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1.
Transcription ; : 1-11, 2020 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-31944157

RESUMO

Enhancers are cis-acting elements with many sites bound by transcription factors and activate transcription over long distance. Histone modifications are critical for enhancer activity and utilized as hallmarks for the identification of putative enhancers. Monomethylation of histone H3 lysine 4 (H3K4me1) is the mark for enhancer priming; acetylation of histone H3 lysine 27 (H3K27ac) for active enhancers and trimethylation of histone H3 lysine 27 (H3K27me3) for silent enhancers. Recent studies from multiple groups have provided evidence that enhancer reprogramming, especially gain of enhancer activity, is closely related to tumorigenesis and cancer development. In this review, we will summarize the recent discoveries about enhancer regulation and the mechanisms of enhancer reprogramming in tumorigenesis, and discuss the potential application of enhancer manipulation in precision medicine.

2.
J Cell Biochem ; 2020 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-31930562

RESUMO

MicroRNA-125a (miR-125a) is related to the occurrence, development, and prognosis of various cancers according to relevant reports. However, its function role and mechanism in non-small cell lung cancer (NSCLC) is yet to be explored. Herein, we investigated the role and preliminary mechanism of miR-125a in NSCLC. First, miR-125a was noticeably downregulated in NSCLC tissues in contrast to adjacent normal tissues through the real-time quantitative polymerase chain reaction (RT-qPCR) assay. The inverted result was observed on the STAT3 and HAS1 expressions. Moreover, miR-125a was expressed at highest level in A549 among four human NSCLC cell lines. Second, functional studies indicated miR-125a restrained proliferation, invasion, migration, metastasis, and advocated apoptosis of NSCLC cells, but had no obvious effect on cell cycle. Next, results indicated that a target of miR-125a was STAT3 on the basis of prediction and confirmation by the dual-luciferase reporter assay. RT-qPCR and Western blot assays displayed that miR-125a overexpression conspicuously constrained STAT3 expression at messenger RNA and protein levels. Finally, the binding between HAS1 promoter region and STAT3 was predicted by PROMO database analysis and verified by chromatin immunoprecipitation assay, suggesting that STAT3 was bound with the HAS1 promoter regions. STAT3 overexpression exerted positive effects on HAS1 expression at protein and mRNA levels. Additionally, HAS1-related functional studies illustrated HAS1 pronouncedly suppressed the proliferative, invasive, and migratory potential of NSCLC cells in vitro. Collectively, our findings demonstrated that miR-125a prohibited the proliferation, invasion, and migration of NSCLC cells by HAS1 expression reduction as a result of inhibiting STAT3 expression in NSCLC. This study indicated that miR-125a might be of potential or value for NSCLC treatment.

3.
Ann Vasc Surg ; 2020 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-31911130

RESUMO

OBJECTIVE: To develop and verify a risk predictive model/scoring system for pulmonary embolism (PE) among hospitalized patients with deep venous thrombosis of the lower extremities (LDVT). METHODS: 776 patients with LDVT were enrolled in a case-control study between January 2016 and June 2017 from the Vascular Surgery Department of Shanxi Dayi Hospital, China. They were randomly divided into development (543 patients, 70%) and validation (233 patients, 30%) databases. Based on the results of pulmonary computed tomography arteriography (PCTA), patients were divided into two categories; those with PE were designated as the case group while those without comprised the controls. A logistic regression model and scoring system for PE in patients with LDVT was established in the development database and verified in the validation database. Scoring system (Shanxi Dayi Hospital score - SDH score) was tabulated as follows: right lower extremity or bilateral lower extremities, 1; surgery or immobilization, 1; malignant tumor, 1; history of VTE, 2; D-dimer>1000 ng/mL, 2; and unprovoked, 2. Calibration and discrimination of the model were assessed by Hosmer-Lemeshow good-of-fit test and the area under the receiver operating characteristic curve (AUC). Wells score, the Revised Geneva score and the SDH score for predictive value of PE by AUC in the validation database, were compared. RESULTS: 776 patients with LDVT were divided into 2 risk categories based on the scores from the risk model, as follows: PE-unlikely (score <3); and PE-likely (score ≥3). Sensitivity, specificity, and crude agreement of the SDH score in the development database were 76.39%, 55.89%, and 61.33%, respectively. In the validation database, the logistic regression model showed good calibration and discriminative power. Hosmer-Lemeshow good-of-fit test P value was > 0.05 and the AUC was 0.705 (95% CI: 0.634∼0.776, P<0.001). The SDH score also showed good discriminative power, and the AUC was 0.702 (95% CI: 0.631∼0.774, P<0.001). Sensitivity, specificity, and crude agreement of the SDH score in the validation database were 67.61%, 61.73%, and 63.52%, respectively. AUC for the Wells score and the Revised Geneva score was 0.611 (95% CI: 0.533∼0.688, P=0.007) and 0.585 (95% CI: 0.503∼0.666, P=0.040). Difference of the AUC was not statistically significant between the Wells score and the SDH score (0.611 vs 0.702, P=0.059), but was so between the Revised Geneva score and the SDH score (0.585 vs 0.702, P= 0.016). Sensitivity of the Wells score, Revised Geneva score and the SDH score (64.79%, 67.61% vs 67.61%) were not statistically significant. However, the specificity of the Wells score and Revised Geneva score was significantly lower than that of the SDH score (48.77%, 39.51% vs 61.73%). CONCLUSIONS: Our logistic regression model and the SDH score based on 7 risk factors as right lower extremity, bilateral lower extremities, unprovoked, surgery or immobilization, malignant tumor, history of VTE, and D-dimer>1000 ng/ml, showed good calibration and discriminative power for the assessment of PE risk in patients with LDVT. The SDH score is more specific for PE prediction in the Chinese population, compared with the Wells score and the Revised Geneva score.

4.
Nano Lett ; 2019 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-31821007

RESUMO

Phosphorene (few-layer black phosphorus) has been widely investigated for its unique optical and electronic properties. However, it is challenging to synthesize and process stable phosphorene as it degrades rapidly upon exposure to oxygen and moisture under ambient conditions, which has limited its use in practical applications. Herein, we propose an alkali-assisted stabilization process to produce high-quality phosphorene nanosheets. Our morphology measurements show that alkali-treated phosphorene remains stable for over 7 days in air. Electrical measurements on alkali-treated BP devices further proved its stable electrical property under ambient conditions. We further demonstrate superior light-assisted electrochemical water splitting performance using stable phosphorene. We attribute the stabilization effect to the chemical modification of the surface of phosphorene with P-OH bond formation. This study paves the avenue for the implementation of phosphorene devices in ambient conditions.

5.
Onco Targets Ther ; 12: 9987-9997, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31819495

RESUMO

Purpose: Dysregulation of miRNAs plays an important role in the malignancy of different tumors including chordoma. Expression of miR-149-3p was earlier reported to be downregulated in chordoma tissue. However, its biological role remains to be unrevealed in chordoma, especially in spinal chordoma. Methods: Expression of miR-149-3p and Smad3 was detected by RT-qPCR and Western blot. Chordoma malignancy was evaluated by cell proliferation, migration, invasion, and apoptosis using MTT assay, transwell assay, flow cytometry analyzing apoptosis rate, and Western blot-determined expression of Bcl-2, Bax, and cleaved caspase 3, respectively. The target binding between miR-149-3p and Smad3 was predicted by TargetScan Human website and confirmed by luciferase reporter assay and RNA immunoprecipitation. Xenograft tumors were generated, and expression of miR-149-3p and Smad3 was investigated in vivo. Results: miR-149-3p was downregulated in spinal chordoma tissues and cells, and its overexpression promoted chordoma cell apoptosis and inhibited proliferation, migration, and invasion in U-CH1 and MUG-Chor1 cells. Unexpectedly, Smad3 was a downstream target of miR-149-3p and negatively correlated with miR-149-3p expression in chordoma tissues. Besides, Smad3 was upregulated in chordoma tissues and its silencing had a similar effect as miR-149-3p overexpression in U-CH1 and MUG-Chor1 cells. Moreover, Smad3 upregulation could partially reverse the tumor-suppressive effect of miR-149-3p in chordoma cells. In vivo, the tumorigenesis of U-CH1 and MUG-Chor1 cells was impaired by upregulated miR-149-3p through decreasing Smad3 expression. Conclusion: miR-149-3p could serve as a tumor suppressor in spinal chordoma through targeting and downregulating Smad3.

6.
Aquat Toxicol ; 218: 105352, 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31790938

RESUMO

Cadmium (Cd) affects plants and animal health seriously. Ca2+ signals in plant cells are important for adaptive responses to environmental stresses. Here we showed that 50 µM Cd shock stimulated the Ca2+ signal via modifying the instantaneous Ca2+ flux from influx of 17 pmol·cm-2·s-1 to the efflux of 240 pmol·cm-2·s-1 at 100 µm from rhizoid tip. And the Ca2+ signal transferred to the vein and mesophyll cell. The Ca addition decreased the accumulation of Cd. The gene expression of glutamate receptor-like (GLR) proteins, which is activated by Glu and triggers Ca2+ flux, was increased significantly by 24 h Cd stress. Glu content was increased under Cd stress and exogenous Glu triggered the Ca2+ signal in duckweed, while Ca2+ addition caused no influence to Glu content. GABA, which is synthesized from Glu and acts as an inhibitory neurotransmitter, has been decreased with 24 h Cd treatment. GABA addition increased the abscission rate and Glu addition decreased the abscission rate during Cd stress, suggesting that the Glu/GABA ratio is important for responding to Cd. This research shows the sight of the Glu, Ca2+, GABA signaling networks during Cd stress.

7.
Water Sci Technol ; 80(6): 1185-1195, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31799962

RESUMO

In this study, a full-scale survey was conducted of a sludge landfill that had been sealed for 10 years to investigate sludge properties, leachate characteristics and microbial community structure. Vertical distribution of sludge and leachate pollutants in the landfill site showed that the sludge and soluble pollutants in the leachate were both distributed almost evenly even after long-term anaerobic digestion, and higher concentrations of soluble pollutants and richness of microbial community were observed at the middle layer. Compared to dewatered excess sludge generated from the activated sludge process before landfill, landfill sludge had a much lower organic content (28.1%), smaller particle size and worse dewaterability. Compared to municipal waste landfill, sludge landfill generated leachate with a lower concentration of organic substances, and comparable concentrations of nitrogenous and phosphorus pollutants. Bacterial community analysis by Illumina MiSeq sequencing showed that Proteobacteria, Firmicutes, Chloroflexi, Actinobacteria and Bacteroidetes were the major phyla, and some new genera (Methylocystaceae, Mariniphaga and Aminicenantes) were enriched in the sludge landfill. Archaeal community analysis showed that aceticlastic methanogenesis by Methanosaeta and Methanosarcina was the main pathway for methane production in the sludge landfill, in contrast to waste landfill with hydrogenotrophic methanogenesis as the main pathway.

8.
Artigo em Inglês | MEDLINE | ID: mdl-31853905

RESUMO

PURPOSE: For the relief of right ventricular outflow tract obstruction in operative treatment of tetralogy of Fallot and other complex congenital heart diseases, transannular monocusp patch operations are often necessary to prevent right ventricular pressure overload and reduce pulmonary regurgitation. However, long-term durability of a monocusp leaflet is unsatisfactory, its failure is believed to be related to mechanical stress, whose distribution is primarily affected by geometric configurations. Therefore, the influence of several geometrical parameters on stress distribution of leaflet is investigated. METHODS: Five parameters affecting leaflet configuration were established: angle between free edge of the leaflet and vessel wall, angle formed by the two end points of free edge, length of the free edge of the leaflet, height of the leaflet, and shape of elliptic conical surface constituting the leaflet surface. The first four parameters were fixed, and two factors were defined to describe the last parameter. Seven models with different values of these factors were analyzed using finite element method at the pressure of the pulmonary artery loaded on the leaflet. RESULTS: The peak stresses of all models occurred at end points of the free edge of the leaflet (tear high-risk regions). The middle of leaflet had the greatest stress gradient and produced tissue wrinkling; this area could be the risk region of calcification. Both factors were noted to influence the stress distribution, and one of the factors could also relieve the wrinkling. CONCLUSIONS: The leaflet of model (1.2_min) had the most even stress distribution and lowest peak principal stress, which was the optimal choice among all the models.

9.
Artigo em Inglês | MEDLINE | ID: mdl-31863724

RESUMO

PURPOSE: Removal of foreign bodies in the craniomaxillofacial region can be challenging. The purpose of the present study was to explore the feasibility of using augmented reality (AR) technology to remove craniomaxillofacial foreign bodies. PATIENTS AND METHODS: A prospective study of patients with granular metal foreign bodies retained in the craniomaxillofacial region from March 2017 to March 2019 was performed. AR technology and navigation technology were both used to localize the foreign bodies. The face was divided into upper and lower parts by the ala-tragus line. In groups A and B, navigation technology was used to locate the foreign bodies in the upper face and lower face, respectively. Similarly, AR technology was used in the upper face and lower face in groups C and D, respectively. The primary predictor variable was the technology type. The primary outcome variables were the positioning deviation and the time required for surface positioning. The paired t test and independent samples t test were used for statistical analysis. RESULTS: Five patients with 24 craniomaxillofacial foreign bodies were included in the present study. The positioning deviation with navigation technology (1.42 ± 0.49 mm) did not differ from that with AR technology (1.52 ± 0.58 mm; P = .116). The positioning deviation of groups A, B, C, and D was 1.01 ± 0.37, 1.73 ± 0.29, 1.02 ± 0.44, and 1.89 ± 0.36 mm, respectively. The differences for groups A and B were statistically significant (P < .01), as were the differences for groups C and D (P < .01). The time required to position the 2 technologies was significantly different (10.04 ± 2.88 seconds for navigation technology and 3.46 ± 0.83 seconds for AR technology; P < .01). CONCLUSIONS: AR technology positioning was similar to that of navigation technology; however, it does not require an invasive registration device and provides real-time dynamic image guidance. AR technology could be an alternative method for treating foreign bodies in the craniomaxillofacial region.

10.
J Cell Mol Med ; 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31691530

RESUMO

The antitumour effect of melittin (MEL) has recently attracted considerable attention. Nonetheless, information regarding the functional role of MEL in bladder cancer (BC) is currently limited. Herein, we investigated the effect of MEL on critical module genes identified in BC. In total, 2015 and 4679 differentially expressed genes (DEGs) associated with BC were identified from the GSE31189 set and The Cancer Genome Atlas database, respectively. GSE-identified DEGs were mapped and analysed using Gene Ontology and Kyoto Encyclopaedia of Genes and Genomes analyses to determine BC-involved crucial genes and signal pathways. Coupled with protein-protein interaction network and Molecular Complex Detection analyses, Modules 2 and 4 were highlighted in the progression of BC. In in-vitro experiments, MEL inhibited the proliferation, migration, and invasion of UM-UC-3 and 5637 cells. The expression of NRAS, PAK2, EGFR and PAK1 in Module 4-enriched in the MAPK signalling pathway-was significantly reduced after treatment with MEL at concentrations of 4 or 6 µg/mL. Finally, quantitative reverse transcription-polymerase chain reaction and Western blotting analyses revealed MEL inhibited the expression of genes at the mRNA (ERK1/2, ERK5, JNK and MEK5), protein (ERK5, MEK5, JNK and ERK1/2) and phosphorylation (p-ERK1/2, p-JNK, and p-38) levels. This novel evidence indicates MEL exerts effects on the ERK5-MAK pathway-a branch of MAPK signalling pathway. Collectively, these findings provide a theoretical basis for MEL application in BC treatment.

11.
Biol Trace Elem Res ; 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31696354

RESUMO

The measurement of circulating metal ion levels in total hip arthroplasty patients continues to be an area of clinical interest. National regulatory agencies have recommended measurement of circulating cobalt and chromium concentrations in metal-on-metal bearing symptomatic total hip arthroplasty patients. However, the clinical utility of serum titanium (Ti) measurements is less understood due to wide variations in reported values and methodology. Fine-scale instrumentation for detecting in situ Ti levels continues to improve and has transitioned from graphite furnace atomic absorption spectroscopy to inductively coupled plasma optical emission spectrometry or inductively coupled plasma mass spectrometry. Additionally, analytical interferences, variable sample types, and non-standardized sample collection methods complicate Ti measurement and underlie the wide variation in reported levels. Normal reference ranges and pathologic ranges for Ti levels remain to be established quantitatively. However, before these ranges can be recognized and implemented, methodological standardization is necessary. This paper aims to provide background and recommendations regarding the complexities of measurement and interpretation of circulating Ti levels in total hip arthroplasty patients.

12.
Artigo em Inglês | MEDLINE | ID: mdl-31697230

RESUMO

A novel bacterium, designated TRM 44457T, belonging to the genus Streptomyces, was isolated from soil sampled in cotton fields in Xinjiang, PR China. Comparative 16S rRNA gene sequence analysis indicated that strain TRM 44457T was phylogenetically most closely related to Streptomyces laurentii LMG 19959T (99.38 % sequence similarity); however, strain TRM 44457T had a relatively low DNA-DNA relatedness value with S. laurentii LMG 19959T as determined by calculating the average nucleotide identity value (84.42 %). Strain TRM 44457T possessed ll-diaminopimelic acid as the diagnostic cell-wall diamino acid, MK-9 (H6) and MK-9 (H10) as the major menaquinone. The polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphotidylinositol, phosphatidylinositol mannosides and an unidentified phospholipid. The major fatty acids were anteiso-C15:0, iso-C16:0, anteiso-C17:0, iso-C15:0, C16:0, iso-C17:0, cyclo-C17:0 and anteiso-C17:1ω9c. The genomic DNA G+C content was 72.6 mol%. Based on the evidence from this polyphasic study, strain TRM 44457T represents a novel species of the Streptomyces, for which the name Streptomyces roseicoloratus is proposed. The type strain is TRM 44457T (=KCTC 39904T=CCTCC AA 2016040T).

13.
Onco Targets Ther ; 12: 8751-8763, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31749621

RESUMO

Background: The anticancer effects of cordyceps on various tumors have been reported. However, little is known about the role of selenium (Se)-enriched Cordyceps militaris in non-small cell lung cancer (NSCLC). In this study, the effects of Se-enriched Cordyceps militaris on cell proliferation, cell apoptosis and cell cycle in NSCLC cell line NCI-H292 and A549 were investigated. Methods: CCK-8 assay was used to determine the appropriate concentrations of Se-enriched Cordyceps militaris in NSCLC (namely NCI-H292 and A549) cells. Colony formation assay, flow cytometric and Hoechst 33342 staining assays, and flow cytometric analysis were separately employed to assess the effect of increased Se-enriched Cordyceps militaris on NSCLC cell viability, cell apoptosis and cell-cycle distribution. Finally, the qPCR and Western blot assays were, respectively, applied to evaluate the effects of Se-enriched Cordyceps militaris on the expression of pro-apoptotic member BAX and the anti-apoptotic member BCL-2, as well as of G2/M cell cycle regulatory proteins CDK1 and cyclin B1. Results: The concentration of Se-enriched Cordyceps militaris was 0, 4, 8, 12 mg/mL for NCI-H292 cells, and 0, 12.5, 25, 50 mg/mL for A549 cells. NSCLC cells treated with increased Se-enriched Cordyceps militaris showed the inhibited cell viability. Se-enriched Cordyceps militaris induced NSCLC cell apoptosis in concentration-dependent manner. Consistently, Se-enriched Cordyceps militaris diminished the ratio of anti-apoptotic member BCL-2 and pro-apoptotic member BAX at mRNA and protein levels in NSCLC cells. The percentage in G2/M phase was increased in NSCLC cells treated with increased Se-enriched Cordyceps militaris. Downregulation of G2/M cell cycle regulatory proteins CDK1 and cyclin B1 at mRNA and protein levels in NSCLC cells further confirmed the effects of Se-enriched Cordyceps militaris on cell cycle. Conclusion: This study demonstrated the inhibitory role of Se-enriched Cordyceps militaris in cell proliferation and its facilitating role in cell apoptosis and cell cycle in NSCLC cells, suggesting an alternative therapeutic strategy for NSCLC treatment.

14.
J Breast Cancer ; 22(3): 375-386, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31598338

RESUMO

Purpose: Although the effect of lysosome-associated protein transmembrane 4 beta (LAPTM4B) on the proliferation, migration, and invasion of breast cancer (BC) cells has already been studied, its specific role in BC progression is still elusive. Here, we evaluated the effect of different levels of LAPTM4B expression on the proliferation, invasion, adhesion, and tumor formation abilities of BC cells in vitro, as well as on breast tumor progression in vivo. Methods: We investigated the influence of LAPTM4B expression on MCF-7 cell proliferation, invasion, adhesion, and tube formation abilities in vitro through its overexpression or knockdown and on breast tumor progression in vivo. Results: Cell growth curves and colony formation assays showed that LAPTM4B promoted the proliferation of breast tumor cells. Cell cycle analysis results revealed that LAPTM4B promoted the entry of cells from the G1 into the S phase. Transwell invasion and cell extracellular matrix adhesion assays showed that LAPTM4B overexpression increased the invasion and adhesion capabilities of MCF-7 cells. More branches were observed in MCF-7 cells overexpressing LAPTM4B under an electron microscope. In comparison with LAPTM4B overexpression, LAPTM4B knockdown decreased the expression of vascular endothelial growth factor-A and significantly inhibited the vasculogenic tube formation ability of tumors. These results were also verified with western blot analysis. Conclusion: LAPTM4B promoted the proliferation of MCF-7 cells through the downregulation of p21 (WAF1/CIP1) and caspase-3, and induced cell invasion, adhesion, and angiogenesis through the upregulation of hypoxia-inducible factor 1 alpha, matrix metalloproteinase 2 (MMP2), and MMP9 expression. This specific role deems LAPTM4B as a potential therapeutic target for BC treatment.

15.
Bone Joint J ; 101-B(10): 1238-1247, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31564153

RESUMO

AIMS: Options for the treatment of intra-articular ligament injuries are limited, and insufficient ligament reconstruction can cause painful joint instability, loss of function, and progressive development of degenerative arthritis. This study aimed to assess the capability of a biologically enhanced matrix material for ligament reconstruction to withstand tensile forces within the joint and enhance ligament regeneration needed to regain joint function. MATERIALS AND METHODS: A total of 18 New Zealand rabbits underwent bilateral anterior cruciate ligament reconstruction by autograft, FiberTape, or FiberTape-augmented autograft. Primary outcomes were biomechanical assessment (n = 17), microCT (µCT) assessment (n = 12), histological evaluation (n = 12), and quantitative polymerase chain reaction (qPCR) analysis (n = 6). RESULTS: At eight weeks, FiberTape alone or FiberTape-augmented autograft demonstrated increased biomechanical stability compared with autograft regarding ultimate load to failure (p = 0.035), elongation (p = 0.006), and energy absorption (p = 0.022). FiberTape-grafted samples also demonstrated increased bone mineral density in the bone tunnel (p = 0.039). Histological evaluation showed integration of all grafts in the bone tunnels by new bone formation, and limited signs of inflammation overall. A lack of prolonged inflammation in all samples was confirmed by quantification of inflammation biomarkers. However, no regeneration of ligament-like tissue was observed along the suture tape materials. Except for one autograft failure, no adverse events were detected. CONCLUSION: Our results indicate that FiberTape increases the biomechanical performance of intra-articular ligament reconstructions in a verified rabbit model at eight weeks. Within this period, FiberTape did not adversely affect bone tunnel healing or invoke a prolonged elevation in inflammation. Cite this article: Bone Joint J 2019;101-B:1238-1247.


Assuntos
Lesões do Ligamento Cruzado Anterior/cirurgia , Reconstrução do Ligamento Cruzado Anterior/métodos , Polietilenos/química , Tendões/transplante , Resistência à Tração/fisiologia , Análise de Variância , Animais , Fenômenos Biomecânicos , Modelos Animais de Doenças , Feminino , Humanos , Peso Molecular , Coelhos , Sensibilidade e Especificidade , Estatísticas não Paramétricas , Técnicas de Sutura , Suturas , Transplante Autólogo
16.
Ann Anat ; : 151413, 2019 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-31655125

RESUMO

BACKGROUND: To conduct an anatomical study of the fibular collateral ligament (FCL), popliteus tendon (PT), biceps femoris tendon (BT) and popliteofibular ligament (PFL) of the knee joint posterolateral complex (PLC) at the femoral and fibular tendon-bone junctions based on the Chinese Visible Human (CVH) and American Visual Human Project (VHP) datasets and to determine their morphology, contact area, center points and mutual distances with the aim of providing assistance for surgical tunneling scheme. METHODS: Ten knee joint datasets were selected for segmentation and three-dimensional digital reconstruction. Histological sections images were used to establish criteria for the segmentation. The PLC tendon-junctions were observed and studied. RESULTS: The FCL and PT had constant attachment to the femur, and the FCL, BT and PFL had constant attachment to the fibula. The tendon-bone junctions of each PLC structure did not have a uniform morphology or the same contact area, but the location of the central point of the tendon-bone junction was similar and regularly attached. All measurements were smaller in the CVH dataset than VHP dataset. At the femoral tendon-bone junction, the average distance between the center points of the FCL and PT was 8.84 ±â€¯1.62 mm (7.73 ±â€¯1.44 mm in the CVH datasets and 9.50 ±â€¯1.38 mm in the VHP datasets). CONCLUSIONS: The authors propose a surgical tunneling scheme for femoral single-tunnel reconstruction in Chinese PLC reconstruction patients. The research data provide a theoretical basis and guidance for clinicians who need to design and select PLC surgical tunneling schemes.

17.
Nat Genet ; 51(11): 1580-1587, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31659325

RESUMO

Aortic calcification is an important independent predictor of future cardiovascular events. We performed a genome-wide association meta-analysis to determine SNPs associated with the extent of abdominal aortic calcification (n = 9,417) or descending thoracic aortic calcification (n = 8,422). Two genetic loci, HDAC9 and RAP1GAP, were associated with abdominal aortic calcification at a genome-wide level (P < 5.0 × 10-8). No SNPs were associated with thoracic aortic calcification at the genome-wide threshold. Increased expression of HDAC9 in human aortic smooth muscle cells promoted calcification and reduced contractility, while inhibition of HDAC9 in human aortic smooth muscle cells inhibited calcification and enhanced cell contractility. In matrix Gla protein-deficient mice, a model of human vascular calcification, mice lacking HDAC9 had a 40% reduction in aortic calcification and improved survival. This translational genomic study identifies the first genetic risk locus associated with calcification of the abdominal aorta and describes a previously unknown role for HDAC9 in the development of vascular calcification.

18.
ANZ J Surg ; 89(11): 1527, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31480093

RESUMO

Retraction: Feng Q, Liu J, Yao J. Common bile duct stones with situs inversus totalis. ANZ J Surg. https://doi.org/10.1111/ans.15387. The above article from the Australian and New Zealand Journal of Surgery, published online on 3 September 2019 in Wiley Online Library (wileyonlinelibrary.com), has been withdrawn by agreement between the journal's editor-in-chief, Julian A. Smith, and John Wiley & Sons, Inc. This action has been agreed due to an error at the publishers which caused a duplicate of the article to be published. The correct version of the article is: Feng Q, Yao J. Hepatobiliary and Pancreatic: common bile duct stones with situs inversus totalis. J Gastroenterol Hepatol. https://doi.org/10.1111/jgh.14833.

19.
Sensors (Basel) ; 19(18)2019 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-31527481

RESUMO

According to the existing mainstream automatic parking system (APS), a parking path is first planned based on the parking slot detected by the sensors. Subsequently, the path tracking module guides the vehicle to track the planned parking path. However, since the vehicle is non-linear dynamic, path tracking error inevitably occurs, leading to inclination and deviation of the parking. Accordingly, in this paper, a reinforcement learning-based end-to-end parking algorithm is proposed to achieve automatic parking. The vehicle can continuously learn and accumulate experience from numerous parking attempts and then learn the command of the optimal steering wheel angle at different parking slots. Based on this end-to-end parking, errors caused by path tracking can be avoided. Moreover, to ensure that the parking slot can be obtained continuously in the process of learning, a parking slot tracking algorithm is proposed based on the combination of vision and vehicle chassis information. Furthermore, given that the learning network output is hard to converge, and it is easy to fall into local optimum during the parking process, several reinforcement learning training methods in terms of parking conditions are developed. Lastly, by the real vehicle test, it is proved that using the proposed method can achieve a better parking attitude than using the path planning and path tracking-based method.

20.
Brain Sci ; 9(9)2019 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-31540407

RESUMO

Mental imagery is used extensively in the sporting domain. It is used for performance-enhancement purposes, arousal regulation, affective and cognitive modification, and rehabilitation purposes. The purpose of this experiment was to evaluate whether acute exercise and mental imagery of acute exercise have similar effects on cognitive performance, specifically memory function. A within-subject randomized controlled experiment was employed. Participants (N = 24; Mage = 21.5 years) completed two exercise-related visits (i.e., actual exercise and mental imagery of exercise), in a counterbalanced order. The acute-exercise session involved 10 min of intermittent sprints. The mental-imagery session involved a time-matched period of mental imagery. After each manipulation (i.e., acute exercise or mental imagery of acute exercise), memory was evaluated from a paired-associative learning task and a comprehensive evaluation of memory, involving spatial-temporal integration (i.e., what, where, and when aspects of memory). Bayesian analyses were computed to evaluate the effects of actual exercise and mental imagery of exercise on memory function. For the paired-associative learning task, there was moderate evidence in favor of the null hypothesis for a main effect for condition (BF01 = 2.85) and time by condition interaction (BF01 = 3.30). Similarly, there was moderate evidence in favor of the null hypothesis for overall (what-where-when) memory integration (BF01 = 3.37), what-loop (BF01 = 2.34), where-loop (BF01 = 3.45), and when-loop (BF01 = 3.46). This experiment provides moderate evidence in support of the null hypothesis. That is, there was moderate evidence to support a non-differential effect of acute exercise and mental imagery of acute exercise on memory function.

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