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1.
Oxid Med Cell Longev ; 2022: 6232902, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35265262

RESUMO

Mitochondria can supply adenosine triphosphate (ATP) to the tissue, which can regulate metabolism during the pathologic process and is also involved in the pathophysiology of neuronal injury after stroke. Recent studies have suggested that selective autophagy could play important roles in the pathophysiological process of stroke, especially mitophagy. It is usually mediated by the PINK1/Parkin-independent pathway or PINK1/Parkin-dependent pathway. Moreover, mitophagy may be a potential target in the therapy of stroke because the control of mitophagy is neuroprotective in stroke in vitro and in vivo. In this review, we briefly summarize recent researches in mitophagy, introduce the role of mitophagy in the pathogenesis of stroke, then highlight the strategies targeting mitophagy in the treatment of stroke, and finally propose several issues in the treatment of stroke by targeting mitophagy.


Assuntos
Mitofagia/genética , Acidente Vascular Cerebral/patologia , Humanos
2.
Phytomedicine ; 93: 153765, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34610527

RESUMO

BACKGROUND: Hyperuricemia (HUA) is an important risk factor for gout, renal dysfunction and cardiovascular diseases. The whole plant of Persicaria capitata (Buch.-Ham. ex D. Don) H. Gross, namely Persicaria capitata herba, is a well-known ethnic herb with potent therapeutic effects on urinary tract infections and urinary calculus, yet previous reports have only focused on its effect on urinary tract infections. PURPOSE: To evaluate the therapeutic potential of P. capitata herba against gout by investigating its antihyperuricemia and antigouty arthritis effects and possible mechanisms. METHODS: The ethanol extract (EP) and water extract (WP) of P. capitata herba were prepared by extracting dried and ground whole plants of P. capitata with 75% ethanol and water, respectively, followed by removal of solvents and characterization by UHPLC-Q-TOF/MS. The antihyperuricemia and antigouty arthritis effects of the two extracts were evaluated in a potassium oxonate- and hypoxanthine-induced hyperuricemia mouse model and a monosodium urate crystal (MSUC)-induced acute gouty arthritis mouse model, respectively. The mechanisms were investigated by testing their effects on the expression of correlated proteins (by Western blot) and mRNAs (by RT-PCR). RESULTS: UHPLC-HRMS fingerprinting and two chemical markers (i.e., quercetin and quercitrin) determination were used for the characterization of the WP and EP extracts. Both WP and EP extracts showed pronounced antihyperuricemia activities, with a remarkable decline in serum uric acid and a marked increase in urine uric acid in hyperuricemic mice. Unlike the clinical xanthine oxidase (XOD) inhibitor allopurinol, WP and EP did not show any distinct renal toxicities. The underlying antihyperuricemia mechanism involves the inhibition of the activity and expression of XOD and the downregulation of the mRNA and protein expression of glucose transporter 9 (GLUT9) and urate transporter 1 (URAT1). The extracts of P. capitata herba also demonstrated remarkable anti-inflammatory activity in MSUC-induced acute gouty arthritis mice. The mechanism might involve inhibitory effects on the expression of proinflammatory factors. CONCLUSIONS: The extracts of P. capitata herba possessed pronounced antihyperuricemia and antigouty arthritis effects and were, therefore, promising natural medicines for hyperuricemia-related disorders and gouty arthritis. The use of P. capitata herba for the treatment of urinary calculus may be, at least to some degree, related to its potential as an antihyperuricemia and antigouty arthritis drug.


Assuntos
Artrite Gotosa , Hiperuricemia , Animais , Artrite Gotosa/tratamento farmacológico , Hiperuricemia/induzido quimicamente , Hiperuricemia/tratamento farmacológico , Camundongos , Ácido Oxônico , Extratos Vegetais/farmacologia , Ácido Úrico , Xantina Oxidase
3.
Nat Prod Res ; 35(9): 1445-1451, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-31460795

RESUMO

Two new dihydroisocoumarins (1 and 2), together with six known compounds (3-8), were isolated from the fungus Penicillium sp. XR046 collected from the Xinren coal area of Guizhou province in China. Their structures were elucidated on the basis of spectroscopic analysis. The absolute configurations of C-3 in 1 and 2 were established by comparison of their CD data with those of known compounds. Compounds 1-6 showed anti-microbial activities with MIC values in the range of 50∼100 µg/mL against Candida albicans, Staphylococcus epidermidis, Bacillus subtilis, and Escherichia coli.


Assuntos
Anti-Infecciosos/farmacologia , Carvão Mineral/microbiologia , Isocumarinas/farmacologia , Penicillium/química , Anti-Infecciosos/química , Bactérias/efeitos dos fármacos , Espectroscopia de Ressonância Magnética Nuclear de Carbono-13 , China , Fungos/efeitos dos fármacos , Isocumarinas/química , Testes de Sensibilidade Microbiana , Espectroscopia de Prótons por Ressonância Magnética
4.
Zhongguo Zhong Yao Za Zhi ; 45(15): 3689-3693, 2020 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-32893559

RESUMO

Eleven compounds were isolated and purified from Sorghum vulgare root extract, through column chromatography over silica gel, MCI gel, and preparative HPLC. Their structures were established by MS, 1 D NMR and 2 D NMR data as sorgholide A(1), ß-sitosterol(2), stigmastero(3), daucosterol(4), 4-methoxycinnamic acid(5), taxiphyllin(6), chlorogenic acid(7), p-hydroxybenzaldehyde(8), succini acid(9), trans-p-hydroxycinnamic acid(10), obtusalin(11). Compounds 4,5 and 9-11 were reported from this species for the first time, and compound 1 is the first 24 ring dimeric double lactonol glycoside formed by reverse polymerization of p-hydroxyphenylacetate glucoside, named sorgholide A.


Assuntos
Glicosídeos Cardíacos , Sorghum , Glucosídeos , Glicosídeos , Fenóis
5.
Pharmacol Res ; 160: 105147, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32814167

RESUMO

EPHB6 is a metastasis inhibitory gene that is frequently decreased or deficiency in non-small cell lung cancer (NSCLC), which contributed to the subsequent development of distant metastasis. These suggested the possibility that reactivation of EPHB6 might prevent the metastasis of NSCLC. Nevertheless, EPHB6 expression might also promote cancer cell growth and inhibit cell apoptosis by activating Akt and ERK pathway, apart from inhibition of migration and invasion. In the present study, we developed a novel quinazolin-4(3H)-one analog (DFX24) as a potential PI3Kα inhibitor, which inhibited both cell proliferation and metastasis of NSCLC cell lines. Investigation to the molecular mechanisms revealed DFX24 inhibited the cell growth and metastasis via inhibition of PI3Kα and ERK activity, as well as the increase in EPHB6 expression. In addition, DFX24 also induced cell cycle arrest and tumor cell apoptosis by inhibiting PI3K/Akt pathway and activating mitochondria-dependent pathway, respectively. These findings suggested that DFX24 might be considered as a novel drug candidate and may provide a potential therapy for NSCLC.


Assuntos
Antineoplásicos/farmacologia , Derivados de Benzeno/farmacologia , Carcinoma Pulmonar de Células não Pequenas/prevenção & controle , Neoplasias Pulmonares/prevenção & controle , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Morfolinas/farmacologia , Metástase Neoplásica/prevenção & controle , Fosfatidilinositol 3-Quinases/efeitos dos fármacos , Inibidores de Proteínas Quinases/farmacologia , Piridinas/farmacologia , Quinazolinas/farmacologia , Receptores da Família Eph/efeitos dos fármacos , Receptores da Família Eph/metabolismo , Sulfonamidas/farmacologia , Apoptose/efeitos dos fármacos , Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , Movimento Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Proteína Oncogênica v-akt/efeitos dos fármacos , Ensaios Antitumorais Modelo de Xenoenxerto
6.
Molecules ; 23(9)2018 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-30205503

RESUMO

In this study, six coordination polymers (CPs), {[Ag2(L)(CF3SO3)]·CF3SO3·2H2O·DMF}n (1), {[Ag(L)]·SbF6·4DMF·H2O}n (2), {[Zn(L)0.5(I)2]·3.75H2O}n (3), {[Cd2(L)(I)4(H2O)(DMF)]·4H2O·3DMF}n (4), {[Hg2(L)(I)4]·H2O·4DMF}n (5) and {[Hg2(L)(Cl)4]·2H2O·3DMF}n (6), were obtained based on the designed X-shaped urea-based ligand. X-ray single crystal diffraction analysis revealed that complex 1 displayed a 3D (3,4)-connected {6·8²}{64·8²}-tcj net. Complex 2 featured a 2D 4-connected {4³·6³} sheet. Complexes 3 and 5 exhibited a 1D polymeric loop chain. Complex 4 displayed a 1D polymeric fishbone chain. Complex 6 showed a 2D 4-connected {44·6²}-sql sheet. Structural comparison revealed that not only the metal ions, but also the anions played crucial roles in the control of final structures.


Assuntos
Complexos de Coordenação/química , Polímeros/química , Piridonas/química , Ureia/química , Cádmio/química , Cristalografia por Raios X , Ligação de Hidrogênio , Ligantes , Estrutura Molecular , Difração de Pó , Espectroscopia de Infravermelho com Transformada de Fourier , Ureia/análogos & derivados , Zinco/química
7.
Bioorg Med Chem Lett ; 26(16): 3870-5, 2016 08 15.
Artigo em Inglês | MEDLINE | ID: mdl-27436581

RESUMO

Oleanolic acid (OA), one of the major pentacyclic triterpenes abundantly present in nature, is a promising compound with various biological activities, including anti-inflammatory, anti-ulcer, hepatoprotective, antidiabetic, fungicidal and antiparasitic properties. Therefore, a series of derivatives of 1α,2α-epoxy-3ß-hydroxyl oleanolic acid derivatives were designed and synthesized, and their antibacterial activities were investigated in vitro. Based on these results, the compounds with antibacterial activity were screened by RT-PCR to determine whether they can regulate the expression of genes related to metabolism, haemolysis, and ß-lactamase in vitro, and the structure-microbicidal activity relationship of each compound was analyzed. Our study shows that some of the modifications in the synthetic compounds, such as the introduction of an ortho-cyano-substituted benzyl group and a short chain alkyl ester at the 28-carboxyl, as well as the introduction of an acetyl group at the 3-hydroxyl group of ring A, could enhance antibacterial activity. This provides basic evidence for the optimization of 1α,2α-epoxy-3ß-hydroxyl oleanolic acid derivatives. The antibacterial mechanism of the active OA derivatives appears to involve the regulation of expression of metabolism-associated genes in Escherichia coli, haemolysis-associated genes in Bacillus subtilis, metabolism-related genes in Klebsiella pneumonia and ß-lactamase-associated genes in Acinetobacter baumannii. Some OA derivatives were bactericidal to three of the strains and appeared to regulate gene expression associated with metabolism, haemolysis, and ß-lactamase in vitro. These newly designed OA derivatives possess unique antibacterial activities and may be potentially useful for prophylactic or therapeutic intervention of bacterial infections.


Assuntos
Antibacterianos/química , Ácido Oleanólico/análogos & derivados , beta-Lactamases/metabolismo , Acinetobacter/efeitos dos fármacos , Acinetobacter/genética , Acinetobacter/metabolismo , Antibacterianos/síntese química , Antibacterianos/farmacologia , Bacillus subtilis/efeitos dos fármacos , Bacillus subtilis/genética , Bacillus subtilis/metabolismo , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Escherichia coli/efeitos dos fármacos , Escherichia coli/genética , Escherichia coli/metabolismo , Expressão Gênica/efeitos dos fármacos , Hemólise/efeitos dos fármacos , Klebsiella pneumoniae/efeitos dos fármacos , Klebsiella pneumoniae/genética , Klebsiella pneumoniae/metabolismo , Testes de Sensibilidade Microbiana , Ácido Oleanólico/síntese química , Ácido Oleanólico/farmacologia , Reação em Cadeia da Polimerase em Tempo Real , Relação Estrutura-Atividade , Triterpenos/química , beta-Lactamases/genética
8.
J Nat Prod ; 79(4): 721-31, 2016 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-26928299

RESUMO

The oleanane-type triterpene 18ß-glycyrrhetinic acid (1) was modified chemically through the introduction of a trihydroxylated A ring and an ester moiety at C-20 to enhance its antibacterial activity. Compounds 22, 23, 25, 28, 29, 31, and 32 showed more potent inhibitory activity against Streptomyces scabies than the positive control, streptomycin. Additionally, the inhibitory activity of the most potent compound, 29, against Bacillus subtilis, Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus was greater than that of the positive controls. The antibacterial mode of action of the active derivatives involved the regulation of the expression of genes associated with peptidoglycans, the respiratory metabolism, and the inherent virulence factors found in bacteria, as determined through a quantitative real-time reverse transcriptase PCR assay.


Assuntos
Antibacterianos/farmacologia , Ácido Glicirretínico/análogos & derivados , Bactérias Gram-Positivas/efeitos dos fármacos , Ampicilina/farmacologia , Antibacterianos/química , Bacillus subtilis/efeitos dos fármacos , Relação Dose-Resposta a Droga , Ácido Glicirretínico/química , Ácido Glicirretínico/farmacologia , Glycyrrhiza/química , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Estrutura Molecular , Pseudomonas aeruginosa/efeitos dos fármacos , Reação em Cadeia da Polimerase em Tempo Real , Streptomyces/efeitos dos fármacos , Estreptomicina/farmacologia , Relação Estrutura-Atividade , Vancomicina/farmacologia
9.
Zhongguo Zhong Yao Za Zhi ; 41(3): 451-455, 2016 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-28868863

RESUMO

Rosa roxburghii, a kind of the medical and edible plants belonging to the Rosaceae family, is widely distributed in the southwest districts of China, especially Guizhou province. Now, by reason of the extensive bioactivities, the plant is widely used in the field of food, health product, drug, and so on. In the course of our continuing search for the bioactive constituents, thirteen compounds were isolated from R. roxburghii, and their structures were determined on the basis of physicochemical property, spectroscopic data and comparison with the literatures, as 2-oxo pomolic acid(1), 1ß-hydroxyeuscaphic acid(2), euscaphic acid(3), arjunic acid(4), tormentic acid(5), kaiiichigeside F1(6), rosamultin(7), arjunetin(8), 2ɑ, 3ɑ, 19ɑ-trihydroxy-olean-12-en-28-oic acid 28-O-ß-D-glucopyranoside(9), 2α, 3α, 19α, 24-tetrahydroxyolean-12-en-28-oic-acid 28-O-ß-D-glucopyranosyl ester(10), pyrogallic acid (11), daucosterol(12), and 1, 2-decanediol(13). Compounds 9 and 10 were firstly obtained from Rosaceae family, and compounds 1,4,5,9-11,13 were isolated from this plant for the first time.


Assuntos
Extratos Vegetais/química , Rosa/química , China , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Extratos Vegetais/isolamento & purificação , Plantas Comestíveis/química , Espectrometria de Massas por Ionização por Electrospray
10.
J Pharm Pharmacol ; 67(1): 107-16, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25212982

RESUMO

OBJECTIVES: The aims of this study were to identify the active ingredients from Portulaca oleracea L. (PO) that could provide synergism with antibiotics against methicillin-resistant Staphylococcus aureus (MRSA) and their possible mechanisms of resistance inhibition. METHODS: High-speed counter-current chromatography (HSCCC) coupled with gas chromatography-mass spectrometry and a panel of laboratory MRSA strains were used for checkerboard and efflux inhibitory assays. KEY FINDINGS: Linoleic and oleic acids were identified from HSCCC fraction 18 of PO with synergistic antibacterial activity when combined with erythromycin against RN4220/pUL5054. Ethidium bromide efflux inhibitory studies revealed that linoleic and oleic acids may interfere the activity of MsrA pump. By comparing among a panel of linoleic and oleic acids analogues, unsaturated fatty acids in salt form with cis configuration and an increase in number of double bonds were found to further increase the antibacterial activity when used alone or in combination with antibiotics. CONCLUSION: This study reported for the first time that two active ingredients, namely linoleic and oleic acids, were identified from PO with synergistic antibacterial activity when combined with erythromycin against MRSA RN4220/pUL5054 and possibly act by inhibiting the efflux pumps of the bacteria cells.


Assuntos
Antibacterianos/farmacologia , Eritromicina/farmacologia , Ácido Linoleico/farmacologia , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Ácido Oleico/farmacologia , Portulaca , Ciprofloxacina/farmacologia , Sinergismo Farmacológico , Quimioterapia Combinada , Cromatografia Gasosa-Espectrometria de Massas , Ácido Linoleico/química , Testes de Sensibilidade Microbiana , Ácido Oleico/química , Extratos Vegetais/química
11.
Neurol Res ; 37(2): 184-8, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25034027

RESUMO

Although neurovascular confliction was believed to be the cause of hemifacial spasm (HFS), the mechanism of the disorder remains unclear to date. Current theories, merely focusing on the facial nerve, have failed to explain the clinical phenomenon of immediate relief following a successful microvascular decompression surgery (MVD). With the experience of thousands of microvascular decompression surgeries and preliminary investigations, we have learned that the offending artery may play a more important role than the effect of merely mechanical compression in the pathogenesis of the disease. We believe that the attrition of neurovascular interface is the essence of the etiology, and the substance of the disease is emersion of ectopic action potentials from the demyelinated facial nerve fibers, which were triggered by the sympathetic endings from the offending artery wall. In this paper, we put forward evidence to support this hypothesis, both logically and theoretically.


Assuntos
Potenciais de Ação/fisiologia , Doenças Desmielinizantes/fisiopatologia , Nervo Facial/fisiopatologia , Espasmo Hemifacial/etiologia , Espasmo Hemifacial/fisiopatologia , Nervo Facial/ultraestrutura , Espasmo Hemifacial/cirurgia , Humanos , Cirurgia de Descompressão Microvascular
12.
J Craniofac Surg ; 24(3): e286-9, 2013 May.
Artigo em Inglês | MEDLINE | ID: mdl-23714994

RESUMO

Painful tic convulsif is referred to as the concurrent trigeminal neuralgia and hemifacial spasm. However, painful tic convulsif after ipsilateral Bell palsy has never been reported before. We report a case of a 77-year-old woman with coexistent trigeminal neuralgia and hemifacial spasm who had experienced Bell palsy half a year ago. The patient underwent microvascular decompression. Intraoperatively, the vertebrobasilar artery was found to deviate to the symptomatic side and a severe adhesion was observed in the cerebellopontine angle. Meanwhile, an ectatic anterior inferior cerebellar artery and 2 branches of the superior cerebellar artery were identified to compress the caudal root entry zone (REZ) of the VII nerve and the rostroventral cisternal portion of the V nerve, respectively. Postoperatively, the symptoms of spasm ceased immediately and the pain disappeared within 3 months. In this article, the pathogenesis of the patient's illness was discussed and it was assumed that the adhesions developed from inflammatory reactions after Bell palsy and the anatomic features of the patient were the factors that generated the disorder. Microvascular decompression surgery is the suggested treatment of the disease, and the dissection should be started from the caudal cranial nerves while performing the operation.


Assuntos
Paralisia de Bell/complicações , Descompressão Cirúrgica/métodos , Espasmo Hemifacial/cirurgia , Microcirurgia/métodos , Neuralgia do Trigêmeo/cirurgia , Idoso , Artéria Basilar/anormalidades , Artéria Basilar/cirurgia , Doenças Cerebelares/etiologia , Doenças Cerebelares/cirurgia , Ângulo Cerebelopontino/patologia , Cerebelo/irrigação sanguínea , Doenças do Nervo Facial/etiologia , Doenças do Nervo Facial/cirurgia , Feminino , Seguimentos , Espasmo Hemifacial/etiologia , Humanos , Síndromes de Compressão Nervosa/etiologia , Síndromes de Compressão Nervosa/cirurgia , Telangiectasia/complicações , Aderências Teciduais/etiologia , Aderências Teciduais/cirurgia , Doenças do Nervo Trigêmeo/etiologia , Doenças do Nervo Trigêmeo/cirurgia , Neuralgia do Trigêmeo/etiologia , Artéria Vertebral/anormalidades , Artéria Vertebral/cirurgia , Doenças do Nervo Vestibulococlear/etiologia , Doenças do Nervo Vestibulococlear/cirurgia
13.
J Asian Nat Prod Res ; 15(3): 270-5, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23421779

RESUMO

Two new coumarin glycosides, namely nitensosides A-B (1-2), together with six known compounds, scopolin (3), 5,6,7-trimethoxycoumarin (4), d-calycanthine (5), calycanthoside (6), xeroboside (7), and scopoletin (8), were isolated from Chimonanthus nitens. The structures of the new compounds were elucidated by comprehensive analysis of IR, MS, and NMR spectroscopic data. Compounds 3, 4, 7, and 8 showed moderate inhibitory activity against Micrococcus luteus.


Assuntos
Calycanthaceae/química , Cumarínicos/isolamento & purificação , Flavonas/isolamento & purificação , Glucosídeos/isolamento & purificação , Glicosídeos/isolamento & purificação , Cumarínicos/química , Cumarínicos/farmacologia , Flavonas/química , Flavonas/farmacologia , Glucosídeos/química , Glucosídeos/farmacologia , Glicosídeos/química , Glicosídeos/farmacologia , Testes de Sensibilidade Microbiana , Micrococcus luteus/efeitos dos fármacos , Estrutura Molecular , Naftiridinas/isolamento & purificação , Ressonância Magnética Nuclear Biomolecular
14.
J Craniofac Surg ; 23(5): 1385-7, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22948645

RESUMO

OBJECTIVE: Although microvascular decompression (MVD) has been accepted as effective therapy for hemifacial spasm, failed surgery has been reported frequently. For a sophisticated neurosurgeon, an apparent offending artery is seldom missed. However, it is still an embarrassed situation when the neurovascular conflict site could not be approached. METHODS: Clinical data were collected from consecutive 211 MVDs in 2010. Intraoperative abnormal muscle response was recorded. Among them, the neurovascular conflict was not finally discovered in 3 patients, whom were then focused on. All patients were followed up for 6 to 15 months. RESULTS: In 17 of the 211 MVDs, the cerebellum was hard to be retracted because of adhesions. After careful dissection, a working space was finally created in the cerebellopontine angle. However, there still were 3 cases, whose neurovascular conflict site was unable to be discovered at last because of a branch of an artery embedded in the petrous bone and made the cerebellum unmovable. With navigation of real-time abnormal muscle response, the offending artery was moved away eventually even without exposing the conflict site. Postoperatively, all the patients were completely spasm-free immediately. No recurrence was noticed in the last follow-up period. CONCLUSIONS: The most important thing for a successful MVD operation is to remove the offending artery off the nerve. However, if the conflict site failed to be approached after endeavors, a successful MVD can still be achieved by relocating the offending artery with the guidance of real-time electromyography even without visualization of the confliction.


Assuntos
Espasmo Hemifacial/cirurgia , Cirurgia de Descompressão Microvascular/métodos , Ângulo Cerebelopontino/cirurgia , Eletromiografia , Músculos Faciais/irrigação sanguínea , Músculos Faciais/cirurgia , Feminino , Espasmo Hemifacial/fisiopatologia , Humanos , Masculino , Pessoa de Meia-Idade , Monitorização Intraoperatória , Resultado do Tratamento
15.
Zhongguo Zhen Jiu ; 32(4): 351-3, 2012 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-22734390

RESUMO

OBJECTIVE: To observe the efficacy differences of acupoints massage for asthenopia of video display terminal (VDT) under different exposure dose. METHODS: One hundred and two cases (204 eyes) were divided into a low exposure group and a high exposure group, fifty-one cases in each group. The same intervention of acupoints massage on Cuanzhu (BL 2), Jingming (BL 1), Sizhukong (TE 23), Sibai (ST 2) and Taiyang(EX-HN 5) were given to the two groups, one acupoint for 5 min and once everyday, one month of which made a course. The symptom score, tear film break-up time (BUT) and Schirmer I test(SIT) were observed before and after treatment. RESULTS: (1) The correlation coefficient of cubic curve model of the exposure dose was the biggest with symptom improvement index (P = 0.000), which indicated that the lower VDT exposure index was, the more obvious the symptom improved. The symptom improvement indices of low exposure group and high exposure group, which were (52.31 +/- 16.65)% and (28.93 +/- 13.35)% respectively, were statistical significant difference (P = 0.000). (2) Compared to before treatment, the levels of BUT and SIT in the two groups were both significantly higher (P < 0.05). Compared with the high exposure group, the levels of BUT and SIT in the low exposure group were increased by 0.826 s (P = 0.022) and 1.029 mm (P = 0.033), respectively, after the impact of BUT and SIT was corrected before the research. CONCLUSION: The acupoints massage can improve the symptoms and ocular physiology for patients with VDT asthenpia, and it is more effective for the low exposure cases.


Assuntos
Pontos de Acupuntura , Astenopia/terapia , Massagem , Doenças Profissionais/terapia , Adulto , Astenopia/fisiopatologia , Terminais de Computador/estatística & dados numéricos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Doenças Profissionais/fisiopatologia , Lágrimas/metabolismo , Resultado do Tratamento , Adulto Jovem
16.
Neurol Res ; 34(7): 643-8, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22663936

RESUMO

OBJECTIVES: Despite the vascular compression of the seventh cranial nerve has been verified by the microvascular decompression surgery as the cause of hemifacial spasm (HFS), the mechanism of the disease is still unknown. We believe that the autonomic nervous system in adventitia of the offending artery may contribute to the HFS. To prove our hypothesis, we performed an experiment in SD rats. METHODS: Moller's HFS model was adopted and the abnormal muscle response (AMR) wave was electrophysiologically monitored. With randomization, some HFS rats underwent exclusion of the offending artery or removal of the ipsilateral superior cervical ganglion. Some HFS rats with negative AMR following exclusion of the offending artery were dripped with norepinephrine onto the neurovascular conflict site. RESULTS: With exclusion of the offending artery, AMR disappeared in 14 (70%) of the 20 HFS rats, while in three (30%) of the 10 from sham operation group (P<0·05). With ganglionectomy, AMR disappeared in 12 (75%) of the 16 HFS rats, while in two (25%) of the eight from the sham operation group (P<0·05). With norepinephrine drip, AMR reappeared in four (67%) of the six from those offending-artery-excluded HFS rats, while in zero of the six from normal-saline-dripped group (P<0·05). DISCUSSION: The neurotransmitter releasing from the autonomic nervous endings in the worn adventitia of the offending artery may induce an ectopia action potential in those demyelinated facial nerve fibers expanding to the neuromuscular conjunction and trigger an attack of HFS.


Assuntos
Sistema Nervoso Autônomo/fisiologia , Espasmo Hemifacial/fisiopatologia , Animais , Sistema Nervoso Autônomo/irrigação sanguínea , Músculos Faciais/irrigação sanguínea , Músculos Faciais/inervação , Espasmo Hemifacial/etiologia , Masculino , Ratos , Ratos Sprague-Dawley , Artérias Temporais/fisiologia
17.
Acta Neurochir (Wien) ; 154(5): 793-7; discussion 797, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-22441581

RESUMO

BACKGROUND: Due to its anatomical features, the vertebrobasilar artery complex (VBA) seldom contributes to the neurovascular conflict in patient with trigeminal neuralgia (TN). However, once it offends the trigeminal root, this large artery is really difficult to manipulate during microvascular decompression (MVD) surgery. Therefore, the surgical strategy for such cases needs to be detailed in order to obtain a satisfactory outcome. METHODS: From 2009 through 2011, 475 consecutive TN patients underwent MVDs in our department. Among them, ten were found in which an ipsilateral deviating ectatic vertebrobasilar artery complex (VBA) offended the trigeminal nerve. Those cases were focused on in this study and each operation was analyzed retrospectively. RESULTS: During the operation, the vertebral artery was regarded as the direct culprit in six (60 %) patients, while the basilar artery in four (40 %). As companions, some smaller vessels were also observed to be close to the nerve, including the superior cerebellar artery (SCA) in five, veins in two and anterior inferior cerebellar artery (AICA) in two. The neurovascular conflict was discovered in the cisternal segment of the trigeminal root in eight, while in the root entry zone (REZ) in two. In six out of the ten cases, the affected nerves were demonstrated to be squeezed towards the tentorium by the ectatic VBA. Postoperatively, the symptom of pain totally disappeared immediately in eight (80 %) patients, while it was relieved apparently in two (20 %). During the follow-up period of 3-30 months, no recurrence or complication was found, except for one patient who had numbness of the face. CONCLUSION: With a proper strategy, MVD is probably the most effective therapy for the TN cases caused by ectatic vertebrobasilar artery complex. The substance of the surgery is to withdraw the proximal vertebral artery caudally via a lateroinferior cerebellar approach.


Assuntos
Cirurgia de Descompressão Microvascular/métodos , Neuralgia do Trigêmeo/cirurgia , Idoso , Idoso de 80 Anos ou mais , Artéria Basilar/cirurgia , Feminino , Seguimentos , Humanos , Masculino , Cirurgia de Descompressão Microvascular/efeitos adversos , Pessoa de Meia-Idade , Resultado do Tratamento , Neuralgia do Trigêmeo/etiologia , Artéria Vertebral/cirurgia
18.
Clin Neurol Neurosurg ; 114(7): 846-51, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22310997

RESUMO

OBJECTIVE: Despite the microvascular decompression (MVD) has become a definitive treatment for trigeminal neuralgia (TN) and hemifacial spasm (HFS), not all of the patients have been cured completely so far and this sort of operation is still with risk because of the critical operative area. In order to refine this surgery, we investigated thousands MVDs. METHODS: Among 3000 consecutive cases of MVDs have been performed in our department, 2601 were those with typical TN or HFS, who were then enrolled in this investigation. They were retrospectively analyzed with emphasis on the correlation between surgical findings and postoperative outcomes. The differences between TN and HFS cases were compared. The strategy of each surgical process of MVD was addressed. RESULTS: Postoperatively, the pain free or spasm cease occurred immediately in 88.3%. The symptoms improved at some degree in 7.2%. The symptoms unimproved at all in 4.5%. Most of those with poor outcome underwent a redo MVD in the following days. Eventually, their symptoms were then improved in 98.7% of the reoperative patients. The majority reason of the failed surgery was that the neurovascular conflict located beyond REZ or the offending veins were missed for TN, while the exact offending artery (arteriole) was missed for HFS as it located far more medially than expected. CONCLUSION: A prompt recognition of the conflict site leads to a successful MVD. To facilitate the approach, the craniotomy should be lateral enough to the sigmoid sinus. The whole intracranial nerve root should be examined and veins or arterioles should not be ignored. For TN, all the vessels contacting the nerve should be detached. For HFS, the exposure should be medial enough to the pontomedullary sulcus.


Assuntos
Espasmo Hemifacial/cirurgia , Cirurgia de Descompressão Microvascular/métodos , Neuralgia do Trigêmeo/cirurgia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Arteríolas/cirurgia , Criança , Craniotomia , Dura-Máter/cirurgia , Nervo Facial/cirurgia , Feminino , Espasmo Hemifacial/diagnóstico , Humanos , Masculino , Pessoa de Meia-Idade , Monitorização Intraoperatória , Reoperação , Estudos Retrospectivos , Falha de Tratamento , Resultado do Tratamento , Neuralgia do Trigêmeo/diagnóstico , Veias/cirurgia , Adulto Jovem
19.
Zhongguo Zhong Xi Yi Jie He Za Zhi ; 31(8): 1108-12, 1117, 2011 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-21910346

RESUMO

OBJECTIVE: To investigate anticancer effects of 5-fluorouracil (5-FU) combined with CL, extract of Rosa roxburghii Tratt on human endometrial adenocarcinoma cell line (JEC). METHODS: JEC cells cultured in vitro in the logarithmic growth phase were seeded in the culture plate and divided into the control group (RPMI 1640), the positive group (10(-4) mol/L 5-FU), the CL groups (at the dose of 0.01, 0.1, 1, 10, and 100 microg/mL), and the CL (0.01, 0.1, 1, 10, and 100 microg/mL) combined with 5-FU groups. Effects of 5-FU combined with CL on JEC cell growth were drawn and measured by MTT and growth curves. Effects of CL combined with 5-FU on the JEC cell differentiation was analyzed by detecting the reduction capability of nitrobenzene thiocyanate (NBT) and lactate dehydrogenase (LDH) contents in the cultured medium. Effects of CL combined with 5-FU on the JEC cell apoptosis and cell proliferation cycle were detected by acridine orange (AO)/ethidium bromide (EB) fluorescent staining and flow cytometry (FCM). RESULTS: The proliferation inhibitory effect of CL combined with 5-FU on JEC cells was enhanced when compared with that of CL or 5-FU alone (P<0.05). The percentages of NBT positive JEC cells and apoptotic JEC cells increased in the 5-FU combined with CL groups when compared with 5-FU group or the CL group alone (P<0.05). The LDH concentration of the JEC cell culture supernate decreased in 5-FU combined with CL groups (P<0.05). Furthermore, the percentage of G0-G1 phase JEC cells treated by 5-FU combined with CL was higher than that of 5-FU or CL alone (P<0.05). CONCLUSION: CL could enhance anticancer effects of 5-FU. Its mechanisms might be correlated with reinforcing the cytotoxicity of 5-FU, inducing cell differentiation and apoptosis, and inhibiting cell proliferation and division.


Assuntos
Diferenciação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Fluoruracila/farmacologia , Extratos Vegetais/farmacologia , Adenocarcinoma/patologia , Linhagem Celular Tumoral , Neoplasias do Endométrio/patologia , Feminino , Interações Ervas-Drogas , Humanos , Rosa/química
20.
Zhong Yao Cai ; 34(2): 223-5, 2011 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-21823478

RESUMO

OBJECTIVE: To study the chemical constituents of Laportea bulbifera. METHODS: 70% EtOH was used for constituent extraction, silica gel column chromatography for constituent seperation, physical and chemical properties together with spectroscopic methods for chemical structure identification. RESULTS: six compounds were obtained from root of L. bulbifera. Their chemical structures were elucidated as p3-sitosterol(1) , P-daucosterol (2), 2,2'-oxy-bis(1-phenylethanol (3), 1-(2-phenylcarbonyloxy acetyl) benzene (4) , methyl linoleate(5),1,4-diphenyl-1,4-butanedione(6). CONCLUSION: All compounds are isolated from L. bulbifera and among compounds 3 and 4 are reported as natural products for the first time.


Assuntos
Extratos Vegetais/isolamento & purificação , Plantas Medicinais/química , Urticaceae/química , Ácidos Linoleicos/química , Ácidos Linoleicos/isolamento & purificação , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Extratos Vegetais/química , Rizoma/química , Sitosteroides/química , Sitosteroides/isolamento & purificação
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