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1.
J Mol Neurosci ; 2021 Dec 23.
Artigo em Inglês | MEDLINE | ID: mdl-34939148

RESUMO

AIF-1 gene is surrounded by the genes involved in the inflammatory response and located in the major histocompatibility complex (MHC) class III genomic region. It has been found that microglial cells expressed the AIF-1 gene during all stages of mice brain development. However, the role of AIF-1 remains unclear in glioma. A total of 1270 glioma patients from three independent data sets were enrolled in the study. TIMER platform was used for comprehensive molecular characterization of tumor immune infiltrates. Sangerbox was used to analyze AIF-1 RNA sequencing expression data of tumors and normal samples, and to evaluate the association between AIF-1 expression and 29 sub-populations of immune cells. The R language 3.63 was used to identify differentially expressed genes for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment. Kaplan-Meier survival analysis and univariate/multivariate Cox analysis were used to examine survival distributions. We found that AIF-1 gene was prominently up-regulated, especially in brain glioma including LGG and GBM. A strong correlation was observed between AIF-1 expression and the majority of immune cells, particularly in macrophage, myeloid-derived suppressor cells. Moreover, AIF-1 expression was correlated with immune infiltration level. We found that AIF-1 expression was strongly correlated with the specific immune and prognostic cell markers of monocytes, microglia and macrophages, M1 macrophages, and M2 macrophages after normalization through tumor purity in TCGA-LGG and TCGA-GBM. Higher expression level of AIF-1 was found to be significantly correlated with poor prognosis. GO analysis and KEGG pathways indicated that AIF-1 could affect glioma-related immune activities. Our study suggests that AIF-1 can be treated as a prognostic biomarker for glioma patients. AIF-1 was involved in pro-tumor processes and the regulation of immune status and correlates with poor prognosis.

2.
J Agric Food Chem ; 69(47): 14065-14074, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34775748

RESUMO

Mitochondria play an essential role in maintaining cellular metabolic homeostasis. However, its dysfunction will cause different pathophysiological consequences. A specific mechanism of action has been developed by cells to adapt to changes in physiological conditions or in response to different stimuli, by meditating mitochondrial number, structure, and energy metabolism. Whole grains are considered healthier than refined grains for their higher amounts of bioactive components, with proven multiple health benefits. The modulation of an appropriate mitochondrial function contributes to the bioactive-component-based health improvements. Thus, this review aims to represent current studies that identify the impact of natural bioactive components in whole grains against metabolic disorders by modulating mitochondrial biogenesis and energy metabolism. It seems most attractive to aim nutritional intervention at the prevention or treatment of metabolic abnormalities and hence to target dietary management at improvement of mitochondrial function.

3.
J Agric Food Chem ; 69(45): 13373-13385, 2021 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-34735146

RESUMO

Succinate dehydrogenase (SDH) is known as an ideal target for the investigations of fungicides. To develop novel SDH inhibitors, 30 novel thiophene/furan-1,3,4-oxadiazole carboxamide derivatives were designed and synthesized. In the in vitro antifungal assay, a majority of the target compounds demonstrated fair to potent antifungal activity against seven tested phytopathogenic fungi. Compounds 4b, 4g, 4h, 4i, and 5j showed remarkable antifungal activity against Sclerotinia sclerotiorum, affording EC50 values ranging from 0.1∼1.1 mg/L. In particular, compound 4i displayed the most potent activity against S. sclerotiorum (EC50 = 0.140 ± 0.034 mg/L), which was superior to that of boscalid (EC50 = 0.645 ± 0.023 mg/L). A further morphological investigation revealed the abnormal mycelia and damaged cell structures of compound 4i-treated S. sclerotiorum by scanning electron microscopy. Furthermore, the in vivo antifungal assay against S. sclerotiorum revealed that compounds 4g and 4i were effective for suppressing rape Sclerotinia rot at a dosage of 200 mg/L. In the SDH inhibition assay, compounds 4g and 4i also presented significant inhibitory activity with IC50 values of 1.01 ± 0.21 and 4.53 ± 0.19 µM, respectively, which were superior or equivalent to that of boscalid (3.51 ± 2.02 µM). Molecular docking and molecular dynamics simulation of compound 4g with SDH revealed that compound 4g could form strong interactions with the key residues of the SDH. These results indicated that this class of derivatives could be a promising scaffold for the discovery and development of novel SDH inhibitors.


Assuntos
Fungicidas Industriais , Succinato Desidrogenase , Antifúngicos/farmacologia , Ascomicetos , Inibidores Enzimáticos/farmacologia , Fungicidas Industriais/farmacologia , Furanos/farmacologia , Simulação de Acoplamento Molecular , Oxidiazóis , Relação Estrutura-Atividade , Succinato Desidrogenase/metabolismo , Ácido Succínico , Tiofenos/farmacologia
4.
Chem Biodivers ; : e2100651, 2021 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-34626068

RESUMO

In order to find novel potential antifungal agrochemicals, a series of new 4-(1,2,4-oxadiazol-3-yl)-N-(4-phenoxyphenyl)benzamide derivatives 3a-j were designed, synthesized and characterized by their 1 H-, 13 C-NMR and HRMS spectra. The preliminary antifungal assay in vitro revealed that compounds 3a-j exhibited moderate to good antifungal activity against five plant pathogenic fungi. Especially, compound 3e presented significant antifungal activity against Alternaria solani, Botrytis cinerea and Sclerotinia sclerotiorum, superior to positive control boscalid. In the in vivo antifungal assay on tomato plants and cucumber leaves, compound 3e presented good inhibition rate against B. cinerea at 200 mg/L. Molecular dynamics simulation revealed that compound 3e could bind with the active site of class II histone deacetylase (HDAC).

5.
Talanta ; 234: 122674, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34364474

RESUMO

Ambient ionization of glycans is simply and efficiently achieved by spraying from an alkali metal salt-impregnated paper surface. Monosaccharides, oligosaccharides and ring glycans easily form abundant alkali metal adduct ions, and give simple and clean high-quality mass spectra. The enhancement is specific for glycans, compared to a wide variety of non-glycan compounds present in a matrix. In addition, molecular weight of unknown glycans can be further identified based on the ion mass difference of various alkali metal adduct ions from a certain compound when using a mixed salt-impregnated paper containing five cation salts. Successful determination of glycans and glycoconjugates in plant extracts, honey, blood and urine demonstrates the practicability of this approach to complicated matrices, especially biological matrices.


Assuntos
Metais Alcalinos , Polissacarídeos , Peso Molecular , Oligossacarídeos , Espectrometria de Massas por Ionização por Electrospray , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
6.
Hortic Res ; 8(1): 89, 2021 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-33828071

RESUMO

Hemerocallis citrina Borani (huang hua cai in Chinese) is an important horticultural crop whose flower buds are widely consumed as a delicious vegetable in Asia. Here we assembled a high-quality reference genome of H. citrina using single-molecule sequencing and Hi-C technologies. The genome assembly was 3.77 Gb and consisted of 3183 contigs with a contig N50 of 2.09 Mb, which were further clustered into 11 pseudochromosomes. A larger portion (3.25 Gb or 86.20%) was annotated as a repetitive content and 54,295 protein-coding genes were annotated in the genome. Genome evolution analysis showed that H. citrina experienced a recent whole-genome duplication (WGD) event at ~15.73 million years ago (Mya), which was the main factor leading to many multiple copies of orthologous genes. We used this reference genome to predict 20 genes involved in the rutin biosynthesis pathway. Moreover, our metabolomics data revealed neither colchicine nor its precursors in H. citrina, challenging the long-standing belief that this alkaloid causes poisoning by the plant. The results of our disruptive research are further substantiated by our genomic finding that H. citrina does not contain any genes involved in colchicine biosynthesis. The high-quality genome lays a solid foundation for genetic research and molecular breeding of H. citrina.

7.
Appl Opt ; 60(8): 2455-2464, 2021 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-33690347

RESUMO

Based on the acousto-optic effect, we propose a new method to directly measure water sound velocity that avoids the error-like phase ambiguity brought by the piezoelectric effect that is broadly adopted in current methods. In the experimental setup we designed, the laser signal modulated by the propagating acoustic wave changes its phase suddenly when the wave crosses the two or more intercepting laser lines simultaneously. This new design creatively realizes the possibility to capture time information at the phase level in sound velocity measurement, which is hardly realized in the piezoelectric-effect-based methods. Utilizing the above principle and the derived mathematical calculation, the accuracy of sound velocity with good traceability can be obtained. The experimental results show that the repeatability of the measurement results is less than 0.0159 m/s, and the accuracy compared with the commercial sound velocity profiler is better than 0.02 m/s.

8.
Food Sci Nutr ; 9(1): 489-498, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33473310

RESUMO

Alkylresorcinols (ARs) are important bioactive components in wheat bran which have been used as biomarkers for whole grain wheat consumption. In this study, the impact of ARs on the formation of Nε-(carboxymethyl)lysine (CML), the main component of dietary advanced glycation end products which could induce chronic disease was analyzed. Moreover, the influence of the addition of ARs on the sensory profiles of wheat bread was evaluated. ARs supplementation (0.03%, 0.1%, and 0.3% w/w) could significantly decrease the formation of CML by 21.70%, 35.11%, and 42.18%, respectively, compared with the control. Moreover, ARs-supplemented bread achieved a higher score in overall acceptability and buttery-like aroma through sensory evaluation. The volatile compounds in bread supplemented with ARs were characterized by headspace solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS), among which acetoin, 2,3-butanedione, 3-methyl-1-butanol, 2-phenylethanol, and 2-methylbutanal were confirmed as the main volatile compounds through determination of odor activity value. In addition, ARs supplementation had no negative impact on the chewiness, hardness, and springiness of bread. These findings demonstrated that ARs could be applied as potential food additives to improve the quality and sensory profile of bread.

9.
Front Oncol ; 10: 558414, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33178586

RESUMO

Background: Existing research shows that p-coumaric acid (p-CA) can inhibit the proliferation of a variety of tumor cells in vitro. However, there are no reports on the anti-tumor effects of p-CA on melanoma cells. In this study, the inhibitory effects of p-CA on mouse melanoma B16 and human melanoma A375 cells are reported, and the related mechanisms are investigated. Methods: CCK-8 assay was used to detect the effects of p-CA on cell vitality, colony formation assay was used to observe the effects on cell proliferation, Hoechst 33,258 staining was used to observe the morphology of apoptotic cells, flow cytometry was used to detect the effects on apoptosis and the cell cycle, and western blot was used to measure the levels of cell cycle- and apoptosis-related signaling pathway proteins. Results: p-CA significantly inhibits cell proliferation of A375 and B16 cells in a dose-dependent manner and obviously induced cell morphological changes. p-CA arrested A375 cells in the S phase by downregulating the cell cycle-related proteins Cyclin A and CDK2, and arrested B16 cells in the G0-G1 phase through downregulating the cell cycle-related proteins Cyclin E and CDK2. In addition, p-CA significantly promoted apoptosis of A375 and B16 cells. Furthermore, p-CA significantly upregulated the levels of Apaf1 and Bax and downregulated the levels of Bcl-2, and subsequently increased the levels of cytoplasmic cytochrome c (Cyto-c), cleaved caspase-3, and cleaved caspase-9, leading to apoptosis in A375 and B16 cells. Conclusion: p-CA can significantly inhibit the proliferation of human and mouse melanoma cells in vitro. Our research is a step in the development of anti-melanoma drugs.

10.
Reprod Biol ; 20(4): 580-583, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33191143

RESUMO

The aim of this study was to compare the efficiency of in vitro embryo production (IVP) following the collection of bovine ovaries and 22-h in vitro maturation (IVM) of oocytes in media prepared with Milli-Q® Water (n = 509 oocytes) or nanowater (NW; n = 304 oocytes). The mean cleavage (63.8 ± 4.6 % vs. 63.6 ± 6.1 %, respectively; mean ± SEM) and blastocyst formation rate (16.3 ± 3.4 % vs. 16.7 ± 6.7 % of presumptive zygotes, respectively) did not vary (P > 0.05; Student t-test) between the two types of media diluents. NW is a safe substitute for Milli-Q® Water for IVM of bovine oocytes.


Assuntos
Bovinos/embriologia , Técnicas de Cultura Embrionária/veterinária , Desenvolvimento Embrionário/fisiologia , Água , Animais , Blastocisto/fisiologia , Fase de Clivagem do Zigoto/fisiologia , Meios de Cultura , Técnicas de Cultura Embrionária/métodos , Feminino , Fertilização In Vitro/métodos , Fertilização In Vitro/veterinária , Técnicas de Maturação in Vitro de Oócitos/veterinária , Ovário/citologia
11.
Sci Rep ; 10(1): 20034, 2020 11 18.
Artigo em Inglês | MEDLINE | ID: mdl-33208868

RESUMO

Metabolic syndrome (MetS) is hitting high notes in the aging society in China. However, the prevalence and associated factors in Chinese aging population lack clarity to some extent. In the present study, we projected to inquire into the prevalence of MetS and its associated factors by analyzing datasets downloaded from the China Health and Retirement Longitudinal Study (CHARLS). Data comprising age, gender, socioeconomic status, lifestyle and health behaviors as well as blood biomarkers were subjected to descriptive statistics followed by univariate logistic regression and multivariate logistic regression. The overall prevalence of MetS was 33.38% (95% CI 32.42-34.34%). With age augments, prevalence increased during 40-70 years, while declined in participants aged 70 years above. Females had 2.94 times of risks (95% CI 2.55-3.39, P < 0.001). Marital status and alcohol consumption contributed nothing to the suffering of MetS. Participants with GDP per capita > 10,000 RMB and a non-agricultural hukou sustained higher risks than other participants (P < 0.05). Participants under education of middle school suffered 1.16 times of risks than other level of education (95% CI 1.01-1.34, P < 0.05). Smokers, participants with high low-density lipoprotein (LDL) or hyperuricemia or high glycosylated hemoglobin HbA1c sustained increased risks (P < 0.05). In Chinese aging population, with the augment of age, the prevalence ascended in men, while descended in women and was interfered by socioeconomic status, lifestyle and health behaviors as well as blood biomarkers, but not marital status and alcohol consumption.


Assuntos
Envelhecimento/patologia , Estilo de Vida , Síndrome Metabólica/epidemiologia , Fatores Socioeconômicos , Adulto , Idoso , China/epidemiologia , Escolaridade , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Fatores de Risco
12.
Molecules ; 25(20)2020 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-33096843

RESUMO

Using antibiotics as feed additives have been successively banned worldwide from 1986; therefore, it is an urgent task to finding safe and effective alternatives. As natural products of plant origin, essential oils (EOs) are an outstanding option due to their reported bioactivity. In this research, ten EOs of Labiatae species were extracted by steam distillation and its chemical constituents were identified by gas chromatography-mass spectrometry (GC-MS). A total of 123 chemical compounds, including alkenes, phenols, aldehydes and ketones, were identified. The results of antioxidant activity carried out through DPPH free radical scavenging (DPPH) and ferric reducing antioxidant power (FRAP), showing that EOs of Ocimum basilicum Linn. (ObEO), Thymus mongolicus Ronn. (TmEO), Origanum vulgare Linn. (OvEO) and Mosla chinensis Maxim. (McEO) have strong antioxidant activities. Their 50%-inhibitory concentration (IC50) value was <1.00, 1.42, 1.47 and 1.92 µg/mL, respectively; and their FRAP value was 1536.67 ± 24.22, 271.84 ± 4.93, 633.71 ± 13.14 and 480.66 ± 29.90, respectively. The results of filter paper diffusion showing that McEO, OvEO and TmEO inhibition zone diameter (IZD) are all over 30 mm. The results of two-fold dilution method showed that McEO, OvEO and TmEO have strong antibacterial activities against Staphylococcus aureus (S. aureus) and their minimal inhibitory concentrations (MIC) value was 1 µL/mL, 2 µL/mL, and 2 µL/mL, respectively. In conclusion, the results in this work demonstrate the possibility for development and application of EOs as potential feed additives.


Assuntos
Antibacterianos/farmacologia , Antioxidantes/farmacologia , Compostos de Bifenilo/antagonistas & inibidores , Lamiaceae/química , Óleos Voláteis/química , Picratos/antagonistas & inibidores , Staphylococcus aureus/efeitos dos fármacos , Aldeídos/química , Aldeídos/isolamento & purificação , Aldeídos/farmacologia , Alcenos/química , Alcenos/isolamento & purificação , Alcenos/farmacologia , Antibacterianos/química , Antibacterianos/isolamento & purificação , Antioxidantes/química , Antioxidantes/isolamento & purificação , Cetonas/química , Cetonas/isolamento & purificação , Cetonas/farmacologia , Testes de Sensibilidade Microbiana , Fenóis/química , Fenóis/isolamento & purificação , Fenóis/farmacologia
13.
J Acoust Soc Am ; 148(1): EL14, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-32752752

RESUMO

This study compares event-related potentials (ERPs) elicited by variations of sound location in free and reverberant fields. The virtual sound sources located at azimuths 0°-40° were synthesized with head-related transfer functions and binaural room impulse responses for free and reverberant fields, respectively. The sound stimulus at 0° was assigned as standard in the oddball paradigm. Results show that the P3 amplitude is larger in the free field and acoustical conditions have no significant effect on the amplitudes of N2 and mismatch negativity. Moreover, a linear relationship between sound angle and amplitude of ERP components is observed.


Assuntos
Localização de Som , Estimulação Acústica , Eletroencefalografia , Potenciais Evocados , Potenciais Evocados Auditivos , Som
14.
Food Funct ; 11(4): 3749, 2020 04 30.
Artigo em Inglês | MEDLINE | ID: mdl-32215432

RESUMO

Correction for '5-Heptadecylresorcinol attenuates oxidative damage and mitochondria-mediated apoptosis through activation of the SIRT3/FOXO3a signaling pathway in neurocytes' by Jie Liu et al., Food Funct., 2020, DOI: 10.1039/c9fo03028j.

15.
Food Funct ; 11(3): 2535-2542, 2020 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-32141452

RESUMO

5-Heptadecylresorcinol (AR-C17) is a main component of the alkylresorcinols (ARs), and has been widely used as a biomarker for whole grain rye consumption. In the present study, the neuroprotective effect and potential defense mechanisms of AR-C17 against hydrogen peroxide (H2O2)-induced apoptosis and mitochondrial dysfunction were investigated in PC-12 cells. The results showed that AR-C17 treatment significantly suppressed oxidative damage to cells, and ROS-mediated cells apoptosis. Furthermore, AR-C17 improved multiple mitochondrial bioactivities, reducing mitochondrial ROS levels, maintaining the mitochondrial membrane potential and enhancing mitochondrial respiration, leading to an elevation in the cellular ATP production, maximal respiration and spare respiratory capacity. In addition, AR-C17 exposure dramatically increased the protein expression of SIRT3 and its downstream functional gene FOXO3a. The cytoprotective effect of AR-C17 was abolished by the SIRT3 inhibitor 3-TYP, which led to increased cell apoptosis. Taken together, our findings demonstrate that AR-C17 could be used as a potential nutraceutical in reducing neuronal oxidative damage.


Assuntos
Apoptose/efeitos dos fármacos , Mitocôndrias/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Substâncias Protetoras/farmacologia , Resorcinóis/farmacologia , Animais , Proteína Forkhead Box O3/metabolismo , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Células PC12 , Ratos , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais/efeitos dos fármacos , Sirtuínas/metabolismo
16.
J Pestic Sci ; 45(1): 39-44, 2020 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-32110162

RESUMO

Six novel nicotinamide derivatives bearing a diarylamine-modified scaffold with flexible heterocyclic patterns were designed, synthesized, and characterized in detail via Hydrogen nuclear magnetic resonance (1H-NMR), Carbon nuclear magnetic resonance (13C-NMR), and Electrospray ionization mass spectrometry (ESI-MS). Their fungicidal activities and succinate dehydrogenase (SDH) enzymatic inhibitory abilities were evaluated. Preliminary fungicidal bioassay results showed that some of the target compounds exhibited moderate fungicidal activity. Among them, compound 4a showed 40.54% inhibition against Botrytis cinerea fungi. An SDH enzymatic inhibition assay revealed that the IC50 of compound 4b was 3.18 µM. This result indicated that the enzymatic inhibition level of 4b was similar to that of boscalid. Compound 4f exhibited superior comprehensive fungicidal and SDH enzymatic inhibitory activities. Molecular docking results suggested that 4f could bind well to the substrate cavity and the entrance cavity of SDH (1YQ3). In particular, 4f could react with the key catalytic site Arg 297. This phenomenon implied that 4f could act as the lead compound for further optimization.

17.
Phytochem Anal ; 31(2): 215-220, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31423695

RESUMO

INTRODUCTION: Proanthocyanidins have been widely developed and utilised in food, medicine, health care products and cosmetics. Porter-high-performance liquid chromatography (HPLC) is a quantitative method often utilised in many fields. This method uses a water bath to reflux the proanthocyanidins, but the process is cumbersome, the reagent consumption is large, and multiple batches of simultaneous treatments cannot be evaluated. OBJECTIVE: To establish a more convenient, rapid and high-batch processing method for determining the content of proanthocyanidins in functional food by HPLC. METHODS: N-Butanol-hydrochloric acid and iron salt are used as the reaction medium in the Porter method. After investigating the optimal conditions, the hydrolysis of proanthocyanidins can be performed in a microwave reactor at a power of 640 W for 75 s in the Porter reaction system. The content of proanthocyanidins was determined by HPLC with external standards. RESULTS: After the rapid pretreatment of samples, proanthocyanidins were determined by HPLC with a diode array detector at a detection wavelength of 525 nm with 0.53 µg/mL and 1.61 µg/mL limits of detection and quantification, respectively, for proanthocyanidin ions, and the linearity was 0.9999. Intra- and inter-day relative standard deviation values were between 1.5% and 3.1%, and the recovery was between 91.40% and 107.43% for the determination of different products, such as capsules, tablets and tea, which were similar to the values obtained with the conventional Porter method. CONCLUSION: This method is a time-saving and low cost approach for quantitative analysis of various proanthocyanidin health products that offers the advantages of high-batch processing and environmental friendliness.


Assuntos
Proantocianidinas , Cromatografia Líquida de Alta Pressão , Micro-Ondas
18.
J Cancer ; 10(24): 6065-6073, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31762816

RESUMO

The present study was aimed to investigate the role and mechanism of neurotrophin-3 (NT-3) and its specific receptor tropomyosin receptor kinase C (TrkC) in the perineural invasion (PNI) process of the salivary adenoid cystic carcinoma (SACC). The co-cultured system between SACC cells and Schwann cells (SCs) was employed to detect the expression of NT-3 and TrkC. The results of ELISA, qRT-PCR and western blot showed that NT-3 was noticeably elevated in the co-cultured SACC-83 cells, while TrkC was increased in the co-cultured SCs. The results of scratch wound healing, migration, and 3D co-culture assays showed that the directional migration abilities of the co-cultured SACC-83 cells and SCs were significantly increased. Under the stimulation of NT-3, the directional motor ability of SACC-83 cells and SCs was significantly improved, and the apoptosis of SACC-83 cells and SCs were obviously inhibited. In addition, blocking TrkC by its specific inhibitor AZD7451 could significantly inhibit these effects. Immunohistochemistry staining showed that the positive expression of NT-3 (88.5%) and TrkC (92.3%) was significantly correlated with the PNI in SACC specimens (P < 0.05). Additionally, the high expression of NT-3 was significantly associated with the poor prognosis of SACC patients (P < 0.05). The present study indicated that NT-3/TrkC axis contributed to the PNI progression and the poor prognosis of SACC via regulating the interaction between SACC cells and SCs. Interruption of the interaction between SACC cells and SCs by blocking the NT-3/TrkC axis might be an effective strategy for anti-PNI therapy in SACC.

19.
Exp Ther Med ; 18(2): 1039-1046, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31316600

RESUMO

The present study evaluated the effects of calcitonin gene-related peptide (CGRP) on bone marrow mesenchymal stem cells (BMMSCs) in vitro and in a rat model of mandibular distraction osteogenesis (MDO). Rat BMMSCs were isolated then treated with CGRP or CGRP antagonist (CGRP8-37). The proliferation and migration ability of BMMSCs was determined using 5-bromo-2'-deoxyuridine and Transwell assays, respectively. Osteogenic-related gene expression was analyzed with reverse transcription-quantitative polymerase chain reaction. For the in vivo analysis, thirty MDO rats were randomly assigned to control, CGRP or CGRP8-37 groups. To evaluate the mobilization of BMMSCs, nestin and stromal cell-derived factor 1 (SDF-1) were detected by immunohistochemistry and ELISA. Rats were sacrificed following 14 days and new bone formation was assessed by histological and micro-computed tomography analysis. In the in vitro results, the CGRP group demonstrated significantly higher migration and proliferation, as well as enhanced alkaline phosphatase and runt-related transcription factor 2 expression compared with the control. In the in vivo experiments, bone mineral density of the newly formed bone in the CGRP group was significantly higher than controls. The nestin and SDF-1 expression in the CGRP group was also significantly upregulated. In conclusion, the present study demonstrated that CGRP administration increased new bone formation, possibly via enhancing BMMSC migration and differentiation in MDO rats.

20.
J Pharm Biomed Anal ; 171: 158-163, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-30999226

RESUMO

In this work, a method for the rapid and reliable analysis of ginkgo flavonoids was developed by using paper spray mass spectrometry (PS-MS) assisted by microwave-assisted hydrolysis (MAH). Kaempferol-D4, quercetin-D5, and isorhamnetin-D3 were used as internal standards (IS). Samples analyses were completed by simply treating with extraction, MAH, dilution, and quantitative analysis by PS-MS. Because of the absence of HPLC separation, the PS-MS analysis time was less than 20 s. The linear ranges of kaempferol, quercetin and isorhamnetin were 1.7-86, 1.7-86, and 1.3-68 mg/L, respectively, with linear coefficients (R2) of 0.9993-0.9998. The detection limits (LODs) were 0.1, 0.1, and 0.2 mg/L. Limits of quantifications (LOQs) were all less than 0.6 mg/L. Compared with HPLC results, there are no obvious differences. It can be concluded that the PS-MS method with isotope IS is accurate for the rapid analysis of ginkgo flavonoids in extracts and related products.


Assuntos
Flavonoides/análise , Ginkgo biloba/química , Espectrometria de Massas/métodos , Extratos Vegetais/química , Ensaios de Triagem em Larga Escala , Limite de Detecção , Padrões de Referência , Reprodutibilidade dos Testes , Fatores de Tempo
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