereas LOM and HOM were grouped together and were separate from NPK and NPKS. This indicates that the fertilization treatments changed the microbial carbon metabolism patterns. The above-mentioned results indicated that the fertilization treatments did not affect microbial CUE and basal respiration. However, exogenous carbon source input (such as root exudates) and the application of organic materials can increase microbial basal respiration, and thus, reduce microbial CUE.


Assuntos
Carbono/química , Metaboloma , Oryza , Rizosfera , Microbiologia do Solo , Solo/química , China , Fertilizantes , Esterco
11.
Environ Sci Pollut Res Int ; 26(21): 21915-21924, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31140087

RESUMO

Ozonation treatment of municipal secondary effluent is complicated by the low solubility of ozone and inefficient production of hydroxyl free radicals from ozone decomposition. To resolve these problems, this study investigated methods for intensifying ozonation treatment, using a combination of microbubbles and ultraviolet (UV) irradiation (UV/MBO). The high efficiency of the method was illustrated by treating river water containing refractory components derived from secondary effluent in a wastewater treatment plant. The results showed that the ozone mass transfer coefficient in a microbubble system was an order of magnitude compared with a conventional macrobubble system at the initial stage. The amount of ·OH generated during the treatment was quantified using a fluorescent probe analysis. The amount of ·OH in the UV/MBO system was almost 2-6 times more than the amount found with conventional ozonation using macrobubbles (CO), CO with UV irradiation (UV/CO), and microbubble ozonation (MBO) units. The UV/MBO system achieved chemical oxygen demand (COD), UV254, and UV400 removal performance rates of up to 37.50%, 81.15%, and 94.74% respectively. These levels were 2-36% higher than those in other systems. The coupling UV/MBO treatment significantly reduced all five categories of substances according to EEM spectra and fluorescence regional integration. The distribution of fractions with different molecular weights (MW) was altered and the UV254 of MW (< 500 Da) increased by 15.8%. The biodegradability of the water was significantly improved, as indicated by the TOC/UV254. This is ascribed to the enhanced degradation of refractory organics in the water. The combination of the UV/microbubble technique with ozonation could provide an efficient approach for advanced wastewater treatment. Graphical abstract.


Assuntos
Microbolhas , Raios Ultravioleta , Eliminação de Resíduos Líquidos/métodos , Biodegradação Ambiental , Análise da Demanda Biológica de Oxigênio , Peso Molecular , Ozônio/química , Águas Residuárias/química , Poluentes Químicos da Água/química
12.
Lipids Health Dis ; 18(1): 108, 2019 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-31077212

RESUMO

BACKGROUND: This study aims to investigate the effect of lipid metabolism disorder on liver function in patients with malignant tumors after chemotherapy. METHOD: A total of 428 patients with malignant tumors with normal liver function in our hospital between May 2013 to June 2018 were divided into an observation group (lipid metabolism disorder, n = 265) and control group (normal lipid metabolism, n = 163). The lipid metabolism levels and liver damage of the two groups were compared before and after chemotherapy. RESULTS: No significant differences in age, gender, body mass index, tumor types, history of surgery, levels of alanine aminotransferase (ALT; an indicator of liver function), and chemotherapy regimen were observed between the two groups. However, the observation group showed increased levels of total cholesterol (P = 0.000), triglycerides (P = 0.000), and low-density lipoprotein (P = 0.01), as well as decreased levels of high-density lipoprotein (P = 0.000) before chemotherapy compared with the control group. Furthermore, patients with lipid metabolism disorders were more likely to develop abnormal liver function after chemotherapy. Moreover, mixed lipid metabolism disorder was more likely to cause severe liver damage after chemotherapy. Additionally, the number of patients with lipid metabolism disorders after chemotherapy (n = 367) was significantly increased compared with before chemotherapy (n = 265) (P < 0.01), indicating that chemotherapy might induce or aggravate an abnormal lipid metabolism. CONCLUSIONS: After receiving chemotherapy, patients with malignant tumors presenting lipid metabolism disorders are more prone to liver damage and lipid metabolism disorders than patients with a normal lipid metabolism.


Assuntos
Metabolismo dos Lipídeos , Fígado/fisiopatologia , Neoplasias/tratamento farmacológico , Neoplasias/metabolismo , Estudos de Casos e Controles , Feminino , Humanos , Lipídeos/sangue , Fígado/patologia , Masculino , Pessoa de Meia-Idade , Neoplasias/sangue , Neoplasias/fisiopatologia
13.
Huan Jing Ke Xue ; 40(3): 1483-1490, 2019 Mar 08.
Artigo em Chinês | MEDLINE | ID: mdl-31088001

RESUMO

Available carbon is the most active part of the soil carbon pool. It is also the main carbon source of soil microbes and plays an important role in the processes of soil organic carbon mineralization and accumulation. However, the mechanisms are still not clear how soil organic carbon mineralization and its priming effect (PE) are affected by different input levels of readily available carbon, based on the growth requirements of microbes in paddy soil. In this study, an incubation experiment was conducted by adding different levels (0.5, 1, 3, and 5 times of MBC) of exogenous source organic carbon (13C-glucose) to the soil. The mineralization dynamics of labile organic carbon and its priming effect was investigated. The mineralization rate of glucose-C increased significantly with the increasing carbon loading level. The distribution of glucose-C into rapid and slow C pools was also exponentially correlated with the carbon loading (R2=0.99, P<0.05 and R2=0.99, P<0.05, respectively). Negative PE was observed at high carbon loading (3×MBC and 5×MBC); while positive PE was induced by low carbon loading (0.5×MBC and 1×MBC). The cumulative PE was 160.0 mg·kg-1 and 325.1 mg·kg-1, respectively, at the end of the incubation. Redundancy analysis showed that the main factors affecting the cumulative PE were MBC, MBN, and DOC at the initial glucose mineralization stage, while ß-glucosidase, chitinase, and ammonium nitrogen were the main factors at later stages. Therefore, the readily available carbon loading has an important effect on the organic carbon mineralization and PE in paddy soil. Higher carbon loading was good for the accumulation of organic carbon sequestration in paddy soil. This study is of great scientific significance for revealing the activity of organic carbon in paddy fields and for its contribution to the development of sustainable agriculture.

14.
Zhongguo Zhong Yao Za Zhi ; 44(6): 1110-1118, 2019 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-30989972

RESUMO

Chinese herbal medicines( CHMs) are a class of preparations made from natural plants that pose health beneficial properties as well as illness prevention functions. Thanks to a panel of salutary features,such as comprehensive immunological enhancement and inhibition of pathogenic bacteria,negligible side-effects,inappreciable drug-resistance,CHMs have been taken as one of the costeffective candidates for antibiotics substitutions. Through probiotics fermentation,the enzymatic hydrolysis of matrixes of CHMs enables easier release of the active ingredient as well as endows less toxicity of the preparations derived. During fermentation,the macromolecule or polymers forms of the active ingredient can be cut down to smaller molecule,which favors the transmembrane transport and improve adsorption of the active ingredients by the tissues. Other than the enzymatic benefits,probiotics can produce metabolites that inhibit pathogenic bacteria propagation,which may function synergically with the inhibitory effects of the CHMs preparations to fight the target pathogens. In addition,the oligosaccharide like components of CHMs can promote the growth of probiotics in intestinal environment which may largely facilitate the gut health. To summarize,the fermentation of CHMs using probiotics brings about the biochemical reactions and elevates the health beneficial effects by synergy of the microbial and herbal activities. It has been proved to be one of promising approaches as to antibiotic substitutions,particularly in livestock and poultry breeding industries. This review covered the recent progress of CHMs fermentation on the aspects of microbial strains,patterns of fermentation and active substances from fermentation of CHMs and their potency,respectively.


Assuntos
Efeitos Colaterais e Reações Adversas Relacionados a Medicamentos , Medicamentos de Ervas Chinesas , Fermentação , Humanos , Pesquisa
15.
Bioorg Med Chem Lett ; 29(11): 1291-1297, 2019 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-30962085

RESUMO

Hepatitis B virus (HBV) is a global health problem requiring more efficient and better tolerated anti-HBV agent. In this paper, a series of novel 2'-deoxy-2'-fluoro-2'-C-methyl-ß-d-arabinofuranosyl 8-azanebularine analogues (1 and 2a) and N4-substituted 8-azaadenosine derivatives (2b-g) were designed, synthesized and screened for in vitro anti-HBV activity. Two concise and practical synthetic routes were developed toward the structural motif construction of 2'-deoxy-2'-fluoro-2'-C-methyl-ß-d-arabinofuranosyl 8-azainosine from the ribonolactone 3 under mild conditions. The in vitro anti-HBV screening results showed that these 8-azanebularine analogues had a significant inhibitory effect on the expression of HBV antigens and HBV DNA at a concentration of 20 µM. Among them, halogen-substituted 8-azaadenosine derivative 2g displayed activities comparable to that of 3TC. In particular, 2g retained excellent activity against lamivudine-resistant HBV mutants.

16.
Ying Yong Sheng Tai Xue Bao ; 30(1): 189-197, 2019 Jan 20.
Artigo em Chinês | MEDLINE | ID: mdl-30907540

RESUMO

Soil samples were collected from paddy ecosystem under five long-term fertilization treatments, including control without fertilizer (CK), chemical fertilization alone (NPK), rice residue combined with NPK (NPKS), 30% manure plus 70% chemical fertilizer (LOM), and 60% manure plus 40% chemical fertilizer (HOM) in Ningxiang City, Hunan Province. The cha-racteristics of amino sugars accumulation in the rhizosphere and non-rhizosphere soils at rice tillering stage were analyzed. Results showed that the contents of soil organic carbon, total amino sugars and three amino monosaccharides (muramic acid, glucosamine and galactosamine) with long-term application of organic materials (rice residue or manure) were significantly higher compared with CK and NPK. The inconsistent accumulation trends of the three amino monosaccharides under different fertilization treatments indicated that different responses of microbial groups to various fertilization treatments. The content of total amino sugars was not significantly different between the rhizosphere soil and the non-rhizosphere soil, probably because the agricultural operations such as plowing could homogenize paddy soils. The contribution of amino sugar derived carbon to soil organic carbon ranged from 24.0 to 28.3 mg·g-1, which was highest in NPKS, and lowest in HOM and CK. The ratio of fungal to bacterial residues (fungal glucosamine/muramic acid) ranged from 24.4 to 36.6, indicating that fungi dominated the degradation and transformation of organic matter in all the soils. Compared with that under NPK and CK, the participation of organic matter transformation from fungi under NPKS treatment was increased, whereas the bacteria involved in organic matter transformation under HOM treatment was enhanced.


Assuntos
Amino Açúcares/metabolismo , Fertilizantes , Oryza/fisiologia , Rizosfera , Solo
17.
Anal Bioanal Chem ; 411(12): 2607-2617, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30877344

RESUMO

A novel and sensitive fluorescent sensor was synthesized for the rapid and specific recognition of aflatoxin B1 (AFB1) by our combining molecular imprinting techniques with quantum dot technology. Molecularly imprinted polymers coated CdTe quantum dots (MIP@CdTe QDs) were prepared through the Stöber method with 5,7-dimethoxycoumarin as a dummy template. 3-Aminopropyltriethoxysilane was selected as the functional monomer, and tetraethyl orthosilicate was used as the cross-linking agent. The best molar ratio of 5,7-dimethoxycoumarin to functional monomer to cross-linker was 4:20:15. The MIP@CdTe QD composites were characterized by Fourier transform infrared spectroscopy, transmission electron microscopy, and fluorescence spectroscopy. Under the optimum conditions, the relative fluorescence intensity of the MIP@CdTe QDs showed adequate linearity with AFB1 concentration over the range from 80 to 400 ng/g. The detection limit is 4 ng/g, according to 3s/K. Finally, the method was successfully applied to the quantitative determination of AFB1 in real samples. The spike recoveries at different spiking levels ranged from 99.20% to 101.78%, which were consistent with those measured by ultrahigh-performance liquid chromatography-mass spectrometry. The method developed for AFB1 detection lays the foundation for rapid detection of trace amounts of other exogenous harmful substances in a complicated matrix.


Assuntos
Aflatoxina B1/análise , Compostos de Cádmio/química , Impressão Molecular , Polímeros/química , Pontos Quânticos/química , Telúrio/química , Limite de Detecção , Microscopia Eletrônica de Transmissão/métodos , Espectrometria de Fluorescência/métodos , Espectrofotometria Ultravioleta , Espectroscopia de Infravermelho com Transformada de Fourier/métodos
18.
ACS Appl Mater Interfaces ; 11(14): 13676-13684, 2019 Apr 10.
Artigo em Inglês | MEDLINE | ID: mdl-30888150

RESUMO

In terms of detection of antibiotics within complex media, the nonspecific adsorption is an enormous challenge and antifouling sensing interfaces capable of reducing the nonspecific adsorption from complex biological samples are highly desirable. In this work, a novel antifouling electrochemical immunosensor was explored based on the self-assembly of two kinds of poly( N-isopropylacrylamide) microgels on the surface of graphene oxide for sensitive detection of streptomycin (STR). The microgels modified with glycidyl methacrylate (GMA) and zwitterionic liquid 1-propyl-3-vinylimidazole sulfonate (PVIS) were prepared. The microgels with GMA were used by combining specific recognition of anti-STR. The rapid specific binding of antigen and anti-STR resulted in a decrease of current density to generate electrochemical responsive signals. Zwitterionic liquid-modified microgels were used for antifouling, which can form stronger hydration and show excellent antifouling ability. As a result, we achieved efficient and sensitive detection of STR in the complex sample with evidently resisted nonspecific adsorption effect, the wide linear range toward STR was from 0.05 to 100 ng mL-1, with a detection limit down to 1.7 pg mL-1. The immunosensor based on the surface functionalization of microgels showed promising applications for the detection of antibiotics in complex media.


Assuntos
Técnicas Biossensoriais , Técnicas Eletroquímicas , Estreptomicina/isolamento & purificação , Adsorção , Compostos de Epóxi/química , Ouro/química , Metacrilatos/química , Estreptomicina/química
19.
Am J Physiol Renal Physiol ; 316(6): F1094-F1102, 2019 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-30892932

RESUMO

The incidence of urothelial carcinoma (UC) is higher in patients undergoing chronic dialysis than in the general population. This study investigated plasma miRNA profiling as the ancillary diagnosis biomarker associated with UC in patients undergoing chronic hemodialysis. We successfully screened out and detected miRNA expression from plasma in eight patients undergoing dialysis through quantitative real-time PCR array analysis and identified eight candidate miRNAs. The candidate miRNAs were then validated using single quantitative RT-PCR assays from 52 plasma samples. The miRNA classifier for ancillary UC detection was developed by multiple logistic regression analyses. Moreover, we validated the classifier by testing another nine samples. Expression levels of miR-150-5p, miR-150-5p/miR-155-5p, miR-378a-3p/miR-150-5p, miR-636/miR-150-5p, miR-150-5p/miR-210-3p, and miR-19b-1-5p/miR-378a-3p were shown to be significantly different between UC and non-UC samples (P = 0.035, 0.0048, 0.016, 0.024, 0.038, and 0.048). Kaplan-Meier curve analysis also showed that low miR-19b-1-5p expression was associated with a worse prognosis (P = 0.0382). We also developed a miRNA classifier based on five miRNA expression levels to predict UC and found that the area under curve was 0.882. The classifier had a sensitivity of 80% (95% confidence interval: 0.5191% to 0.9567%) and a specificity of 83.7% (95% confidence interval: 0.6799% to 0.9381%). This classifier was tested by nine samples with 100% accuracy. The miRNA classifier offers higher sensitivity and specificity than the existing makers. Thus, this approach will improve the prospective diagnosis of UC in patients undergoing chronic hemodialysis.

20.
Sci Rep ; 9(1): 3440, 2019 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-30837628

RESUMO

Blast lung injury (BLI) caused by both military and civilian explosions has become the main cause of death for blast injury patients. By building three-dimensional (3D) models of rat explosion regions, we simulated the surface pressure of the skin and lung. The pressure distributions were performed at 5 distances from the detonation center to the center of the rat. When the distances were 40 cm, 50 cm, 60 cm, 70 cm and 80 cm, the maximum pressure of the body surface were 634.77kPa, 362.46kPa, 248.11kPa, 182.13kPa and 109.29kPa and the surfaces lung pressure ranges were 928-2916 Pa, 733-2254 Pa, 488-1236 Pa, 357-1189 Pa and 314-992 Pa. After setting 6 virtual points placed on the surface of each lung lobe model, simulated pressure measurement and corresponding pathological autopsies were then conducted to validate the accuracy of the modeling. For the both sides of the lung, when the distance were 40 cm, 50 cm and 60 cm, the Pearson's values showed strong correlations. When the distances were 70 cm and 80 cm, the Pearson's values showed weak linear correlations. This computational simulation provided dynamic anatomy as well as functional and biomechanical information.

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