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1.
J Phys Condens Matter ; 32(2): 025704, 2019 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-31536973

RESUMO

Herein, we investigated the pressure dependence of electric transport in a new type of superconducting metal iridate compound, SrIr2, that exhibits a superconducting transition temperature, T c, as high as 6.6 K at ambient pressure, in order to complete the T c-pressure (p ) phase diagram. Very recently, this sample's superconductivity was discovered by our group, but the superconducting behavior has not yet been clarified under pressure. In this study, we fully investigated this sample's superconductivity in a wide pressure range. The T c value decreased with an increase in pressure, but the onset superconducting transition temperature, [Formula: see text], increased above a pressure of 8 GPa, indicating an unconventional superconductivity different from a BCS-type superconductor. The magnetic field dependence of electric resistance (R) against temperature (R - T plot) recorded at 7.94 and 11.3 GPa suggested an unconventional superconductivity, followed by a p -wave polar model, supporting the deviation from a simple s-wave pairing. Moreover, we fully investigated the pressure dependence of crystal structure in SrIr2 and discussed the correlation between superconductivity and crystal structure. This is the first systematic study on superconducting behavior of a new type of metal iridate compound, MIr2 (M: alkali-earth metal atom), under pressure.

2.
Cell Commun Signal ; 17(1): 117, 2019 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-31500648

RESUMO

BACKGROUND: Cryptococcus neoformans (C. neoformans) is an encapsulated budding yeast that causes life-threatening meningoencephalitis in immunocompromised individuals, especially those with acquired immunodeficiency syndrome (AIDS). To cause meningoencephalitis, C. neoformans circulating in the bloodstream must first be arrested in the brain microvasculature. Neutrophils, the most abundant phagocytes in the bloodstream and the first leukocytes to be recruited to an infection site, can ingest C. neoformans. Little is known about how neutrophils interact with arrested fungal cells in the brain microvasculature. METHODS: A blood-brain barrier (BBB) in vitro model was established. The interactions between neutrophils adhering to brain endothelial cells and fungi were observed under a live cell imaging microscope. A flow cytometry assay was developed to explore the mechanisms. Immunofluorescence staining of brain tissues was utilized to validate the in vitro phenomena. RESULTS: Using real-time imaging, we observed that neutrophils adhered to a monolayer of mouse brain endothelial cells could expel ingested C. neoformans without lysis of the neutrophils or fungi in vitro, demonstrating nonlytic exocytosis of fungal cells from neutrophils. Furthermore, nonlytic exocytosis of C. neoformans from neutrophils was influenced by either the fungus (capsule and viability) or the neutrophil (phagosomal pH and actin polymerization). Moreover, nonlytic exocytosis of C. neoformans from neutrophils was recorded in brain tissue. CONCLUSION: These results highlight a novel function by which neutrophils extrude C. neoformans in the brain vasculature.

3.
Artigo em Inglês | MEDLINE | ID: mdl-31091739

RESUMO

Batch experiments were completed to assess the sludge reduction of the metabolic uncoupler 3,3',4',5-tetrachlorosalicylanilide (TCS). The effects of various TCS concentrations on sludge yield were evaluated and the mechanisms associated with sludge reduction were assessed. We discovered that TCS addition resulted in a reduction in sludge. Furthermore, a low dose of TCS (≤3 mg/L) resulted in a slight reduction in the efficiency of the wastewater treatment system, while >3 mg/L TCS reduced matrix removal efficiency, with an especially remarkable inhibition effect on ammonia removal. An increase in TCS addition was associated with a gradual decrease in both the electron transport system (ETS) activity and the specific cellular ATP (SATP) in the TCS system. It was demonstrated that TCS plays an important role in metabolic uncoupling. However, with the addition of TCS, both contents and compositions were increased, and the protein content increased more than polysaccharide production in extracellular polymeric substances (EPS). At TCS concentrations of ≤3 mg/L, DNA content was stable, but it increased rapidly from 4.97 mg/L to 15.34 mg/L as the TCS concentration was elevated from 6 mg/L to 12 mg/L. This implied that the mechanisms of sludge reduction were different for different TCS concentrations, including uncoupling metabolism, maintenance metabolism and lysis-cryptic growth.

4.
Artigo em Inglês | MEDLINE | ID: mdl-31075982

RESUMO

Ofloxacin, sulfamethoxazole and ibuprofen are three commonly used drugs which can be detected in aquatic environments. To assess their ecotoxicity, the effects of these three pharmaceuticals and their mixture on AChE (acetylcholinesterase) activity in the brain, and EROD (7-ethoxyresorufin-O-deethylase) and SOD (superoxide dismutase) activities in the liver of the freshwater crucian carp Carassius auratus were tested after exposure for 1, 2, 4 and 7 days. The results showed that treatments with 0.002⁻0.01 mg/L ofloxacin and 0.0008⁻0.004 mg/L sulfamethoxazole did not significantly change AChE, EROD and SOD activities. AChE activity was significantly inhibited in response to treatment with 0.05mg/L ofloxacin and 0.02mg/L sulfamethoxazole. All three biomarkers were induced significantly in treatments with ibuprofen and the mixture of the three pharmaceuticals at all the tested concentrations. The combined effects of ofloxacin, sulfamethoxazole and ibuprofen were compared with their isolated effects on the three biomarkers, and the results indicated that exposure to ibuprofen and the mixture at environmentally relevant concentrations could trigger adverse impacts on Carassius auratus. The hazard quotient (HQ) index also demonstrated a high risk for ibuprofen. Moreover, the present study showed that the effects of ofloxacin, sulfamethoxazole and ibuprofen might be additive on the physiological indices of Carassius auratus.

5.
PLoS One ; 14(5): e0210379, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31071092

RESUMO

The chive gnat, Bradysia odoriphaga, is a notorious pest of Allium species in China. Colour trapping is an established method for monitoring and control of Bradysia species. In order to clarify the effect of colour preference of B. odoriphaga for the perched substrate, multiple-choice tests were used to assess the response of the chive gnat to different colour hues and brightness levels under different intensities of white illumination and two spectrally different illuminations. Given the choice among four colours differing in hue under different intensities of white illumination and two spectrally different illuminations, chive gnat adults significant preferred the black substrate, a lesser preference to brown and green substrates, and the least preference to orange substrate irrespective of illumination. Given the choice among four levels of brightness under the same illumination conditions as those in the previous experiment (different intensities of white illumination and two spectrally different illuminations), chive gnats preferred black substrate over dark grey, light grey and white substrates. Meanwhile, both virgin and mated adults significantly preferred black over other colour hues and brightness. Based on our results, we conclude that the chive gnat adults significantly prefer black substrates irrespective of colour hues and brightness. This behaviour does not alter with ambient light condition changes. No difference observed between choices of female and male adults. Our results provide new insight for understanding the colour choice behaviour in chive gnat and pave a way to improve monitoring and control of chive gnats and management.

6.
Artigo em Inglês | MEDLINE | ID: mdl-30875848

RESUMO

KCl-extractable sediment dissolved organic nitrogen (KS-DON) extracted from sediments near drinking water intakes of six drinking water sources in Taihu Lake in China was partitioned into hydrophobic and hydrophilic fractions and high/low molecular weight fractions. The results showed that the total dissolved nitrogen (TDN) contents of the extracts ranged from 67.78 to 128.27 mg/kg. KS-DON was the main TDN species, accounting for more than 50%, with NH4⁺-N and NO3--N averaging 30% and 20%, respectively. The molecular weight fractions of <1 kDa accounted for almost half of KS-DON. Hydrophilic compounds accounted for more than 75% of KS-DON. Three fluorescence peaks were identified: soluble microbial byproducts (A); protein-like substances (B); and humic acid-like substances (C). It is concluded that the KS-DON in Taihu Lake sources has higher bioavailability and higher risk of endogenous release. Ecological dredging and establishment of constructed wetlands are possible measures to reduce the release of endogenous nitrogen.


Assuntos
Sedimentos Geológicos/química , Lagos/química , Nitrogênio/análise , Compostos Orgânicos/análise , Poluentes Químicos da Água/análise , China , Monitoramento Ambiental , Recursos Hídricos
7.
Sensors (Basel) ; 19(2)2019 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-30634722

RESUMO

Wireless sensor networks (WSNs) are vulnerable to computer viruses. To protect WSNs from virus attack, the virus library associated with each sensor node must be updated in a timely way. This article is devoted to developing energy-efficient patching strategies for WSNs. First, we model the original problem as an optimal control problem in which (a) each control stands for a patching strategy, and (b) the objective functional to be optimized stands for the energy efficiency of a patching strategy. Second, we prove that the optimal control problem is solvable. Next, we derive the optimality system for solving the optimal control problem, accompanied with a few examples. Finally, we examine the effects of some factors on the optimal control. The obtained results help improve the security of WSNs.

8.
Cell Commun Signal ; 16(1): 97, 2018 12 07.
Artigo em Inglês | MEDLINE | ID: mdl-30526602

RESUMO

BACKGROUND: Transforming growth factor beta 1 (TGF-ß1) is a classical modulator of skeletal muscle and regulates several processes, such as myogenesis, regeneration and muscle function in skeletal muscle diseases. Skeletal muscle atrophy, characterized by the loss of muscle strength and mass, is one of the pathological conditions regulated by TGF-ß1, but the underlying mechanism involved in the atrophic effects of TGF-ß1 is not fully understood. METHODS: Mice sciatic nerve transection model was created and gastrocnemius were analysed by western blot, immunofluorescence staining and fibre diameter quantification after 2 weeks. Exogenous TGF-ß1 was administrated and high-mobility group box-1 (HMGB1), autophagy were blocked by siRNA and chloroquine (CQ) respectively to explore the mechanism of the atrophic effect of TGF-ß1 in denervated muscle. Similar methods were performed in C2C12 cells. RESULTS: We found that TGF-ß1 was induced in denervated muscle and it could promote atrophy of skeletal muscle both in vivo and in vitro, up-regulated HMGB1 and increased autophagy activity were also detected in denervated muscle and were further promoted by exogenous TGF-ß1. The atrophic effect of TGF-ß1 could be inhibited when HMGB1/autophagy pathway was blocked. CONCLUSIONS: Thus, our data revealed that TGF-ß1 is a vital regulatory factor in denervated skeletal muscle in which HMGB1/ autophagy pathway mediates the atrophic effect of TGF-ß1. Our findings confirmed a new pathway in denervation-induced skeletal muscle atrophy and it may be a novel therapeutic target for patients with muscle atrophy after peripheral nerve injury.


Assuntos
Autofagia , Denervação , Proteína HMGB1/metabolismo , Músculo Esquelético/patologia , Atrofia Muscular/metabolismo , Atrofia Muscular/patologia , Fator de Crescimento Transformador beta1/metabolismo , Animais , Linhagem Celular , Regulação da Expressão Gênica , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Músculo Esquelético/inervação , Músculo Esquelético/metabolismo
9.
PLoS One ; 13(12): e0208738, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30592727

RESUMO

Viral marketing has been one of the main marketing modes. However, theoretical study of viral marketing is still lacking. This paper focuses on the problem of developing a cost-effective dynamic discount pricing strategy for a viral marketing campaign. First, based on a novel word-of-mouth propagation model, we model the original problem as an optimal control problem. Second, we show that the optimal control problem admits an optimal control and present the optimality system for solving the optimal control problem. Next, we solve some optimal control models to get their respective optimal dynamic discount pricing strategies. Finally, we examine the effect of some factors on the maximum marketing profit. These results contribute to gaining insight into viral marketing.


Assuntos
Custos e Análise de Custo , Marketing , Modelos Econômicos , Simulação por Computador , Humanos
10.
Environ Pollut ; 243(Pt B): 1710-1718, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30408858

RESUMO

The estimation of PM2.5-related mortality is becoming increasingly important. The accuracy of results is largely dependent on the selection of methods for PM2.5 exposure assessment and Concentration-Response (C-R) function. In this study, PM2.5 observed data from the China National Environmental Monitoring Center, satellite-derived estimation, widely collected geographic and socioeconomic information variables were applied to develop a national satellite-based Land Use Regression model and evaluate PM2.5 exposure concentrations within 2013-2015 with the resolution of 1 km × 1 km. Population weighted concentration declined from 72.52 µg/m3 in 2013 to 57.18 µg/m3 in 2015. C-R function is another important section of health effect assessment, but most previous studies used the Integrated Exposure Regression (IER) function which may currently underestimate the excess relative risk of exceeding the exposure range in China. A new Shape Constrained Health Impact Function (SCHIF) method, which was developed from a national cohort of 189,793 Chinese men, was adopted to estimate the PM2.5-related premature deaths in China. Results showed that 2.19 million (2013), 1.94 million (2014), 1.65 million (2015) premature deaths were attributed to PM2.5 long-term exposure, different from previous understanding around 1.1-1.7 million. The top three provinces of the highest premature deaths were Henan, Shandong, Sichuan, while the least ones were Tibet, Hainan, Qinghai. The proportions of premature deaths caused by specific diseases were 53.2% for stroke, 20.5% for ischemic heart disease, 16.8% for chronic obstructive pulmonary disease and 9.5% for lung cancer. IER function was also used to calculate PM2.5-related premature deaths with the same exposed level used in SCHIF method, and the comparison of results indicated that IER had made a much lower estimation with less annual amounts around 0.15-0.5 million premature deaths within 2013-2015.

11.
Nanomaterials (Basel) ; 8(10)2018 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-30322199

RESUMO

We propose and numerically demonstrate two novel terahertz absorbers made up of periodic single- and double-layer decussate graphene ribbon arrays. The simulated results show that the proposed absorbers have narrowband near-unity terahertz absorption with ultra-wide frequency reconfiguration and angular stability. By tuning the Fermi level of graphene ribbons, the over 90% absorbance peak frequency of the absorber with single-layer graphene structure can be flexibly adjusted from 6.85 to 9.85 THz for both the transverse magnetic (TM) and transverse electric (TE) polarizations. This absorber with single-layer graphene demonstrates excellent angular stability with the absorbance peaks of the reconfigurable absorption bands remaining over 99.8% in a wide angle of incidence ranging from 0 to 70°. The tuning frequency can be significantly enhanced by using the absorber with double-layer graphene structure from 5.50 to 11.28 THz and 5.62 to 10.65 THz, approaching two octaves under TM and TE polarizations, respectively. The absorbance peaks of the reconfigurable absorption band of this absorber for both polarizations maintain over 70%, even at a large angle of incidence up to 70°. Furthermore, an analytical fitting model is also proposed to accurately predict the absorbance peak frequencies for this variety of absorbers. Benefitting from these attractive properties, the proposed absorber may have great potential applications in tunable terahertz trapping, detecting, sensing, and various terahertz optoelectronic devices.

12.
Oncol Rep ; 40(4): 2251-2259, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30066949

RESUMO

MicroRNAs (miRs) serve an essential role in tumorigenesis and are able to act as tumor suppressor genes or oncogenes. miR­106a has been identified generally as an oncogene in multiple types of human cancer; however, its association with osteosarcoma has not previously been understood. Reverse transcription­quantitative polymerase chain reaction (RT­qPCR) was used to detect miR­106a expression in 18 osteosarcoma tissues compared with paired non­cancerous adjacent tissues as well as osteosarcoma cell lines (U2OS, Saos­2 and MG63) compared with a normal osteoblast cell line (hFOB1.19). The biological function of U2OS cells was assessed by using a Transwell cell invasion assay, MTS proliferation assay and flow cytometric analysis following the transfection with lentivirus­mediated small interfering RNA (miR­106a­inhibitor). Western blotting and Luciferase reporters were used to investigate whether VNN2 was a target of miR­106a in osteosarcoma cells. Based on the RT­qPCR data, miR­106a was significantly upregulated in osteosarcoma tissues and osteosarcoma cell lines compared with their control counterparts (P<0.01). The knockdown of miR­106a resulted in cell proliferation and invasion inhibition. Furthermore, apoptosis enhancement and G2/M cell cycle arrest were detected by flow cytometry. The western blot analysis indicated that U2OS cells infected with miR­106a­inhibitor lentivirus had a higher VNN2 protein expression level compared with cells infected with miR­106a­negative control lentivirus. Luciferase reporters containing the 3'­untranslated region sequence of VNN2 messenger RNA demonstrated VNN2 may be a target of miR­106a. In addition, a negative correlation was confirmed between the expression of VNN2 and miR­106a in the tumor samples. The results of the present study indicate that the knockdown of miR­106a overexpressed VNN2 to inhibiting the proliferation, migration and invasion as well as inducing the apoptosis of human osteosarcoma cells.


Assuntos
Amidoidrolases/metabolismo , Biomarcadores Tumorais/metabolismo , Neoplasias Ósseas/patologia , Moléculas de Adesão Celular/metabolismo , Movimento Celular , Proliferação de Células , MicroRNAs/genética , Osteossarcoma/patologia , Adolescente , Adulto , Amidoidrolases/genética , Apoptose , Biomarcadores Tumorais/genética , Neoplasias Ósseas/genética , Neoplasias Ósseas/metabolismo , Estudos de Casos e Controles , Moléculas de Adesão Celular/genética , Ciclo Celular , Feminino , Seguimentos , Proteínas Ligadas por GPI/genética , Proteínas Ligadas por GPI/metabolismo , Regulação Neoplásica da Expressão Gênica , Humanos , Masculino , Invasividade Neoplásica , Osteoblastos/metabolismo , Osteoblastos/patologia , Osteossarcoma/genética , Osteossarcoma/metabolismo , Prognóstico , Células Tumorais Cultivadas , Adulto Jovem
13.
Folia Microbiol (Praha) ; 63(6): 669-676, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-29728998

RESUMO

Salmonella enteritidis infection occurs in enterogenous diseases, such as gastroenteritis and parenteral focal infection, which often involve inflammation of intestinal epithelial cells. The nuclear factor kappa B (NF-κB) pathway participates in the innate immune response to many gram-negative pathogenic bacteria and initiates inflammation in epithelial cells. KH-type splicing regulatory protein (KSRP) is a multi-domain RNA-binding protein that recruits the exosome-containing mRNA degradation complex to mRNAs coding for inflammatory response factors. However, it remains unclear whether KSRP is regulated by NF-κB signaling pathway in response to S. enteritidis infection and affects the development of inflammation. Accordingly, in this study, we investigated the role of KSRP in mediating the response to S. enteritidis in Caco-2 cells. The data revealed that S. enteritidis infection decreased KSRP expression, which was suppressed by blocking the NF-κB pathway. Additionally, S. enteritidis infection significantly increased the expression of inducible nitric oxide synthase and cyclooxygenase-2. Overexpression of KSRP reduced the expression levels of inflammatory factors in Caco-2 cells. KSRP was regulated by the NF-κB signaling pathway and participated in mediating the innate immune response to S. enteritidis infection in Caco-2 cells, and KSRP acted as a negative regulator of inflammatory gene expression.

14.
Med Sci Monit ; 24: 2252-2258, 2018 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-29656299

RESUMO

BACKGROUND Our study explored the influences of hydration conditions and loading methods on the mechanical properties of cortical bones and cancellous bones. MATERIAL AND METHODS Elastic modulus and hardness of human cortical bones and cancellous bones that contained different moisture levels (20%, 30%, 40%, 50%, and 60%) were measured with nanoindentation with different peak loads and loading rates. Cortical bones with 20% and 60% moisture were tested with 30 nm, 40 nm, and 50 nm peak loads at 6 nm/s, 8 nm/s, and 10 nm/s loading rates, respectively. Cancellous bones with 5% or 40% moisture percentages were tested with 600 µN, 750 µN, and 1000 µN peak loads at 200 µN/s, 250 µN/s, and 333 µN/s loading rates, respectively. RESULTS Under the same loading condition, specimens with higher moisture contents showed decreased elastic modulus and hardness. Under different loading conditions, the loading modes had little influence on elastic modulus and hardness of cortical bone and cancellous bone with low moisture, but had significant influence on specimens with higher moistures. CONCLUSIONS The elastic modulus and bone hardness were affected by the moisture content and the loading conditions in cortical and cancellous bones with high hydration condition but not in those with low hydration condition.


Assuntos
Osso Esponjoso/fisiologia , Osso Cortical/fisiologia , Fenômenos Biomecânicos/fisiologia , Água Corporal/metabolismo , Água Corporal/fisiologia , Osso e Ossos/fisiologia , Osso Esponjoso/patologia , Osso Cortical/patologia , Módulo de Elasticidade/fisiologia , Dureza/fisiologia , Humanos , Análise Espectral , Suporte de Carga/fisiologia
15.
PLoS One ; 13(1): e0191101, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29370222

RESUMO

This article addresses the tradeoff between the losses caused by a new virus and the size of the team for developing an antivirus against the virus. First, an individual-level virus spreading model is proposed to capture the spreading process of the virus before the appearance of its natural enemy. On this basis, the tradeoff problem is modeled as a discrete optimization problem. Next, the influences of different factors, including the infection force, the infection function, the available manpower, the alarm threshold, the antivirus development effort and the network topology, on the optimal team size are examined through computer simulations. This work takes the first step toward the tradeoff problem, and the findings are instructive to the decision makers of network security companies.


Assuntos
Segurança Computacional , Admissão e Escalonamento de Pessoal , Simulação por Computador , Humanos , Risco
16.
Phys Chem Chem Phys ; 20(5): 3602-3607, 2018 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-29340380

RESUMO

Adsorption of short peptides, including three dipeptides: arginine-glycine (Arg-Gly), glycine-aspartic acid (Gly-Asp), arginine-aspartic acid (Arg-Asp), and one tripeptide arginine-glycine-aspartic acid (RGD), on the surfaces of Mg and Mg alloys (Mg-Zn, Mg-Y, and Mg-Nd), was studied using the first-principles calculations based on density functional theory (DFT), considering van der Waals (vdW) correction. The calculated adsorption energies (Eads) of short peptides on the clean Mg(0001) surface are in the range of -1.73 to -2.80 eV per dipeptide, and -3.24 eV for RGD. The short peptides prefer to bond to Mg atoms at the surface by the O and N anions in their functional groups. For the clean Mg(0001) surface, the Eads of the short peptides are exclusively dominated by the number of functional groups binding to the surface. However, for the surface of the Mg-Zn alloy (1% Zn), the adsorption of the peptides is clearly enhanced (by about 0.3 eV per peptide) due to the enhanced N-Mg bond and the electrostatic interactions between the doped Zn at the surface and the backbone chains of the peptides. Furthermore, the attractive interactions are increased with the increase of doped Zn contents (up to 3%). In contrast, for the surfaces of Mg-Y (1% Y) and Mg-Nd (1% Nd) alloys, the adsorption of the peptides is slightly weakened compared to that on the clean Mg(0001) surfaces. Our results provide useful guidance in understanding the interactions between peptides and the Mg-based biomedical alloy surfaces at the atomic scale in the biomimetic coating fields.


Assuntos
Ligas/química , Dipeptídeos/química , Magnésio/química , Oligopeptídeos/química , Adsorção , Dipeptídeos/metabolismo , Oligopeptídeos/metabolismo , Teoria Quântica , Eletricidade Estática , Propriedades de Superfície
17.
Neurochem Res ; 43(3): 659-668, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29340845

RESUMO

Neuroactive steroids such as progesterone, testosterone, and their derivatives have been widely studied for their neuroprotective roles in the nervous system. Autologous nerve transplantation is considered as the gold standard repair technique when primary suture is impossible; nevertheless, this method is far from ideal. In this study, we aimed to explore the impact of dihydrotestosterone (DHT), a 5α-reduced derivative of testosterone, on the recovery of peripheral nerve injury treated with autologous nerve transplantation. Sprague-Dawley rats were subjected to a 10-mm right side sciatic nerve reversed autologous nerve transplantation and randomly divided into groups that received DHT or DHT + flutamide (an androgen receptor blocker) daily for 8 weeks after operation. Our results demonstrated that DHT could speed up the rate of axonal regeneration and increase the expression of myelin protein zero (P0) in autograft reversal sciatic nerves. Thus, our study provided new insights into improving the prognosis of patients with long gap peripheral nerve defects.


Assuntos
Autoenxertos/efeitos dos fármacos , Di-Hidrotestosterona/farmacologia , Regeneração Nervosa/efeitos dos fármacos , Nervo Isquiático/efeitos dos fármacos , Animais , Modelos Animais de Doenças , Masculino , Proteína P0 da Mielina/metabolismo , Regeneração Nervosa/fisiologia , Traumatismos dos Nervos Periféricos/metabolismo , Traumatismos dos Nervos Periféricos/terapia , Ratos Sprague-Dawley , Nervo Isquiático/metabolismo , Nervo Isquiático/cirurgia
18.
Cytokine ; 106: 80-88, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29111086

RESUMO

BACKGROUND: Several studies suggest IL-17 is involved in the pathogenesis of organ fibrosis. The exact role of IL-17 in renal interstitial fibrosis has not been fully elucidated. METHODS: We compared the histopathology of renal fibrosis as well as profibrotic TGF-ß signaling in wild-type (WT) and IL-17 knock-out (IL-17-/-) mice using UUO as the disease model. To find out the possible mechanisms involved in the exacerbated renal fibrosis happened to IL-17-/- mice, we analyzed the pattern of ECM synthesis by different fibroblasts cultured with IL-17 and associated signaling mediators. RESULTS: On day3 and day7, IL-17-/- mice developed more severe renal fibrosis compared with WT mice. IL-17 had an inhibitory factor in TGF-ß-induced renal fibroblast activation and ECM synthesis, and sequentially in renal interstitial fibrosis, via down-regulation of Smad -independent pathway (p38MAPK and AKT phosphorylations). CONCLUSION: IL-17 acts an inhibitory factor in TGF-ß-induced renal fibroblast activation and ECM synthesis, and sequentially in renal interstitial fibrosis, via down-regulation of Smad-independent pathway (p38MAPK and AKT phosphorylations). Clarifying the novel regulatory mechanisms of fibrosis by the cytokine IL-17 may lead to a new therapeutic approach for progressive renal disease and fibrosis.

19.
J Biomed Res ; 32(2): 113-122, 2018 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-28963441

RESUMO

Systemic lupus erythematosus (SLE) is a typical autoimmune disease. Lymphotoxin ß receptor (LTßR) signaling plays an important role in autoimmune inflammations. LTßR-Ig fusion protein, LTßR blocking agent, has been used to treat SLE, while its mechanism remains to be fully elucidated. In this study, to investigate the expression of LTßR in the T cells of SLE patients and its roles in the pathogenesis of SLE, we isolated the peripheral blood T cells of SLE patients and normal controls to detect expression of LTßR by flow cytometry and RNA assay. T cells were also stimulated with LIGHT, a ligand of LTßR, and then detected for their LTßR expressions and apoptosis by flow cytometry. Also, their expressions of inflammatory factors and receptors were determined by RNA assay. The results showed that LTßR positive cells were 22.75%±6.98% in CD3+ cells of SLE patients, while there were almost no LTßR positive cells in CD3+ cells of normal persons. Moreover, LTßR expression was remarkably higher in CD3, CD4 and CD8 positive T cells of active SLE patients than non/low active patients (all P<0.05), and positively correlated with increased Ig level, decreased complement level and renal damage. Moreover, the stimulation of SLE T cells with LIGHT promoted higher expression of LTßR, IL-23R and IL-17A, and apoptosis of T cells. In conclusion, we demonstrated a high expression of LTßR in the T cells of SLE patients which may be associated with pathogenesis of SLE.

20.
J Sci Food Agric ; 98(3): 1222-1231, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28755408

RESUMO

BACKGROUND: Fatty acids and amino acids are the precursors of aliphatic and aromatic volatile compounds, higher alcohols and esters. They are also nutrition for yeast metabolism during fermentation. However, few reports have been concerned about the effect of viticulture practices on the accumulation of fatty acids and amino acids in wine grapes. This study aimed to explore the accumulation of these compounds in developing Vitis vinifera L. cv. Chardonnay grape berries under two vintages, and compare the influences of the rain-shelter cultivation and open-field cultivation. RESULTS: Fifteen fatty acids and 21 amino acids were detected in total. The rain-shelter cultivation led to an increase in the total concentration of fatty acids, and a decrease in the total concentration of amino acids compared with the open-field cultivation in 2012, while no significant difference was observed between two cultivation modes in 2013 vintage. Concentrations of palmitoleic acid, isoleucine and cysteine were significantly promoted in the rain-shelter grape berries, whereas those of tyrosine and ornithine were markedly reduced in both vintages. CONCLUSION: The rain-shelter cultivation of wine grapes in the rainy region is beneficial for improving grape quality and fermentation activity by influence on the concentration of fatty acids and amino acids. © 2017 Society of Chemical Industry.


Assuntos
Aminoácidos/metabolismo , Ácidos Graxos/metabolismo , Frutas/química , Vitis/metabolismo , Aminoácidos/química , Ácidos Graxos/química , Frutas/crescimento & desenvolvimento , Frutas/metabolismo , Chuva , Vitis/química , Vitis/crescimento & desenvolvimento , Vinho/análise
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