Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 152
Filtrar
1.
Int J Mol Sci ; 22(18)2021 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-34575868

RESUMO

Temperature is an important factor that largely affects the patterns of shoot branching in plants. However, the effect and mechanism of temperature on axillary bud development in chrysanthemum remains poorly defined. The purpose of the present study is to investigate the effect of high temperature on the axillary bud growth and the mechanism of axillary bud formation in chrysanthemum. Decapitation experiments combined with the transcriptome analysis were designed. Results showed that the axillary bud length was significantly inhibited by high temperature. Decapitation of primary shoot (primary decapitation) resulted in slower growth of axillary buds (secondary buds) under 35 °C. However, secondary decapitation resulted in complete arrest of tertiary buds at high temperature. These results demonstrated that high temperature not only inhibited axillary bud formation but also retarded bud outgrowth in chrysanthemum. Comparative transcriptome suggested differentially expressed gene sets and identified important modules associated with bud formation. This research helped to elucidate the regulatory mechanism of high temperature on axillary bud growth, especially bud formation in chrysanthemum. Meanwhile, in-depth studies of this imperative temperature signaling can offer the likelihood of vital future applications in chrysanthemum breeding and branching control.

2.
Adv Mater ; : e2104002, 2021 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-34499382

RESUMO

Photoresponsive materials have drawn much attention and are widely applied in daily life for their reversible changes in luminous color or appearance color under light irradiation. In this work, a new photoresponsive system based on triarylamine derivatives is developed. With the changed aryl substituents, adjustable photoresponsive properties, including photoactivated phosphorescence and photochromism after being dispersed into the poly(methyl methacrylate) (PMMA) matrix, are demonstrated. According to the theoretical calculations and experimental data, the competition between the formations of triplet excitons and cationic radicals under photoirradiation should be the main reason for their different photoresponsive properties. Excitingly, the applications of rewritable photopatterning, anticounterfeiting, information encryption, and decryption are realized conveniently, in addition to the successful model of sunglasses to protect eyes away from ultraviolet radiation and strong light in the sunlight. These studies present a simple and efficient design strategy for the development of photoresponsive materials on modulating the phosphorescence and photochromic property.

3.
J Hazard Mater ; 420: 126556, 2021 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-34280723

RESUMO

Imidacloprid (IMI), as the most-consumed pesticide, has posed a severe threat to the water ecosystem due to its recalcitrance and inefficient elimination in the traditional wastewater treatment. Herein, a heterogeneous electro-Fenton (EF) system coupled with 3D-MoO2-porous biochar (PBC) cocatalytic electrodes, abbreviated as 3D-MPE-EF, is initially applied to promote the elimination of IMI in the agrochemical wastewater from pesticide production. The elimination rate of IMI by 3D-MPE-EF system is 18.15 times higher than that by traditional EF system at pH 7.0. The utilization of 3D-MoO2-PBC electrodes sufficiently compensates for inherent deficiencies of traditional EF system. The circular utilization of Fe is also addressed by 3D-MoO2-PBC cocatalytic electrodes to reduce the consumption of Fe2+ and the deposition of iron mud. Through comparison, MoO2 is considered the most appropriate cocatalyst in terms of the reutilization of Fe and degradation of IMI. Eight mechanisms are identified in the degradation pathways of IMI by UPLC-Q-TOF-MS. The ecotoxicities of IMI are remarkably attenuated in the 3D-MPE-EF system. This study provides insights into the roles of 3D-MoO2-PBC cocatalytic electrodes in the enhanced elimination of IMI in heterogeneous EF system.

4.
Int J Mol Sci ; 22(11)2021 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-34198826

RESUMO

The aryl hydrocarbon receptor (AHR) is a cytosolic receptor which is involved in diverse cellular events in humans. The most well-characterized function of AHR is its ability to upregulate gene transcription after exposure to its ligands, such as environmental toxicants, dietary antioxidants, drugs, and endogenous ligands. The cellular content of AHR is partly controlled by its degradation via the ubiquitin-proteasome system and the lysosome-dependent autophagy. We used human cervical cancer (HeLa) cells to investigate how AHR undergoes protein degradation and how its activity is modulated. Since the glycogen synthase kinase 3 beta (GSK3ß)-mediated phosphorylation can trigger protein degradation and substrates of GSK3ß contain stretches of serine/threonine residues which can be found in AHR, we examined whether degradation and activity of AHR can be controlled by GSK3ß. We observed that AHR undergoes the GSK3ß-dependent, LC3-mediated lysosomal degradation without ligand treatment. The AHR can be phosphorylated in a GSK3ß-dependent manner at three putative sites (S436/S440/S444, S689/S693/T697, and S723/S727/T731), which leads to lysosomal degradation of the AHR protein. Inhibition of the GSK3ß activity suppresses the ligand-activated transcription of an AHR target gene in HeLa, human liver cancer (Hep3B), and human breast cancer (MCF-7) cells. Collectively, our findings support that phosphorylation of AHR by GSK3ß is essential for the optimal activation of its target gene transcription and this phosphorylation may partake as an "off" switch by subjecting the receptor to lysosomal degradation.


Assuntos
Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética , Glicogênio Sintase Quinase 3 beta/genética , Neoplasias/genética , Receptores de Hidrocarboneto Arílico/genética , Transcrição Genética , Autofagia/genética , Células HeLa , Humanos , Lisossomos/genética , Células MCF-7 , Neoplasias/patologia , Complexo de Endopeptidases do Proteassoma/genética , Proteólise , Ubiquitina/genética
5.
Artigo em Inglês | MEDLINE | ID: mdl-34014080

RESUMO

In this work, several carbon-decorated Na3V2(PO4)3 materials (NVP@C-750/800/850) are successfully fabricated using a sol-gel approach and subsequent heat treatment. When NVP@C-800 is used as a cathode, it shows an ultralong cycle life (2000 cycles) at a high rate of 10C, which is superior to the other two electrodes and those of reported NVP@C cathodes in the literature. The excellent results of NVP@C-800 are attributed to its nanostructure and the well-defined conductive carbon layer. The symmetric sodium (Na)-ion battery (SIB) with NVP@C-800 as both a cathode and an anode shows a high capacity at 40 mA g-1 with a voltage plateau of about 1.79 V and energy density of 113 W h kg-1, revealing that NVP@C is of great application prospect.

6.
Virus Res ; 300: 198445, 2021 07 15.
Artigo em Inglês | MEDLINE | ID: mdl-33961897

RESUMO

The emergency SARS-CoV-2, a member of severe acute respiratory syndrome-related coronaviruses (SARSr-CoV), is still greatly harming the health of mankind. SARS-CoV-2-specific monoclonal antibodies (MAbs), which can identify SARS-CoV-2 from common human coronaviruses, are considered to extensively apply to developing rapid and reliable antigen assays. In this study we generated a rabbit MAb (RAb) detecting SARS-CoV-2 nucleocapsid protein (NP), which has cross-reaction with SARS-CoV-1 NP, but not with NPs of MERS and common human CoVs (OC43, NL63, 229E, and HKU1). With truncated NP fragments and synthesized peptides, the linear epitope detected by RAb was mapped in peptide N4-8, 393-407 amino acid residue (TLLPAADLDDFSKQL) of SARS-CoV-2 NP. This epitope N4-8 was highly conserved in SARSr-CoVs, including SARS-CoV-2, SARS-CoV-1, and bat CoV RaTG13 strain. However, the corresponding peptide of bat SARSr-CoV BtKY72 strain could not be recognized by RAb, which indicates amino acid D399 may be critical for N4-8 epitope detected by RAb. The present study will be conducive to developing reliable diagnosis for SARS-CoV-2 and gaining insights into the function of the SARS-CoV-2 N protein.


Assuntos
Anticorpos Monoclonais/imunologia , Teste Sorológico para COVID-19/métodos , COVID-19/diagnóstico , Proteínas do Nucleocapsídeo de Coronavírus/imunologia , SARS-CoV-2 , Mapeamento de Epitopos , Humanos , SARS-CoV-2/imunologia , SARS-CoV-2/isolamento & purificação , Sensibilidade e Especificidade
7.
Molecules ; 26(5)2021 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-33806413

RESUMO

MsrB1 used to be named selenoprotein R, for it was first identified as a selenocysteine containing protein by searching for the selenocysteine insert sequence (SECIS) in the human genome. Later, it was found that MsrB1 is homologous to PilB in Neisseria gonorrhoeae, which is a methionine sulfoxide reductase (Msr), specifically reducing L-methionine sulfoxide (L-Met-O) in proteins. In humans and mice, four members constitute the Msr family, which are MsrA, MsrB1, MsrB2, and MsrB3. MsrA can reduce free or protein-containing L-Met-O (S), whereas MsrBs can only function on the L-Met-O (R) epimer in proteins. Though there are isomerases existent that could transfer L-Met-O (S) to L-Met-O (R) and vice-versa, the loss of Msr individually results in different phenotypes in mice models. These observations indicate that the function of one Msr cannot be totally complemented by another. Among the mammalian Msrs, MsrB1 is the only selenocysteine-containing protein, and we recently found that loss of MsrB1 perturbs the synaptic plasticity in mice, along with the astrogliosis in their brains. In this review, we summarized the effects resulting from Msr deficiency and the bioactivity of selenium in the central nervous system, especially those that we learned from the MsrB1 knockout mouse model. We hope it will be helpful in better understanding how the trace element selenium participates in the reduction of L-Met-O and becomes involved in neurobiology.


Assuntos
Sistema Nervoso Central/patologia , Gliose/patologia , Metionina Sulfóxido Redutases/fisiologia , Plasticidade Neuronal , Selênio/metabolismo , Animais , Sistema Nervoso Central/metabolismo , Gliose/etiologia , Gliose/metabolismo , Humanos , Camundongos , Camundongos Knockout
8.
Neurotherapeutics ; 18(2): 962-978, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33723752

RESUMO

Glucagon-like peptide-1 (GLP-1) receptor stimulation ameliorates parkinsonian motor and non-motor deficits in both experimental animals and patients; however, the disease-modifying mechanisms of GLP-1 receptor activation have remained unknown. The present study investigated whether exendin-4 (a GLP-1 analogue) can rescue motor deficits and exert disease-modifying effects in a parkinsonian rat model of α-synucleinopathy. This model was established by unilaterally injecting AAV-9-A53T-α-synuclein into the right substantia nigra pars compacta, followed by 4 or 8 weeks of twice-daily intraperitoneal injections of exendin-4 (5 µg/kg/day) starting at 2 weeks after AAV-9-A53T-α-synuclein injections. Positron emission tomography/computed tomography (PET/CT) scanning and immunostaining established that treatment with exendin-4 attenuated tyrosine-hydroxylase-positive neuronal loss and terminal denervation and mitigated the decrease in expression of vesicular monoamine transporter 2 within the nigrostriatal dopaminergic systems of rats injected with AAV-9-A53T-α-synuclein. It also mitigated the parkinsonian motor deficits assessed in behavioral tests. Furthermore, through both in vivo and in vitro models of Parkinson's disease, we showed that exendin-4 promoted autophagy and mediated degradation of pathological α-synuclein, the effects of which were counteracted by 3-methyladenine or chloroquine, the autophagic inhibitors. Additionally, exendin-4 attenuated dysregulation of the PI3K/Akt/mTOR pathway in rats injected with AAV-9-A53T-α-synuclein. Taken together, our results demonstrate that exendin-4 treatment relieved behavioral deficits, dopaminergic degeneration, and pathological α-synuclein aggregation in a parkinsonian rat model of α-synucleinopathy and that these effects were mediated by enhanced autophagy via inhibiting the PI3K/Akt/mTOR pathway. In light of the safety and tolerance of exendin-4 administration, our results suggest that exendin-4 may represent a promising disease-modifying treatment for Parkinson's disease.

9.
Cancer Res ; 81(15): 4145-4154, 2021 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-33727230

RESUMO

Poor penetration of mAbs in solid tumors is explained, in part, by the binding site barrier hypothesis. Following extravasation, mAbs rapidly bind cellular antigens, leading to the observation that, at subsaturating doses, therapeutic antibody in solid tumors localizes around tumor vasculature. Here we report a unique strategy to overcome the binding site barrier through transient competitive inhibition of antibody-antigen binding. The anti-trastuzumab single domain antibody 1HE was identified through in vitro binding assays as a model inhibitor. Coadministration of 1HE did not alter the plasma pharmacokinetics of trastuzumab or ado-trastuzumab emtansine (T-DM1) in vivo. Administration of 1HE alone was rapidly eliminated with a terminal plasma half-life of 1.2 hours, while coadministrations of 1HE with trastuzumab had a terminal half-life of 56 hours. In mice harboring SKOV3 xenografts, coadministration of 1HE with trastuzumab led to significant increases in both penetration of trastuzumab from vasculature and the percentage of tumor area that stained positive for trastuzumab. 1HE coadministered with a single dose of T-DM1 to NCI-N87 xenograft-bearing mice significantly enhanced T-DM1 efficacy, increasing median survival. These results support the hypothesis that transient competitive inhibition can improve therapeutic antibody distribution in solid tumors and enhance antibody efficacy. SIGNIFICANCE: This study describes the development of a transient competitive inhibition strategy that enhances the tumor penetration and efficacy of anticancer antibodies.See related commentary by van Dongen, p. 3956.

10.
Int J Mol Sci ; 22(4)2021 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-33562118

RESUMO

The aryl hydrocarbon receptor (AHR) is a ligand-activated signaling molecule expressed in many cell types, including triple-negative and non-triple-negative breast cancer cells. It affects breast cancer growth and crosstalk with estrogen receptor signaling. Normally, this receptor is degraded shortly after ligand activation via the 26S proteasome. Here, we report that AHR undergoes chaperone-mediated autophagy in MDA-MB-468 triple-negative breast cancer cells. This lysosomal degradation of AHR exhibits the following characteristics: (1) it is triggered by 6 amino-nicotinamide, starvation, and piperazinylpyrimidine compound Q18; (2) it is not observed in non-triple-negative breast cancer cells (MCF-7, T47D, and MDA-MB-361); (3) it can be inhibited by progesterone receptor B but not estrogen receptor alpha; (4) it can be reversed by chloroquine but not MG132; (5) it requires LAMP2A; and (6) it involves AHR-HSC70 and AHR-LAMP2A interactions. The NEKFF sequence localized at amino acid 558 of human AHR appears to be a KFERQ-like motif of chaperone-mediated autophagy, responsible for the LAMP2A-mediated AHR protein degradation.


Assuntos
Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo , Autofagia Mediada por Chaperonas/fisiologia , Proteína 2 de Membrana Associada ao Lisossomo/metabolismo , Proteólise , Receptores de Hidrocarboneto Arílico/metabolismo , Neoplasias de Mama Triplo Negativas/patologia , Sequência de Aminoácidos , Linhagem Celular Tumoral , Cloroquina/farmacologia , Citocromo P-450 CYP1A1/genética , Citocromo P-450 CYP1A1/metabolismo , Receptor alfa de Estrogênio/metabolismo , Humanos , Leupeptinas/farmacologia , Lisossomos/metabolismo , Células MCF-7 , Interferência de RNA , Receptores de Progesterona/genética , Receptores de Progesterona/metabolismo , Transdução de Sinais/fisiologia
11.
New Phytol ; 230(5): 2001-2010, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-33586157

RESUMO

Plants control water-use efficiency (WUE) by regulating water loss and CO2 diffusion through stomata. Variation in stomatal control has been reported among lineages of vascular plants, thus giving rise to the possibility that different lineages may show distinct WUE dynamics in response to water stress. Here, we compared the response of gas exchange to decreasing leaf water potential among four ferns and nine seed plant species exposed to a gradually intensifying water deficit. The data collected were combined with those from 339 phylogenetically diverse species obtained from previous studies. In well-watered angiosperms, the maximum stomatal conductance was high and greater than that required for maximum WUE, but drought stress caused a rapid reduction in stomatal conductance and an increase in WUE in response to elevated concentrations of abscisic acid. However, in ferns, stomata did not open beyond the optimum point corresponding to maximum WUE and actually exhibited a steady WUE in response to dehydration. Thus, seed plants showed improved photosynthetic WUE under water stress. The ability of seed plants to increase WUE could provide them with an advantage over ferns under drought conditions, thereby presumably increasing their fitness under selection pressure by drought.


Assuntos
Gleiquênias , Ácido Abscísico , Desidratação , Secas , Folhas de Planta , Estômatos de Plantas , Sementes , Água
12.
J Nanosci Nanotechnol ; 21(3): 1728-1734, 2021 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-33404439

RESUMO

Local natural persimmons were used as a new precursor to synthesize carbon quantum dots (CQDs) by a brief and environment friendly strategy, hydrothermal method. The as-prepared CQDs were characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, Ultraviolet-visible spectroscopy, as well as fluorescence spectrophotometer. The average diameter of CQDs was 2.5±0.5 nm with spherical shape and exhibited blue fluorescence with the maximum emission wavelength at 438 nm and excitation wavelength at 345 nm. The influences of pH and ionic strength on CQDs fluorescence were evaluated. Moreover, the CQDs were used for determination of Fe3+ by blue fluorescence quenching as the result of the interaction between Fe3+ and -OH, -COOH on the CQDs surfaces. The proposed CQDs displayed high selectivity and sensitivity of Fe3+ in comparison with other metal ions. Therefore, a good linear relationship was established with the Fe3+ concentration in the range of 0.6-400 µM. The correlation coefficient of the calibration curve was 0.996. The detection limit of the method was 0.56 µM. Eventually, the CQDs made from persimmons were used for determination of Fe3+ in actual water sample with satisfactory results and gave recoveries between 95.66% and 99.90%. The relative standard deviation was 1.20%-1.86%. The results demonstrated the potential towards diverse applications of the local persimmons.

13.
Br J Pharmacol ; 178(7): 1524-1540, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33474722

RESUMO

BACKGROUND AND PURPOSE: Disturbed flow induces endothelial dysfunction and contributes to uneven distribution of atherosclerotic plaque. Emerging evidence suggests that harmine, a natural constituent of extracts of Peganum harmala, has potent beneficial activities. Here, we investigated if harmine has an atheroprotective role under disturbed flow and the underlying mechanism. EXPERIMENTAL APPROACH: Mice of ApoE-/- , LDLR-/- , and endothelial cell (EC)-specific overexpression of yes-associated protein (YAP) in ApoE-/- background were fed with a Western diet and given harmine for 4 weeks. Atherosclerotic lesion size, cellular composition, and expression of inflammatory genes in the aortic roots were assessed. HUVECs were treated with oscillatory shear stress (OSS) and harmine and also used for proteomic analysis. KEY RESULTS: Harmine retarded atherogenesis in both ApoE-/- and LDLR-/- mice by inhibiting the endothelial inflammatory response. Mechanistically, harmine blocked OSS-induced YAP nuclear translocation and EC activation by reducing phosphorylation of YAP at Y357. Overexpression of endothelial YAP blunted the beneficial effects of harmine in mice. Proteomic study revealed that protein tyrosine phosphatase non-receptor type 14 (PTPN14) could bind to YAP. Moreover, harmine increased PTPN14 expression by stabilizing its protein level and inhibiting its degradation in proteasomes. PTPN14 knockdown blocked the effects of harmine on YAPY357 and EC activation. Finally, overexpression of PTPN14 mimicked the effects of harmine and ameliorated atherosclerosis, and knockdown of PTPN14 blunted the atheroprotective effects of harmine and accelerated atherosclerosis, in a partial ligation mouse model. CONCLUSION AND IMPLICATIONS: Harmine alleviated OSS-induced EC activation via a PTPN14/YAPY357 pathway and had a potent atheroprotective role.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal , Aterosclerose , Proteínas Tirosina Fosfatases não Receptoras , Proteínas Adaptadoras de Transdução de Sinal/genética , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Animais , Aterosclerose/tratamento farmacológico , Aterosclerose/prevenção & controle , Harmina/farmacologia , Células Endoteliais da Veia Umbilical Humana , Humanos , Camundongos , Camundongos Knockout , Proteômica , Fatores de Transcrição/metabolismo
14.
Am J Otolaryngol ; 42(2): 102896, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33445037

RESUMO

OBJECTIVES: This study aims to propose a novel and effective throat swab collection method for coronavirus disease 2019 (COVID-19). METHODS: The subjects were randomly divided into two groups. The subjects were asked to open their mouth to make "ah" sound (traditional method) or simulate yawn (improved method) for throat swab collection. The usage of tongue depressor, collection time, adverse reactions and subjective discomfort (VAS score) were compared. The collection time, comprehensive indicators of adverse reactions and VAS score were also compared among three collectors. RESULTS: The tongue depressor was less used in the improved group (χ2 = 40.186, P < 0.01). The average collection time of the traditional group was 5.44 ± 2.97 and that of the improved group was 4.00 ± 2.31 (P < 0.01). The subjects in the improved group had fewer and milder adverse reactions. The VAS score of subjects in the improved group was lower than that in the traditional group (P < 0.01). Among different collectors, the collection time, comprehensive indicators of adverse reactions and VAS were the same as the overall trend. CONCLUSION: Simulating yawn is a safer and faster throat swab collection method.


Assuntos
COVID-19/diagnóstico , Faringe/virologia , Manejo de Espécimes/métodos , Bocejo , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Distribuição Aleatória , Fatores de Tempo , Escala Visual Analógica , Adulto Jovem
15.
Mov Disord ; 36(2): 442-448, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-33107659

RESUMO

BACKGROUND: There was a paucity of follow-up studies in the disease progression of early-onset PD patients with Parkin mutations (Parkin-EOPD). Here we conducted a longitudinal study to investigate the progression of motor and cognitive features of Parkin-EOPD patients. METHODS: Genetic analysis was performed via target sequencing and multiplex ligation-dependent probe amplification. Thirty patients carrying homozygous or compound heterozygous Parkin mutations with at least 2 follow-up revisions were investigated as the Parkin-EOPD group. Fifty-two patients with at least 2 follow-up revisions, who did not have any known causative PD mutations, GBA or LRRK2 risk variants, a heterozygous Parkin mutation or 2 Parkin mutations without a segregation test, were defined as the genetically undefined EOPD (GU-EOPD) group. A linear mixed-effect model was implemented to evaluate longitudinal changes in motor symptoms and cognition. RESULTS: At baseline, the Parkin-EOPD group had a lower Unified Parkinson's Disease Rating Scale score (UPDRS-III) (off-medication) than the GU-EOPD group, without significant differences in cognition. A longitudinal study showed the estimated progression rate per year (standard error) of the UPDRS-III score (off-medication) was lower in the Parkin-EOPD group (0.203 [0.3162] points per year) than in the GU-EOPD group (1.056 [0.3001] points per year). The difference in the UPDRS-III score rate between the 2 groups was 0.853 (0.4183) (P = 0.042). The Parkin-EOPD group showed better maintenance of spatial processing ability compared with the GU-EOPD group (P = 0.027). CONCLUSION: Parkin-EOPD patients showed a slower deterioration of motor symptoms and a better spatial processing ability than GU-EOPD patients, which suggests that subtyping according to genetic features can help predict PD progression. © 2020 International Parkinson and Movement Disorder Society.


Assuntos
Doença de Parkinson , Idade de Início , Progressão da Doença , Heterozigoto , Humanos , Estudos Longitudinais , Mutação/genética , Doença de Parkinson/genética , Ubiquitina-Proteína Ligases/genética
16.
Colloids Surf B Biointerfaces ; 198: 111461, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33246779

RESUMO

Nanocrystals, due to high drug loading efficiency, have drawn large attention as nanotechnology to enhance solubility and bioavailability of poorly soluble drugs. However, most nanocrystals still encountered low oral absorption percentage due to its insufficient retention time in the gastrointestinal tract (GI). In this work, silybin (SB) as model drug was fabricated to nanocrystals, and further loaded into a mucoadhesive microsphere to increase the GI retention. Such mucoadhesive microspheres were prepared with a wet media milling technique followed by coagulation and film coating. Nanocrystals and microspheres were thoroughly characterized by diverse complementary techniques. As results, such delivery system displayed an encapsulation efficiency of approximately 100 % and a drug loading capacity of up to 35.41 ± 0.31 %. In addition, mucoadhesiveness test ex vivo conducted with rat intestine showed that film-coated microspheres were retained for more than 1 h. Benefiting from nanocrystals technology, the drug cumulative release percentage of the microspheres was remarkable improved compared to unprocessed one in vitro. Finally, pharmacokinetics studies in rats showed a significant 3-fold increase of drug oral bioavailability compared to unprocessed SB. The current study demonstrates that the developed delivery vehicle can enhance the bioavailability of SB by increasing its dissolution percentage as well as through extending retention time in the GI tract, and achieve high drug loading capacity.


Assuntos
Nanopartículas , Preparações Farmacêuticas , Administração Oral , Animais , Disponibilidade Biológica , Sistemas de Liberação de Medicamentos , Microesferas , Tamanho da Partícula , Ratos , Silibina , Solubilidade
17.
World Neurosurg ; 145: e224-e232, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33059078

RESUMO

OBJECTIVE: An optimized Enhanced Recovery After Surgery (ERAS) program is lacking for adolescent idiopathic scoliosis (AIS). The aim of the present study was to evaluate the impact and feasibility of an optimized ERAS pathway in patients with surgically treated AIS. METHODS: In total, 79 patients with AIS who underwent corrective surgery without 3-column osteotomy were recruited from Xijing Hospital of the Fourth Military Medical University between 2012 and 2018. Forty-four patients were treated according to a traditional protocol and 35 were managed using an optimized ERAS pathway, which was designed and implemented by a multidisciplinary team. The following data were collected and retrospectively analyzed, demographic characteristics, Cobb angle, curve type (Lenke), surgical duration, fusion level, correction rate, estimated blood loss, postoperative hemoglobin level, postoperative pain score, pain relief time, hemovac drainage, drainage removal time, first ambulation time, length of hospital stay, and postoperative complications. RESULTS: There was no significant difference between the traditional and ERAS groups with respect to demographic characteristics, Cobb angle, curve type (Lenke), fusion level, and correction rate. However, the ERAS group had a shorter surgical duration, less blood loss and hemovac drainage, a higher postoperative hemoglobin level, and earlier pain relief, ambulation, and discharge. The rates of postoperative nausea and vomiting were lower in the ERAS group than in the traditional group. CONCLUSIONS: The ERAS pathway is capable of improving the perioperative status of patients with AIS by offering stronger analgesia, faster ambulation, and earlier discharge.


Assuntos
Recuperação Pós-Cirúrgica Melhorada , Escoliose/cirurgia , Adolescente , Estudos de Coortes , Feminino , Humanos , Masculino , Complicações Pós-Operatórias/etiologia , Complicações Pós-Operatórias/prevenção & controle , Estudos Retrospectivos , Fusão Vertebral/efeitos adversos , Fusão Vertebral/métodos
18.
World Neurosurg ; 149: 502-510, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33326856

RESUMO

OBJECTIVE: This work aimed to analyze the value of serum platelet/lymphocyte ratio (PLR), carbohydrate antigen 125 (CA125), and diffusion-weighted imaging (DWI) in the diagnosis of recurrent ovarian cancer. METHODS: Forty-three patients with suspected recurrence of ovarian cancer were deemed as research objects, and 5 healthy people were set as controls. PLR, DWI, and CA125 level before surgery were analyzed. Pearson correlation analysis was implemented to explore the correlation between CA125 and lymphocyte count, platelet count, and PLR. Neuropathic Pain Scale was adopted to analyze the analgesic effect of patients with recurrent ovarian cancer before treatment and 7 days after treatment. Moreover, the quality of life scores of patients with recurrent ovarian cancer were evaluated before treatment and 30 days after treatment. RESULTS: The platelet and PLR values of patients with recurrent ovarian cancer were significantly higher relative to healthy people (P < 0.001), while the lymphocyte count was relatively lower (P = 0.002). There were considerable differences in PLR and CA125 levels in patients with recurrent ovarian cancer before and after treatment (P < 0.001). The single detection of CA125 was significantly better than that of DWI and PLR (P = 0.034). The sensitivity and specificity of CA125 detection alone was 77.3% and 81.8%, respectively, while those of the joint detection of DWI + PLR + CA125 was 92.4% and 79.9%, respectively. PLR was proved to be positively correlated with CA125 before and after treatment (r = 0.687, P < 0.001). Pain scores in all aspects of patients with recurrence of ovarian cancer after treatment were lower than that before treatment (P < 0.001), and the quality of life score was significantly higher than that before treatment (P < 0.001). CONCLUSIONS: CA125 and PLR combined with DWI had the best diagnostic effect for patients with recurrent ovarian cancer. After treatment, the levels of PLR and CA125 were reduced and the quality of life of patients was improved.


Assuntos
Antígeno Ca-125/sangue , Dor do Câncer/diagnóstico , Proteínas de Membrana/sangue , Neuralgia/diagnóstico , Neoplasias Ovarianas/diagnóstico , Imagem de Difusão por Ressonância Magnética , Feminino , Humanos , Contagem de Linfócitos , Recidiva Local de Neoplasia/complicações , Recidiva Local de Neoplasia/diagnóstico , Neuralgia/etiologia , Neoplasias Ovarianas/complicações , Contagem de Plaquetas , Estudos Retrospectivos
19.
Arch Gerontol Geriatr ; 92: 104255, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-32966944

RESUMO

BACKGROUND: Older people present to the emergency department (ED) with distinct patterns and emergency care needs. This study aimed to use comprehensive geriatric assessment (CGA) surveying the patterns of ED visits among older patients and determine frailty associated with the risk of revisits/readmission. METHODS: This prospective study screened 2270 patients aged ≥75 years in the ED from August 2018 to February 2019. All patients underwent CGA. A 3-months follow-up was conducted to observe the hospital courses of admission and revisit/readmission. RESULTS: A total of 270 older patients were enrolled. The independent predictors of admission at initial ED visit were the risk of nutritional deficit and instrumental activities of daily living (IADL). In the admission group, the independent predictors of revisit/readmission were a fall in the past year and mobility difficulties. In the discharge group, the independent predictors of revisit/readmission were frailty and insomnia. Regardless if older patients were either admitted or discharged at the initial ED visit, the independent predictor of revisit/readmission for older patients was frailty. CONCLUSION: Our study showed that frailty was the only independent predictor for revisit/readmission after ED discharge during the 3-month follow up. For ED physicians, malnutrition and IADL were independent predictors in recognizing whether the older patient should be admitted to the hospital. For discharged older ED patients, frailty was the independent predictor for the integration of community services for older patients to decrease the rate of revisit/readmission in 3 months.


Assuntos
Avaliação Geriátrica , Readmissão do Paciente , Atividades Cotidianas , Idoso , Idoso de 80 Anos ou mais , Serviço Hospitalar de Emergência , Seguimentos , Humanos , Alta do Paciente , Estudos Prospectivos
20.
Materials (Basel) ; 13(23)2020 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-33297365

RESUMO

Nanofibrillated cellulose and microfibrillated cellulose are potential raw materials separated from plant fibers with a high aspect ratio and excellent mechanical properties, which can be applied in various fields (packaging, medicine, etc.). They have unique advantages in the preparation of aerogels and foams, and have attracted widespread attention in recent years. Cellulose-based porous materials have good biodegradability and biocompatibility, while high porosity and high specific surface area endow them with strong mechanical properties and liquid retention performance, which can be used in wall construction, sewage treatment and other fields. At present, the preparation method of this material has been widely reported, however, due to various process problems, the actual production has not been realized. In this paper, we summarize the existing technical problems and main solutions; in the meantime, two stable systems and several drying processes are described, and the application potential of cellulose-based porous materials in the future is described, which provides a reference for subsequent research.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...