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1.
BMC Genomics ; 20(1): 283, 2019 Apr 11.
Artigo em Inglês | MEDLINE | ID: mdl-30975079

RESUMO

BACKGROUND: Members of the genus Bacillus are important plant growth-promoting rhizobacteria that serve as biocontrol agents. Bacillus paralicheniformis MDJK30 is a PGPR isolated from the peony rhizosphere and can suppress plant-pathogenic bacteria and fungi. To further uncover the genetic mechanism of the plant growth-promoting traits of MDJK30 and its closely related strains, we used comparative genomics to provide insights into the genetic diversity and evolutionary relationship between B. paralicheniformis and B. licheniformis. RESULTS: A comparative genomics analysis based on B. paralicheniformis MDJK30 and 55 other previously reported Bacillus strains was performed. The evolutionary position of MDJK30 and the evolutionary relationship between B. paralicheniformis and B. licheniformis were evaluated by studying the phylogeny of the core genomes, a population structure analysis and ANI results. Comparative genomic analysis revealed various features of B. paralicheniformis that contribute to its commensal lifestyle in the rhizosphere, including an opening pan genome, a diversity of transport and the metabolism of the carbohydrates and amino acids. There are notable differences in the numbers and locations of the insertion sequences, prophages, genomic islands and secondary metabolic synthase operons between B. paralicheniformis and B. licheniformis. In particular, we found most gene clusters of Fengycin, Bacitracin and Lantipeptide were only present in B. paralicheniformis and were obtained by horizontal gene transfer (HGT), and these clusters may be used as genetic markers for distinguishing B. paralicheniformis and B. licheniformis. CONCLUSIONS: This study reveals that MDJK30 and the other strains of lineage paralicheniformis present plant growth-promoting traits at the genetic level and can be developed and commercially formulated in agriculture as PGPR. Core genome phylogenies and population structure analysis has proven to be a powerful tool for differentiating B. paralicheniformis and B. licheniformis. Comparative genomic analyses illustrate the genetic differences between the paralicheniformis-licheniformis group with respect to rhizosphere adaptation.


Assuntos
Bacillus/genética , Evolução Molecular , Genômica , Adaptação Fisiológica/genética , Bacillus/metabolismo , Bacillus/fisiologia , Família Multigênica/genética , Filogenia
2.
J Cell Biochem ; 120(8): 14055-14064, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30963631

RESUMO

Androgens and androgen receptors are vital factors involved in prostate cancer progression, and androgen ablation therapies are commonly used to treat advanced prostate cancer. However, the acquisition of androgen ablation therapy resistance remains a challenge. Recently, androgen receptor splicing variants lacking the ligand-binding domain have been reported to play a critical role in the acquisition of androgen ablation therapy resistance. In the present study, we revealed that the messenger RNA expression and the protein levels of an androgen receptor variant 7 (AR-V7) were higher in prostate cancer tissue samples and in the AR-positive prostate cancer cell line, VCaP. In contrast, microRNA (miR)-30c-1-3p/miR-103a-2-5p expression was significantly downregulated in tumor tissues and cells. miR-30c-1-3p/miR-103a-2-5p overexpression could inhibit AR-V7 expression, suppress VCaP cell growth, and inhibit AR-V7 downstream factor expression by directly targeting the 3'-untranslated region of AR-V7. Under enzalutamide (Enza) treatment, the effects of AR-V7 overexpression were the opposite of those of miR-103a-2-5p/miR-30c-1-3p overexpression; more importantly, the effects of miR-103a-2-5p/miR-30c-1-3p overexpression could be significantly reversed by AR-V7 overexpression under Enza. In summary, we demonstrated a novel mechanism of the miR-30c-1-3p/miR-103a-2-5p/AR-V7 axis modulating the cell proliferation of AR-positive prostate cancer cells via AR downstream targets. The clinical application of miR-30c-1-3p/miR-103a-2-5p needs further in vivo validation.

3.
Genome Announc ; 5(35)2017 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-28860261

RESUMO

Bacillus velezensis GQJK49 is a plant growth-promoting rhizobacterium with antifungal activity, which was isolated from Lycium barbarum L. rhizosphere. Here, we report the complete genome sequence of B. velezensis GQJK49. Twelve gene clusters related to its biosynthesis of secondary metabolites, including antifungal and antibacterial antibiotics, were predicted.

4.
Genome Announc ; 5(25)2017 Jun 22.
Artigo em Inglês | MEDLINE | ID: mdl-28642380

RESUMO

Bacillus paralicheniformis MDJK30 was isolated from the rhizosphere of a peony. It could control the pathogen of peony root rot. Here, we report the complete genome sequence of B. paralicheniformis MDJK30. Eleven secondary metabolism gene clusters were predicted.

5.
Genome Announc ; 5(24)2017 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-28619794

RESUMO

Bacillus velezensis JTYP2 was isolated from the leaves of Echeveria laui in Qingzhou, China, and may control some of the fungal pathogens of the plant. Here, we present the complete genome sequence of B. velezensis JTYP2. Several gene clusters related to its biosynthesis of antimicrobial compounds were predicted.

6.
Genome Announc ; 5(22)2017 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-28572331

RESUMO

Bacillus subtilis GQJK2 is a plant growth-promoting rhizobacterium with antifungal activity which was isolated from Lycium barbarum L. rhizosphere. Here, we report the complete genome sequence of B. subtilis GQJK2. Ten gene clusters involved in the biosynthesis of antagonistic compounds were predicted.

7.
Carbohydr Polym ; 94(1): 394-9, 2013 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-23544554

RESUMO

Self-assembled amphiphilic N-(2,3-dihydroxypropyl)-chitosan-cholic acid (DHP-CS-CHO) micelle was prepared as a carrier for paclitaxel. DHP-CS-CHO was synthesized by grafted small molecules cholic acid and glycidol onto primary amine group of chitosan, respectively. The DHP-CS-CHO formed uniform micelles (size=212.4±3.1 nm) with a low critical micelle concentration (0.024 mg/ml) in PBS. Hydrophobic anticancer drug, paclitaxel (PTX), was easily encapsulated into chitosan derivative micelles by a dialysis method with loading efficiency up to 80%. The PTX loaded micelles released the drug in a sustained manner more than a week in PBS containing 0.1% (w/v) Tween 80 at 37°C. In vitro antitumor experiment demonstrated that PTX loaded chitosan derivative micelles could inhibit MCF-7 cell growth and induce its apoptosis. These results suggested that DHP-CS-CHO may be a promising carrier for the anticancer drug PTX.


Assuntos
Antineoplásicos Fitogênicos/química , Quitosana/análogos & derivados , Quitosana/química , Ácido Cólico/química , Portadores de Fármacos/química , Paclitaxel/química , Apoptose/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Quitosana/síntese química , Preparações de Ação Retardada/química , Humanos , Cinética , Células MCF-7 , Micelas , Tamanho da Partícula , Tensoativos
8.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 36(2): 174-7, 2011 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-21368430

RESUMO

To discuss the pathology, clinical manifestation, diagnosis, therapy and prognosis of mature teratoma in adrenal gland. We reported 2 cases of mature adrenal teratoma and summarized their characters. No local recurrence or metastasis was found in the close follow-up of the 2 patients after the radical resection. Teratomas are rare germ tumors with potential malignant character which usually occur in the testes and ovaries. Adrenal teratomas are extremely rare and the diagnosis relies on radiological examinations. Early diagnosis and early surgical resection are the key in the treatment. Whether the postoperative chemotherapy is needed depends on the pathological result. Close lifelong follow-up is recommended and the prognosis is unclear at present.


Assuntos
Neoplasias das Glândulas Suprarrenais/cirurgia , Teratoma/cirurgia , Neoplasias das Glândulas Suprarrenais/diagnóstico por imagem , Adulto , Pré-Escolar , Feminino , Humanos , Teratoma/diagnóstico por imagem , Tomografia Computadorizada por Raios X
9.
Acta Crystallogr Sect E Struct Rep Online ; 65(Pt 11): m1316, 2009 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-21578079

RESUMO

In the crystal structure of the title compound, [Co(H(2)O)(6)][Cu(C(12)H(11)N(2)O(5))](2)·6H(2)O, the Co(II) atom is located on an inversion center and coordinated by six water mol-ecules in a slightly distorted octa-hedral geometry. The Cu(II) atom is chelated by the Schiff base ligand in a distorted CuN(2)O(2) square-planar geometry. An extensive O-H⋯O hydrogen-bonding network is present in the crystal structure.

10.
Nan Fang Yi Ke Da Xue Xue Bao ; 28(12): 2272-3, 2008 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-19114377

RESUMO

OBJECTIVE: To assess the clinical value of modified transabdominal preperitoneal (TAPP) repair of groin hernias in adult patients. METHOD: From May 2006 to April 2008, modified TAPP repair for hernia was performed in 403 adult patients with groin hernia and 22 with femoral hernia. Indirect hernia sac was treated by high ligation of the hernia sac in similar fashion with the management in children, while direct or femoral hernia was treated by dissecting the hernia sac. An incision was made on the lateral umbilical ligament, and the mesh was stapled and covered completely by the peritoneum of the lateral umbilical ligament, followed by fixation of the mesh with stapling and absorbable sutures. RESULTS: All the operations were accomplished successfully with the operating time from 20 to 30 min for unilateral hemioplasty and the blood loss volume was 4-5 ml. Two patients developed scrotal edema and three showed scrotal seroma. No hernia recurrence was found in follow-up for 2-22 months. The patients complained of no intestinal obstruction symptoms including traction pain, abdominal pain, or nausea or urinary bladder stimulation symptoms. CONCLUSIONS: Modified TAPP repair of groin hernias requires simple operation and produces reliable effect, and is therefore of clinical values for wide application.


Assuntos
Hérnia Inguinal/cirurgia , Laparoscopia/métodos , Peritônio/cirurgia , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Telas Cirúrgicas , Procedimentos Cirúrgicos Operatórios/métodos , Resultado do Tratamento , Adulto Jovem
12.
Zhonghua Wei Chang Wai Ke Za Zhi ; 8(2): 135-6, 2005 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-16155824

RESUMO

OBJECTIVE: To evaluate the clinical significance of microlaparoscopic treatment for adhesive intestinal obstruction in children. METHODS: Adhesion -lysis operation was performed in 18 patients with adhesive intestinal obstruct ion by microlaparoscope from March 2001 to January 2004. The clinical data were analyzed retrospectively. RESULTS: Microlaparoscope assisted accretion-lysis operation was successfully performed in all patients. The operative time ranged from 15 min to 45 min (averaged 30 min), the aerofluxus time ranged from 12 hours to 28 hours (averaged 22 hours) after operation. All patients can take general activity at the first day and take in food at the second day after operation respectively. The hospital stay ranged from 3 to 5 days (averaged 4 days) without complications. All patients were followed-up from 3 months to 18 months (average d 6 months). All patients recovered well without occurrence of adhesive intestinal obstruction. CONCLUSIONS: Micro-laparoscope assisted adhesion-lysis operation is a simple,safe,and feasible approach for adhesive intestinal obstruction in children.


Assuntos
Obstrução Intestinal/cirurgia , Laparoscopia , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Recém-Nascido , Obstrução Intestinal/patologia , Masculino , Estudos Retrospectivos , Aderências Teciduais
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