Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 25
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Gene ; 702: 182-193, 2019 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-30910561

RESUMO

Programmed death-1 (PD-1) has a pivotal role in the attenuation of adaptive immune responses and peripheral tolerance. Here we describe the identification of the Pekin duck programmed death-1 orthologue (duPD-1). The duPD-1 cDNA encodes a 283-amino acid polypeptide that has an amino acid identity of 70%, 32% and 31% with chicken, murine and human PD-1, respectively. The duck PD-1 gene shares five conserved exons with chicken, murine and human PD-1 genes. A cluster of putative regulatory elements within the conserved region B (CR-B) of the basal promotor is conserved. Homology modeling was most compatible with the two ß-sheet IgV domain structure of murine PD-1. Contact residues, shown to be critical for binding of the respective human and murine PD-1 ligands are mostly conserved between avian and mammalian species, whereas residues that define the cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) are highly conserved across higher vertebrates and frog. Constitutive expression of duPD-1 transcripts was predominantly found in lymphocyte-rich tissues, and mitogen-stimulation of duck peripheral blood mononuclear cells transiently increased duPD-1 mRNA expression. A soluble duPD-1 protein was expressed and shown to engage the identified duck PD-1 ligands. Our observations show considerable evolutionary conservation between mammalian and avian PD-1 orthologues. This work will facilitate further investigation of the role of PD-1 signaling in adaptive immunity in the Pekin duck, a non-mammalian vertebrate and pathogen host with relevance for human and animal health.


Assuntos
Proteínas Aviárias/química , Proteínas Aviárias/genética , Receptor de Morte Celular Programada 1/química , Receptor de Morte Celular Programada 1/genética , Animais , Proteínas Aviárias/classificação , Mapeamento Cromossômico , Clonagem Molecular , Patos , Expressão Gênica , Ligantes , Modelos Moleculares , Filogenia , Receptor de Morte Celular Programada 1/classificação , Receptor de Morte Celular Programada 1/metabolismo , Domínios Proteicos , Estrutura Secundária de Proteína , RNA Mensageiro/metabolismo , Alinhamento de Sequência , Análise de Sequência de Proteína , Distribuição Tecidual
2.
Dalton Trans ; 48(16): 5144-5148, 2019 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-30919864

RESUMO

A microporous metal-organic framework functionalized with in situ generated NHC-CuCl units (1) has been successfully synthesized from a novel imidazolium-containing ligand. In particular, the MOF 1 can catalyze ß-boration of α,ß-unsaturated carbonyl compounds, while the isoreticular version of 1 (1-im) modified with only imidazolium moiety cannot. This work demonstrates for the first time the heterogeneous catalysis of NHC-Cu(i)Cl within a MOF solid.

3.
Biochem Biophys Rep ; 13: 116-122, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29556566

RESUMO

Programmed death-1 (PD-1), upon engagement by its ligands, programmed death ligand-1 (PD-L1) and programmed death ligand-2 (PD-L2), provides signals that attenuate adaptive immune responses. Here we describe the identification of the Pekin duck PD-L2 (duPD-L2) and its gene structure. The duPD-L2 cDNA encodes a 321 amino acid protein that has an amino acid identity of 76% and 35% with chicken and human PD-L2, respectively. Mapping of the duPD-L2 cDNA with duck genomic sequences revealed an exonic structure similar to that of the human Pdcd1lg2 gene. Homology modelling of the duPD-L2 protein was compatible with the murine PD-L2 ectodomain structure. Residues known to be important for PD-1 receptor binding of murine PD-L2 were mostly conserved in duPD-L2 within sheets A and G and partially conserved within sheets C and F. DuPD-L2 mRNA was constitutively expressed in all tissues examined with highest expression levels in lung, spleen, cloaca, bursa, cecal tonsil, duodenum and very low levels of expression in muscle, kidney and brain. Lipopolysaccharide treatment of adherent duck PBMC upregulated duPD-L2 mRNA expression. Our work shows evolutionary conservation of the PD-L2 ectodomain structure and residues important for PD-1 binding in vertebrates including fish. The information provided will be useful for further investigation of the role of duPD-L2 in the regulation of duck adaptive immunity and exploration of PD-1-targeted immunotherapies in the duck hepatitis B infection model.

4.
Chem Commun (Camb) ; 53(15): 2394-2397, 2017 Feb 16.
Artigo em Inglês | MEDLINE | ID: mdl-28174776

RESUMO

Two Fe-based porous MOFs have been constructed from dimeric Fe-clusters and rod-shaped heterobimetallic {Fe2Na3}n chains as SBUs, respectively. Both of them exhibit highly selective CO2 adsorption over CH4 and N2, owing to their abundant multiple active sites.

5.
Dalton Trans ; 43(45): 16986-91, 2014 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-25315719

RESUMO

Reaction of Mn(OAc)2·4H2O with the H2L ligand affords a new manganese 22-MC-8 azametallacrown [Mn8(µ3-O)2(µ3-OH)2(µ2-OH)2(L)6(OAc)2(OH2)4]·2DMF (H2L = 3-(2-oxyphenyl)-5-(pyrazin-2-yl)-1,2,4-triazole). The structural analysis of the complex reveals that the azaMC ring possesses -[Mn-O-Mn-N-N-Mn-N-N-Mn-N-N]- connectivity. The magnetic behavior of the complex shows the dominant antiferromagnetic interactions between the manganese ions with S = 4 and the frequency-dependent out-of-phase signal.

6.
Chemistry ; 20(45): 14846-52, 2014 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-25234745

RESUMO

Three isoreticular hydrogen-bonded frameworks with functionalized pore structures were constructed by a modular self-assembly process in which a series of amino acids with various substituents serve as facile exchange subassemblies to decorate the pore wall. The ordered amino acid side-chain groups in the pore channels play an important role in determining the adsorption behavior of the framework materials, and ensure exclusive adsorption of methanol/water over ethanol. Gas-chromatographic separation experiments demonstrated that alcohols can be efficiently separated from ternary water/methanol/ethanol mixtures and revealed a key influence of the adsorbate-host framework interaction on the practical separation performance of mixtures.

7.
Gene ; 548(1): 29-38, 2014 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-24992029

RESUMO

Interferons (IFNs) are the first line of defense against viral infections in vertebrates. Type III interferon (IFN-λ) is recognized for its key role in innate immunity of tissues of epithelial origin. Here we describe the identification of the Pekin duck IFN-λ ortholog (duIFN-λ). The predicted duIFN-λ protein has an amino acid identity of 63%, 38%, 37% and 33% with chicken IFN-λ and human IFN-λ3, IFN-λ2 and IFN-λ1, respectively. The duck genome contains a single IFN-λ gene that is comprised of five exons and four introns. Recombinant duIFN-λ up-regulated OASL and Mx-1 mRNA in primary duck hepatocytes. Our observations suggest evolutionary conservation of genomic organization and structural features implicated in receptor binding and antiviral activity. The identification and expression of duIFN-λ will facilitate further study of the role of type III IFN in antiviral defense and inflammatory responses of the Pekin duck, a non-mammalian vertebrate and pathogen host with relevance for human and animal health.


Assuntos
Patos/genética , Interferons/genética , Interferons/metabolismo , 2',5'-Oligoadenilato Sintetase/genética , 2',5'-Oligoadenilato Sintetase/metabolismo , Sequência de Aminoácidos , Animais , Galinhas/genética , Clonagem Molecular , Éxons , Regulação da Expressão Gênica , Hepatócitos/fisiologia , Interferons/química , Interleucinas/genética , Íntrons , Modelos Moleculares , Dados de Sequência Molecular , Proteínas de Resistência a Myxovirus/genética , Filogenia , Conformação Proteica , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Homologia de Sequência de Aminoácidos
8.
Angew Chem Int Ed Engl ; 53(14): 3608-11, 2014 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-24590597

RESUMO

A new porous vanadoborate was synthesized by employing the scale chemistry theory with the vanadoborate cluster V10B28. The twofold interpenetrated lvt network was assembled with zinc-containing elliptical vanadoborate clusters and Zn polyhedra. The single lvt framework contains a three-dimensional 38×38×20 ring channel system with the pore size (24.7×12.7 Å) reaching the mesoscale, thus indicating the possibility of constructing 3D ordered mesopores with vanadoborate clusters. The porosity of the SUT-7 structure was confirmed by CO2 adsorption of the as-synthesized materials.

9.
Chemistry ; 19(51): 17483-93, 2013 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-24265270

RESUMO

Palladium nanoparticles have been immobilized into an amino-functionalized metal-organic framework (MOF), MIL-101Cr-NH2, to form Pd@MIL-101Cr-NH2. Four materials with different loadings of palladium have been prepared (denoted as 4-, 8-, 12-, and 16 wt%Pd@MIL-101Cr-NH2). The effects of catalyst loading and the size and distribution of the Pd nanoparticles on the catalytic performance have been studied. The catalysts were characterized by using scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier-transform infrared (FTIR) spectroscopy, powder X-ray diffraction (PXRD), N2-sorption isotherms, elemental analysis, and thermogravimetric analysis (TGA). To better characterize the palladium nanoparticles and their distribution in MIL-101Cr-NH2, electron tomography was employed to reconstruct the 3D volume of 8 wt%Pd@MIL-101Cr-NH2 particles. The pair distribution functions (PDFs) of the samples were extracted from total scattering experiments using high-energy X-rays (60 keV). The catalytic activity of the four MOF materials with different loadings of palladium nanoparticles was studied in the Suzuki-Miyaura cross-coupling reaction. The best catalytic performance was obtained with the MOF that contained 8 wt% palladium nanoparticles. The metallic palladium nanoparticles were homogeneously distributed, with an average size of 2.6 nm. Excellent yields were obtained for a wide scope of substrates under remarkably mild conditions (water, aerobic conditions, room temperature, catalyst loading as low as 0.15 mol%). The material can be recycled at least 10 times without alteration of its catalytic properties.

10.
Dev Comp Immunol ; 38(1): 30-43, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22469657

RESUMO

Here we describe the cloning and expression of Pekin duck IL-10 (duIL-10) and a six exon-5 intron structure of an IL-10 gene. Two transcripts encoding duIL-10 with an alternatively spliced 3'UTR, and a transcript lacking exon 5 with a novel coding sequence for its C-terminus (duIL-10ΔE5) were isolated from splenocytes. The duIL-10 protein has an amino acid identity of 79% and 47% with chicken and human IL-10, respectively. The duck IL-10 gene shares a similar structure of the respective exons 1-5 with the IL-10 genes of other vertebrates but has an alternative exon. The duIL-10 3D structure by homology modeling was similar to that of the human IL-10 monomer, whereas the predicted duIL-10ΔE5 protein lacks helix F. DuIL-10 and duIL-10ΔE5 transcripts were most abundant in primary and secondary immune organs and lung. Recombinant duIL-10 suppressed duck IL-2 transcripts in mitogen-activated PBMCs. Our observation suggests evolutionary conservation of structure and function of the duIL-10 protein but the roles of the novel IL-10 splice variants in the regulation of duck immune responses and evolution of vertebrate immunity remain to be elucidated.


Assuntos
Patos/genética , Patos/imunologia , Interleucina-10/genética , Processamento Alternativo , Animais , Sequência de Bases , Humanos , Interleucina-10/química , Interleucina-10/imunologia , Modelos Moleculares , Dados de Sequência Molecular , Filogenia , Alinhamento de Sequência
11.
Dalton Trans ; 41(14): 3953-5, 2012 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-22301720

RESUMO

A series of metal-organic frameworks representing a non-interpenetrated framework analogue of MOF-14 have been synthesized by using two different linkers, 4,4',4''-benzene-1,3,5-triyl-benzoic acid (H(3)BTB) and 4,4'-bipyridine (Bpy). Interestingly, the transition metal ions in the paddle-wheel metal clusters could be exchanged by other transition metal ions via a direct single-crystal to single-crystal transformation. This post-synthesis route can be used for synthesis of isomorphous metal-organic frameworks that cannot be obtained by direct synthesis.

12.
Chemistry ; 18(7): 1924-31, 2012 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-22267182

RESUMO

A series of metal-organic frameworks based on a flexible, highly charged Bpybc ligand, namely 1⋅Mn⊃OH(-), 2⋅Mn⊃SO(4)(2-), 3⋅Mn⊃bdc(2-), 4⋅Eu⊃SO(4)(2-) (H(2)BpybcCl(2) = 1,1'-bis(4-carboxybenzyl)-4,4'-bipyridinium dichloride, H(2)bdc = 1,4-benzenedicarboxylic acid) have been obtained by a self-assembly process. Single-crystal X-ray-diffraction analysis revealed that all of these compounds contained the same n-fold 2D→3D Borromean-entangled topology with irregular butterfly-like pore channels that were parallel to the Borromean sheets. These structures were highly tolerant towards various metal ions (from divalent transition metals to trivalent lanthanide ions) and anion species (from small inorganic anions to bulky organic anions), which demonstrated the superstability of these Borromean linkages. This non-interpenetrated entanglement represents a new way of increasing the stability of the porous frameworks. The introduction of bipyridinium molecules into the porous frameworks led to the formation of cationic surface, which showed high affinities to methanol and water vapor. The distinct adsorption and desorption isotherms of methanol vapor in four complexes revealed that the accommodated anion species (of different size, shape, and location) provided a unique platform to tune the environment of the pore space. Measurements of the adsorption of various organic vapors onto framework 1⋅Mn⊃OH(-) further revealed that these pores have a high adsorption selectivity towards molecules with different sizes, polarities, or π-conjugated structures.

13.
Vaccine ; 28(51): 8147-56, 2010 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-20937323

RESUMO

The potential of CD154 (CD40L) as a powerful immunological adjuvant has been shown in various strategies. In this study we examine the immunogenicity and protective efficacy of a CD40-targeting avian influenza hemagglutinin (HA) subunit DNA vaccine in ducks. DNA constructs encoded the ectodomain of the HA protein of LPAI A/mallard/BC/373/2005 (H5N2) with or without fusion to the ectodomain of duck CD154. CD40-targeting significantly accelerated and enhanced humoral responses to the vector-encoded HA protein. In viral challenge experiments with A/chicken/Vietnam/14/2005 (H5N1), DNA immunization conferred partial protection against the genetically distant HPAI. The observed improved kinetics and magnitude of immune induction suggest that CD40-targeting holds promise for influenza A vaccine development.


Assuntos
Ligante de CD40/imunologia , Glicoproteínas de Hemaglutininação de Vírus da Influenza/imunologia , Vacinas contra Influenza/imunologia , Influenza Aviária/prevenção & controle , Vacinas de DNA/imunologia , Estruturas Animais/patologia , Animais , Anticorpos Antivirais/sangue , Ligante de CD40/genética , Proteção Cruzada , Patos , Glicoproteínas de Hemaglutininação de Vírus da Influenza/genética , Histocitoquímica , Vacinas contra Influenza/genética , Influenza Aviária/patologia , Microscopia , Testes de Neutralização , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/imunologia , Vacinas de DNA/genética , Vacinas de Subunidades/genética , Vacinas de Subunidades/imunologia , Vacinas Sintéticas/genética , Vacinas Sintéticas/imunologia
14.
Dev Comp Immunol ; 34(7): 749-58, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20156479

RESUMO

Cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4, CD152) is an inhibitory T cell receptor predominately expressed on activated T cells. The duck CTLA-4 (DuCTLA-4) cDNA and a transcript lacking the predicted transmembrane encoding region (DuCTLA-4DeltaTM) were isolated from splenocytes using RT-PCR. The predicted DuCTLA-4 protein showed an identity of 92%, 49% and 47% with chicken, human and mouse homologues, respectively. Sequence comparison revealed conservation of residues implicated in the B7 ligand binding, disulfide linkages, glycosylation and intracellular signaling. DuCTLA-4 mRNA was predominately expressed in primary and secondary immune organs. DuCTLA-4 and DuCTLA-4DeltaTM transcripts were differentially regulated in PBMCs. Flow cytometric analysis showed constitutive expression of DuCTLA-4 protein on freshly isolated PBMCs and a modest increase upon mitogen stimulation. Our observations suggest that DuCTLA-4 and its isoform DuCTLA-4DeltaTM evolved before the divergence of birds and mammals. Both DuCTLA-4 isoforms have significant structural homology to mammalian CTLA-4 proteins but their individual roles in the regulation of duck immune responses remains to be elucidated.


Assuntos
Antígenos CD/isolamento & purificação , Patos/imunologia , Imunidade Adaptativa/imunologia , Sequência de Aminoácidos , Animais , Antígenos CD/genética , Antígenos CD/imunologia , Sequência de Bases , Antígeno CTLA-4 , Clonagem Molecular , Patos/genética , Camundongos , Camundongos Endogâmicos BALB C , Dados de Sequência Molecular , Filogenia , Isoformas de Proteínas , RNA Mensageiro/química , RNA Mensageiro/genética , Técnica de Amplificação ao Acaso de DNA Polimórfico , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Proteínas Recombinantes/isolamento & purificação , Alinhamento de Sequência , Análise de Sequência de DNA
15.
Chemistry ; 15(44): 11890-7, 2009 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-19780117

RESUMO

A multifunctional pillared-layer porous coordination polymer, {[Mn(2)(Bpybc)(ox)(2)]8 H(2)O}(n), has been constructed based on a flexible viologen derivative, 1,1'-bis(4-carboxybenzyl)-4,4'-bipyridinium dichloride (H(2)BpybcCl(2)), and an oxalate (ox) coligand. Single-crystal X-ray analysis reveals that the compound possesses multichannels with dimensions of about 6.1x6.6 A along the [110] and [-110] directions and 4.2x7.6 A along [100], and a void space of about 41.4 %. Hydrogen adsorption measurements at 77 K and 1 atm indicate that the compound exhibits a hydrogen uptake of 0.71 wt %. Owing to the incorporation of bipyridinium acceptor units, the compound can selectively accommodate aromatic donors into its nanosized pores based on charge-transfer interactions in an elastic way, and afford a specific color to different guests. Furthermore, the effect of perturbation exerted by the guest molecules on its magnetic properties has been investigated. The results indicate that the donor inclusion has little effect on its antiferromagnetic behavior, whereas dehydration of the compound decreases the strength of the magnetic exchange couplings and results in a change of the antiferromagnetic transition temperature from 14.7 to 9.8 K.

16.
J Virol Methods ; 160(1-2): 22-8, 2009 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-19464739

RESUMO

In this study, a DNA-launched subgenomic replicon of porcine reproductive and respiratory syndrome virus (PRRSV) was developed for use as a vaccine vector. This replicon plasmid contained a PRRSV subgenome without structural genes ORF2-ORF6, and was under the transcriptional control of the immediate-early promoter of cytomegalovirus (CMV). Using enhanced green fluorescent protein (EGFP) as a reporter gene, the DNA-launched subgenomic replicon of PRRSV, named pOK-Clone20-rep, could express heterologous genes in vitro. After direct inoculation of pOK-Clone20-rep, mice developed antibody responses that were specific for both the EGFP and the N gene in a dose-dependent manner. Furthermore, mice immunized with pOK-Clone20-rep at a dose of 100microg showed significantly enhanced levels of IFN-gamma compared with those inoculated with 100microg of pcD-EGFP, a conventional DNA vaccine that encodes EGFP. In summary, the results show that the DNA-launched subgenomic replicon of PRRSV could not only mediate foreign gene expression in vitro but also induced an immune response in vivo. Similarly, expression and immunogenicity of the N gene also strengthened the potential of the replicon to serve as a vaccine vector expressing multiple genes. It therefore provides a useful tool for vaccine development and the study of the transcription and replication of PRRSV.


Assuntos
Vetores Genéticos , Vírus da Síndrome Respiratória e Reprodutiva Suína/genética , Replicon , Vacinas Virais/genética , Vacinas Virais/imunologia , Animais , Citomegalovirus/genética , Feminino , Genes Reporter , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/imunologia , Interferon gama/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Plasmídeos , Vacinas de DNA/genética , Vacinas de DNA/imunologia , Proteínas Virais/genética , Proteínas Virais/imunologia
17.
Inorg Chem ; 48(4): 1266-8, 2009 Feb 16.
Artigo em Inglês | MEDLINE | ID: mdl-19166285

RESUMO

A novel coordination compound, {[Cd(BDC)(Bpybc)(1.5)].10H(2)O}(n), obtained by the reaction of CdCl(2) with 1,1'-bis(4-carboxybenzyl)-4,4'-bipyridinium dichloride (H(2)BpybcCl(2)) and 1,4-benzenedicarboxylic acid (H(2)BDC), contains 1D polymeric chains that are comprised of alternating rings and rods and dangling lateral arms. These 1D polymeric motifs are interlaced via rotaxane-like mechanical linkages to give 2D armed-polyrotaxane sheets, which are further mutually polythreaded via pseudorotaxane-like mechanical linkages to form a 3D polypseudorotaxane array. Notably, a sandwich-type donor-acceptor-donor stacking is formed within each ring as a consequence of both types of polythreading in this species, and photoinduced and thermal-induced reduction of bipyridinium occurs with a color change from light yellow to blue.

18.
Chem Commun (Camb) ; (1): 59-61, 2009 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-19081998

RESUMO

A unique supramolecular helix assisted by water tetramers is obtained by static evaporation of aqueous solution of a zwitterionic salt, 1,1'-bis(4-carboxylatebenzyl)-4,4'-bipyridinium; spontaneous chiral symmetry breaking occurs in crystallization; interestingly, redox chromism behavior of crystals upon exposure to alcohol atmosphere has been observed.


Assuntos
Compostos de Piridínio/química , Dicroísmo Circular , Cristalização , Ligação de Hidrogênio , Luz , Modelos Moleculares , Conformação Molecular , Oxirredução , Polímeros/química , Substâncias Redutoras , Estereoisomerismo , Viologênios/química , Água/química
19.
Dalton Trans ; (3): 355-62, 2008 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-18411845

RESUMO

Three hybrid host-guest compounds {(MV)2[Ni(SCN)5].Cl.2H2O}n (1), {(MV)[Mn(N3)2(SCN)2]}n (2) and {(MV)[Co(N3)2(SCN)2}n (3) (where MV2+ = methylviologen dication) have been obtained by self-assembly methods and characterized by X-ray crystallography, spectral methods and magnetic measurements. Compound 1 shows a quasi-two-dimensional structure which is formed by novel single thiocyanate-bridged Ni(II) chains connected through S...S interactions. Compounds 2 and 3 are isostructural, containing single micro(1,3)-azide bridged Mn(II)/Co(II) square layers. MV2+, as a strong electron-acceptor and a template, is embedded between the anionic layers in all three compounds. The charge-transfer (CT) interactions between MV2+ and the anionic hosts have been revealed by structural analysis and UV-vis diffuse reflection spectroscopy. The magnetic studies of the compounds show antiferromagnetic interactions between adjacent metal ions (J = -34.52(7) cm(-1) for 1, J = -3.90(2) cm(-1) for 2 and J = -10.96(6) cm(-1) for 3).

20.
Virus Genes ; 36(2): 393-400, 2008 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18246420

RESUMO

Foot-and-mouth disease (FMD) is the most contagious and devastating disease of livestock. Our previous studies demonstrated that TK(-)/gG(-)/P1(+), a recombinant expressing the FMDV capsid precursor protein (P1) based on attenuated pseudorabies virus (PRV) TK(-)/gG(-), could be used as a recombinant vaccine to protect pigs against both pseudorabies and FMD. However, because the P1 expression cassette is inserted into the gG locus of the genome of PRV TK(-)/gG(-), this bivalent vaccine cannot be used in conjunction with the PRV gE-ELISA, an extensively used discriminatory serological test in eradication programs for pseudorabies, which limits the clinical use of this bivalent vaccine. To circumvent this shortcoming, in this study, an expression cassette containing synthetic multiepitope gene "FHG" consisting of six potential B cell epitopes and two potential T cell epitopes of FMDV, under the control of CMV promoter, was further inserted into the gE/gI locus of genome of TK(-)/gG(-)/P1(+), resulting in a new recombinant FHG/P1/PRV. The immunogenicity of FHG/P1/PRV was evaluated and compared with TK(-)/gG(-)/P1(+) in piglets. Our results clearly showed that FHG/P1/PRV performed better than or comparable with TK(-)/gG(-)/P1(+), as demonstrated by comparable PRV-specific neutralizing antibodies, enhanced FMDV-specific neutralizing antibodies, and cellular immune responses. More importantly, no gE- and gG-specific antibodies could be detected in pigs immunized with FHG/P1/PRV. These data indicate that FHG/P1/PRV is a promising bivalent vaccine candidate with more extensive potential application than TK(-)/gG(-)/P1(+) against both pseudorabies and FMD.


Assuntos
Proteínas do Capsídeo/imunologia , Vírus da Febre Aftosa/imunologia , Febre Aftosa/imunologia , Herpesvirus Suídeo 1/imunologia , Pseudorraiva/imunologia , Proteínas Virais/imunologia , Vacinas Virais/imunologia , Animais , Anticorpos Antivirais/sangue , Epitopos , Febre Aftosa/prevenção & controle , Febre Aftosa/virologia , Herpesvirus Suídeo 1/genética , Imunidade Celular , Plasmídeos , Pseudorraiva/prevenção & controle , Pseudorraiva/virologia , Suínos , Vacinas Sintéticas/imunologia , Proteínas Virais/genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA