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1.
Exp Biol Med (Maywood) ; : 15353702211038511, 2021 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-34514884

RESUMO

In breast cancer, tumor-associated macrophages with activated phenotypes promote tumor invasion and metastasis. The more aggressive mesenchymal-like breast cancer cells have a selective advantage, skewing macrophages toward the more immunosuppressive subtype. However, the mechanism underlying this shift is poorly understood. Cyclin D1b is a highly oncogenic variant of cyclin D1. Our previous study showed that non-metastatic epithelial-like breast cancer cells were highly metastatic in vivo when cyclin D1b was overexpressed. The present study determined whether cyclin D1b contributed to the interaction between breast cancer cells and macrophages. The results showed that cyclin D1b promoted the invasion of breast cancer cells in vitro. Specifically, through overexpression of cyclin D1b, breast cancer cells regulated the differentiation of macrophages into a more immunosuppressive M2 phenotype. Notably, tumor cells overexpressing cyclin D1b activated macrophages and induced migration of breast cancer cells. Further investigations indicated that SDF-1 mediated macrophage activation through breast cancer cells overexpressing cyclin D1b. These results revealed a previously unknown link between aggressive breast cancer cells and Tumor-associated macrophages, and highlighted the importance of cyclin D1b activity in the breast cancer microenvironment.

2.
J Int Med Res ; 49(7): 3000605211033495, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34292764

RESUMO

OBJECTIVE: To investigate the predictive value of hyperhomocysteinaemia (HHcy) for obstructive coronary artery disease (CAD) in an Asian population in northern China. METHODS: This retrospective study enrolled patients at their first cardiac assessment and assigned them to an obstructive CAD group or a non-obstructive CAD group according to the coronary angiography results. HHcy was defined as a homocysteine (Hcy) level > 15 µmol/l. RESULTS: This study enrolled 2987 participants: 1172 in the non-obstructive CAD group and 1815 in the obstructive CAD group. Hcy level in the obstructive CAD group was significantly higher than in the non-obstructive CAD group. The proportion of patients with HHcy in the obstructive CAD group was significantly greater than in the non-obstructive CAD group. Multivariate logistic regression analysis demonstrated that HHcy was independently correlated with obstructive CAD in both young (aged ≤ 55 years) and old patients (aged > 55 years). HHcy showed a higher sensitivity (93.1%), specificity (86.1%) and accuracy (90.0%) for obstructive CAD. The odds ratio for HHcy was 84.2. The Kappa value (0.8) showed substantial agreement between obstructive CAD and HHcy. CONCLUSIONS: HHcy was associated with obstructive CAD and may be a potentially independent risk factor for obstructive CAD with good predictive value.


Assuntos
Doença da Artéria Coronariana , Hiper-Homocisteinemia , China , Angiografia Coronária , Doença da Artéria Coronariana/diagnóstico por imagem , Homocisteína , Humanos , Hiper-Homocisteinemia/complicações , Hiper-Homocisteinemia/diagnóstico , Estudos Retrospectivos , Fatores de Risco
3.
Endocr J ; 68(9): 1109-1116, 2021 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-34121038

RESUMO

Gamma-glutamylcyclotransferase (GGCT) can promote the progression of osteosarcoma (OS). MicroRNAs also play significant roles in regulating the progression of OS. This study was designed to investigate whether miR-877 exerts its function in OS by targeting GGCT. The proliferation of OS cells (Saos-2 and U2OS) was detected by MTT and colony formation assays. The migration and invasion of OS cells were detected by transwell assays. The expressions of miRNAs and GGCT were detected by quantitative real-time PCR and Western blot. The luciferase reporter assay was performed to assess whether miR-877 could target GGCT. miR-877 was down-regulated both in OS tissues and OS cell lines (Saos-2 and U2OS). The overexpression of miR-877 inhibited the proliferation, migration, and invasion of OS cell lines, while the knockdown of miR-877 could negate effects. The expression of GGCT was increased in Saos-2 and U2OS cells. miR-877 could target GGCT, and the mRNA level of GGCT in Saos-2 and U2OS cells was decreased by the overexpression of miR-877. miR-877 overexpression inhibited the migration and invasion and suppressed the proliferation of Saos-2 and U2OS cells, and the overexpression of GGCT reversed this effects. The knockdown of miR-877 promoted the migration and invasion and facilitated the proliferation of Saos-2 and U2OS cells, and the silence of GGCT abolished this effects. Our findings suggested that miR-877 could inhibit the proliferation, migration, and invasion of OS cells by targeting GGCT.

4.
Carbohydr Polym ; 240: 116271, 2020 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-32475560

RESUMO

This study was designed to explore the effect of Chitosan oligosaccharides (COS) on mouse hindlimb ischemia by femoral artery ligation. Here, we demonstrated that COS treatment statistically promoted the blood perfusion and neovascularization in ischemic hindlimb of mice, accompanied by the suppression of inflammation and oxidative stress. By 16S rDNA gene sequencing, the disbalanced gut microbiota was observed in ischemic mice, while COS treatment, at least in part, restored the abundance changes of some intestinal bacteria at either phylum or genus levels. Based on metabolomics analysis on mouse plasma by UPLC-QTOF-MS, we screened 20 metabolites with the largest responses to ischemia, several of which were markedly reversed by COS. By Spearman's correlation analysis, the changed metabolites might act as a bridge between improved intestinal bacterial structure and alleviated hindlimb ischemia of mice treated by COS. Our studies point towards a potential role of COS in treatment of peripheral ischemia diseases.


Assuntos
Quitosana/uso terapêutico , Microbioma Gastrointestinal/efeitos dos fármacos , Membro Posterior/efeitos dos fármacos , Isquemia/tratamento farmacológico , Neovascularização Fisiológica/efeitos dos fármacos , Animais , Artéria Femoral/efeitos dos fármacos , Artéria Femoral/fisiologia , Membro Posterior/patologia , Inflamação/tratamento farmacológico , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Músculo Esquelético/irrigação sanguínea , Músculo Esquelético/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Fluxo Sanguíneo Regional
5.
Artigo em Inglês | MEDLINE | ID: mdl-32098225

RESUMO

Land consolidation is essential in China for improving land use efficiency and supporting rural public space governance. Previously, the implementation of land consolidation projects (LCPs) had been mainly led by governments in China. In recent years, the Chinese government vigorously promoted land consolidation, and land consolidation in the corporation-leading mode (CLM) has emerged. This study focused on investigating the performance of CLM projects and the difference in performance levels between the CLM projects and the government-leading mode (GLM) projects. Based on the improved extensible matter-element model, the performance levels of 14 LCPs in GLM and CLM of Jianghan Plain, Hubei Province and related impact factors were analyzed. A set of evaluation indices was selected based on the "process-based thinking and logic". Results showed that: (1) performance levels of the 14 LCPs are different, most of the projects in the GLM have ordinary or poor performance, while most of the projects in the CLM have excellent or good performance; (2) factors affecting the performance levels of LCPs are also different in the two modes. The main influencing factors in the CLM were the poor access to field roads, insufficient shelterbelt planting, and low land reclamation efficiency, while the insufficient shelterbelt planting, low annual output value of farmland and grain production capacity, low increase rate of agricultural labor production, and low land reclamation efficiency were the main influencing factors in the GLM; (3) comparative analysis of the two modes revealed that LCPs in the CLM have clear investment directions, high output benefits, and obvious advantages in the development of modern agriculture when compared with the GLM. To achieve improving the performance levels of LCPs, policy makers should actively innovate the implementation mode of LCPs and encourage all kinds of agricultural corporations to participate in land consolidation.


Assuntos
Agricultura , Conservação dos Recursos Naturais , Meio Ambiente , China , Fazendas
6.
Exp Ther Med ; 19(1): 519-526, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31885698

RESUMO

The present study aimed to retrospectively analyze the safety and efficacy of the early surgical management of thoracic tuberculosis (TB) in patients with neurological deficits. The medical data of patients with thoracic TB exhibiting neurological deficit in the Chest Hospital of Hebei Province were retrospectively reviewed. A total of 234 cases, including 123 males and 115 females, were recruited in the present study. Their pre- and postoperative neurological deficit and pain levels were assessed using the 2002 American spinal injury association (ASIA) impairment scale and visual analog scale, respectively. Patients were divided into two groups according to whether their preoperative standardized anti-TB treatment time was ≥4 weeks or <4 weeks. There was no difference in blood loss and operation time between the two groups. The erythrocyte sedimentation rate was higher in patients receiving standard anti-TB <4 weeks prior to and 1 month following surgery compared with the ≥4 weeks group, but the difference was not significant 6 months following surgery. ASIA scale scores all increased significantly 1 month following surgery in the <4 weeks group compared with the ≥4 weeks group (P=0.001) though there was no difference between the scores prior to surgery. ASIA scale scores improved to 4.4±0.5 and 4.5±0.4 in patients with anti-TB treatment times of ≥4 weeks and <4 weeks, respectively, 24 months following surgery (P=0.0895). The present study demonstrated that for patients with thoracic TB exhibiting neurological deficit, early surgical management following <4 weeks' standard anti-TB treatment is recommended. It may relieve spinal cord compression and also benefit the early recovery of neurological function in these patients.

7.
J Gastroenterol ; 55(2): 142-158, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31845054

RESUMO

Non-alcoholic fatty liver disease (NAFLD) is the hepatic manifestation of dysregulated lipid and glucose metabolism, which is often associated with obesity, dyslipidemia and insulin resistance. In view of the high morbidity and health risks of NAFLD, the lack of effective cure has drawn great attention. In recent years, a line of evidence has suggested a close linkage between the intestine and liver diseases such as NAFLD. We summarized the composition and characteristics of intestinal microbes and reviewed molecular insights into the intestinal microbiome in development and progression of NAFLD. Intestinal microbes mainly include bacteria, archaea, viruses and fungi, and the crosstalk between non-bacterial intestinal microbes and human liver diseases should be paid more attention. Intestinal microbiota imbalance may not only increase the intestinal permeability to gut microbes but also lead to liver exposure to harmful substances that promote hepatic lipogenesis and fibrosis. Furthermore, we focused on reviewing the latest "gut-liver axis"-targeting treatment, including the application of antibiotics, probiotics, prebiotics, synbiotics, farnesoid X receptor agonists, bile acid sequestrants, gut-derived hormones, adsorbents and fecal microbiota transplantation for NAFLD. In this review, we also discussed the potential mechanisms of "gut-liver axis" manipulation and efficacy of these therapeutic strategies for NAFLD treatment.


Assuntos
Disbiose/terapia , Microbioma Gastrointestinal , Hepatopatia Gordurosa não Alcoólica/microbiologia , Hepatopatia Gordurosa não Alcoólica/terapia , Receptores Citoplasmáticos e Nucleares/agonistas , Animais , Antibacterianos/uso terapêutico , Ácidos e Sais Biliares/metabolismo , Disbiose/complicações , Transplante de Microbiota Fecal , Humanos , Mucosa Intestinal/microbiologia , Mucosa Intestinal/fisiologia , Prebióticos , Probióticos/uso terapêutico , Receptores Citoplasmáticos e Nucleares/metabolismo , Transdução de Sinais , Simbióticos
8.
Langmuir ; 35(14): 5050-5053, 2019 04 09.
Artigo em Inglês | MEDLINE | ID: mdl-30879305

RESUMO

We report a triplex-based DNA device coupled with molybdenum disulfide (MoS2) nanosheets for use as a pH-sensing platform. The device transitions from a duplex state at pH 8 to a triplex state at pH 5. The interaction of the device with MoS2 nanosheets in the two states is read out as a fluorescence signal from a pH-insensitive dye attached to the device. We characterized the operation of the DNA device on MoS2 nanosheets, analyzed the pH response, and tested the reversibility of the system. Our strategy can lead to the creation of a suite of biosensors where the sensing element is a triplex DNA device and the signal response is modulated by inorganic nanomaterials.


Assuntos
DNA/química , Dissulfetos/química , Molibdênio/química , Nanoestruturas/química , Fluorescência , Concentração de Íons de Hidrogênio , Tamanho da Partícula , Propriedades de Superfície
9.
Curr Med Sci ; 38(3): 467-472, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30074214

RESUMO

Epithelial-to-mesenchymal transition (EMT) plays a critical role in cancer metastasis, and is relevant to the inflammatory microenvironment. Lipopolysaccharide (LPS), a cell wall constituent of gram-negative bacteria, has been reported to induce EMT of cancer cells through TLR4 signal. We previously reported that LPS promoted metastasis of mesenchymallike breast cancer cells with high expression of cyclin D1b. However, the role of cyclin D1b in LPS-induced EMT has not been fully elucidated. In the present study, we described that cyclin D1b augmented EMT induced by LPS in MCF-7 breast cancer cells. Cyclin D1b markedly amplified integrin αvß3 expression, which was further up-regulated under LPS stimulation. Our results showed ectopic expression of cyclin D1b promoted invasiveness of epithelial-like MCF-7 cells under LPS stimulation. Additionally, LPS-induced metastasis and EMT in MCF-7-D1b cells might depend on αvß3 expression. Further exploration indicated that cyclin D1b cooperated with HoxD3, a transcription factor promoting αvß3 expression, to promote LPSinduced EMT. Knockout of HoxD3 repressed LPS-induced EMT and αvß3 over-expression in MCF-7 cells with high expression of cyclin D1b. Specifically, all these effects were in a cyclin Dla independent manner. Taken all together, LPS up-regulated integrin αvß3 expression in MCF-7 cells with high expression of cyclin D1b and induced EMT in breast cancer cells, which highlights that cyclin D1b may act as an endogenous pathway participating in exogenous signal inducing EMT in breast cancer cells.


Assuntos
Processamento Alternativo/genética , Neoplasias da Mama/genética , Neoplasias da Mama/patologia , Ciclina D1/genética , Transição Epitelial-Mesenquimal/genética , Integrina alfaVbeta3/metabolismo , Lipopolissacarídeos/farmacologia , Processamento Alternativo/efeitos dos fármacos , Adesão Celular/efeitos dos fármacos , Movimento Celular/efeitos dos fármacos , Ciclina D1/metabolismo , Transição Epitelial-Mesenquimal/efeitos dos fármacos , Feminino , Fibrinogênio/farmacologia , Proteínas de Homeodomínio/metabolismo , Humanos , Células MCF-7 , Invasividade Neoplásica , Metástase Neoplásica , Fatores de Transcrição , Transfecção , Regulação para Cima/efeitos dos fármacos
10.
Acta Cardiol ; 73(1): 76-83, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28830299

RESUMO

OBJECTIVE: The management of patients with intermediate coronary lesions is a major clinical issue. Fractional flow reserve (FFR) is considered the gold criterion for the assessment of ischaemic stenosis, but it requires an invasive procedure. Coronary computed tomography angiography (CTA) for fractional flow reserve (FFRCT) is a novel noninvasive alternative for the diagnosis of ischaemic lesions. The aim was to determine the diagnostic efficacy of FFRCT for ischaemic coronary artery stenosis lesions of intermediate severity. METHODS: A total of 129 patients underwent 64-row dual-source CTA and invasive coronary angiography (ICA). In all, 156 vessels were identified as intermediate-grade coronary artery stenosis by subsequent ICA, defined as a maximum diameter reduction of 50%-70%. The FFR was also measured during ICA. FFRCT was computed from the three-dimensional dual-source CTA model and coronary flow dynamics data. RESULTS: Per-patient diagnostic sensitivity, specificity, positive predictive values, negative predictive values and accuracy of FFRCT amounted to 89.2%, 81.5%, 66.0%, 94.9% and 83.7%, respectively; and 86.9%, 73.6%, 58.0%, 93.1% and 77.6% on the per-vessel basis, respectively. FFRCT and FFR showed a good positive correlation. Bland-Altman analysis displayed good concordance between FFRCT and FFR. The receiver operating characteristic curve revealed that the area under the curve of FFRCT was 0.918 (95% confidence interval 0.849-0.986) on the per-patient analysis and 0.916 (95% confidence interval 0.863-0.969) on per-vessel analysis, respectively. CONCLUSIONS: FFRCT is featured by moderate accuracy in discriminating lesions of intermediate coronary artery stenosis that cause myocardial ischaemia. Impact statement How to treat intermediate coronary stenosis represents a major clinical issue. FFRCT has recently emerged as a novel noninvasive method evaluating ischemic lesions. In this study, we defined such lesion as 50-70% diameter stenosis. We designed the study to assess the diagnostic efficacy of FFRCT both at per-vessel level and at per-vessel levels for ischemic lesions.


Assuntos
Angiografia Coronária/métodos , Estenose Coronária/diagnóstico , Vasos Coronários/diagnóstico por imagem , Reserva Fracionada de Fluxo Miocárdico/fisiologia , Tomografia Computadorizada Multidetectores/métodos , Estenose Coronária/fisiopatologia , Vasos Coronários/fisiopatologia , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Curva ROC , Reprodutibilidade dos Testes , Estudos Retrospectivos , Índice de Gravidade de Doença
11.
Mol Med Rep ; 16(4): 4871-4878, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28791379

RESUMO

Oxidative stress and inflammation are involved in the pathogenesis of atherosclerosis. Calcium channel blockers (CCBs) inhibit the development of atherosclerosis, although the underlying molecular basis has not been completely elucidated. The present study was designed to investigate the effects of felodipine, a CCB, on inflammation and oxidative stress in human umbilical vein endothelial cells (HUVECs) and to examine the underlying mechanisms of action. Oxidized low­density lipoprotein (ox­LDL) was used to induce an inflammatory response in HUVECs. The effects of felodipine were investigated by measuring the content of nitric oxide (NO) and reactive oxygen species (ROS), the mRNA and protein levels of intercellular adhesion molecule 1 (ICAM­1) and vascular cell adhesion protein 1 (VCAM­1), and the mRNA levels of endothelial NO synthase (eNOS) and inducible NO synthase (iNOS), in addition to the adhesion ability of U937 cells to HUVECs. ROS and NO levels were significantly increased in HUVECs following 24­h treatment with 25 mg/l ox­LDL (P<0.01). The increase in ROS was reversed by treatment with felodipine. In addition, NO levels were increased following treatment with 1 µmol/l felodipine (P<0.05). The mRNA expression of ICAM­1, VCAM­1, eNOS and iNOS was increased (P<0.05). Administration of 0.1 µM felodipine significantly decreased the expression of ICAM­1, VCAM­1, and iNOS (P<0.05). The number of U937 cells adhered to ox­LDL­treated HUVECs was significantly increased compared with control, which was reversed by felodipine (0.1 µM). In conclusion, felodipine was demonstrated to inhibit oxidative stress and inflammatory responses, suggesting that it may be used to treat atherosclerosis.


Assuntos
Felodipino/farmacologia , Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos , Células Endoteliais da Veia Umbilical Humana/metabolismo , Inflamação/metabolismo , Lipoproteínas LDL/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Células Cultivadas , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Inflamação/tratamento farmacológico , Inflamação/genética , Molécula 1 de Adesão Intercelular/genética , Molécula 1 de Adesão Intercelular/metabolismo , Óxido Nítrico/metabolismo , Óxido Nítrico Sintase Tipo II/genética , Óxido Nítrico Sintase Tipo II/metabolismo , Óxido Nítrico Sintase Tipo III/genética , Óxido Nítrico Sintase Tipo III/metabolismo , Molécula 1 de Adesão de Célula Vascular/genética , Molécula 1 de Adesão de Célula Vascular/metabolismo
12.
Clin Cardiol ; 40(5): 307-313, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28026044

RESUMO

BACKGROUND: Immune response has been postulated to play a prominent role in the pathogenesis of peripartum cardiomyopathy (PPCM). Given the importance of programmed death (PD)-1 and its ligand B7 homologue 1 (B7-H1) costimulatory molecules as an immune regulatory pathway, this study aimed to investigate the effect of PD-1 and B7-H1 expression on immune response in peripheral blood lymphocytes from the patients with PPCM. HYPOTHESIS: PD-1 and B7-H1 may be involved in modulating immune response in PPCM. METHODS: Peripheral blood lymphocytes were obtained from PPCM and pregnancy-matched healthy women. PD-1 and B7-H1 expression were determined using fluorescence quantitative reverse transcription-polymerase chain reactions (RT-PCR) and Western blot. The presence of serum interferon (IFN)-γ and interleukin (IL)-4 were determined with enzyme-linked immunosorbent assay. RESULTS: The levels of pro-brain natriuretic peptide and IFN-γ were markedly elevated, whereas the levels of left ventricular ejection fraction and IL-4 were significantly reduced in PPCM patients compared to controls. Additionally, both RT-PCR and Western blot revealed that the levels of PD-1 and B7-H1 expression were decreased significantly in PPCM patients compared with controls. A significant positive correlation was observed between PD-1 and B7-H1 expression. Furthermore, PD-1 and B7-H1 expression showed significant negative correlation with IFN-γ, as well as positive correlation with IL-4. Therefore, decreased expression of PD-1 and B7-H1 led to a dysregulating immune response such that cellular immunity linked to T helper (Th)1 cells was predominant over humoral immunity linked to Th2 cells in PPCM. CONCLUSIONS: This study provided the first findings that PD-1 and B7-H1 expression were decreased, which might impair functional regulation of negative costimulation on immune response that may work in the etiopathogenesis of PPCM.


Assuntos
Antígeno B7-H1/sangue , Cardiomiopatias/sangue , Período Periparto/sangue , Complicações Cardiovasculares na Gravidez/sangue , Receptor de Morte Celular Programada 1/sangue , Células Th1/química , Adolescente , Adulto , Antígeno B7-H1/genética , Antígeno B7-H1/imunologia , Cardiomiopatias/diagnóstico , Cardiomiopatias/imunologia , Estudos de Casos e Controles , Feminino , Humanos , Imunidade Celular , Imunidade Humoral , Interferon gama/sangue , Interleucina-4/sangue , Peptídeo Natriurético Encefálico/sangue , Gravidez , Complicações Cardiovasculares na Gravidez/diagnóstico , Complicações Cardiovasculares na Gravidez/imunologia , Receptor de Morte Celular Programada 1/genética , Receptor de Morte Celular Programada 1/imunologia , Células Th1/imunologia , Células Th2/química , Células Th2/imunologia , Adulto Jovem
13.
Arch Med Sci ; 12(6): 1214-1219, 2016 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-27904510

RESUMO

INTRODUCTION: Angiopoietin-2 (Ang-2) plays a critical role in inducing tumor cell infiltration, and this invasive phenotype is caused by up-regulation of matrix metalloproteinase (MMP)-2. The relationship between Ang-2 and MMP-2 in atherosclerosis has not been reported yet. The aim is to measure the plasma concentrations of Ang-1, Ang-2 and MMP-2 and assess the correlation between the concentrations of these factors in coronary heart disease (CHD) patients. MATERIAL AND METHODS: The testing was done in a cross-sectional study. We prospectively enrolled 42 individuals with acute myocardial infarction, 42 individuals with unstable angina pectoris, 42 individuals with stable angina pectoris and 45 healthy control subjects. Concentrations of Ang-1, Ang-2 and MMP-2 were measured using the enzyme-linked immunosorbent assay (ELISA) method. Spearman's rank correlation was calculated to evaluate the relationships between MMP-2 and Ang-1, and MMP-2 and Ang-2 in patients with CHD. RESULTS: Patients with acute myocardial infarction and unstable angina pectoris had higher Ang-2 and MMP-2 levels compared with stable angina patients and healthy control subjects (p < 0.05), while concentrations of Ang-1 were not statistically different between the groups. Spearman's rank correlation showed that Ang-2 levels positively correlated with MMP-2 in patients with CHD (r = 0.679, p < 0.001). CONCLUSIONS: Plasma Ang-2 and MMP-2 levels but not Ang-1 levels were increased in patients with CHD. Ang-1 correlated weakly with MMP-2, whereas the Ang-2 and MMP-2 correlation was strong in patients with CHD. Ang-2 may play a role in atherosclerosis, and have an interaction with MMP-2.

14.
Microvasc Res ; 100: 17-24, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25937083

RESUMO

Serotonin (5-HT) has been shown to be involved in pulmonary vascular remodeling in pulmonary arterial hypertension (PAH) by inducing pulmonary artery smooth muscle cells (PASMCs) proliferation and inhibiting PASMC apoptosis. Peroxisome proliferator-activated receptor γ (PPARγ) plays a crucial role in regulating proliferation and apoptosis of many cell types. Moreover, recently, loss of PPARγ has also been reported to be associated with the development of PAH. The present study is aimed to assess whether PPARγ is involved in 5-HT induced PASMC proliferation and apoptosis inhibition and the possible mechanism. We found that 5-HT could induce PASMC proliferation and inhibit PASMC apoptosis in a dose-dependent manner. Furthermore, we found that 5-HT negatively regulated PPARγ expression and gene promoter activity in PASMCs and 5-HT induced PASMC proliferation and apoptosis resistance could be abolished by PPARγ agonists and enhanced by PPARγ inhibitor. In addition, we found that extracellular signal-regulated kinase (ERK) signaling pathway mediated the 5-HT-induced inhibition of PPARγ expression. Our results might provide novel insights into the mechanisms for the pro-remodeling action of 5-HT in pulmonary vasculature.


Assuntos
Apoptose/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Proteína Quinase 1 Ativada por Mitógeno/metabolismo , Proteína Quinase 3 Ativada por Mitógeno/metabolismo , Músculo Liso Vascular/efeitos dos fármacos , Miócitos de Músculo Liso/efeitos dos fármacos , PPAR gama/metabolismo , Serotonina/farmacologia , Transdução de Sinais/efeitos dos fármacos , Proteínas Reguladoras de Apoptose/metabolismo , Células Cultivadas , Relação Dose-Resposta a Droga , Humanos , Proteína Quinase 1 Ativada por Mitógeno/antagonistas & inibidores , Proteína Quinase 3 Ativada por Mitógeno/antagonistas & inibidores , Músculo Liso Vascular/enzimologia , Músculo Liso Vascular/patologia , Miócitos de Músculo Liso/enzimologia , Miócitos de Músculo Liso/patologia , PPAR gama/efeitos dos fármacos , PPAR gama/genética , Regiões Promotoras Genéticas , Inibidores de Proteínas Quinases/farmacologia , Artéria Pulmonar/efeitos dos fármacos , Artéria Pulmonar/enzimologia , Artéria Pulmonar/patologia , RNA Mensageiro/metabolismo
15.
Ying Yong Sheng Tai Xue Bao ; 25(3): 883-91, 2014 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-24984511

RESUMO

Based on the land use vector data of Wuhan Urban Agglomeration in the years 1990, 2000 and 2009, this paper used Costanza' s evaluation formula to estimate the ecosystem service value (ESV) of the study area according to "equivalent value per unit area of ecosystem services in China" and analyze its spatio-temporal characteristics. Then the correlation analysis was applied to explore the association between the ESV evolution and the land use changes. The results showed that due to the substantial growth of water area, the ESV of Wuhan Urban Agglomeration increased by 9.5% during the study period, which showed an overall rising trend. The ESV of water regulation and waste treatment increased obviously. Furthermore, the ESV changes showed obvious regional differences, which were most significant in Xiantao, Xinzhou and Yunmeng. The ESV was higher in the southeast and lower in the northwest. Over time, a Wuhan-centered "low-high-low" hierarchically distributed structure of ESV was formed in the eastern, western and northern parts. The ecologic dominance of the northern mountainous and hilly region was gradually abated, while a structural expansion with a high-ESV cluster had taken place in the southern part of the region in 2009. During the research period, the temporal change of ESV in Wuhan Urban Agglomeration was positively correlated with the area changes of forestland, water, grassland and cultivated land. However, the spatially balanced distribution of ESV was negatively correlated with the dispersion degrees of the cultivated land and unused land.


Assuntos
Ecologia/métodos , Ecossistema , China , Florestas , Pradaria , Modelos Teóricos , Análise Espaço-Temporal , Água
16.
Chin Med Sci J ; 20(1): 5-10, 2005 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-15844302

RESUMO

OBJECTIVE: To investigate whether intrapericardial urokinase irrigation along with pericardiocentesis could prevent pericardial constriction in patients with infectious exudative pericarditis. METHODS: A total of 94 patients diagnosed as infectious exudative pericarditis (34 patients with purulent pericarditis and 60 with tuberculous pericarditis, the disease courses of all patients were less than 1 month), 44 males and 50 females, aged from 9 to 66 years (mean 45.4 +/- 14.7 years), were consecutively recruited from 1993 to 2002. All individuals were randomly given either intrapericardial urokinase along with conventional treatment in study group, or conventional treatment alone (including pericardiocentesis and drainage) in control group. The dosage of urokinase ranged from 200000 to 600000 U (mean 320000 +/- 70000 U). The immediate effects were detected by pericardiography with sterilized air and diatrizoate meglumine as contrast media. The long-term investigation depended on the telephonic survey and echocardiographic examination. The duration of following-up ranged from 8 to 120 months (mean 56.8 +/- 29.0 months). RESULTS: Percutaneous intrapericardial urokinase irrigation promoted complete drainage of pericardial effusion, significantly reduced the thickness of pericardium (from 3.1 +/- 1.6 mm to 1.6 +/- 1.0 mm in study group, P < 0.001; from 3.4 +/- 1.6 mm to 3.2 +/- 1.8 mm in control group, P > 0.05, respectively), and alleviated the adhesion. Intrapericardial bleeding related to fibrinolysis was found in 6 of 47 patients with non-blood pericardial effusion and no systemic bleeding and severe puncture-related complication was observed. In follow-up, there was no cardiac death, and pericardial constriction events were observed in 9 (19.1%) of study group and 27 (57.4%) of control group. Cox analysis illustrated that urokinase could significantly reduce the occurrence of pericardial constriction (relative hazard coefficient = 0.185, P < 0.0001). CONCLUSION: The early employment of intrapericardial fibrinolysis with urokinase and pericardiocentesis appears to be safe and effective in preventing the development of pericardial constriction in patients with infectious exudative pericarditis.


Assuntos
Fibrinolíticos/administração & dosagem , Pericardite Constritiva/prevenção & controle , Pericardite/tratamento farmacológico , Terapia Trombolítica , Ativador de Plasminogênio Tipo Uroquinase/administração & dosagem , Adolescente , Adulto , Idoso , Criança , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Pericardiocentese , Pericardite/terapia
17.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 35(5): 719-22, 2004 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-15460429

RESUMO

OBJECTIVE: Simultaneous recording of transmembrane action potential at endocardium, midcardium and epicardium and transmural ECG in arterially perfused left ventricular preparation is a new method for researching into the mechanism about ventricular arrhythmia, and in this connection, how to distinguish the perfused area plays a key role in keeping preparations under normal condition. This study is aimed to evaluate the effects of Evan Blue on the displaying of the perfused area and on the characters of transmembrane action potential of the arterially perfused left ventricular preparations. METHODS: Rabbit left ventricular wedge preparations were perfused with Tyrode solution continuously via left circumflex, and the action potential of endocardium, midmyocardium, epicardium or transmural electrocardiogram were recorded simultaneously. The action poatential duration (APD), transmural dispersion of repolarization (TDR) or QT intervals were compared and the color variation of the preparations were studied before and 30 min after perfusion with Evan Blue. RESULTS: Under the basic stimulatory cycle length of 1000, 2000, 4000 ms, there was no significant difference of APD in the same transmural layer or TDR before and after Evan Blue perfusion (P<0.01), but APD or TDR stimulated at basic cycle length of 1000-4000 ms were all higher than those recorded at 500 ms (P<0.01); APDs of endocardium were much longer than those of epicardium or midmyocardium (P<0.01); there was no significant difference in APD, TDR and QT intervals before and after Evan Blue perfusion (P>0.05). No premature ventricular contractions and ventricular tachycardia happened during the experiments. CONCLUSION: Evan Blue can be used as a marker to identify the perfused area.


Assuntos
Azul Evans/farmacologia , Função Ventricular Esquerda/fisiologia , Potenciais de Ação/fisiologia , Animais , Eletrocardiografia , Feminino , Técnicas In Vitro , Masculino , Perfusão , Coelhos
18.
Sheng Li Xue Bao ; 56(4): 487-92, 2004 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-15322684

RESUMO

Experiments were performed to investigate the effects of long-term treatment with adrenergic receptor antagonist on electrical remodeling of the left ventricle with chronic pressure-overload. New Zealand rabbits underwent subtotal banding of superrenal abdominal aorta. At 10 weeks after surgery, echocardiography examination was performed, then action potential (AP), inward rectifier potassium current (I(Ki)), delayed rectifier potassium current (I(K)) and Na(+)/Ca(2+) exchanger current (I(Na(+)/Ca(2+))) were recorded in midmyocardial cells isolated from left ventricle of abdominal aorta banded group (banded group), abdominal aorta banding plus Carvedilol intervention group (Carvedilol group), and normal control group rabbits by using the whole-cell patch-clamp techniques. The results showed that left ventricular mass index in control, banded, and Carvedilol groups were 1.78+/-0.06 (n=7), 2.33+/-0.11 (n=7), and 1.87+/-0.08 (n=7), respectively (banded vs control and Carvedilol, P<0.01). At basic cycle length of 2 s, AP duration (measured at 90% repolarization, APD(90), ms) in control, banded, and Carvedilol groups were 522.0+/-19.5 (n=6), 664.7+/-46.2 (n=7), 567.8+/-14.3 (n=8) respectively (banded vs control, P<0.01; Carvedilol vs banded, P<0.05). At test potential of -100 mV, inward I(Ki) density (pA/pF) in control, banded, and Carvedilol groups were -11.8+/-0.50 (n=8), -8.07+/-0.28 (n=8), -10.69+/-0.35 (n=8) respectively (banded vs control and Carvedilol, P<0.01). At test potential of +50 mV, I(K) tail current density (pA/pF) in control, banded, and Carvedilol groups were 0.59+/-0.04 (n=8), 0.40+/-0.02 (n=9), 0.51+/-0.02 (n=8) respectively (banded vs control, P<0.01; Carvedilol vs banded, P<0.05). At test potential of +60 mV, outward I(Na(+)/Ca(2+)) density (pA/pF) in control, banded, and Carvedilol groups were 1.06+/-0.11 (n=8), 1.54+/-0.10 (n=9), 1.24+/-0.07 (n=8), respectively (banded vs control and Carvedilol, P<0.01). At test potential of -120 mV, inward I(Na(+)/Ca(2+)) density (pA/pF) in control, banded, and Carvedilol groups were -0.54+/-0.06 (n =8), -0.75+/-0.04 (n=9), -0.60+/-0.03 (n=8), respectively (banded vs control, P<0.01; Carvedilol vs banded, P<0.05). It is shown that long-term treatment with Carvedilol not only prevents development of cardiac hypertrophy, but also improves the electrophysiological alterations in rabbit hearts with chronic pressure-overload. This finding may add new electrophysiological evidence for the treatment of heart failure and hypertension with adrenergic receptor antagonist.


Assuntos
Antagonistas Adrenérgicos/farmacologia , Carbazóis/farmacologia , Baixo Débito Cardíaco/fisiopatologia , Propanolaminas/farmacologia , Remodelação Ventricular/efeitos dos fármacos , Potenciais de Ação , Animais , Carvedilol , Eletrofisiologia , Feminino , Masculino , Técnicas de Patch-Clamp , Coelhos
19.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 35(4): 496-9, 2004 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-15291109

RESUMO

OBJECTIVE: To investigate the distribution characteristics of Na+/Ca2+ exchanger current (I(Na+/Ca2+)) across the left ventricular wall of rabbit and the relationship between I(Na+/Ca2+) and transmural depolarization heterogeneity. METHODS: By using whole-cell patch clamp techniques, action potentials (AP), I(Na+/Ca2+), and both rapid and slow components of delayed rectifier potassium current (I(Kr) and I(Ka)) were recorded in subendocardial (Endo), midmyocardial (M), and subepicardial (Epi) cells of the left ventricular wall of rabbit. RESULTS: AP duration in M cells was longer than that in Epi cells, P<0.01. At the test potential of +40 mV, outward I(Na+/Ca2+) in M cells was larger than that in Epi and Endo cells, P<0.01, P<0.05, respectively. At the test potential of -100 mV, inward I(Na+/Ca2+) in M cells was larger than that in Epi cells, P<0.05. At the test potential of +50 mV, the tail current density of I(Ka) in M cells was smaller than that in Epi cells, P<0.05, and there was no significant difference among the tail current densities of I(Kr) in Endo, M, and Epi cells. CONCLUSION: The distribution of I(Na+/Ca2+) and I(Ka) across the left ventricular wall of rabbit is unequal, which contributes to the transmural depolarization heterogeneity.


Assuntos
Miocárdio/metabolismo , Trocador de Sódio e Cálcio/metabolismo , Função Ventricular , Potenciais de Ação , Animais , Cálcio/metabolismo , Eletrofisiologia , Miócitos Cardíacos/metabolismo , Miócitos Cardíacos/fisiologia , Técnicas de Patch-Clamp , Coelhos , Sódio/metabolismo
20.
Di Yi Jun Yi Da Xue Xue Bao ; 24(4): 430-3, 436, 2004 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-15090316

RESUMO

OBJECTIVE: To investigate the characteristics of Na+/Ca2+ exchanger current (INa+ /Ca2+) and K+ current remodeling in midmyocardial cells of hypertrophic left ventricle for understanding the ionic basis of arrhythmia of the hypertrophic heart. METHODS: Twenty New Zealand rabbits were divided equally into normal control group and operation group, and in the latter, left ventricular hypertrophy was induced in the rabbits by partial ligation of the abdominal aorta. Action potentials, INa+/Ca2+, slowly activating delayed rectifier K+ current (IKs) and rapidly activating delayed rectifier K+ current (IKr) were recorded in the two groups by using whole-cell patch-clamp technique. RESULTS: At the basic cycle length of 2 s, 90% action potential duration (APD90) in control and operation groups was 522.0+/-19.5 ms (n=6) and 664.7+/-32.7 ms (n=7) respectively; at the testing potential of +40 mV, outward INa+/Ca2+ density in the two groups was 0.94+/-0.11 pA/pF (n=9) and 1.30+/-0.11 pA/pF (n=8) respectively; the testing potential of -100 mV elicited inward INa+/Ca2+ density of 0.40+/-0.05 pA/pF (n=9) and 0.56+/-0.02 pA/pF (n=8) respectively. The testing potential of +50 mV induced IKs tail current density of 0.26+/-0.03 pA/pF (n=8) and 0.17+/-0.01 pA/pF (n=9), and IKr tail current density of 0.34+/-0.02 pA/pF (n=8) and 0.23+/-0.02 pA/pF (n=9) respectively. Statistically significant differences were identified between the control and operation groups in all the above indices measured. CONCLUSION: The characteristics of electrical remodeling changes in midmyocardial cells of hypertrophic left ventricle, exhibited by prolonged action potential, up-regulated INa+/Ca2+ and down-regulated IKs and IKr.


Assuntos
Cálcio/metabolismo , Hipertrofia Ventricular Esquerda/metabolismo , Miocárdio/metabolismo , Canais de Potássio/fisiologia , Trocador de Sódio e Cálcio/fisiologia , Sódio/metabolismo , Potenciais de Ação , Animais , Ecocardiografia , Feminino , Hipertrofia Ventricular Esquerda/diagnóstico por imagem , Masculino , Coelhos
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