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1.
J Hum Genet ; 64(8): 815-820, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31164702

RESUMO

Genghis Khan's lineage has attracted both academic and general interest because of its mystery and large influence. However, the truth behind the mystery is complicated and continues to confound the scientific study. In this study, we surveyed the molecular genealogy of Northwestern China's Lu clan who claim to be the descendants of the sixth son of Genghis Khan, Toghan. We also investigated living members of the Huo and Tuo clans, who, according to oral tradition, were close male relatives of Lu clan. Using network analysis, we found that the Y-chromosomal haplotypes of Lu clan mainly belong to haplogroup C2b1a1b1-F1756, widely prevalent in Altaic-speaking populations, and are closely related to the Tore clan from Kazakhstan, who claim to be the descendants of the first son of Genghis Khan, Jochi. The most recent common ancestor of the special haplotype cluster that includes the Lu clan and Tore clan lived about 1000 years ago (YA), while the Huo and Tuo clans do not share any Y lineages with the Lu clan. In addition to the reported lineages, such as C3*-Star Cluster, R1b-M343, and Q, our results indicate that haplogroup C2b1a1b1-F1756 might be another candidate of the true Y lineage of Genghis Khan.

2.
Int J Pediatr Otorhinolaryngol ; 120: 58-63, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30771554

RESUMO

OBJECTIVES: Asthma and allergic rhinitis (AR) frequently occur as comorbid diseases of the upper airways. Single-nucleotide polymorphisms (SNPs) in the FCRL3 and FCRL5 genes have recently been shown to be associated with various immune-related disorders. This study evaluated the association of FCRL3 and FCRL5 polymorphisms with asthma and allergic rhinitis (AR) in a Han Chinese population. METHODS: Seven single nucleotide polymorphisms (SNPs) of the FCRL3 and FCRL5 were genotyped in 300 asthmatic children, and 206 healthy unrelated individuals using PCR-restriction fragment length polymorphism (PCR-RFLP) assay. Genotyping was validated by direct sequencing. RESULTS: Our results showed that the frequencies of the rs6692977 CT genotype and T allele within FCRL5 were significantly higher in asthma with comorbid AR compared to healthy controls (Bonferroni-corrected p (Pc) = 3.75 × 10-6; Pc = 0.006, respectively), whereas these of the CC genotype and C allele were significantly lower (Pc = 4.15 × 10-5; Pc = 0.006, respectively). The frequencies of the rs7528684 A allele (Pc = 1.80 × 10-3) and the rs10489678 G allele (Pc = 0.04) within FCRL3 were higher in asthma with comorbid AR than in controls. However, no differences in the tested genetic polymorphisms were detected between asthma and healthy individuals. CONCLUSION: This study identified novel SNPs in FCRL3 and FCRL5 significantly associated with the risk for asthma with comorbid AR in the Chinese population. The genetic variants may play role in the development of the asthma phenotype in children with asthma.


Assuntos
Grupo com Ancestrais do Continente Asiático/genética , Asma/genética , Receptores Fc/genética , Receptores Imunológicos/genética , Rinite Alérgica/genética , Adolescente , Alelos , Criança , Pré-Escolar , Feminino , Predisposição Genética para Doença , Genótipo , Humanos , Lactente , Masculino , Reação em Cadeia da Polimerase/métodos , Polimorfismo de Fragmento de Restrição , Polimorfismo de Nucleotídeo Único , Fatores de Risco
3.
Arch. argent. pediatr ; 116(5): 316-321, oct. 2018. ilus, tab
Artigo em Espanhol | LILACS-Express | ID: biblio-973661

RESUMO

Objetivo. Identificar las ventajas posoperatorias de la amigdalectomía en conjunto con la faringoplastia en comparación con la amigdalectomía sola en niños con apnea obstructiva del sueño. Métodos. En un estudio prospectivo observacional, los pacientes que cumplían con los criterios del estudio fueron asignados aleatoriamente a dos grupos: el grupo de amigdalectomía y faringoplastia, y el grupo de solo amigdalectomía. En ambos grupos, también se practicó adenoidectomía a los pacientes con vegetaciones. Se compararon los procesos de cicatrización en la herida faríngea y las proporciones hemorrágicas. Además, se evaluó la función velofaríngea posoperatoria. Resultados. La faringoplastia junto con la amigdalectomía se practicó en 328 niños con apnea obstructiva del sueño debido a hipertrofia amigdalina, y la amigdalectomía sola, en 275 niños. Estas cohortes no mostraban diferencias demográficas significativas. Por otro lado, se encontró que la pérdida de sangre fue significativamente menor en el grupo que recibió amigdalectomía y faringoplastia (p < 0,01), y el proceso de cicatrización fue notablemente más breve. La función velofaríngea posoperatoria no resultó afectada. Conclusiones. En comparación con la amigdalectomía, la amigdalectomía junto con la faringoplastia redujeron la duración del proceso de cicatrización y la pérdida de sangre; no obstante, las complicaciones posoperatorias no aumentaron. En nuestra opinión, la combinación de faringoplastia y amigdalectomía reúne un gran potencial en el tratamiento de los niños con apnea obstructiva del sueño.


Purpose. The study aims to identify the postoperative advantages of tonsillectomy in conjunction with pharyngoplasty and tonsillectomy alone in children with obstructive sleep apneas. Methods. In a prospective observational study, patients who met the study criteria were randomly divided into two groups: tonsillectomy and pharyngoplasty group, and tonsillectomy alone group. In both groups, adenoidectomy was also performed in patients with adenoid hypertrophy. Differences in their healing processes in the pharyngeal wound and their hemorrhage proportions were compared. Furthermore, postoperative velopharyngeal function was also assessed. Results. Pharyngoplasty together with tonsillectomy was performed in 328 children with obstructive sleep apnea from tonsillar hypertrophy, and tonsillectomy was performed in 275 children. These cohorts did not reveal any significant demographic differences between groups. Furthermore, blood loss was found to be significantly decreased in the tonsillectomy and pharyngoplasty group (P <0.01), and the healing process was markedly shorter. Postoperative velopharyngeal function was not affected. Conclusions. Tonsillectomy in conjunction with pharyngoplasty reduced the duration of the healing process and blood loss, compared with tonsillectomy alone; however, postoperative complications did not increase. We consider pharyngoplasty in conjunction with tonsillectomy has great potential in the treatment of children with obstructive sleep apnea.

4.
Arch Argent Pediatr ; 116(5): 316-321, 2018 Oct 01.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-30204981

RESUMO

PURPOSE: The study aims to identify the postoperative advantages of tonsillectomy in conjunction with pharyngoplasty and tonsillectomy alone in children with obstructive sleep apneas. METHODS: In a prospective observational study, patients who met the study criteria were randomly divided into two groups: tonsillectomy and pharyngoplasty group, and tonsillectomy alone group. In both groups, adenoidectomy was also performed in patients with adenoid hypertrophy. Differences in their healing processes in the pharyngeal wound and their hemorrhage proportions were compared. Furthermore, postoperative velopharyngeal function was also assessed. RESULTS: Pharyngoplasty together with tonsillectomy was performed in 328 children with obstructive sleep apnea from tonsillar hypertrophy, and tonsillectomy was performed in 275 children. These cohorts did not reveal any significant demographic differences between groups. Furthermore, blood loss was found to be significantly decreased in the tonsillectomy and pharyngoplasty group (P <0.01), and the healing process was markedly shorter. Postoperative velopharyngeal function was not affected. CONCLUSIONS: Tonsillectomy in conjunction with pharyngoplasty reduced the duration of the healing process and blood loss, compared with tonsillectomy alone; however, postoperative complications did not increase. We consider pharyngoplasty in conjunction with tonsillectomy has great potential in the treatment of children with obstructive sleep apnea.

5.
Am J Phys Anthropol ; 164(3): 522-532, 2017 11.
Artigo em Inglês | MEDLINE | ID: mdl-28782792

RESUMO

OBJECTIVES: The Tibetan-Yi Corridor located on the eastern edge of Tibetan Plateau is suggested to be the key region for the origin and diversification of Tibeto-Burman speaking populations and the main route of the peopling of the Plateau. However, the genetic history of the populations in the Corridor is far from clear due to limited sampling in the northern part of the Corridor. MATERIALS AND METHODS: We collected blood samples from 10 Tibetan and 10 Han Chinese individuals from Gansu province and genotyped about 600,000 genome-wide single nucleotide polymorphisms (SNPs). RESULTS: Our data revealed that the populations in the Corridor are all admixed on a genetic cline of deriving ancestry from Tibetans on the Plateau and surrounding lowland East Asians. The Tibetan and Han Chinese groups in the north of the Plateau show significant evidence of low-level West Eurasian admixture that could be probably traced back to 600∼900 years ago. DISCUSSION: We conclude that there have been huge population migrations from surrounding lowland onto the Tibetan Plateau via the Tibetan-Yi Corridor since the initial formation of Tibetans probably in Neolithic Time, which leads to the current genetic structure of Tibeto-Burman speaking populations.


Assuntos
Grupo com Ancestrais do Continente Asiático/genética , Fluxo Gênico/genética , Deriva Genética , Antropologia Física , Feminino , Genética Populacional , Migração Humana , Humanos , Masculino , Polimorfismo de Nucleotídeo Único/genética , Tibet
6.
Medicine (Baltimore) ; 96(19): e6628, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28489737

RESUMO

This study was designed to investigate the clinical effect of montelukast sodium combined with inhaled corticosteroids in the treatment of children with obstructive sleep apnea syndrome (OSAS).One hundred ninety-five children were enrolled and divided into 3 groups: groups A, B, and C; the group A (oral use of montelukast sodium), group B (nasal spray of mometasone furoate), and group C (oral use of montelukast sodium + nasal spray of mometasone furoate). Telephone questionnaire surveys were carried out. Polysomnography monitoring was performed and lateral x-ray radiographs of the cervical spine were taken before treatment and at 12 weeks after treatment. The improvement of clinical symptoms after treatment and its effective rate were analyzed. The difference in clinical characteristics between groups C1 and C2 was analyzed.In the 3 groups, clinical symptoms improved at 12 weeks after treatment compared with before (P < .05 or P < .01). Apnea-hypopnea index value decreased (P < .05) and minimal SaO2 increased (P < .05), while adenoidal/nasopharyngeal ratio was reduced (P < .05). Compared with groups A and B, group C had a shortened response duration of snoring, apnea, and restless sleep (P < .05). Differences in the response duration of buccal respiration and hyperhidrosis were not statistically significant (P > .05). The total effective rate was higher in group C than in A and B (P < .05), while the differences in all indices between groups A and B were not statistically significant (P > .05). The difference in the grade of the size of the tonsil between groups C1 and C2 was statistically significant (P < .05).The total effective rate of the combined treatment was higher than that of the single use of any of the 2 drugs, which allowed the rapid relief of symptoms. Drug treatment may have a poor curative effect in the treatment of OSAS patients with ≥ grade 3 tonsil hypertrophy.

7.
Sci Rep ; 7: 41195, 2017 01 23.
Artigo em Inglês | MEDLINE | ID: mdl-28112227

RESUMO

The origin and diversification of Sino-Tibetan speaking populations have been long-standing hot debates. However, the limited genetic information of Tibetan populations keeps this topic far from clear. In the present study, we genotyped 15 forensic autosomal short tandem repeats (STRs) from 803 unrelated Tibetan individuals from Gansu Province (635 from Gannan and 168 from Tianzhu) in northwest China. We combined these data with published dataset to infer a detailed population affinities and genetic substructure of Sino-Tibetan populations. Our results revealed Tibetan populations in Gannan and Tianzhu are genetically very similar with Tibetans from other regions. The Tibetans in Tianzhu have received more genetic influence from surrounding lowland populations. The genetic structure of Sino-Tibetan populations was strongly correlated with linguistic affiliations. Although the among-population variances are relatively small, the genetic components for Tibetan, Lolo-Burmese, and Han Chinese were quite distinctive, especially for the Deng, Nu, and Derung of Lolo-Burmese. Han Chinese but not Tibetans are suggested to share substantial genetic component with southern natives, such as Tai-Kadai and Hmong-Mien speaking populations, and with other lowland East Asian populations, which implies there might be extensive gene flow between those lowland groups and Han Chinese after Han Chinese were separated from Tibetans. The dataset generated in present study is also valuable for forensic identification and paternity tests in China.


Assuntos
Grupo com Ancestrais do Continente Asiático/genética , China , Genética Populacional , Genótipo , Humanos , Tibet
8.
Sci Rep ; 6: 38656, 2016 12 07.
Artigo em Inglês | MEDLINE | ID: mdl-27924949

RESUMO

There is a long-going debate on the genetic origin of Chinese Muslim populations, such as Uygur, Dongxiang, and Hui. However, genetic information for those Muslim populations except Uygur is extremely limited. In this study, we investigated the genetic structure and ancestry of Chinese Muslims by analyzing 15 autosomal short tandem repeats in 652 individuals from Dongxiang, Hui, and Han Chinese populations in Gansu province. Both genetic distance and Bayesian-clustering methods showed significant genetic homogeneity between the two Muslim populations and East Asian populations, suggesting a common genetic ancestry. Our analysis found no evidence of substantial gene flow from Middle East or Europe into Dongxiang and Hui people during their Islamization. The dataset generated in present study are also valuable for forensic identification and paternity tests in China.


Assuntos
Grupos Étnicos/genética , Genética Populacional , Islamismo , China , Análise por Conglomerados , Marcadores Genéticos , Humanos , Repetições de Microssatélites
9.
Am J Transl Res ; 8(10): 4329-4337, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27830016

RESUMO

PURPOSE: This study aims to determine whether leukotriene D4 (LTD4) can promote T cell proliferation in adenoid tissues via activation of CysLT1 receptors in children with OSAS. METHODS: CD4+ and CD8+ T cell proliferation was assessed by flow cytometry in adenoid mononuclear cells (AdMCs) stimulated with LTD4 from children with OSAS. The activation of mitogen-activated protein kinase pathways and their effects on the proliferation of CD4+ and CD8+ T cells in AdMCs were observed by western blotting. RESULTS: LTD4 increased the proliferation rates of both phytohemagglutinin (PHA)-stimulated CD4+ T cells (15.5±8.4% in the PHA group vs. 24.8±6.3% in the PHA+LTD4 group; n=27; P<0.001) and CD8+ T cells (17.2±5.9% in the PHA group vs. 23.5±5.2% in the PHA+LTD4 group; n=27; P<0.05) in AdMCs. LTD4 (10-4 mmol) significantly increased the phosphorylation of extracellular signal-regulated kinase (ERK1/2) and p38, but not c-Jun N-terminal kinases (JNK). The ERK1/2 inhibitor PD98059 significantly inhibited the proliferation of CD4+ and CD8+ T cells in LTD4-stimulated AdMCs. CONCLUSION: LTD4 regulates the proliferation of CD4+ and CD8+ T cells in PHA-stimulated AdMCs via upregulation of the ERK1/2 pathway. This finding indicates that CysLT1 receptors play a regulatory role in the pathogenesis of OSAS in children.

10.
Guang Pu Xue Yu Guang Pu Fen Xi ; 36(12): 3853-6, 2016 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-30234955

RESUMO

A series of plasma characteristic spectral lines of Mg alloy were obtained under nanosecond laser shock produced by a pulsed Nd : YAG laser (1 064 nm, maximum energy 500 mJ), which was taken under standard atmospheric pressure and at room temperature. Results indicated that the evolutionary rates of spectral lines were different, and the laser energy was enough to ionization Mg alloy under this experimental condition by the spectral lines of MgⅠ, MgⅡ. The electron temperature of Mg plasma were calculated by the measured relative Emission-line intensity(MgⅠ383.2 nm, MgⅠ470.3 nm, MgⅠ518.4 nm). The experimental results showed that the secondary excitation Mg atoms could be got under this experimental condition. The electron temperature of Mg plasma decreased with the laser energy reduced while the laser energy was in the range of 200~500 mJ. When the laser energy was in the range of 350~500 mJ, the electron temperature changed rapidly. When the laser energy was in the range of 200~350 mJ, the electron temperature changed slowly and tended towards stability. It is found that when the laser energy was 300 mJ, the plasma temperature raised suddenly, which could not accord with the trend because of plasma shielding. When the laser energy was 300 mJ, the relative intensity of spectral lines was reduced which was lower than that of 350 and 250 mJ. And it is against the variation trend of the relative intensity of spectral lines increases with the increase of laser energy, which prove plasma shielding phenomenon had occurred and plasma with high power laser separate the coupling between laser and material. The plasma temperature was increased significantly, which is not consistent with the trend .When the plasma shielding phenomenon happened, laser energy was absorbed by the plasma, resulting in the rise of plasma temperature.

11.
Zhongguo Zhong Xi Yi Jie He Za Zhi ; 36(11): 1364-1368, 2016 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-30641632

RESUMO

Objective To observe the effect of Astragalus membranaceus (AM) on expression levels of helper T cell 17 (Th17) and its related factors in patients with allergic rhinitis (AR). Methods Peripheral blood mononuclear cells (PBMCs) were sampled from 31 AR patients (recruited in the AR group) and 22 healthy subjects (recruited in the control group). PBMCs were isolated and in-vitro inter- vened by high and low dose AM injection (2 000 and 500 µg/mL) respectively for 24 h. mRNA expression levels of related orphan receptor C (RORC) were detected by real time quantitative PCR (RT-qPCR). Ex- pression levels of IL-17A and IL-22 in the supernatant were measured by ELISA. Expression levels of Th17 cells were measured by flow cytometry. Results Expression levels of Th17, RORC mRNA, IL- 17A, and IL-22 were higher in the AR group than in the control group (P <0. 01). mRNA expression levels of RORC, Th17 and its cytokines were not changed statistically in the AR group and the control group after PBMCs were intervened by low dose AM (P>0. 05). After intervened by high dose AM,mRNA expres- sion levels of RORC decreased statistically in the AR group and the control group (P <0. 05 for the AR group, P <0. 01 for the control group). Meanwhile,the expression levels of Th-17 and its cytokines de- creased in the AR group and the control group with statistical difference (P <0. 01). Conclusions Ex- cessive activation of Th17 is one of key factors-for AR. AM could further inhibit inflammation of AR and control the inflammation state of AR possibly through inhibiting the differentiation of Th17 cells and promo- ting the release of its cytokines.


Assuntos
Astragalus propinquus , Medicamentos de Ervas Chinesas , Extratos Vegetais , Rinite Alérgica , Células Th17 , Astragalus propinquus/química , Citocinas , Medicamentos de Ervas Chinesas/farmacologia , Humanos , Interleucina-17 , Leucócitos Mononucleares , Extratos Vegetais/farmacologia , RNA Mensageiro/metabolismo , Rinite Alérgica/tratamento farmacológico , Rinite Alérgica/imunologia
12.
Inflammation ; 37(5): 1738-43, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-24803294

RESUMO

The mechanisms underlying the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP) remain largely unknown. CRSwNP has garnered considerable public health concern owing to its high incidence and unsatisfactory treatment outcomes. Herbal remedies are promising candidates for the treatment of CRSwNP. We examined the utility of andrographolide, a diterpenoid lactone extracted from the Chinese herb Andrographis paniculata, an anti-inflammatory agent for CRSwNP treatment by evaluating interleukin (IL)-6 and IL-17 production and monitoring T helper 17 (Th17) differentiation of peripheral blood mononuclear cells (PBMCs) isolated from 20 Chinese CRSwNP patients and 11 control subjects. All CRSwNP patients exhibited clinical features of CRSwNP. Andrographolide significantly inhibited IL-6 and IL-17 production, suppressed p-Stat3 expression, and inhibited Th17 differentiation of PBMCs in vitro. These findings suggested that andrographolide has useful anti-inflammatory properties and could be used for the treatment of CRSwNP.


Assuntos
Diterpenos/farmacologia , Interleucina-6/metabolismo , Leucócitos Mononucleares/metabolismo , Pólipos Nasais/metabolismo , Rinite/metabolismo , Fator de Transcrição STAT3/metabolismo , Sinusite/metabolismo , Adulto , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/uso terapêutico , Células Cultivadas , Doença Crônica , Diterpenos/uso terapêutico , Relação Dose-Resposta a Droga , Feminino , Humanos , Interleucina-6/antagonistas & inibidores , Leucócitos Mononucleares/efeitos dos fármacos , Masculino , Pessoa de Meia-Idade , Pólipos Nasais/tratamento farmacológico , Rinite/tratamento farmacológico , Fator de Transcrição STAT3/antagonistas & inibidores , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/fisiologia , Sinusite/tratamento farmacológico , Adulto Jovem
13.
Lab Invest ; 94(5): 528-35, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24514067

RESUMO

A predominant Th17 population is a marker of allergic rhinitis (AR). The aryl hydrocarbon receptor (AhR) exhibits strong immunomodulation potential via regulation of the differentiation of T lymphocytes and dendritic cells (DCs) after activation by its ligand, such as 2-(1'H-indole-3'-carbonyl)-thiazole-4-carboxylic acid methyl ester (ITE). The aim of this study was to analyze the effect of AhR on Th17 differentiation by investigating the action of ITE on DCs and CD4(+) T cells from patients with AR. In all, 26 AR patients and 12 healthy controls were included in this study. The expression of interleukin (IL)-1ß, IL-6, IL-10, and IL-17 in the culture supernatant and the presence of Th17 cells in CD4(+) T cells and DC-CD4(+) T-cell co-culture system were measured before and after treatment with ITE. We show that ITE significantly induced cell secretion of IL-10 and inhibited IL-1ß and IL-6 production in DCs, and promoted IL-10 production and suppressed IL-17 expression in CD4(+) T cells in vitro. It also suppressed the expansion of Th17 cells in vitro. Our work demonstrates that ITE acts on DCs and CD4(+) T cells to inhibit the Th17 response that suppresses AR; the AhR-DC-Th17 axis may be an important pathway in the treatment of AR. ITE, a nontoxic AhR ligand, attenuated the Th17 response; thus, it appears to be a promising therapeutic candidate for suppressing the inflammatory responses associated with AR.


Assuntos
Células Dendríticas/metabolismo , Indóis/uso terapêutico , Receptores de Hidrocarboneto Arílico/agonistas , Rinite Alérgica Perene/tratamento farmacológico , Células Th17/metabolismo , Tiazóis/uso terapêutico , Estudos de Casos e Controles , Diferenciação Celular/efeitos dos fármacos , Células Cultivadas , Técnicas de Cocultura , Células Dendríticas/efeitos dos fármacos , Células Dendríticas/patologia , Avaliação Pré-Clínica de Medicamentos , Feminino , Humanos , Indóis/farmacologia , Interleucinas/metabolismo , Masculino , Receptores de Hidrocarboneto Arílico/metabolismo , Rinite Alérgica , Rinite Alérgica Perene/imunologia , Rinite Alérgica Perene/patologia , Células Th17/efeitos dos fármacos , Células Th17/patologia , Tiazóis/farmacologia
14.
Inflammation ; 37(2): 387-95, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24092408

RESUMO

A predominant Th17 population is a marker of chronic rhinosinusitis with nasal polyps (CRSwNP) in Chinese patients. As a ligand-activated transcription factor, the aryl hydrocarbon receptor (AhR) plays a vital role in promoting or inhibiting specific Th cell development. However, its role in CRSwNP remains to be defined. The aim of the present study was to investigate whether AhR, which regulates Th17 cell differentiation, played a role in the pathogenesis of CRSwNP by evaluating AhR expression in nasal polyps and peripheral blood mononuclear cells (PBMCs) obtained from CRSwNP patients. Forty-eight patients (atopic, 24; non-atopic, 24) and 13 controls were studied. To explore the role of AhR in CRSwNP, we analyzed the expression of AhR, retinoid-related orphan receptor C (RORC), interleukin (IL)-17, and IL-10 and the differentiation of Th17 using mRNA or protein detection methods. Notably, the expression of AhR was reduced in CRSwNP, and the expression of AhR was lower in the atopic group than in the non-atopic group. However, there was a very low level of Th17 and its associated factors (RORC, IL-17) in the control group compared to the two CRSwNP groups. In particular, the polarization of Th17 cells in atopic CRSwNP patients was increased compared with non-atopic individuals. In addition, ITE intervention in PBMCs promoted AhR expression and attenuated Th17 responses, demonstrating that AhR was more likely to suppress Th17 cells differentiation in Chinese CRSwNP patients. This information is valuable for obtaining a clear understanding of the pathogenesis of CRSwNP. Moreover, patients with atopic CRSwNP may exhibit reduced expression of AhR, leading to aggravation of the disproportionate distribution of Th17 cells in polyp tissues and PBMCs, thereby suggesting that atopic CRSwNP has a distinct pathogenesis from that of non-atopic CRSwNP.


Assuntos
Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo , Diferenciação Celular , Pólipos Nasais/metabolismo , Receptores de Hidrocarboneto Arílico/metabolismo , Rinite/metabolismo , Sinusite/metabolismo , Células Th17/metabolismo , Adulto , Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética , Estudos de Casos e Controles , Células Cultivadas , China , Doença Crônica , Dermatite Atópica/genética , Dermatite Atópica/imunologia , Dermatite Atópica/metabolismo , Feminino , Humanos , Interleucina-10/genética , Interleucina-10/metabolismo , Interleucina-17/genética , Interleucina-17/metabolismo , Masculino , Pessoa de Meia-Idade , Pólipos Nasais/genética , Pólipos Nasais/imunologia , Membro 3 do Grupo F da Subfamília 1 de Receptores Nucleares/genética , Membro 3 do Grupo F da Subfamília 1 de Receptores Nucleares/metabolismo , RNA Mensageiro/metabolismo , Receptores de Hidrocarboneto Arílico/genética , Rinite/genética , Rinite/imunologia , Transdução de Sinais , Sinusite/genética , Sinusite/imunologia , Células Th17/imunologia , Adulto Jovem
15.
Inflamm Res ; 62(3): 283-9, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23178794

RESUMO

BACKGROUND: A predominant Th17 population and impaired Treg function is the marker of nasal polyposis (NP) in Chinese patients. TGF-ß1, a multifunction cytokine, is a vital factor involved in inducing or restricting specific Th cell development. However, its role in NP has still not been well understood. METHODS: In a double-blind trial, 30 subjects were randomized into 2 groups (15 steroid-treated NP, 15 untreated NP), and 15 normal subjects were allocated as control group. We analyzed the expression of TGF-ß1, p-Smad2, p-STAT3, Smad7, SOCS3, IL-10, IL-17A, Foxp3, and RORc in the NP tissue of Chinese patients using mRNA and protein detection methods. RESULTS: TGF-ß1, p-Smad2, IL-10, SOCS3, and Foxp3 expression was higher in steroid-treated NP patients than in untreated NP patients. Conversely, expression of p-STAT3, Smad7, IL-17A, and RORc was higher in untreated NP patients than in steroid-treated NP patients, demonstrating that TGF-ß1 was more likely to contribute to Treg commitment in Chinese NP patients after intranasal steroid treatment. CONCLUSIONS: TGF-ß1 may be a signature Treg cytokine, which is valuable for obtaining a clear understanding of the pathogenesis of NP. Moreover, intranasal steroid treatment attenuated the chronic inflammatory response in these patients by promoting Smad-dependent Treg functions and reducing STAT3-mediated Th17 reactions.


Assuntos
Pólipos Nasais/tratamento farmacológico , Pólipos Nasais/fisiopatologia , Esteroides/uso terapêutico , Linfócitos T Reguladores/patologia , Linfócitos T Reguladores/fisiologia , Administração Intranasal , Adulto , China , Método Duplo-Cego , Feminino , Humanos , Interleucina-10/metabolismo , Interleucina-17/metabolismo , Masculino , Pessoa de Meia-Idade , Pólipos Nasais/metabolismo , Fator de Transcrição STAT3/metabolismo , Proteína Smad2/metabolismo , Proteína Smad7/metabolismo , Esteroides/administração & dosagem , Proteína 3 Supressora da Sinalização de Citocinas , Proteínas Supressoras da Sinalização de Citocina/metabolismo , Células Th17/patologia , Células Th17/fisiologia
16.
Artigo em Inglês | MEDLINE | ID: mdl-22678103

RESUMO

Transforming growth factor-ß1 (TGF-ß1) plays a key role in the tissue remodeling processes involved in chronic rhinosinusitis (CRS), with the biological functions of secreted TGF-ß1 regulated by multiple proteins. Among these regulators, latency-associated peptide and latent TGF-ß-binding protein inhibit TGF-ß1 function, whereas different proteases and integrins activate it. Progress in understanding the factors responsible for the bioactivity and expression of TGF-ß1 has revealed that the dysregulation of TGF-ß1 activation and expression is closely associated with the chronic respiratory inflammatory diseases involved in CRS. This review of the regulation of TGF-ß1 activation and expression provides insight into the mechanism responsible for the different CRS subtypes, which will help further the investigation of novel therapy targets for the treatment of CRS.


Assuntos
Rinite , Transdução de Sinais/fisiologia , Sinusite , Fator de Crescimento Transformador beta1/fisiologia , Doença Crônica , Humanos , Rinite/metabolismo , Rinite/patologia , Rinite/fisiopatologia , Sinusite/metabolismo , Sinusite/patologia , Sinusite/fisiopatologia
17.
Guang Pu Xue Yu Guang Pu Fen Xi ; 31(9): 2542-5, 2011 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-22097867

RESUMO

It is quick and accurate to on-line monitor the sample condition of laser cleaning by means of laser-induced plasma spectrum in air. In the present article, the echelle grating spectrometer was used to detect the plasma spectral lines induced by pulsed laser interaction with copper coin samples with or without contamination. The spectrogram showed that there were clear Cu I spectrum lines and air atom spectrum lines of N I and O I. In order to eliminate the uncertainty of single measurement, the statistical regularity of N I and O I spectrum lines was analyzed. Their intensity distribution laws were consistent and their relative standard deviations were the same basically. So a single measurement spectrum could be used to monitor cleaning process. The spectra of copper samples with contamination consisted of many elements atomic spectral lines and continuous spectral lines. But there are Cu I spectral lines in the spectra of clean copper samples. As a result, the authors could detect the change of spectral lines to judge whether the laser cleaning samples were clean.

19.
Guang Pu Xue Yu Guang Pu Fen Xi ; 27(6): 1090-2, 2007 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-17763764

RESUMO

The research on the technology of infrared absorption spectrum and its application plays an important role in the development of technology of optical measurement. The analysis technology of blood infrared absorption spectrum is presented in the present paper. By comparison between and analyzing the infrared absorption spectra of the normal blood sample and the high cholesterol blood sample, the differences were obtained. The infrared absorption spectra of normal blood serum and high cholesterol blood serum were detected, and the differences between the spectra are presented. The analysis results indicate that the differences of the absorptivity, the absorption peak position and the absorptivity ratio between the normal blood sample and the high cholesterol blood sample can be taken as a criterion to check the normal and abnormal blood sample. This work, valuable for application, provides an important reference for the diagnosis of diseases.


Assuntos
Colesterol/sangue , Hipercolesterolemia/diagnóstico , Espectrofotometria Infravermelho/métodos , Análise Química do Sangue/métodos , Humanos , Hipercolesterolemia/sangue , Sensibilidade e Especificidade
20.
Di Yi Jun Yi Da Xue Xue Bao ; 25(12): 1524-8, 2005 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-16361154

RESUMO

OBJECTIVE: To investigate the relationship between COX-2 expression and the clinicopathological factors in colorectal carcinoma and assess the prognostic value of COX-2 detection. METHODS: Tissue microarray and immunohistochemistry by SABC method was employed for detecting COX-2 expression in 126 patients with advanced colorectal cancer, and the relationship of COX-2 expression with the clinicopathological features and prognosis of the patients was retrospectively analyzed. RESULTS: The patients were divided into two groups of low and high COX-2 groups according to the grade and extent of COX-2 expression. High COX-2 expression was detected in 32 (25.4%) cases, and low expression in 94 (74.6%) cases. No significant correlation was noted between COX-2 expression and the patients' age, sex, tumor size, tumor location, histological type, lymphatic-node metastasis and Dukes' classification, but high COX-2 expression was strongly correlated with tumor recurrence and especially with blood-borne metastasis (P<0.05). The survival rate without tumor recurrence for high- and low-COX-2 groups was assessed by the Kaplan-Meier method and compared by log-rank test, which revealed significant difference between the two groups (P=0.0067). Multivariate analysis for all patients suggested that among the 8 prognostic factors (age, sex, tumor size, tumor location, histological type, lymphatic-node metastasis, Dukes' stage, and COX-2 expression), Dukes' stage and COX-2 expression was the independent significant factor related to disease-free survival. CONCLUSION: The expression of COX-2 is strongly correlated with recurrence of colorectal cancer, especially with blood-borne metastasis. COX-2 is an independent factor for prognostic evaluation of the patients, and tissue microarray allows rapid, convenient, economic and accurate COX-2 detection for large-scale application.


Assuntos
Biomarcadores Tumorais/biossíntese , Neoplasias Colorretais/metabolismo , Ciclo-Oxigenase 2/biossíntese , Proteínas de Membrana/biossíntese , Análise Serial de Tecidos/métodos , Adenocarcinoma/metabolismo , Adenocarcinoma/secundário , Adulto , Idoso , Idoso de 80 Anos ou mais , Neoplasias Colorretais/patologia , Ciclo-Oxigenase 2/genética , Feminino , Humanos , Metástase Linfática , Masculino , Proteínas de Membrana/genética , Pessoa de Meia-Idade , Recidiva Local de Neoplasia , Estudos Retrospectivos
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