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J Colloid Interface Sci ; 560: 1-10, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31630023


Glucose is a popular biosensor target due to its closely with diabetes or hypoglycemia in blood. Designing efficiency electrocatalysts for the determination of glucose is vital to develop glucose detection devices. CoMoO4, as a kind of bimetallic oxide material, exhibits unique electrochemical properties. 3D macroporous carbon (MPC) has large specific surface area and excellent electrical conductivity, providing an effective support for loading other nano-entities to form novel composite with good synergetic effects. Herein, nanorod-like CoMoO4 anchored onto MPC support was synthesized for the development of a promising electrochemical sensing platform for glucose. Attributing to the synergic effects between the good electrocatalytic performance of CoMoO4 nanorods and the extraordinary electrical conductivity of 3D layered MPC, the novel CoMoO4/MPC composites non-enzymatic sensor shows excellent electrocatalytic performance for oxidation of glucose. Under the optimum conditions, the proposed CoMoO4/MPC hybrids provided a reliable linear range of 5 × 10-7 to 1.08 × 10-4 M with a low limit of detection (0.13 µM) for the detection of glucose. Meanwhile, the CoMoO4/MPC sensing platform shows fast response time of 1.76 s, good stability and selectivity for detecting glucose. Moreover, this non-enzymatic sensor also has been successfully applied to measure glucose level in human blood samples. Therefore, the developed sensor holds a new promise for the construction of facile and sensitive non-enzymatic glucose analytical platform.

Appl Microbiol Biotechnol ; 101(18): 6981-6992, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28762001


This study evaluated the effects of high-grain diets on the rumen fermentation, epithelial bacterial community, morphology of rumen epithelium, and local inflammation of goats during high-grain feeding. Twelve 8-month-old goats were randomly assigned to two different diets, a hay diet or a high-grain diet (65% grain, HG). At the end of 7 weeks of treatment, samples of rumen content and rumen epithelium were collected. Rumen pH was lower (P < 0.05), but the levels of volatile fatty acids and lipopolysaccharides were higher (P < 0.05) in the HG group than those in the hay group. The principal coordinate analysis indicated that HG diets altered the rumen epithelial bacterial community, with an increase in the proportion of genus Prevotella and a decrease in the relative abundance of the genera Shuttleworthia and Fibrobacteres. PICRUSt analysis suggested that the HG-fed group had a higher (P < 0.05) relative abundance of gene families related to energy metabolism; folding, sorting, and degradation; translation; metabolic diseases; and immune system. Furthermore, HG feeding resulted in the rumen epithelial injury and upregulated (P < 0.05) the gene expressions of IL-1ß and IL-6, and the upregulations were closely related to the rumen pH, LPS level, and rumen epithelial bacteria abundance. In conclusion, our results indicated that the alterations in the rumen environment and epithelial bacterial community which were induced by HG feeding may result in the damage and local inflammation in the rumen epithelium, warranting further study of rumen microbial-host interactions in the HG feeding model.

Ração Animal/efeitos adversos , Bactérias/isolamento & purificação , Grão Comestível/efeitos adversos , Cabras/microbiologia , Microbiota , Animais , Clostridiales/isolamento & purificação , Citocinas/metabolismo , Dieta/efeitos adversos , Dieta/veterinária , Epitélio/metabolismo , Epitélio/microbiologia , Ácidos Graxos Voláteis/análise , Fermentação , Cabras/metabolismo , Inflamação/veterinária , Mucosa Intestinal/metabolismo , Mucosa Intestinal/microbiologia , Lipopolissacarídeos/análise , Masculino , Prevotella/isolamento & purificação , Distribuição Aleatória , Rúmen/metabolismo , Rúmen/microbiologia
J Sep Sci ; 39(16): 3239-45, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27378629


A simple and highly efficient interface to couple capillary electrophoresis with inductively coupled plasma mass spectrometry by a microflow polyfluoroalkoxy nebulizer and a quadruple ion deflector was developed in this study. By using this interface, six arsenic species, including arsenite, arsenate, monomethylarsonic acid, dimethylarsinic acid, arsenobetaine, and arsenocholine, were baseline-separated and determined in a single run within 11 min under the optimized separation conditions. The instrumental detection limit was in the range of 0.02-0.06 ng/mL for the six arsenic compounds. Repeatability expressed as the relative standard deviation (n = 5) of both migration time and peak area were better than 2.5 and 4.3% for six arsenic compounds. The proposed method, combined with a closed-vessel microwave-assisted extraction procedure, was successfully applied for the determination of arsenic species in the Solanum Lyratum Thunb samples from Anhui province in China with the relative standard deviations (n = 5) ≤4%, method detection limits of 0.2-0.6 ng As/g and a recovery of 98-104%. The experimental results showed that arsenobetaine was the main speciation of arsenic in the Solanum Lyratum Thunb samples from different provinces in China, with a concentration of 0.42-1.30 µg/g.

Arsenicais/química , Espectrometria de Massas/métodos , Solanum/química , China , Eletroforese Capilar
Sci Rep ; 6: 20329, 2016 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-26841945


Remarkably little information is available about the impact of high-grain (HG) feeding on colonic mucosa-associated bacteria and mucosal morphology. In the present study, 12 male goats were randomly assigned to either a hay diet (n = 6) or an HG diet (65% grain; n = 6) to characterise the changes in the composition of the bacterial community in colonic mucosa and the mucosal morphology of the colon. The results showed that HG feeding decreased the colonic pH and increased the concentrations of total short chain fatty acids and lipopolysaccharides in colonic digesta. The principal coordinate analysis results showed that the HG diet altered the colonic mucosal bacterial communities, with an increase in the abundance of genus Blautia and a decrease in the abundance of genera Bacillus, Enterococcus, and Lactococcus. The HG-fed goats showed sloughing of the surface layer epithelium, intercellular tight junction erosion, cell mitochondrial damage, and upregulation of the relative mRNA expression of IL-2 and IFN-γ in colonic mucosa. Collectively, our data indicate that HG feeding induced changes in colonic mucosal morphology and cytokines expression that might be caused by excessive fermentation and dramatic shifts in the bacterial populations in the colon.

Colo/microbiologia , Grão Comestível/efeitos adversos , Mucosa Intestinal/microbiologia , Ração Animal/efeitos adversos , Animais , Bacteroidetes/genética , Bacteroidetes/isolamento & purificação , Clostridiales/genética , Clostridiales/isolamento & purificação , Colo/metabolismo , Colo/patologia , Ácidos Graxos/metabolismo , Cabras , Concentração de Íons de Hidrogênio , Interferon gama/genética , Interferon gama/metabolismo , Interleucina-2/genética , Interleucina-2/metabolismo , Mucosa Intestinal/metabolismo , Mucosa Intestinal/patologia , Lipopolissacarídeos/metabolismo , Masculino , Microbiota , Proteobactérias/genética , Proteobactérias/isolamento & purificação , RNA Ribossômico 16S/química , RNA Ribossômico 16S/genética , RNA Ribossômico 16S/metabolismo , Análise de Sequência de DNA , Spirochaetales/genética , Spirochaetales/isolamento & purificação , Junções Íntimas/patologia
AMIA Annu Symp Proc ; 2011: 1573-82, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-22195223


In order to support the increasing need to share electronic health data for research purposes, various methods have been proposed for privacy preservation including k-anonymity. Many k-anonymity models provide the same level of anoymization regardless of practical need, which may decrease the utility of the dataset for a particular research study. In this study, we explore extensions to the k-anonymity algorithm that aim to satisfy the heterogeneous needs of different researchers while preserving privacy as well as utility of the dataset. The proposed algorithm, Attribute Utility Motivated k-anonymization (AUM), involves analyzing the characteristics of attributes and utilizing them to minimize information loss during the anonymization process. Through comparison with two existing algorithms, Mondrian and Incognito, preliminary results indicate that AUM may preserve more information from original datasets thus providing higher quality results with lower distortion.

Algoritmos , Pesquisa Biomédica , Confidencialidade , Sistemas Computadorizados de Registros Médicos , Segurança Computacional , Health Insurance Portability and Accountability Act , Estados Unidos