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1.
Pediatr Res ; 86(3): 305-310, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31117117

RESUMO

BACKGROUND: We previously demonstrated an association between placental leptin (LEP) methylation levels and macrosomia without gestational diabetes mellitus (non-GDM). This study further explored the association between LEP methylation in cord blood and non-GDM macrosomia. METHOD: We carried out a case-control study of 61 newborns with macrosomia (birth weight ≥4000 g) and 69 newborns with normal birth weight (2500-3999 g). Methylation in the LEP promoter region was mapped by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. RESULTS: Average cord blood LEP methylation levels were lower in macrosomia newborns than in control newborns (P < 0.001). Eleven CpG sites were associated with macrosomia. Multivariate logistic regression revealed that low LEP methylation levels [adjusted odds ratio (AOR) = 2.84, 95% confidence interval (CI): 1.72-4.17], high pre-pregnancy body mass index (AOR = 7.44, 95% CI: 1.99-27.75), long gestational age (AOR = 3.18, 95% CI: 1.74-5.79), high cord blood LEP concentration (AOR = 2.25, 95% CI: 1.34-3.77), and male newborn gender (AOR = 3.91, 95% CI: 1.31-11.69) significantly increased the risk of macrosomia. CONCLUSIONS: Lower cord blood LEP methylation levels and certain maternal and fetal factors are associated with non-GDM macrosomia.

2.
PLoS One ; 13(2): e0192361, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29466390

RESUMO

Rhabdomyolysis (RM) may cause kidney damage and results primarily in acute kidney injury (AKI). Complement is implicated in the pathogenesis of renal diseases and ischemia-reperfusion injury (IRI), but the role of complement, especially its activation pathway(s) and its effect in RM-induced AKI, is not clear. This study established a rat model of AKI induced by RM via intramuscular treatment with glycerol. Cobra venom factor (CVF) was administered via tail vein injection to deplete complement 12 h prior to intramuscular injection of glycerol. We found that the complement components, including complement 3 (C3), C1q, MBL-A, factor B(fB), C5a, C5b-9, and CD59, were significantly increased in rat kidneys after intramuscular glycerol administration. However, the levels of serum BUN and Cr, renal tubular injury scores, and the number of TUNEL-positive cells decreased significantly in the CVF+AKI group. These results suggest that complement plays an important role in RM-induced AKI and that complement depletion may improve renal function and decrease renal tissue damage by reducing the inflammatory response and apoptosis.


Assuntos
Lesão Renal Aguda/etiologia , Ativação do Complemento , Modelos Animais de Doenças , Rabdomiólise/complicações , Animais , Glicerol/administração & dosagem , Marcação In Situ das Extremidades Cortadas , Masculino , Ratos , Ratos Sprague-Dawley , Reação em Cadeia da Polimerase em Tempo Real
3.
Arch Gynecol Obstet ; 296(2): 205-213, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28589478

RESUMO

PURPOSE: To measure levels of placental brain derived neurotrophic factor (BDNF) gene expression and umbilical cord blood BDNF in neonates with nondiabetic macrosomia and determine associations between these levels and macrosomia. METHODS: This case-control study included 58 nondiabetic macrosomic and 59 normal birth weight mother-infant pairs. Data were collected from interviews and our hospital's database. BDNF gene expression was quantified in placental tissues using quantitative real-time polymerase chain reaction (n = 117). Umbilical cord blood BDNF levels were measured by enzyme-linked immunosorbent assay (n = 90). Multivariate logistic regression models were used to evaluate associations between BDNF levels and macrosomia. RESULTS: Placental BDNF gene expression (P = 0.026) and cord blood BDNF (P = 0.008) were lower in neonates with nondiabetic macrosomia than in normal birth weight controls. Cord blood BDNF was significantly lower in vaginally delivered macrosomic neonates than vaginally delivered controls (P = 0.014), but cord BDNF did not differ between vaginal and cesarean section delivery modes in macrosomic neonates. Cord blood BDNF was positively associated with gestational age in control neonates (r = 0.496, P < 0.001), but not in macrosomic neonates. Cord blood BDNF was positively associated with placental BDNF relative expression (r s = 0.245, P = 0.02) in the total group. Higher cord blood BDNF levels were independently associated with protection against nondiabetic macrosomia (adjusted odds ratio 0.992; 95% confidence interval 0.986-0.998). CONCLUSIONS: Both placental BDNF gene expression and cord blood BDNF were downregulated in neonates with nondiabetic macrosomia compared with normal birth weight neonates. Cord BDNF may partly derive from BDNF secreted by the placenta. Higher cord plasma BDNF levels protected against nondiabetic macrosomia.


Assuntos
Fator Neurotrófico Derivado do Encéfalo/metabolismo , Sangue Fetal/metabolismo , Macrossomia Fetal/sangue , Placenta/metabolismo , Adulto , Animais , Peso ao Nascer , Peso Corporal , Fator Neurotrófico Derivado do Encéfalo/genética , Estudos de Casos e Controles , Ensaio de Imunoadsorção Enzimática , Feminino , Macrossomia Fetal/genética , Regulação da Expressão Gênica , Idade Gestacional , Humanos , Recém-Nascido , Gravidez , RNA Mensageiro , Reação em Cadeia da Polimerase em Tempo Real
4.
PLoS One ; 11(3): e0151158, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26987113

RESUMO

Acute kidney injury (AKI) is one of the most severe complications of rhabdomyolysis (RM). The underlying mechanisms and potential preventions need to be investigated. Penehyclidine hydrochloride (PHC) was reported to ameliorate renal ischemia-reperfusion injury, but the effect of PHC on RM-reduced AKI is unknown. In this study, we established a rat model of RM-induced AKI using an intramuscular glycerol injection in the hind limbs. Rats were pretreated with PHC before the glycerol injection, and the heme oxygenase-1 (HO-1) inhibitor ZnPP was introduced to evaluate the effect of HO-1 on RM-induced AKI. PHC pretreatment ameliorated the pathological renal injury and renal dysfunction, and decreased the renal apoptosis rate in RM-induced AKI. PHC significantly up-regulated HO-1 expression, increased HO-1 enzymatic activity and decreased the accumulation of myoglobin in renal tissues. This effect was partly inhibited by ZnPP. PHC pretreatment also effectively up-regulated nuclear factor erythroid 2-related factor 2 (Nrf2) and down-regulated glucose regulated protein 78 (GRP78) and caspase-12 at both the gene and protein levels. These results suggest that the protective effects of PHC pretreatment on RM-induced AKI occur at least in part through activating the Nrf2/HO-1 pathway and alleviating endoplasmic reticulum stress (ERS) in rat renal tissues.


Assuntos
Lesão Renal Aguda/tratamento farmacológico , Lesão Renal Aguda/etiologia , Antagonistas Colinérgicos/uso terapêutico , Estresse do Retículo Endoplasmático/efeitos dos fármacos , Rim/efeitos dos fármacos , Quinuclidinas/uso terapêutico , Rabdomiólise/complicações , Lesão Renal Aguda/metabolismo , Lesão Renal Aguda/patologia , Animais , Heme Oxigenase-1/metabolismo , Rim/metabolismo , Rim/patologia , Masculino , Fator 2 Relacionado a NF-E2/metabolismo , Ratos , Ratos Sprague-Dawley , Transdução de Sinais/efeitos dos fármacos
5.
Mol Med Rep ; 13(4): 3273-80, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26934915

RESUMO

Macrosomia, a birth weight ≥ 4,000 g, is associated with maternal and infant health problems. The dysregulation of microRNAs (miRNAs) in the placenta is associated with adverse birth outcomes, yet whether aberrantly expressed placental miRNAs are associated with macrosomia remains unknown. The aim of the current study was to characterize the expression of three placental miRNAs (miR­6, ­21 and ­143) and evaluate their association with macrosomia. The miRNA expression in placental tissues from 67 macrosomic pregnancies and 64 normal pregnancies were analyzed using reverse transcription­quantitative polymerase chain reaction. The expression of miR­21 was observed to be elevated in macrosomic placenta compared with control samples, while miR­143 expression was significantly lower than in control placenta (P<0.05). No significant differences were identified in the miR­16 expression levels between the groups (P=0.955). Following division of miRNA expression levels by quartile, logistic regression models demonstrated that the odds of macrosomia increased with miR­21 expression quartile: Q2, odds ratio (OR)=6.67 [95% confidence interval (CI), 1.39­32.05]; Q3, OR=4.10 (95% CI, 0.88­19.11); Q4, OR=16.19 (95% CI, 2.46­106.68). Conversely, higher levels of miR­143 expression were protective against macrosomia: Q2, OR=0.22 (95% CI, 0.049­0.98); Q3, OR=0.11 (95% CI, 0.024­0.55), and Q4, OR=0.16 (95% CI, 0.032­0.79). Thus, statistical analysis demonstrated that high levels of miR­21 expression and low levels of miR­143 expression predict the risk for macrosomia, indicating an interaction between the two miRNAs. Bioinformatic analysis suggested that they are likely to function in the mitogen­activated protein kinases signaling pathway to influence the risk of macrosomia. The results of the present study provide evidence that placental miR-21 and -143 are important in the formation of macrosomia.


Assuntos
Macrossomia Fetal/patologia , MicroRNAs/metabolismo , Placenta/metabolismo , Adolescente , Adulto , Peso ao Nascer , Feminino , Macrossomia Fetal/genética , Humanos , Masculino , Razão de Chances , Gravidez , Reação em Cadeia da Polimerase em Tempo Real , Adulto Jovem
6.
J Matern Fetal Neonatal Med ; 29(1): 106-11, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-25394615

RESUMO

OBJECTIVE: To explore the relationship between birth weight and fat mass- and obesity-associated (FTO) gene expression and promoter methylation status in the Chinese population. METHODS: Seventy-five neonates and their mothers were recruited from Yuying Children's Hospital of Wenzhou Medical University. Subjects were divided into three groups by birth weight: low (< 3,500 g, n = 20), medium (3,500-3,999 g, n = 30) and high (≥ 4,000 g, n = 25). Placental FTO transcript levels and promoter methylation were determined by quantitative PCR and Sequenom MassARRAY®. RESULTS: Placental FTO mRNA expression was significantly increased in the high- and medium-weight groups compared to the low-weight group (p = 0.023). Methylation rates of CpG11 sites were significantly decreased in high-birth weight newborns (p = 0.018). Multiple linear regressions showed placental FTO mRNA, maternal pre-pregnancy body mass index (BMI) and CpG11 methylation rate were independently associated with increased fetal birth weight. Additionally, FTO mRNA expression was negatively associated with CpG6.7.8.9 methylation in mothers that underwent C-section. CONCLUSIONS: High placental FTO expression is associated with increased birth weight in Chinese neonates, and FTO promoter methylation level at a specific CpG site is negatively associated with birth weight. Further work is needed to determine the functionality of this CpG site in placentas.


Assuntos
Peso ao Nascer , Proteínas/genética , Adulto , Dioxigenase FTO Dependente de alfa-Cetoglutarato , Grupo com Ancestrais do Continente Asiático/genética , Cesárea , China , Ilhas de CpG , Metilação de DNA , Feminino , Expressão Gênica , Humanos , Recém-Nascido , Gravidez , Regiões Promotoras Genéticas , Estudos Prospectivos , Adulto Jovem
7.
Artigo em Chinês | MEDLINE | ID: mdl-23256995

RESUMO

OBJECTIVE: To investigate the effect of lead exposure on the gene expression of fibroblast growth factor 3 (Fgf3) in zebrafish embryonic development and the mechanism of lead-induced embryonic developmental toxicity. METHODS: The embryos of zebrafish (wild types A and B) were exposed to lead acetate (PbAc) at the doses of 0, 0.1, 0.5, 2.5, and 12.5 µmol/L separately. Total RNA was extracted from each treatment group of zebrafish embryos at 8, 12, 16, 24, 36, 48, and 72 hours post fertilization (hpf). The total mRNA expression of Fgf3 was measured by real-time quantitative PCR. The spatial expression of Fgf3 in zebrafish embryos was determined by whole-mount in situ hybridization using synthesized Fgf3 RNA probe. RESULTS: The mRNA expression of Fgf3 in each group peaked at 12 hpf (P < 0.01). With the increase in PbAc concentration, the mRNA expression of Fgf3 rose. Compared with the mRNA expression level of Fgf3 in the control group, the relative mRNA expression levels of Fgf3 in the 0.1, 0.5, 2.5, and 12.5 µmol/L PbAc exposure groups were 1.02 ± 0.24, 1.05 ± 0.26, 1.22 ± 0.46, and 1.25 ± 0.38, respectively, and the 2.5 and 12.5 µmol/L PbAc exposure groups showed significantly higher Fgf3 expression than the control group (P < 0.05). The whole-mount in situ hybridization results showed that Fgf3 expression occurred mainly in the head and tail in the early stage of embryonic development and in the midbrain, fin bud, and pharyngeal arch in the middle/late stage of embryonic development; there were the most significant regions and intensities of positive hybridization signals at 12 hpf; but no significant differences were found between the control group and exposure groups in the location and intensity of Fgf3 expression CONCLUSION: Lead exposure can result in the upregulation of Fgf3 expression in zebrafish embryonic development, which might contribute to lead-induced embryonic developmental toxicity.


Assuntos
Desenvolvimento Embrionário/efeitos dos fármacos , Fator 3 de Crescimento de Fibroblastos/genética , Compostos Organometálicos/efeitos adversos , Proteínas de Peixe-Zebra/genética , Peixe-Zebra/genética , Animais , Fator 3 de Crescimento de Fibroblastos/metabolismo , Expressão Gênica , Transdução de Sinais , Peixe-Zebra/embriologia , Peixe-Zebra/metabolismo , Proteínas de Peixe-Zebra/metabolismo
8.
J Hum Genet ; 57(6): 375-8, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22534769

RESUMO

We previously performed a survey of the sperm characteristics of the partners of pregnant women in four cities in Japan. In the present study, we analyzed the sperm characteristics of these subjects and the correlations between these sperm characteristics and climatic changes or Y chromosome haplogroups. Our results showed that more haplogroup D2a1 males than O2b1 males were born in the first half of the year (January to June), whereas more O2b1 males were born in the last half of the year (July to December) (P<0.05). This was agreed and correlated with the seasonal variations in their mean sperm concentrations. The haplogroup C, D* and D2a1 males displayed lower sperm concentrations from March to May, followed by an increase in their sperm concentrations starting in June or July, while the O2b1 males displayed higher sperm concentrations in the first half of the year followed by a sudden decrease from July to August (P<0.05). We hypothesize that the Japanese climate has different effects on the sperm characteristics and reproductive seasonality of males from different lineages; and therefore, has influenced the modern population of Japan.


Assuntos
Grupo com Ancestrais do Continente Asiático/genética , Clima , Reprodução/genética , Adulto , Cromossomos Humanos Y , Interação Gene-Ambiente , Aptidão Genética , Marcadores Genéticos , Humanos , Japão , Masculino , Pessoa de Meia-Idade , Filogenia , Contagem de Espermatozoides , Adulto Jovem
9.
Artigo em Chinês | MEDLINE | ID: mdl-21619792

RESUMO

OBJECTIVE: To detect the expression and the CpG island methylation status of tumor suppressor gene p15 after exposure to 1,4-benzoquinone (1,4-BQ) in primary cultivated C57BL/6J mouse bone marrow cells in vitro. METHODS: The mouse bone marrow cells were isolated in vitro. The effect of 0, 0.1, 1, 5, 10, 20, and 40 µmol/L 1,4-BQ on cell viability (CKK-8) was detected. 0, 0.1, 1, 10 µmol/L 1,4-BQ were used to intoxicate the mouse bone marrow cells for 24 h; Real-time PCR was employed to analyze the mRNA expression level of p15; The bisulfite sequencing PCR (BSP) was used to look into the methylation status of CpG islands in p15 promoter region. RESULTS: 1,4-BQ exhibited dose-dependent toxicity to mouse bone marrow cells, and the LC(50) was 8.3 µmol/L (95%CI: 4.6 - 10.6 µmol/L). The mRNA expression of p15 in 10 µmol/L group was only equivalent to 43% of control group. Compared with control group, the decrease of p15 mRNA expression in1 and 10 µmol/L concentration were obvious, and the differences had statistical significance (P < 0.05 or P < 0.01). BSP sequencing results were same between the exposure groups and control group, the 56 CpG sites on CpG islands remained in the state of unmethylated. CONCLUSION: mRNA expression of p15 gene decreases after exposure to 1,4-BQ, but the CpG islands methylation status in promoter is not affected, suggesting that methylation does not participate in 1,4-BQ-mediated p15 gene expression decrease, other effect mechanisms still need to be investigated.


Assuntos
Benzoquinonas/toxicidade , Células da Medula Óssea/metabolismo , Inibidor de Quinase Dependente de Ciclina p15/genética , Metilação de DNA , Exposição Ambiental , Animais , Sequência de Bases , Células Cultivadas , Ilhas de CpG , Camundongos , Camundongos Endogâmicos C57BL , Regiões Promotoras Genéticas , RNA Mensageiro/genética
10.
Eur J Ophthalmol ; 21(4): 355-62, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21240858

RESUMO

PURPOSE: To evaluate the long-term effects of photorefractive keratectomy (PRK) correction for myopia in China. METHODS: A comprehensive literature research was conducted in 2 Chinese databases and PubMed. Statistical analysis was performed using the RevMan 4.2 software. RESULTS: Seven prospective studies involving 2,796 eyes were included. The follow-up period was 5 to 11 years. Meta-analysis showed efficacies in low to moderate myopia that surpassed those in high myopia, and pooled odds ratios were 9.14 (95% confidence interval [CI] 4.37-19.11) in the proportion of eyes with uncorrected visual acuity (UCVA)=20/20 and 5.61 (95% CI 3.97-7.93) in the proportion of eyes with UCVA=20/40. More than 10 years post operation, 82.0% had 20/20 or better UCVA and 96.1% had 20/40 or better in the =-6.00 D myopia group. Among patients with >-6.00 D myopia, 31.7% had 20/20 or better UCVA and 80.4% had 20/40 or better. Moreover, 82.1% and 46.4% of the treated eyes were within ±1.0 D of target refraction in the low to moderate myopia and high myopia group, respectively. About 1.4%-3.5% of eyes lost =2 lines of best spectacle-corrected visual acuity, and 0.6%-4.1% of eyes had grade 1 corneal haze. CONCLUSIONS: Photorefractive keratectomy for myopia is an effective and safe procedure for the long term in China. The efficacy and predictability of PRK in low to moderate myopia are better than in high myopia.


Assuntos
Lasers de Excimer/uso terapêutico , Miopia/cirurgia , Ceratectomia Fotorrefrativa , China/epidemiologia , Bases de Dados Factuais , Seguimentos , Humanos , Miopia/epidemiologia , Miopia/fisiopatologia , Resultado do Tratamento , Acuidade Visual/fisiologia
11.
Zhonghua Yi Xue Za Zhi ; 90(7): 439-42, 2010 Feb 23.
Artigo em Chinês | MEDLINE | ID: mdl-20368063

RESUMO

OBJECTIVE: To understand and address our vision changes in primary and secondary students, in order to explore the prevention of juvenile myopia to provide a response and suggestions. METHODS: A random cluster sampling method, random sample of 90 rural and urban schools in Shandong, Qinghai, Yunnan, Shanghai and Wenzhou from May 2008 to September 2009, including 20 000 students (per province, 4000 x 5) from elementary school and junior high school of focus and non-focus, primary 11 246, junior high school students 3673, high school 4220, of which 11 177 were from the urban students, rural students in 7962, aged 6 to 19 years old, male to female ratio is basically the same. For myopia and related survey, a total of 19, 139 valid questionnaires were recovered. Using SPSS 13.0 software statistical analysis, different regions and different grades was used to compare the prevalence of myopia chi(2) test, using Logistic regression analysis on the major risk factors for myopia. RESULTS: The survey are as follows, the prevalence of myopia in primary was 13.7%; the prevalence of myopia in junior high was 42.9%, the prevalence of myopia in high school was 69.7%. The highest rate of myopia prevalence of primary school students in Shandong was 19.7%, the lowest was 11.9% in Qinghai, Shanghai, Wenzhou and Yunnan are similar; prevalence myopia of junior high in Wenzhou up to 50.6%, followed by Shanghai, and 47.2%, the lowest was 33.6% in Qinghai; the highest prevalence of myopia and high school students in Shandong, reaching 87.3%, followed by 72.0% in Wenzhou, and the lowest was 60.2% in Shanghai. CONCLUSION: This survey shows that the prevalence of myopia of China's primary and secondary schools in coastal was higher than areas of mainland, in developed regions was higher than in less developed regions, and the plateau region and the strong ultraviolet light similar to the plain areas. Provincial prevalence of myopia increased from primary to high school continued the same trend.


Assuntos
Miopia/epidemiologia , Adolescente , Criança , China/epidemiologia , Feminino , Humanos , Masculino , Prevalência , Fatores de Risco , Estudantes , Adulto Jovem
12.
J Glaucoma ; 19(1): 19-23, 2010 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-20075673

RESUMO

PURPOSE: To propose a new surgical technique for optimized visualization of the chamber angle using ophthalmic microendoscope in goniosynechialysis (GSL). METHODS: Patients who had acute angle-closure glaucoma with peripheral anterior synechiae or patients with flat anterior chamber after trabeculectomy underwent endoscopically controlled GSL. Ophthalmic endoscope was used before, during, and immediately after GSL to minimize the procedure of GSL and to ensure that the trabecular meshwork was exposed and the majority of the angle was opened after endoscopically controlled GSL. Intraoperative complications, postoperative visual acuity, intraocular pressures (IOPs), and complications were all evaluated. RESULTS: Twelve eyes of twelve patients underwent the operation and the mean follow-up was 7.4+/-1.4 months (range: 6 to 10 mo). The mean preoperative IOP was 42.89+/-15.81 mm Hg. The mean postoperative IOP at the most recent follow-up was 12.72+/-3.48 mm Hg. The absolute success rate (IOP <21 mm Hg without medication) was 8 of 10. Visual acuity improved in 11 of 12 patients (91.7%). No significant intraoperative complications, such as iridodialysis, occurred in any patient. Postoperative complications included hyphema and transient corneal decompensation. CONCLUSIONS: Endoscope conveniently provided the surgeon an optimized visualization of the anterior chamber angle. This enhanced visualization and convenience promises accuracy and safety when performing GSL.


Assuntos
Câmara Anterior/cirurgia , Corpo Ciliar/cirurgia , Endoscopia , Glaucoma de Ângulo Fechado/cirurgia , Malha Trabecular/cirurgia , Doença Aguda , Adulto , Idoso , Feminino , Seguimentos , Gonioscopia , Humanos , Pressão Intraocular/fisiologia , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias , Estudos Prospectivos , Acuidade Visual/fisiologia
13.
Zhonghua Yi Xue Za Zhi ; 89(21): 1464-7, 2009 Jun 02.
Artigo em Chinês | MEDLINE | ID: mdl-19953897

RESUMO

OBJECTIVE: To evaluate the long-term trend of cataract prevalence and the effect of prevention and treatment blindness in Zhejiang Province. METHODS: The data were obtained from handicapped sampling investigation in 1987 and 2006 in Zhejiang Province, and from a database of prevention and treatment blindness of the Zhejiang Province Disabled Persons' Federation. RESULTS: In 2006, a random sampling investigation were carried out on both eyes blind and the low vision in Zhejiang Province. Sample size were 95,392, the prevalence rate of blindness and low vision were 2.67% per hundred and 5.94% per hundred, respectively. The prevalence rate of cataract was 7.35% per hundred, the female was higher than the male (chi2 = 81.66, P < 0.01). Among the blind, 58.86% was caused by cataract, and the low vision caused by cataract was 63.79%. Comparing with the first disabled person' investigation in 1987, the result showed that the prevalence of blindness had reduces slightly, but no significantly difference (chi2 = 0.384, P = 0.535), and that the prevalence of low vision appeared significantly increase (chi2 = 32.434, P < 0.01). Together with two investigations results indicated that cataract is the first cause for the vision disability. CONCLUSION: Cataract is still the first cause for blindness and low vision. Though the effort to prevention and treatment blindness during 20 years obtains some certain results, prevent and treat blindness will still be an arduous work in Zhejiang province, because of accelerated aging population problem, which will induce an increase on number of cataract patient.


Assuntos
Catarata/epidemiologia , Idoso , Cegueira/prevenção & controle , China/epidemiologia , Feminino , Humanos , Masculino , Prevalência , Baixa Visão/prevenção & controle
14.
Ying Yong Sheng Tai Xue Bao ; 18(4): 937-40, 2007 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-17615897

RESUMO

The virulence test of five species of entomogenous fungi Beauveria brongniartii, Beauveria bassiana, Paecilomyces fumosoroseus, Metarhizium anisopliae and Nomuraea rileyi to Spodoptera litura larvae showed that B. brongniartii and N. rileyi had evident pathogenic effects on S. litura, with the LT50 value to S. litura 2nd instars being 2.95 and 4.10 days, and the corrected accumulative mortality of the instars being 100% and 95.2%, respectively. The virulence of B. brongniartii and N. rileyi to the 3rd instars was lower than that of 2nd instars. The LT50 value to 3rd instars was 19.67 and 19.63 days, and the corrected accumulative mortality was 56.6% and 52.2%, respectively. Other two fungal species P. fumosoroseus and B. bassiana also had virulence to S. litura larvae. The LT50 value for the 2nd instars was 4.89 and 6.34 days, and the corrected accumulative mortality reached 85.7% and 71.4%, respectively.


Assuntos
Beauveria/patogenicidade , Fungos/patogenicidade , Controle Biológico de Vetores/métodos , Spodoptera/microbiologia , Animais , Beauveria/fisiologia , Fungos/fisiologia , Larva/microbiologia , Paecilomyces/patogenicidade , Paecilomyces/fisiologia
15.
Mol Cells ; 24(3): 323-8, 2007 Dec 31.
Artigo em Inglês | MEDLINE | ID: mdl-18182846

RESUMO

SOX (Sry-related HMG box) family proteins, which have an evolutionarily conserved DNA binding domain, have crucial roles in cell differentiation. However, their target genes remain enigmatic. Some members of the SOX family may have roles in regulation of cell proliferation. We established stable NT2/D1 cell lines overexpressing SOX15 (SOX15-NT2/D1), and a modified 3-(4,5-dime-thylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the SOX15-NT2/D1 cells exhibited significantly slower growth than the controls. Flow cytometry analysis revealed that an increased fraction of the SOX15-NT2/D1 cells were in G1-G0. In addition, a microarray analysis identified 26 genes that were up-regulated in the SOX15-NT2/D1 cells, but none that were down-regulated genes. Among the up-regulated genes, IGFBP5, S100A4, ID2, FABP5, MTSS1, PDCD4 have been shown to be related to cell proliferation and/or the cell cycle.


Assuntos
Proliferação de Células/efeitos dos fármacos , Proteínas de Ligação a DNA/biossíntese , Proteínas de Grupo de Alta Mobilidade/biossíntese , Carcinoma Embrionário/patologia , Linhagem Celular Tumoral , Citometria de Fluxo , Expressão Gênica , Humanos , Masculino , Análise de Sequência com Séries de Oligonucleotídeos , RNA Mensageiro/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fatores de Transcrição SOX , Neoplasias Testiculares/patologia
16.
J Med Invest ; 53(1-2): 147-52, 2006 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-16538008

RESUMO

Around 10% of males with idiopathic azoospermia or oligozoospermia, which are important causes of male infertility, have partial deletions on the long arm of the Y chromosome. To develop a rapid and accurate detection system for screening major Y deletions found in infertile men, we developed a multiplex PCR system that can simultaneously amplify five loci on the Y chromosome, SRY, AMELY, DBY, RBMY, DAZ and one locus on the X chromosome, AMELX. The size of the PCR products was designed to increase gradually from the distal Yp to the distal Yq. Our system could detect deletions of three major candidate regions for the azoospermic factor, AZFa, AZFb and AZFc on the Y chromosome together with sex. The gradual increase in the size of the PCR products was convenient for imaging the location of deletions on the Y chromosome. Moreover, the multiplex PCR system was combined with microchip-based electrophoresis, and the PCR products derived from each locus were separated within 4 min. Our system is useful for screening Y chromosomes bearing the structural anomalies including three major AZF deletions found among azoospermic or oligozoospermic males.


Assuntos
Deleção Cromossômica , Cromossomos Humanos Y/genética , Oligospermia/genética , Reação em Cadeia da Polimerase/métodos , Sequência de Bases , Estudos de Casos e Controles , Primers do DNA/genética , Eletroforese/métodos , Loci Gênicos , Humanos , Masculino , Proteínas de Plasma Seminal/genética
17.
Mol Hum Reprod ; 11(7): 513-5, 2005 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-16123080

RESUMO

The DAZL (DAZ-like) gene is suggested to be an ancestral gene of the DAZ (deleted in azoospermia) gene on the Y chromosome, which is a strong candidate for the azoospermic factor. Recently, it has been reported that the T54A (Thr54-->Ala) polymorphism in exon 3 of the DAZL gene is associated with spermatogenic failure in the Taiwanese population. In this study, to investigate whether this polymorphism is associated with spermatogenic failure in Japanese males, we analysed genomic DNA derived from 234 patients with azoospermia or oligozoospermia and 131 fertile controls. The T54A polymorphism was completely absent in both the patients and the controls. The T12A (Thr12-->Ala) polymorphism in exon 2 of the DAZL gene was found at a similar frequency in the patients and controls, 15.4% and 13.7%, respectively (P = 0.67). However, the frequency of T12A was higher for the azoospermic (20.5%) than oligozoospermic (9.6%) individuals in infertile men without DAZ deletions, although statistical difference was not so apparent (chi2 test: P = 0.037, OR = 2.413, 95% CI = 1.035-5.629; Yate's chi2 test: P = 0.058, OR = 2.319, 95% CI = 0.973-6.166). Our results show that the T54A polymorphism in DAZL has no major role in Japanese males with azoospermia or oligozoospermia. The distribution of the T54A polymorphism may be restricted to the narrow area including Taiwan.


Assuntos
Infertilidade Masculina/genética , Oligospermia/genética , Polimorfismo Genético , Proteínas de Ligação a RNA/genética , Adulto , Humanos , Japão , Masculino , Pessoa de Meia-Idade , Mutação Puntual , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição
18.
J Hum Genet ; 50(4): 175-81, 2005.
Artigo em Inglês | MEDLINE | ID: mdl-15834507

RESUMO

Recent progress in sequencing the human Y chromosome has unveiled a series of X-Y homologous genes. In the present study, we focused on Transducin beta-like 1Y (TBL1Y), which is a Y-linked homologue of TBL1X that is related with X-linked late-onset sensorineural deafness. Recently, it has been shown that TBLR1, another homologue whose gene resides on chromosome 3, and TBL1X act as a corepressor/coactivator exchanger for several nuclear receptors and transcription factors. However, the expression pattern and function of TBL1Y remain unknown. The RT-PCR analysis of the TBL1 family revealed that TBL1Y was expressed in all 13 tissues examined but not in leukocytes. Among the cell lines tested, however, it was only expressed in NT2/D1 cells and in lymphoblasts transformed with Epstein Barr (EB) virus. To compare the functions of the TBL1 family, we generated a series of expression plasmids for GAL4DBD-fused proteins of the TBL1 family. We carried out dual luciferase assays using these plasmids in combination with a plasmid having a luciferase reporter gene harboring 5xGAL4 binding sites. Unlike the other constructs, GAL4DBD-fused TBL1Y did not repress the promoter activity. Moreover, we found three novel polymorphisms in the TBL1Y gene, IVS7+9G>A, G268C, and IVS7+1G>C, which is presumed to cause splicing error. These polymorphisms are found in males within Y-haplogroup O3 (XO3e), which is defined as the Y-haplogroup O3 excluding O3e, a branch of O3. The results show that TBL1Y differs from other members of the TBL1 family in expression and function, suggesting other roles in maleness.


Assuntos
Cromossomos Humanos Y/genética , Ligação Genética , Perda Auditiva Neurossensorial/genética , Transducina/genética , Idade de Início , Sequência de Aminoácidos , Cromossomos Humanos X/genética , Feminino , Humanos , Luciferases/metabolismo , Masculino , Dados de Sequência Molecular , Polimorfismo Genético , Regiões Promotoras Genéticas/genética , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , Homologia de Sequência de Aminoácidos
19.
J Med Invest ; 50(3-4): 180-6, 2003 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-13678388

RESUMO

OBJECTIVE: To evaluate the effectiveness of laser in situ keratomileusis (LASIK) and photorefractive keratectomy (PRK) for correcting myopia. METHODS: Study selection, data extraction, and quality assessment were performed by two of authors independently. Summary odds ratios and 95% confidence intervals were calculated by DerSimonian & Laird random-effects model and Mantel-Haenszel (fixed-effects) model. All calculations were based on an intention-to-treat and per protocol analysis. RESULTS: Five hundred and eighty eyes (476 patients) from 5 randomized controlled trials were included in this study. At > or = 6 months follow-up, by random-effects model, the pooled odds ratios (OR, for LASIK vs. PRK) of postoperative uncorrected visual acuity (UCVA) of 20/20 or better for all trials were 1.31 (95% CI=0.77-2.22) by per protocol analysis and 1.18 (95% CI=0.74-1.88) by intention-to-treat analysis. In the refractive outcome, the pooled OR of the postoperative spherical equivalent refraction within +/-0.5 diopter (D) of emmetropia did not show any statistical significance, for which the OR were 0.75 (95% CI=0.48-1.18) by per protocol analysis and 0.70 (95% CI=0.47-1.04) by intention-to-treat analysis. CONCLUSIONS: LASIK and PRK were found to be similarly effective for the correction of myopia from -1.5 to -15.0 D in a greater than 6 month follow-up.


Assuntos
Ceratomileuse Assistida por Excimer Laser In Situ , Miopia/cirurgia , Ceratectomia Fotorrefrativa , Humanos , Lasers de Excimer , Ensaios Clínicos Controlados Aleatórios como Assunto , Resultado do Tratamento
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