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1.
Chem Commun (Camb) ; 2021 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-34636370

RESUMO

MAO-A promotes the proliferation of human glioma cells. Herein, we report a series of MAO-A specific two-photon small molecular fluorescent probes (A1-5) based on an intramolecular charge transfer enhancing strategy. The activity of endogenous MAO-A can be selectively imaged using A3 as a representative probe in different biological samples including human glioma cells/tissues via two-photon fluorescence microscopy. The study provides new tools for the visual detection of glioma.

2.
Talanta ; 235: 122719, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34517587

RESUMO

Here, a dual lock-and-key fluorescence probe was developed for visualizing the inflammatory process in myocardial H9C2 cells. The probe possessed two-photon properties, viscosity sensitivity, and hydrogen peroxide (H2O2) responsiveness. A thiocarbamate spacer between fluorophore and H2O2 responsive unit enabled the release of carbonyl sulfide (COS). This rapidly converts to the anti-inflammatory hydrogen sulfide (H2S) by the ubiquitous enzyme carbon anhydrase. The probe displayed a dual response towards hydrogen peroxide and viscosity in vitro. No obvious fluorescence changes were observed towards either hydrogen peroxide or viscosity alone. In cellular experiments, the probe demonstrated good biocompatibility, low toxicity, and was shown responses towards exogenous and endogenous hydrogen peroxide under viscosity conditions. LPS induced cell inflammation showed it was able to effectively alleviate the inflammation-caused damage by releasing H2S and eliminating H2O2. The new protocol demonstrates its promising to achieve diagnosis and treatment of cellular inflammatory process.


Assuntos
Corantes Fluorescentes , Sulfeto de Hidrogênio , Fluorescência , Células HeLa , Humanos , Peróxido de Hidrogênio , Inflamação/tratamento farmacológico , Viscosidade
3.
Nano Lett ; 2021 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-33913710

RESUMO

Colloidal CdSe nanoplatelets (NPLs) have substantial potential in light-emitting applications because of their quantum-well-like characteristics. The self-trapped state (STS), originating from strong electron-phonon coupling (EPC), is promising in white light luminance because of its broadband emission. However, achieving STS in CdSe NPLs is extremely challenging because of their intrinsic weak EPC nature. Herein, we developed a strong STS emission in the spectral range of 450-600 nm by building superlattice (SL) structures with colloidal CdSe NPLs. We demonstrated that STS is generated via strong coupling of excitons and zone-folded longitudinal acoustic phonons with formation time of ∼450 fs and localization length of ∼0.56 nm. The Huang-Rhys factor, describing the EPC strength in SL structure, is estimated to be ∼19.9, which is much larger than that (∼0.1) of monodispersed CdSe NPLs. Our results provide an in-depth understanding of STS and a platform for generating and manipulating STS by designing SL structures.

4.
Lasers Med Sci ; 36(8): 1671-1679, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33486651

RESUMO

For over several decades, 595-nm pulsed dye laser (PDL) has been used effectively, reducing erythema and improving the pliability and texture of burn scars. Children usually tolerate PDL treatment as it is non-invasive and causes only mild pain compared to other laser treatments. However, currently, there are limited data on scar management in children who underwent PDL treatment, especially for Fitzpatrick skin types III and IV. The objective of the study was to identify the optimal parameters for the PDL treatment that induce inhibitory effects on scar tissue in children with Fitzpatrick skin types III and IV. Besides, the study assessed the usefulness of high-frequency ultrasound (20 MHz) and laser Doppler flowmetry in assessing these lesions. A total of 165 (79 males and 86 females) children with hypertrophic scars treated by PDL were assessed by the Vancouver scar scale (VSS), high-frequency ultrasound (20 MHz), and laser Doppler flowmetry. The parameters used for the 595-nm PDL treatment were pulse duration of 0.45 ms, fluence between 5 and 9 J/cm2, a spot size of 7 mm, and treatment intervals from 3 to 8 weeks. There were no significant differences between pretreatment and post-treatment in terms of the distribution of sex, type of skin color, and low and high fluences. While the mean scores of all scar parameters based on VSS, except thickness and pliability between pre and post-treatment, showed significant differences in ≤3-year-old children vs. to >3-year-old children, except for the subscore, a significant improvement was observed when PDL was initiated within 4 to 6 months of the scar age. In Chinese children with Fitzpatrick skin types III and IV, early intervention, appropriate treatment intervals, and low fluence of PDL were optimal parameters in treating hypertrophic burn scars. The combined high-frequency ultrasound and laser Doppler flowmetry assessment of scars helped assess these lesions and compare the efficacy of different treatment modalities.

5.
J Extracell Vesicles ; 9(1): 1816710, 2020 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-33133429

RESUMO

Therapeutically intervening the function of RNA in vivo remains a big challenge. We here developed an exosome-based strategy to deliver engineered RNA-binding protein for the purpose of recruiting specific RNA to the lysosomes for degradation. As a proof-of-principle study, RNA-binding protein HuR was fused to the C-terminus of Lamp2b, a membrane protein localized in both exosome and lysosome. The fusion protein was able to be incorporated into the exosomes. Moreover, exosomes engineered with Lamp2b-HuR successfully decreased the abundance of RNA targets possibly via lysosome-mediated degradation, especially when the exosomes were acidified. The system was specifically effective in macrophages, which are lysosome enriched and resistant to routine transfection mediated RNAi strategy. In the CCl4-induced liver injury mouse model, we found that delivery of acidified exosomes engineered with Lamp2b-HuR significantly reduced liver fibrosis, together with decreased miR-155 and other inflammatory genes. In summary, the established exosome-based RNA-binding protein delivery strategy, namely "exosome-mediated lysosomal clearance", takes the advantage of exosome in targeted delivery and holds great promise in regulating a set of genes in vivo.

6.
J Mol Histol ; 51(4): 467, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32617897

RESUMO

In the original publication of the article, the name of one of the corresponding authors was published incorrectly. The name should be 'Ke Tao' instead of 'Kao Tao'. The corrected author group is given in this Correction.

7.
J Mol Histol ; 51(4): 375-383, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32556903

RESUMO

Accelerating wound healing is a key consideration for surgeons. The three stages of wound healing include the inflammatory response, cell proliferation and tissue repair, and much research has focused on the migration and proliferation of epidermal cells, since this is one of the most important steps in wound healing. Studies have shown that adipose mesenchymal stem cells (ADSCs) can promote wound healing by releasing exosomes, although the specific mechanism remains unclear. To clarify the role of adipose mesenchymal stem cell exosomes (ADSCs-exo), we constructed a HaCaT cells model and a mouse wound healing model to examine the effects of ADSCs-exo on wound healing. CCK8 assays and the scratch test showed that ADSCs-exo could promote the proliferation and migration of HaCaT cells. Western blotting and real-time PCR showed that ADSCs-exo upregulated the phosphorylation of AKT and the expression of HIF-1α in HaCaT cells. HIF-1α expression was reduced by inhibiting AKT phosphorylation,and the migration of HaCaT cells simultaneously slowed. These results were also confirmed in vivo. In conclusion, we confirmed that ADSCs-exo promote the proliferation and migration of HaCaT cells by regulating the activation of the AKT/HIF-1α signaling pathway, thus promoting wound healing.


Assuntos
Tecido Adiposo/fisiologia , Movimento Celular/fisiologia , Proliferação de Células/fisiologia , Exossomos/fisiologia , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Células-Tronco Mesenquimais/fisiologia , Proteínas Proto-Oncogênicas c-akt/metabolismo , Cicatrização/fisiologia , Tecido Adiposo/metabolismo , Adulto , Animais , Linhagem Celular , Exossomos/metabolismo , Feminino , Fibroblastos/metabolismo , Fibroblastos/fisiologia , Humanos , Queratinócitos/metabolismo , Queratinócitos/fisiologia , Células-Tronco Mesenquimais/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Modelos Animais , Transdução de Sinais/fisiologia , Pele/metabolismo , Pele/fisiopatologia , Regulação para Cima/fisiologia , Adulto Jovem
8.
Shock ; 54(6): 819-827, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-32496418

RESUMO

Na/H exchanger 1 (NHE1) is a ubiquitously expressed protein on mammalian plasma membranes and involved in cell apoptosis and tissue injury. Our previous study found that NHE1 inhibition prevents burn-induced acute lung injury (ALI). However, the potential mechanism of NHE1 in burn-induced ALI is still unclear. This study investigated the role of NHE1 in burn-induced apoptosis of human pulmonary microvascular endothelial cells. Based on the western blot analyses, real-time PCR, fluorescence spectroscopy, and apoptosis analysis, we found that burn serum significantly induced NHE1 activation, promoted intracellular Na accumulation, and elevated apoptosis ratio. Inhibition of NHE1 with cariporide reversed burn-induced intracellular Na accumulation and cell apoptosis. Different doses of cariporide also significantly decreased Cai concentrations and calpain activity induced by burn serum. Furthermore, inhibition of PI3K contributed to the increase of NHE1 activation and cell apoptosis, whereas the inhibition of p38 MAPK led to inhibition of NHE1 activation and significant decreases of cell apoptosis. The data demonstrate that NHE1 activation facilitates burn-induced endothelial cell apoptosis, mediated by Ca-dependent pathway. PI3K-Akt and p38 MAPK were found to be upstream regulators of NHE1. This study provides new mechanisms underlying burn-induced ALI.

9.
Burns ; 46(6): 1373-1380, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-32014349

RESUMO

BACKGROUND: The dysbiosis of gastrointestinal microbiome is an important reason for burn-induced intestinal injury. Clostridium butyricum (C.butyricum) and its production butyrate are beneficial for the homeostasis of intestinal microflora and suppression of inflammatory response. PURPOSE: The roles of C.butyricum and butyrate in burn-induced intestinal injury were explored. The effects of oral administration of C.butyricum on intestinal injury were observed in burned mice. MATERIALS AND METHODS: The skin surface of mice was exposed to 95 °C water to induce a burn injury. Then the intestinal microbiome structure, abundance of C.butyricum and level of butyrate were respectively observed. The correction between intestinal permeability indicated by FITC dextran level and abundance of C.butyricum or level of butyrate was analyzed. C.butyricum was cultured and orally administrated to burned mice. The levels of butyrate, FITC dextran and pro-inflammatory cytokines, including interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α) were respectively measured. RESULTS: Burn injury altered the intestinal microbiome structure of mice, and especially decreased the abundance of C.butyricum and level of butyrate. Both the abundance of C.butyricum and the level of butyrate were negatively correlated with the intestinal permeability. Oral administration of C.butyricum increased the level of butyrate, decreased levels of TNF-α and IL-6, and suppressed intestinal damage in burn-injured mice. CONCLUSION: Oral administration of C.butyricum significantly alleviated the intestinal damage induced by burn injury. The therapeutic effects of C.butyricum and butyrate on burn injury should be further explored, which deserves further investigation.

10.
Mol Cell Probes ; 51: 101543, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32105703

RESUMO

Deformities in human soft tissue caused by trauma or burn present a difficult problem in plastic surgery. In this study, we encapsulated troglitazone and angiotensin 1-7 mimetic AVE0991 in gelation microspheres with the goal of inducing epithelial transformation for potential applications in tissue reconstruction. After troglitazone or AVE0991 were encapsulated to gelation microspheres, their release kinetics and bioactivity were examined. Surface morphology and diameter of the gelation microspheres were evaluated using light microscopy. The release of the drugs was assessed in the presence of human adipose-derived stem cells (ADSCs). Treatment with troglitazone microspheres increased cell viability and activated the ß-catenin in ADSCs. Moreover, the AVE0991 microspheres also increased cell viability and C-myc expression of ADSCs. These results showed that troglitazone and AVE0991 microspheres promoted the activity of ADSCs. Furthermore, ADSCs were co-treated with troglitazone and AVE0991 microspheres. Western blot and immunofluorescent staining showed that co-treatment with troglitazone and AVE0991 microspheres elevated the expression of epithelialization associated protein CK14 in ADSCs. In conclusion, our findings indicate that microspheres with troglitazone and AVE0991 can significantly improve the viability and epithelialization of ADSCs, which provides a new approach for the construction of tissue-engineered skin.


Assuntos
Gelatina/química , Imidazóis/farmacocinética , Células-Tronco Mesenquimais/efeitos dos fármacos , Engenharia Tecidual/métodos , Troglitazona/farmacocinética , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Liberação Controlada de Fármacos , Humanos , Hipoglicemiantes/farmacologia , Imidazóis/farmacologia , Células-Tronco Mesenquimais/metabolismo , Microesferas , Tamanho da Partícula , Proteínas Proto-Oncogênicas c-myc/genética , Proteínas Proto-Oncogênicas c-myc/metabolismo , Reepitelização , Reação em Cadeia da Polimerase em Tempo Real , Troglitazona/farmacologia , beta Catenina/metabolismo
11.
Angew Chem Int Ed Engl ; 59(19): 7536-7541, 2020 05 04.
Artigo em Inglês | MEDLINE | ID: mdl-32077158

RESUMO

Monoamine oxidases have two functionally distinct but structurally similar isoforms (MAO-A and MAO-B). The ability to differentiate them by using fluorescence detection/imaging technology is of significant biological relevance, but highly challenging with available chemical tools. Herein, we report the first MAO-A-specific two-photon fluorogenic probe (F1), capable of selective imaging of endogenous MAO-A enzymatic activities from a variety of biological samples, including MAO-A-expressing neuronal SY-SY5Y cells, the brain of tumor-bearing mice and human Glioma tissues by using two-photon fluorescence microscopy (TPFM) with minimal cytotoxicity.


Assuntos
Neoplasias Encefálicas/enzimologia , Corantes Fluorescentes/síntese química , Glioma/enzimologia , Monoaminoxidase/química , Animais , Linhagem Celular , Desenho de Fármacos , Humanos , Camundongos , Microscopia de Fluorescência por Excitação Multifotônica , Neurônios/enzimologia
12.
Neurochem Int ; 134: 104671, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-31926197

RESUMO

Parkinson's disease (PD), the second most common chronic neurodegenerative disorder, broadly remains incurable. Both genetic susceptibility and exposure to deleterious environmental stimuli contribute to dopaminergic neuron degeneration in the substantia nigra. Hence, reagents that can ameliorate the phenotypes rendered by genetic or environmental factors should be considered in PD therapy. In this study, we found that polydatin (Pol), a natural compound extracted from grapes and red wines, significantly attenuated rotenone- (Rot) or Parkin deficiency-induced mitochondrial dysfunction and cell death in SH-SY5Y, a human dopaminergic neuronal cell line. We showed that Pol significantly attenuated the Rot-induced decrease in cell viability, mitochondrial membrane potential (MMP), and Sirt 1 expression and increase in cell death, reactive oxygen species (ROS) and DJ1 expression. Rot resulted in a decrease in mTOR/Ulk-involved autophagy and an increase in PGC1ß/mfn2-involved mitochondrial fusion, which was inhibited by Pol. We further demonstrated that the protective effects of Pol are partially blocked when autophagy-related gene 5 (Atg5) is genetically inactivated, suggesting that Pol-mediated neuroprotection requires Atg5. Moreover, Pol rescued Parkin knockdown-induced oxidative stress, mitochondrial dysfunction, autophagy impairment, and mitochondrial fusion enhancement. Interestingly, Pol treatment could also rescue the mitochondrial morphological abnormality and motorial dysfunction of a Drosophila PD model induced by Parkin deficiency. Thus, Pol could represent a useful therapeutic strategy as a disease-modifier in PD by decreasing oxidative stress and regulating autophagic processes and mitochondrial fusion.


Assuntos
Proteína 5 Relacionada à Autofagia/metabolismo , Autofagia/efeitos dos fármacos , Doença de Parkinson/tratamento farmacológico , Rotenona/farmacologia , Ubiquitina-Proteína Ligases/metabolismo , Autofagia/fisiologia , Morte Celular/efeitos dos fármacos , Neurônios Dopaminérgicos/metabolismo , Humanos , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Potencial da Membrana Mitocondrial/fisiologia , Fármacos Neuroprotetores/farmacologia , Doença de Parkinson/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Ubiquitina-Proteína Ligases/efeitos dos fármacos
13.
Nat Nanotechnol ; 15(3): 192-197, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31959929

RESUMO

Chirality-the property of an object wherein it is distinguishable from its mirror image-is of widespread interest in chemistry and biology1-6. Regioselective magnetization of one-dimensional semiconductors enables anisotropic magnetism at room temperature, as well as the manipulation of spin polarization-the properties essential for spintronics and quantum computing technology7. To enable oriented magneto-optical functionalities, the growth of magnetic units has to be achieved at targeted locations on a parent nanorod. However, this challenge is yet to be addressed in the case of materials with a large lattice mismatch. Here, we report the regioselective magnetization of nanorods independent of lattice mismatch via buffer intermediate catalytic layers that modify interfacial energetics and promote regioselective growth of otherwise incompatible materials. Using this strategy, we combine materials with distinct lattices, chemical compositions and magnetic properties, that is, a magnetic component (Fe3O4) and a series of semiconducting nanorods absorbing across the ultraviolet and visible spectrum at specific locations. The resulting heteronanorods exhibit optical activity as induced by the location-specific magnetic field. The regioselective magnetization strategy presented here enables a path to designing optically active nanomaterials for chirality and spintronics.

14.
Shock ; 54(3): 337-346, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-31626039

RESUMO

Genistein (Gen) exhibits strong anti-oxidative/antinitrative activity and cardioprotective effects in several models; however, its role in burn-induced myocardial injury is unknown. This study investigated the protective effect of Gen on burn-induced myocardial injury and aimed to elucidate the mechanism of protection. Mice were injected with Gen, intraperitoneally, at different dose immediately after burn injury. The expression levels of Notch-1 intracellular domain (NICD1) and hairy and enhancer of split (Hes-1) were determined by immunoblotting. Conditional Notch-RBP-J knockout mice were used to investigate the mechanisms of Gen-induced cardioprotection. Gen alleviated burn-induced myocardial injury, as shown by improved left ventricle ejection fraction, decreased serum lactate dehydrogenase and creatine kinase levels, and apoptosis. Moreover, Gen decreased expressions of inducible nitric oxide (NO) synthase and gp, reduced NO and superoxide anions production, and ameliorated their cytotoxic reaction product, peroxynitrite. More importantly, Gen significantly up-regulated the expression of NICD1 and Hes1 after burn injury. In addition, genetic knockout of Notch1 not only blocked the cardioprotection of Gen but also markedly attenuated Gen-induced anti-oxidative/antinitrative effect. These results demonstrate, for the first time, that Gen treatment attenuates burn-induced myocardial injury via the Notch1 mediated suppression of oxidative/nitrative stress.

15.
Ann Plast Surg ; 84(5): 525-528, 2020 05.
Artigo em Inglês | MEDLINE | ID: mdl-31609252

RESUMO

BACKGROUND: Reconstruction of distal foot defect remains a challenge in plastic surgery. The purpose of this report is to present a new procedure that repairs these defects in severe burn patients. Results of application and follow-up in 7 patients were presented. METHODS: From January 2016 to March 2018, a total of 7 patients (age ranging from 21 to 57 years) with distal foot defects were treated in our department. All the wounds were caused by severe burns and repaired by the free vascularized fascia lata combined with thin split-skin graft. After the operation, the status of the fascia flaps and grafted skin was observed, and follow-up information and complications were documented. RESULTS: Among the 7 patients, the flaps and grafted skins completely survived in 5 patients. One patient was found to have grafted skin necrosis in the perioperative period, and 1 patient was found to have partial flap necrosis in the follow-up period. After conventional dressing treatment and skin grafting, the wounds healed in both patients. The mean follow-up was 6 months. CONCLUSIONS: The method of combining the free vascularized fascia lata with thin split-skin graft represents a satisfactory approach for the repairing of distal foot defects.


Assuntos
Procedimentos Cirúrgicos Reconstrutivos , Lesões dos Tecidos Moles , Adulto , Fascia Lata , Humanos , Pessoa de Meia-Idade , Transplante de Pele , Lesões dos Tecidos Moles/cirurgia , Retalhos Cirúrgicos , Adulto Jovem
16.
Immunol Cell Biol ; 98(2): 127-137, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31811786

RESUMO

Sepsis is a complex inflammatory disorder in which high mortality is associated with an excessive inflammatory response. Inhibitor of growth 4 (ING4), which is a cofactor of histone acetyltransferase and histone deacetylase complexes, could negatively regulate this inflammation. However, the exact molecular signaling pathway regulated by ING4 remains uncertain. As a pivotal histone deacetylase, Sirtuin1 (SIRT1), which is widely accepted to be an anti-inflammatory molecule, has not been found to be linked to ING4. This study investigated how ING4 is involved in the regulation of inflammation by constructing lipopolysaccharide (LPS)-induced macrophage and mouse sepsis models. Our results revealed that ING4 expression decreased, whereas the levels of proinflammatory cytokines increased in LPS-stimulated cultured primary macrophages and RAW 264.7 cells. ING4 transfection was confirmed to alleviate the LPS-induced upregulation of proinflammatory cytokine expression both in vitro and in vivo. In addition, ING4-overexpressing mice were hyposensitive to an LPS challenge and displayed reduced organ injury. Furthermore, immunoprecipitation indicated a direct interaction between ING4 and the SIRT1 protein. Moreover, ING4 could block nuclear factor-kappa B (NF-κB) P65 nuclear translocation and restrict P65 acetylation at lysine 310 induced by LPS treatment. These results are the first to clarify that the anti-inflammatory role of ING4 is associated with SIRT1, through which ING4 inhibits NF-κB signaling activation. Our studies provide a novel signaling axis involving ING4/SIRT1/NF-κB in LPS-induced sepsis.


Assuntos
Proteínas de Transporte/metabolismo , Inflamação/metabolismo , Macrófagos/metabolismo , NF-kappa B/metabolismo , Sepse/metabolismo , Sirtuína 1/metabolismo , Proteínas Supressoras de Tumor/metabolismo , Acetilação , Animais , Proteínas de Transporte/genética , Inflamação/induzido quimicamente , Inflamação/genética , Lipopolissacarídeos , Macrófagos/efeitos dos fármacos , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Proteínas dos Microfilamentos/metabolismo , Ligação Proteica , Células RAW 264.7 , Sepse/genética , Sepse/patologia , Transdução de Sinais/genética , Sirtuína 1/genética , Proteínas Supressoras de Tumor/genética , Regulação para Cima
17.
ACS Appl Mater Interfaces ; 11(34): 31421-31426, 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31389682

RESUMO

Development of chiral metal-organic frameworks (MOFs) for circularly polarized luminescence (CPL) is a challenging but important task. In this work, we report a first example of azapyrene-based chiral MOF thin films [Zn2Cam2DAP]n grown on functionalized substrates (named SURchirMOF-4) for CPL property. By using a liquid-phase epitaxial layer-by-layer method, the resulted SURchirMOF-4 was constructed from chiral camphoric acid and 2,7-diazapyrene ligand, which has high orientation and homogeneity. The circular dichroism, CPL, and enantioselective adsorption results show that SURchirMOF-4 has strong chirality and CPL property as well as good enantioselective adsorption toward enantiomers of methyl-lactate. The synthesis of azapyrene-based chiral MOF thin films not only represents an ideal model for studying the enantioselective adsorption, but also will be a valuable approach for development of the chiral thin film exhibiting CPL property.

18.
J Am Chem Soc ; 141(35): 13700-13707, 2019 09 04.
Artigo em Inglês | MEDLINE | ID: mdl-31408600

RESUMO

Chiral colloidal semiconductor nanocrystals (NCs) are an emerging type of chiral materials. These chiral NCs exhibit unique quantum confinement-determined optical activity and have aroused much interest in the multidisciplinary fields of chemistry, physics and biology. Herein, the state-of-the-art progresses of their rational synthesis, fundamental understanding and potential application are summarized. In addition, a personal view about the future development of chiral semiconductor NCs is offered.

19.
J Plast Surg Hand Surg ; 53(6): 356-360, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31268389

RESUMO

The treatment of donor sites after split-thickness skin grafting (STSG) is a routine operation step, and complications at the donor site due to improper operation and care are unwelcome. This study evaluates whether the use of platelet-rich plasma (PRP) applied at the STSG area promotes wound healing and improves scar development. Clinical data of 30 patients who underwent STSG operations between January 2016 and January 2017 for various reasons were retrospectively analyzed. These 30 patients received two treatments and the data were summed up in two groups: the PRP group, which was the study group, included patients who received traditional petrolatum gauze dressing with PRP gel at the donor sites. The petrolatum gauze group, which was the control group, received only petrolatum gauze care without PRP gel. The time and frequency of dressing change were comparable between the two groups, and the mean wound healing times in the PRP group and petrolatum gauze group were 13.89 ± 4.65 and 17.73 ± 5.06 days, respectively, and the difference was statistically significant (p < 0.05). In addition, the total Vancouver scar scale (VSS) scores of the PRP group at 4, 12 and 52 weeks were 6.41 ± 0.77, 4.42 ± 0.43 and 2.41 ± 0.39, respectively, which were statistically significantly lower (p < 0.05) than those of the control group at 7.67 ± 0.64, 6.28 ± 0.62 and 4.29 ± 0.64, respectively. The use of PRP gel can promote wound healing, relieve scar development and alleviate pain at the donor site after STSG.


Assuntos
Cicatriz/prevenção & controle , Plasma Rico em Plaquetas , Sítio Doador de Transplante , Cicatrização , Adulto , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Estudos Retrospectivos , Transplante de Pele , Transplante Autólogo
20.
Nanoscale ; 11(41): 19380-19386, 2019 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-31204749

RESUMO

The evolution of electronic states of nanocrystals under shape variation is hardly detected by conventional optical and electronic instruments due to the condensed electronic levels of nanocrystals. Herein, we demonstrate that magnetic circular dichroism (MCD) spectroscopy is a high-resolution method to monitor this delicate progress on account of the sensitive Zeeman response to electronic states. In particular, the MCD intensity of the first excitonic transition exponentially decreases with the shape changing from quantum dots to quantum rods owing to the increased density of valence pz state with elongation in the z direction, which contributes much less to MCD intensity compared with p±. This work provides a simple but effective strategy for understanding the electronic state evolution in various semiconductor nanomaterials.

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