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1.
Sci Rep ; 10(1): 3707, 2020 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-32111939

RESUMO

To compare the outcomes of open preperitoneal repair (OPR) with the use of mesh and open suture repair (OSR) without mesh via inguinal approach for the treatment of emergency femoral hernia (FH). The primary outcome was the postoperative complication and the secondary outcomes were the recurrence rate of FH and the postoperative comfort level at the surgical site. 104 patients with emergency FHs were included, of whom 51 patients were treated with OPR, 53 patients were treated with OSR. Between the two groups, no significant difference was found in surgical site infection (SSI) (P = 0.801) or seroma (P = 0.843), while there was significant difference in the improvement of comfort at the surgical site (P = 0.013). The results of the 2-year follow-up demonstrated 1 and 8 cases of recurrence in the OPR and OSR group respectively, which was statistically significant (HR, 8.193 [95% CI, 1.024 to 65.547], P = 0.047). Compared with OSR, OPR with the use of mesh did not increase the risk of SSI and was safe to apply even under the condition of an emergency FH operation with intestine resection; OPR could reduce the recurrence rate of FH and improve the comfort at the surgical site.

2.
Vaccine ; 38(13): 2841-2848, 2020 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-32093983

RESUMO

Transmission-blocking vaccine (TBV) is a promising strategy to interfere with the transmission of malaria. To date, only limited TBV candidate antigens have been identified for Plasmodium vivax. HAP2 is a gamete membrane fusion protein, with homology to the class II viral fusion proteins. Herein we reported the characterization of the PvHAP2 for its potential as a TBV candidate for P. vivax. The HAP2/GCS1 domain of PvHAP2 was expressed in the baculovirus expression system and the recombinant protein was used to raise antibodies in rabbits. Indirect immunofluorescence assays showed that anti-PvHAP2 antibodies reacted only with the male gametocytes on blood smears. Direct membrane feeding assays were conducted using four field P. vivax isolates in Anopheles dirus. At a mean infection intensity of 72.4, 70.7, 51.3, and 15.6 oocysts/midgut with the control antibodies, anti-PvHAP2 antibodies significantly reduced the midgut oocyst intensity by 40.3, 44.4, 61.9, and 89.7%. Whereas the anti-PvHAP2 antibodies were not effective in reducing the infection prevalence at higher parasite exposure (51.3-72.4 oocysts/midgut in the control group), the anti-PvHAP2 antibodies reduced infection prevalence by 50% at a low challenge (15.6 oocysts/midgut). Multiple sequence alignment showed 100% identity among these Thai P. vivax isolates, suggesting that polymorphism may not be an impediment for the utilization of PvHAP2 as a TBV antigen. In conclusion, our results suggest that PvHAP2 could serve as a TBV candidate for P. vivax, and further optimization and evaluation are warranted.

3.
J Cardiothorac Surg ; 15(1): 46, 2020 Feb 27.
Artigo em Inglês | MEDLINE | ID: mdl-32106870

RESUMO

PURPOSE: This study aims to explore whether lobe specific lymph node dissection (LND) is adequate for cN0-1 non-small cell lung cancer (NSCLC) or not. METHODS: Among 5613 cN0-1 NSCLC patients, 394 cases (7.0%) with pN2 were enrolled and the distribution of mediastinal lymph node metastasis was analyzed. The included patients were divided into the non-lobe specific lymph node metastasis (NLSLNM) group and the lobe specific lymph node metastasis (LSLNM) group. The clinicopathological characteristics were compared between two groups and multivariable analysis was performed to find independent factors predicting NLSLNM. RESULTS: The incidence of pN2 cases deserved serious attention. The proportion of upper zone lymph node metastases was not rare in right (55.0%) and left (35.7%) lower lobe tumors. The proportion of subcarinal zone lymph node involvement was also high in right (21.8%) and left (25.8%) upper lobe tumors. Multivariable analysis showed that elevated carcinoembryonic antigen (CEA) level (P = 0.034), right lower lobe (RLL) tumors (P = 0.022) and station 11 involvement (P = 0.030) were independent risk factors for NLSLNM. CONCLUSION: Systematic LND seems to be superior to lobe specific LND in the assessment of lymph node status and high CEA level, RLL tumors and station 11 involvement are predictors for NLSLNM.

4.
Medicine (Baltimore) ; 99(8): e19317, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32080149

RESUMO

Endovenous laser ablation (EVLA) and ultrasound-guided foam sclerotherapy (UGFS) have largely replaced traditional surgery for treatment of varicose veins (VVs) with active venous leg ulcers (VLUs), and multiple combined modes have emerged. A retrospective cohort study was performed to compare the effect of traditional surgery (high ligation and stripping followed with compression [compression plus HL-S]) to high ligation-endovenous laser ablation-foam sclerotherapy followed with compression (compression plus HL-EVLA-FS) on the treatment of active VLUs.Data of active VLUs treated in our center from 2008 to 2017 and followed up for 1 year were analyzed. The intervention measures in the first 5 years were compression plus HL-S, and in the following 5 years were compression plus HL-EVLA-FS. The primary outcome was ulcer healing time. The secondary outcomes were the VVs occlusion and clinical success as assessed by a change in venous clinical severity score (VCSS) and complications.The study included 120 patients and 200 patients treated with HL-S and HL-EVLA-FS, respectively, during 2008 to 2017. The average ulcer healing time were 2.3 ±â€Š2.4 and 1.7 ±â€Š1.7 months, respectively. Significant difference was found in the cumulative ulcers healing rate between the two groups (Hazard ratio [HR] and 95% confidence interval [CI] was respectively 1.458 and 1.140-1.865, P = .0002), but no difference was found in the VVs occlusion (HR and 95% CI was respectively 1.005 and 0.774-1.3071, P = .967). Significant difference occurred in 6 months and 12 months post-operatively in the VCSS change and in the procedure data and some complications between the 2 groups.In conclusion, the treatment of HL-EVLA-FS can accelerate the healing of VLUs, improve the VCSS and present superior procedure data. However, no advantage could be found in the VVs occlusion compared with control group.


Assuntos
Terapia a Laser , Ligadura , Escleroterapia , Úlcera Varicosa/terapia , Procedimentos Cirúrgicos Vasculares , Estudos de Coortes , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Veia Safena/cirurgia , Índice de Gravidade de Doença , Meias de Compressão , Cicatrização
5.
World J Microbiol Biotechnol ; 36(2): 33, 2020 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-32060755

RESUMO

Microbiota from herbivore rumen is of great interest for mining glycoside hydrolases for lignocellulosic biomass biorefinement. We previously isolated a highly active but poorly thermostable xylanase (LXY) from a rumen fluid fosmid library of Hu sheep, a local high-reproductive species in China. In this study, we used a universal enzyme-engineering strategy called SpyTag/SpyCatcher molecular cyclization to improve LXY stability via isopeptide-bond-mediated ligation. Both linear and cyclized LXY (L- and C-LXY, respectively) shared similar patterns of optimal pH and temperature, pH stability, and kinetic constants (km and Vmax). However, the C-LXY showed enhanced thermostability, ion stability, and resilience to aggregation and freeze-thaw treatment than L-LXY, without compromise of its catalytic efficiency. Circular dichroism and intrinsic and 8-anilino-1-naphthalenesulfonic acid-binding fluorescence analysis indicated that the cyclized enzyme was more capable of maintaining its secondary and tertiary structures than the linear enzyme. Taken together, these results promote the cyclized enzyme for potential applications in the feed, food, paper pulp, and bioenergy industries.

6.
Surg Endosc ; 2020 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-32072277

RESUMO

BACKGROUND: We analyzed the pathological characteristics and recurrence pattern of cN0 submucosal esophageal cancer after esophagectomy and conducted risk stratification to determine the feasibility of performing endoscopic resection for cN0pT1b esophageal squamous cell malignancies. METHODS: We retrospectively enrolled 167 patients who underwent right-sided transthoracic esophagectomy and extended thoracic/abdominal two-field lymphadenectomy. Patients with pathologically confirmed lymph node metastasis or tumor recurrence constituted the high-risk group for endoscopic submucosal resection, and the remainder were defined as low risk. Factors affecting lymphatic metastasis and long-term recurrence were identified by univariate and multivariate analyses. RESULTS: Postoperative pathology showed that five patients (5/167; 3%) had lymph node metastases. Follow-up ranged from 12-60 months, with a median of 29 months. A total of 17 patients (10.2%) had recurrences during follow-up, including three patients with pathologic nodal metastasis (pN +) found at surgery. Invasion depth, differentiation, and tumor size differed significantly in high-risk patients. Overall 3-year survival rates were 94.2% (low-risk) and 40.9% (high-risk) (p < 0.01). Twenty-one patients with sm1 cancer, high tumor differentiation, and tumor length < 2 cm had no lymph node metastasis or lymphovascular invasion, and none of these patients experienced recurrence. CONCLUSIONS: Endoscopic submucosal resection alone may be feasible for patients with small (≤ 2 cm) clinically N0 submucosal esophageal squamous cell carcinoma with low invasion depth (sm1) and higher differentiation, but prospective studies are required for confirmation. Other patients require surgical resection with extended two-field thoracic/abdominal lymphadenectomy.

7.
BMC Cardiovasc Disord ; 20(1): 32, 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-31992206

RESUMO

BACKGROUND: The gut microbiome plays an important role in various cardiovascular diseases, such as atherosclerosis and hypertension, which are associated with abdominal aortic aneurysms (AAAs). METHODS: Here, we used 16S rRNA sequencing to explore gut microbiota in C57BL ApoE-/- mice with AAAs. A mouse model of abdominal aortic aneurysms was induced with angiotensin II (Ang II) (1000 ng/min per kg). On day 28 after the operation, fecal samples were collected and stored at - 80 °C until DNA extraction. We determined the relative abundances of bacterial taxonomic groups using 16S rRNA amplicon metabarcoding, and sequences were analyzed using a combination of mother software and UPARSE. RESULTS: We found that the gut microbiome was different between control and AAA mice. The results of correlation analysis between AAA diameter and the gut microbiome as well as LEfSe of the genera Akkermansia, Odoribacter, Helicobacter and Ruminococcus might be important in the progression of AAAs. CONCLUSIONS: AAA mice is subjected to gut microbial dysbiosis, and gut microbiota might be a potential target for further investigation.

8.
Nanotechnology ; 31(12): 125402, 2020 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-31770723

RESUMO

In this work, a cerium doped CoP nanoparticles (NPs) embedded in carbon nanotubes (CNTs) for efficient and durable hydrogen evolution was developed. The detailed preparation process was described as the followings. First, cerium was introduced into ZIF-67 to form Ce-doped ZIF-67 by a joint nucleation method. Then, the Ce-doped Co-CNTs was synthesized by carbonization of Ce-doped ZIF-67. During the process, the Co2+ was reduced to form Co NPs and the elegant nanostructure of CNTs was formed by the catalytic effect of Co NPs. Finally, by using Ce-doped Co-CNTs as the precursor, the target catalyst (Ce0.05-doped CoP CNTs) was obtained through a chemical vapour deposition (CVD) process in the presence of NaH2PO2. Results of the transmission electron microscopy (TEM) and scanning electron microscopy (SEM) showed that the target catalyst maintained the original rhombic dodecahedron morphology of ZIF-67 and the CoP NPs were embedded in CNTs and distributed uniformly throughout the catalyst. In electrochemical measurements, the catalyst showed the best performance for HER in 0.5 M H2SO4 solution. The onset potential, Tafel slope, electron transfer resistance (R ct) and double-layer capacitance (C dl) of the target catalyst was 49 mV, 78 mV dec-1, 19.2 Ω and 10.5 mF cm-2, respectively. Meanwhile, the catalyst yielded a current density of 10 mA cm-2 merely at an overpotential of 146 mV. Furthermore, it maintained 90% of the original current density in a chronoamperometry measurement and showed no obvious decay even after 2000 cycles scans in a long-term durability test.

9.
J Microbiol Methods ; 168: 105780, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31751598

RESUMO

The CapitalBio™ Mycobacterium nucleic acid detection test is a real-time fluorescent polymerase chain reaction and TaqMan probe technology test for rapid diagnosis of extrapulmonary tuberculosis (EPTB). This test had moderate sensitivity and high specificity for EPTB, like that of Xpert MTB/RIF, especially for lymph node, chest wall, and purulent fluid samples.

10.
Am J Physiol Heart Circ Physiol ; 318(1): H124-H134, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31834836

RESUMO

Elabela (ELA) is a newly discovered peptide that acts as a novel endogenous ligand of angiotensin receptor-like 1 (APJ) receptor. This study was designed to evaluate the effects of ELA-21 in paraventricular nucleus (PVN) on blood pressure and sympathetic nerve activity in spontaneously hypertensive rats (SHR). Experiments were performed in male Wistar-Kyoto rats (WKY) and SHR. ELA expression was upregulated in PVN of SHR. PVN microinjection of ELA-21 increased renal sympathetic nerve activity (RSNA), mean arterial pressure (MAP), heart rate (HR), plasma norepinephrine, and arginine vasopressin (AVP) levels in SHR. Intravenous injection of ELA-21 significantly decreased MAP and HR in both WKY and SHR, but only induced a slight decrease in RSNA. APJ antagonist F13A in PVN abolished the effects of ELA-21 on RSNA, MAP and HR. Intravenous infusion of both ganglionic blocker hexamethonium and AVP V1a receptor antagonist SR49059 caused significant reduction in the effects of ELA-21 on RSNA, MAP and HR in SHR, while combined administration of hexamethonium and SR49059 abolished the effects of ELA-21. ELA-21 microinjection stimulated Akt and p85α subunit of phosphatidylinositol 3-kinase (PI3K) phosphorylation in PVN, whereas PI3K inhibitor LY294002 or Akt inhibitor MK-2206 almost abolished the effects of ELA-21 on RSNA, MAP, and HR. Chronic PVN infusion of ELA-21 induced sympathetic activation, hypertension, and AVP release accompanied with cardiovascular remodeling in normotensive WKY. In conclusion, ELA-21 in PVN induces exacerbated pressor and sympathoexcitatory effects in hypertensive rats via PI3K-Akt pathway.NEW & NOTEWORTHY We demonstrated that PVN microinjection of ELA-21 increases sympathetic nerve activity and blood pressure, which can be abolished by pretreatment of APJ antagonist. This is the first demonstration that central ELA can induce hypertension. The pressor effects in PVN are mediated by both sympathetic activation and vasopressin release via PI3K-Akt pathway. Our data confirm that ELA is upregulated in the PVN of SHR and so may be involved in the pressor and sympathoexcitatory effects in hypertension.

11.
Medicine (Baltimore) ; 98(49): e18192, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31804338

RESUMO

RATIONALE: Tension-free repair of inguinal hernia with prosthetic materials in adults has become a routine surgical procedure. However, serious complications may arise such as mesh displacement, infection, and even enterocutaneous fistula (EF). The management of chronic mesh infection (CMI) complicated by an EF is very challenging. A simple treatment of infected mesh removal and negative pressure wound therapy (NPWT) may cure the patients with EF with CMI. PATIENT CONCERNS: A 75-year-old male patient underwent tension-free treatment for a bilateral inguinal hernia at a county hospital 10 years ago. Three months before admission, the right groin gradually formed a skin sinus with outflow of fetid thin pus, and it could not heal. DIAGNOSES: The patient was diagnosed preoperatively with mesh plug adhesion to the intestine, which resulted in low-flow EF combined with CMI. INTERVENTIONS: The patient received a simple treatment mode consisting of an incision made from the original incision, but the new incision did not penetrate the abdominal cavity; treatment included resection of the fistula, removal of the mesh, repair of the intestine and local tissue, and continuous irrigation of vacuum sealing drainage (VSD) devices for NPWT. OUTCOMES: The infected mesh was completely removed. Five VSD devices were utilized to treat the EF and wound. The time from intervention to wound healing was 35 days, and follow-up for 6 months revealed no infection and no hernia recurrence in the right groin. LESSONS: The NPWT is effective in treating CMI concomitant with EF and does not increase the risk of hernia recurrence.


Assuntos
Hérnia Inguinal/cirurgia , Herniorrafia/efeitos adversos , Fístula Intestinal/cirurgia , Infecção da Ferida Cirúrgica/cirurgia , Idoso , Remoção de Dispositivo , Herniorrafia/métodos , Humanos , Masculino , Tratamento de Ferimentos com Pressão Negativa/métodos , Telas Cirúrgicas/microbiologia
12.
Med Clin (Barc) ; 2019 Dec 09.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-31831150
13.
Int Health ; 11(S1): S24-S32, 2019 10 31.
Artigo em Inglês | MEDLINE | ID: mdl-31670821

RESUMO

BACKGROUND: According to the 2017 China National Education Development Statistics Bulletin, there were 14.07 million rural-urban migrant children in the compulsory education stage. The mental health of migrant children in China has drawn increasing attention in research. The objective of this study was to compare subjective well-being, self-esteem, prosocial behaviour and family functioning of migrant children vs local children in Shanghai and to explore their relationship. METHODS: A survey was conducted among 2229 students (9-17 y of age; male 52.0%, female 48.0%) and their parents from grades 4 to 8 in four primary schools and four middle schools in Shanghai in 2016. The sample consisted of 1333 migrant children and 896 urban children in three migrant schools and five public schools. A total of 959 rural-urban migrant children and 374 urban hukou migrant children were recruited. The questionnaire for students included the Personal Well-Being Index - School Children (PWI-SC), Satisfaction with Life Scale (SWLS), Positive and Negative Affect Scale for Children, Rosenberg Self-Esteem Scale, Family APGAR Index and prosocial behaviour domain of the Strengths and Difficulties Questionnaire. In the questionnaire for parents, the Personal Well-Being Index and Social Support Rating Scale were used in addition to the sociodemographic characteristics. These data were analysed using one-way analysis of variance, correlation analysis and multiple linear regression. RESULTS: Rural-urban migrant children reported significantly lower PWI-SC, SWLS, positive affect, prosocial behaviour, self-esteem and Family APGAR Index scores and reported higher negative affect scores than local children (p<0.01). The prevalence rate of abnormal prosocial behaviour among rural-urban migrant children was 10%, which was higher than that of local children (5.9%; p<0.001). Compared with rural-urban migrant children in public schools, the SWLS, prosocial behaviour and Family APGAR Index scores of the children in migrant schools were higher and the prevalence rate of abnormal prosocial behaviour was lower (p<0.01). Multiple linear regression analysis indicated that the self-esteem and Family APGAR Index had predictive effects on subjective well-being and prosocial behaviour scores of rural-urban migrant children. CONCLUSIONS: Rural-urban migrant children are susceptible to mental health problems. Additional public policy and interventions by practitioners are needed to support rural-urban migrant children.


Assuntos
Relações Familiares/psicologia , Saúde Mental/estatística & dados numéricos , População Rural/estatística & dados numéricos , Estudantes/psicologia , Estudantes/estatística & dados numéricos , Migrantes/psicologia , População Urbana/estatística & dados numéricos , Adolescente , Criança , China/epidemiologia , Cidades/estatística & dados numéricos , Feminino , Humanos , Masculino , Prevalência , Migrantes/estatística & dados numéricos
14.
Int Health ; 11(S1): S45-S54, 2019 10 31.
Artigo em Inglês | MEDLINE | ID: mdl-31670822

RESUMO

BACKGROUND: A cross-sectional study was conducted in 2018 to investigate the mental health status of internal migrant workers (IMWs) in Shanghai to understand basic information and the mental health status of IMWs. METHODS: The total number of IMWs in the study was 4793 and 4648 questionnaires were valid. We used χ2 test, t test, analysis of variance, linear regression and logistic regression to analyse the data. RESULTS: Mental health, represented by the nine-item Patient Health Questionnaire score (≥10), was significantly correlated with self-rated income-expenditure status, living with family, job type and alcohol use. The total depression prevalence of IMWs in this study was 20.1%. Subjective well-being (SWB) was significantly correlated with marital status, educational attainment, self-reported income-expenditure status, living with family and job type. Those who were female, had a high school education or above, self-reported inadequate income, did not live with family, had poor self-rated health and had moderate or severe depression were more likely to have lower SWB. Promoting the mental health of IMWs also benefited their physical health. CONCLUSIONS: Chinese migrant workers who were younger, had insufficient self-rated income, had worse self-reported health, used alcohol and were unmarried had a high risk of mental health disorders.


Assuntos
Transtorno Depressivo/epidemiologia , Nível de Saúde , Saúde Mental/estatística & dados numéricos , Adulto , China/epidemiologia , Estudos Transversais , Análise Fatorial , Feminino , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Prevalência , Autorrelato , Inquéritos e Questionários , Migrantes
15.
Mediators Inflamm ; 2019: 8461725, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31686986

RESUMO

Sepsis-associated encephalopathy (SAE) is a poorly understood condition that leads to long-term cognitive impairment and increased mortality in survivors. Recent research revealed that IL-17A/IL-17R might serve as a checkpoint in microglia-mediated neuroinflammation. The present study was designed to determine the specific role of IL-17A-mediated microglia activation in the development of SAE. A mouse model of SAE was induced by cecal ligation and puncture (CLP), and behavior performance was evaluated by the inhibitory avoidance test and the open field test. Cytokine expression and microglia activation in brain tissue were determined at 6 h, 12 h, 24 h, 48 h, and day 7 post surgery. Further, septic mice were intracerebral ventricle- (i.c.v.-) injected with recombinant IL-17A, anti-IL-17A ab, anti-IL-17R ab, or isotype controls to evaluate the potential effects of IL-17A/IL-17R blockade in the prevention of SAE. Septic peritonitis induced significant impairment of learning memory and exploratory activity, which was associated with a higher expression of IL-17A, IL-1ß, and TNF-α in the brain homogenate. Fluorescence intensity of Iba-1 and IL-17R in the hippocampus was significantly increased following CLP. Treatment with recombinant IL-17A enhanced the neuroinflammation and microglia activation in CLP mice. On the contrary, neutralizing anti-IL-17A or anti-IL-17R antibodies mitigated the CNS inflammation and microglia activation, thus alleviating the cognitive dysfunction. Furthermore, as compared to the sham control, microglia cultured from CLP mice produced significantly higher levels of cytokines and expressed with higher fluorescence intensity of Iba-1 in response to IL-17A or LPS. Pretreatment with anti-IL-17R ab suppressed the Iba-1 expression and cytokine production in microglia stimulated by IL-17A. In conclusion, blockade of the IL-17A/IL-17R pathway inhibited microglia activation and neuroinflammation, thereby partially reversing sepsis-induced cognitive impairment. The present study suggested that the IL-17A/IL-17R signaling pathway had an important, nonredundant role in the development of SAE.

16.
Math Biosci Eng ; 16(6): 7659-7670, 2019 08 21.
Artigo em Inglês | MEDLINE | ID: mdl-31698632

RESUMO

Objectives: The purpose of this meta-analysis was to evaluate the efficacy and toxicity profile of apatinib for the treatment of advanced non-small cell lung cancer (NSCLC). Methods: We systematically searched databases for randomized clinical trials published as of November 25, 2017, in which apatinib treatment was compared to placebo or chemotherapy in patients with advanced NSCLC. Two investigators independently assessed the articles and extracted their data. The hazard ratios (HRs) for progression-free survival (PFS), relative risks (RRs) for overall response rates (ORRs), disease control rates (DCRs), and odds ratios (ORs) for main toxicity were analyzed using the RevMan 5.3 software (Copenhagen: The Nordic Cochrane Centre, The Cochrane Collaboration, 2014). Results: Our analysis included 413 patients from 5 clinical studies. The pooled HR for PFS was 0.32 (95% confidence interval (CI) 0.21-0.48; P < 0.00001). The pooled RRs for ORR and DCR were 2.03 (95% CI 1.36-3.01; P = 0.0005) and 1.66 (95% CI 1.07-2.57; P = 0.02), respectively. The pooled OR for main toxicity was 1.34 (95% CI, 0.57-3.17; P = 0.5). Conclusions: Apatinib was a viable treatment alternative for advanced NSCLC, as it offered a clinically meaningful and statistically significant improvement in PFS, ORR, and DCR. Moreover, therapy with apatinib did not significantly increase toxicity.

17.
Sci Rep ; 9(1): 14021, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31575998

RESUMO

We aimed to assess the ulcer healing time and recurrence rates after treatment with compression therapy (CT) with or without high ligation-endovenous laser ablation-foam sclerotherapy (HL-EVLA-FS) in people with active venous leg ulcers (VLUs). A retrospective cohort study was conducted with 350 patients with active VLUs treated by compression with or without HL-EVLA-FS in our hospital from 2013 to 2017. The primary outcome was the ulcer healing time; secondary outcomes were the 12-month recurrence rates, the relationship between recurrence and venous reflux, and the complications of the two treatments. In total, 193 patients (200 limbs) underwent compression plus HL-EVLA-FS, and 157 patients (177 limbs) underwent CT alone. The ulcer healing time was shorter in the compression plus HL-EVLA-FS group than in the CT alone group (Hazard Ratio [HR] for ulcer healing, 1.845 [95% CI, 1.474-2.309], P = 0.0001). The 12-month ulcer recurrence rates were significantly reduced in the compression plus HL-EVLA-FS group (HR for ulcer recurrence, 0.418 [95% CI, 0.258-0.677], P = 0.0001). Calf perforator vein reflux (CPVR) and isolated superficial venous reflux (ISVR) were risk factors for ulcer recurrence. The combined operation with CT resulted in faster healing of VLUs, a lower ulcer recurrence rate and lower VCSS values after intervention than CT alone.

18.
RNA Biol ; : 1-8, 2019 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-31607216

RESUMO

Non-coding RNAs occupy a significant fraction of the human genome. Their biological significance is backed up by a plethora of emerging evidence. One of the most robust approaches to demonstrate non-coding RNA's biological relevance is through their prognostic value. Using the rich gene expression data from The Cancer Genome Altas (TCGA), we designed Advanced Expression Survival Analysis (AESA), a web tool which provides several novel survival analysis approaches not offered by previous tools. In addition to the common single-gene approach, AESA computes the gene expression composite score of a set of genes for survival analysis and utilizes permutation test or cross-validation to assess the significance of log-rank statistic and the degree of over-fitting. AESA offers survival feature selection with post-selection inference and utilizes expanded TCGA clinical data including overall, disease-specific, disease-free, and progression-free survival information. Users can analyse either protein-coding or non-coding regions of the transcriptome. We demonstrated the effectiveness of AESA using several empirical examples. Our analyses showed that non-coding RNAs perform as well as messenger RNAs in predicting survival of cancer patients. These results reinforce the potential prognostic value of non-coding RNAs. AESA is developed as a module in the freely accessible analysis suite MutEx. Abbreviation: ACC: Adrenocortical Carcinoma (n = 92); BLCA: Bladder Urothelial Carcinoma (n = 412); BRCA: Breast Invasive Carcinoma (n = 1098); CESC: Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma (n = 307); CHOL: Cholangiocarcinoma (n = 51); COAD: Colon Adenocarcinoma (n = 461); DLBC: Lymphoid Neoplasm Diffuse Large B-cell Lymphoma (n = 58); ESCA: Oesophageal Carcinoma (n = 185); GBM: Glioblastoma Multiforme (n = 617); HNSC: Head and Neck Squamous Cell Carcinoma (n = 528); KICH: Kidney Chromophobe (n = 113); KIRC: Kidney Renal Clear Cell Carcinoma (n = 537); KIRP: Kidney Renal Papillary Cell Carcinoma (n = 291); LAML: Acute Myeloid Leukaemia (n = 200); LGG: Brain Lower Grade Glioma (n = 516); LIHC: Liver Hepatocellular Carcinoma (n = 377); LUAD: Lung Adenocarcinoma (n = 585); LUSC: Lung Squamous Cell Carcinoma (n = 504); MESO: Mesothelioma (n = 87); OV: Ovarian Serous Cystadenocarcinoma (n = 608) PAAD: Pancreatic Adenocarcinoma (n = 185); PCPG: Pheochromocytoma and Paraganglioma (n = 179); PRAD: Prostate Adenocarcinoma (n = 500); READ: Rectum Adenocarcinoma (n = 172); SARC: Sarcoma (n = 261); SKCM: Skin Cutaneous Melanoma (n = 470); STAD: Stomach Adenocarcinoma (n = 443); TGCT: Testicular Germ Cell Tumours (n = 150); THCA: Thyroid Carcinoma (n = 507) THYM: Thymoma (n = 124); UCEC: Uterine Corpus Endometrial Carcinoma (n = 560); UCS: Uterine Carcinosarcoma (n = 57); UVM: Uveal Melanoma (n = 80).

19.
Wei Sheng Yan Jiu ; 48(4): 537-544, 2019 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-31601353

RESUMO

OBJECTIVE: To revise the Measurement Properties of Neighborhood Scales(MPNS), and to evaluate their reliability and validity. METHODS: Seven thousand seven hundred and twenty-nine community-based residents aged 18-75 years were selected by stratified random sampling, and gender, age, educational level, urban-suburban area and self-rated health were documented. The community attributes were assessed with the Chinese version of MPNS, which consists of 24 items, and 4 dimension including aesthetic quality, walking environment, social cohesion, and social participation. The scale were revised using exploratory factor analysis, and the reliability and validity of the revised scale were tested among community-based adults in different areas and different ages. RESULTS: The scale was revised from 24 items, 4 dimension to 27 items, 6 dimension, which included street environment, aesthetic quality, walking environment, social cohesion, social participation and social connection, and could explain 68. 9% of the total variance. The Cronbach's α of revised scale was 0. 89 and the Cronbach's α of each subscales were range from 0. 51 to 0. 92, the split-half reliability was 0. 91. The revised scale had favorable discriminant validity and construct validity. In addition, various parameters of the confirmatory factor analysis were all acceptable. CONCLUSION: Revised Chinese version of MPNS is reliable and valid among community-based adults⇿5 years.


Assuntos
Relações Interpessoais , Características de Residência , Adolescente , Adulto , Idoso , Análise Fatorial , Humanos , Pessoa de Meia-Idade , Reprodutibilidade dos Testes , Inquéritos e Questionários , Adulto Jovem
20.
J Transl Med ; 17(1): 256, 2019 08 07.
Artigo em Inglês | MEDLINE | ID: mdl-31391086

RESUMO

BACKGROUND: The adipose afferent reflex (AAR), a sympatho-excitatory reflex, can promote the elevation of sympathetic nerve activity (SNA) and blood pressure (BP). Inflammation in the paraventricular nucleus (PVN) involves sympathetic abnormality in some cardiovascular diseases such as hypertension. This study was designed to explore the effects of tumor necrosis factor alpha (TNFα) in the PVN on the AAR and SNA in rats with obesity-related hypertension (OH) induced by a high-fat diet for 12 weeks. METHODS: Renal sympathetic nerve activity (RSNA) and mean arterial pressure (MAP) were continuously recorded in anesthetized rats, and their responses to capsaicin (CAP) stimulation of the right inguinal white adipose tissue were used to evaluate the AAR. RESULTS: Compared to the control rats, the systolic blood pressure (SBP), plasma norepinephrine (NE, indicating SNA) and TNFα levels, TNFα mRNA and protein levels, reactive oxygen species (ROS) content and NADPH oxidase activity in the PVN were significantly elevated in rats with OH. TNFα in the PVN markedly enhanced sympathoexcitation and AAR. Moreover, the enhancement of AAR caused by TNFα can be significantly strengthened by the pretreatment of diethyldithiocarbamate (DETC), a superoxide dismutase inhibitor, but attenuated by TNF-α receptor antagonist R-7050, superoxide scavenger PEG-SOD and NADPH oxidase inhibitor apocynin (Apo) in rats with OH. Acute microinjection of TNF-α into the PVN significantly increased the activity of NADPH oxidase and ROS levels in rats with OH, which were effectively blocked by R-7050. Furthermore, our results also showed that the increased levels of ROS, TNFα and NADPH oxidase subunits mRNA and protein in the PVN of rats with OH were significantly reversed by pentoxifylline (PTX, 30 mg/kg daily ip; in 10% ethanol) application, a cytokine blocker, for a period of 5 weeks. PTX administration also significantly decreased SBP, AAR and plasma NE levels in rats with OH. CONCLUSIONS: TNFα in the PVN modulates AAR and contributes to sympathoexcitation in OH possibly through NADPH oxidase-dependent ROS generation. TNFα blockade attenuates AAR and sympathoexcitation that unveils TNFα in the PVN may be a possible therapeutic target for the intervention of OH.

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