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1.
Artigo em Inglês | MEDLINE | ID: mdl-32543738

RESUMO

Smart self-protection is essential for addressing safety issues of energy-storage devices. However, conventional strategies based on sol-gel transition electrolytes often suffer from unstable self-recovery performance. Herein, smart separators based on thermal-gated poly(N-isopropylacrylamide) (PNIPAM) hydrogel electrolytes were developed for rechargeable zinc-ion batteries (ZIBs). Such PNIPAM-based separators not only display a pore structure evolution from opened to closed states, but also exhibit a surface wettability transition from hydrophilic to hydrophobic behaviors when the temperature rises. This behavior can suppress the migration of electrolyte ions across the separators, realizing the self-protection of ZIBs at high temperatures. Furthermore, the thermal-gated behavior is highly reversible, even after multiple heating/cooling cycles, because of the reversibility of temperature-dependent structural evolution and hydrophilic/hydrophobic transition. This work will pave the way for designing thermal-responsive energy-storage devices with safe and controlled energy delivery.

2.
Angew Chem Int Ed Engl ; 59(29): 11800-11807, 2020 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-32301196

RESUMO

The reversible capacity of AlCl4 - intercalation/de-intercalation in conventional cathodes of aluminum-ion batteries (AIBs) is difficult to improve due to the large size of AlCl4 - anions. Therefore, it is highly desirable to realize the intercalation/de-intercalation of smaller Al-based ions. Here, we fabricated polyaniline/single-walled carbon nanotubes (PANI/SWCNTs) composite films and protonated the PANI nanorods. The protonation endows PANI with more active sites and enhanced conductivity. Hyper self-protonated PANI (PANI(H+ )) exhibits reversible AlCl2 + intercalation/de-intercalation during the discharge/charge process. As a result, the discharge capacity of the Al/PANI(H+ ) battery is twice as high as that of the initial composite films. PANI(H+ )@SWCNT electrodes also have a stable cycling life with only 0.003 % capacity decay per cycle over 8000 cycles. Owing to the excellent mechanical properties, PANI(H+ )@SWCNT composite films can act as the electrodes of flexible AIBs.

3.
FEMS Microbiol Ecol ; 96(4)2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32109277

RESUMO

Understanding microbial network assembly is a promising way to predict potential impacts of environmental changes on ecosystem functions. Yet, soil microbial network assembly in mountain ecosystems and its underlying mechanisms remain elusive. Here, we characterized soil microbial co-occurrence networks across 12 altitudinal sites in Mountain Gongga. Despite differences in habitats, soil bacterial networks separated into two different clusters by altitude, namely the lower and higher altitudes, while fungi did not show such a pattern. Bacterial networks encompassed more complex and closer relationships at the lower altitudes, while fungi had closer relationships at the higher altitudes, which could be attributed to niche differentiation caused by high variations in soil environments and plant communities. Both abiotic and biotic factors (e.g. soil pH and bacterial community composition) shaped bacterial networks. However, biotic factors played more important roles than the measured abiotic factors for fungal network assembly. Further analyses suggest that multiple mechanisms including niche overlap/differentiation, cross-feeding and competition between microorganisms could play important roles in shaping soil microbial networks. This study reveals microbial co-occurrence networks in response to different ecological factors, which provides important insights into our comprehensive understanding of microbial network assembly and their functional potentials in mountain ecosystems.

4.
Nanoscale ; 11(38): 17630-17636, 2019 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-31538168

RESUMO

The recent development of flexible and wearable electronic devices has increased the demand for energy storage devices with excellent flexibility and structural stability. Aqueous zinc-ion batteries (ZIBs) are promising energy storage devices due to their low cost, high safety, and eco-friendliness. Therefore, flexible ZIBs have to be considered. Herein, we design the flexible all-in-one ZIBs, where the reduced graphene oxide/polyaniline (rGO/PANI) cathode, cellulose nanofiber (CNF) separator, and exfoliated graphene (EG)/Zn anode are integrated together using an all-freeze-casting strategy. The continuous seamless connection of such all-in-one ZIBs can avoid displacing and detaching between the electrodes and separator under different bending states and improve the load-transfer capacity and interface strength between the neighboring component layers. As a result, the all-in-one ZIBs show excellent flexibility and superior electrochemical stability under different bending states.

5.
ACS Nano ; 12(12): 12503-12511, 2018 Dec 26.
Artigo em Inglês | MEDLINE | ID: mdl-30507142

RESUMO

The recent boom in flexible and wearable electronic devices has increased the demand for flexible energy storage devices. The flexible lithium-sulfur (Li-S) battery is considered to be a promising candidate due to its high energy density and low cost. Herein, a flexible Li-S battery was fabricated based on an all-in-one integrated configuration, where a multiwalled carbon nanotubes/sulfur (MWCNTs/S) cathode, MWCNTs/manganese dioxide (MnO2) interlayer, polypropylene (PP) separator, and Li anode were integrated together by combining blade coating with vacuum evaporation methods. Each component of the all-in-one structure can be seamlessly connected with the neighboring layers. Such an optimal interfacial connection can effectively enhance electron- and/or load-transfer capacity by avoiding the relative displacement or detachment between two neighboring components at bending strain. Therefore, the flexible all-in-one Li-S batteries display fast electrochemical kinetics and have stable electrochemical performance under different bending states.

6.
Sci Rep ; 7(1): 17492, 2017 12 13.
Artigo em Inglês | MEDLINE | ID: mdl-29235487

RESUMO

How press disturbance (long-term) influences the phylogenetic turnover of soil microbial communities responding to pulse disturbances (short-term) is not fully known. Understanding the complex connections between the history of environmental conditions, assembly processes and microbial community dynamics is necessary to predict microbial response to perturbation. We started by investigating phylogenetic spatial turnover (based on DNA) of soil prokaryotic communities after long-term nitrogen (N) deposition and temporal turnover (based on RNA) of communities responding to pulse by conducting short-term rewetting experiments. The results showed that moderate N addition increased ecological stochasticity and phylogenetic diversity. In contrast, high N addition slightly increased homogeneous selection and decreased phylogenetic diversity. Examining the system with higher phylogenetic resolution revealed a moderate contribution of variable selection across the whole N gradient. The moisture pulse experiment showed that high N soils had higher rates of phylogenetic turnover across short phylogenetic distances and significant changes in community compositions through time. Long-term N input history influenced spatial turnover of microbial communities, but the dominant community assembly mechanisms differed across different N deposition gradients. We further revealed an interaction between press and pulse disturbances whereby deterministic processes were particularly important following pulse disturbances in high N soils.


Assuntos
Nitrogênio , Microbiologia do Solo , Água , Biodiversidade , Nitrogênio/química , Nitrogênio/metabolismo , Filogenia , RNA Ribossômico 16S , Solo/química , Análise Espaço-Temporal , Água/química , Água/metabolismo
7.
Appl Microbiol Biotechnol ; 101(19): 7303-7316, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28828628

RESUMO

Methanosarcina barkeri (DSM 800) is a metabolically versatile methanogen and shows distinct metabolic status under different substrate regimes. However, the mechanisms underlying distinct transcriptional profiles under different substrate regimes remain elusive. In this study, based on transcriptional analysis, the growth performances and gene expressions of M. barkeri fed on acetate, H2 + CO2, and methanol, respectively, were investigated. M. barkeri showed higher growth performances under methanol, followed by H2 + CO2 and acetate, which corresponded well with the variations of gene expressions. The α diversity (evenness) of gene expressions was highest under the acetate regime, followed by H2 + CO2 and methanol, and significantly and negatively correlated with growth performances. The gene co-expression analysis showed that "Energy production and conversion," "Coenzyme transport and metabolism," and "Translation, ribosomal structure, and biogenesis" showed deterministic cooperation patterns of intra- and inter-functional classes. However, "Posttranslational modification, protein turnover, chaperones" showed exclusion with other functional classes. The gene expressions and especially the relationships among them potentially drove the shifts of metabolic status under different substrate regimes. Consequently, this study revealed the diversity-related ecological strategies that a high α diversity probably provided more fitness and tolerance under natural environments and oppositely a low α diversity strengthened some specific physiological functions, as well as the co-responses of gene expressions to different substrate regimes.


Assuntos
Meios de Cultura/química , Euryarchaeota/genética , Methanosarcina barkeri/genética , Transcriptoma , Ácido Acético/química , Dióxido de Carbono/química , Euryarchaeota/metabolismo , Regulação da Expressão Gênica em Archaea , Hidrogênio/química , Concentração de Íons de Hidrogênio , Metanol/química , Methanosarcina barkeri/metabolismo , RNA Arqueal/genética , Análise de Sequência de RNA , Especificidade por Substrato
8.
Water Res ; 123: 134-143, 2017 10 15.
Artigo em Inglês | MEDLINE | ID: mdl-28662395

RESUMO

Temperature plays crucial roles in microbial interactions that affect the stability and performance of anaerobic digestion. In this study, the microbial interactions and their succession in the anaerobic digestion process were investigated at three levels, represented by (1) present and (2) active micro-organisms, and (3) gene expressions under a temperature gradient from 25 to 55 °C. Network topological features indicated a global variation in microbial interactions at different temperatures. The variations of microbial interactions in terms of network modularity and deterministic processes based on topological features, corresponded well with the variations of methane productions, but not with temperatures. A common successional pattern of microbial interactions was observed at different temperatures, which showed that both deterministic processes and network modularity increased over time during the digestion process. It was concluded that the increase in temperature-mediated network modularity and deterministic processes on shaping the microbial interactions improved the stability and efficiency of anaerobic digestion process.


Assuntos
Reatores Biológicos , Interações Microbianas , Temperatura , Anaerobiose , Metano
9.
Appl Environ Microbiol ; 83(14)2017 07 15.
Artigo em Inglês | MEDLINE | ID: mdl-28500044

RESUMO

Some bacteria are capable of forming flocs, in which bacterial cells become self-flocculated by secreted extracellular polysaccharides and other biopolymers. The floc-forming bacteria play a central role in activated sludge, which has been widely utilized for the treatment of municipal sewage and industrial wastewater. Here, we use a floc-forming bacterium, Aquincolatertiaricarbonis RN12, as a model to explore the biosynthesis of extracellular polysaccharides and the regulation of floc formation. A large gene cluster for exopolysaccharide biosynthesis and a gene encoding the alternative sigma factor RpoN1, one of the four paralogues, have been identified in floc formation-deficient mutants generated by transposon mutagenesis, and the gene functions have been further confirmed by genetic complementation analyses. Interestingly, the biosynthesis of exopolysaccharides remained in the rpoN1-disrupted flocculation-defective mutants, but most of the exopolysaccharides were secreted and released rather than bound to the cells. Furthermore, the expression of exopolysaccharide biosynthesis genes seemed not to be regulated by RpoN1. Taken together, our results indicate that RpoN1 may play a role in regulating the expression of a certain gene(s) involved in the self-flocculation of bacterial cells but not in the biosynthesis and secretion of exopolysaccharides required for floc formation.IMPORTANCE Floc formation confers bacterial resistance to predation of protozoa and plays a central role in the widely used activated sludge process. In this study, we not only identified a large gene cluster for biosynthesis of extracellular polysaccharides but also identified four rpoN paralogues, one of which (rpoN1) is required for floc formation in A. tertiaricarbonis RN12. In addition, this RpoN sigma factor regulates the transcription of genes involved in biofilm formation and swarming motility, as previously shown in other bacteria. However, this RpoN paralogue is not required for the biosynthesis of exopolysaccharides, which are released and dissolved into culture broth by the rpoN1 mutant rather than remaining tightly bound to cells, as observed during the flocculation of the wild-type strain. These results indicate that floc formation is a regulated complex process, and other yet-to-be identified RpoN1-dependent factors are involved in self-flocculation of bacterial cells via exopolysaccharides and/or other biopolymers.


Assuntos
Proteínas de Bactérias/metabolismo , Betaproteobacteria/metabolismo , Polissacarídeos Bacterianos/biossíntese , Fator sigma/metabolismo , Proteínas de Bactérias/genética , Betaproteobacteria/química , Betaproteobacteria/genética , Floculação , Regulação Bacteriana da Expressão Gênica , Fator sigma/genética
10.
FEMS Microbiol Ecol ; 93(4)2017 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-28334191

RESUMO

Temperate steppe is extremely sensitive to the current global changes. However, what are the main environmental variables driving microbial diversity in temperate steppe are still unclear, something that impairs doing predictions about the expected effects of global changes on microbe-mediated ecological functions. This is why, in this study, the relationship between soil microbial diversity and environmental variables in Chinese temperate steppe is investigated. In this study, significant correlations between soil bacterial α-diversity and mean annual precipitation and the aridity index were observed at the whole region scale. No clear correlations between microbial α-diversities and other measured environmental variables were found at the whole temperate steppe region and sub-regions. On the other hand, ß-diversity was strongly related to spatial variables and climate variables for bacteria, while spatial variables and soil organic matters were more related with fungal ß-diversity. In addition, the mean annual temperature was highly correlated with microbial ß-diversity at different spatial scales, suggesting that it could be a good single predictor of soil microbial assemblage in temperate steppe. ß-Diversities are more explained by combined effect of local environmental variables based on variation partitioning analysis, reflecting the community assemblage is more likely driven by species sorting through environmental filtering.


Assuntos
Biodiversidade , Variação Genética , Microbiologia do Solo , Bactérias/classificação , Bactérias/genética , China , Clima , Ecossistema , Fungos/classificação , Fungos/genética , Solo , Temperatura
11.
Nanoscale ; 9(14): 4646-4651, 2017 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-28327706

RESUMO

Freestanding reduced graphene oxide-sulfur (rGO-S) composite films were fabricated by combining solution infiltration of sulfur into solvated rGO films with freeze-drying. Such rGO-S composite films can directly serve as the cathodes of lithium-sulfur (Li-S) batteries. The nanostructured architecture of rGO-S composite films considerably improved the cycling stability of Li-S batteries.

12.
FEMS Microbiol Ecol ; 93(3)2017 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-28137765

RESUMO

The gut microbial communities of animals play key roles in host evolution. However, the possible relationship between gut microbiota and host divergence time remains unknown. Here, we investigated the gut microbiota of eight Glires species (four lagomorph species and four rodent species) distributed throughout the Qinghai-Tibet plateau and Inner Mongolia grassland. Lagomorphs and rodents had distinct gut microbial compositions. Three out of four lagomorph species were dominated by Firmicutes, while rodents were dominated by Bacteroidetes in general. The alpha diversity values (Shannon diversity and evenness) exhibited significant differences between any two species within the lagomorphs, whereas there were no significant differences among rodents. The structure of the gut microbiota showed significant differences between lagomorphs and rodents. In addition, we calculated host phylogeny and divergence times, and used a phylogenetic approach to reconstruct how the animal gut microbiota has diverged from their ancestral species. Some core bacterial genera (e.g. Prevotella and Clostridium) shared by more than nine-tenths of all the Glires individuals associated with plant polysaccharide degradation showed marked changes within lagomorphs. Differences in Glires gut microbiota (based on weighted UniFrac and Bray-Curtis dissimilarity metrics) were positively correlated with host divergence time. Our results thus suggest the gut microbial composition is associated with host phylogeny, and further suggest that dissimilarity of animal gut microbiota may predict host divergence time.


Assuntos
Microbioma Gastrointestinal , Trato Gastrointestinal/microbiologia , Animais , Bactérias/classificação , Bacteroidetes/classificação , Evolução Biológica , China , Microbiota , Filogenia , Tibet
13.
Front Microbiol ; 7: 1269, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27582734

RESUMO

The composition of the mammalian gut bacterial communities can be influenced by the introduction of environmental bacteria in their respective habitats. However, there are no extensive studies examining the interactions between environmental bacteriome and gut bacteriome in wild mammals. Here, we explored the relationship between the gut bacterial communities of pika (Ochotona spp.) and the related environmental bacteria across host species and altitudinal sites using 16S rRNA gene sequencing. Plateau pikas (O. curzoniae) and Daurian pikas (O. daurica) were sampled at five different sites, and plant and soil samples were collected at each site as well. Our data indicated that Plateau pikas and Daurian pikas had distinct bacterial communities. The pika, plant and soil bacterial communities were also distinct. Very little overlap occurred in the pika core bacteria and the most abundant environmental bacteria. The shared OTUs between pikas and environments were present in the environment at relatively low abundance, whereas they were affiliated with diverse bacterial taxa. These results suggested that the pika gut may mainly select for low-abundance but diverse environmental bacteria in a host species-specific manner.

14.
FEMS Microbiol Ecol ; 92(3)2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26880783

RESUMO

To understand the temporal responses of soil prokaryotic communities to clear-cutting disturbance, we examined the changes in soil bacterial and archaeal community composition, structure and diversity along a chronosequence of forest successional restoration using high-throughput 16S rRNA gene sequencing. Our results demonstrated that clear-cutting significantly altered soil bacterial community structure, while no significant shifts of soil archaeal communities were observed. The hypothesis that soil bacterial communities would become similar to those of surrounding intact primary forest with natural regeneration was supported by the shifts in the bacterial community composition and structure. Bacterial community diversity patterns induced by clear-cutting were consistent with the intermediate disturbance hypothesis. Dynamics of bacterial communities was mostly driven by soil properties, which collectively explained more than 70% of the variation in bacterial community composition. Community assembly data revealed that clear-cutting promoted the importance of the deterministic processes in shaping bacterial communities, coinciding with the resultant low resource environments. But assembly processes in the secondary forest returned a similar level compared to the intact primary forest. These findings suggest that bacterial community dynamics may be predictable during the natural recovery process.


Assuntos
Bactérias/isolamento & purificação , Microbiologia do Solo , Bactérias/classificação , Bactérias/genética , Florestas , Dados de Sequência Molecular , Filogenia , RNA Ribossômico 16S/genética , Solo/química , Árvores/crescimento & desenvolvimento , Árvores/microbiologia
15.
Front Microbiol ; 6: 1337, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26648921

RESUMO

The microbial-mediated anaerobic digestion (AD) process represents an efficient biological process for the treatment of organic waste along with biogas harvest. Currently, the key factors structuring bacterial communities and the potential core and unique bacterial populations in manure anaerobic digesters are not completely elucidated yet. In this study, we collected sludge samples from 20 full-scale anaerobic digesters treating cattle or swine manure, and investigated the variations of bacterial community compositions using high-throughput 16S rRNA amplicon sequencing. Clustering and correlation analysis suggested that substrate type and free ammonia (FA) play key roles in determining the bacterial community structure. The COD: [Formula: see text] (C:N) ratio of substrate and FA were the most important available operational parameters correlating to the bacterial communities in cattle and swine manure digesters, respectively. The bacterial populations in all of the digesters were dominated by phylum Firmicutes, followed by Bacteroidetes, Proteobacteria and Chloroflexi. Increased FA content selected Firmicutes, suggesting that they probably play more important roles under high FA content. Syntrophic metabolism by Proteobacteria, Chloroflexi, Synergistetes and Planctomycetes are likely inhibited when FA content is high. Despite the different manure substrates, operational conditions and geographical locations of digesters, core bacterial communities were identified. The core communities were best characterized by phylum Firmicutes, wherein Clostridium predominated overwhelmingly. Substrate-unique and abundant communities may reflect the properties of manure substrate and operational conditions. These findings extend our current understanding of the bacterial assembly in full-scale manure anaerobic digesters.

16.
Artigo em Inglês | MEDLINE | ID: mdl-24109352

RESUMO

The title compound, C16H16BrNO4, has two adjacent chiral C atoms and both have an S configuration. The fused cyclo-hex-2-enone and di-hydro-furan rings both adopt envelope conformations, with the quaternary C atom and the nitro-substituted C atoms as the respective flap. The flap atoms lie 0.607 (3) and -0.253 (2) Å, respectively, from the mean plane of the remaining ring atoms on opposite sides. The dihedral angle between the mean plane of the four coplanar atoms of the di-hydro-furan ring and the phenyl ring is 86.16 (3)°. In the crystal, mol-ecules are linked by weak C-H⋯O inter-actions, forming a ladder motif parallel to the b axis.

17.
Acta Crystallogr Sect E Struct Rep Online ; 69(Pt 5): o785, 2013 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-23723929

RESUMO

In the title compound, C20H25NO5, the di-hydro-pyridine ring adopts a flattened boat conformation. The meth-oxy-phenyl ring is almost perpendicular to the mean plane of the pyridine ring [dihedral angle = 88.42 (3)°]. The two carbonyl units adopt a synperiplanar conformation with respect to the double bonds in the di-hydro-pyridine ring. In the crystal, mol-ecules are connected by N-H⋯O hydrogen bonds into R 4 (4)(24) tetra-meric rings. A region of disordered electron density, located at the center of four adjacent mol-ecules, was treated with the SQUEEZE routine in PLATON [Spek (2009 ▶). Acta Cryst. D65, 148-155]. It is probably the result of traces of the solvent of crystallization and was not taken into account during the structure refinement.

18.
J Environ Sci (China) ; 25(12): 2422-8, 2013 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-24649673

RESUMO

Although microbial treatments of heavy metal ions in wastewater have been studied, the removal of these metals through incorporation into carbonate minerals has rarely been reported. To investigate the removal of Fe3+ and Pb2+, two representative metals in wastewater, through the precipitation of carbonate minerals by a microbial flocculant (MBF) produced by Bacillus mucilaginosus. MBF was added to synthetic wastewater containing different Fe3+ and Pb2+ concentrations, and the extent of flocculation was analyzed. CO2 was bubbled into the mixture of MBF and Fe3+/Pb2+ to initiate the reaction. The solid substrates were analyzed via X-ray diffraction, transmission electron microscopy and energy dispersive spectroscopy. The results showed that the removal efficiency decreased and the MBF adsorption capacity for metals increased with increasing heavy metal concentration. In the system containing MBF, metals (Fe3+ and Pb2+), and CO2, the concentrated metals adsorbed onto the MBF combined with the dissolved CO2, resulting in oversaturation of metal carbonate minerals to form iron carbonate and lead carbonates. These results may be used in designing a method in which microbes can be utilized to combine CO2 with wastewater heavy metals to form carbonates, with the aim of mitigating environmental problems.


Assuntos
Bacillus/fisiologia , Carbonatos/síntese química , Ferro/isolamento & purificação , Chumbo/isolamento & purificação , Águas Residuárias/química , Adsorção , Dióxido de Carbono/química , Precipitação Química , Microscopia Eletrônica de Transmissão , Espectrometria por Raios X , Eliminação de Resíduos Líquidos , Difração de Raios X
19.
Ying Yong Sheng Tai Xue Bao ; 18(8): 1921-4, 2007 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-17974268

RESUMO

By using simulation method, this paper studied the effects of furadan on the activities of soil urease, invertase and alkaline phosphatase, with the affecting factors investigated. The results showed that after adding furadan into soil, soil urease activity was decreased first but increased then, indicating that the ecotoxicity of furadan was reduced gradually. When the concentration of furadan was less than 0.3%, soil urease, invertase and alkaline phosophatase were all activated, and in some soil samples, a significant positive correlation was observed between soil invertase activity and furadan concentration, suggesting that soil invertase activity could be used as an indicator for the soil pollution caused by furadan. It was concluded that the quality of soil and ecological environment would be kept safe under low concentrations of furadan (< or = 0.3%).


Assuntos
Carbofurano/toxicidade , Solo/análise , beta-Frutofuranosidase/metabolismo , Fosfatase Alcalina/antagonistas & inibidores , Fosfatase Alcalina/metabolismo , Inibidores da Colinesterase/toxicidade , Relação Dose-Resposta a Droga , Ativação Enzimática/efeitos dos fármacos , Urease/antagonistas & inibidores , Urease/metabolismo , beta-Frutofuranosidase/antagonistas & inibidores
20.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 19(1): 84-5, 2003 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-15132917

RESUMO

AIM: To prepare monoclonal antibody(mAb) against humen laminin(LN) and identify their properties. METHODS: BALB/c mice were immunized with humen LN, and then mAb was prepared by hybridoma technique. The titres of mAbs in ascetic fluid and relative affinity of mAb were determined by indirect ELISA test. Ig subgroup, specificity and combine dot of mAb were analysed by ELISA. RESULTS: 4 hybridoma cell lines 2A3, 2C6, 3G7 and 4H2 secreting anti-humen LN mAb were obtained. Their Ig subgroup belonged to IgG1, light chains were type kappa, The titres of 4 mAbs in ascitic fluid were 3.6 x 10(4)-2.1 x 10(6). Relative affinity of mAb 2C6 was above 10(12) and relative affinity of other 3 mAbs 2A3, 3G7 and 4H2 was 10(6), 10(8) and 10(8). Epitope recognized by 2 mAb(2A3 and 4H2) differed from the that by 2C6 and 3G7 another. CONCLUSION: 4 mAbs against humen LN has been prepared successfully and possess higher titer, specificity and relative affinity. Preparation of anti-human LN mAbs provide a useful reagent for further studying the role of LN in some diseases.


Assuntos
Anticorpos Monoclonais/biossíntese , Laminina/imunologia , Animais , Anticorpos Monoclonais/imunologia , Especificidade de Anticorpos , Humanos , Hibridomas/imunologia , Hibridomas/metabolismo , Imunoglobulina G/biossíntese , Imunoglobulina G/imunologia , Cadeias kappa de Imunoglobulina/biossíntese , Cadeias kappa de Imunoglobulina/imunologia , Camundongos , Camundongos Endogâmicos BALB C
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