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1.
Environ Res ; 185: 109403, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32240842

RESUMO

An electrostimulated anaerobic-oxic integrated system was constructed for treating alizarin yellow R (AYR) containing wastewater. In electro-stimulated anaerobic unit, AYR decolorization efficiency improved from 51.2% to 96.6%. Two amination metabolites, p-phenylenediamine and 5-aminosalicylic acid, went through oxidation, ammonification and mineralization in oxic unit. Electro-stimulation promoted denitrification and COD removal efficiencies by 15.5% and 8.6%, respectively. A 20% improved nitrification efficiency was observed in oxic unit, due to elimination of AYR toxicity inhibition. No corrosion of heat-treated stainless steel occurred during the 60 days of continuous operation. Electrons sunk in denitrification and decolorization accounted for 34.4-36.8% of those released from COD removal, and 7.3% increase of removed nitrogen in nitrogenous compounds (AYR, nitrate and ammonia) was found. Electro-stimulated anaerobic unit predominated with fermentation and denitrification genera (Propionispira, Rhodocyclus, etc.) and aboundance of electro-active decolorization genus (Desulfovibrio, etc.) increased. Ammonia-oxidizing genus, Comamonas, was the most abundant in aerobic unit. Compared to the suspension, the electrostimulation could increased the abundance of electro-active genera in cathodic biofilm. This study revealed the feasibility of applying electro-stimulation and the conversion laws of nitrogenous organics in secondary bio-treatment system for treating toxic nitrogenous organics-contained wastewater.

2.
World J Surg ; 2020 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-32274535

RESUMO

BACKGROUND: This study aimed to investigate the transcatheter arterial embolization (TAE) in treatment of abdominal bleeding in patients being treated with open abdomen due to duodenal fistula. METHODS: This was a retrospective study performed at our center. From January 2005 to November 2010, all patients with abdominal bleeding were treated with surgical hemostasis (SH) and included in SH group. From January 2012 to December 2018, all patients with a bleeding were treated with TAE and included in the TAE group. Clinical data were reviewed and compared between the two groups. The effect of TAE in the management of abdominal bleeding was evaluated. RESULTS: A total of 131 patients were enrolled, and there were 64 in the SH group and 67 in the TAE group. The success rate of hemostasis was higher in the TAE group (89.55% vs. 73.44%, adjusted OR = 4.065, 95% CI 1.336-12.336, P = 0.013). Moreover, the recognition rate of hemorrhagic vessels in the TAE group was higher (91.04 vs. 51.56; P < 0.001). The re-bleeding occurred in 20 patients, 7(11.67%) in the TAE group and 13(27.66%) in the SH group. The re-bleeding rate in SH group was higher (adjusted HR = 2.564, 95% CI 1.023-6.428, P = 0.045) CONCLUSIONS: TAE is an effective method in treatment of abdominal bleeding in patients being treated with open abdomen due to duodenal fistula.

3.
J Invest Surg ; : 1-7, 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31795782

RESUMO

Background: This study aimed to investigate the effect of double-lumen irrigation-suction tube (DLIST) in the management of surgical site infections (SSIs) after enterocutaneous fistula (ECF) excisions. Method: From January 2016 to December 2017 medical records of patients with ECF excisions were reviewed. Patients with primary superficial SSI were divided into group a (treated with DLIST) and b (treated with delayed primary closures). Patients with primary deep SSI were divided into group A (treated with DLIST) and B (treated with vacuum-assisted closure [VAC]). The effect of the DLIST was evaluated. Results: There were 32 in group a and 27 in group b. The therapeutic time and cost in group a were lower (13.13 ± 2.37 d vs. 24.89 ± 7.44 d; p < .001; $1456 ± 302 vs.$2784 ± 583; p < .001). There were 21 in group A and 23 in group B. While the therapeutic time of group A was longer, the cost was lower ($1717 ± 404 vs. $2636 ± 592; p < .001). Conclusions: Placing DLIST is an effective and cheap method to treat superficial SSI after ECF excisions. The cost of DLIST in treatment of deep SSI is lower, while the effect of VAC is better.

4.
Int J Nanomedicine ; 14: 8059-8072, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31632018

RESUMO

Background: Compared with random copolymers, block copolymerization is easier to prepare for nanoparticles with core-shell structure, and they will have better glucose sensitivity and higher insulin loading. Purpose: In our study, insulin-loaded poly (3-acrylamidophenylboronic acid-block-N-vinyl caprolactam) p(AAPBA-b-NVCL) nanoparticles were successfully prepared and were glucose-sensitive, which could effectively lower the blood sugar levels within 72 hrs. Methods: The polymer of p(AAPBA-b-NVCL) was produced by reversible addition-fragmentation chain transfer polymerization based on different ratios of 3-acrylamidophenylboronic acid (AAPBA) and N-vinylcaprolactam (NVCL), and its structure was discussed by Fourier transform infrared spectroscopy and 1H-nuclear magnetic resonance . Next, the polymer was manufactured into the nanoparticles, and the characteristics of nanoparticles were detected by dynamic light scattering, lower critical solution temperature, and transmission electron microscopy. After that, the cell and animal toxicity of nanoparticles were also investigated. Results: The results demonstrated that p(AAPBA-b-NVCL) was successfully synthesized, and can be easily self-assembled to form nanoparticles. The new nanoparticles included monodisperse submicron particles, with the size of the nanoparticle ranged between 150 and 300nm and are glucose- and temperature-sensitive. Meanwhile, insulin can be easily loaded by p(AAPBA-b-NVCL) nanoparticles and an effective sustained release of insulin was observed when the nanoparticles were placed in physiological saline. Besides, MTT assay revealed that cell viability was more than 80%, and mice demonstrated no negative impact on blood biochemistry and heart, liver, spleen, lung, and kidney after intraperitoneal injection of 10 mg/kg/d of nanoparticles. This suggested that the nanoparticles were low-toxic to both cells and animals. Moreover, they could lower the blood sugar level within 72h. Conclusion: Our research suggested that these p(AAPBA-b-NVCL) nanoparticles might have the potential to be applied in a delivery system for insulin or other hypoglycemic proteins.


Assuntos
Acrilamidas/química , Ácidos Borônicos/química , Caprolactama/química , Sistemas de Liberação de Medicamentos , Glucose/análise , Insulina/administração & dosagem , Nanopartículas/química , Acrilamidas/síntese química , Animais , Glicemia/metabolismo , Ácidos Borônicos/síntese química , Caprolactama/análogos & derivados , Caprolactama/síntese química , Sobrevivência Celular/efeitos dos fármacos , Feminino , Hidrodinâmica , Concentração de Íons de Hidrogênio , Hipoglicemiantes/administração & dosagem , Hipoglicemiantes/farmacologia , Masculino , Camundongos , Células NIH 3T3 , Nanopartículas/ultraestrutura , Espectroscopia de Prótons por Ressonância Magnética , Espectroscopia de Infravermelho com Transformada de Fourier , Eletricidade Estática , Temperatura
5.
Artigo em Inglês | MEDLINE | ID: mdl-31448374

RESUMO

People with health concerns go to online health support groups to obtain help and advice. To do so, they frequently disclose personal details, many times in public. Although research in non-health settings suggests that people self-disclose less in public than in private, this pattern may not apply to health support groups where people want to get relevant help. Our work examines how the use of private and public channels influences members' self-disclosure in an online cancer support group, and how channels moderate the influence of self-disclosure on reciprocity and receiving support. By automatically measuring people's self-disclosure at scale, we found that members of cancer support groups revealed more negative self-disclosure in the public channels compared to the private channels. Although one's self-disclosure leads others to self-disclose and to provide support, these effects were generally stronger in the private channel. These channel effects probably occur because the public channels are the primary venue for support exchange, while the private channels are mainly used for follow-up conversations. We discuss theoretical and practical implications of our work.

6.
Medicine (Baltimore) ; 98(35): e16617, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31464898

RESUMO

It is unclear whether strategies targeting negative fluid balance are associated with facilitated early fascial closure. The present study investigated the effects of fluid removal therapy on early facial closure of open abdomen patients.A prospective study was conducted in patients who underwent open abdomen management with vacuum-assisted and mesh-mediated fascial traction technique. Therapeutic diuresis with torasemide was applied to cause negative fluid balance in the treatment group. The study and follow-up periods were 7 and 180 days, respectively. The observational indices included the intra-abdominal pressure, the number of days to closure, the type of closure, the septic complications, the duration of ventilation support, the duration of initial hospital stay, and the duration of intensive care unit (ICU) stay.A total of 27 patients were divided into the treatment (16 patients) and control (11 patients) groups. The median intra-abdominal pressure (IAP) of the patients of the control and the treatment groups was significantly lower at day 7 compared with the baseline value (P < .0001). IAP was lower in the treatment group compared with that noted in the control group, following day 4 of the fluid removal therapy (P < .05). The percentage weight loss in the treatment group was between 4.80% and 10.88%. The early closure rates were significantly higher in the treatment group compared with those in the control group (75.0% vs 18.2%, P = .0063).Fluid removal therapy combined with vacuum-assisted and mesh-mediated fascial traction provided a high early fascial closure rate for open abdomen patients.


Assuntos
Abdome/cirurgia , Técnicas de Fechamento de Ferimentos Abdominais/instrumentação , Diuréticos/administração & dosagem , Tratamento de Ferimentos com Pressão Negativa/instrumentação , Torasemida/administração & dosagem , Cicatrização , Adulto , Feminino , Humanos , Tempo de Internação , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Telas Cirúrgicas , Resultado do Tratamento
7.
Analyst ; 144(11): 3685-3690, 2019 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-31086899

RESUMO

Plant cell walls (CWs) with complex macromolecular structures can surround and protect cells from a variety of harsh environmental conditions such as pathogens, herbivores, and trace metals. Here, a novel strategy for in situ imaging of plant cell walls was developed to evaluate heavy metal pollution via thiolated full-color emissive carbon-dots (F-CDs) targeting Pb(ii)-adsorbed onion cell walls. The thiolated F-CDs with excellent optical properties from red light to blue light were synthesized through a facile electrochemical approach using new precursors of luminol and l-tryptophan and further modified with l-cysteine. Based on a strong covalent interaction of Pb(ii) and thiolated F-CDs, we achieved in situ fluorescence imaging for the Pb(ii) adsorbed on CWs, which showed enhanced red, blue and green multi-color fluorescence (FL) on CWs with increased Pb(ii)-ion content. In contrast, multi-color fluorescence on cytoplasm diminished, attributed to F-CDs targeting and accumulating on the cytoskeleton which thus limited F-CD diffusion into protoplasm. Therefore, in situ fluorescent images for CWs can demonstrate heavy metal contamination degrees in plant cells. This facile and undamaging protocol will be beneficial for investigating heavy metal migration into the protoplast and fast evaluation of food quality and safety.


Assuntos
Carbono/química , Parede Celular/química , Corantes Fluorescentes/química , Chumbo/análise , Pontos Quânticos/química , Adsorção , Cor , Colorimetria/métodos , Células Endoteliais/química , Fluorescência , Contaminação de Alimentos/análise , Química Verde/métodos , Chumbo/química , Cebolas/química
8.
Med Sci Monit ; 25: 2591-2598, 2019 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-30964125

RESUMO

BACKGROUND Prevalence and associated risk factors for pressure ulcers (PU) vary in different body areas and diseases. Few studies have focused on PU in patients with enterocutaneous fistula (ECF). The aim of the present study was to investigate the prevalence and risk factors for PU in patients with ECF. MATERIAL AND METHODS From January 2016 to June 2016, medical records of 140 patients with ECF who were transferred to the Enterocutaneous Fistula Treatment Center, Jinling Hospital, were reviewed and analyzed. The prevalence of PU was investigated. To evaluate the risk factors for PU in patients with ECF, 5 patients with PU before admission were excluded, and the remaining 135 patients were divided into 2 groups: the PU group and the non-PU group. The risk factors for PU were confirmed by multivariate logistic regression analysis of characteristics on admission. RESULTS There were 42 cases with PU (5 cases with PU before admission, 37 cases with PU in the treatment after admission), and the prevalence of PU in patients with ECF was 30%. In addition, Braden risk score <19 (OR=9.33, CI: 2.80-31.08, p<0.001); underweight (BMI<18.5) (OR=5.21, CI: 1.65-16.39, p=0.005); onset of duodenal fistula (OR=4.86, CI: 1.33-17.78, p=0.017); diabetes (OR=4.95, CI: 1.03-23.85, p=0.046); and APACHE II score (OR=1.34, CI: 1.04-1.72, p=0.019) were associated with PU. CONCLUSIONS The PU prevalence was 30% in patients with ECF. Braden risk score <19, underweight, onset of duodenal fistula, diabetes, and APACHE II score were risk factors for PU in patients with ECF.


Assuntos
Fístula Intestinal/complicações , Fístula Intestinal/fisiopatologia , Lesão por Pressão/etiologia , Adolescente , Adulto , Idoso , Grupo com Ancestrais do Continente Asiático/genética , China , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Estudos Retrospectivos , Fatores de Risco
9.
Sensors (Basel) ; 19(7)2019 Apr 02.
Artigo em Inglês | MEDLINE | ID: mdl-30987038

RESUMO

Multi-agent hybrid social cognitive optimization (MAHSCO) based on the Internet of Things (IoT) is suggested to solve the problem of the generation of formations of unmanned vehicles. Through the analysis of the unmanned vehicle formation problem, formation principles, formation scale, unmanned vehicle formation safety distance, and formation evaluation indicators are taken into consideration. The application of the IoT enables the optimization of distributed computing. To ensure the reliability of the formation algorithm, the convergence of MAHSCO has been proved. Finally, computer simulation and actual unmanned aerial vehicle (UAV) formation generation flight generating four typical formations are carried out. The result of the actual UAV formation generation flight is consistent with the simulation experiment, and the algorithm performs well. The MAHSCO algorithm based on the IoT is proved to be able to generate formations that meet the mission requirements quickly and accurately.


Assuntos
Aeronaves , Condução de Veículo , Robótica/tendências , Algoritmos , Simulação por Computador , Humanos , Internet , Veículos Automotores , Mídias Sociais
10.
Int J Mol Sci ; 20(2)2019 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-30654456

RESUMO

Cyclophilins (CYPs) are a member of the immunophilin superfamily (in addition to FKBPs and parvulins) and play a significant role in peptidyl-prolyl cis-trans isomerase (PPIase) activity. Previous studies have shown that CYPs have important functions in plants, but no genome-wide analysis of the cotton CYP gene family has been reported, and the specific biological function of this gene is still elusive. Based on the release of the cotton genome sequence, we identified 75, 78, 40 and 38 CYP gene sequences from G. barbadense, G. hirsutum, G. arboreum, and G. raimondii, respectively; 221 CYP genes were unequally located on chromosomes. Phylogenetic analysis showed that 231 CYP genes clustered into three major groups and eight subgroups. Collinearity analysis showed that segmental duplications played a significant role in the expansion of CYP members in cotton. There were light-responsiveness, abiotic-stress and hormone-response elements upstream of most of the CYPs. In addition, the motif composition analysis revealed that 49 cyclophilin proteins had extra domains, including TPR (tetratricopeptide repeat), coiled coil, U-box, RRM (RNA recognition motif), WD40 (RNA recognition motif) and zinc finger domains, along with the cyclophilin-like domain (CLD). The expression patterns based on qRT-PCR showed that six CYP expression levels showed greater differences between Xinhai21 (long fibres, G. barbadense) and Ashmon (short fibres, G. barbadense) at 10 and 20 days postanthesis (DPA). These results signified that CYP genes are involved in the elongation stage of cotton fibre development. This study provides a valuable resource for further investigations of CYP gene functions and molecular mechanisms in cotton.


Assuntos
Fibra de Algodão , Ciclofilinas/genética , Regulação da Expressão Gênica de Plantas , Genoma de Planta , Gossypium/crescimento & desenvolvimento , Gossypium/genética , Família Multigênica , Proteínas de Plantas/genética , Cromossomos de Plantas/genética , Genes de Plantas , Filogenia , Proteínas de Plantas/metabolismo , Regiões Promotoras Genéticas/genética , Especificidade da Espécie
11.
Eur J Med Chem ; 162: 348-363, 2019 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-30448420

RESUMO

Accetohydroxyacid synthase (AHAS) is the first enzyme involved in the biosynthetic pathway of branched-chain amino acids. Earlier gene mutation of Candida albicans in a mouse model suggested that this enzyme is a promising target of antifungals. Recent studies have demonstrated that some commercial AHAS-inhibiting sulfonylurea herbicides exerted desirable antifungal activity. In this study, we have designed and synthesized 68 novel ethoxysulfulron (ES) derivatives and evaluated their inhibition constants (Ki) against C. albicans AHAS and cell based minimum inhibitory concentration (MIC) values. The target compounds 5-1, 5-10, 5-22, 5-31 and 5-37 displayed stronger AHAS inhibitions than ES did. Compound 5-1 had the best Ki of 6.7 nM against fungal AHAS and MIC values of 2.5 mg/L against Candida albicans and Candica parapsilosis after 72 h. A suitable nematode model was established here and the antifungal activity of 5-1 was further evaluated in vivo. A possible binding mode was simulated via molecular docking and a comparative field analysis (CoMFA) model was constructed to understand the structure-activity relationship. The current study has indicated that some ES derivatives should be considered as promising hits to develop antifungal drugs with novel biological target.


Assuntos
Acetolactato Sintase/antagonistas & inibidores , Antifúngicos/química , Relação Quantitativa Estrutura-Atividade , Compostos de Sulfonilureia/farmacologia , Animais , Antifúngicos/farmacologia , Candida albicans/efeitos dos fármacos , Inibidores Enzimáticos/farmacologia , Herbicidas , Camundongos , Simulação de Acoplamento Molecular , Nematoides/efeitos dos fármacos , Compostos de Sulfonilureia/química
12.
Med Sci Monit ; 24: 9317-9323, 2018 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-30577040

RESUMO

BACKGROUND Abdominal bleeding is a severe complication of duodenal fistula, but few studies have focused on this problem. The purpose of the present study was to investigate the prevalence of and risk factors for intra-abdominal bleeding in patients with external duodenal fistula. MATERIAL AND METHODS From January 2014 to December 2016, medical records of 97 patients with external duodenal fistula were retrospectively reviewed and analyzed. The prevalence and risk factors for intra-abdominal bleeding were evaluated. RESULTS The prevalence of abdominal bleeding in patients with external duodenal fistula was 31.9% (95%CI: 22.5-41.4%). A total of 31 patients had intra-abdominal bleeding. Results revealed that acute kidney failure (OR: 8.462, 95% CI: 1.921-37.28, p=0.005) and retroperitoneal infection (OR: 5.373, 95% CI: 1.504-19.197, p=0.010) were associated with abdominal bleeding. CONCLUSIONS The prevalence of abdominal bleeding in patients with external duodenal fistula was 31.9%, and acute kidney failure and retroperitoneal infection were found to be risk factors for intra-abdominal bleeding.


Assuntos
Hemorragia/etiologia , Fístula Intestinal/complicações , Fístula Intestinal/etiologia , Abdome , Traumatismos Abdominais/etiologia , Lesão Renal Aguda , Adulto , Idoso , Fístula Cutânea/complicações , Fístula Cutânea/etiologia , Duodeno/fisiopatologia , Feminino , Humanos , Fístula Intestinal/sangue , Masculino , Metrorragia , Pessoa de Meia-Idade , Prevalência , Estudos Retrospectivos , Fatores de Risco
13.
Nanoscale ; 10(39): 18597-18605, 2018 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-30259937

RESUMO

A single-cell sensor with a spatial architecture was firstly fabricated for realizing high precision single-cell analysis using an 11-mercaptoundecanoic acid (MUA)-spaced sensing interface to prop up single cells and provide a suitable space for effective nanoprobe labeling. Mercapto acids (MA) with different carbon chain lengths were optimized and MUA was selected to provide optimal interspace on the electrodeposited PANI/AuNP substrates, and its carboxyl could couple with folic acid to capture cancer cells. Bifunctional Au@Cu-PbCQD nanoprobes, in which the AuNP cores were linked with lead-coadsorbed carbon quantum dots (PbCQDs) by a copper(ii) ion bridge, were firstly synthesized and applied as highly sensitive electrochemiluminescence (ECL) probes and electrochemical probes. Hyaluronic acid (HA)-functionalized Au@Cu-PbCQD nanoprobes were labelled on MCF-7 cells via specific recognition to the CD44 receptor, which served as the research model. The ECL response of the sensor was applied to evaluate the validity of nanoprobe labeling. With MUA modified, the sensor was able to enhance the ECL intensity by 37.5 ± 3.9%, indicating the remarkable amelioration of the accuracy of single-cell analysis. To take advantage of the bifunctional nanoprobes, differential pulse voltammetry (DPV) was further applied to confirm the feasibility of the proposed single-cell sensor with a spatial architecture. Therefore, the novel strategy provides a single-cell analysis platform to acquire high-precision analytical results, and more accurately to elucidate cellular heterogeneity and biological function.


Assuntos
Cobre/química , Técnicas Eletroquímicas , Ácidos Graxos/química , Ouro/química , Receptores de Hialuronatos/análise , Chumbo/química , Nanopartículas Metálicas/química , Sondas Moleculares/química , Compostos de Sulfidrila/química , Humanos , Receptores de Hialuronatos/química , Células MCF-7
14.
Sensors (Basel) ; 18(8)2018 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-30071608

RESUMO

Global navigation systems provide worldwide positioning, navigation and navigation services. However, in some challenging environments, especially when the satellite is blocked, the performance of GNSS is seriously degraded or even unavailable. Ground based positioning systems, including pseudolites and Locata, have shown their potentials in centimeter-level positioning accuracy using carrier phase measurements. Ambiguity resolution (AR) is a key issue for such high precision positioning. Current methods for the ground based systems need code measurements for initialization and/or approximating linearization. If the code measurements show relatively large errors, current methods might suffer from convergence difficulties in ground based positioning. In this paper, the concept of double-differential square observation (DDS) is proposed, and an on-the-fly ambiguity resolution (OTF-AR) method is developed for ground based navigation systems using two-way measurements. An important advantage of the proposed method is that only the carrier phase measurements are used, and code measurements are not necessary. The clock error is canceled out by two-way measurements between the rover and the base stations. The squared observations are then differenced between different rover positions and different base stations, and a linear model is then obtained. The floating integer values are easy to compute via this model, and there is no need to do approximate linearization. In this procedure, the rover's approximate coordinates are also directly obtained from the carrier measurements, therefore code measurements are not necessary. As an OTF-AR method, the proposed method relies on geometric changes caused by the rover's motion. As shown by the simulations, the geometric diversity of observations is the key factor for the AR success rate. Moreover, the fine floating solutions given by our method also have a fairly good accuracy, which is valuable when fixed solutions are not reliable. A real experiment is conducted to validate the proposed method. The results show that the fixed solution could achieve centimeter-level accuracy.

15.
Medicine (Baltimore) ; 97(9): e0048, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29489659

RESUMO

RATIONALE: Currently, the use of double-lumen irrigation-suction tube for drainage has become increasingly more common. However, the insertion process is complex, and the position of the double cannula placed in this manner is not accurate. We developed a method for placing the drainage tube and use it in the treatment of an abdominal infection. PATIENT CONCERNS: A 51-year-old man with an abdominal infection due to colonic anastomotic fistula was admitted. Routine laboratory tests revealed an elevated white blood cell count (17 × 10/L) and C-reactive protein level (78 mg/L). Computed tomography (CT) revealed that the peritoneal cavity was filled with fluid. DIAGNOSES: The patient was diagnosed with colonic anastomosis fistula by gastrointestinal radiography. Abdominal infection was diagnosed based on CT scan, inflammatory markers, and patient signs and symptoms. INTERVENTIONS: Two punctures were performed. After skin expansion, the source of infection was drained with a suction catheter (diameter = 1.0 cm) under continuous negative pressure of 150 to 200 millibars, along with continuous saline irrigation at 300 mL/h. OUTCOMES: Pus in the abdomen drained completely. The abdominal infection was controlled. There were no adverse events. LESSONS: Abdominal infection in fistulas is a fatal disease. The main therapeutic target is full drainage at an early stage. Precise positioning of the tube, continuous negative pressure irrigation and drainage are key points in the treatment.


Assuntos
Abscesso Abdominal/terapia , Sucção/métodos , Abscesso Abdominal/etiologia , Doenças do Colo/complicações , Humanos , Fístula Intestinal/complicações , Masculino , Pessoa de Meia-Idade , Sucção/instrumentação , Irrigação Terapêutica/instrumentação , Resultado do Tratamento
16.
Cell Physiol Biochem ; 42(6): 2255-2266, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28817808

RESUMO

BACKGROUND: Impaired wound healing is a common complication of diabetes and is the leading cause of lower extremity amputation. Treatment with fenofibrate, a peroxisome proliferators-activated receptor α (PPARα) agonist, was associated with a lower risk of amputations, particularly minor amputations without known large-vessel diseases, probably through non-lipid mechanisms. The current study aimed to test our hypothesis that fenofibrate stimulates angiogenesis and restores endothelial precursor cell (EPC) function via inhibiting Nod-like receptor protein 3 (NLRP3) inflammasome in streptozotocin (STZ)-induced diabetic mice. METHODS: Male C57BL/6 mice were randomly divided into three groups: control, STZ-induced diabetic mice and fenofibrate treated diabetic group. Wound closure was assessed by wound area and CD31 positive capillaries. Both the migration and tube formation capacities of EPCs were measured. Intracellular nitric oxide (NO) and superoxide (O2-) levels were determined. Activity of NLRP3 inflammasome in EPCs was assessed by measuring thioredoxin-interacting protein (TXNIP), NLRP3, and caspase-1 expression. RESULTS: Compared with the untreated diabetic mice, wound closure and capillary densities were significantly increased in fenofibrate treated group. Fenofibrate treatment restored EPC function, increased NO production, and decreased O2- level in EPCs of diabetic mice. Furthermore, fenofibrate deregulated the activity of NLRP3 inflammasome by reducing TXNIP, NLRP3 and caspase-1 expression in EPCs of diabetic mice. In vitro, fenofibrate prevented high glucose induced EPC dysfunction, deregulated NLRP3 inflammasome activity. In addition, fenofibrate inhibited IL-1ß expression caused by combination use of high glucose and lipopolysaccharide. CONCLUSION: Fenofibrate can accelerate wound healing in diabetic mice, which at least in part was mediated by improving the impaired EPC function via a NLRP3 inflammasome pathway, suggesting the significance of PPARα agonists in the treatment of diabetes.


Assuntos
Fenofibrato/farmacologia , Inflamassomos/metabolismo , Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo , Neovascularização Fisiológica/efeitos dos fármacos , PPAR alfa/agonistas , Transdução de Sinais/efeitos dos fármacos , Animais , Proteínas de Transporte/metabolismo , Caspase 1/metabolismo , Movimento Celular/efeitos dos fármacos , Diabetes Mellitus Experimental/induzido quimicamente , Diabetes Mellitus Experimental/metabolismo , Diabetes Mellitus Experimental/patologia , Células Progenitoras Endoteliais/citologia , Células Progenitoras Endoteliais/efeitos dos fármacos , Células Progenitoras Endoteliais/metabolismo , Ensaio de Imunoadsorção Enzimática , Glucose/toxicidade , Interleucina-1beta/análise , Interleucina-1beta/metabolismo , Lipopolissacarídeos/toxicidade , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Óxido Nítrico/análise , PPAR alfa/metabolismo , Superóxidos/análise , Tiorredoxinas/metabolismo
17.
Chin Med J (Engl) ; 130(2): 199-202, 2017 01 20.
Artigo em Inglês | MEDLINE | ID: mdl-28091412

RESUMO

BACKGROUND: Although that glomerulonephritis is the major cause of end-stage renal disease in developing countries such as China, the increasing prevalence of diabetes has contributed to the changing spectrum of predialysis chronic kidney disease. Recent studies have revealed an increased proportion of patients with diabetic kidney disease (DKD) in hemodialysis populations in large cities in China. However, studies regarding the clinical phenotype of DKD in China are extremely limited. The incidence, development, and prognosis of diabetic kidney disease (INDEED) study aims to investigate the incidence, progression, and prognosis of DKD, as well as the associated genetic, behavioral, and environmental factors and biomarkers in patients with DKD in China. METHODS: INDEED study is a prospective cohort study based on all participants with diabetes in the Kailuan study, which is a general population-based cohort study in northern China. Altogether, over 10,000 participants with diabetes will be followed biennially. Questionnaires documenting general characteristics, behavioral and environmental factors, and medical history will be administrated. Anthropometric measurements and a series of laboratory tests will be performed in one central laboratory. The DNA, plasma, and urine samples of every participant will be stored in a biobank for future research. CONCLUSIONS: INDEED study will provide essential information regarding the clinical phenotype and prognosis of patients with DKD in China and will be valuable to identify factors and biomarkers associated with patients with DKD in China.


Assuntos
Nefropatias Diabéticas/epidemiologia , Biomarcadores , China/epidemiologia , Nefropatias Diabéticas/patologia , Feminino , Humanos , Incidência , Masculino , Prognóstico , Estudos Prospectivos , Inquéritos e Questionários
18.
Medicine (Baltimore) ; 96(3): e5518, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28099329

RESUMO

BACKGROUND: The purpose of this study is to evaluate the efficacy of transcatheter arterial chemoembolization (TACE) combined with computed tomography-guided radiofrequency ablation (CT-RFA) in the treatment of hepatocellular carcinoma (HCC) using magnetic resonance diffusion weighted imaging (MR-DWI) and CT perfusion imaging (CT-PI). METHODS: From January 2008 to January 2014, a total of 522 HCC patients receiving TACE combined with CT-RFA were included in this study. All patients underwent TACE followed by CT-RFA, and 1 day before treatment and 1 month after treatment they received MR-DWI and CT-PI. Enzyme-linked immunosorbent assay (ELISA) was performed to detect the concentration of alpha-fetoprotein (AFP). Tumor response was evaluated using the revised RECIST criteria. One-year follow-up was conducted on all patients. Receiver-operating characteristic (ROC) curve was drawn to evaluate the efficacy of TACE combined with CT-RFA for HCC using MR-DWI and CT-PI. RESULTS: Total effective rate (complete remission [CR] + partial remission [PR]) of TACE combined with CT-RFA for HCC was 82.95%. HCC patients of CR + PR had lower hepatic blood flow (HBF), hepatic blood volume (HBV), permeability surface (PS), hepatic arterial perfusion (HAP), and hepatic perfusion index (HPI) levels than those of SD + PD, but HCC patients of CR + PR had higher mean transit time (MTT) level than those of SD + PD. The patients of PR + CR had higher apparent diffusion coefficient (ADC) values than those of SD + PD. The patients of PR + CR showed lower AFP concentration than those of SD + PD. ROC curve analysis indicated that the area under the curve (AUC) of AFP, HBV, PS, HAP, HPI, and ADC was more than 0.7, but the AUC of HBF, MTT, and PVP were less than 0.7. After treatment, the AFP, HBF, HBV, PS, HAP, and HPI in the HCC patients with recurrence were higher than those in the HCC patients without, but MTT and ADC in the HCC patients with recurrence were lower than those in the HCC patients without. CONCLUSION: These findings indicate that MR-DWI and CT-PI can effectively evaluate the efficacy of TACE combined with CT-RFA and postoperative recurrence of HCC.


Assuntos
Carcinoma Hepatocelular/cirurgia , Ablação por Cateter , Quimioembolização Terapêutica , Neoplasias Hepáticas/cirurgia , Adulto , Idoso , Carcinoma Hepatocelular/diagnóstico por imagem , Terapia Combinada , Imagem de Difusão por Ressonância Magnética , Feminino , Humanos , Neoplasias Hepáticas/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Imagem de Perfusão , Estudos Prospectivos , Tomografia Computadorizada por Raios X
19.
Proc Int AAAI Conf Weblogs Soc Media ; 2017: 704-707, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-31423352

RESUMO

Online health support groups are places for people to compare themselves with others and obtain informational and emotional support about their disease. To do so, they generally need to reveal private information about themselves and in many support sites, they can do this in public or private channels. However, we know little about how the publicness of the channels in health support groups influence the amount of self-disclosure people provide. Our work examines the extent members self-disclose in the private and public channels of an online cancer support group. We first built machine learning models to automatically identify the amount of positive and negative self-disclosure in messages exchanged in this community, with adequate validity (r>0.70). In contrast to findings from non-health-related sites, our results show that people generally self-disclose more in the public channel than the private one and are especially likely to reveal their negative thoughts and feelings publicly. We discuss theoretical and practical implications of our work.

20.
Carbohydr Polym ; 157: 945-952, 2017 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-27988013

RESUMO

Cellulose nanocrystals (CNCs) were prepared from waste cotton cloth and degreasing cotton was used as a comparison. The cellulose was first extracted by alkali and bleaching treatments, and then the CNCs were isolated by the mix acid solution hydrolysis of cellulose under the controlled conditions. The CNCs were analyzed by Attenuated total reflectance Fourier transform infrared spectroscopy, Transmission electron microscopy, X-ray diffraction analysis, thermogravimetric and differential scanning calorimetry analysis. The results confirmed that the resultant samples were the cellulose species, and the CNCs obtained from waste cotton cloth exhibited a high crystallinity index of 55.76±7.82%, which had higher thermostability than that from the degreasing cotton. The morphology analysis results showed that the ranges of length and diameter of CNC extracted from waste cotton cloth were from 28 to 470nm and 3 to 35nm. The preparation of CNCs with a high aspect ratio and good thermostability in this work paves the way for an alternative reuse of waste cotton cloth.

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