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1.
Spectrochim Acta A Mol Biomol Spectrosc ; 266: 120432, 2022 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-34607092

RESUMO

Diabetes has become one of the three chronic non-communicable diseases threatening human health in the world, and the detection of glucose concentration is of great importance for the prevention and treatment of diabetes. The noninvasive detection of glucose in tears has attracted interest over the past several decades, however, time-consuming, expensive equipment, and specialist technicians make tear analysis still challenging. Here, flexible surface-enhanced Raman scattering (SERS) substrates composed of gold nanoparticles (AuNPs) and two-dimensional MXene Ti3C2TX nanosheets have been designed. The GMXeP (gold nanoparticles with MXene nanosheets loaded on paper) SERS substrates show good sensitivity, reproducibility, and stability, yielding an enhancement factor (EF) of 3.7 × 105 at the concentration of 10-9 M. The GMXeP SERS substrates are used to detect glucose of diabetic tears within a linear range of 1-50 µM, the lowest detection concentration is 0.39 µM and the significant correlation between tear glucose and blood glucose indicates that this method is suitable for sensitive and noninvasive detection of blood glucose.


Assuntos
Diabetes Mellitus , Nanopartículas Metálicas , Glucose , Ouro , Humanos , Reprodutibilidade dos Testes , Análise Espectral Raman
2.
Front Genet ; 12: 730519, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34777467

RESUMO

Illumina is the leading sequencing platform in the next-generation sequencing (NGS) market globally. In recent years, MGI Tech has presented a series of new sequencers, including DNBSEQ-T7, MGISEQ-2000 and MGISEQ-200. As a complex application of NGS, cancer-detecting panels pose increasing demands for the high accuracy and sensitivity of sequencing and data analysis. In this study, we used the same capture DNA libraries constructed based on the Illumina protocol to evaluate the performance of the Illumina Nextseq500 and MGISEQ-2000 sequencing platforms. We found that the two platforms had high consistency in the results of hotspot mutation analysis; more importantly, we found that there was a significant loss of fragments in the 101-133 bp size range on the MGISEQ-2000 sequencing platform for Illumina libraries, but not for the capture DNA libraries prepared based on the MGISEQ protocol. This phenomenon may indicate fragment selection or low fragment ligation efficiency during the DNA circularization step, which is a unique step of the MGISEQ-2000 sequence platform. In conclusion, these different sequencing libraries and corresponding sequencing platforms are compatible with each other, but protocol and platform selection need to be carefully evaluated in combination with research purpose.

3.
World J Pediatr ; 2021 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-34787828

RESUMO

BACKGROUND: The clinical use of gentamicin always lies in its antimicrobial activity in the past as an aminoglycoside antibiotic. However, in the past decade, there were considerable interests in therapeutic approaches in treating hereditary diseases. Some of the genodermatosis is caused by nonsense mutations that create premature termination codons and lead to the production of truncated or non-functional proteins. Gentamicin could induce readthrough of nonsense mutations and enable the synthesis of full-length proteins. We focus on previous publications on topical application of gentamicin and review its utility in genetic skin diseases. DATA SOURCES: We search the MEDLINE through PubMed, EMBASE databases, and the Clinical Trials Registry Platform from January 1960 to July 2020 using the key search terms "gentamicin, topical gentamicin, genodermatosis, genetic skin diseases". RESULTS: The application of gentamicin in genodermatosis yielded promising results, both in vivo and in vitro, including Nagashima-type palmoplantar keratosis, epidermolysis bullosa, Hailey-Hailey disease, hereditary hypotrichosis simplex of the scalp, etc. CONCLUSIONS: Topical gentamicin is a potential treatment option for genodermatosis caused by nonsense mutation.

4.
Front Plant Sci ; 12: 705021, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34795680

RESUMO

Multi-target recognition and positioning using robots in orchards is a challenging task in modern precision agriculture owing to the presence of complex noise disturbance, including wind disturbance, changing illumination, and branch and leaf shading. To obtain the target information for a bud-cutting robotic operation, we employed a modified deep learning algorithm for the fast and precise recognition of banana fruits, inflorescence axes, and flower buds. Thus, the cutting point on the inflorescence axis was identified using an edge detection algorithm and geometric calculation. We proposed a modified YOLOv3 model based on clustering optimization and clarified the influence of front-lighting and backlighting on the model. Image segmentation and denoising were performed to obtain the edge images of the flower buds and inflorescence axes. The spatial geometry model was constructed on this basis. The center of symmetry and centroid were calculated for the edges of the flower buds. The equation for the position of the inflorescence axis was established, and the cutting point was determined. Experimental results showed that the modified YOLOv3 model based on clustering optimization showed excellent performance with good balance between speed and precision both under front-lighting and backlighting conditions. The total pixel positioning error between the calculated and manually determined optimal cutting point in the flower bud was 4 and 5 pixels under the front-lighting and backlighting conditions, respectively. The percentage of images that met the positioning requirements was 93 and 90%, respectively. The results indicate that the new method can satisfy the real-time operating requirements for the banana bud-cutting robot.

5.
Research (Wash D C) ; 2021: 9802795, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34738087

RESUMO

We report a novel Mn-Co-Ni-O (MCN) nanocomposite in which the p-type semiconductivity of Mn-Co-Ni-O can be manipulated by addition of graphene. With an increase of graphene content, the semiconductivity of the nanocomposite can be tuned from p-type through electrically neutral to n-type. The very low effective mass of electrons in graphene facilitates electron tunneling into the MCN, neutralizing holes in the MCN nanoparticles. XPS analysis shows that the multivalent manganese ions in the MCN nanoparticles are chemically reduced by the graphene electrons to lower-valent states. Unlike traditional semiconductor devices, electrons are excited from the filled graphite band into the empty band at the Dirac points from where they move freely in the graphene and tunnel into the MCN. The new composite film demonstrates inherent flexibility, high mobility, short carrier lifetime, and high carrier concentration. This work is useful not only in manufacturing flexible transistors, FETs, and thermosensitive and thermoelectric devices with unique properties but also in providing a new method for future development of 2D-based semiconductors.

6.
BMC Immunol ; 22(1): 72, 2021 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-34749650

RESUMO

Macrophages are involved in the pathophysiology of many diseases as critical cells of the innate immune system. Pyroptosis is a form of macrophage death that induces cytokinesis of phagocytic substances in the macrophages, thereby defending against infection. Dimethyl itaconate (DI) is an analog of itaconic acid with anti-inflammatory effects. However, the effect of dimethyl itaconate on macrophage pyroptosis has not been elucidated clearly. Thus, the present study aimed to analyze the effect of DI treatment on a macrophage pyroptosis model (Lipopolysaccharide, LPS + Adenosine Triphosphate, ATP). The results showed that 0.25 mM DI ameliorated macrophage pyroptosis and downregulated interleukin (IL)-1ß expression. Then, real-time quantitative polymerase chain reaction (RT-qPCR) was used to confirm the result of RNA-sequencing of the upregulated oxidative stress-related genes (Gclc and Gss) and downregulated inflammation-related genes (IL-12ß and IL-1ß). In addition, Gene Ontology (GO) enrichment analysis showed that differential genes were associated with transcript levels and DNA replication. Kyoto encyclopedia of genes and genomes (KEGG) enrichment showed that signaling pathways, such as tumor necrosis factor (TNF), Jak, Toll-like receptor and IL-17, were altered after DI treatment. N-acetyl-L-cysteine (NAC) reversed the DI effect on the LPS + ATP-induced macrophage pyroptosis and upregulated the IL-1ß expression. Oxidative stress-related protein Nrf2 is involved in the DI regulation of macrophage pyroptosis. Taken together, these findings suggested that DI alleviates the pyroptosis of macrophages through oxidative stress.

7.
Front Med (Lausanne) ; 8: 697027, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34671613

RESUMO

Background: Serum bilirubin level has been suggested to be associated with mortality for patients with severe sepsis. This study aimed to investigate the association of serum total bilirubin level with hospital mortality rate in adult critically ill patients. Method: Data were extracted from the Medical Information Mart for Intensive Care-III (MIMIC-III) database. Patients with measured serum total bilirubin levels that recorded within 24 h after admission were involved in this study. Association of serum total bilirubin level and hospital mortality rate was assessed using logistic regression analysis. Propensity score-matching (PSM) was used to minimize differences between different groups. Results: A total of 12,035 critically ill patients were herein involved. In patients with serum total bilirubin level ≥ 2 mg/dL, the hospital mortality rate was 31.9% compared with 17.0% for patients with serum total bilirubin level < 2 mg/dL (546/1714 vs. 1750/10321, P < 0.001). The results of multivariable logistic regression analysis showed that the odds ratio of mortality in patients with serum total bilirubin level ≥ 2 mg/dL was 1.654 [95% confidence interval (CI): 1.307, 2.093, P < 0.001]. After propensity score matching, in patients with serum total bilirubin level ≥ 2 mg/dL, the weighted hospital mortality rate was 32.2% compared with 24.8% for patients with serum total bilirubin level < 2 mg/dL, P = 0.001). Conclusions: Serum total bilirubin concentration was found to be independently associated with hospital mortality rate in adult critically ill patients.

8.
Ecotoxicol Environ Saf ; 226: 112836, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34601266

RESUMO

Di-(2-ethylhexyl) phthalate (DEHP), an extensively used plasticizer, can cause environmental pollution and organ injury. Lycopene (LYC) is a natural carotene that has the potential to prevent chronic diseases. To reveal the effect of DEHP and/or LYC on the kidney, male mice were treated with LYC (5 mg/kg) and/or DEHP (500 mg/kg or 1000 mg/kg) by gavage for 28 days. The study indicated that DEHP caused glomerular atrophy, tubular expansion, disappearance of the mitochondrial membrane, and cristae rupture. DEHP exposure can increase the expression of aquaporin (AQP) subunits and the activity of Ca2+-Mg2+-ATPase and decrease the activity of Na+-K+-ATPase, which results in ion disorder. However, LYC can relieve kidney injury by regulating the activity of ATPase, the expression of ATPase subunits, and AQP subunit expression. The results indicated that AQP was a target for LYC in antagonizing the disturbance of DEHP-induced renal damage.


Assuntos
Aquaporinas , Dietilexilftalato , Animais , Dietilexilftalato/toxicidade , Homeostase , Rim , Licopeno , Masculino , Camundongos
9.
Materials (Basel) ; 14(20)2021 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-34683670

RESUMO

Ultrahigh molecular weight polyethylene (UHMWPE) materials have been prevalent joint replacement materials for more than 45 years because of their excellent biocompatibility and wear resistance. In this study, functionalized activated nanocarbon (FANC) was prepared by grafting maleic anhydride polyethylene onto acid-treated activated nanocarbon. A novel porous UHMWPE composite was prepared by incorporating the appropriate amount of FANC and pore-forming agents during the hot-pressing process for medical UHMWPE powder. The experimental results showed that the best prepared porous UHMWPE/FANC exhibited appropriate tensile strength, porosity, and excellent hydrophilicity, with a contact angle of 65.9°. In vitro experiments showed that the porous UHMWPE/FANC had excellent biocompatibility, which is due to its porous structure and hydrophilicity caused by FANC. This study demonstrates the potential viability for our porous UHMWPE/FANC to be used as cartilage replacement material for biomedical applications.

10.
BMC Public Health ; 21(1): 1885, 2021 10 19.
Artigo em Inglês | MEDLINE | ID: mdl-34663285

RESUMO

BACKGROUND: China has introduced a series of stricter policies to criminalize drunk driving and increase penalties since May 2011. However, there is no previous study examining the time-varying impacts of drunk driving regulations on road traffic fatalities based on daily data. METHODS: We collected 6536 individual data of road traffic deaths (RTDs) in Guangzhou from 2008 to 2018. The quasi-Poisson regression models with an inclusion of the intervention variable and the interaction of intervention variable and a function of time were used to quantify the time-varying effects of these regulations. RESULTS: During the 11-year study period, the number of population and motor vehicles showed a steady upward trend. However, the population- and motor vehicles- standardized RTDs rose steadily before May 2011, the criminalizing drunk driving intervention was implemented and gradually declined after that. The new drunk driving intervention were associated with an average risk reduction of RTDs (ER = -9.01, 95% eCI: - 10.05% to - 7.62%) during the 7.7 years after May 2011. On average, 75.82 (95% eCI, 54.06 to 92.04) RTDs per 1 million population annually were prevented due to the drunk driving intervention. CONCLUSION: These findings would provide important implications for the development of integrated intervention measures in China and other countries attempting to reduce traffic fatalities by stricter regulations on drunk driving.


Assuntos
Dirigir sob a Influência , Acidentes de Trânsito/prevenção & controle , China/epidemiologia , Humanos , Análise de Séries Temporais Interrompida , Veículos Automotores
11.
Microb Ecol ; 2021 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-34714368

RESUMO

Host genotype and environment are considered crucial factors in shaping Daphnia gut microbiome composition. Among the environmental factors, diet is an important factor that regulates Daphnia microbiome. Most of the studies only focused on the use of axenic diet and non-sterile medium to investigate their effects on Daphnia microbiome. However, in natural environment, Daphnia diets such as phytoplankton are associated with microbes and could affect Daphnia microbiome composition and fitness, but remain relatively poorly understood compared to that of axenic diet. To test this, we cultured two Daphnia magna genotypes (genotype-1 and genotype-2) in sterile medium and fed with axenic diet. To check the effects of algal diet-associated microbes versus free water-related microbes, Daphnia were respectively inoculated with three different inoculums: medium microbial inoculum, diet-associated microbial inoculum, and medium and diet-mixed microbial inoculum. Daphnia were cultured for 3 weeks and their gut microbiome and life history traits were recorded. Results showed that Daphnia inoculated with medium microbial inoculum were dominated by Comamonadaceae in both genotypes. In Daphnia inoculated with mixed inoculum, genotype-1 microbiome was highly changed, whereas genotype-2 microbiome was slightly altered. Daphnia inoculated with diet microbial inoculum has almost the same microbiome in both genotypes. The total number of neonates and body size were significantly reduced in Daphnia inoculated with diet microbial inoculum regardless of genotype compared to all other treatments. Overall, this study shows that the microbiome of Daphnia is flexible and varies with genotype and diet- and medium-associated microbes, but not every bacteria is beneficial to Daphnia, and only symbionts can increase Daphnia performance.

12.
J Cancer ; 12(20): 6058-6070, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34539879

RESUMO

Cholangiocarcinoma (CCA) leads to poor prognosis due to high aggressiveness and common chemoresistance. Dihydromyricetin (DMY), the main bioactive compound isolated from Ampelopsis grossedentata, exhibits broad anti-tumor effects. This study aimed to investigate the inhibitory effect of DMY on CCA tumor growth and epithelial-mesenchymal transition (EMT) and its underlying mechanism in CCA. DMY treatment significantly inhibited cell proliferation and EMT in CCA cell lines. The expression of ZEB1 and vimentin were down-regulated, while the level of E-cadherin was increased after DMY treatment. By analyzing the TCGA dataset, we found that miR-455 expression was significantly downregulated, while the level of ZEB1 was up-regulated in human CCA tumor tissues compared to normal samples. Mechanistic studies showed that ZEB1 was a direct target of miR-455-3p in CCA. Moreover, DMY treatment potently increased miR-455-3p expression and inhibited ZEB1 expression. Inhibition of miR-455-3p expression abolished DMY's inhibitory effects on tumor growth and EMT in both CCA cells and cell-engrafted nude mice. Finally, DMY significantly suppressed the expressions of p-PI3K and p-AKT, while silencing miR-455-3p remarkably abrogated the inhibitory effect. In conclusion, DMY suppresses tumor growth and EMT through regulating miR-455-3p in human cholangiocarcinoma, suggesting a potential option for CCA treatment.

13.
Environ Pollut ; 291: 118143, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34517177

RESUMO

Driven by global warming and eutrophication, Microcystis blooms have posed a severe threat to freshwater ecosystems, especially their derived pollutants cause serious harm to aquatic organisms, thus it is urgent to develop an effective strategy to eliminate nuisance Microcystis. Some protozoa can efficiently graze on toxic Microcystis aeruginosa and degrade cyanotoxins, and play a vital role in regulating harmful cyanobacteria. In the process of protozoa feeding on harmful algae, both temperature and protozoa population density are critical factors that affect the consequences of harmful M. aeruginosa population dynamics. In this study, we first found that Paramecium multimicronucleatum has strong ability to feed on M. aeruginosa, and then studied the interactive effects between temperature and initial density of P. multimicronucleatum on controlling M. aeruginosa. Results showed that increasing temperature accelerated the elimination of M. aeruginosa by P. multimicronucleatum, e.g. the time for M. aeruginosa elimination at 32 °C was shortened to 3.5-4 days. The higher temperatures (26, 29, and 32 °C) were more conducive to improve the efficiency of controlling M. aeruginosa by P. multimicronucleatum with low initial density (10 inds mL-1). Furthermore, P. multimicronucleatum can rapidly degrade microcystins, and the degradation ratio approximately 100% at 32 °C after 6 days. This is the first study to discover that P. multimicronucleatum can high efficiently graze on M. aeruginosa and has a much higher grazing rate (3.5-5.5 × 104Microcystis Paramecium-1 d-1) than other protozoa. These findings contribute to the establishment of a new feasible method for the biological control of M. aeruginosa, and provide a theoretical guidance for the practical application of P. multimicronucleatum in the removal of M. aeruginosa.


Assuntos
Microcystis , Paramecium , Ecossistema , Microcistinas , Temperatura
14.
J Oncol ; 2021: 5512325, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34367284

RESUMO

Aims: Lung adenocarcinoma (LUAD) cells could escape from the monitoring of immune cells and metastasize rapidly through immune escape. Therefore, we aimed to develop a method to predict the prognosis of LUAD patients based on immune checkpoints and their associated genes, thus providing guidance for LUAD treatment. Methods: Gene sequencing data were downloaded from the Cancer Genome Atlas (TCGA) and analyzed by R software and R Bioconductor software package. Based on immune checkpoint genes, kmdist clustering in ConsensusClusterPlus R software package was utilized to classify LUAD. CIBERSORT was used to quantify the abundance of immune cells in LUAD samples. LM22 signature was performed to distinguish 22 phenotypes of human infiltrating immune cells. Gene set variation analysis (GSVA) was performed on immune checkpoint cluster and immune checkpoint score using GSVA R software package. The risk score was calculated by LASSO regression coefficient. Gene Ontology (GO), Hallmark, and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed. PROC was performed to generate the ROC curve and calculate the area under the curve (AUC). Results: According to the immune checkpoint, LUAD was classified into clusters 1 and 2. Survival rate, immune infiltration patterns, TMB, and immune score were significantly different between the two clusters. Functional prediction showed that the functions of cluster 1 focused on apoptosis, JAK/STAT signaling pathway, TNF-α/NFκB signaling pathway, and STAT5 signaling pathway. The risk score model was constructed based on nine genes associated with immune checkpoints. Survival analysis and ROC analysis showed that patients with high-risk score had poor prognosis. The risk score was significantly correlated with cancer status (with tumor), male proportion, status, tobacco intake, and cancer stage. With the increase of the risk score, the enrichment of 22 biological functions increased, such as p53 signaling pathway. The signature was verified in IMvigor immunotherapy dataset with excellent diagnostic accuracy. Conclusion: We established a nine-gene signature based on immune checkpoints, which may contribute to the diagnosis, prognosis, and clinical treatment of LUAD.

15.
Asian J Surg ; 2021 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-34426060

RESUMO

BACKGROUND: CTCs play a critical role in the diagnosis and prognosis of liver cancer. However, there are few studies on whether different types of CTCs can predict the prognosis in patients with HCC following LT. METHODS: Retrospective data including CTCs detected by the CanPatrolTM platform combined with RNA-ISH were collected and analyzed on 56 patients from December 2016 to December 2019 at the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, China. RESULTS: During the study period, fifty-six patients (51 males, 5 females) were included with an mean age of 52 ± 9 years. The 1-, 2- and 3-year recurrence rates of postoperative interstitial CTC-positive and CTC-negative groups were 21.7% vs 10.8%, 37.5% vs 10.8% and 55.5% vs 10.8%, confirming a statistically significant difference between the 2 groups (p = 0.044). The 1-, 2- and 3-year recurrence rates of the increasing interstitial CTCs group were 25.2%, 36.9% and 66.9%, while 12.6%, 24.4% and 24.4% in the decreasing and unchanged group, indicating a significant difference (p = 0.038). CONCLUSION: CanPatrolTM platform presents a superior analytical sensitivity, and may be used as a dynamic monitoring tool for CTCs. And interstitial CTCs which are more aggressive and metastatic caused by EMT can be regarded as a predictor of post-transplant tumor recurrence after LT for HCC.

16.
Angew Chem Int Ed Engl ; 60(44): 23578-23583, 2021 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-34423529

RESUMO

Chiral Pb(Sn)-I hybrid organic-inorganic perovskites exhibit outstanding chiral-induced spin selectivity (CISS) performance, but the nontoxic lead-free hybrid materials with high stability are still greatly desired for spin filtering in spintronic applications. We synthesize chiral hybrid copper halides (R/S-MBA)2 CuX4 (MBA=methylbenzylammonium; X=Cl, Br) with characteristic 0D CuX4 tetrahedral structural motifs, combining the low toxicity of Cu2+ and air stability of halide ions (Cl- and Br- ). Despite similar structural and electronic features, (R/S-MBA)2 CuBr4 shows much smaller chiroptical activity than the chloride counterpart. Magnetically conductive atomic force microscopy measurements display a typical spin-polarized charge-transport property with high efficiency up to 90 % for both copper halides. Our work expands the CISS effect into eco-friendly and stable metal-organic halides, which is promising for applications in spintronics based on transition-metal hybrid systems.

17.
Transplant Proc ; 53(7): 2133-2139, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34426023

RESUMO

BACKGROUND: Dimethyl fumarate (DMF) is a novel antioxidant that selectively reduces hydroxyl radicals. This study aimed to investigate the potential role of DMF in the pathogenesis of renal ischemia-reperfusion injury (IRI) and the mechanisms involved. METHODS: C57BL/6 wild-type mice were treated with DMF or a vehicle. Subsequently, renal IRI was induced in mice by a model of right kidney nephrectomy and left renal ischemia for 30 minutes followed by reperfusion for 24 hours. Sham operation and phosphate-buffered saline were used as controls. Serum and renal tissues were collected at 24 hours after IRI to evaluate the influence of DMF on the recovery of renal function after IRI. Blood urea nitrogen and serum creatinine levels were measured. Kidney cell apoptosis was evaluated using terminal deoxynucleotidyl transferase dUTP nick end labeling-positive staining. Interleukin 6 and tumor necrosis factor α cytokines in the kidney tissues were measured. Indicators of oxidative stress in the kidneys were detected. Finally, Nrf2-deficient mice were used to determine the protective role of the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) and NAD(P)H dehydrogenase quinone 1 (NQO1) signaling pathways induced by DMF using western blot assay. RESULTS: DMF significantly attenuated renal dysfunction in mice and showed reductions in the severity of renal tubular injury, cell necrosis, and apoptosis. Moreover, DMF significantly reduced the amount of key inflammatory mediators. Additionally, DMF attenuated the malondialdehyde levels 24 hours after IRI but upregulated the superoxide dismutase activities. Western blot assay showed that DMF significantly increased the protein levels of Nrf2, HO-1, and NQO-1. Importantly, these DMF-mediated beneficial effects were not observed in Nrf2-deficient mice. CONCLUSIONS: DMF attenuates renal IRI by reducing inflammation and upregulating the antioxidant capacity, which may be through Nrf2/HO-1and NQO1 signaling pathway.


Assuntos
Fator 2 Relacionado a NF-E2 , Traumatismo por Reperfusão , Animais , Apoptose , Fumarato de Dimetilo/metabolismo , Fumarato de Dimetilo/farmacologia , Modelos Animais de Doenças , Rim/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Fator 2 Relacionado a NF-E2/metabolismo , Estresse Oxidativo , Traumatismo por Reperfusão/metabolismo , Traumatismo por Reperfusão/prevenção & controle
18.
Ecotoxicol Environ Saf ; 222: 112498, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34265527

RESUMO

As the major constituents of PM2.5, carbonaceous constituents and inorganic ions have attracted emerging attentions on their health risks, particularly on cardiorespiratory diseases. However, evidences on the risks of PM2.5 constituents on other diseases (eg. nervous disease, genitourinary disease, neoplasms and endocrine disease) remain scarce. In our study, we firstly calculated residuals of PM2.5 constituents regressed on PM2.5 to remove the confounding effect of PM2.5. Then, generalized additive model (GAM) was used to assess impacts of residuals of PM2.5 constituents on mortality from 36 diseases (10 broad categories and 26 subcategories) during 2011-2015 in Guangzhou, China. Results of constituent-residual models showed that only EC, OC and NO3- were significantly associated with all-cause mortality, with per IQR change in corresponding constituent residuals related to percentage changes of 1.69% (95% CI: 0.42, 2.97), 1.94% (95% CI: 0.37, 3.54) and 2.59% (95% CI: 1.02, 4.18) at lag 03 days. All these pollutants were significantly associated with elevated mortality risk of cardiovascular disease, but only EC was significantly associated with respiratory mortality, and NO3- with endocrine disease and neoplasm. For more specific causes, the highest effect estimates of EC and NO3-were both observed on mortality from other form of heart disease, and OC on intentional self-harm, with estimates of 11.45% (95% CI: 2.74, 20.91), 12.59% (95% CI: 1.41, 25.02) and 18.01% (95% CI: 2.14, 36.36), respectively. Our findings highlighted that stricter emission control measures are still warranted to reduce air pollution level and protect the public health.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Doenças Cardiovasculares , Poluentes Atmosféricos/análise , Poluentes Atmosféricos/toxicidade , Poluição do Ar/efeitos adversos , Poluição do Ar/análise , Doenças Cardiovasculares/induzido quimicamente , China/epidemiologia , Humanos , Material Particulado/análise , Material Particulado/toxicidade
19.
DNA Cell Biol ; 40(9): 1200-1210, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34227876

RESUMO

In vitro generation of red blood cells has the potential to circumvent shortfalls in the global demand for blood for transfusion applications. However, cell differentiation and proliferation are often regulated by precise changes in gene expression, but the underlying mechanisms and molecular changes remain unclear. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) can be used to evaluate multiple target genes. To make the results more reliable, suitable reference genes should be used to calibrate the error associated with qRT-PCR. In this study, we utilized bioinformatics to screen 3 novel candidate reference genes (calcium and integrin binding family member 2 [CIB2], olfactory receptor family 8 subfamily B member 8 [OR8B8], and zinc finger protein 425 [ZNF425]) along with eight traditional reference genes (glyceraldehyde-3-phosphate dehydrogenase [GAPDH], ß-actin [ACTB], 18S RNA, ß2-microglobulin [ß2-MG], peptidylprolyl isomerase A [PPIA], TATA box-binding protein [TBP], hydroxymethylbilane synthase [HMBS], and hypoxanthine phosphoribosyltransferase 1 [HPRT1]). Two software algorithms (geNorm and NormFinder) were used to evaluate the stability of expression of the 11 genes at different stages of erythrocyte development. Comprehensive analysis showed that expression of GAPDH and TBP was the most stable, whereas ZNF425 and OR8B8 were the least suitable candidate genes. These results suggest that appropriate reference genes should be selected before performing gene expression analysis during erythroid differentiation and that GAPDH and TBP are suitable reference genes for gene expression studies on erythropoiesis.


Assuntos
Proteínas de Ligação ao Cálcio/sangue , Eritrócitos/metabolismo , Proteínas Repressoras/sangue , Células-Tronco/metabolismo , Antígenos CD34/metabolismo , Biomarcadores/sangue , Proteínas de Ligação ao Cálcio/metabolismo , Diferenciação Celular , Eritrócitos/citologia , Sangue Fetal/metabolismo , Expressão Gênica , Humanos , Células-Tronco/citologia
20.
J Environ Manage ; 297: 113281, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34274765

RESUMO

Eutrophication of natural water bodies worldwide has led to cyanobacteria becoming the dominant species in phytoplankton communities, causing serious harm environmentally and economically. Cyanobacterial succession makes effective treatment of cyanobacterial blooms a challenge. Although there are many studies about cyanobacterial dominance and succession, it is still lack of relevant review summarizing the advances on this topic. To control cyanobacterial blooms and manage water quality effectively, we conducted a critical review and drew the following conclusions: (1) cyanobacterial dominance and succession occur from spring to summer, with changes of multiple environmental factors dominated by temperature and nutrients conditions; (2) the cyanobacterial dominance and succession are inherently attributed to the distinctive traits of cyanobacteria including colony formation, gas vesicles, toxin release, and nitrogen fixation; (3) given the current meta-omics explorations on mechanisms of cyanobacterial succession, how to combine the extensive data to draw general conclusions is a challenge in the future; (4) the dominant niche of high temperature-adapted cyanobacteria genera will be further reinforced with global warming and elevated carbon dioxide in the future; (5) considering the causes and future developments of cyanobacterial blooms, the management strategies for controlling cyanobacterial blooms include reducing external nutrient input and removing internal nutrient in sediment, artificial mixing waters to decrease buoyancy of cyanobacteria, and biological control using allelopathy of aquatic plants and/or enhancing zooplankton feeding.


Assuntos
Cianobactérias , Animais , Eutrofização , Aquecimento Global , Lagos , Fitoplâncton , Zooplâncton
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