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1.
J Pharm Biomed Anal ; 177: 112890, 2020 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-31563756

RESUMO

Various immunoassay methods have been developed and used for the therapeutic drug monitoring (TDM) of cyclosporine (CsA). However, there is no report on the application of a time-resolved fluoroimmunoassay (TRFIA) in routine CsA TDM. The aim of this study was to evaluate the feasibility and validate the performance of a newly developed TRFIA method for CsA analysis in human whole blood. The TRFIA method was then compared with the method of chemiluminescent microparticle immunoassay (CMIA). The calibration range of the CsA-TRFIA method was 0-1000 ng/mL. The linear range and correlation coefficients were 30-1000 ng/mL and more than 0.990, respectively. The accuracy, precision, and inter-batch range were 90.0%-110.0%, less than 10%, and no more than 15%, respectively. The lowest limit of detection was less than 10 ng/mL. The linear regression equation was YCMIA = 0.961XTRFIA + 3.357, which showed that the measurements of CMIA and TRFIA were strongly correlated (r = 0.980). The results demonstrate that TRFIA is a precise and reproducible method for detecting the CsA concentration and can be used for routinely CsA TDM.

2.
Food Chem ; 307: 125568, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31630021

RESUMO

Sacha inchi oil is derived from the seeds of Plukenetia volubilis L. and has great nutritional value due to its high contents of active polyunsaturated triacylglycerols (PUTAGs). In this study, we developed a methodology combined Paternò-Büchi reaction nanoelectrospray ionization mass spectrometry (PB-nanoESI-MS) and nuclear magnetic resonance (NMR) to identify CC locations and isomers of PUTAGs in sacha inchi oil. Benzophenone was used as the PB reagent, and the optimized solvent composition (methanol:chloroform = 9:1) allowed for PUTAGs and their PB products to be detected with higher intensities. In addition, we made efforts to interpret the MS2 spectra for identification lipid species. A series of C57-PUTAGs and C59-PUTAGs were detected and identified via high-resolution PB-nanoESI-MS, and the predominant PUTAGs were TAG 18:1(Δ9)_18:3(Δ9,12,15)_18:3(Δ9,12,15) and TAG 18:2(Δ9,12)_18:2(Δ9,12)_18:3 (Δ9,12,15), which demonstrated that the PB-nanoESI-MS approach in this study provides help in promoting the development of structural determination of triacylglycerols in food chemistry.

3.
Nat Nanotechnol ; 14(11): 1048-1055, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31686005

RESUMO

Many living organisms track light sources and halt their movement when alignment is achieved. This phenomenon, known as phototropism, occurs, for example, when plants self-orient to face the sun throughout the day. Although many artificial smart materials exhibit non-directional, nastic behaviour in response to an external stimulus, no synthetic material can intrinsically detect and accurately track the direction of the stimulus, that is, exhibit tropistic behaviour. Here we report an artificial phototropic system based on nanostructured stimuli-responsive polymers that can aim and align to the incident light direction in the three-dimensions over a broad temperature range. Such adaptive reconfiguration is realized through a built-in feedback loop rooted in the photothermal and mechanical properties of the material. This system is termed a sunflower-like biomimetic omnidirectional tracker (SunBOT). We show that an array of SunBOTs can, in principle, be used in solar vapour generation devices, as it achieves up to a 400% solar energy-harvesting enhancement over non-tropistic materials at oblique illumination angles. The principle behind our SunBOTs is universal and can be extended to many responsive materials and a broad range of stimuli.

4.
Biomed Res Int ; 2019: 5907361, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31737670

RESUMO

Objectives: Obstructive sleep apnea (OSA) is a common disorder influenced by genetic and environmental factors. Mutations of AT-hook DNA-binding motif containing 1 (AHDC1) gene have been implicated which could cause rare syndromes presenting OSA. This study aims to investigate some rare mutations of AHDC1 in Chinese Han individuals with OSA. Patients and Methods: Three hundred and seventy-five patients with OSA and one hundred and nine control individuals underwent polysomnography. A targeted sequencing experiment was taken in 100 patients with moderate-to-severe OSA, and genotyping was taken in 157 moderate-to-severe OSA and 100 control individuals. The effect of mutations was validated by the luciferase reporter assay. Results: One rare missense mutation (AHDC1: p.G1484D) and two mutations (c.-88C>T; c.-781C>G) in 5'-untranslated region (UTR) of AHDC1 were identified. The rare mutation (c.-781C>G) in 5'-UTR that was identified in several patients presenting more severe clinical manifestations affects expression of AHDC1. Conclusions. Our results revealed three rare mutations of AHDC1 in patients with OSA in Chinese Hanindividuals.

5.
Analyst ; 144(18): 5637-5645, 2019 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-31433404

RESUMO

The simultaneous analysis of perfluoroalkyl substances (PFASs) and lipids in biological tissues is of importance, especially for in situ and microscale analysis, because it provides significant information to understand the relevance of content, composition, and distribution of lipids to the bioaccumulation of PFASs as well as lipid metabolism affected by the biotoxicity of PFASs. In this study, we report the development of a novel ambient mass spectrometry method for the rapid, in situ, and microscale analysis of PFASs and lipids simultaneously in biological tissues for the investigation of their biological correlation. A microscale solid-phase microextraction (SPME) probe with a probe-end diameter of several-µm was employed for in situ and microscale sampling of biological tissues after PFAS exposure. The SPME probe showed a desirable capacity for the enrichment of PFASs and lipid species simultaneously. After sampling and extraction, the loaded SPME probe was directly applied for nanoESI-MS analysis under ambient and open-air conditions. A high-resolution Fourier transform ion cyclotron resonance (FTICR) mass spectrometer operated in the field-induced mode was introduced to record mass spectra using fast polarity switching between positive and negative ion detection. Most of the lipid species were recorded in the positive ion mass spectrum, and PFASs were recorded in the negative ion mass spectrum. By using the developed method, the in situ analysis of PFASs and lipids in the muscle, brain, heart, kidney, liver, and intestine of zebrafish was realized. In addition, simultaneously imaging PFASs and lipids in individual Daphnia magna was successfully achieved for the investigation of their biological correlation.

6.
ACS Synth Biol ; 8(9): 2113-2120, 2019 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-31386360

RESUMO

Microorganisms often use specific autoinducers other than common metabolites for quorum sensing (QS). Herein, we demonstrated that Escherichia coli produced sulfide (H2S, HS-, and S2-) with the concentrations proportionally correlated to its cell density. We then designed synthetic gene circuits that used H2S as an autoinducer for quorum sensing. A sulfide/quinone oxidoreductase converted diffusible H2S to indiffusible hydrogen polysulfide (HSnH, n ≥ 2), and a gene regulator CstR sensed the latter to turn on the gene expression. We constructed three element libraries, with which 24 different circuits could be assembled for adjustable sensitivity to cell density. The H2S-mediated gene circuits endowed E. coli cells within the same batch or microcolony with highly synchronous behaviors. Using them we successfully constructed cell factories capable of an autonomous switch from growth phase to production phase. Thus, these circuits provide a new tool-kit for metabolic engineering and synthetic biology.

7.
Lung ; 197(5): 577-584, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31372721

RESUMO

PURPOSE: Obstructive sleep apnea (OSA) is a common sleep disorder that is influenced by various environmental and genetic factors. The potential associations of leptin and leptin receptor (LEPR) polymorphisms with OSA have been studied in different populations; however, the results remain inconclusive. The aim of this study was to examine the association between LEPR gene polymorphisms and OSA risk. METHODS: A total of 322 samples were used, including 226 OSA subjects and 96 controls. Targeted sequencing of the entire LEPR gene was performed in all subjects. Polysomnography was used to diagnose obstructive sleep apnea. The associations between variants and OSA were determined by multivariate regression analyses. RESULTS: Four single-nucleotide polymorphisms of LEPR were identified in all subjects. The genotype frequency of locus rs3790435 was significantly different between the OSA and control groups. Specifically, the variant genotype rs3790435 CC in LEPR was associated with a lower risk of OSA (OR 0.462, 95% CI 0.250-0.854, p = 0.014) in a recessive model after controlling for potential confounders. After BMI stratification, obese patients with this variant genotype were found to have a lower risk of developing OSA. Moreover, subjects with the rs3790435 CC genotype were found to have a statistically lower apnea-hypopnea index (AHI) and higher nadir oxygen saturation than the TT/CC genotypes without differences in plasma leptin levels. CONCLUSIONS: Our study identified a novel variant of LEPR in patients with OSA, and specifically found an association between rs3790435 polymorphisms and OSA risk in Chinese Han subjects.

8.
Talanta ; 204: 238-247, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31357288

RESUMO

In this article, an ambient mass spectrometric method with molecularly imprinted polymer (MIP)-coated wooden tips was developed for sensitive analysis of trace macrolide antibiotics in complex food samples. A novel solid-phase microextraction (SPME) probe was prepared, via the modification of a layer MIP coating (with roxithromycin as template molecule) on the surface of wooden tips. The obtained MIP-coated wooden-tip SPME probe can be applied directly to enrich trace macrolide antibiotics from complex food samples, with enrichment factors of 244-1604, 72-370, and 12-82 folds for analysis of five investigated macrolide antibiotics in drinking water, honey, and milk samples, respectively. After extraction, a high voltage and some spray solvent were applied on the loaded SPME probe to desorb and ionize analytes enriched on the probe surface for electrospray ionization mass spectrometric (ESI-MS) analysis under ambient and open-air conditions. The method showed good linearity, with correlation coefficient values (r2) no less than 0.9904, and the calibration function was verified via Mandel's fitting test (p > 0.063). The limits of detection were in the range of 0.003-0.05, 1.1-5.1, and 1.9-15.8 ng/g for analysis of drinking water, honey, and milk samples, respectively. Recoveries of the five targeted macrolide antibiotics in honey and milk samples ranged from 73.4% to 98.1%, with the standard deviations no higher than 8.6%. As a result, MIP-coated wooden-tip ESI-MS method could be feasibly used as a sensitive method for determination of trace macrolide antibiotics in complex food samples.

9.
Mol Genet Genomic Med ; 7(8): e846, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31293107

RESUMO

BACKGROUND: Multiple genetic and environmental factors contribute to the individual-level heterogeneity in stroke. This study aimed to assess how the genetic interactions confer risk of stroke. METHODS: In a Chinese case-control study including 1,405 strokes and 1,263 controls who were followed up (range, 0.1-6.0 years), eight genes, including apolipoprotein(a) (APOA1), methylenetetrahydrofolate reductase (MTHFR), vitamin K epoxide reductase complex subunit 1 (VKORC1), arachidonate 5-lipoxygenase-activating protein (ALOX5AP), NOTCH3, chromosome 9p21.3(Chr.9p21.3), vascular endothelial growth factor (VEGFA), and kinase insert domain-containing receptor (KDR), were analyzed for interactions by the generalized multifactor dimensionality reduction method and validated by the multivariate logistic regression models. The genetic associations with carotid artery intima-media thickness (IMT) were examined. RESULTS: The interaction of VKORC1 and Chr.9p21.3 was identified for stroke and its worse prognosis, and subjects having the VKORC1 rs2359612C and Chr.9p21.3 rs10757274G alleles had higher risks for stroke (OR = 1.83, 95% CI = 1.32-2.52) as well as for stroke recurrence (HR = 1.84, 95% CI = 1.24-2.73), cardiovascular events (HR = 1.65, 95% CI = 1.15-2.38), and cardiovascular mortality (HR = 2.16, 95% CI = 1.24-3.79). Supporting, they were associated with higher IMT. Hypertension or physical inactivity increased the risk effect. The interaction of VEGFA rs833061C and KDR rs2305948T was identified for hemorrhagic stroke. CONCLUSIONS: Our findings identified two novel genetic interactions of VKORC1 and Chr.9p21.3 and of VEGFA and KDR for risk of stroke and subtypes as well as future stroke prognosis.

10.
Arch Environ Occup Health ; : 1-14, 2019 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-31210102

RESUMO

To investigate the contamination levels of respirable dust released in the work environment and the induced workers' health risk at a coal-fired power plant, we collected 405 dust samples from different dusty workstations by personal sampling during the coal-fired power generation process. Then, an inhalation risk assessment model from the USEPA was combined with the Monte Carlo simulation method to quantitatively evaluate the health risk caused by dust inhalation. Of 10 workstations researched, the dust concentration in the most workstations exceeded the prescribed occupational exposure limit. Workers engaged in ash removal suffered the highest health risk at 4.08 × 10-6 ± 2.85 × 10-6 (95% CI), closely followed by those involved in other job categories. The results can contribute to the formulation of targeted dust prevention measures and implementation of risk management for the coal-fired power sector.

11.
Sleep Breath ; 2019 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-31044373

RESUMO

PURPOSE: Obstructive sleep apnea (OSA) is a common disorder characterized by recurrent episodes of partial or complete upper airway obstruction. OSA susceptibility is associated with multiple genetic, environmental, and developmental factors. The PPARG rs1801282 (G/C) polymorphism has been associated with OSA in obese Indian subjects, whereas no such association has been reported in Chinese Han subjects. Potential associations between other PPARG variants and OSA have not been investigated in Chinese Han populations. The aim of this study was to identify genetic variants of PPARG in unrelated Chinese Han patients with OSA and to investigate potential associations between these variants and OSA. METHODS: We performed a cross-sectional study of 233 individuals with OSA and 93 control individuals. A portable diagnostic device was used to diagnose OSA. Targeted sequencing was conducted to identify PPARG variants. Associations between PPARG variants and OSA were analyzed using multivariate regression. RESULTS: Three PPARG single-nucleotide polymorphisms were identified and the genotype frequencies of the rs1801282 polymorphism differed significantly. Subjects with the PPARG rs1801282 CG genotype had decreased risk of having OSA compared with subjects with the CC genotype after adjusting for confounding effects. CONCLUSIONS: We identified a variant of PPARG associated with the occurrence of OSA in Chinese Han populations.

12.
Artigo em Inglês | MEDLINE | ID: mdl-31109043

RESUMO

China's coal mine production situation is grim and various types of accidents occur frequently, and gas explosion accidents are the highest incidence of coal mine accidents. The authors selected 200 gas explosion accidents of coal mine enterprises in recent years, and extracted a large number of workers' unsafe behaviors. Meanwhile, four working types related to gas explosion accidents were obtained, namely ventilation, gas prevention and fire extinguishing, blasting, and electrician. This article listed some influencing factors of unsafe behaviors and corrected the probability of unsafe behaviors. In addition, a probabilistic risk assessment model was established, and the Monte Carlo method was used to analyze the risks caused by unsafe behaviors of various working types. The results show that the risk of unsafe behaviors caused by the ventilation working type is the highest, followed by gas prevention and fire extinguishing, and finally blasting and electrician. This paper studies the influencing factors of miners' unsafe behaviors from the perspective of behavior, guarantees effectively the safety management of coal mine enterprises, and lays a foundation for studying unsafe behaviors related to coal mine gas explosions.


Assuntos
Acidentes de Trabalho , Minas de Carvão , Explosões , Mineradores , China , Humanos , Medição de Risco , Gestão da Segurança
13.
Medicine (Baltimore) ; 98(16): e15219, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31008949

RESUMO

Obstructive sleep apnoea (OSA) is a prevalent sleep disorder considered as an independent risk factor for cardiovascular consequences. It has a strong genetic background and is associated with hypoadiponectinaemia.Target sequencing of whole ADIPONQ gene was performed in 340 participants including 247 patients with OSA and 93 non-OSA participants. Polysomnography was used to diagnose OSA. The associations between variants and OSA were determined by multivariate regression analysis.Thirteen single nucleotide polymorphisms of ADIPOQ were identified in all subjects. Genotype frequencies at rs4686803 (P = .034), rs3774262 (P = .034), and rs2082940 (P = .045) were significantly different between OSA and non-OSA groups. Individuals carrying the CT/TT genotypes of rs4686803, GA/AA genotypes of rs3774262, and CT/TT genotypes of rs1063537 were associated with 2.295-, 2.295- and 2.155-fold increased risk of OSA respectively in dominant model, after adjusting for confounding effects. The subjects with the rs2082940 CC genotype were associated with decreased risk of OSA (OR: 0.455) in recessive model. Additionally, the apnoea-hypopnea index (AHI) was significantly increased in rs3774262 (GA/AA) (P = .001), rs4686803 (CT/TT) (P = .001), and rs1063537 (CT/TT) (P = .004) genotype individuals than those with rs3774262 (GG), rs4686803 (CC), and rs1063537 (CC) genotypes, respectively. The AHI was significantly decreased in individuals with ADIPOQ rs2082940 CC genotypes than in those with the CT and TT genotype (P = .007). Moreover, the stratified analysis found that the genotype of rs3774262 (GA/AA), rs4686803 (CT/TT), and rs1063537 (CT/TT) variants were associated with increased risk of OSA by 2.935-, 2.935- and 2.786-fold in overweight participants. The genotype of rs2082940 CC variants was associated with decreased risk of OSA (OR: 0.373) in overweight participants compared with rs2082940 CT/ TT genotypes.ADIPOQ variants rs3774262, rs4686803, rs1063537, and rs2082940 were associated with the prevalence of OSA in Chinese Han individuals.


Assuntos
Adiponectina/genética , Apneia Obstrutiva do Sono/genética , Adulto , Idoso , Grupo com Ancestrais do Continente Asiático , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Sobrepeso/complicações , Polimorfismo de Nucleotídeo Único , Análise de Sequência de DNA , Apneia Obstrutiva do Sono/sangue
14.
Anal Biochem ; 576: 13-19, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-30978317

RESUMO

Current sample processing (SP) methods for tacrolimus (FK506) immunoassays are mainly based on extraction of drug by organic solvent and divalent metal ions. Although these methods are effective for drug extraction and interference elimination, they suffer from drawbacks including inconvenience for operation, difficulties for automation and potential measurement bias. To overcome these limitations, this study describes a new SP reagent for blood cell lysis and protein denaturation. A TRFIA (time-resolved fluorescence immunoassay) was developed by using this SP reagent for whole blood FK506 quantification. Results show that blood samples could be turned into homogeneous solution after being treated by this SP reagent, and so could be directly applied to immunoassays without centrifugation. The analytical sensitivity of the FK506-TRFIA was 0.57 ng/mL, the within-run and between-run coefficient of variations (CVs) were both less than 10%. The FK506 values of 126 samples obtained by FK506-TRFIA correlated excellently with that obtained by ABBOTT FK506-CMIA (R2 = 0.982). Comparison studies also show that the FK506-TRFIA was highly resistant to endogenous interferences. These results suggest that the present SP method is a more promising chose for FK506 immunoassay, and in the meantime, its simplicity makes the whole-process immunoassay automation more feasible by obviating the necessary for centrifugation.

15.
Curr Pharm Des ; 25(2): 190-200, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30827231

RESUMO

BACKGROUND AND OBJECTIVE: Familial hypercholesterolemia (FH) is a severe genetic hyperlipidemia characterized by increased levels of low-density lipoprotein cholesterol (LDL-C), leading to premature atherosclerosis. Angiopoietin-like protein (ANGPTL3) is a hepatocyte-specific protein that can be used to lower LDL in FH. However, it was unknown whether ANGPTL3 variants are present in FH patients. This study was performed to identify ANGPTL3 variants in unrelated Chinese Han patients with FH. METHODS AND RESULTS: We screened 80 patients with FH (total cholesterol >7.8mmol/L, LDL-cholesterol >4.9mmol/L) and 77 controls using targeted next-generation sequencing (NGS) of six FH candidate genes (LDLR, ApoB100, PCSK9, ABCG5, ABCG8, and ANGPTL3). Candidate pathogenic variants identified by NGS were validated by Sanger sequencing. Mutant and wild-type plasmids containing the variant sequence were constructed and verified by Sanger sequencing. The gene expression profile was analyzed by an expression profile chip in transfected HepG2 cells using quantitative real-time (qRT)-PCR. We identified 41 variants in 28 FH patients, including two ANGPTL3 mutations: one exonic (c.A956G: p.K319R) and one in the untranslated region (c.*249G>A). Gene ontology analyses found that the cholesterol metabolic process and ANGPTL3 expression were significantly up-regulated in the ANGPTL3 K319R mutation group compared with the wild-type group. qRT-PCR findings were consistent with the expression profile analysis. CONCLUSION: Rare ANGPTL3 variants were identified in Chinese patients with FH, including ANGPTL3: p.(Lys319Arg) which affected the expression of ANGPTL3 and the cholesterol metabolic process as determined by bioinformatics analysis. CLINICAL TRIAL REGISTRATION: Chinese Clinical Trial Registration (ChiCTR-ROC-17011027) http://www.chictr.org.cn/listbycreater.aspx.

16.
Environ Geochem Health ; 41(5): 2179-2193, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30874937

RESUMO

With a growing awareness of environmental protection, the dust pollution caused by automobile foundry work has become a serious and urgent problem. This study aimed to explore contamination levels and health effects of automobile foundry dust. A total of 276 dust samples from six types of work in an automobile foundry factory were collected and analysed using the filter membrane method. Probabilistic risk assessment model was developed for evaluating the health risk of foundry dust on workers. The health risk and its influencing factors among workers were then assessed by applying the Monte Carlo method to identify the most significant parameters. Health damage assessment was conducted to translate health risk into disability-adjusted life year (DALY). The results revealed that the mean concentration of dust on six types of work ranged from 1.67 to 5.40 mg/m3. The highest health risks to be come from melting, cast shakeout and finishing, followed by pouring, sand preparation, moulding and core-making. The probability of the risk exceeding 10-6 was approximately 85%, 90%, 90%, 75%, 70% and 45%, respectively. The sensitivity analysis indicated that average time, exposure duration, inhalation rate and dust concentration (C) made great contribution to dust health risk. Workers exposed to cast shakeout and finishing had the largest DALY of 48.64a. These results can further help managers to fully understand the dust risks on various types of work in the automobile foundry factories and provide scientific basis for the management and decision-making related to health damage assessment.

17.
Immunity ; 50(4): 1043-1053.e5, 2019 04 16.
Artigo em Inglês | MEDLINE | ID: mdl-30902636

RESUMO

Human Vγ9Vδ2 T cells respond to microbial infections and malignancy by sensing diphosphate-containing metabolites called phosphoantigens, which bind to the intracellular domain of butyrophilin 3A1, triggering extracellular interactions with the Vγ9Vδ2 T cell receptor (TCR). Here, we examined the molecular basis of this "inside-out" triggering mechanism. Crystal structures of intracellular butyrophilin 3A proteins alone or in complex with the potent microbial phosphoantigen HMBPP or a synthetic analog revealed key features of phosphoantigens and butyrophilins required for γδ T cell activation. Analyses with chemical probes and molecular dynamic simulations demonstrated that dimerized intracellular proteins cooperate in sensing HMBPP to enhance the efficiency of γδ T cell activation. HMBPP binding to butyrophilin doubled the binding force between a γδ T cell and a target cell during "outside" signaling, as measured by single-cell force microscopy. Our findings provide insight into the "inside-out" triggering of Vγ9Vδ2 T cell activation by phosphoantigen-bound butyrophilin, facilitating immunotherapeutic drug design.


Assuntos
Antígenos CD/química , Butirofilinas/química , Ativação Linfocitária , Organofosfatos/metabolismo , Subpopulações de Linfócitos T/imunologia , Antígenos CD/metabolismo , Sítios de Ligação , Butirofilinas/metabolismo , Cristalografia por Raios X , Dimerização , Desenho de Drogas , Humanos , Ligações de Hidrogênio , Imunoterapia , Modelos Moleculares , Simulação de Dinâmica Molecular , Mutagênese Sítio-Dirigida , Conformação Proteica , Domínios Proteicos , Isoformas de Proteínas/química , Processamento de Proteína Pós-Traducional , Receptores de Antígenos de Linfócitos T gama-delta , Análise de Célula Única , Relação Estrutura-Atividade , Subpopulações de Linfócitos T/metabolismo
18.
Anal Chem ; 91(7): 4592-4599, 2019 Apr 02.
Artigo em Inglês | MEDLINE | ID: mdl-30832475

RESUMO

Lipids are important structural components of biological systems, and lipid C═C locations play important roles in their biophysical and biochemical properties. Rapid, in vivo, in situ, and microscale lipidomics investigation (including precise identification of lipid C═C locations and isomers) of biological specimen has great potential for clinical diagnosis, biological studies, and biomarker discovery. Here we report a novel lipidomics methodology by coupling Paternò-Büchi (PB) reaction with surface-coated probe nanoelectrospray ionization mass spectrometry (SCP-nanoESI-MS) for in vivo, in situ, and microscale analysis of lipid species and C═C location isomers in complex biological tissues. The proposed SCP-PB-nanoESI-MS method was performed by application of a biocompatible solid-phase microextraction (SPME) probe for in vivo, in situ, and microscale sampling and extraction of lipids from biological tissues, and then some spray solvent containing PB reagent was applied to desorb lipid species enriched on SPME probe within a nanospray tip. Subsequently, ultraviolet irradiation was employed to initiate PB reaction for unsaturated lipids within the nanospray tip. After that, a high voltage was applied on the SPME probe to induce nanoESI for MS analysis under ambient and open-air conditions, and collision-induced dissociation was performed to the PB reaction product ions for determination of lipid C═C locations and isomers. By using our proposed SCP-BP-nanoESI-MS method, microscale investigation of lipid compositions and C═C location isomers for lipid droplet of Perilla seed and human intestinal tissue were successfully achieved, and in vivo analysis of lipid species and C═C locations for zebrafish was accomplished.

19.
Artigo em Inglês | MEDLINE | ID: mdl-30717157

RESUMO

Miners' unsafe behavior is the main cause of roof accidents in coal mines, and behavior intervention plays a significant role in reducing the occurrence of miners' unsafe behavior. However, traditional behavior intervention methods lack pertinence. In order to improve the intervention effect and reduce the occurrence of coal mine roof accidents more effectively, this study proposed a targeted intervention method for unsafe behavior. The process of targeted intervention node locating was constructed, and based on the analysis of 331 coal mine roof accidents in China, three kinds of targeted intervention nodes were located. The effectiveness of targeted intervention nodes was evaluated by using structural equation model (SEM) through randomly distributing questionnaires to miners of Pingdingshan coal. The results show that, in preventing roof accidents of coal mines, the targeted intervention nodes have a significant positive impact on the intervention effect. The method can also be applied to the safety management of other industries by adjusting the node location and evaluation process.


Assuntos
Prevenção de Acidentes/métodos , Minas de Carvão , Comportamentos de Risco à Saúde/fisiologia , Mineradores/psicologia , Saúde do Trabalhador , Acidentes de Trabalho/prevenção & controle , Acidentes de Trabalho/estatística & dados numéricos , China , Humanos , Modelos Estatísticos , Gestão da Segurança/métodos
20.
Exp Gerontol ; 119: 212-220, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30776409

RESUMO

The role of angiotensin II (Ang II) in hypertension has been clarified, but recent studies show that aging-associated arterial changes and those with hypertension as well as atherosclerosis may have some common pathogenesis. This study aimed to clarify the effects of Ang II on endothelial senescence by establishing a replicative senescence model of human umbilical vein endothelial cells (HUVECs) in vitro. The population-doubling level (PDL) was calculated, PDL5 and PDL25 respectively referred to cells cultured for 2 days and 30 days. Compared with Ang II-treated young PDL5 cells, chronic stimulation of Ang II significantly promoted the senescence-associated ß-galactosidase activity and expression of senescence-related genes p16 and p21, slowed down cell growth rate, and decreased expression of longevity-related genes sirtuin1 as well as telomerase activity in senescent PDL25 cells (all P < 0.05). Moreover, expression of pro-inflammatory cytokines and adhesion molecules were up-regulated in Ang II-treated PDL25 cells (all P < 0.05). Ang II-induced senescent progression and inflammation were attenuated by angiotensin receptor blocker valsartan. In young PDL5 cells, Ang II promoted the endothelial viability including cell proliferation, migration, angiogenesis and cell adhesion to monocytes; however, chronic stimulation of Ang II suppressed the cell viability, promoted cell adhesion and apoptosis in senescent PDL25 cells, which could be ameliorated by short-term valsartan, but long-term valsartan had no effects. In addition, Ang II-induced senescent features could be partly recovered if Ang II was stopped at PDL20. These findings suggested that chronic stimulation of Ang II can accelerate the endothelial senescence process which is implicated in aging-related atherosclerosis.

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