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Pediatr Dent ; 43(4): 290-295, 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-34467846


Purpose: The purpose of this study was to evaluate the treatment outcomes of multisurface caries in primary molars treated with intracoronal restorations versus stainless steel crowns (SSCs) through a retrospective split-mouth study. Methods: Dental records were screened for patients who had treatment of one primary molar with a multisurface restoration and one primary molar with an SSC. Teeth were followed until a loss to follow-up, exfoliation, or failure. Results: A total of 988 primary molars were evaluated, with a mean follow-up time of 22 months. The survival probabilities for: SSCs were 95.5 percent at one year of service and 92.8 percent at two years of service; and for intracoronal restorations were 92.0 percent at one year of service and 80.0 percent at two years of service. Overall survival analysis showed SSCs to be significantly more successful than restorations (P<0.001), particularly in children treated at ages four years and younger (P<0.001). No statistically significant difference (P=0.10) was found for children treated at ages five years and older. Conclusions: Stainless steel crowns have a higher survival probability versus restorations for multisurface caries. In children ages four years and younger, more aggressive treatment of multi-surface caries with SSCs should be considered, as conservative treatment leads to an increased need for retreatment.

Cárie Dentária , Aço Inoxidável , Criança , Pré-Escolar , Coroas , Cárie Dentária/terapia , Falha de Restauração Dentária , Restauração Dentária Permanente , Humanos , Dente Molar , Estudos Retrospectivos , Dente Decíduo
Oncol Res ; 19(7): 323-33, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21936402


Newcastle disease virus (NDV) and variants of this virus have oncolytic properties and are potential anticancer agents. The objective of this study was to compare the effect of NDV strain D90 and strain D93 isolated from natural sources on human non-small cell lung cancer (NSCLC) cell line A549. We determined the 50% embryo infective dose (EID50) and 50% tissue culture infective dose (TCID50) of the NDV strains. The MTT assay was used to evaluate the effects of NDV strains on cell viability. We determined the expression of Annexin V and Bcl-2 proteins in NDV-infected cells. Light microscopy and electron microscopy indicated that the D90 strain significantly altered cell morphology and reduced cell viability, while strain D93 had negligible effects. Neither strain had a significant effect on normal cultured fetal liver cells. We used acridine orange staining to show that strain D90 (but not strain D93) induced nuclear fragmentation of A549 cells. An Annexin V-based apoptosis assay indicated that strain D90 (but not strain D93) caused significant apoptosis of A549 cells. Moreover, strain D90 (but not strain D93) significantly repressed the expression of Bcl-2 (an antiapoptotic protein) in A549 cells. Taken together, our results indicate that NDV strain D90 (but not strain D93) had no significant effect on normal cultured cells, but induced apoptosis of cultured NSCLC cells via a caspase-dependent pathway. These results suggest that NDV strain D90 has potential as an anticancer agent.

Neoplasias Pulmonares/terapia , Vírus da Doença de Newcastle , Terapia Viral Oncolítica , Apoptose , Linhagem Celular Tumoral , Sobrevivência Celular , Humanos , Neoplasias Pulmonares/patologia , Neoplasias Pulmonares/ultraestrutura , Microscopia Eletrônica , Proteínas Proto-Oncogênicas c-bcl-2/análise