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1.
Artigo em Inglês | MEDLINE | ID: mdl-34636536

RESUMO

Although organic artificial enzymes have been reported as biomimetic oxidation catalysts and are widely used for colorimetric biosensors, developing organic artificial enzymes with high enzymatic activity is still a challenge. Two-dimensional (2D) covalent organic frameworks (COFs) have shown superior potential in biocatalysts because of their periodic π-π arrays, tunable pore size and structure, large surface area, and thermal stability. The interconnection of electron acceptor and donor building blocks in the 2D conjugated COF skeleton can lead to narrower band gaps and efficient charge separation and transportation and thus is helpful to improve catalytic activity. Herein, a donor-acceptor 2D COF was synthesized using tetrakis(4-aminophenyl)pyrene (Py) as an electron donor and thieno[3,2-b]thiophene-2,5-dicarbaldehyde (TT) as an electron acceptor. Under visible light irradiation, the donor-acceptor 2D COF exhibited superior enzymatic catalytic activity, which could catalyze the oxidation of chromogenic substrates such as 3,3',5,5'-tetramethylbenzidine (TMB) by the formation of superoxide radicals and holes. Based on the above property, the photoactivated donor-acceptor 2D COF with enzyme-like catalytic properties was designed as a robust colorimetric probe for cheap, highly sensitive, and rapid colorimetric detection of glutathione (GSH); the corresponding linear range of GSH was 0.4-60 µM, and the limit of detection was 0.225 µM. This study not only presents the construction of COF-based light-activated nanozymes for environmentally friendly colorimetric detection of GSH but also provides a smart strategy for improving nanozyme activity.

2.
Talanta ; 235: 122789, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34517647

RESUMO

In this work, core-shell structured Ti4+-immobilized magnetic covalent organic frameworks (denoted as Fe3O4@TAPTDHTA-Ti4+ composites) were prepared for enhanced phosphopeptide enrichment by one-pot synthesis of COFs shell with inherent bifunctional groups on Fe3O4 NPs and further Ti4+ immobilization. The widely distributed bifunctional groups could provide abundant chelating sites for Ti4+ immobilizing. Combining with the high specific surface area and mesoporous structure, the Fe3O4@TAPTDHTA-Ti4+ composites exhibited excellent enrichment efficiency for phosphopeptides, such as low detection limit (0.05 fmol µL-1), high selectivity (1:5000 of molar ratio of ß-casein/bovine serum albumin (BSA) tryptic digests), high adsorption capacity (62.9 µg mg-1) and strong size-exclusive effect (1:250:250 of molar ratio of ß-casein tryptic digest/ß-casein/BSA). In addition, this method was general for immobilizing other metal ions (Zr4+ and Fe3+). Notably, the Fe3O4@TAPTDHTA-Fe3+ composites exhibited controllable affinity towards mono-phosphopeptides and multi-phosphopeptides. Furthermore, the Fe3O4@TAPTDHTA-Ti4+ composites were successfully applied to selectively capture phosphopeptides from complex biological samples including the tryptic digest of nonfat milk, human serum and human saliva. More significantly, 3333 phosphopeptides derived from 1409 phosphoproteins with 3492 phosphorylation sites were clearly identified from the tryptic digest of HeLa cell lysate. In addition to providing a potential excellent enrichment probe for comprehensive phosphoproteomic analysis, this study also offers a new perspective for the functionalization of COFs.


Assuntos
Estruturas Metalorgânicas , Fosfopeptídeos , Animais , Bovinos , Feminino , Células HeLa , Humanos , Fenômenos Magnéticos , Titânio
3.
J Neurochem ; 2021 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-34338322

RESUMO

Studies have verified that Fragile X mental retardation protein (FMRP), an RNA-binding protein, plays a potential role in the pathogenesis of formalin- and (RS)-3,5-dihydroxyphenylglycine-induced abnormal pain sensations. However, the role of FMRP in inflammatory pain has not been reported. Here, we showed an increase in FMRP expression in the spinal dorsal horn (SDH) in a rat model of inflammatory pain induced by complete Freund's adjuvant (CFA). Double immunofluorescence staining revealed that FMRP was mainly expressed in spinal neurons and colocalized with proinflammatory cytokines [tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)]. After consecutive intrathecal injection of fragile X mental retardation 1 small interfering RNA for 3 days post-CFA injection, FMRP expression in the SDH was reduced, and CFA-induced hyperalgesia was decreased. In addition, the CFA-induced increase in spinal TNF-α and IL-6 production was significantly suppressed by intrathecal administration of fragile X mental retardation 1 small interfering RNA. Together, these results suggest that FMRP regulates TNF-α and IL-6 levels in the SDH and plays an important role in inflammatory pain.

4.
Entropy (Basel) ; 23(7)2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-34356443

RESUMO

Accurate 3D passive tracking of an underwater uncooperative target is of great significance to make use of the sea resources as well as to ensure the safety of our maritime areas. In this paper, a 3D passive underwater uncooperative target tracking problem for a time-varying non-Gaussian environment is studied. Aiming to overcome the low observability drawback inherent in the passive target tracking problem, a distributed passive underwater buoys observing system is considered and the optimal topology of the distributed measurement system is designed based on the nonlinear system observability analysis theory and the Cramer-Rao lower bound (CRLB) analysis method. Then, considering the unknown underwater environment will lead to time-varying non-Gaussian disturbances for both the target's dynamics and the measurements, the robust optimal nonlinear estimator, namely the adaptive particle filter (APF), is proposed. Based on the Bayesian posterior probability and Monte Carlo techniques, the proposed algorithm utilizes the real-time optimal estimation technique to calculate the complex noise online and tackle the underwater uncooperative target tracking problem. Finally, the proposed algorithm is tested by simulated data and comprehensive comparisons along with detailed discussions that are made to demonstrate the effectiveness of the proposed APF.

5.
Entropy (Basel) ; 23(7)2021 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-34356448

RESUMO

The bearing-only tracking of an underwater uncooperative target can protect maritime territories and allows for the utilization of sea resources. Considering the influences of an unknown underwater environment, this work aimed to estimate 2-D locations and velocities of an underwater target with uncertain underwater disturbances. In this paper, an adaptive two-step bearing-only underwater uncooperative target tracking filter (ATSF) for uncertain underwater disturbances is proposed. Considering the nonlinearities of the target's kinematics and the bearing-only measurements, in addition to the uncertain noise caused by an unknown underwater environment, the proposed ATSF consists of two major components, namely, an online noise estimator and a robust extended two-step filter. First, using a modified Sage-Husa online noise estimator, the uncertain process and measurement noise are estimated at each tracking step. Then, by adopting an extended state and by using a robust negative matrix-correcting method in conjunction with a regularized Newton-Gauss iteration scheme, the current state of the underwater uncooperative target is estimated. Finally, the proposed ATSF was tested via simulations of a 2-D underwater uncooperative target tracking scenario. The Monte Carlo simulation results demonstrated the reliability and accuracy of the proposed ATSF in bearing-only underwater uncooperative tracking missions.

6.
Chem Commun (Camb) ; 57(60): 7362-7365, 2021 Jul 27.
Artigo em Inglês | MEDLINE | ID: mdl-34196343

RESUMO

A facile strategy was introduced for room-temperature controllable synthesis of hierarchically flower-like hollow COFs (FHF-COFs). Furthermore, the universality for synthesis of the HFH-COFs was validated by altering the building units. Inspired by the unique morphology, extremely large surface area and good chemical stability, HFH-COFs could serve as an attractive adsorption probe by loading with gold nanoparticles and be applied to enrichment of brain natriuretic peptide from human serum. This work opens up a whole new approach for controllable synthesis of the HFH-COFs at room temperature and expands the application of COFs as a promising enrichment probe for complex biological samples.


Assuntos
Estruturas Metalorgânicas/química , Peptídeo Natriurético Encefálico/isolamento & purificação , Adsorção , Ouro/química , Humanos , Limite de Detecção , Nanopartículas Metálicas/química , Estruturas Metalorgânicas/síntese química , Peptídeo Natriurético Encefálico/sangue , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
7.
Chemistry ; 2021 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-34319625

RESUMO

Polyoxometalates (POMs)-based materials, with high theoretical capacities and abundant reversible multi-electron redox properties, are considered as promising candidates in lithium-ion storage. However, the poor electronic conductivity, low specific surface area and high solubility in the electrolyte limited their practical applications. Herein, a double-shelled hollow PMo12 -SiO2 @N-C nanofiber (PMo12 -SiO2 @N-C, where PMo12 is [PMo12 O40 ]3- , N-C is nitrogen-doped carbon) was fabricated for the first time by combining coaxial electrospinning technique, thermal treatment and electrostatic adsorption. As an anode material for LIBs, the PMo12 -SiO2 @N-C delivered an excellent specific capacity of 1641 mA h g-1 after 1000 cycles under 2 A g-1 . The excellent electrochemical performance benefited from the unique double-shelled hollow structure of the material, in which the outermost N-C shell cannot only hinder the agglomeration of PMo12 , but also improve its electronic conductivity. The SiO2 inner shell can efficiently avoid the loss of active components. The hollow structure can buffer the volume expansion and accelerate Li+ diffusion during lithiation/delithiation process. Moreover, PMo12 can greatly reduce charge-resistance and facilitate electron transfer of the entire composites, as evidenced by the EIS kinetics study and lithium-ion diffusion analysis. This work paves the way for the fabrication of novel POM-based LIBs anode materials with excellent lithium storage performance.

8.
Biomed Res Int ; 2021: 5368987, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34307653

RESUMO

In the current study, our goal was to obtain a robust model to predict the speed of sound in biodiesel. For this purpose, an extensive databank has been extracted from previously published papers. Then, a Support Vector Machine (SVM) has been optimized by Grey Wolf Optimization (GWO) method to analyze these data and determine the correlation between speed of sound in biodiesel and its related properties including pressure, temperature, molecular weight, and normal melting point. The results were very satisfactory because the values of statistical parameters R 2 and RMSE were obtained 1 and 1.4024, respectively. Here, this is the first time that the sensitivity analysis is used to estimate this target value. This analysis shows that the pressure widely affects the output values with relevancy factor 87.92. Also, our proposed method is highly accurate than other machine learning methods used in papers employed for this objective.


Assuntos
Biocombustíveis , Som , Máquina de Vetores de Suporte , Modelos Teóricos , Análise de Regressão
9.
Analyst ; 146(9): 2991-2997, 2021 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-33949450

RESUMO

A spherical thiol-functionalized covalent organic framework (COF-SH) was designed via a facile thiol-yne click reaction of a alkynyl-terminated COF and pentaerythritol tetra(3-mercaptopropionate). The COF-SH was explored as a new adsorbent for the selective enrichment of Hg2+. The as-prepared COF-SH exhibited a uniform mesoporous structure, a high abundance of binding sites, and good chemical stability, which endow it with great performance for the adsorption of Hg2+ and its corresponding maximum adsorption capacity was up to 617.3 mg g-1. Furthermore, the adsorption behavior of Hg2+ on the COF-SH wasin good agreement with the Langmuir and pseudo-second-order models. The influences of adsorbent dosage, pH, selectivity, and reusability of the COF-SH on Hg2+ adsorption were also investigated. Besides this, the COF-SH showed high selectivity towards Hg2+ even in the presence of a high concentration of K+, Na+, Ca2+, Mg2+ and Zn2+ metal ions. Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), the corresponding limit of detection (LOD) of Hg2+ was determined at very low concentrations of 80 pg mL-1 (equal to 396 amoL µL-1). In addition, the COF-SH was successfully applied to rapidly enrich and sensitively detect Hg2+ in industrial sewage, with recoveries in the range of 101.8-103.4%, demonstrating the promising potential of COF-SH as an effective adsorbent for use in environmental sample pretreatment.

10.
Trends Plant Sci ; 26(9): 885-897, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-33867269

RESUMO

Nitric oxide (NO) sensing is an ancient trait enabled by hemoproteins harboring a highly conserved Heme-Nitric oxide/OXygen (H-NOX) domain that operates throughout bacteria, fungi, and animal kingdoms including in humans, but that has long thought to be absent in plants. Recently, H-NOX-containing plant hemoproteins mediating crucial NO-dependent responses such as stomatal closure and pollen tube guidance have been reported. There are indications that the detection method that led to these discoveries will uncover many more heme-based NO sensors that operate as regulatory sites in complex proteins. Their characterizations will in turn offer a much more complete picture of plant NO responses at both the molecular and systems level.


Assuntos
Hemeproteínas , Óxido Nítrico , Fenômenos Fisiológicos Vegetais , Heme , Oxigênio
11.
Mikrochim Acta ; 188(3): 91, 2021 02 18.
Artigo em Inglês | MEDLINE | ID: mdl-33598812

RESUMO

Magnetic covalent organic frameworks (Fe3O4@TPPCl4) were synthesized via a one-pot process in which magnetic nanoparticles (Fe3O4@MNP) served as a magnetic core and 2,4,6-trihydroxy-1,3,5-benzenetricarbaldehyde (TP) and 2,2',5,5'-tetrachlorobenzidine (PCl4) as two building blocks to form a shell. The as-prepared Fe3O4@TPPCl4 nanoparticles have superior features, including large surface area (186.5 m2 g-1), high porosity, strong magnetic responsiveness (42.6 emu g-1), high chlorine content, and outstanding thermal stability, which make them an ideal adsorbent for highly selective enrichment of polychlorinated naphthalenes (PCNs). Combining with atmospheric pressure gas chromatography tandem mass spectrometry (APGC-MS/MS), a simple analytical method of Fe3O4@TPPCl4-based magnetic solid-phase extraction (MSPE)-APGC-MS/MS was developed, which exhibited good linearity (r ≥ 0.9991) for eight PCNs in the concentration range 0.1-100 ng L-1. Moreover, low detection limits (0.005-0.325 ng L-1), high enrichment factors (46.62-81.97-fold), and good relative standard deviations (RSDs) of inter-day (n = 3, 1.64 to 7.44%) and day-to-day (n = 3, 2.62 to 8.23%) were achieved. This method was successfully applied to the selective enrichment of PCNs in fine particulate matter (PM)2.5 samples, and ultra-trace PCNs were found in the range 1.56-3.75 ng kg-1 with satisfactory recoveries (93.11-105.81%). The successful application demonstrated the great potential of Fe3O4@TPPCl4 nanoparticles as an adsorbent for enrichment of halogenated compounds. Schematic presented one-pot synthesis of magnetic covalent organic framework nanocomposites (Fe3O4@TPPCl4) and their application in the selective enrichment of PCNs from PM2.5 prior to APGC-MS/MS analysis.

12.
J Obstet Gynaecol Res ; 47(2): 698-704, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33274550

RESUMO

AIM: Previous studies indicated that excessive screen time was associated with hypertension in children and adolescents. In our hospital, pregnant women tended to spend a lot of time on electronic devices like smartphones. We aimed to explore the relationship between the screen time and hypertensive disorders of pregnancy (HDP). METHODS: A case-control study was conducted from November 2019 to May 2020. A total of 160 women with HDP and 197 healthy controls who gave birth to children in the same hospital were recruited and information was recorded by questionnaires. Multivariate analysis was conducted to assess the effect of screen time on HDP. RESULTS: The results showed that, exposure to electronic screen before nocturnal sleep in cases was significantly longer than that in controls (P = 0.011, odds ratio = 1.50). Smartphones (and/or tablet computers) used only for entertainment also significantly increased the susceptibility to HDP (P < 0.001, odds ratio = 2.84). Other related factors were following: work experience during pregnancy (P = 0.034, odds ratio = 0.53), history of diabetes mellitus (P = 0.013, odds ratio = 2.55), history of family hypertension (P < 0.001, odds ratio = 3.81), body mass index of pre-pregnancy (>25 kg/m2 ) (P < 0.001, odds ratio = 6.16). CONCLUSION: In conclusion, long exposure to electronic screen before nocturnal sleep and the smartphones usage for only entertainment may be associated with the susceptibility to HDP.


Assuntos
Hipertensão Induzida pela Gravidez , Adolescente , Índice de Massa Corporal , Estudos de Casos e Controles , Criança , Eletrônica , Feminino , Humanos , Hipertensão Induzida pela Gravidez/epidemiologia , Hipertensão Induzida pela Gravidez/etiologia , Gravidez , Fatores de Risco , Sono
14.
J Acoust Soc Am ; 148(3): 1425, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33003878

RESUMO

Superdirectivity can yield large directivity using a small array aperture, but it suffers from high sensitivity to random errors. In a previously proposed model, robust Nth-order superdirectivity was obtained by combining low-order superdirectivity sub-solutions with orders from 0 to N. However, the orders N were exclusively positive integers, which may lead to an over-improved robustness with an excessively degraded directivity. This paper presents an arbitrary-order superdirectivity model of circular sensor arrays, which can achieve a more flexible compromise between directivity and robustness than the previous model. A coefficient η is weighted at the (N + 1)th-order entry of the optimal weighting vector and the new weighting vector will consist of the 0th- to Nth-order and the weighted (N + 1)th-order entries. The beampattern, the directivity factor, and the error sensitivity function can then be expressed as functions of the coefficient η. Moreover, broadband superdirective beampatterns with a desired directivity factor or error sensitivity function can be readily synthesized using the closed-form solutions derived from the arbitrary-order superdirectivity model. Simulations and experimental results show that the proposed model can easily synthesize superdirective beampatterns with arbitrary orders, and it is more flexible than the previous integer-order model. It is also demonstrated that the performance of the proposed method is similar to that of the optimization-based method, but it uses a simple closed-form weighting vector rather than a numerical algorithm.

15.
BMC Cancer ; 20(1): 1015, 2020 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-33081731

RESUMO

BACKGROUND: Immune-related genes is closely related to the occurrence and prognosis of head and neck squamous cell carcinoma (HNSCC). At the same time, immune-related genes have great potential as prognostic markers in many types of cancer. The prognosis of HNSCC is still poor currently, and it may be effective to predict the clinical outcome of HNSCC by immunogenic analysis. METHODS: RNASeq and clinical follow-up information were downloaded from The Cancer Genome Atlas (TCGA), the MINiML format GSE65858 chip expression data was downloaded from NCBI, and immune-related genes was downloaded from the InnateDB database. Immune-related genes in 519 HNSC patients were integrated from TCGA dataset. By using multivariate COX analysis and Lasso regression, robust immune-related gene pairs (IRGPs) that predict clinical outcomes of HNSCC were identified. Finally, a risk prognostic model related to immune gene pair was established and verified by clinical features, test sets and GEO external validation set. RESULTS: A total of 699 IRGPs were significantly correlated with the prognosis of HNSCC patients. Fourteen robust IRGPs were finally obtained by Lasso regression and a prognostic risk prediction model was constructed. Risk score of each sample were calculated based on Risk models and divided into the high-risk group (Risk-H) and low Risk group (Risk-L). Risk models were able to stratify the risk in patients with TNM Stage, Age, gender, and smoking history, and the AUC > 0.65 in training set and test set, shows that 14-IRGPs signature in patients with HNSCC has excellent classification performance. In addition, 14-IRGPs had the highest average C index compared with the prognostic characteristics and T, N, and Age of the 3 previously reported HNSCC. CONCLUSION: This study constructed 14-IRGPs as a novel prognostic marker for predicting survival in HNSCC patients.


Assuntos
Biomarcadores Tumorais/genética , Biologia Computacional/métodos , Perfilação da Expressão Gênica/métodos , Neoplasias de Cabeça e Pescoço/genética , Imunidade Inata , Carcinoma de Células Escamosas de Cabeça e Pescoço/genética , Bases de Dados Genéticas , Feminino , Regulação Neoplásica da Expressão Gênica , Redes Reguladoras de Genes , Neoplasias de Cabeça e Pescoço/imunologia , Neoplasias de Cabeça e Pescoço/patologia , Humanos , Masculino , Estadiamento de Neoplasias , Prognóstico , Análise de Sequência de RNA , Carcinoma de Células Escamosas de Cabeça e Pescoço/imunologia , Carcinoma de Células Escamosas de Cabeça e Pescoço/patologia , Análise de Sobrevida
16.
J Pain Res ; 13: 1461-1470, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32606912

RESUMO

Objective: To explore the effect of genetic polymorphisms of cytokines on the dosage of sufentanil for patient-controlled intravenous analgesia (PCIA) after radical lung cancer surgery. Methods: A total of 100 patients, aged 18 years and above, with ASA grade Ⅰ-Ⅱ and body mass index (BMI) 18.5 to 30, and who were scheduled for radical lung cancer surgery under total intravenous anaesthesia with PCIA of sufentanil from September 2015 to March 2016, were selected. DNA was collected from peripheral blood samples before surgery, and the iMLDRTM multiple single-nucleotide polymorphism typing kit was used to detect 16 related single-nucleotide polymorphism (SNP) sites of interleukin-1A (IL-1A), interleukin-1ß (IL-1ß), interleukin-1RN (IL-1RN), interleukin-6 (IL-6), C-X-C motif chemokine ligand 8 (CXCL8), interleukin-10 (IL-10), tumour necrosis factor (TNF), nuclear factor kappa-B1 (NFκB1), REL (REL proto-oncogene, NF-kB subunit), and nuclear factor kappa-B inhibitor alpha (NFκBIA). The general characteristics of patients, surgery and anaesthesia data, postoperative resting VAS pain scores, postoperative opioid dosages of sufentanil for PCIA and opioid-related adverse events were recorded. The effects of the examined genetic polymorphisms of the cytokines on the dosage of sufentanil were analysed. Results: Eight of 100 patients withdrew for various reasons, and, eventually, 92 patients were included. The patients' resting visual analogue scale (VAS) scores at 24 h, 48 h, and 72 h after surgery were 2.3 ± 1.2, 2.0 ± 0.9, and 1.9 ± 1.0, respectively. The total amounts of sufentanil used were 34.7 ± 10.5 µg, 65.2 ± 13.7 µg, and 94.7 ± 11.6 µg, respectively. We found that the TT genotype of NFκBIA rs696 had higher PCIA sufentanil dosages than the CC genotype and the CT genotype at 48-72 h postoperation (p=0.023, p=0.025, respectively). Conclusion: The genetic polymorphisms of the cytokine NFκBIA rs696 might affect the dosage of sufentanil for PCIA after radical lung cancer surgery. The specific mechanism needs further study.

17.
J Oral Maxillofac Surg ; 78(10): 1780.e1-1780.e14, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32615095

RESUMO

PURPOSE: PER2 gene expression is downregulated in oral squamous cell carcinoma (OSCC) and may have a pivotal role in tumor suppression. However, the biological function and mechanism of action of PER2 in OSCC remain unclear. In this study, the biological functions and anticancer mechanisms of PER2 in OSCC were investigated. MATERIALS AND METHODS: Both stably overexpressed and silenced PER2 OSCC cells were established as an experimental group; empty vector lentivirus and scramble short hairpin RNA lentivirus transfected-cells, as negative control groups; and untreated OSCC cells, as a blank group. Cell proliferation, apoptosis, and glycolysis potential assays were conducted. In addition, the expression of phosphatidylinositol 3-kinase (PI3K), protein kinase B (AKT), phosphorylation of protein kinase B, hexokinase 2 (HK2), pyruvate kinase M (PKM2), and lactate dehydrogenase A (LDHA) was quantified by real-time quantitative polymerase chain reaction and Western blotting. Rescue experiments were performed by the addition of AKT activators in the overexpressed cell line and by the addition of glycolysis inhibitor in the silenced cell line. These findings were verified in vivo using stably transfected OSCC cells overexpressing PER2 implanted in nude mice. RESULTS: PER2 overexpression significantly inhibited OSCC cell proliferation and glycolysis, promoted cell apoptosis, and reduced the expression of PI3K, phosphorylation of protein kinase B, HK2, PKM2, and LDHA. The converse was observed in PER2-silenced OSCC cells. After the addition of AKT activator to cultures of PER2-overexpressed OSCC cells, reduced glucose uptake, lactic acid production, and cell proliferation, combined with increased apoptosis, were substantially reversed. In addition, after the addition of HK2 inhibitor to PER2-silenced OSCC cells to inhibit glycolysis, the reduction in apoptosis and increased proliferation were significantly countermanded. Tumorigenesis experiments in vivo also confirmed that PER2 overexpression suppressed OSCC growth and decreased the expression of HK2, PKM2, and LDHA. CONCLUSIONS: PER2 heightened glycolysis via the PI3K/AKT pathway, heightened cell proliferation and inhibited apoptosis via glycolysis, thereby promoting the development of OSCC.


Assuntos
Carcinoma de Células Escamosas , Neoplasias Bucais , Animais , Apoptose , Carcinoma de Células Escamosas/genética , Linhagem Celular Tumoral , Proliferação de Células , Regulação para Baixo , Glicólise , Camundongos , Camundongos Nus , Neoplasias Bucais/genética , Proteínas Circadianas Period/genética , Proteínas Circadianas Period/metabolismo , Fosfatidilinositol 3-Quinase , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais
18.
Mol Pain ; 16: 1744806920928619, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32496847

RESUMO

Chronic pain has detrimental effects on one's quality of life. However, its treatment options are very limited, and its underlying pathogenesis remains unclear. Recent research has suggested that fragile X mental retardation protein is involved in the development of chronic pain, making it a potential target for prevention and treatment. The current review of literature will examine the function of fragile X mental retardation protein and its associated pathways, through which we hope to gain insight into how fragile X mental retardation protein may contribute to nociceptive sensitization and chronic pain.


Assuntos
Dor Crônica/metabolismo , Proteína do X Frágil de Retardo Mental/metabolismo , Animais , Proteína do X Frágil de Retardo Mental/química , Humanos , Canais Iônicos/metabolismo , Neurônios/metabolismo , Neurônios/patologia , Transdução de Sinais , Serina-Treonina Quinases TOR/metabolismo
19.
J Acoust Soc Am ; 147(4): EL314, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-32359269

RESUMO

In deep-sea source localization, some of the existing methods only estimate the source range, while the others produce large errors in distance estimation when estimating both the range and depth. Here, a convolutional neural network-based method with high accuracy is introduced, in which the source localization problem is solved as a regression problem. The proposed neural network is trained by a normalized acoustic matrix and used to predict the source position. Experimental data from the western Pacific indicate that this method performs satisfactorily: the mean absolute percentage error of the range is 2.10%, while that of the depth is 3.08%.

20.
FASEB J ; 34(5): 6871-6887, 2020 05.
Artigo em Inglês | MEDLINE | ID: mdl-32248623

RESUMO

This study used constitutive CD226 gene knockout (KO) mice as a model to investigate the functions and mechanisms of CD226 in megakaryocyte (MK) maturation and platelet activation. Although CD226 deficiency did not cause MK polyploidization or platelet granule abnormalities, increased MK counts were detected in the femora bone marrow (BM) and spleen of CD226 KO mice. Particularly, CD226 KO mice have a more extensive membrane system in MKs and platelets than wild-type (WT) mice. We also demonstrated that CD226 KO mice displayed increased platelet counts, shortened bleeding time, and enhanced platelet aggregation. CD226 KO platelets had an increased mature platelet ratio compared to the control platelets. In addition, the observed reduction in bleeding time may be due to decreased nitric oxide (NO) production in the platelets. Platelet-specific CD226-deficient mice showed similar increased MK counts, shortened bleeding time, enhanced platelet aggregation, and decreased NO production in platelets. Furthermore, we performed middle cerebral artery occlusion-reperfusion surgery on WT and CD226 KO mice to explore the potential effect of CD226 on acute ischemia-reperfusion injury; the results revealed that CD226 deficiency led to significantly increased infarct area. Thus, CD226 is a promising candidate for the treatment of thrombotic disorders.


Assuntos
Antígenos de Diferenciação de Linfócitos T/sangue , Megacariócitos/citologia , Megacariócitos/fisiologia , Ativação Plaquetária/fisiologia , Animais , Antígenos de Diferenciação de Linfócitos T/genética , Plaquetas/fisiologia , Plaquetas/ultraestrutura , Isquemia Encefálica/sangue , Isquemia Encefálica/genética , Isquemia Encefálica/patologia , Modelos Animais de Doenças , Feminino , Integrina beta3/sangue , Masculino , Megacariócitos/ultraestrutura , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Microscopia Eletrônica de Transmissão , Ativação Plaquetária/genética , Adesividade Plaquetária/genética , Adesividade Plaquetária/fisiologia , Agregação Plaquetária/genética , Agregação Plaquetária/fisiologia , Contagem de Plaquetas , Trombopoese/genética , Trombopoese/fisiologia
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