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1.
Bioorg Chem ; 101: 104032, 2020 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-32599370

RESUMO

The aim of this study was to effectively obtain monoamine oxidase A (MAO-A) inhibitory peptides from in vitro simulated gastrointestinal digestion and to assess the correspondences between in silico prediction and in vitro confirmation. Fractions (<3 kDa) from ultrafiltration of pepsin and simulated gastrointestinal enzymes hydrolysates exhibited the highest MAO-A inhibitory activity with IC50 values of 0.61 and 2.54 mg/mL, respectively. After sequencing and then screening by HPEPDOCK, 11 high-score peptides and 2 low-score peptides were selected for further synthesis. Remarkable correlation was found between (-)docking scores and MAO-A inhibitory activity of the synthesized peptides, and among which VVFEVFW showed the highest MAO-A inhibitory activity (IC50 = 0.405 mM). Current research suggested that in silico is an effective method to screen MAO-A inhibitory peptides from hairtail protein hydrolysates, and these peptides can be used as functional ingredients for MAO-A inhibition or potential alternatives for antidepressant.

2.
Ultrasound Med Biol ; 2020 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-32591167

RESUMO

The aim of this study was to explore the value of transcutaneous laryngeal ultrasonography in the diagnosis of vocal fold polyps. From December 2016 to June 2019, 87 patients with vocal fold polyps diagnosed pathologically in the Otolaryngology Head and Neck Surgery Department of the Second Affiliated Hospital of Xi'an Jiaotong University were enrolled and examined by electronic laryngoscopy and percutaneous laryngeal ultrasound on the same day before operation. To observe the effect of calcification length as a percentage of thyroid cartilage at the glottic level on vocal fold display, the characteristics of ultrasound images of vocal fold polyps and the value of transcutaneous laryngeal ultrasonography in the diagnosis of vocal fold polyps were assessed. Among 87 patients, the calcification rate of thyroid cartilage at the glottic level was 33.3%. The differences in calcification rate and percentage of calcification length between males and females were statistically significant. The rate of detection of vocal folds decreased gradually with an increase in calcification length percentage. Imaging features of vocal fold polyps were hypo-echoic with a clear boundary and regular shape. The detection rates for circular and non-circular polyps were 92.0% and 70.6%. Ultrasound was more likely to detect circular than non-circular polyps; however, the difference was not significant. Transcutaneous laryngeal ultrasonography can identify the morphology and location of vocal fold polyps and is non-invasive and highly accurate. Therefore, it has the potential to be an effective supplement to laryngoscopy for initial screening and post-operative review of vocal fold polyps.

3.
Signal Transduct Target Ther ; 5(1): 75, 2020 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-32499481

RESUMO

The activation of hepatic stellate cells (HSCs) participates in liver fibrosis, and emerging evidences indicate that microRNAs (miRNAs) are abnormally expressed during HSC activation. However, the potential roles of miRNAs in liver fibrosis still remain elusive. Therefore, this study aimed to investigate the role of miR-199a-3p in liver fibrosis and its underlying mechanism. We found that miR-199a-3p expression was dramatically upregulated during HSC activation in vitro, and during liver fibrogenesis in CCl4-treated rats, and its liver expression was increased in the patients with cirrhosis. By the luciferase assay and RT-qPCR, we revealed that the expression of miR-199a-3p in HSCs was driven by the transcription factor Twist1 which could be further induced by TGF-ß treatment. Functional studies showed that inhibition of miR-199a-3p in both human LX2 cells and rat HSCs significantly decreased the expression of fibrotic markers, such as fibronectin and connective tissue growth factor (CTGF), whereas the forced expression of miR-199a-3p exhibited opposite effects, demonstrating the role of miR-199a-3p in promoting HSC activation. Mechanistically, miR-199a-3p plays an important role in TGF-ß signalling pathway activation through targeting CAV2 that negatively regulates the expression of transforming growth factor-beta receptor type I (TGFßRI). Importantly, administration of antagomiR-199a-3p in the CCl4-treated mice significantly ameliorated hepatic fibrosis. In conclusion, Twist1-induced miR-199a-3p mediates the activation of HSCs by suppressing CAV2 expression and subsequently increasing TGFßRI expression to promote TGF-ß pathway. Our findings highlight the therapeutic potential of miR-199a-3p for hepatic fibrosis.

4.
Plant Cell Rep ; 2020 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-32333150

RESUMO

KEY MESSAGE: MsCBL4 expression in tobacco enhanced its salt and saline-alkali stress tolerance by regulating calcium accumulation in roots, indicating the important role of calcium metabolism in plant saline-alkali stress tolerance The calcineurin B-like (CBL) family of proteins play important roles in plant abiotic stress tolerance and signal transduction. CBL4 is known to participate in the Salt Overly Sensitive pathway; however, little is currently known regarding the mechanisms underlying the response of CBL4 to saline-alkali stress. In this study, we cloned and characterized the alfalfa MsCBL4 gene. We found that MsCBL4 showed the highest expression in root tissues and was induced by salt and saline-alkali stress, with the latter causing higher induction. Overexpression of MsCBL4 in tobacco enhanced salt and saline-alkali stress tolerance and reduced the Na+/K+ ratio in roots of transgenic lines. Salt (30 and 300 mM NaCl) and saline-alkali (30 mM NaHCO3) stress assays performed for MsCBL4 transgenic tobacco lines revealed a substantial influx of sodium ions in roots under saline-alkali stress and indicated that the expression of MsCBL4 had little influence on sodium ion content reduction. In contrast, in roots subjected to saline-alkali stress, calcium accumulation occurred and was significantly enhanced by the overexpression of MsCBL4. Physiological and biochemical analyses indicated that MsCBL4 plays an important role in saline-alkali stress tolerance via its influence on the regulation of calcium transport and accumulation. These results provide novel insights into the saline-alkali stress tolerance mechanisms of plants.

5.
Hypertension ; 75(6): 1532-1541, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32306769

RESUMO

Dysregulated RNA editing is well documented in several diseases, such as cancer and neurodegenerative diseases. The extent to which RNA editing might be involved in diseases originated in the placenta remains unknown. Here, we have systematically profiled RNA editome on the placentae, 9 from patients with early-onset severe preeclampsia (EOSPE) and 32 from normal subjects, and a widespread RNA editing dysregulation in EOSPE has been identified. The mis-edited gene set is enriched with known preeclampsia-associated genes and differentially expressed genes in EOSPE. The RNA editing events at 2 microRNA binding sites in 3'-untranslated region of the LEP mRNA were generated, which could inhibit the microRNA-induced mRNA downregulation of LEP in placenta-derived cell line, consistent with the observation in the placentae of preeclampsia patients. These results demonstrate the association of dysregulated placental RNA editing with preeclampsia, and providing a resource for further study on the role of RNA editing in the pathogenesis of this disease.

6.
Biomed Res Int ; 2020: 8701759, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32185222

RESUMO

Objectives: To access the distinct values of contrast transcranial Doppler (cTCD), contrast transthoracic echocardiography (cTTE), and contrast transesophageal echocardiography (cTEE) in the diagnosis of right-to-left shunt (RLS) due to patent foramen ovale (PFO) and to define the most practical strategy for the diagnosis of PFO. Methods: 102 patients with a high clinical suspicion for PFO had simultaneous cTCD, cTTE, and cTEE performed. The agitated saline mixed with blood was used to detect right-to-left shunt (RLS). Results: In all 102 patients, the shunt was detected at rest by cTCD in 60.78% of cases, by cTTE in 42.16%, and by cTEE in 47.06%. The positive results of all 3 techniques with Valsalva maneuver (VM) were significantly improved. cTCD showed higher pick-up rate than cTTE (98.04% vs. 89.22%; χ 2 = 12.452, p < 0.05) and the cTEE (98.04% vs. 96.08%; nonsignificant difference) in the diagnosis of PFO. Nevertheless, cTEE, compared with cTTE, underestimated shunting in 44% of patients. The diameter of both PFO entrance and exit was significantly greater in patients with a severe shunt compared with a mild shunt (2.8 ± 1.0 mm vs. 2.0 ± 0.7 mm, t = 3.135, p < 0.05) and the cTEE (98.04% vs. 96.08%; nonsignificant difference) in the diagnosis of PFO. Nevertheless, cTEE, compared with cTTE, underestimated shunting in 44% of patients. The diameter of both PFO entrance and exit was significantly greater in patients with a severe shunt compared with a mild shunt (2.8 ± 1.0 mm vs. 2.0 ± 0.7 mm, t = 3.135, p < 0.05) and the cTEE (98.04% vs. 96.08%; nonsignificant difference) in the diagnosis of PFO. Nevertheless, cTEE, compared with cTTE, underestimated shunting in 44% of patients. The diameter of both PFO entrance and exit was significantly greater in patients with a severe shunt compared with a mild shunt (2.8 ± 1.0 mm vs. 2.0 ± 0.7 mm, t = 3.135, p < 0.05) and the cTEE (98.04% vs. 96.08%; nonsignificant difference) in the diagnosis of PFO. Nevertheless, cTEE, compared with cTTE, underestimated shunting in 44% of patients. The diameter of both PFO entrance and exit was significantly greater in patients with a severe shunt compared with a mild shunt (2.8 ± 1.0 mm vs. 2.0 ± 0.7 mm. Conclusions: The best method to diagnose PFO should be the combination of cTCD, cTTE, and cTEE. And cTCD should be applied as the first choice for screening RLS. Then, cTTE should be performed to quantify the severity of the shunt. Last but not least, cTEE should be performed to assess the morphologies of PFO when the closure is planned. The study provides for clinicians the most practical strategy for diagnosing PFO in the future. However, further trials with a large sample size are required to confirm this finding.

7.
Mol Autism ; 11(1): 13, 2020 02 07.
Artigo em Inglês | MEDLINE | ID: mdl-32033586

RESUMO

Autism spectrum disorder (ASD) is a neuronal developmental disorder with impaired social interaction and communication, often with abnormal intelligence and comorbidity with epilepsy. Disturbances in synaptic transmission, including the GABAergic, glutamatergic, and serotonergic systems, are known to be involved in the pathogenesis of this disorder, yet we do not know if there is a common molecular mechanism. As mutations in the GABAergic receptor subunit gene GABRA4 are reported in patients with ASD, we eliminated the Gabra4 gene in mice and found that the Gabra4 knockout mice showed autistic-like behavior, enhanced spatial memory, and attenuated susceptibility to pentylenetetrazol-induced seizures, a constellation of symptoms resembling human high-functioning autism. To search for potential molecular pathways involved in these phenotypes, we performed a hippocampal transcriptome profiling, constructed a hippocampal interactome network, and revealed an upregulation of the NMDAR system at the center of the converged pathways underlying high-functioning autism-like and anti-epilepsy phenotypes.

8.
Food Chem ; 295: 569-578, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31174797

RESUMO

Fish scales are usually discarded or used to produce fish meal, etc. In order to enhance their utility, we produced the gelatin hydrolysates from fish scales (FSGH) and they were heated with glucose, xylose, and ribose to prepare sugar-FSGH Maillard reaction products (MRPs). The antioxidant capacity and sensory property of MRPs were evaluated. The results showed that ribose-FSGH MRPs exhibited higher antioxidant capacity than glucose- and xylose-FSGH MRPs. After simulated gastrointestinal digestion, the DPPH and ABTS scavenging activity of ribose-FSGH MRPs were 25.32 µM and 193.37 µM Trolox equivalent/g sample, respectively, and the reducing power was 0.509. Flavor compounds (such as butanal, benzaldehyde, 2-methyl-3-furanthiol, and maltol) of ribose-FSGH MRPs were produced in abundance after 5 h of heating and ribose-FSGH MRPs exhibited flavor enhanced effect on caramel-like and mouthfulness sensory attributes. These results suggest that ribose-FSGH MRPs can be potentially used as food antioxidants.


Assuntos
Antioxidantes/química , Carpas/metabolismo , Gelatina/metabolismo , Produtos Finais de Glicação Avançada/química , Compostos Orgânicos Voláteis/análise , Aminoácidos/análise , Animais , Cromatografia Líquida de Alta Pressão , Aromatizantes/química , Aromatizantes/metabolismo , Cromatografia Gasosa-Espectrometria de Massas , Concentração de Íons de Hidrogênio , Alimentos Marinhos/análise , Temperatura
9.
J Hazard Mater ; 376: 1-11, 2019 08 15.
Artigo em Inglês | MEDLINE | ID: mdl-31096108

RESUMO

Focused on the removal of the complicated residual antibiotic in aqueous environment, in this work, a novel carbon dots (C-dots) sensitized 2D-2D heterojunction of BiVO4/Bi3TaO7 were assembled through a simple hydrothermal process. The characteristic by TEM, SEM, and XPS confirmed C-dots evenly anchored on the surface of BiVO4/Bi3TaO7 heterojunction. The as-prepared C-dots/BiVO4/Bi3TaO7 showed superior performance for the degradation of the various antibiotics under visible light illumination. When the concentration of C-dots in the composite is 3 wt.%, the photodegraded rates are obtained to be 91.7%, 89.3%, 87.1%, for tetracycline (TC), amoxicillin (AMX) and ciprofloxacin (CIP), respectively, without significant deactivation during consecutive ten recycle experiments. Furthermore, by assessing the antibiotics mixture solution of TC, AMX and CIP, it is proposed that the prepared samples are potentially effective for the wastewater effluents. A probable mechanism was reasonably proposed. The improved photocatalytic activities could be attributed to the unique construction of the C-dots mediated heterojunction, which could expedite electron migration, improve light harvesting capacity and enhance charge separation efficiency. The present investigation may provide a new perspective to design C-dots mediated heterojunction which could be a potential visible-light-driven photocatalysts for the better practical applications in remediation of broad-spectrum antibiotic residues.

10.
Nat Protoc ; 14(3): 795-818, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30700807

RESUMO

Abundant long, noncoding RNAs (lncRNAs) in mammals can bind to DNA sequences and recruit histone- and DNA-modifying enzymes to binding sites to epigenetically regulate target genes. However, most lncRNAs' binding motifs and target sites are unknown. The large numbers of lncRNAs and target sites in the whole genome make it infeasible to examine lncRNA binding to DNA purely experimentally. Here, we report a protocol for lncRNA/DNA-binding analysis that is built upon a database containing the GENCODE-annotated human and mouse lncRNAs, the orthologs of these lncRNAs in 17 mammals, and the genome sequences of the 17 mammals. Cross-species and genome-wide lncRNA/DNA-binding analysis begins with and is driven by database search. The predicted DNA-binding motifs and binding sites answer the general question of which lncRNAs may epigenetically regulate which genes, and can be used to identify potential sites for genome and epigenome editing. To use the protocol, preliminary knowledge of the base-pairing rules that guide the binding of noncoding RNAs to DNA to form triplexes, as well as the skills required to use the UCSC Genome Browser, are needed. A genome-wide prediction takes from 2 to 10 d, and the results are sent to users automatically by e-mail. The platform is updated continuously, making it possible to study more lncRNAs and larger genomic regions in less computational time.


Assuntos
Biologia Computacional/métodos , Genoma , RNA Longo não Codificante/metabolismo , Animais , Sítios de Ligação , Humanos , Mamíferos/genética , Camundongos , Oligonucleotídeos/metabolismo , Filogenia , Especificidade da Espécie
11.
Virology ; 528: 101-109, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30590261

RESUMO

Duck virus hepatitis (DVH) caused by duck hepatitis A virus type 1 (DHAV-1) is an acute and highly contagious disease affecting young ducklings. The VP1 protein is one of the major structural proteins of DHAV-1 carries critical epitopes responsible for the induction of neutralizing antibodies. In this study, we have successfully constructed an immune phage display VHHs library against DHAV-1 with the size of 6 × 106 colonies. A nanobody (Nb) against VP1 protein of DHAV-1, named Nb25, was identified from the immunized phage display library. Nb25 could react with the conserved linear B-cell epitope of 174PAPTST179 in DHAV-1 VP1, even though Nb25 showed no neutralizing activity to DHAV-1. To the best of our knowledge, this is the first report about preparation of anti-DHAV-1 Nbs and identification of the specific conserved linear B-cell epitope of DHAV-1 with Nb, which will facilitate the serologic diagnosis of DHAV-1 infection.


Assuntos
Epitopos de Linfócito B/imunologia , Vírus da Hepatite do Pato/imunologia , Anticorpos de Domínio Único/isolamento & purificação , Proteínas Estruturais Virais/imunologia , Animais , Técnicas de Visualização da Superfície Celular , Patos , Hepatite Viral Animal/diagnóstico , Hepatite Viral Animal/imunologia , Doenças das Aves Domésticas/virologia
12.
Cell Death Dis ; 9(7): 718, 2018 06 18.
Artigo em Inglês | MEDLINE | ID: mdl-29915227

RESUMO

MicroRNAs (miRNAs) have been demonstrated to modulate cellular processes in the liver. However, the role of miRNAs in liver fibrosis is poorly understood. Because the activation of hepatic stellate cells (HSCs) is a pivotal event in the initiation and progression of hepatic fibrosis, we investigate the differential expression of miRNAs in activated and quiescent rat HSCs by microarray analysis and find that miR-214 (miR-214-3p) is significantly upregulated during HSC activation. Moreover, the robust induction of miR-214 is correlated with liver fibrogenesis in carbon tetrachloride (CCl4)-treated rats and mice, high-fat diet-induced non-alcoholic steatohepatitis in mice, and cirrhosis in humans. We identify that miR-214 expression is driven by the helix-loop-helix transcription factor Twist1 via the E-box element. The increased miR-214 inhibits the expression of suppressor-of-fused homolog (Sufu), a negative regulator of the Hedgehog signaling pathway, thereby contributing to HSC activation to promote the accumulation of fibrous extracellular matrix and the expression of profibrotic genes in HSCs and LX2 cells. Furthermore, miR-214 expression is inversely correlated with the expression of Sufu in clinical cirrhosis samples. To explore the clinical potential of miR-214, we inject antagomiR-214 oligos into mice to induce hepatic fibrosis. The knockdown of miR-214 in vivo enhances Sufu expression and reduces fibrosis marker expression, which ameliorates liver fibrosis in mice. In conclusions, the Twist1-regulated miR-214 promotes the activation of HSC cells through targeting Sufu involved in the Hedgehog pathway and participates in the development of hepatic fibrosis. Hence, the knockdown of miR-214 expression may be a promising therapeutic strategy for liver fibrosis.


Assuntos
Células Estreladas do Fígado/fisiologia , Cirrose Hepática/genética , MicroRNAs/fisiologia , Proteínas Repressoras/genética , Animais , Proliferação de Células/genética , Células Cultivadas , Regulação para Baixo/genética , Células Estreladas do Fígado/patologia , Humanos , Cirrose Hepática/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Ratos , Ratos Sprague-Dawley , Proteínas Repressoras/metabolismo , Transdução de Sinais/genética
13.
Biofouling ; 34(5): 545-556, 2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-29842799

RESUMO

The bryozoan Bugula neritina is a cosmopolitan marine fouling species that causes major fouling problems in sub-tropical waters. Settlement of B. neritina larvae can be triggered without an obvious external cue. Here, the negative regulatory role of nitric oxide (NO) during larval settlement of B. neritina was demonstrated to be mediated by cyclic guanosine monophosphate (cGMP). Although the regulatory role of the NO-p38 MAPK signaling axis in larval settlement was not evident, inhibition of nitric oxide synthase (NOS) led to the deactivation of p38 MAPK. Exclusive localization of NO and NO signaling components in sensory-related organs of the larvae is consistent with its signal transduction function in metamorphosis. Overall, this study provides new insights into the regulatory roles of the NO-p38MAPK/cGMP pathway in B. neritina settlement.


Assuntos
Biofilmes/crescimento & desenvolvimento , Briozoários/fisiologia , GMP Cíclico/metabolismo , Larva/fisiologia , Óxido Nítrico/metabolismo , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo , Animais , Incrustação Biológica , Briozoários/metabolismo , Larva/metabolismo , Metamorfose Biológica , Transdução de Sinais
14.
Cell Physiol Biochem ; 46(3): 1241-1251, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29672295

RESUMO

BACKGROUND/AIMS: Autoimmune hepatitis (AIH) is a chronic necroinflammatory disease of the liver whose pathogenic mechanisms have not yet been elucidated. Moreover, the current treatment used for the vast majority of AIH patients is largely dependent on immunosuppressant administration and liver transplantation. However, research on the pathogenesis of AIH and effective new treatments for AIH have been hampered by a lack of animal models that accurately reproduce the human condition. METHODS: AIH models created by concanavalin A (ConA) injections at different times and doses. The levels of ALT, AST, LDH and inflammatory cytokines were examined at various times after 20 mg/kg ConA was administered by ELISA using commercially available kits. Moreover, liver pathological changes were observed by flow cytometry (FCM) and H&E staining. RESULTS: Our experiments demonstrated that the levels of ALT, AST, LDH and several inflammatory cytokines, including TNF-α, IFN-γ, and IL-6, were higher in the 20 mg/kg 12 h ConA group than in the other groups. Importantly, the numbers of activated CD4+ and CD8+ T lymphocytes in the blood, spleen and liver were calculated. These results showed that ConA (20 mg/kg for 12 h)-induced hepatitis was similar to that in clinical AIH patients. Furthermore, we found that the number of MDSCs in the blood was significantly increased in the ConA (20 mg/kg for 12 h) group compared with controls. Our findings indicated that ConA (20 mg/kg for 12 h)-induced hepatitis could be used as an experimental murine model that mirrors most of the pathogenic properties of human type I AIH. CONCLUSION: This model [ConA (20 mg/kg for 12 h)] provides a valuable tool for studying AIH immunopathogenesis and rapidly assessing novel therapeutic approaches.


Assuntos
Concanavalina A/toxicidade , Hepatite Autoimune/patologia , Adulto , Alanina Transaminase/sangue , Animais , Antígenos CD/metabolismo , Antígenos de Diferenciação de Linfócitos T/metabolismo , Aspartato Aminotransferases/sangue , Linfócitos T CD4-Positivos/citologia , Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD4-Positivos/metabolismo , Linfócitos T CD8-Positivos/citologia , Linfócitos T CD8-Positivos/imunologia , Linfócitos T CD8-Positivos/metabolismo , Modelos Animais de Doenças , Feminino , Hepatite Autoimune/etiologia , Hepatite Autoimune/metabolismo , Humanos , Interleucina-4/metabolismo , Interleucina-6/metabolismo , Lectinas Tipo C/metabolismo , Fígado/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Pessoa de Meia-Idade , Fator de Necrose Tumoral alfa/metabolismo
15.
J Exp Biol ; 221(Pt 8)2018 04 23.
Artigo em Inglês | MEDLINE | ID: mdl-29487161

RESUMO

The larvae of many sessile marine invertebrates go through a settlement process, during which planktonic larvae attach to a substrate and metamorphose into sessile juveniles. Larval attachment and metamorphosis (herein defined as 'settlement') are complex processes mediated by many signalling pathways. Nitric oxide (NO) signalling is one of the pathways that inhibits larval settlement in marine invertebrates across different phyla. NO is synthesized by NO synthase (NOS), which is a client of the molecular chaperone heat shock protein 90 (HSP90). In the present study, we provide evidence that NO, a gaseous messenger, regulates larval settlement of Bugula neritina By using pharmacological bioassays and western blotting, we demonstrated that NO inhibits larval settlement of B. neritina and that NO signals occur mainly in the sensory organ of swimming larvae. The settlement rate of B. neritina larvae decreased after heat shock treatment. Inhibition of HSP90 induced larval settlement, and attenuated the inhibition of NO donors during larval settlement. In addition, the expression level of both HSP90 and NOS declined upon settlement. These results demonstrate that HSP90 regulates the larval settlement of B. neritina by interacting with the NO pathway.


Assuntos
Briozoários/crescimento & desenvolvimento , Proteínas de Choque Térmico HSP90/metabolismo , Óxido Nítrico/metabolismo , Sequência de Aminoácidos , Animais , Benzoquinonas/administração & dosagem , Briozoários/efeitos dos fármacos , Inibidores Enzimáticos/administração & dosagem , Proteínas de Choque Térmico HSP90/genética , Lactamas Macrocíclicas/administração & dosagem , Larva/efeitos dos fármacos , Larva/crescimento & desenvolvimento , Metamorfose Biológica/efeitos dos fármacos , Óxido Nítrico Sintase/metabolismo , Transdução de Sinais
16.
Biomed Pharmacother ; 100: 213-220, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29428670

RESUMO

Autoimmune hepatitis (AIH) is a chronic inflammatory liver disease of an unknown etiology, glucocorticoid therapy is currently recognized as an effective treatment for AIH, but conventional application and patient compliance are both hindered by its side effects. The exploration of the AIH pathogenesis and the searching for the new candidate drugs that exert potential activity and low toxicity are urgently needed. Pomegranate peel extract (PoPx) is a natural extract of Punica granatum and has been reported to have anti-inflammatory and antioxidative properties. The present study aimed to clarify the effect of PoPx on the concanavalin A (ConA)-induced autoimmune hepatitis in a mouse model that is well established at 12h after tail vein injection with a dose of 20 mg/kg of ConA. C57BL/6 female mice were pretreated with PoPx (250 mg/kg, once daily for 3 days) followed by a ConA challenge. Pretreatment with PoPx significantly alleviated ConA-induced liver injury by down-regulating the levels of plasma alanine transaminase (ALT), aspartate transaminase (AST) and cytokine, including TNF-α, interferon (IFN) -γ and interleukin (IL)-6. Moreover, liver hematoxylin and eosin (H&E) staining displayed a lighter inflammatory infiltration around the portal area in the PoPx-pretreated mice. In addition, the flow cytometry (FCM) data showed that the immune response in the liver was died down in the PoPx-pretreated condition. Specially, pretreatment with PoPx reduced the infiltration of activated CD4+ and CD8+ T cells in the liver. Taken together, these findings contributed to a better understanding of the actions of PoPx against acute AIH and indicated that PoPx might be a potential compound in treating T cell-mediated autoimmune liver injury.


Assuntos
Concanavalina A/imunologia , Medicamentos de Ervas Chinesas/uso terapêutico , Frutas/química , Hepatite Autoimune/prevenção & controle , Fígado/efeitos dos fármacos , Lythraceae/química , Animais , Modelos Animais de Doenças , Medicamentos de Ervas Chinesas/isolamento & purificação , Feminino , Hepatite Autoimune/imunologia , Hepatite Autoimune/patologia , Fígado/enzimologia , Fígado/patologia , Testes de Função Hepática , Medicina Tradicional Chinesa , Camundongos Endogâmicos C57BL
17.
Biol Open ; 7(1)2018 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-29242196

RESUMO

Marine biofouling poses a severe threat to maritime and aquaculture industries. To prevent the attachment of marine biofouling organisms on man-made structures, countless cost and effort was spent annually. In particular, most attention has been paid on the development of efficient and environmentally friendly fouling-resistant coatings, as well as larval settlement mechanism of several major biofouling invertebrates. In this study, polydimethylsiloxane (PDMS) micropost arrays were utilized as the settlement substrata and opposite tractions were identified during early settlement of the barnacle Amphibalanus amphitrite and the bryozoan Bugula neritina The settling A. amphitrite pushed the periphery microposts with an average traction force of 376.2 nN, while settling B. neritina pulled the periphery microposts with an average traction force of 205.9 nN. These micropost displacements are consistent with the body expansion of A. amphitrite during early post-settlement metamorphosis stage and elevation of wall epithelium of B. neritina during early pre-ancestrula stage, respectively. As such, the usage of micropost array may supplement the traditional histological approach to indicate the early settlement stages or even the initiation of larval settlement of marine fouling organisms, and could finally aid in the development of automatic monitoring platform for the real-time analysis on this complex biological process.

18.
Sci Rep ; 7(1): 15779, 2017 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-29150661

RESUMO

Different rotifer stains exhibited remarkably morphological differences which could not be eliminated under laboratory conditions. In the present study, we hypothesized that predation pressure and pollution might be two forces driving morphological differentiation of rotifer. To test this hypothesis, rotifers (Brachionus calyciflorus) belonging to two sibling species were collected from three special lakes (with coal ash pollution, high predation pressure or neither) and cultured for more than three months to investigate their potential differentiation in morphology. Twelve morphological parameters were measured and compared among three lakes at four food density (Scenedesmus obliquus). The results showed that most of the tested morphological parameters changed in response to food level and differed among three habitats. Rotifers from the habitat with high predation pressure evolved stable long posterior lateral spine and relatively small body size. Rotifers collected from the polluted habitat was of smaller body size, compared with those from ordinary habitat. Bigger eggs were laid by rotifers from polluted area or lake with high predation pressure, enabling newborns more resistant to pollution or predation, and thus ensuring the survival rate of newborns. Finally, we concluded that both predation and pollution could affect the morphological differentiation and evolution of rotifers.


Assuntos
Cinza de Carvão/análise , Poluição Ambiental/análise , Comportamento Predatório/fisiologia , Rotíferos/citologia , Animais , Tamanho Corporal , Cadeia Alimentar , Lagos , Óvulo/citologia , Rotíferos/anatomia & histologia
19.
Life Sci ; 187: 22-30, 2017 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-28823564

RESUMO

AIM: To understand the contribution of miR-142-3p in the activation of hepatic stellate cells (HSCs) and liver fibrosis, and the underlying mechanism. MATERIALS AND METHODS: We detected microRNAs expression profiles in quiescent and activated HSCs by microRNA-array, and performed qRT-PCR to validate these data in HSCs and plasma of cirrhosis patients. In vitro, the 3rd-5th passage HSCs was transfected with mir-142-3p mimics or stimulated with TGF ß. The markers of HSCs activation (i.e. FN and α-SMA) were examined by qRT-PCR and western blotting, and cell viability was detected by MTT, colony formation assays respectively. KEY FINDING: In our study, we identified miR-142-3p as a novel regulator of HSCs activation and indicator of hepatic cirrhosis. We found that miR-142-3p was significantly reduced in activated HSCs, while TGFßRI was distinctly up-regulated in activated HSCs. Ectopic expression of miR-142-3p in activated HSCs inhibited cell viability as well as cell growth, and blocked HSCs activation, concomitant with decreased transdifferentiation markers (i.e. FN and α-SMA). Further, we confirmed that miR-142-3p was reduced upon TGF-ß exposure, while diminishing TGF-ß-Smad signaling pathway in turn by reducing TGFßRI expression in HSCs. Besides, the plasma level of miR-142-3p declined significantly in patients with hepatic cirrhosis. SIGNIFICANCE: In conclusion, we demonstrated that miR-142-3p repressed TGF-ß-Smad signaling pathway to prevent HSCs activation through directly targeting TGFßRI in HSCs.


Assuntos
Células Estreladas do Fígado/metabolismo , MicroRNAs/farmacologia , Fator de Crescimento Transformador beta/metabolismo , Animais , Estudos de Casos e Controles , Proliferação de Células/efeitos dos fármacos , Transdiferenciação Celular/efeitos dos fármacos , Humanos , Cirrose Hepática/sangue , Cirrose Hepática/metabolismo , Masculino , MicroRNAs/sangue , MicroRNAs/metabolismo , Cultura Primária de Células , Proteínas Serina-Treonina Quinases/biossíntese , Ratos , Receptor do Fator de Crescimento Transformador beta Tipo I , Receptores de Fatores de Crescimento Transformadores beta/biossíntese , Transdução de Sinais/efeitos dos fármacos , Proteína Smad2/metabolismo , Proteína Smad3/metabolismo , Fator de Crescimento Transformador beta/antagonistas & inibidores , Fator de Crescimento Transformador beta/farmacologia , Regulação para Cima
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