Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 543
Filtrar
1.
Sci Prog ; 104(3_suppl): 368504211054256, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34851210

RESUMO

INTRODUCTION: Curriculum learning through the wisdom tree massive open online course platform not only gets rid of the limitations of specialty, school and region, eliminates the limitations of time and space in traditional teaching, but also effectively solves the problem of educational equity. OBJECTIVES: This paper proposes an intelligent algorithm combining decision tree, support vector machine, and simulated annealing to obtain the best classification accuracy and decision rules for university students' satisfaction with the wisdom tree massive open online course platform. METHODS: This study takes the university students in Fuzhou city information management department as the survey object, and adopts the electronic questionnaire survey method. A total of 1136 formal questionnaires were responded, and 1028 valid questionnaires were obtained after data cleaning and deleting invalid questionnaires (the effective rate was 90.49%). In this paper, the reliability and validity of the questionnaire were tested by IBM SPSS-20.0 software, and six explanatory variables including function, achievement, exercise, quality, richness, and interaction were obtained by principal component analysis. Then, the questionnaire data is converted to CSV (comma separated values) format for analysis. This paper proposes an intelligent algorithm combining decision tree, support vector machine, and simulated annealing to obtain the best classification accuracy and decision rules for university students' satisfaction with the wisdom tree massive open online course platform. In this paper, the proposed algorithm is compared with decision tree, random forest, k-nearest neighbor, and support vector machine to verify its performance. RESULTS: The experimental results show that training set classification accuracy of decision tree, random forest, k-nearest neighbor, only support vector machine and the proposed algorithm (simulated annealing + support vector machine) are 92.21%, 96.10%, 95.67%, 97.29%, and 99.58%, respectively. CONCLUSION: The proposed algorithm simulated annealing + support vector machine does increase the classification accuracy. At the same time, the 11 decision rules generated by simulated annealing + decision tree can provide useful information for decision makers.

2.
Circ Genom Precis Med ; : CIRCGEN121003460, 2021 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-34732054

RESUMO

BACKGROUND: Elevated cardiac troponin levels in blood are associated with increased risk of cardiovascular diseases and mortality. Cardiac troponin levels are heritable, but their genetic architecture remains elusive. METHODS: We conducted a transethnic genome-wide association analysis on high-sensitivity cTnT (cardiac troponin T; hs-cTnT) and high-sensitivity cTnI (cardiac troponin I; hs-cTnI) levels in 24 617 and 14 336 participants free of coronary heart disease and heart failure from 6 population-based cohorts, followed by a series of bioinformatic analyses to decipher the genetic architecture of hs-cTnT and hs-cTnI. RESULTS: We identified 4 genome-wide significant loci for hs-cTnT including a novel locus rs3737882 in PPFIA4 and 3 previously reported loci at NCOA2, TRAM1, and BCL2. One known locus at VCL was replicated for hs-cTnI. One copy of C allele for rs3737882 was associated with a 6% increase in hs-cTnT levels (minor allele frequency, 0.18; P=2.80×10-9). We observed pleiotropic loci located at BAG3 and ANO5. The proportions of variances explained by single-nucleotide polymorphisms were 10.15% and 7.74% for hs-cTnT and hs-cTnI, respectively. Single-nucleotide polymorphisms were colocalized with BCL2 expression in heart tissues and hs-cTnT and with ANO5 expression in artery, heart tissues, and whole blood and both troponins. Mendelian randomization analyses showed that genetically increased hs-cTnT and hs-cTnI levels were associated with higher odds of atrial fibrillation (odds ratio, 1.38 [95% CI, 1.25-1.54] for hs-cTnT and 1.21 [95% CI, 1.06-1.37] for hs-cTnI). CONCLUSIONS: We identified a novel genetic locus associated with hs-cTnT in a multiethnic population and found that genetically regulated troponin levels were associated with atrial fibrillation.

3.
Biosens Bioelectron ; 196: 113733, 2021 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-34736102

RESUMO

Strong and stable electrochemical beacons are critical for the achievement of sensitive and reliable electroanalysis applications. In this work, the electrochemical oxidation performance of AuPt NPs was studied and firstly found to be largely enhanced under light illumination. Plasmonic AuPt NPs collected light energy after local surface plasmon resonance (LSPR) excitation and generated much more holes to participate in the electrochemical oxidation process of Pt0 in AuPt NPs. AuPt NPs with the electrochemical oxidation peak at around -0.7 V were utilized as detection probes for the fabrication of ratiometric electrochemical aptasensor, by introducing Co-MOF/Fe3O4/Ag nanosheets (NSs) with the electrochemical oxidation peak at 0.1 V as reference beacons. The aptamers of epithelial cell adhesion molecule (EpCAM) modified AuPt NPs were assembled with Co-MOF/Fe3O4/Ag NSs, which generated strong detection and reference signals at -0.7 V and 0.1 V, respectively. The high affinity between EpCAM and aptamers induced the separation of AuPt NPs from Co-MOF/Fe3O4/Ag NSs, resulting in the decrease of detection signal at -0.7 V and unchanged reference signal at 0.1 V. A ratiometric electrochemical aptasensor was achieved for the sensitive and reliable quantification of EpCAM in the range from 100 pg/mL to 100 ng/mL. The limit of detection (LOD) was calculated to be 13.8 pg/mL for EpCAM. Plasmon-driven electrochemical oxidation enhancement principle provides the possibility for the design and fabrication of more strong and anti-interference electroactive plasmonic metal-Pt composite nanostructures for the electroanalysis applications.

4.
Molecules ; 26(21)2021 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-34771118

RESUMO

In order to fully understand the morphological characteristics and pollution status of heavy metals in the dewatered sludge of Huainan Municipal sewage treatment plant, the physical and chemical properties were analyzed, and the content and occurrence forms of heavy metals (As, Cu, Zn, Pb, Cd, Cr, and Ni) in the sludge were studied using the geological accumulation method (Igeo), risk assessment coding method (RAC), and potential ecological risk index method to evaluate the ecological risk. The results showed that the municipal sludge in Huainan was rich in nutrients, with good prospects for agricultural utilization. There were differences in the morphological distributions of different heavy metals. The Igeo values for Ni, As, Cr, and Pb were below 0. The results of RAC indicated that the risk level of Cr in sludge was a low risk, and those of other heavy metals were moderate risks. The potential ecological risk of Cd had the highest potential ecological risk, and the other six metals were of low ecological risk. This conclusion can provide basic data and a theoretical reference for the comprehensive utilization of sludge in sewage treatment plants.

5.
Diabetes Care ; 2021 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-34789503

RESUMO

OBJECTIVE: LDL cholesterol (LDLc)-lowering drugs modestly increase body weight and type 2 diabetes risk, but the extent to which the diabetogenic effect of lowering LDLc is mediated through increased BMI is unknown. RESEARCH DESIGN AND METHODS: We conducted summary-level univariable and multivariable Mendelian randomization (MR) analyses in 921,908 participants to investigate the effect of lowering LDLc on type 2 diabetes risk and the proportion of this effect mediated through BMI. We used data from 92,532 participants from 14 observational studies to replicate findings in individual-level MR analyses. RESULTS: A 1-SD decrease in genetically predicted LDLc was associated with increased type 2 diabetes odds (odds ratio [OR] 1.12 [95% CI 1.01, 1.24]) and BMI (ß = 0.07 SD units [95% CI 0.02, 0.12]) in univariable MR analyses. The multivariable MR analysis showed evidence of an indirect effect of lowering LDLc on type 2 diabetes through BMI (OR 1.04 [95% CI 1.01, 1.08]) with a proportion mediated of 38% of the total effect (P = 0.03). Total and indirect effect estimates were similar across a number of sensitivity analyses. Individual-level MR analyses confirmed the indirect effect of lowering LDLc on type 2 diabetes through BMI with an estimated proportion mediated of 8% (P = 0.04). CONCLUSIONS: These findings suggest that the diabetogenic effect attributed to lowering LDLc is partially mediated through increased BMI. Our results could help advance understanding of adipose tissue and lipids in type 2 diabetes pathophysiology and inform strategies to reduce diabetes risk among individuals taking LDLc-lowering medications.

6.
Circulation ; 2021 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-34814699

RESUMO

Background: Plasma proteins are critical mediators of cardiovascular processes and are the targets of many drugs. Previous efforts to characterize the genetic architecture of the plasma proteome have been limited by a focus on individuals of European descent and leveraged genotyping arrays and imputation. Here we describe whole genome sequence analysis of the plasma proteome in individuals with greater African ancestry, increasing our power to identify novel genetic determinants. Methods: Proteomic profiling of 1,301 proteins was performed in 1852 Black adults from the Jackson Heart Study using aptamer-based proteomics (SomaScan®). Whole genome sequencing association analysis was ascertained for all variants with minor allele count ≥ 5. Results were validated using an alternative, antibody-based, proteomic platform (Olink®) as well as replicated in the Multi-Ethnic Study of Atherosclerosis and the HERITAGE Family Study. Results: We identify 569 genetic associations between 479 proteins and 438 unique genetic regions at a Bonferroni-adjusted significance level of 3.8 × 10-11. These associations include 114 novel locus-protein relationships and an additional 217 novel sentinel variant-protein relationships. Novel cardiovascular findings include new protein associations at the APOE gene locus including ZAP70 (sentinel single nucleotide polymorphism [SNP] rs7412-T, ß = 0.61±0.05, p-value = 3.27 × 10-30) and MMP-3 (ß = -0.60±0.05, p = 1.67 × 10-32), as well as a completely novel pleiotropic locus at the HPX gene, associated with nine proteins. Further, the associations suggest new mechanisms of genetically mediated cardiovascular disease linked to African ancestry; we identify a novel association between variants linked to APOL1 associated chronic kidney and heart disease and the protein CKAP2 (rs73885319-G, ß = 0.34±0.04, p = 1.34 × 10-17) as well as an association between ATTR amyloidosis and RBP4 levels in community dwelling individuals without heart failure. Conclusions: Taken together, these results provide evidence for the functional importance of variants in non-European populations, and suggest new biological mechanisms for ancestry-specific determinants of lipids, coagulation and myocardial function.

7.
Artigo em Inglês | MEDLINE | ID: mdl-34802868

RESUMO

INTRODUCTION: Cephalometry plays an important role in the diagnosis and treatment of orthodontics and orthognathic surgery. This study intends to develop an automatic landmark location system to make cephalometry more convenient. METHODS: In this study, 512 lateral cephalograms were collected, and 37 landmarks were included. The coordinates of all landmarks in the 512 films were obtained to establish a labeled dataset: 312 were used as a training set, 100 as a validation set, and 100 as a testing set. An automatic landmark location system based on the convolutional neural network was developed. This system consisted of a global detection module and a locally modified module. The lateral cephalogram was first fed into the global module to obtain an initial estimate of the landmark's position, which was then adjusted with the locally modified module to improve accuracy. Mean radial error (MRE) and success detection rate (SDR) within the range of 1-4 mm were used to evaluate the method. RESULTS: The MRE of our validation set was 1.127 ± 1.028 mm, and SDR of 1.0, 1.5, 2.0, 2.5, 3.0, and 4.0 mm were respectively 45.95%, 89.19%, 97.30%, 97.30%, and 97.30%. The MRE of our testing set was 1.038 ± 0.893 mm, and SDR of 1.0, 1.5, 2.0, 2.5, 3.0, and 4.0 mm were respectively 54.05%, 91.89%, 97.30%, 100%, 100%, and 100%. CONCLUSIONS: In this study, we proposed a new automatic landmark location system on the basis of the convolutional neural network. The system could detect 37 landmarks with high accuracy. All landmarks are commonly used in clinical practice and could meet the requirements of different cephalometric analysis methods.

8.
J Sports Sci ; : 1-7, 2021 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-34818995

RESUMO

There is no clear evidence for the best time of day for physical activity in benefitting health among individuals with type 2 diabetes. This study was aimed to examine whether there is an optimal time of day for physical activity to increase longevity. In this cohort study of 904 patients with type 2 diabetes, we fitted a Cox model with restricted cubic spline to examine the association of moderate-to-vigorous physical activity (MVPA) and time of day with all-cause mortality. Compositional data analysis was used to examine the association of time spent in sedentary behaviour (SB), light-intensity physical activity (LIPA), morning MVPA, and evening MVPA with all-cause mortality. MVPA was inversely associated with all-cause mortality in a curvilinear manner (P-value for nonlinearity<0.0001). The interaction between MVPA and time of day was not significant (P-value for interaction=0.11). Substituting evening MVPA with morning MVPA was not associated with appreciable changes in all-cause mortality. There is no evidence that the time of day moderates the association of MVPA with all-cause mortality. Patients with higher levels of MVPA had lower mortality regardless of the timing of MVPA. Patients with type 2 diabetes should engage in regular physical activity whenever possible.

9.
Biomed Eng Online ; 20(1): 119, 2021 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-34838009

RESUMO

PURPOSE: Long-leg-radiography (LLR) is commonly used for the measurement of lower limb alignment. However, limb rotations during radiography may interfere with the alignment measurement. This study examines the effect of limb rotation on the accuracy of measurements based on the mechanical and anatomical axes of the femur and tibia, with variations in knee flexion and coronal deformity. METHODS: Forty-five lower limbs of 30 patients were scanned with CT. Virtual LLRs simulating five rotational positions (neutral, ± 10[Formula: see text], and ± 20[Formula: see text] internal rotation) were generated from the CT images. Changes in the hip-knee-ankle angle (HKA) and the femorotibial angle (FTA) were measured on each image with respect to neutral values. These changes were related to knee flexion and coronal deformity under both weight- and non-weight-bearing conditions. RESULTS: The measurement errors of the HKA and FTA derived from limb rotation were up to 4.84 ± 0.66[Formula: see text] and 7.35 ± 0.88[Formula: see text], respectively, and were correlated with knee flexion (p < 0.001) and severe coronal deformity (p < 0.001). Compared with the non-weight-bearing position, the coronal deformity measured in the weight-bearing condition was 2.62[Formula: see text] greater, the correlation coefficients between the coronal deformity and the deviation ranges of HKA and FTA were also greater. CONCLUSIONS: Flexion and severe coronal deformity have a significant influence on the measurement error of lower limb alignment. Errors can be amplified in the weight-bearing condition compared with the non-weight-bearing condition. When using HKA and FTA to represent the mechanical axis and the anatomical axis on LLR, limb rotation impacts the anatomic axis more than the mechanical axis in patients with severe deformities. Considering LLR as the gold standard image modality, attention should be paid to the measurement of knee alignment. Especially for the possible errors derived from weight-bearing long-leg radiographs of patients with severe knee deformities.

10.
BMC Biol ; 19(1): 245, 2021 11 17.
Artigo em Inglês | MEDLINE | ID: mdl-34789250

RESUMO

BACKGROUND: The Mediator complex is an evolutionarily conserved multi-subunit protein complex that plays major roles in transcriptional activation and is essential for cell growth, proliferation, and differentiation. Recent studies revealed that some Mediator subunits formed nuclear condensates that may facilitate enhancer-promoter interactions and gene activation. The assembly, regulation, and functions of these nuclear condensates remain to be further understood. RESULTS: We found that Med15, a subunit in the tail module of the Mediator complex, formed nuclear condensates through a novel mechanism. Nuclear foci of Med15 were detected by both immunostaining of endogenous proteins and live cell imaging. Like Med1 foci and many other biomolecular condensates, Med15 foci were sensitive to 1, 6-Hexanediol and showed rapid recovery during fluorescence recovery after photobleaching. Interestingly, overexpressing DYRK3, a dual-specificity kinase that controls the phase transition of membraneless organelles, appeared to disrupt Med1 foci and Med15 foci. We identified two regions that are required to form Med15 nuclear condensates: the glutamine-rich intrinsically disordered region (IDR) and a short downstream hydrophobic motif. The optodroplet assay revealed that both the IDR and the C-terminal region of Med15 contributed to intracellular phase separation. CONCLUSIONS: We identified that the Mediator complex subunit Med15 formed nuclear condensates and characterized their features in living cells. Our work suggests that Med15 plays a role in the assembly of transcription coactivator condensates in the nucleus and identifies Med15 regions that contribute to phase separation.

11.
J Bone Joint Surg Am ; 2021 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-34780386

RESUMO

BACKGROUND: Following revision shoulder arthroplasty, postoperative antibiotics are selected before the results of intraoperative cultures become available. We determined infection-free survival, revision-free survival, complications, and patient-reported outcomes for patients selected to receive oral or intravenous (IV) antibiotics after revision arthroplasty. METHODS: This study included 92 patients who had revision shoulder arthroplasty. IV antibiotics were administered if the surgeon had a high index of suspicion for infection, and oral antibiotics were given if there was a low suspicion. Antibiotic therapy was modified based on intraoperative culture results. Patient-reported outcomes and adverse events were documented at a mean of 4.1 years. RESULTS: In selecting antibiotic therapy, surgeons correctly predicted the presence or absence of multiple positive cultures of specimens from the revision surgery in 72% of the 92 cases. Subsequent re-revision surgery was required in 17 (18%) of the patients; 8 of these 17 patients had ≥2 positive cultures at re-revision. Patients who initially received IV antibiotics and those who initially received oral antibiotics had similar revision-free (p = 0.202) and infection-free (p = 0.155) survivorship. Patients requiring a change from oral to IV antibiotics based on positive cultures had similar survivorship compared with those initially treated with IV antibiotics. The IV and oral antibiotic groups had similar postoperative Simple Shoulder Test (SST), American Shoulder and Elbow Surgeons (ASES), and satisfaction scores. Patients receiving IV antibiotics had a higher rate of antibiotic-related adverse events. CONCLUSIONS: Post-revision antibiotic therapy was associated with an infection-free survival rate of 91% at a mean of >4 years of follow-up. Infection-free survival, revision-free survival, and patient-reported outcomes were similar in high-risk patients placed on IV antibiotics and low-risk patients placed on oral antibiotics. Further study is needed to define the indications for IV or oral antibiotics after revision arthroplasty. LEVEL OF EVIDENCE: Therapeutic Level IV. See Instructions for Authors for a complete description of levels of evidence.

12.
Phys Rev E ; 104(4-1): 044410, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34781453

RESUMO

Sensor-to-actuator delay is inevitable in any complex control system, be it one for a free-flying insect or a mimicking insectlike robotic flyer. In this work, we analyze the effects of control delay (latency) on the hovering performance of a model insect flyer, as exemplified by the hummingbird hawkmoth Re∼3000, and determine how control coefficients or gains may be modified to ameliorate the adverse effects of latency. The analyses are based on a simplified or reduced dynamic model of the hovering flyer. The longitudinal dynamics of the hovering flyer comprises the coupled forward (backward) and vertical translations and pitch rotation of the flyer, with kinematical wing actions being governed by proportional-differential (PD) closed-loop control. Keeping to the same PD control coefficients as a stable reference zero-delay case, the flight system becomes overly responsive at a small control delay, eventually diverging when delay approaches around one wing cycle. Stable hovering may be regained for control delay of up to several wingbeats by suitably reducing or softening the PD control coefficients. The results of the analyses are validated by a series of time-based simulations using the simplified dynamic model and a high-fidelity three-dimensional computational fluid dynamics with fluid structure-body interaction model of the hovering flyer. The simulations also show that noncyclic asymptotic oscillations about the mean equilibrium hovering state are enhanced with larger control delay. The analyses and simulations have helped us to gain a better understanding of the effects of control latency in insect free flight, which may be relevant for the design of mimetic insect flyers.

13.
Membranes (Basel) ; 11(11)2021 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-34832133

RESUMO

This study in-situ modified a commercial nanofiltration membrane, NF90, through the concentration-polymerization-enhanced radical graft polarization method by applying two agents of 3-sulfopropyl methacrylate potassium salt (SPM) and 2-hydroxyethyl methacrylate (HEMA) with different dosages. Surface characterization revealed that the modified membranes became rougher and more hydrophilic compared with the pristine membrane. The modified membranes exhibited considerably enhanced separation performance with 5.8-19.6% higher NaCl rejection and 17.2-19.9% higher pharmaceuticals and personal care products (PPCPs) rejection than the pristine membrane. When treating the feedwater with high silica concentration, the modified membranes exhibited relatively less flux decline with high percentage of reversible fouling, especially the ones modified using a lower monomer concentration (0.01 M SPM and 0.01 M HEMA). Moreover, membrane modification enhanced the PPCP rejection (1.3-5.4%) after silica fouling by mitigating foulant deposition on the membrane surface. The fouling mechanism was confirmed to be intermediate blocking of membrane pores. Therefore, the in-situ modification technique with a low monomer concentration proved to be effective for mitigating silica fouling and improving PPCP rejection, which can be easily performed and cost-effective in practical application.

14.
Front Public Health ; 9: 733458, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34621722

RESUMO

Background: Employees who are physically present but work insufficiently because of illness are deemed as having presenteeism. In the health care setting, the issue has taken on greater importance because of the impairment of the physical and mental health of nurses and the nursing safety of the patients. According to the Job Demand-Resource Model, burnout may link emotional labor with presenteeism. Thus, this study analyzed the role of burnout as a mediating factor between the three types of emotional labor strategies and presenteeism among nurses in tertiary-level hospitals. Methods: A cross-sectional study of 1,038 nurses from six Chinese hospitals was conducted. The questionnaires, including the 14-item emotional labor strategies scale, 22-item Maslach Burnout Inventory scale, 6-item Stanford Presenteeism Scale, and items about demographic characteristics and work-related factors, were used to collect data. A multivariable linear regression was used to predict work-related factors and investigate the correlation of emotional labor, burnout, and presenteeism. The structural equation model was implemented to test the mediating effects of job burnout. Results: The results of the study showed that the average presenteeism score of the participants was 14.18 (4.33), which is higher than in Spanish, Portuguese, and Brazilian nurses. Presenteeism was explained by 22.8% of the variance in the final model in multivariable linear regression (P < 0.01). Presenteeism was found to be positively correlated with surface acting, emotionally expressed demands, deep acting, emotional exhaustion, depersonalization, and low personal accomplishment (P < 0.01). Notably, presenteeism was negatively correlated with deep acting (P < 0.01). In addition, burnout partially mediated the correlation between emotionally expressed demands, deep acting, and presenteeism with a mediatory effect of 24 and 63.31% of the total effect. Burnout completely mediated the association between surface acting and presenteeism, a mediating effect of 86.44% of the total effect. Conclusions: The results of this study suggested that different emotional labor strategies affect presenteeism, either directly or indirectly. Nursing managers should intervene to reduce presenteeism by improving the ability of the nurses to manage emotions, thereby alleviating burnout.


Assuntos
Esgotamento Profissional , Enfermeiras Administradoras , Esgotamento Profissional/epidemiologia , China/epidemiologia , Estudos Transversais , Emoções , Humanos , Presenteísmo , Centros de Atenção Terciária
15.
Artigo em Inglês | MEDLINE | ID: mdl-34601149

RESUMO

OBJECTIVES: Post-treatment recurrence remains a challenge for the global control of tuberculosis (TB). This study investigated longitudinal data on pulmonary TB recurrence rates and its risk factors among successfully treated smear-positive tuberculosis cases in China. METHODS: From 1 January 2009 to 31 December 2016, we evaluated 33,441 treatment-naïve patients diagnosed with sputum smear-positive, non-multidrug-resistant TB in Hangzhou, China. We included the data of 9,828 patients with TB who were successfully treated. RESULTS: A total of 4.9% were recurrent cases (479/9,828), identified within a median observation period lasting 1,565 days. Altogether, 51.1% (245/479) of the recurrences occurred within one year. The cumulative 2- and 5-year recurrence rates were 3.90% (95% confidence interval [CI], 3.3%-4.5%) and 5.4% (95% CI, 4.8%-6.0%), respectively. Prolonged treatment (over 7 months) was occurred in 64.7% (6,363/9,828), with median treatment duration of 242 (interquartile range, 195-348) days. Male sex (adjusted hazard ratio [aHR] [95% CI] = 1.61 [1.30-2.00], P<0.001), ≥ 60-years age (aHR [95% CI] = 2.03 [1.70-2.44], P<0.001), pulmonary cavity (aHR [95% CI] = 1.51 [1.25-1.82], P<0.001) and sputum positive at 2 months (aHR [95% CI] =1.39 [1.05-1.81], P=0.02) increased the risk of TB recurrence. Prolonged treatment was associated with reduced TB recurrence (aHR [95% CI] =0.73 [0.61-0.88], P=0.001). CONCLUSIONS: Recurrence remains a problem for successfully treated patients with sputum smear-positive pulmonary TB, especially those with independent risk factors. Further analysis of prolonged treatment is required.

16.
Front Pharmacol ; 12: 744483, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34712137

RESUMO

Nonalcoholic steatohepatitis (NASH) has become one of the serious causes of chronic liver diseases, characterized by hepatic steatosis, hepatocellular injury, inflammation and fibrosis, and lack of efficient therapeutic agents. Palmitoylethanolamide (PEA) is an endogenous bioactive lipid with various pharmacological activities, including anti-inflammatory, analgesic, and neuroprotective effects. However, the effect of PEA on nonalcoholic steatohepatitis is still unknown. Our study aims to explore the potential protective role of PEA on NASH and to reveal the underlying mechanism. In this study, the C57BL/6 mice were used to establish the NASH model through methionine- and choline-deficient (MCD) diet feeding. Here, we found that PEA treatment significantly improved liver function, alleviated hepatic pathological changes, and attenuated the lipid accumulation and hepatic fibrosis in NASH mice induced by MCD diet feeding. Mechanistically, the anti-steatosis effect of PEA may be due to the suppressed expression of ACC1 and CD36, elevated expression of PPAR-α, and the phosphorylation levels of AMPK. In addition, hepatic oxidative stress was greatly inhibited in MCD-fed mice treated with PEA via enhancing the expression and activities of antioxidant enzymes, including GSH-px and SOD. Moreover, PEA exerted a clear anti-inflammatory effect though ameliorating the expression of inflammatory mediators and suppressing the NLRP3 inflammasome pathway activation. Furthermore, the impaired autophagy in MCD-induced mice was reactivated with PEA treatment. Taken together, our research suggested that PEA protects against NASH through the inhibition of inflammation and restoration of autophagy. Thus, PEA may represent an efficient therapeutic agent to treat NASH.

17.
Front Pediatr ; 9: 741793, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34660493

RESUMO

We studied the demographic and clinical characteristic, risk factors, outcomes of full-term small-for-gestational-age (SGA) infants born to mothers with gestational diabetes mellitus (GDM) in China. A retrospective case-control study that included 1981 SGA infants was conducted; the demographic and clinical data between SGA infants born to mothers with and without GDM were compared. Of 383 SGA infants born to mothers with GDM, 221 (57.7%) were female, and the incidence of these infants was 1 in 155 live births. The risk of SGA siblings (RR, 1.88; 95% CI, [1.23-2.86]), low 1- and 5-min Apgar scores (RR,2.04 and 4.21; 95%CI [1.05-4.00] and [1.05-16.89], respectively), early thrombocytopenia (RR, 3.39; 95%CI, [1.33-8.64]), hypoglycemia(RR, 2.49; 95%CI, [1.55-3.98]), and hypoxic-ischemic encephalopathy (RR,5.61; 95%CI, [1.25-25.18]) were increased in SGA infants born to mothers with GDM compared to SGA infants born to mothers without GDM. SGA girls born to mothers with GDM had a significantly higher ratio of catch-up growth (CUG) (RR, 1.73; 95%CI, [1.18-2.54]) in the first year of life. These results show that genetic factors may be one of the etiologies of SGA infants born to mothers with GDM; and these infants have more adverse perinatal outcomes compared to SGA infants born to mothers without GDM. SGA girls born to mothers with GDM had accelerated CUG in the first year of life.

18.
PLoS One ; 16(10): e0259012, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34673812

RESUMO

OBJECTIVES: This study aims to investigate the potential factors associated with mental health outcomes among Chinese adults during the Coronavirus disease 2019 (COVID-19) epidemic. METHODS: This is an online cross-sectional survey conducted among Chinese adults in February 2020. Outcome measurements included the three-item UCLA Loneliness Scale (UCLA-3), two-item Patient Health Questionnaire (PHQ-2), two-item Generalized Anxiety Disorder Questionnaire (GAD-2), and two items from the Clinician-Administered Post-traumatic Stress Disorder (PTSD) Scale. COVID-19 related factors, physical health, lifestyle, and self-efficacy were also measured. Univariable and multivariable logistic regressions were performed. RESULTS: This study included 1456 participants (age: 33.8±10.5 years; female: 59.1%). The prevalence of depressive symptoms, anxiety symptoms, loneliness, and PTSD symptoms were 11.3%, 7.6%, 38.7%, and 33.9%, respectively. In multivariable analysis, loneliness was associated with being single, separated/divorced/widowed, low level of education, current location, medication, more somatic symptoms, lower self-efficacy, and going out frequently. Depression was associated with fear of infection, binge drinking, more somatic symptoms, lower self-efficacy, and longer screen time. Anxiety was associated with more somatic symptoms and lower self-efficacy. PTSD symptoms were associated with more somatic symptoms, lower self-efficacy, higher perceived risk of infection, fear of infection, and self-rated more negative influence due to the epidemic (p<0.05). CONCLUSIONS: Mental health problems during the COVID-19 epidemic were associated with various biopsychosocial and COVID-19 related factors. Psychological interventions should be aware of these influencing factors and prioritize support for those people at higher risk.


Assuntos
COVID-19 , Depressão , Solidão , SARS-CoV-2 , Transtornos de Estresse Pós-Traumáticos , Adulto , COVID-19/epidemiologia , COVID-19/psicologia , China/epidemiologia , Estudos Transversais , Depressão/epidemiologia , Depressão/etiologia , Depressão/psicologia , Feminino , Humanos , Masculino , Prevalência , Transtornos de Estresse Pós-Traumáticos/epidemiologia , Transtornos de Estresse Pós-Traumáticos/etiologia , Transtornos de Estresse Pós-Traumáticos/psicologia
19.
Microb Physiol ; : 1-12, 2021 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-34535588

RESUMO

Postnatal colonization and development of the gut microbiota is linked to health and growth. A comprehensive understanding of the postnatal compositional changes and development of the microbial community is helpful to understand the gut health and improve the survival rate of South China tiger cubs (Panthera tigris amoyensis). Fecal samples from three tiger cubs were collected on the day of birth in 2018 (June 17-21 [G0], July 18 [G1], July 31 [G2], and August 7 [G3]). The 16S rRNA genes of the fecal microflora were sequenced. Results showed that 38 phyla, 58 classes, 134 orders, 272 families, and 636 genera of bacteria from 3,059 operational taxonomic units were identified from 12 fecal samples. The diversity and abundance of species of group G0 were significantly higher (p < 0.05 or 0.01) than those of groups G2 and G3. The predominant phylum was Proteobacteria in groups G0 and G1 (38.85% and 48%, respectively) and Firmicutes in groups G2 and G3 (71.42% and 75.29%, respectively). At the phylum level, the abundance of Deinococcus-Thermus was significantly decreased in groups G1, G2, and G3 as compared to group G0 (p < 0.05), while that of Firmicutes was significantly increased in groups G2 and G3 (p < 0.05). At the genus level, the abundance of Faecalibacterium, Ralstonia, and unidentified Rickettsiales was significantly decreased in groups G1, G2, and G3 as compared with group G0 (p < 0.05), while that of Pseudomonas was significantly decreased in groups G2 and G3 (p < 0.05). The composition and structure of fecal microbiota of South China tiger cubs changed after birth.

20.
Oncogenesis ; 10(9): 62, 2021 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-34548478

RESUMO

Pancreatic carcinoma (PC) is one of the most common malignancies. Chimeric antigen receptor (CAR)-modified T cells has achieved remarkable efficacy in the treatment of hematological malignancies. However, lack of tumor-specific targets and the existence of inhibitory factors limit the function of CAR T cells when treating solid tumors. 4.1R has been reported to suppress the anti-tumor activity of T cell responses. In this study, we investigated the anti-tumor activity of 4.1R deletion in natural killer group 2D (NKG2D)-CAR T cells against PC. The CAR T cells were obtained by transfecting T cells with lentiviral vector carrying NKG2D-CAR, NC-NKG2D-CAR, or KD2-NKG2D-CAR. In vitro, NKG2D-CAR T cells showed higher cytotoxicity than Mock T cells. However, compared to NKG2D-CAR T cells, furtherly higher cytotoxicity against PC cells in a dose-dependent manner was found in KD2-NKG2D-CAR T cells. In addition, the proliferation rate and cytotoxic activity of KD2-NKG2D-CAR T cells were significantly higher than those of NKG2D-CAR T cells. Besides, the inhibitory receptors PD-1 and TIM-3 were expressed in lower level on KD2-NKG2D-CAR T cells. In vivo, KD2-NKG2D-CAR T cells suppressed tumor growth more effectively in a xenograft model compared to NKG2D-CAR T cells. Mechanistically, 4.1R regulated CAR T cell function via activating ERK signaling pathway. Therefore, the study provides a new idea to enhance the anti-tumor efficiency of CAR T therapy.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...