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1.
Leg Med (Tokyo) ; 53: 101957, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34481193

RESUMO

The Microreader™ 19X Direct ID System was a newly developed multiplex PCR kit, which could detect 19 X-chromosomal STR loci (DXS6795, DXS9907, DXS6803, GATA172D05, DXS6807, GATA31E08, DXS7423, DXS6810, DXS101, DXS9902, DXS7133, DXS6800, DXS981, DXS10162, DXS6809, DXS10135, HPRTB, GATA165B12, DXS10079) and the sex determination locus of AMEL simultaneously. Different from other X-STR multiplex PCR kits, no linkage groups are included in this system, so the likelihood ratios could be calculated without the consideration of linkage groups. In this study, PCR conditions, sensitivity, species specificity, stability, DNA mixtures, concordance, stutter, sizing precision and population studies were conducted according to the SWGDAM developmental validation guidelines. The results indicated that this new X-STRs multiplex system was an efficient and reliable detection system, which could facilitate human kinship analysis and identification testing, as a powerful supplementary to autosomal STR kits.

2.
Database (Oxford) ; 20212021 06 22.
Artigo em Inglês | MEDLINE | ID: mdl-34156445

RESUMO

Producing findable, accessible, interoperable and reusable (FAIR) data cannot be accomplished solely by data curators in all disciplines. In biology, we have shown that phenotypic data curation is not only costly, but it is burdened with inter-curator variation. We intend to propose a software platform that would enable all data producers, including authors of scientific publications, to produce ontologized data at the time of publication. Working toward this goal, we need to identify ontology construction methods that are preferred by end users. Here, we employ two usability studies to evaluate effectiveness, efficiency and user satisfaction with a set of four methods that allow an end user to add terms and their relations to an ontology. Thirty-three participants took part in a controlled experiment where they evaluated the four methods (Quick Form, Wizard, WebProtégé and Wikidata) after watching demonstration videos and completing a hands-on task. Another think-aloud study was conducted with three professional botanists. The efficiency effectiveness and user confidence in the methods are clearly revealed through statistical and content analyses of participants' comments. Quick Form, Wizard and WebProtégé offer distinct strengths that would benefit our author-driven FAIR data generation system. Features preferred by the participants will guide the design of future iterations.

3.
Int J Legal Med ; 135(3): 755-760, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-32468171

RESUMO

In 2009, a violent murder occurred. Two victims, a 47-year-old mother and her 21-year-old daughter, were murdered at home. After importing the 20 autosomal STR loci and 27 Y-STR loci into a database, no hit had been found. In 2019, a person with a prior criminal record was matched in the national forensic Y-STR database. When increasing the number of detected Y-STR loci to 60, all loci of the bloodstain donor at the crime and the suspect were still found to be identical. With the combined calculation of multiple autosomal STR and kinship index, we were able to identify the perpetrator as a previously unknown illegitimate child of a large family and solved the case.


Assuntos
Manchas de Sangue , Cromossomos Humanos Y/genética , Impressões Digitais de DNA/métodos , Genética Forense , Loci Gênicos , Homicídio , Repetições de Microssatélites , Adoção , Família , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fatores de Tempo , Adulto Jovem
4.
Am J Transl Res ; 12(7): 3429-3444, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32774710

RESUMO

Curcumin is a safe, cost-effective natural agent with multiple targets that displays therapeutic potential in cancer. Recently, we reported a novel curcumin analog, Da0324, which exhibited significantly improved stability and anti-cancer activity. However, the molecular mechanism underlying the anti-cancer activity of Da0324 remains largely unknown. Long non-coding RNAs have been shown to play important roles in cancer development and progression and may be potential targets for cancer therapy. Here, we showed that Da0324 treatment down-regulated the expression of LINC01021 in gastric cancer cells. Da0324 treatment or knockdown of LINC01021 by antisense oligos significantly inhibited gastric cancer cell growth, and also up-regulated P53 expression and down-regulated Bcl-2 expression in vitro and in vivo. Furthermore, Da0324 treatment or knockdown of LINC01021 in gastric cancer cells suppressed cell migration, invasion and epithelial-mesenchymal transition (EMT), as well as induced apoptosis and autophagy. In addition, overexpression of LINC01021 promoted growth and EMT, inhibited P53 expression and increased Bcl-2 expression in gastric cancer cells. Finally, overexpression of LINC01021 reversed the anti-cancer effect of Da0324. Our findings indicate a novel anti-cancer mechanism for Da0324, and that LINC01021 might be a potential therapeutic target for the treatment of gastric cancer.

5.
Forensic Sci Int Synerg ; 2: 210-214, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32705079

RESUMO

There have been many cases of pneumonia caused by novel coronavirus infections in China and around the world. This will inevitably lead to a rise in the number of patients. At the present time, clinical and forensic autopsies have given guidance and explanations in relation to the problem of COVID-19 transmission and defense. However, less attention is paid to the handling of COVID-19 biological samples in forensic practice. Particularly, COVID-19 can survive on some surfaces for days. Since there were many cases involving COVID-19 during the epidemic, this article shares the methods and strategies for handling such inspection materials and the biological samples related specifically to COVID-19 cases.

6.
Forensic Sci Int ; 314: 110370, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32682216

RESUMO

A case study involving an intentional homicide case in November 2018, in which the autosomal genotypes of the suspect were unavailable and only part of deletions of Y-STR loci were identified by Y-chromosomal typing. The suspect, male, was charged with beating the decedent, female, over the head with an iron water pipe to death. The use of standard autosomal DNA profiling to identify the suspect was unattainable due to the extensive volume blood of the decedent on the murder weapon which was inevitably cleaned by running water at the crime scene. As a result, autosomal genotypes of the suspect were unavailable and only partial samples of deletions of Y-STR loci were identified by Y-chromosomal typing. Y-STR analysis (Yfiler™ plus and AGCU Y36) was used on the collected DNA extracts and compared to reference samples of the suspect, as well as his father and brother in an attempt to positively identify the suspect as the perpetrator of the murder. Subsequent Y-STR genotyping for the suspect, his father and brother indicated that Y-STR genotype of the suspect was consistent with that discovered on the physical evidence and the deleted Y-STR loci were identical for both. No deletions of Y-STR genotype were observed in the suspect's father and brother. After changing a Y-STR kit, the deleted loci were still present in the suspect. In Addition, sequencing of the whole Y-chromosomal genes was performed on the samples taken from the suspect and his father and brother. Segmental deletions at Yq 11.222-Yq 11.23 of the suspect were observed and the deleted Y-STR markers were right on the deleted Y-chromosomal segments. In this case, although the suspect could not be identified by the autosomal STR profiles detected on the physical evidence, the discovery of identical Y-STR genotype and the identical deletions of Y-chromosomal segments made it plausible that DNA on the murder weapon was left behind by the suspect. This case study shows that in criminal cases like this, where the autosomal STR evidence is unattainable, Y-STR evidence can be used effectively as a substitute to identify the suspect.


Assuntos
Deleção Cromossômica , Cromossomos Humanos Y , Homicídio , Impressões Digitais de DNA , Feminino , Haplótipos , Humanos , Masculino , Repetições de Microssatélites , Reação em Cadeia da Polimerase
7.
J Gastrointestin Liver Dis ; 29(1): 51-58, 2020 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-32176759

RESUMO

BACKGROUND AND AIMS: Studies on the association between homocysteine and non-alcoholic fatty liver disease (NAFLD) have shown inconsistent results. Our study concerns the association of homocysteine with the histological severity of NAFLD, especially non-alcoholic steatohepatitis (NASH) and significant fibrosis (SF) after adjusting for other well-identified risk factors. METHODS: This study enrolled 289 patients with biopsy proven NAFLD. The association of homocysteine with the severe histological features was examined using multivariable logistic regression analysis and subgroup analysis. The area under curves (AUC) and Hosmer-Lemeshow goodness-of-fit test for the adjusted logistic regression models was analyzed. RESULTS: After multivariable regression analysis, homocysteine showed significant correlation with NASH (OR 0.79 95%CI: 0.69-0.89), p<0.001) and SF (OR 0.83 95%CI: 0.72-0.95, p=0.009). Spearman's correlation analysis showed homocysteine levels were inversely correlated with the grade of hepatocellular ballooning and the stage of liver fibrosis (Spearman's ρ=-0.13, p=0.033; Spearman's ρ=-0.16, p=0.007), but had no correlation with the severity of steatosis and lobular inflammation. The subgroup analyses showed that homocysteine was strongly associated with NASH in females but was weaker in males (female OR: 0.61 95%CI: 0.45-0.84; male 0.86 95%CI: 0.75-0.99), and on SF showed no significant differences in the subgroups. The models showed good discrimination for NASH (AUC 0.789, 95% CI: 0.736-0.843) and for SF (0.784 95%CI: 0.719-0.848) and calibration (Hosmer-Lemeshow goodness-of-fit test, p=0.346 for NASH; p=0.908 for SF). CONCLUSION: Elevated serum homocysteine levels are negatively associated with NASH and SF in subjects with NAFLD.


Assuntos
Homocisteína/sangue , Cirrose Hepática , Fígado/patologia , Hepatopatia Gordurosa não Alcoólica , Biomarcadores/sangue , Biópsia/métodos , Biópsia/estatística & dados numéricos , China , Correlação de Dados , Estudos Transversais , Feminino , Humanos , Cirrose Hepática/sangue , Cirrose Hepática/patologia , Masculino , Pessoa de Meia-Idade , Hepatopatia Gordurosa não Alcoólica/sangue , Hepatopatia Gordurosa não Alcoólica/epidemiologia , Hepatopatia Gordurosa não Alcoólica/patologia , Fatores Sexuais
9.
Int J Legal Med ; 134(2): 501-503, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30675640

RESUMO

To investigate the genetic variation and forensic efficiency of 19 X-STR loci in the Zhuang and Mulao minorities, we calculated allele frequencies, forensic parameters, and haplotype frequencies of 405 Zhuang and 234 Mulao unrelated healthy individuals from Guangxi Zhuang Autonomous Region of China. Furthermore, we analyzed the population genetic differentiations between the two minorities and other reported Chinese populations.


Assuntos
Cromossomos Humanos X , Grupos Étnicos/genética , Variação Genética , Haplótipos , Repetições de Microssatélites , Grupo com Ancestrais do Continente Asiático/genética , China/etnologia , Feminino , Frequência do Gene , Genética Populacional , Humanos , Masculino
10.
Turk J Gastroenterol ; 30(10): 883-891, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31633484

RESUMO

BACKGROUND/AIMS: Metabolic acidosis is a common complication in patients with cirrhosis at the intensive care units (ICUs) and associated with increased mortality. The aim of our research was to explore the epidemiology and risk factors of metabolic acidosis in critically ill patients with cirrhosis. MATERIALS AND METHODS: A total of 975 patients with cirrhosis were selected into our study, and all participants were followed up for at least 28 days. Cox regression model and machine-learning algorithm were used to identify the importance of different risk factors, respectively. Finally, an improved prognostic model as Model for End-stage Liver Disease and metabolic acidosis (MELD-MA) was developed. RESULTS: Among the 975 patients with liver cirrhosis, 506 had metabolic acidosis, including 257 patients who had decompensated metabolic acidosis at ICU admission. The 28-day mortality was 41% (206/506) in patients with metabolic acidosis. Bilirubin (hazard ratio (HR): 1.023, 95% confidence interval (CI): 1.011-1.036), international normalized ratio (HR: 1.527, 95% CI: 1.332-1.750), pH (HR: 0.173, 95% CI: 0.047-0.640), BE-Lac (HR: 0.907, 95% CI: 0.868-0.948), and BE-Na (HR: 0.923, 95% CI: 0.859-0.991) were considered as independent prognostic parameters for 28-day mortality. MELD-NA had significantly higher discrimination (area under the receiver operating characteristic curve 0.79) than MELD and Child-Pugh score. CONCLUSION: Critically ill patients with cirrhosis have a high mortality rate and poor prognosis because of the high prevalence of metabolic acidosis. Lactic acidosis is the worst prognosis of all types of metabolic acidosis. MELD-MA performs well on the short-term mortality assessment in critically ill patients with cirrhosis and metabolic acidosis.


Assuntos
Acidose/mortalidade , Cirrose Hepática/mortalidade , Acidose/etiologia , Idoso , Estado Terminal/mortalidade , Feminino , Humanos , Unidades de Terapia Intensiva/estatística & dados numéricos , Cirrose Hepática/complicações , Masculino , Pessoa de Meia-Idade , Prognóstico , Modelos de Riscos Proporcionais , Fatores de Risco , Índice de Gravidade de Doença
11.
Biomed Pharmacother ; 117: 109126, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31387165

RESUMO

Approximately 40% of patients with non-small cell lung cancer (NSCLC) develop bone metastasis. The formin protein formin-like 1 (FMNL1) plays a key role in the pathogenic processes of hematopoietic malignancies, and has been reported to be associated with the progression of multiple types of cancer. In the study, we found that FMNL1 expression was markedly up-regulated in primary NSCLC samples, and stronger expression of FNML1 was detected in bone metastasis. Reducing FMNL1 expression significantly suppressed cell proliferation in NSCLC cells. We also investigated the functional effects of FMNL1 knockdown on the inhibition of migration and invasion by meditating the expression of epithelial to mesenchymal transition (EMT)-associated signals in NSCLC cells. The transforming growth factor-ß1 (TGF-ß1)/SMADs signaling pathway was repressed in FMNL1-knockdown NSCLC cells. Further studies indicated that additional treatment with TGF-ß1 could markedly abrogate FMNL1 knockdown-induced suppression of migration and invasion in NSCLC cells. In addition, NSCLC cell-induced osteoclastogenesis was also inhibited by FMNL1 deletion, as evidenced by the down-regulated expression of tartrate-resistant acid phosphatase (TRAP) and NFATc1. In vivo studies confirmed the results that FMNL1 knockdown markedly limited tumor growth. Importantly, decreasing FMNL1 reduced bone metastasis ability in vivo. Therefore, our results demonstrated that suppressing FMNL1 expression could inhibit bone metastasis in NSCLC through blocking TGF-ß1 signaling, and FMNL1 might be a novel target for developing effective therapeutic strategy to limit the bone metastasis of NSCLC.


Assuntos
Neoplasias Ósseas/genética , Carcinoma Pulmonar de Células não Pequenas/genética , Regulação para Baixo/genética , Forminas/genética , Neoplasias Pulmonares/genética , Metástase Neoplásica/genética , Fator de Crescimento Transformador beta1/genética , Células A549 , Animais , Neoplasias Ósseas/patologia , Carcinoma Pulmonar de Células não Pequenas/patologia , Linhagem Celular , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Transição Epitelial-Mesenquimal/genética , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Neoplasias Pulmonares/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Nus , Pessoa de Meia-Idade , Metástase Neoplásica/patologia , Transdução de Sinais/genética
12.
Int J Legal Med ; 133(6): 1691-1698, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31317316

RESUMO

From Southern Han Chinese samples, we analyzed 19 X-STR markers for linkage, linkage disequilibrium (LD), and mutation rate. The data were collected from two- and three-generation Southern Han Chinese families. These data suggested that both linkage and linkage disequilibrium should be considered while calculating likelihood ratios with X-STR markers in relationship tests. The linkage disequilibrium of these 19 X-STR markers was calculated in our previous research study that was conducted on Southern Han Chinese population. In this study, the recombination fractions between pairs of markers and those obtained from the second-generation Rutgers combined linkage-physical map of the human genome were compared. The observed differences indicated that recombination was not homogeneous along the X chromosome. Therefore, we evaluated the effect on likelihood calculations by referring to haplotype frequencies obtained from allele distributions rather than haplotype counts of Southern Han Chinese population.


Assuntos
Cromossomos Humanos X , Grupos Étnicos/genética , Ligação Genética , Repetições de Microssatélites , Taxa de Mutação , Recombinação Genética , Grupo com Ancestrais do Continente Asiático/genética , China , Feminino , Haplótipos , Humanos , Funções Verossimilhança , Desequilíbrio de Ligação , Masculino , Linhagem
13.
Iran J Public Health ; 48(3): 474-483, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31223575

RESUMO

Background: County public hospital reform is one of the major tasks proposed in Chinese Healthcare Reform., and the evaluation of hospital reform effectiveness is very important and beneficial since it helps the government to understand the current situation of pilot county public hospitals and smoothly start the reform in all county hospitals. Methods: This study used hospitals data from 2009 to 2012 to evaluate the effectiveness of county public hospital reform through comprehensive service capability. Descriptive analysis method was used, and factor analysis method was used to extract the main factors associated with service capabilities as well as to calculate a composite score. The t-test of two independent-samples methods was used to comparison analyze. Results: The differences of common factor scores (hospital scale and service capacity, treatment quality, service quality, and services efficiency) between pilot and non-pilot hospitals were not statistically significant (P>0.05). The service capability score in 2012 was better than that in 2009 either in pilot or non-pilot group (P<0.05). The pilot hospitals' service capability score was better than that in non-pilot groups either in 2010 or 2012 (P<0.05). However, the differences from 2009 to 2012 of service capability score between pilot and non-pilot hospitals were not statistically significant. Conclusion: The comprehensive service capability of both pilot and non-pilot group all got improvement. However, county public hospital reform did not significantly play a due good role in improving the service capability in pilot group. The reform was helpful to improve the hospital current situation, but it has not completely achieved policy objectives in the sample hospitals of this study.

14.
Forensic Sci Int ; 291: 109-114, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30195151

RESUMO

China is a multi-ethnic country. Due to its diverse terrain, many ethnic groups are geographically isolated within China. This phenomenon is especially prevalent in southern China. As Y-STR loci are paternally inherited, they can be used to effectively understand the genetic relationship between different populations and thus aid in forensic science. In this study, forty Y-STR loci were analysed in 2018 unrelated male individuals from the following seven ethnic populations in South China: Yao (n=147), Zhuang (n=225), Gelao (n=156), Miao (n=186), Maonan (n=133), Gin (n=160) and Guangxi Han (n=1011). Using both AGCU Y24 STR and GFS Y24 STR genotyping kits, a total of 335 alleles and 141 haplotypes of three multi-copy loci were observed in these seven populations. The highest GD value of the 40 Y-STR loci in the overall population was 0.9643 for DYS385a/b, while the lowest was 0.4101 for DYS438. Out of the 2018 samples analysed, 1935 distinct haplotypes and 1858 unique haplotypes were observed. The HD value of the total samples was up to 0.9994 and ranged from a low of 0.9908 in the Yao to a high of 0.9999 in the Han population. We found using population structure analysis that the genetic distance is smaller among the seven southern populations (Guangxi Han, Gelao, Yao, Miao, Zhuang, Jing and Maonan) than the northern populations (Tibetan, Korean, Mongolian, Uygur and Hui). We show that the 40 Y-STRs have a high level of polymorphism in the South China ethnic groups and there is a high degree of differentiation among ethnic groups located in geographically distributed and densely populated areas. These data may provide additional resources for forensic applications and population genetic studies.


Assuntos
Cromossomos Humanos Y , Grupos Étnicos/genética , Genética Populacional , Repetições de Microssatélites , Polimorfismo Genético , China , Impressões Digitais de DNA , Haplótipos , Humanos , Masculino , Reação em Cadeia da Polimerase
15.
Leg Med (Tokyo) ; 27: 38-42, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28697408

RESUMO

Trisomy X (47, XXX) is a sex chromosome aneuploidy condition in which females have an extra X chromosome, compared to the 46, XX karyotype in typical females. There is considerable variation in the phenotype, with some individuals very mildly affected and others with more significant physical and psychological features. However, the trisomy X in this case, without any of these phenotype, is rarely reported. Here, we report a case found during DNA sample collection in a study of genetic polymorphism analysis of loci in Chinese ethnic group, of a female with neither laboratory or clinical signs of Triple X syndrome. She was born at her mother's 60years old and her father's 62years old. Advanced maternal age was found acting as a significant risk factor of Triplo-X. Moreover, her child are also born without manifestations of 47, XXX syndrome. Pedigree study demonstrated the normal karyotype of the children. A diagnosis of 47XXX was made on the basis of a chromosomal study. Therefore, laboratory investigations (including PCR amplification, more than two kinds of X-STR genotyping, G-banding karyotyping analysis and Pedigree study) are applied to rule out the possibility of Mosaicism (45, X0/47, XXX) and ascertain her 47XXX karyotype without mosaic. The objective of this study was to report a case of trisomy X, diagnostic investigation and management of the case, and to analysis the genetically possible reasons behind the case. To our knowledge, this case is a rare one, found in DNA sample collection for the estimation of gene frequency in the process of genetic polymorphism study, of non-mosaic 47, XXX without signs of physical syndrome and born healthy children. In this study, it revealed that the proportion of trisomy X would be more than official statistics and risk of systemic disabilities is lower than estimated. Moreover, we found out that sample mixture and mosaicism act as the interference factors in forensic test. Therefore, we draw the conclusion that attentions and certain improved methods should be applied to the diagnosis of non-mosaic triple X, which is of great significance in decreasing the interruptions in the whole process of forensic and paternity identification.


Assuntos
Polimorfismo Genético , Transtornos do Cromossomo Sexual no Desenvolvimento Sexual/diagnóstico , Transtornos do Cromossomo Sexual no Desenvolvimento Sexual/genética , Trissomia/diagnóstico , Trissomia/genética , Cromossomos Humanos X/genética , Genética Forense , Genótipo , Humanos , Mosaicismo , Polimorfismo Genético/genética , Aberrações dos Cromossomos Sexuais , Transtornos do Cromossomo Sexual no Desenvolvimento Sexual/fisiopatologia , Trissomia/fisiopatologia
16.
Sci Rep ; 7: 42782, 2017 02 17.
Artigo em Inglês | MEDLINE | ID: mdl-28211539

RESUMO

A new 19 X- short tandem repeat (STR) multiplex PCR system has recently been developed, though its applicability in forensic studies has not been thoroughly assessed. In this study, 932 unrelated individuals from four Chinese ethnic groups (Han, Tibet, Uighur and Hui) were successfully genotyped using this new multiplex PCR system. Our results showed significant linkage disequilibrium between markers DXS10103 and DXS10101 in all four ethnic groups; markers DXS10159 and DXS10162, DXS6809 and DXS6789, and HPRTB and DXS10101 in Tibetan populations; and markers DXS10074 and DXS10075 in Uighur populations. The combined powers of discrimination in males and females were calculated according to haplotype frequencies from allele distributions rather than haplotype counts in the relevant population and were high in four ethnic groups. The cumulative powers of discrimination of the tested X-STR loci were 1.000000000000000 and 0.999999999997940 in females and males, respectively. All 19 X-STR loci are highly polymorphic. The highest Reynolds genetic distances were observed for the Tibet-Uighur pairwise comparisons. This study represents an extensive report on X-STR marker variation in minor Chinese populations and a comprehensive analysis of the diversity of these 19 X STR markers in four Chinese ethnic groups.


Assuntos
Grupo com Ancestrais do Continente Asiático/genética , Cromossomos Humanos X/genética , Genética Forense/métodos , Repetições de Microssatélites , Grupo com Ancestrais do Continente Asiático/etnologia , Feminino , Técnicas de Genotipagem/métodos , Humanos , Masculino , Reação em Cadeia da Polimerase Multiplex/métodos , Reação em Cadeia da Polimerase Multiplex/normas
17.
Springerplus ; 5(1): 1877, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27833836

RESUMO

BACKGROUND: As healthcare spending continues to increase, medical insurance is now under great pressure of growing economic burden. To control the excessive growth of medical expenditure, change of medical payment system was clearly put forward in China's new healthcare reform. With this end, Tianjin, a large city in North China, is now exploring to replace traditional fee-for-service (FFS) with global budget payment system (GBPS), and actual effects of GBPS needs to be assessed. METHODS: Data of this study is from the 2013 National Health Services Utilization Survey among patients of Urban Basic Medical Insurance in China, containing 102,492 outpatient visits of 21,925 hypertensive patients to Tianjin's primary hospitals in 2013. t test was used to compare the difference between continuous variables. A linear regression analysis was also done to identify possible risk factors of medical expenditure. RESULTS: On the basis of expenditure per capital, GBPS, compared with FFS, has significantly reduced total medical expense (CNY 640.28 vs. CNY 700.64, p < 0.001), medical insurance (MI) fund expense (CNY 491.87 vs. CNY 532.37, p < 0.001) and out-of-pocket (OOP) expense (CNY 148.42 vs. CNY 168.27, p < 0.001). Results of generalized linear regression also show that younger people, female and GBPS independently predict less total medical expense, MI fund expense and OOP expense. CONCLUSIONS: Compared with FFS, GBPS can help reduce total medical expense, MI fund expense and OOP expense significantly. This study offers evidence for wider implementation of GBPS in China.

18.
Electrophoresis ; 37(15-16): 2260-72, 2016 08.
Artigo em Inglês | MEDLINE | ID: mdl-27184937

RESUMO

The 19 X-STRs multiplex system is a PCR-based amplification kit that facilitates simultaneous amplification of 19 X-chromosomal STR loci (i.e. DXS7423, DXS10148, DXS10159, DXS6809, DXS7424, DXS8378, DXS10164, DXS10162, DXS7132, DXS10079, DXS6789, DXS101, DXS10103,DXS10101, HPTRB, DXS10075, DXS10074, DXS10135, and DXS10134). Eleven loci were extensively used in an Investigator Qiagen Argus X-12 (DXS7423, DXS10148, DXS8378, DXS10162, DXS7132, DXS10079, DXS10103, DXS10101, HPTRB, DXS10074, and DXS10135). In this research, the multiplex system was tested for detection sensitivity, DNA mixtures, inhibitor tolerance and species specificity; SWGDAM Validation Guidelines - Approved December 2012 were followed for the human fluorescent STR multiplex PCR reagent. Samples from 181 unrelated Zhejiang Han individuals (121 males and 60 females) were typed using this multiplex system. The results show that this 19X-STRs multiplex system is a robust and reliable amplification means to facilitate forensic and human identification testing.


Assuntos
Cromossomos Humanos X , Impressões Digitais de DNA/métodos , Genética Populacional/métodos , Reação em Cadeia da Polimerase Multiplex/métodos , Animais , China , Feminino , Testes Genéticos , Humanos , Masculino , Sensibilidade e Especificidade , Especificidade da Espécie
19.
Leg Med (Tokyo) ; 17(6): 489-92, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26593995

RESUMO

AmpFℓSTR Sinofiler PCR Amplification Kit is specially developed for Chinese forensic laboratories, but there are little population-genetic data about this kit for Southern China. This kit contains 15 STR loci: D8S1179, D21S11, D7S820, CSF1PO, D3S1358, D13S317, D16S539, D2S1338, D19S433, vWA, D18S51, D6S1043, D12S391, D5S818 and FGA. We have conducted genotyping experiments on the 15 STR loci in 5234 unrelated individuals from Guangdong (Southern China). We observed a total of 243 alleles in the group with the allelic frequency values ranging from less than 0.0001 to 0.3686. Our statistic analysis indicates that the 15 STR loci conform to the Hardy-Weinberg's equilibrium (p>0.05). The highest polymorphism was found at D6S1043 locus and the lowest was found at D3S1358. The combined power of discrimination reached 0.99999999999999999977431 and the combined probability of paternity exclusion reached 0.999999721 for 15 STR loci. Guangdong Han population had significant differences compared with Shaanxi, Shandong and Henan province of Northern China. A Neighbor-joining tree indicates that the Guangdong Han has a close genetic relationship with the Yunnan population. Significant differences were found between Guangdong Han population and other reported populations (Japanese, Philippine, African American, Caucasian, Hispanic and Western Romanian) at 2-11 STR loci. The results may provide useful information for forensic sciences and population genetics studies. The present findings indicate that all the 15 STR loci are highly genetically polymorphic in the Han population of Guangdong.


Assuntos
Grupo com Ancestrais do Continente Asiático/genética , Repetições de Microssatélites , Polimorfismo Genético , China , Frequência do Gene , Técnicas de Genotipagem , Humanos
20.
Inorg Chem ; 54(21): 10214-23, 2015 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-26468668

RESUMO

This report describes the design, synthesis, solubility, and electrochemistry of a series of tris-bipyridine chromium complexes that exhibit up to six reversible redox couples as well as solubilities approaching 1 M in acetonitrile. We have systematically modified both the ligand structure and the oxidation state of these complexes to gain insights into the factors that impact solubility and electrochemistry. The results provide a set of structure-solubility-electrochemistry relationships to guide the future development of electrolytes for nonaqueous flow batteries. In addition, we have identified a promising candidate from the series of chromium complexes for further electrochemical and battery assessment.

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