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1.
J Org Chem ; 84(24): 16171-16182, 2019 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-31774681

RESUMO

Density functional theory (DFT) calculations were performed to investigate the photosensitizer-free visible-light-mediated gold-catalyzed cis-difunctionalization of alkynes with aryl diazonium salts. The detailed reaction mechanism is established, and the observed regio- and chemoselectivities are rationalized. The results are compared to those of the rhodium-catalyzed cis-difunctionalization of alkynes. It is indicated that the excitation of the aryl diazonium salt initiates the photocatalytic cycle, and the following single-electron transfer between the Au(I) catalyst and the excited aryl diazonium salt affords the key aryl radical. Both gold- and rhodium-catalyzed reactions involve two major steps: alkyne insertion into the M-N or M-C bond (M = Au, Rh), and C-C or C-N reductive elimination from the M(III) center. The cis-difunctionalized product can be obtained by the trimethylsilyl (TMS)-substituted alkyne through the gold catalysis or by the Ph-substituted alkyne through the rhodium catalysis. The catalyst-dependent reactivity switch of TMS- and Ph-substituted alkynes is attributed to the catalyst-induced shift of the rate-determining step.

2.
J Org Chem ; 84(15): 9705-9713, 2019 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-31246456

RESUMO

This work presents a DFT study on the mechanism and origin of catalyst-controlled divergent reactivity in the synthesis of benzo-heterocycles from o-alkynylbenzamides by Au(I)/Pt(IV) catalysis. The results indicate that the transformations proceed via a nucleophilic cyclization process. In the Au(I) catalysis, the preferred O-attack mode mainly originates from the symmetry match in the dominant bond-forming interaction between the lone-pair orbital of carbonyl-O and the in-plane alkyne π* orbital, and the electronic property of the ligand controls the O-5-exo-dig/O-6-endo-dig selectivity. The preference for the N-attack mode in Pt(IV) catalysis is attributed to the stronger coordinate capability of carbonyl-O than amino-N in the substrate to PtCl4, and the regioselective N-6-endo-dig or N-5-exo-dig cyclization depends on the stronger electrostatic interaction between the amino-N and alkynyl-Cß atoms. The theoretical results provide a fundamental understanding of why and how gold and platinum complexes catalyze the cyclization of o-alkynylbenzamides with different chemo- and regioselectivities.

3.
J Org Chem ; 84(2): 579-588, 2019 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-30394741

RESUMO

The mechanisms and chemoselectivities on the Au(I)-catalyzed intermolecular condensation between homopropargyl alcohols and terminal alkynes were investigated by performing DFT calculations. The reaction was indicated to involve three stages: transformation of the homopropargyl alcohol (R1) via intramolecular cyclization to the cyclic vinyl ether (R1'), formation of the C-2-arylalkynyl cyclic ether (P1) via hydroalkynylation of R1' with phenylacetylene (R2), and conversion from P1 to 2,3-dihydro-oxepine (P2). The results revealed the origin of the reaction divergence and rationalized the experimental observations that a 1:3 reactant stoichiometric ratio affords P1 as the major product, whereas the 1:1.1 ratio results in P2 in high yield. The reactant stoichiometric ratio-controlled divergent reactivity is attributed to different catalytic activities of the gold catalyst toward different reaction stages. In the 1:3 situation, the excess R2 induces the Au catalyst toward its dimerization and/or hydration, inhibiting the conversion of P1 to P2 and resulting in product P1. Without excess R2, the Au catalysis follows a general cascade reaction, leading to product P2. Theoretical results described a general strategy controlling the reaction divergence by a different reactant stoichiometric ratio. This strategy may be enlightening for chemists who are exploring various synthesis methods with high chemo-, regio-, and enantioselectivities.

4.
Chemistry ; 24(53): 14119-14126, 2018 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-30052273

RESUMO

Recently, a photosensitizer-free visible-light-mediated gold-catalyzed 1,2-difunctionalization of alkynes has been developed. However, mechanistic aspects of this unconventional photocatalytic reaction remain largely obscure. With the aid of density functional theory (DFT) and time-dependent (TD)DFT calculations, we mimicked the photosensitizer-free visible-light-mediated gold-catalyzed 1,2-difunctionalization of 1-phenyl-1-hexyne and focused on two fundamental questions: how does photoredox catalysis occur without assistance of an exogenous photosensitizer under visible light irradiation, and what is the detailed mechanism of the gold-catalyzed 1,2-difunctionalization of alkynes? The results reveal the dual role of the gold(I) complex in light-harvesting and catalysis, where a charge-transfer (CT) complex formed by the association of gold(I) catalyst with PhN2 BF4 acts as a photosensitizer, which can undergo an electronic transition between the gold(I) moiety and PhN2 BF4 of the CT complex into an excited electronic state and afford a charge-transfer exciplex. The oxidative quenching of the exciplex generates the gold(II) species and diazobenzene radical. The subsequent catalytic cycle proceeds via two parallel pathways, involving the radical addition to gold(II) and gold(I) centers, respectively, and in these two pathways the reductive elimination of gold(III) species is identified as the rate-determining step of the whole reaction. The present study could provide a new understanding for exogenous-photosensitizer-free visible-light-mediated gold-catalyzed processes.

5.
Int J Mol Med ; 42(3): 1765, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29845220

RESUMO

Subsequently to the publication of this article, the authors have realized that the address affiliation for the corresponding author, Chengheng Hu, and the authors Longyun Peng and Xinxue Liao appeared incorrectly. These authors' affiliation information should have appeared as follows (the corrected address affiliation is featured in bold): XIAO KE1,2*, JINGFU CHEN3*, LONGYUN PENG4, WEI ZHANG5, YIYING YANG5, XINXUE LIAO4, LIQIU MO6, RUIXIAN GUO7, JIANQIANG FENG6, CHENGHENG HU4 and RUQIONG NIE2 1Department of Cardiology, Shenzhen Sun Yat­sen Cardiovascular Hospital, Shenzhen; 2Department of Cardiology, Sun Yat­sen Memorial Hospital, Sun Yat­sen University, Guangzhou, Guangdong; 3Department of Cardiovascular Medicine and Dongguan Cardiovascular Institute, The Third People's Hospital of Dongguan City, Dongguan; 4Department of Cardiology and Key Laboratory on Assisted Circulation, Ministry of Health, The First Affiliated Hospital, Sun Yat­sen University; 5Department of Cardiovasology and Cardiac Care Unit (CCU), Huangpu Division of The First Affiliated Hospital, Sun Yat­sen University; 6Department of Anesthesiology, Huangpu Division of The First Affiliated Hospital, Sun Yat­sen University; 7Department of Physiology, Zhongshan School of Medicine, Sun Yat­sen University, Guangzhou, Guangdong, P.R. China *Contributed equally In addition, the address for correspondence in the correspondence box should have appeared as follows: Correspondence to: Professor Chengheng Hu, Department of Cardiology and Key Laboratory on Assisted Circulation, Ministry of Health, The First Affiliated Hospital, Sun Yat­sen University, Guangdong, 58 Zhongshan 2rd Road, Guangzhou 510080, P.R. China E­mail: huchengheng138@163.com The authors regret this error in the affiliations, and apologize for any inconvenience caused. [the original article was published in the International Journal of Molecular Medicine 39: 1001­1010, 2017; DOI: 10.3892/ijmm.2017.2891].

6.
Int J Mol Med ; 42(2): 1199, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-29749426

RESUMO

Subsequently to the publication of this article, the authors have realized that an address affiliation associated with certain of the authors had been omitted. The authors' affiliation information should have appeared as follows (the omitted address affiliation is featured in bold): Yi­Ying Yang1,2*, Xiu­Ting Sun1,2*, Zheng­Xun Li1,2, Wei­Yan Chen3, Xiang Wang4, Mei­Ling Liang5, Hui Shi1,2, Zhi­Sheng Yang1,2 and Wu­Tao Zeng1,2 1Department of Cardiology, The First Affiliated Hospital, Sun Yat­Sen University; 2Key Laboratory on Assisted Circulation, Ministry of Health, Guangzhou, Guangdong 510080; 3Intensive Care Unit, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510260; 4Department of Cardiology, Laiwu City People's Hospital, Laiwu, Shandong 27110; 5Department of Cardiology, Sun Yat­Sen Cardiovascular Hospital, Shenzhen, Guangdong 518020, P.R. China *Contributed equally. The authors regret this error in the affiliations, and apologize for any inconvenience caused. [the original article was published in the International Journal of Molecular Medicine 41: 1283­1292, 2018; DOI: 10.3892/ijmm.2017.3322].

7.
J Org Chem ; 83(5): 2763-2772, 2018 03 02.
Artigo em Inglês | MEDLINE | ID: mdl-29431999

RESUMO

This work aims at understanding the mechanism and regioselectivity in ligand-controlled gold-catalyzed divergent intramolecular hydroarylation of alkynes reported by Jiang et al. ( J. Am. Chem. Soc. 2016 , 138 , 5218 - 5221 ). Focusing on a representative alkyne, N-propargyl-N-tosylaniline, we conducted a detailed computational study on the ortho- and para-position hydroarylation of the alkyne catalyzed by gold(I) catalysts with different ligands. Both the ortho- and para-position hydroarylation reactions are found to follow a similar three-stage mechanism: electrophilic cyclization, proton loss, and protiodeauration. The initial electrophilic cyclization was identified as the rate- and regiochemistry-determining step. With the flexible electron-deficient phosphite ligand, the ortho-position cyclization is identified as the energetically more favorable pathway, while with the rigid electron-abundant phosphine (Xphos) ligand, the dominant pathway turns to the para-position cyclization. The theoretical results are in good agreement with the experimental observations. The π-π interaction between alkynyl phenyl and the directing acylamino group are found to be mainly responsible for the observed ortho-selectivity, while a combination of favorable noncovalent CH···π interaction and steric repulsion between Xphos ligand and alkynyl group contributes to the observed exclusive para-selectivity. The present calculations provide deeper insight into the mechanism and origin of regioselectivity of the title reaction.

8.
Biofouling ; 34(1): 1-14, 2018 01.
Artigo em Inglês | MEDLINE | ID: mdl-29210309

RESUMO

Colanic acid (CA) is a group I extracellular polysaccharide (EPS) that contributes to resistance against adverse environments in many members of the Enterobacteriaceae. In the present study, a genetic locus EPSC putatively involved in CA biosynthesis was identified in Vibrio alginolyticus ZJ-51, which undergoes colony morphology variation between translucent/smooth (ZJ-T) and opaque/rugose (ZJ-O). EPSC in ZJ-T carries 21 ORFs and resembles the CA cluster of Escherichia coli K-12. The deletion of EPSC led to decreased EPS and biofilm formation in both genetic backgrounds but no alternation of lipopolysaccharide. The loss of this locus also changed the colony morphology of ZJ-O on the 2216E plate and reduced the motility of ZJ-T. Compared with ZJ-T, ZJ-O lacks a 10-kb fragment (epsT) in EPSC containing homologs of wecA, wzx and wzy that are essential for O-antigen synthesis. However, the deletion or overexpression of epsT resulted in no change of colony morphology, biofilm formation or EPS production. This study reported at the first time a genetic locus EPSC that may be involved in colanic acid synthesis in V. alginolyticus ZJ-51, and found that it was related to EPS biosynthesis, biofilm formation, colony morphology and motility, which may shed light on the environmental adaptation of the vibrios.


Assuntos
Regulação Bacteriana da Expressão Gênica , Genes Bacterianos , Polissacarídeos/biossíntese , Vibrio alginolyticus/genética , Vibrio alginolyticus/metabolismo , Biofilmes/crescimento & desenvolvimento , Deleção de Genes , Variação Genética , Polissacarídeos Bacterianos/biossíntese
9.
Int J Mol Med ; 41(3): 1283-1292, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29286068

RESUMO

Angiotensin-(1-7) [Ang-(1-7)], a heptapeptide mainly generated from cleavage of AngⅠ and AngⅡ, possesses physiological and pharmacological properties, including anti­inflammatory and antidiabetic properties. Activation of the phosphoinositide 3-kinase and protein kinase B (PI3K̸Akt) signaling pathway has been confirmed to participate in cardioprotection against hyperglycaemia-induced injury. The aim of the present study was to test the hypothesis that Ang-(1-7) protects H9c2 cardiomyoblast cells against high glucose (HG)-induced injury by activating the PI3K̸Akt pathway. To examine this hypothesis, H9c2 cells were treated with 35 mmol/l (mM) glucose (HG) for 24 h to establish a HG-induced cardiomyocyte injury model. The cells were co-treated with 1 µmol/l (µM) Ang-(1-7) and 35 mM glucose. The findings of the present study demonstrated that exposure of H9c2 cells to HG for 24 h markedly induced injury, as evidenced by an increase in the percentage of apoptotic cells, generation of reactive oxygen species and level of inflammatory cytokines, as well as a decline in cell viability and mitochondrial luminosity. These injuries were significantly attenuated by co-treatment of the cells with Ang-(1-7) and HG. In addition, PI3K̸Akt phosphorylation was suppressed by HG treatment, but this effect was abolished when the H9c2 cells were co-treated with Ang-(1-7) and HG. Furthermore, the cardioprotection of Ang-(1-7) against HG-induced injury in H9c2 cardiomyoblasts was highly attenuated in the presence of either D-Ala7-Ang-(1-7) (A-779, an antagonist of the Mas receptor) or LY294002 (an inhibitor of PI3K̸Akt). In conclusion, the present study provided new evidence that Ang-(1-7) protects H9c2 cardiomyoblasts against HG-induced injury by activating the PI3K̸Akt signaling pathway.


Assuntos
Angiotensina I/farmacologia , Cardiotônicos/farmacologia , Hiperglicemia/patologia , Miócitos Cardíacos/enzimologia , Miócitos Cardíacos/patologia , Fragmentos de Peptídeos/farmacologia , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais/efeitos dos fármacos , Animais , Apoptose/efeitos dos fármacos , Caspases/metabolismo , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Citoproteção/efeitos dos fármacos , Glucose/toxicidade , Inflamação/patologia , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Miócitos Cardíacos/efeitos dos fármacos , Fosforilação/efeitos dos fármacos , Inibidores de Proteínas Quinases/farmacologia , Ratos , Espécies Reativas de Oxigênio/metabolismo
10.
Mol Med Rep ; 17(1): 1461-1468, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29257199

RESUMO

The transplantation of mesenchymal stem cells (MSCs) has been a reported method for alleviating atherosclerosis (AS). Because the availability of bone marrow­derived MSCs (BM­MSCs) is limited, the authors used this study to explore the use of a new type of MSC, human induced pluripotent stem cell­derived MSCs (iPSC­MSCs), to evaluate whether these cells could alleviate AS. iPSC­MSCs were intravenously administered to ApoE knock out mice fed on a high­fat diet (HFD) for 12 weeks. It was reported that systematically administering iPSC­MSCs clearly reduced the size of plaques. In addition, the numbers of macrophages and lipids in plaques were lower in the HFD + iPSC­MSCs group than in the HFD group. Furthermore, iPSC­MSCs attenuated AS­associated inflammation by decreasing the levels of inflammatory cytokines, such as tumor necrosis factor­α and interleukin­6, in serum. In addition, the expression of Notch1 was higher in the HFD group, and injecting iPSC­MSCs reversed this effect. In conclusion, the current study provides the first evidence indicating that iPSC­MSCs may be a new optional MSC­based strategy for treating AS.


Assuntos
Aterosclerose/terapia , Células-Tronco Pluripotentes Induzidas/citologia , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais/citologia , Animais , Aterosclerose/sangue , Aterosclerose/complicações , Aterosclerose/imunologia , Células Cultivadas , Citocinas/sangue , Citocinas/imunologia , Humanos , Células-Tronco Pluripotentes Induzidas/imunologia , Inflamação/sangue , Inflamação/complicações , Inflamação/imunologia , Inflamação/terapia , Masculino , Transplante de Células-Tronco Mesenquimais/métodos , Células-Tronco Mesenquimais/imunologia , Camundongos , Camundongos Endogâmicos C57BL
11.
Int J Pediatr Otorhinolaryngol ; 103: 51-54, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29224765

RESUMO

BACKGROUND: There is no standardized scheme for preoperative evaluation of adenoid hypertrophy or a consensus on surgical indications for adenoidectomy in children with otitis media with effusion (OME), especially for young children intolerant to nasal endoscopic assessment. The aim of this study was to evaluate the efficacy and reliability of acoustic rhinometry (AR) in evaluating benefits from adenoidectomy in children with OME. METHOD: Children with OME who were scheduled for surgical intervention were reviewed and AR tests performed preoperatively and postoperatively. The patients were divided into two groups based on the surgical strategy (Group I: tympanostomy tube placement alone; Group II: tympanostomy tube placement plus adenoidectomy). Correlation and regression analyses were performed to assess the relationship between findings of AR and nasal endoscopy. AR parameters including minimal nasal cross-sectional area (MCA), and nasopharyngeal volume (NPV), as well as scores of subjective symptoms were obtained to evaluate the utility of AR pre- and post-surgery. RESULTS: Sixty-five children aged 4-10 years who met the inclusion criteria were included. No significant differences in gender or age distribution were observed between Group I and Group II. MCA, as well as NPV significantly decreased in Group II when compared with Group I (p = 0.000). A significant inverse correlation was observed between NPV and choanal obstruction ratio in both groups I (r = -0.625, p < 0.001) and II (r = -0.570, p < 0.001). A significant difference between preoperative and postoperative NPV and subjective symptom scores was observed in group II after adenoidectomy (p = 0.000). CONCLUSION: AR parameters showed a good clinical correlation with findings of nasal endoscopy and thus may be useful for evaluating candidacy for surgical adenoidectomy among children with OME, especially in whom preoperative nasal endoscopic examination is not feasible. Additionally, AR can reveal the changes occurring within the nasopharyngeal passage before and after adenoidectomy.


Assuntos
Adenoidectomia/métodos , Tonsila Faríngea/patologia , Ventilação da Orelha Média/métodos , Otite Média com Derrame/fisiopatologia , Rinometria Acústica/métodos , Criança , Pré-Escolar , Endoscopia , Feminino , Humanos , Hipertrofia/cirurgia , Masculino , Cavidade Nasal/fisiopatologia , Otite Média com Derrame/cirurgia , Reprodutibilidade dos Testes , Estudos Retrospectivos
12.
J Infect ; 75(6): 499-510, 2017 12.
Artigo em Inglês | MEDLINE | ID: mdl-28941629

RESUMO

OBJECTIVE: We validated the accuracy of host selected signature gene set using unstimulated whole blood (WB), and peripheral blood mononuclear cells (PBMC) in the diagnosis of tuberculosis (TB). METHODS: The unstimulated WB and PBMC from 1417 individuals with active pulmonary TB patients, other lung diseases and healthy participants were analyzed using real time polymerase chain reaction (RT-PCR). RESULTS: The WB cohort test demonstrates that the combination of GBP5 and KLF2 can differentiate active TB versus HC with sensitivity and specificity of 77.8% and 87.1%, respectively; but most importantly active TB versus OD with sensitivity and specificity of 96.1% and 85.2%, respectively. Again during treatment course, the TB score of GBP5 and KLF2, analytes secretion and clinical parameters were found to be associated in disease progression. In the PBMC cohort test, we found that the only and best discriminatory combination was GBP5, DUSP3 and KLF2 inthe active TB versus HC with a sensitivity and specificity of 76.4% and 85.9%, respectively. CONCLUSIONS: Our study reveals that GBP5 and KLF2 may be useful as a diagnostic tool for active TB, also the two-gene set may serve as surrogate biomarkers for monitoring TB therapy.


Assuntos
Proteínas de Ligação ao GTP/genética , Fatores de Transcrição Kruppel-Like/genética , Pneumopatias/diagnóstico , Tuberculose Pulmonar/diagnóstico , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores/sangue , Estudos de Coortes , Diagnóstico Diferencial , Fosfatase 3 de Especificidade Dupla/genética , Feminino , Perfilação da Expressão Gênica/métodos , Humanos , Leucócitos Mononucleares , Masculino , Pessoa de Meia-Idade , RNA , Estatísticas não Paramétricas , Adulto Jovem
13.
Mar Genomics ; 35: 23-26, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28395865

RESUMO

Branched-chain amino acids (BCAAs) play important roles in nitrogen metabolism. However, little is known about the metabolism of BCAAs in the fish pathogen Vibrio alginolyticus. In this study, the global gene expression patterns of V. alginolyticus ZJ-T cultured in M63 minimal medium supplemented with ammonium sulfate or with three BCAAs (isoleucine, leucine and valine) as nitrogen source were evaluated by transcriptome analysis. The results revealed that 311 genes are up-regulated (|log2(Fold Change)|>1), which are involved in the pathways of flagellar assembly, bacterial chemotaxis and oxidative phosphorylation etc, and meanwhile 251 genes are down-regulated, which are involved in the pathways of BCAAs biosynthesis, selenocompound metabolism and C5-branced dibasic acid metabolism etc. This study contributes to the understanding of the BCAAs metabolism in the Vibrios.


Assuntos
Aminoácidos de Cadeia Ramificada/metabolismo , Transcriptoma , Vibrio alginolyticus/metabolismo , Perfilação da Expressão Gênica
14.
Int J Mol Med ; 39(4): 1001-1010, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28204829

RESUMO

It has been reported that exogenous hydrogen sulfide (H2S) protects against high glucose (HG)-induced cardiac injury and has a modulatory effect on heat shock protein (HSP) and Akt, which play a cardioprotective role. In this study, we examined whether the HSP90/Akt pathway contributes to the protective effects of exogenous H2S against HG-induced injury to H9c2 cardiac cells. Our results revealed that the exposure of H9c2 cardiac cells to 35 mM glucose (HG) for 1 to 24 h decreased the expression of HSP90 and markedly reduced the expression level of phosphorylated (p)-Akt in a time-dependent manner. Co-exposure of the cells to HG and geldanamycin (GA; an inhibitor of HSP90) aggravated the inhibition of the p-Akt expression level by HG. Of note, treatment of the cells with 400 µM NaHS (a donor of H2S) for 30 min prior to exposure to HG significantly attenuated the HG-induced decrease in the expression levels of both HSP90 and p-Akt, along with inhibition of HG-induced cell injury, as indicated by the increase in cell viability and superoxide dismutase (SOD) activity, and by a decrease in the number of apoptotic cells, reactive oxygen species (ROS) generation, as well as by the decreased dissipation of mitochondrial membrance potential (MMP). Importantly, treatment of the cells with GA or LY294002 (an inhibitor of Akt) prior to exposure to NaHS and HG considerably blocked the cardioprotective effects of NaHS against the HG-induced injury mentioned above. On the whole, the findings of this study demonstrate that the inhibition of the HSP90/Akt pathway may be an important mechanism responsible for HG-induced cardiomyocyte injury. We also provide novel evidence that exogenous H2S protects H9c2 cells against HG-induced injury by activating the HSP90/Akt pathway.


Assuntos
Cardiotônicos/farmacologia , Glucose/efeitos adversos , Proteínas de Choque Térmico HSP90/metabolismo , Sulfeto de Hidrogênio/farmacologia , Miócitos Cardíacos/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais/efeitos dos fármacos , Animais , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Cromonas/farmacologia , Glucose/farmacologia , Morfolinas/farmacologia , Miócitos Cardíacos/patologia , Proteínas Proto-Oncogênicas c-akt/antagonistas & inibidores , Ratos , Espécies Reativas de Oxigênio/metabolismo
15.
J Autism Dev Disord ; 47(3): 615-625, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-27981390

RESUMO

Previous research has demonstrated abnormal trust and deception behaviors in children with Autism Spectrum Disorders (ASD), and we aimed to examine whether these abnormalities were primarily due to their specific deficits in social learning. We tested 42 high-functioning children with ASD and 38 age- and ability-matched typically developing (TD) children in trust and deception tasks and a novel condition with reduced social components. Results indicated that while TD children improved their performance with more social components, children with ASD lacked this additional performance gain, though they performed similarly as TD children in the condition with reduced social components. Our findings highlight that deficits of ASD in trust and deception are primarily associated with failure of use of social cues.


Assuntos
Transtorno do Espectro Autista/psicologia , Decepção , Aprendizado Social , Confiança , Estudos de Casos e Controles , Criança , Pré-Escolar , Feminino , Humanos , Masculino
16.
Genome Announc ; 4(5)2016 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-27587824

RESUMO

Vibrio alginolyticus is a ubiquitous Gram-negative bacterium which is normally distributed in the coastal and estuarine environments. It has been suggested to be an opportunistic pathogen to both marine animals and humans, Here, the completed genome sequence of V. alginolyticus ZJ-T was determined by Illumina high-throughput sequencing.

17.
PLoS One ; 11(9): e0163689, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27685640

RESUMO

Hfq is a global regulator that is involved in environmental adaptation of bacteria and in pathogenicity. To gain insight into the role of Hfq in Vibrio alginolyticus, an hfq deletion mutant was constructed in V. alginolyticus ZJ-T strain and phenotypically characterized. Deletion of hfq led to an alteration of colony morphology and reduced extracellular polysaccharide production, a general impairment of growth in both rich medium and minimal media with different carbon sources or amino acids, enhanced sensitivity to oxidative stress and to several antibiotics. Furthermore, a differential transcriptomic analysis showed significant changes of transcript abundance for 306 protein coding genes, with 179 genes being up regulated and 127 down-regulated. Several of these changes could be related to the observed phenotypes of the mutant. Transcriptomic data also provided evidence for the induction of the extracytoplasmic stress response in absence of Hfq. Altogether, these findings point to broad regulatory functions for Hfq in V. alginolyticus cells, likely to underlie an important role in pathogenicity.

18.
Eur J Clin Pharmacol ; 72(11): 1327-1334, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27488389

RESUMO

PURPOSE: The aim of this study was to investigate whether any of the single-nucleotide polymorphisms (SNPs) in the POR gene were significantly associated with CYP activity and expression, and could contribute to the total variability in stable warfarin maintenance doses in Han Chinese. METHODS: A total of 408 patients treated at the First Affiliated Hospital of Sun Yat-Sen University were eligible for the study and had attained a stable warfarin maintenance dose at the start of the investigation. Demographics, warfarin maintenance doses, and concomitant medications were documented. Genomic DNA was extracted from peripheral blood samples and genotyped for ten SNPs (CYP 2C9*2 and *3, CYP4F2 rs2108622, VKORC1 -1639C>T, and potential POR genes of rs10239977, rs3815455, rs41301394, rs56256515, rs1057868, and rs2286823) using the Sequenom MassARRAY genotyping system. RESULTS: A predictive model of warfarin maintenance dose was established and indicated that age, gender, body surface area, aspirin use, CYP2C9*3, CYP4F2 rs2108622, VKORC1 -1639C>T, and POR*37 831-35C>T accounted for 42.4 % of dose variance in patients undergoing anticoagulant treatment. The contribution of POR*37 831-35C>T to warfarin dose variation was only 3.9 %. CONCLUSIONS: For the first time, the SNP POR*37 831-35C>T was confirmed as a minor but statistically significant factor associated with interindividual variation in warfarin maintenance dose in Han Chinese. The POR*37 gene polymorphism should be considered in future algorithms for faster and more reliable achievement of stable warfarin maintenance doses.


Assuntos
Anticoagulantes/administração & dosagem , Grupo com Ancestrais do Continente Asiático/genética , Sistema Enzimático do Citocromo P-450/genética , Modelos Biológicos , Varfarina/administração & dosagem , Adulto , Idoso , Idoso de 80 Anos ou mais , Anticoagulantes/uso terapêutico , Relação Dose-Resposta a Droga , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Polimorfismo de Nucleotídeo Único , Varfarina/uso terapêutico , Adulto Jovem
19.
Pestic Biochem Physiol ; 127: 15-20, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26821653

RESUMO

10-Hydroxycamptothecin (HCPT), a plant alkaloid isolated from Camptotheca acuminate, is known as a planted-derived insecticide, however, the specific mechanism in insect cells is still unclear. In this study, we treated the ovarian cell line of the silkworm, BmN-SWU1, with different HCPT doses for durations ranging from 0 to 72h. The apoptosis morphology was evident after 72h of incubation and included cell protuberance, concentrated cytoplasm and apoptotic bodies. We observed DNA fragmentation and cell apoptosis after HCPT treatment. The disruption of mitochondrial distribution, activation of the intracellular mitochondrial permeability transition pore, and release of cytochrome c during HCPT-induced apoptosis in dose and time-dependent manner indicate the involvement of mitochondria in BmN-SWU1 cells. Caspase-9 and -3 activities increased gradually with the duration of incubation time. In conclusion, HCPT has a significant effect to initiate the intrinsic mitochondrial pathway in silkworm cells, providing a theoretical basis for better application of plant-derived insecticide in pest control.


Assuntos
Camptotecina/análogos & derivados , Mitocôndrias/efeitos dos fármacos , Animais , Apoptose , Bombyx , Camptotecina/farmacologia , Linhagem Celular , Feminino , Ovário/citologia
20.
Chemistry ; 20(45): 14736-43, 2014 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-25234357

RESUMO

A newly prepared [(ppy)2 Ir(dcbpy)](+) ⋅PF6 (-) (ppy: 2-phenylpyridyl; dcbpy: 4,4'-dicarboxy-2,2'-bipyridyl) and gold nanoparticle functionalized mesoporous silica nanoparticle (Au/Ir-MSN) is reported. Based on the binding between concanavalin A (Con A) and mannose, the novel nanoparticle was applied to an ultrasensitive electrochemiluminescence (ECL) in situ cytosensing strategy and the dynamic evaluation of cell-surface carbohydrate expression. The ECL activity of the presented Con A@Au/Ir-MSN nanoprobe was greatly enhanced by employing a functionalized nanoparticle and graphene nanomaterial with an increased surface area and simultaneously improved electron-transfer efficiency at the electrode interface. Under optimal conditions, the sandwich-type ECL cytosensor showed a linear response to K562 cells at concentrations ranging from 1.0×10(2) to 1.0×10(6)  cells mL(-1) and realized a low detection limit of a single cell. The proposed method could also be successfully used for monitoring the dynamic variation of carbohydrate expression in cancer cells in response to external stimulation by an inhibitor.


Assuntos
Carboidratos/biossíntese , Complexos de Coordenação/química , Técnicas Eletroquímicas/métodos , Irídio/química , Medições Luminescentes/métodos , Nanopartículas Metálicas/química , Carboidratos/análise , Humanos , Células K562
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