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1.
Inorg Chem ; 2020 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-32401023

RESUMO

An alkaline-earth-metal iodate fluoride, Ba(IO3)F (1), was discovered by introducing F atoms into alkaline-earth-metal iodates via a hydrothermal method. The pseudo-two-dimensional (2D) [BaF]+ layers and [IO3]- anionic groups built the crystal structure, which obviously exhibited large anisotropies. The birefringence of 1 was initially tested to be 0.1256 at 589.3 nm. Besides, compared with most reported metal iodate crystals, 1 possesses a large band gap (Eg = 4.32 eV). Theoretical calculations proved that the excellent optical characteristics can be attributed to [IO3]- anionic groups and [BaO4F4]10- polyhedra.

2.
Inorg Chem ; 2020 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-32401026

RESUMO

Two novel zinc borate halides named Zn2BO3X·H2O (X = Cl, Br) were discovered through hydrothermal techniques. Both compounds are isomorphic and feature the layered structure similar to that of KBBF, consisting of the infinite planar [Zn2BO3X·OH2] (X = Cl, Br) layers. Compared with the weak ionic bond between adjacent layers in KBBF, the strong interaction of hydrogen bonds between layers in Zn2BO3X·H2O (X = Cl, Br) effectively enhances the interlayer force, contributing to eliminating the layering growth tendency. Optical measurements on these two NLO crystals revealed that they have a broad wavelength transparency window and a moderate NLO coefficient. Moreover, the theoretical calculations revealed that the linear and NLO properties primarily depended on the [BO3] and [ZnO3Cl/Br] groups in both crystals.

3.
Molecules ; 25(8)2020 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-32316570

RESUMO

The ratio of substrate to catalyst (S/C) is a prime target for the application of asymmetric production of enantiomerically enriched intermediates by whole-cell biocatalyst. In the present study, an attractive increase in S/C was achieved in a natural deep-eutectic solvent (NADES) containing reaction system under microaerobic condition for high production of (S)-1-[3,5-bis(trifluoromethyl)phenyl]ethanol ((S)-3,5-BTPE) with Candida tropicalis 104. In PBS buffer (0.2 M, pH 8.0) at 200 rpm and 30 °C, 79.5 g (Dry Cell Weight, DCW)/L C. tropicalis 104 maintained the same yield of 73.7% for the bioreduction of 3,5-bis(trifluoromethyl)acetophenone (BTAP) under an oxygen-deficient environment compared with oxygen-sufficient conditions, while substrate load increased 4.0-fold (from 50 mM to 200 mM). Furthermore, when choline chloride:trehalose (ChCl:T, 1:1 molar ratio) was introduced into the reaction system for its versatility of increasing cell membrane permeability and declining BTAP cytotoxicity to biocatalyst, the yields were further increased to 86.2% under 200 mM BTAP, or 72.9% at 300 mM BTAP. After the optimization of various reaction parameters involved in the bioreduction, and the amount of biocatalyst and maltose co-substrate remained 79.5 g (DCW)/L and 50 g/L, the S/C for the reduction elevated 6.3 times (3.8 mM/g versus 0.6 mM/g). By altering the respiratory pattern of the whole-cell biocatalyst and exploiting the ChCl:T-containing reaction system, the developed strategy exhibits an attractive potential for enhancing catalytic efficiency of whole-cell-mediated reduction, and provides valuable insight for the development of whole-cell catalysis.

4.
RNA Biol ; : 1-11, 2020 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-32160106

RESUMO

Direct single-molecule sequencing of full-length transcripts allows efficient identification of gene isoforms, which is apt to alternative splicing (AS), polyadenylation, and long non-coding RNA analyses. However, the identification of gene isoforms and long non-coding RNAs with novel regulatory functions remains challenging, especially for species without a reference genome. Here, we present a comprehensive analysis of a combined long-read and short-read transcriptome sequencing in Camellia japonica. Through a novel bioinformatic pipeline of reverse-tracing the split-sites, we have uncovered 257,692 AS sites from 61,838 transcripts; and 13,068 AS isoforms have been validated by aligning the short reads. We have identified the tissue-specific AS isoforms along with 6,373 AS events that were found in all tissues. Furthermore, we have analysed the polyadenylation (polyA) patterns of transcripts, and found that the preference for polyA signals was different between the AS and non-AS transcripts. Moreover, we have predicted the phased small interfering RNA (phasiRNA) loci through integrative analyses of transcriptome and small RNA sequencing. We have shown that a newly evolved phasiRNA locus from lipoxygenases generated 12 consecutive 21 bp secondary RNAs, which were responsive to cold and heat stress in Camellia. Our studies of the isoform transcriptome provide insights into gene splicing and functions that may facilitate the mechanistic understanding of plants.

5.
Life Sci ; 252: 117589, 2020 Mar 24.
Artigo em Inglês | MEDLINE | ID: mdl-32220622

RESUMO

BACKGROUND: Renal fibrosis is the characteristic of all kinds of chronic kidney diseases (CKDs). Fascin-1 plays an important role in tumor development, but the roles of fascin-1 in renal fibrosis have not been studied. Here, we explored the role of fascin-1 in renal fibrosis and the potential mechanisms. METHODS: Kidney unilateral ureteral obstruction (UUO) mouse model was used as an in vivo model, and proximal tubule epithelial cell lines treated with TGF-ß1 were used as in vitro model of renal fibrosis. Cell transfection was performed to manipulate the expression of miR-200b/c, fascin-1 and CD44. Western blotting, qRT-PCR, immunohistochemistry or immunofluorescence assays were used to measure levels of miR-200b/c, fascin-1, CD44, and fibrosis and EMT-related markers. H&E and Masson stainings were used to examine the degree of injury and fibrosis in kidneys. Dual luciferase assay was used to examine the interaction between miR-200b/c family and fascin-1. RESULTS: Fascin-1 and CD44 levels were both significantly up-regulated while miR-200b/c family was reduced in models of renal fibrosis. Furthermore, overexpression of miR-200b/c family and inhibition of fascin-1 or CD44 ameliorated renal fibrosis through suppressing EMT process. Mechanistically, miR-200b/c family directly and negatively regulated the expression of fascin-1. Overexpression of fascin-1 could reverse the effects of miR-200b/c family on renal fibrosis, and fascin-1 regulated renal fibrosis by activating CD44. CONCLUSION: Our study is the first to show that fascin-1 plays a critical role in renal fibrosis. MiR-200b/c family could inhibit renal fibrosis through modulating EMT process by directly targeting fascin-1/CD44 axis.

6.
Artigo em Inglês | MEDLINE | ID: mdl-32101408

RESUMO

Thermoelectric (TE) materials have attracted extensive interest because of their ability to achieve direct heat-to-electricity conversion. They provide an appealing renewable energy source in a variety of applications by harvesting waste heat. The record-breaking figure of merit reported for single crystal SnSe has stimulated related research on its polycrystalline counterpart. Boosting the TE conversion efficiency requires increases in the power factor and decreases in thermal conductivity. It is still a big challenge, however, to optimize these parameters independently because of their complex interrelationships. Herein, we propose an innovative approach to decouple electrical and thermal transport by incorporating carbon fiber (CF) into polycrystalline SnSe. We show that the incorporation of highly conductive CF can successfully enhance the electrical conductivity, while greatly reducing the thermal conductivity of polycrystalline SnSe. As a result, a high TE figure-of-merit (zT) of 1.3 at 823 K is obtained in p-type SnSe/CF composite polycrystalline materials. Furthermore, SnSe samples incorporated with CFs exhibit superior mechanical properties, which are favorable for device fabrication applications. Our results indicate that the dispersion of CF can be a good way to greatly improve both TE and mechanical performance.

7.
Postgrad Med ; 132(2): 141-147, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31900004

RESUMO

Objective: To characterize the influence of relative wall thickness (RWT) on Cornell, Sokolow-Lyon and Peguero-Lo Presti voltages and elucidate its potential impacts on their diagnostic accuracy for LVH in a large general Chinese population.Methods: A total of 10,614 permanent residents aged ≥ 35 years were recruited for this study. All the participants were subjected to ECG and echocardiogram during the same visit. Multivariate linear and logistic regression analyzes were conducted to assess the influence of RWT on the voltages and their diagnostic performance for LVH detection.Results: A distinct correlation was identified between RWT and Cornell and Peguero-Lo Presti voltages following adjustments for age, gender and left ventricular mass (LVM) (ß = 0.675 and 1.342, respectively; Ps < 0.001). Besides, subjects with RWT > 0.42 exhibited higher rates of LVH diagnosed by Cornell (OR = 1.78, 95% CI: 1.45-2.20), Sokolow-Lyon (OR = 1.30, 95% CI: 1.08-1.56), and Peguero-Lo Presti voltage (OR = 1.48, 95% CI: 1.29-1.70) after adjustments for age, gender and echocardiographic LVH. Furthermore, concentric remodeling or concentric hypertrophy displayed higher rates of LVH diagnoses via Cornell and Peguero-Lo Presti voltage criteria, as compared with normal geometry or eccentric hypertrophy, respectively (all Ps < 0.05), findings of which were independent of age, gender and LVMI.Conclusion: Echocardiographic RWT was independently correlated with electrocardiographic voltage measures of LVH, which influenced their positive rates and contributed to poor diagnostic performance.


Assuntos
Ecocardiografia/métodos , Eletrocardiografia/métodos , Hipertrofia Ventricular Esquerda/diagnóstico , Hipertrofia Ventricular Esquerda/patologia , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , China , Feminino , Humanos , Hipertrofia Ventricular Esquerda/diagnóstico por imagem , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Fatores Sexuais
8.
Genomics ; 112(1): 286-288, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-30772429

RESUMO

Synteny and collinearity analysis is a standard investigative strategy done in many comparative genomic studies to understand genomic conservation and evolution. Currently, most visualization toolkits of synteny and collinearity do not emphasize the graphical representation of the results, especially the lack of extensible format on vector graphics outputs. This limitation becomes more apparent as 3rd generation sequencing brings high-throughput data, requiring relatively higher resolution for the resulting images. We developed VGSC2, the 2nd version of the web-based vector graph toolkit for genome synteny and collinearity analysis. The updated version enables four types of plots for synteny and collinearity, and three types of plots for gene family evolutionary research. Using web-based technologies, VGSC2 provides an easy-to-use user interface to display the homologous genomic result into vector graphs such as SVG, EPS, and PDF, as well as an online editor. VGSC2 is open source and freely available for use online through the web server available at http://bio.njfu.edu.cn/vgsc2.

9.
Neural Netw ; 122: 174-182, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31683145

RESUMO

Fine-grained image classification is a challenging task due to the large inter-class difference and small intra-class difference. In this paper, we propose a novel Cascade Attention Model using the Deep Convolutional Neural Network to address this problem. Our method first leverages the Spatial Confusion Attention to identify ambiguous areas of the input image. Two constraint loss functions are proposed: the Spatial Mask loss and the Spatial And loss; Second, the Cross-network Attention, applying different pre-train parameters to the two stream architecture. Also, two novel loss functions called Cross-network Similarity loss and Satisfied Rank loss are proposed to make the two-stream networks reinforce each other and get better results. Finally, the Network Fusion Attention merges intermediate results with the novel entropy add strategy to obtain the final predictions. All of these modules can work together and can be trained end to end. Besides, different from previous works, our model is fully weak-supervised and fully paralleled, which leads to easier generalization and faster computation. We obtain the state-of-the-art performance on three challenge benchmark datasets (CUB-200-2011, FGVC-Aircraft and Flower 102) with results of 90.8%, 92.1%, and 98.5%, respectively. The model will be publicly available at https://github.com/billzyx/LCA-CNN.

10.
Artigo em Inglês | MEDLINE | ID: mdl-31814234

RESUMO

An ammonium-containing metal iodate fluoride compound, (NH4 )Bi2 (IO3 )2 F5 , featuring a two-dimensional double-layered framework constructed by [BiO2 F5 ]6- and [BiO4 F4 ]9- polyhedra, as well as [IO3 ]- groups, was successfully synthesized. The well-ordered alignment of these SHG-active units leads to an extraordinary strong SHG response of 9.2 times that of KDP. Moreover, this compound possesses a large birefringence (Δn=0.0690 at 589.3 nm), a wide energy band gap (Eg =3.88 eV), and a high laser damage threshold (LDT; 40.2×AgGaS2 ). In particular, thermochromic behavior was observed for the first time in this type of compound. Such multifunctional crystals will expand the application of nonlinear optical materials.

11.
Opt Lett ; 44(23): 5659-5662, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31774747

RESUMO

We report on a narrowband, nonresonant periodically poled lithium niobate (PPLN) optical parametric oscillator using a volume Bragg grating (VBG) as the spectral narrowing element. Pumping by a Nd:YVO4 laser at 1.06 µm, a maximum output power of 4.75 W is achieved at a repetition rate of 20 kHz for a conversion efficiency of 47.5%. Both signal and idler spectra are narrowed to less than 2 nm, at good beam quality and stability.

12.
PeerJ ; 7: e8019, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31720123

RESUMO

MADS-box genes encode transcription factors that participate in various plant growth and development processes, particularly floral organogenesis. To date, MADS-box genes have been reported in many species, the completion of the sequence of the willow genome provides us with the opportunity to conduct a comprehensive analysis of the willow MADS-box gene family. Here, we identified 60 willow MADS-box genes using bioinformatics-based methods and classified them into 22 M-type (11 Mα, seven Mß and four Mγ) and 38 MIKC-type (32 MIKCc and six MIKC*) genes based on a phylogenetic analysis. Fifty-six of the 60 SsMADS genes were randomly distributed on 19 putative willow chromosomes. By combining gene structure analysis with evolutionary analysis, we found that the MIKC-type genes were more conserved and played a more important role in willow growth. Further study showed that the MIKC* type was a transition between the M-type and MIKC-type. Additionally, the number of MADS-box genes in gymnosperms was notably lower than that in angiosperms. Finally, the expression profiles of these willow MADS-box genes were analysed in five different tissues (root, stem, leave, bud and bark) and validated by RT-qPCR experiments. This study is the first genome-wide analysis of the willow MADS-box gene family, and the results establish a basis for further functional studies of willow MADS-box genes and serve as a reference for related studies of other woody plants.

14.
Oncol Rep ; 42(4): 1580-1588, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31364747

RESUMO

The objectives of the present study were to obtain the multigene mutation spectra of female breast cancer patients in Northeast China, to explore the correlation between mutations and clinicopathological characteristics, and to identify genetic mutations that correlate with the prognosis and survival of breast cancer patients. An Ion Torrent sequencing platform was used to detect mutations, including 31 known gene mutations associated with breast cancer, in 621 specimens from 286 breast cancer patients. A total of 286 patients were enrolled in this study. Eleven harmful/pathogenic gene mutations were found in 54.2% (155/286) of the patients, and 179 somatic nonsynonymous mutations were detected. Approximately 5.6% (16/286) of the patients carried two or more gene mutations. Among the 11 pathogenic gene mutations, those in PIK3CA were the most common and were detected in 65.4% (117/179) of the patients; TP53 gene mutations were the second most common and were detected in 20.7% (37/179) of the patients. Additional mutations were found in AKT (14/179; 7.8%) and PTEN (4/179; 2.2%), and mutations in the remaining 7 genes were each detected in approximately 0.6% (1/179) of the patients. Excluding 6 cases of breast ductal carcinoma in situ, the remaining 280 breast cancer cases were divided into four groups by molecular subtype, and the mutation frequencies of the 11 breast cancer­associated genes differed among the four groups. Furthermore, these 280 breast cancer cases were divided into two clinically relevant therapeutic groups: the HR+/HER2­ and triple­negative groups. The triple­negative group had a high frequency of TP53 mutations (21.8%) and a low frequency of PIK3CA mutations (21.8%), whereas the HR+/HER2­ group harbored TP53 mutations at a low frequency (10.1%) and PIK3CA mutations at a high frequency (50.0%). Cancerous, paracancerous, and normal tissues were collected from 72 patients and subjected to next­generation sequencing. The types and frequencies of somatic nonsynonymous mutations differed among the three studied tissue types, reflecting the genetic heterogeneity of different tissues from the same individual. In addition, tissues from 70 patients (excluding 2 patients with ductal carcinoma in situ) were divided into four groups according to molecular subtype, and the gene mutation frequencies in cancerous, paracancerous, and normal tissues differed among the four groups. After normalization, gene mutations were detected at a higher rate in cancerous tissues than in paracancerous and normal tissues in all groups, except for the HER2­positive group (which had a small sample size). In addition, Cox multivariate analyses of clinicopathological data, gene sequencing results, and 5­year survival rates of the 286 patients showed that gene mutations in the PTEN­PI3K/AKT signaling pathway were independently associated with a poor prognosis (P<0.05). In conclusion, mutations in the PTEN­PI3K/AKT signaling pathway may be valuable in the prediction of the prognosis and survival of breast cancer patients.


Assuntos
Neoplasias da Mama/genética , Família Multigênica , Mutação , Adulto , Neoplasias da Mama/enzimologia , China , Análise Mutacional de DNA , DNA de Neoplasias/sangue , DNA de Neoplasias/genética , Feminino , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Humanos , Pessoa de Meia-Idade , PTEN Fosfo-Hidrolase/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Lesões Pré-Cancerosas/enzimologia , Lesões Pré-Cancerosas/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais
15.
Chem Commun (Camb) ; 55(74): 11139-11142, 2019 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-31463508

RESUMO

Two unprecedented metal borate-iodates, Sr[B(OH)4](IO3) (SBIO) and Li4Sr5[B12O22(OH)4](IO3)2 (LSBIO), were synthesized via the strategy of introducing (IO3)- into borates. In particular, the LSBIO possessed a new B12O26 fundamental building block (FBB). The two compounds integrated the advantages of both pure iodates and borates, showing enlarged band gaps and birefringence. Moreover, diverse B-O FBBs achieved the modulation of birefringence. This work reported the first two examples of borate-iodates.

16.
Exp Ther Med ; 18(3): 1961-1966, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31452696

RESUMO

Regulatory effect of Toll-like receptor 4 (TLR4) gene on periodontitis in mice was investigated to explore its possible mechanism. Thirty C57/BL6 mice were randomly divided into the blank control group (N group, n=10), the periodontitis group (P group, n=10) and the periodontitis + TAK-242 group (PT group, n=10). The mice in P and PT group were ligatured with silk threads dipped with porphyromonas gingivalis (P. gingivalis) in the logarithmic phase to induce experimental periodontitis, and TAK-242 was intraperitoneally injected on the day when the periodontitis model was established. After fasting for 8 h, the expression levels of high-sensitivity C-reactive protein and inflammatory cytokines were measured in each group of mice. Their alveolar bones were isolated and changes were detected. Quantitative polymerase chain reaction was used to detect the expression levels of TLR4. After the mice were given TAK-242, the levels of hs-CPR, MCP-1, IL-6 and IL-1ß in the PT group evidently increased (P<0.01) compared with those in the N group. After the mice were administered TAK-242, the alveolar bone density, the percentage of bone volume and the number of bone trabeculae in PT group were significantly reduced, and the bone trabecular space and structural model index were evidently decreased (P<0.01). In addition, the expression levels of and T-bet/GATA3 messenger ribonucleic acids (mRNAs) in peria of mice in the P group were significantly higher than those in the N group (P<0.01), whereas the expression level of Foxp3 mRNA was notably decreased (P<0.01). The involvement of TLR4 gene in the inflammatory response of periodontitis results in periodontitis, and its mechanism may be that it activates TLR4, so as to affect the expression of T-bet, GATA3 and Foxp3.

17.
Biomed Res Int ; 2019: 2150291, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31275966

RESUMO

[This corrects the article DOI: 10.1155/2016/7823429.].

18.
BMJ Open ; 9(7): e029463, 2019 07 02.
Artigo em Inglês | MEDLINE | ID: mdl-31270122

RESUMO

OBJECTIVES: This current study was performed to characterise the independent associations of obesity and hypertension with interatrial block (IAB) after adjusting for cardiovascular risk factors, echocardiographic left atrial diameter (LAD) and left ventricular mass index (LVMI) in a large general Chinese population. DESIGN: A cross-sectional study. SETTING AND PARTICIPANTS: A total of 11 956 permanent residents (≥35 years of age) from Liaoning Province in China were included in this study. Following the completion of a questionnaire, the enrolled participants were subjected to physical examinations, laboratory analyses, ECG and echocardiogram. Linear and logistic regression analyses were performed to evaluate the associations of hypertension and obesity with IAB. OUTCOME MEASURES: IAB was defined as a prolongation of the P wave duration ≥120 ms on a digital 12-lead ECG. RESULTS: The prevalence of IAB in hypertensive individuals was higher than the normotensive in both men (9.5 vs 5.9%; p<0.001) and women (6.6 vs 3.6%; p<0.001). In addition, the prevalence of IAB exhibited a sharp increase with advancing body mass index (BMI) in both men (from 4.9% to 13.0%) and women (from 3.5% to 6.9%) (ps- for trend <0.001). Multiple relevant clinical covariates, echocardiographic LAD and LVMI were adjusted in the multivariate linear and logistic regression analyses. The results revealed that systolic blood pressure, diastolic blood pressure and BMI were all independently associated with P wave duration (ß=0.02, 0.09 and 0.25, respectively; all ps <0.005). Furthermore, hypertension was found to be independently associated with IAB (OR=1.27; p=0.018), while both overweight and obesity exhibited higher odds of IAB (OR=1.42 and 1.67, respectively; ps <0.005), compared with BMI <24.0 kg/m2. CONCLUSIONS: The key findings of this study highlighted that hypertension and overweight/obesity were independently and significantly associated with IAB in general Chinese population.

19.
Biomed Res Int ; 2019: 1656123, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31360702

RESUMO

Aims: We performed the current study primarily to characterize the independent association of blood pressure with heart rate-corrected QT (QTc) interval after adjusting for cardiovascular confounding factors and left ventricular mass (LVM) in a large general population in China. Methods: All enrolled 10,553 permanent residents with age ≥ 35 years from Liaoning Province were investigated by a questionnaire and then subjected to physical examinations, laboratory analyses, and electrocardiogram (ECG) as well as echocardiogram at the same visit. Multivariate linear and logistic regression analyses were conducted to assess the independent association of blood pressure with QTc interval. Results: Hypertensive subjects had significantly longer QTc interval and higher prevalence of prolonged QTc interval compared with normotensive ones in all subgroups stratified by gender and left ventricular hypertrophy (LVH) (all Ps ≤ 0.001). Multiple relevant clinical confounding factors and LVM were all adjusted in the multivariate linear and logistic regression analyses. As a result, both systolic blood pressure (SBP) and diastolic blood pressure (DBP) were independently associated with QTc interval (ß = 0.12 and 0.16, respectively; Ps < 0.001). Furthermore, as categorical variables, hypertension was independently associated with prolonged QTc interval (OR = 1.71; P < 0.001). Sex-specific analyses revealed that the independent associations were detected in both males and females (all Ps < 0.001). Conclusions: These key findings of the current study highlighted the fact that hypertension was significantly associated with prolonged QTc interval and the correlations were independent of confounding factors and LVM.


Assuntos
Eletrocardiografia , Frequência Cardíaca , Hipertensão , Hipertrofia Ventricular Esquerda , Adulto , Idoso , Pressão Sanguínea , China/epidemiologia , Feminino , Humanos , Hipertensão/epidemiologia , Hipertensão/fisiopatologia , Hipertrofia Ventricular Esquerda/epidemiologia , Hipertrofia Ventricular Esquerda/fisiopatologia , Masculino , Pessoa de Meia-Idade , Prevalência , Fatores Sexuais
20.
Biomed Environ Sci ; 32(7): 531-540, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31331437

RESUMO

OBJECTIVE: To evaluate the effect of intranasal immunization with CTA1-DD as mucosal adjuvant combined with H3N2 split vaccine. METHODS: Mice were immunized intranasally with PBS (negative control), or H3N2 split vaccine (3 µg/mouse) alone, or CTA1-DD (5 µg/mouse) alone, or H3N2 split vaccine (3 µg/mouse) plus CTA1-DD (5 µg/mouse). Positive control mice were immunized intramuscularly with H3N2 split vaccine (3 µg/mouse) and alum adjuvant. All the mice were immunized twice, two weeks apart. Then sera and mucosal lavages were collected. The specific HI titers, IgM, IgG, IgA, and IgG subtypes were examined by ELISA. IFN-γ and IL-4 were test by ELISpot. In addition, two weeks after the last immunization, surivival after H3N2 virus lethal challenge was measured. RESULTS: H3N2 split vaccine formulated with CTA1-DD could elicit higher IgM, IgG and hemagglutination inhibition titers in sera. Furthermore, using CTA1-DD as adjuvant significantly improved mucosal secretory IgA titers in bronchoalveolar lavages and vaginal lavages. Meanwhile this mucosal adjuvant could enhance Th-1-type responses and induce protective hemagglutination inhibition titers. Notably, the addition of CTA1-DD to split vaccine provided 100% protection against lethal infection by the H3N2 virus. CONCLUSION: CTA1-DD could promote mucosal, humoral and cell-mediated immune responses, which supports the further development of CTA1-DD as a mucosal adjuvant for mucosal vaccines.


Assuntos
Adjuvantes Imunológicos , Toxina da Cólera , Vírus da Influenza A Subtipo H3N2/imunologia , Vacinas contra Influenza , Proteínas Recombinantes de Fusão , Administração Intranasal , Animais , Feminino , Imunidade Humoral , Camundongos Endogâmicos BALB C , Mucosa Nasal/imunologia , Distribuição Aleatória
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