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1.
Cell Mol Biol (Noisy-le-grand) ; 66(3): 155-158, 2020 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-32538763

RESUMO

Atherosclerosis is the pathological basis of cardiovascular diseases (CVDs) which are the leading cause of death worldwide. The pathogenesis of atherosclerosis itself is complex. Low-density lipoprotein (LDL) oxidation has been shown to increase lipid peroxide levels in arterial wall of atherosclerosis lesion site. Decursin is a coumarin with a range of pharmacological effects. The present study investigated the inhibitory effect of decursin on LDL oxidation, and its protective effect against oxidative damage in human aortic endothelial cells (HAECs). Two models of oxidative damage were used in this study: Cu2+-induced LDL oxidative damage and 2,2'-azobis-2-methyl-propanimidyl, dihydrochloride (AAPH)-induced oxidative damage of HAECs. The inhibitory effect of decursin on LDL oxidation, and its protective effect on oxidative damage of HAECs were determined. The results showed that the level of thiobarbituric acid reactive substances (TBARS) was significantly increased by Cu2+, but was significantly and concentration-dependently reduced after treatment with decursin (p < 0.05). There were only a few viable cells in AAPH-treated group, but treatment with decursin led to significant, time- and concentration-dependent increases in their viability (p < 0.05). The AAPH-induced oxidative damage significantly increased the activity of lactate dehydrogenase (LDH) and level of reactive oxygen species (ROS), but significantly reduced the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) (p < 0.05). However, treatment of HAECs with decursin significantly and concentration-dependently reduced the LDH activity and level of ROS, but significantly increased the activities of SOD and GPx, concentration-dependently (p < 0.05). It also significantly and concentration-dependently reduced apoptosis induced by AAPH (p < 0.05). These results show that decursin effectively inhibits LDL oxidation induced by Cu2+, and protects HAECs from oxidative damage caused by AAPH in vitro via a mechanism involving activation of SOD and GPx.

2.
Med Sci Monit ; 26: e923758, 2020 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-32541641

RESUMO

BACKGROUND The purpose of this study was to investigate factors influencing bleeding in patients with acute coronary syndrome (ACS) who are on aspirin and ticagrelor as dual antiplatelet therapy. MATERIAL AND METHODS This retrospective case-control study included 50 patients with ACS (25 with reported bleeding events and 25 without) on aspirin and ticagrelor. Adenosine diphosphate (ADP)- and arachidonic acid (ACA)-induced platelet aggregation rates were measured using light transmission aggregometry. Single-nucleotide polymorphisms (SNPs) in PEAR1, GP1BA, and GSTP1 were genotyped. RESULTS ACA-induced platelet aggregation rates were obviously lower in patients with bleeding events than in those without (13.28±8.46% vs. 24.93±9.89%, P<0.001). No significant differences in ADP-induced platelet aggregation rates were observed between the 2 groups (16.17±9.74% vs. 16.88±12.69%, P>0.05). Among those with bleeding events and among controls, 70% and 80% had an ACA-induced platelet aggregation rate of 0-18% and 18-50%, respectively. Mutation rates of rs6065 in GP1BA and rs1695, rs4891, and rs8191439 in GSTP1 also differed significantly between the 2 groups. CONCLUSIONS Lower ACA-induced platelet aggregation rates are associated with increased risk of bleeding in patients with ACS who are on aspirin and ticagrelor. An ACA-induced platelet aggregation rate of 18% may be considered the cutoff point for identifying high risk of aspirin-associated bleeding events in patients with ACS. SNP genotyping may also help predict the risk of bleeding in patients with ACS.

3.
Nat Commun ; 11(1): 2559, 2020 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-32444612

RESUMO

Kinetic resolution (KR) of racemic starting materials is a powerful and practical alternative to prepare valuable enantiomerically enriched compounds. A magnesium-catalyzed kinetic resolution based on a designed intramolecular vinylogous Michael reaction is disclosed. Here we show a synergistic catalytic strategy based on the development of chiral ligands. Substrates containing linear allylic ester structures are designed and synthesized to construct key [6.6.5]-tricyclic chiral skeletons via this kinetic resolution process. Detailed mechanistic studies reveal a rational mechanism for the current intramolecular vinylogous KR reaction. The desired direct intramolecular asymmetric vinylogous Michael reaction of linear allylic esters is realized in high efficiency and enantioselectivity with the synergistic catalytic system.

4.
Sci Total Environ ; 728: 138830, 2020 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-32353800

RESUMO

Due to the increasing concerns on environmental pollution, fossil energy shortage, and sustainable development, the recycling of industrial by-products had become a popular practice worldwide. Chemical stabilization of problematic soils with biomass by-product lignin was being considered as one of the viable answers to the consumption of such lignin stockpiles and the reduction of environmental loading. This paper summarized the production and physicochemical properties of by-product lignin collected from paper mills and reviewed the state of the art of this lignin stabilized soils as engineering materials. In addition, the potential focuses requiring further study to promote lignin stabilization technology were expected. The results showed that physicochemical properties of by-product lignin were mainly controlled by plant biomass and production technology, which should be clearly examined before field application. By-product lignin exhibited a satisfactory performance of improving engineering properties of both cohesive soils and noncohesive soils with respect to strength, erosion resistance, and durability. The precipitated cementing materials bonded particles and filled pores in the soil matrix, while their formation mechanism had not clearly explored yet. By-product lignin stabilized soils suffered from performance deterioration as exposed to moisture intrusion and wetting-drying cycle. The formed bonding among soil particles was essential in affecting the mechanical responses and durability of stabilized soils. The desirable construction procedure and protective measure were encouraged to be established for safety applications of the stabilized soils. Additional researches were recommended to by-product lignin optimization/modification, dynamic behaviors of stabilized soils, and application in some special soils. The outcomes of this review are invaluable in facilitating application of biomass by-product lignin for stabilizing problematic soils in engineering constructions.

5.
Analyst ; 145(9): 3353-3358, 2020 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-32226994

RESUMO

MicroRNAs (miRNAs) are desirable targets for the diagnosis, prognosis and treatment of diverse human diseases. In this study, we developed a universal and enzyme-free signal amplification approach for the quick, sensitive and specific electrochemical detection of diverse miRNAs from tumor cells using a catalyzed hairpin assembly (CHA) and the DNA three-way junction (DNA TWJ). The target miRNA, which was formed as an initiator through specific recognition, periodically triggered the assembly of two hairpins into a duplex via CHA. Subsequently, these duplexes induced the catalytic assembly of the DNA three-way junction and the release of the methylene blue-labeled DNA strand S (S-MB). Thereafter, the S-MB was captured using the electrode through hybridization with the capture probe to generate a notable current response. The proposed biosensor could be switched in response to different miRNA targets by changing the specific sequence of H0. These findings indicate its ability to distinguish single base mismatch miRNAs and analyze miRNAs extracted from cells. Therefore, the universal and enzyme-free signal amplified electrochemical method described herein can be potentially useful in diverse miRNA-related clinical analysis and disease diagnosis.

6.
Artigo em Inglês | MEDLINE | ID: mdl-32183048

RESUMO

A typical sodium activated calcium bentonite (SACaB) was treated with carboxymethyl cellulose (CMC) polymer, called CMC-treated SACaB (CMC-SACaB), and it was investigated for its hydraulic conductivity and enhanced chemical compatibility. Index property and hydraulic conductivity tests were conducted on CMC-SACaB and SACaB with deionized water (DIW), heavy metals-laden water, and actual landfill leachate. Lead-zinc mixed (Pb-Zn) solution and hexavalent chromium (Cr(VI)) solution were selected as target heavy metals-laden water, and calcium (Ca) solution was tested for comparison purposes. The hydraulic conductivity (kMFL) was determined via the modified fluid loss (MFL) test. Liquid limit and swell index in DIW, heavy metal-laden water, and Ca solution increased with increasing CMC content. CMC treatment effectively decreased the kMFL of SACaB when exposed to Pb-Zn solutions with a metal concentration of 1 to 20 mmol/L and landfill leachate. An insignificant change in kMFL of CMC-SACaB occurred with exposure to Pb-Zn solutions with metal concentrations of 1 to 10 mmol/L, Cr(VI) and Ca solutions with metal concentration of 1 to 20 mmol/L, and landfill leachate. A slight increase in kMFL of CMC-SACaB was observed when Pb-Zn concentration increased to 20 mmol/L, and such an increment was more noticeable when the CMC content was lower than 10%. In the DIW, the measured kMFL values of CMC-SACaB and SACaB with a given range of void ratio were consistent with those obtained from the flexible-wall permeameter test.

7.
J Food Sci ; 85(4): 956-963, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32159235

RESUMO

Large deformation characteristics in biaxial tensile and stress relaxation, and small deformation properties in frequency sweeping and microstructure of dough produced by three kinds of mixers were assessed in this study. Differences in noodle quality were also compared. Results indicated that dough made by vacuum or spiral mixer had good resistance to external force and good resilience. But the dough and noodles made by vacuum mixer showed the best tensile and cooking properties respectively, and had overall the best performance. Confocal microscopy revealed that the protein matrix of the three doughs formed along the direction of shear flow, whereas the starch granules of dough made by the vacuum mixer were tightly wrapped in gluten, resulting in a compact and sequential gluten network. Other samples showed signs of minor structural damage (pin mixer) or the presence of holes (spiral mixer). Assessment using Pearson correlation analysis identified a number of significant correlation coefficients between the dough rheological characteristics and noodle quality. Characterization of biaxial tensile and stress relaxation was advantageous for noodle assessment prediction. The index of flow and degree of deformation were negatively correlated with tensile properties. Frequency scanning is superior in predicting cooking and stretching characteristics of noodles. PRACTICAL APPLICATION: The research provides technical guidance for obtaining better dough in noodle processing.

8.
Sci Total Environ ; 716: 137095, 2020 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-32059325

RESUMO

Engineered Cementitious Composite (ECC) is proposed as a promising vertical cutoff wall material to contain acid mine drainage (AMD). The study presents comprehensive investigations of hydraulic conductivity of ECC permeated with AMD and self-healing of ECC subjected to wet-dry cycles. The effectiveness of incorporating reactive magnesia (MgO) into ECC for self-healing enhancement is also investigated. The chemical species formed in ECC and MgO-ECC specimens after exposure to AMD are investigated via SEM, FTIR, XRD and TGA analyses. The results show hydraulic conductivity of un-cracked and cracked ECC and MgO-ECC specimens pre-strained up to 1.32% is below commonly accepted limits of 10-8 m/s when permeated with AMD. The self-healing capacity of ECC specimens subjected to wet-dry cycles using both tap water and AMD as immersing liquids is improved by MgO addition. MgO addition is also beneficial for reducing hydraulic conductivity of un-cracked and cracked ECC specimens permeated with AMD. MgO addition results formation of new self-healing products including hydromagnesite and brucite when exposed to tap water, and hydrotalcite-like phase (Ht) when exposed to AMD.

10.
Artigo em Inglês | MEDLINE | ID: mdl-31935850

RESUMO

This study investigated the feasibility of using sodium hexametaphosphate (SHMP)- amended calcium (Ca) bentonite in backfills for slurry trench cutoff walls for the containment of lead (Pb) contamination in groundwater. Backfills composed of 80 wt% sand and 20 wt% either Ca-bentonite or SHMP-amended Ca-bentonite were tested for hydraulic conductivity and sorption properties by conducting laboratory flexible-wall hydraulic conductivity tests and batch isothermal sorption experiments, respectively. The results showed that the SHMP amendment causes a one order of magnitude decrease in hydraulic conductivity of the backfill using tap water (1.9 to 3.0 × 10-10 m/s). Testing using 1000 mg/L Pb solution resulted insignificant variation in hydraulic conductivity of the amended backfill. Moreover, SHMP-amendment induced favorable conditions for increased sorption capacity of the backfill, with 1.5 times higher retardation factor relative to the unamended backfill. The Pb transport modeling through an hypothetical 1-m-thick slurry wall composed of amended backfill revealed 12 to 24 times of longer breakthrough time for Pb migration as compared to results obtained for the same thickness slurry wall with unamended backfill, which is attributed to decrease in seepage velocity combined with increase in retardation factor of the backfill with SHMP amendment. Overall, SHMP is shown to be a promising Ca-bentontie modifier for use in backfill for slurry trench cutoff wall for effective containment of Pb-contaminated groundwater.


Assuntos
Cálcio/química , Água Subterrânea/química , Chumbo/química , Fosfatos/química , Areia , Poluentes Químicos da Água/química , Bentonita , Água/química , Poluição da Água/prevenção & controle
11.
J Sci Food Agric ; 99(14): 6500-6508, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31321768

RESUMO

BACKGROUND: Emulsification is important for food quality and processing functionality. Most emulsification occurs under high-fat conditions that eventually cause health concerns. Protein emulsifiers also have drawbacks such as lower dispersity. This study considered the effects of different high-speed shear homogenization (HSH) speeds on the emulsifying and structural properties of myofibrillar proteins (MPs) under low-fat conditions. RESULTS: High-speed shear homogenization significantly increased the emulsifying activity and emulsifying stability of MPs at lower speeds (8000 to 14 500 rpm). The primary structure of MP was not altered significantly by HSH, whereas its secondary, tertiary, and quaternary structures were changed. Particle size decreased first and then increased significantly, and reached a minimum when the HSH speed was 14 500 rpm. The absolute zeta potential values increased significantly and the dendritic fibrous structure of sample was destroyed when the speed exceeded 14 500 rpm. High-speed shear homogenization (14 500 rpm) decreased the particle size and unfolded the protein, which improved the emulsifying properties of MPs. Excessive HSH speeds (20 500 rpm or higher) caused an aggregation of MP molecules, which was not conducive to improving their emulsifying properties. CONCLUSION: Optimal HSH speed was achieved at 14 500 rpm to modify MPs' emulsifying and structural properties under low-fatconditions. © 2019 Society of Chemical Industry.


Assuntos
Gorduras/análise , Manipulação de Alimentos/métodos , Proteínas Musculares/química , Animais , Galinhas , Emulsões/química , Emulsões/isolamento & purificação , Manipulação de Alimentos/instrumentação , Carne/análise , Proteínas Musculares/isolamento & purificação , Tamanho da Partícula , Pressão , Dobramento de Proteína
12.
Sci Total Environ ; 680: 79-90, 2019 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-31102831

RESUMO

Diatoms and other phytoplankton groups are exposed to abrupt changes in pCO2, in waters in upwelling areas, near CO2 seeps, or during their blooms; or to more gradual pCO2 rise through anthropogenic CO2 emissions. Gradual CO2 rises have, however, rarely been included in ocean acidification (OA) studies. We therefore compared how small (Thalassiosira pseudonana) and larger (Thalassiosira weissflogii) diatom cell isolates respond to gradual pCO2 rises from 180 to 1000 µatm in steps of ~40 µatm with 5-10 generations at each step, and whether their responses to gradual pCO2 rise differ when compared to an abrupt pCO2 rise imposed from ambient 400 directly to 1000 µatm. Cell volume increased in T. pseudonana but decreased in T. weissflogii with an increase from low to moderate CO2 levels, and then remained steady under yet higher CO2 levels. Growth rates were stimulated, but Chl a, particulate organic carbon (POC) and cellular biogenic silica (BSi) decreased from low to moderate CO2 levels, and then remained steady with further CO2 rise in both species. Decreased saturation light intensity (Ik) and light use efficiency (α) with CO2 rise in T. pseudonana indicate that the smaller diatom becomes more susceptible to photoinhibition. Decreased BSi/POC (Si/C) in T. weissflogii indicates the biogeochemical cycles of both silicon and carbon may be more affected by elevated pCO2 in the larger diatom. The different CO2 modulation methods resulted in different responses of some key physiological parameters. Increasing pCO2 from 180 to 400 µatm decreased cellular POC and BSi contents, implying that ocean acidification to date has already altered diatom contributions to carbon and silicon biogeochemical processes.


Assuntos
Dióxido de Carbono/análise , Diatomáceas/fisiologia , Monitoramento Ambiental , Concentração de Íons de Hidrogênio , Fitoplâncton , Água do Mar/química
13.
Int J Biol Macromol ; 134: 230-236, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31047924

RESUMO

Active films based on chitosan (CS) and gum arabic (GA) incorporated with cinnamon essential oil (CEO) have been developed, and the effect of CS/GA ratios on the film structure, physical and antioxidant properties were investigated. Possible interactions among CS, GA and CEO was evaluated using rheological studies, Fourier transform infrared Spectroscopy (FT-IR) and X-ray diffraction (XRD). These results showed that there existed electrostatic interactions between CS and GA, which led to the formation of entangled structure. The addition of GA enhanced the water barrier properties of films, while it became less resistant to breakage and stretching. Confocal laser scanning microscopy (CLSM) results confirmed the higher CEO retention in films. Greatly enhanced antioxidant effectiveness were observed when the ratio of CS/GA changed from 1:0 to 1:2. However, when the ratio reached 1:4, the antioxidant capacity quickly decreased, which could be attributed to the negative influence of maximal interactions between CS and GA, leading to the loss of CEOs.


Assuntos
Antioxidantes/química , Quitosana/química , Cinnamomum zeylanicum/química , Goma Arábica/química , Óleos Voláteis/química , Compostos de Bifenilo/química , Fenômenos Químicos , Peróxido de Hidrogênio/química , Fenômenos Mecânicos , Picratos/química , Reologia
14.
Carbohydr Polym ; 217: 116-125, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31079667

RESUMO

Chitosan-gum arabic-based polyelectrolyte complexed films with cinnamon essential oil (CEO) and clove essential oil (CLO) were developed. The effect of EO concentrations, types and their combinations on the physical, thermal and antimicrobial properties of films were investigated. The results showed that the incorporation of EOs decreased the ζ-potential and viscosity, but increased the particle size of film-forming dispersions. Films incorporated with CEO and combined EOs exhibited better water barrier properties compared to those with CLO and single EO. Films containing CEO showed lower EO loss and higher thermal stability compared to those containing CLO, and the reason was attributed to the stronger interactions between chitosan, gum arabic and CEO. The combination of EOs resulted in higher retention and delayed release rate in food stimulant, resulting in stronger antimicrobial activities. The performance of films with the CEO and the combined EOs brought new formulation ideas in antimicrobial films.


Assuntos
Antibacterianos/farmacologia , Quitosana/farmacologia , Goma Arábica/farmacologia , Óleos Voláteis/farmacologia , Antibacterianos/química , Quitosana/química , Cinnamomum zeylanicum/química , Escherichia coli/efeitos dos fármacos , Goma Arábica/química , Membranas Artificiais , Testes de Sensibilidade Microbiana , Óleos Voláteis/química , Tamanho da Partícula , Staphylococcus/efeitos dos fármacos , Syzygium/química , Temperatura , Resistência à Tração , Viscosidade
15.
Org Lett ; 21(12): 4717-4720, 2019 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-31145625

RESUMO

A catalytic asymmetric ring-opening reaction between α-isocyanoacetates and meso-aziridines has been realized by developing an in-situ-generated magnesium catalytic method. Chiral oxazoline-OH ligands were employed in the magnesium catalyst and diphenylphosphinamide was improved as a powerful achiral additive in this reaction. The ring-opening products of the desired reaction were obtained in good chemical yields and enantioselectivities. Moreover, these enantio-enriched adducts can be smoothly transformed into tetrahydropyrimidines mediated by a silver salt under mild conditions.

16.
Chem Commun (Camb) ; 55(37): 5387-5390, 2019 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-30997454

RESUMO

DNA-triggered reversible isolation and recovery of circulating tumor cells (CTCs) is presented based on a multivalent dual-specific aptamer-tethered rolling circle amplification (MA-RCA) network. The multivalent binding sites endow the MA-RCA network with a strong binding ability towards CTCs, and the repeated cell capture/release processes are also actualized in a noninvasive manner.


Assuntos
Aptâmeros de Nucleotídeos/química , DNA/química , Células Neoplásicas Circulantes/patologia , Linhagem Celular Tumoral , DNA/metabolismo , DNA de Cadeia Simples/química , DNA de Cadeia Simples/metabolismo , Corantes Fluorescentes/química , Humanos , Microscopia de Fluorescência , Células Neoplásicas Circulantes/química , Células Neoplásicas Circulantes/metabolismo , Técnicas de Amplificação de Ácido Nucleico
17.
J Hum Genet ; 64(7): 681-687, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31019231

RESUMO

Weill-Marchesani syndrome (WMS) is a rare connective tissue disorder characterized by short stature, brachydactyly, joint stiffness, eye anomalies, including microspherophakia, ectopia of the lenses, severe myopia, glaucoma and occasionally heart defects. Given these complex clinical manifestations and genetic heterogeneity, WMS patients presented misdiagnosed as high myopia or angle closure glaucoma. Here, we report ADAMTS17 mutations, a member of the extracellular matrix protease family, from a Chinese family. Patients have features that fall within the WMS spectrum. The exome (protein-coding regions of the genome) makes up ~1 % of the genome, it contains about 85% of known disease-related variants. Whole exome sequencing (WES) has been performed to identify the disease-associated genes, including one patient, his healthy sister, and his asymptomatic wife. Genome-wide homozygosity map was used to identify the disease caused locus. SNVs and INDELs were further predicted with MutationTaster, LRT, SIFT and SiPhy and compared to dbSNP150 and 1000 Genomes project. Filtered mutation was confirmed with Sanger sequencing in whole family members. The Genome-wide homozygosity map based on WES identified a total of 20 locus which were possible pathogenic. Further, a novel nonsense mutation c.1051A >T result in p.(lys351Ter) in ADAMTS17 had been identified in a candidate loci. The Sanger sequencing data has verified two consanguineous WMS patients in the family pedigree and revealed autosomal recessive (AR) inheritance pattern. The nonsense mutation in ADAMTS17 was analyzed in silico to explore its effects on protein function. We predicted the mutation produced non-function protein sequence. A novel nonsense mutation c.1051 A > T in ADAMTS17 had been identified caused autosomal recessive WMS in the Chinese family.


Assuntos
Proteínas ADAMTS/genética , Códon sem Sentido , Síndrome de Weill-Marchesani/genética , Adulto , Criança , China , Mapeamento Cromossômico , Nanismo/genética , Anormalidades do Olho/genética , Feminino , Homozigoto , Humanos , Masculino , Linhagem , Síndrome de Weill-Marchesani/diagnóstico , Sequenciamento Completo do Exoma , Adulto Jovem
18.
Small ; 15(16): e1900011, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30913378

RESUMO

Organic framework polymers have attracted much interest due to the enormous potential design space offered by the atomically precise spatial assembly of organic molecular building blocks. The morphology control of organic frameworks is a complex issue that hinders the development of organic frameworks for practical applications. Biomimetic self-assembly is a promising approach for designing and fabricating multiple-functional nanoarchitectures. A bioinspired hollow flower-like organic framework nanosphere heterostructure comprised of carbon nitride and polydopamine (g-C3N4@PDA) is successfully synthesized via a mild and green method. This heterostructure can effectively avoid the agglomeration of nanosheets to better access the hollow nanospheres with high open-up specific surface area. The electron delocalization of g-C3N4 and PDA under visible light can largely promote photoelectron transfer and enhance the photocatalytic activity of the g-C3N4@PDA. Furthermore, the g-C3N4@PDA can effectively enhance the generation of reactive oxygen species under irradiation, which can lead to cell apoptosis and enhance the performance for cancer therapy. Therefore, the as-prepared g-C3N4@PDA provides a paradigm of highly efficient photocatalyst that can be used as nanomedicine toward cancer therapy. This study could open up a new avenue for exploiting more other potential hollow nanosphere organic frameworks.

19.
Bioorg Chem ; 87: 447-456, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-30925429

RESUMO

In our continuous search for new nitric oxide (NO) inhibitory compounds as potential anti-inflammatory agents or lead compounds for inflammatory diseases, the chemical constituents of Euonymus verrucosus var. pauciflorus were investigated, leading to the isolation of eleven terpenoids including six new diterpenoids, designated as euonymupenes A-F. The structures were elucidated on the basis of NMR and ECD data analysis. Euonymupenes A, C, and F feature rare labdane-type norditerpenoid skeletons. The NO inhibitory effects were evaluated and all of the isolates were found to inhibit lipopolysaccharide (LPS)-induced NO production in murine microglial BV-2 cells. Western blotting analysis indicated that the most active compound (5) can regulate iNOS (inducible nitric oxide synthase) expression. The further molecular docking studies exhibited the affinities of bioactive compounds with iNOS.

20.
J Cell Mol Med ; 23(5): 3271-3279, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30825262

RESUMO

The triple-negative breast cancer is the most malignant type of breast cancer. Its pathogenesis and prognosis remain poor despite the significant advances in breast cancer diagnosis and therapy. Meanwhile, long noncoding RNAs (LncRNAs) play a pivotal role in the progression of malignant tumors. In this study, we found that LncRNA-ZEB2-AS1 was dramatically up-regulated in our breast cancer specimens and cells (MDA231), especially in metastatic tumor specimens and highly invasive cells, and high lncRNA-ZEB2-AS1 expression is associated with clinicopathologic features and short survival of breast cancer patients. LncRNA-ZEB2-AS1 promotes the proliferation and metastasis of MDA231 cells in SCID mice. Thus, it is regarded as an oncogene in triple-negative breast cancer. It is mainly endo-nuclear and situated near ZEB2, positively regulating ZEB2 expression and activating the epithelial mesenchymal transition via the PI3K/Akt/GSK3ß/Zeb2 signaling pathway. Meanwhile, EGF-induced F-actin polymerization in MDA231 cells can be suppressed by reducing lncRNA-ZEB2-AS1 expression. The migration and invasion of triple-negative breast cancer can be altered through cytoskeleton rearrangement. In summary, we demonstrated that lncRNA-ZEB2-AS1 is an important factor affecting the development of triple-negative breast cancer and thus a potential oncogene target.

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