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2.
Artigo em Chinês | MEDLINE | ID: mdl-32062902

RESUMO

Pulmonary fibrosis is the terminal manifestation of a variety of interstitial lung diseases. Idiopathic pulmonary fibrosis (IPF) is one of the chronic, progressive, fibrotic lung disease with high incidence and poor prognosis. Nintedanib and pirfenidone are currently marketed anti-pulmonary fibrosis drugs, and their efficacy and safety are recognized in patients with IPF. This article reviews the targets and clinical trials of the two drugs, and provides a basis for the expansion of indications for anti-pulmonary fibrosis drugs.


Assuntos
Indóis/farmacologia , Piridonas/farmacologia , Ensaios Clínicos Controlados Aleatórios como Assunto , Humanos
3.
Zhonghua Gan Zang Bing Za Zhi ; 28(1): 53-57, 2020 Jan 20.
Artigo em Chinês | MEDLINE | ID: mdl-32023700

RESUMO

Objective: To study the occurrence of sarcopenia in patients with liver cirrhosis, and to explore their risk factors and impact on clinical outcomes. Methods: 199 hospitalized cases with liver cirrhosis were collected for nutritional risk screening, anthropometric measurement and blood biochemical examination. The body composition analysis was measured based on the skeletal muscle content of the four limbs to calculate the appendicular skeletal muscle mass index (ASMI). Patients were divided into sarcopenia and non-sarcopenia group and the relevant indexes of both groups were compared to screen for factors affecting the occurrence of sarcopenia. During the follow-up of 48 months, the survival and complications of the both groups were compared. Statistical analysis was performed using t-test, χ(2) test and logistic regression analysis in terms of different data. Results: The incidence of sarcopenia in cirrhosis was 36.7%, with the highest prevalence in patients with recurrent hepatic encephalopathy (62.5%), followed by patients with abdominal ascites / pleural effusion (37.6%). The incidence of sarcopenia was significantly higher in those with nutritional risk than in those without nutritional risk (P < 0.05). However, even among those without nutritional risk, 14.8% had combined sarcopenia. The body mass index (BMI), upper arm muscle circumference (AMC), and body cell mass (BCM) of the sarcopenia group were lower than those of the non-sarcopenia group (P < 0.05), and the edema index (ECW/TBW) was higher than the latter (P < 0.05). Multivariate analysis showed that age, gender, BMI, and complications of hepatic encephalopathy were the main influencing factors of cirrhosis combined with sarcopenia (P < 0.05). During the follow-up period, the sarcopenia group had a higher mortality rate than non-sarcopenia goup (P < 0.05), and the incidence of recurrent abdominal ascites/pleural effusion, hepatic encephalopathy, and infection was also significantly elevated (P < 0.05). Conclusion: Sarcopenia is one of the manifestations of malnutrition in patients with liver cirrhosis, which increases the risk of mortality and other complications, and has adverse impact on the clinical outcome. Additionally, older age, male sex, low BMI and recurrent hepatic encephalopathy has higher risk for developing sarcopenia.


Assuntos
Cirrose Hepática , Sarcopenia , Idoso , Encefalopatia Hepática , Humanos , Masculino , Músculo Esquelético , Fatores de Risco
4.
Zhonghua Yi Xue Za Zhi ; 100(4): 301-306, 2020 Feb 04.
Artigo em Chinês | MEDLINE | ID: mdl-32075360

RESUMO

Objective: To investigate the clinicopathological characteristics, MSI and K-ras mutation of double primary malignancies (DPM) associated with colorectal cancer (CRC). Methods: From January 2015 to December 2016, the clinicopathological data of CRC patients treated by surgery in the Affiliated Drum Tower Hospital of Nanjing University Medical School were collected, and the clinical data was analyzed. Multiplex real-time fluorescence quantitative PCR and amplification refractory mutation was performed to identify MSI and K-ras gene mutations. Results: Of all patients with CRC, 5.2% (55/1 066) were DPM. There was no significant difference in the male and female ratio, age, colorectal cancer site, T stage, N stage composition ratio between DPM patients with CRC and patients with single CRC (P>0.05). There were significant difference of TNM stage between the two group (P<0.05). The most frequent location of CRC was the colon in both DPM patients with CRC and patients with single CRC[35.5% (359/1 011) and 41.8% (23/55), respectively]. Of 55 DPM patients with CRC, 48 were metachronous DPM patients, 7 were synchronous DPM patients and 41 were colorectal cancer first. In extracolonic organ, digestive system (23/55) was the most commonly occurring system and stomach (11/55) was the most common lesion. DPM patients with CRC had higher incidence of MSI-H than patients with single CRC (P<0.05). There was no significant difference of K-ras gene mutation between DPM patients with CRC and patients with single CRC (P>0.05). MSI-H and K-ras mutation were present in only 2 patients of DPM patients with CRC. Conclusions: The rectum is the most common lesion site in CRC patients. The stomach is the most common extracolonic organ of DPM patients with CRC. DPM patients with CRC has high risk of MSI-H, but no significant difference in the incidence of K-ras mutation.


Assuntos
Neoplasias Colorretais , Genes ras , Proteínas Proto-Oncogênicas p21(ras)/genética , Neoplasias Colorretais/genética , Feminino , Humanos , Masculino , Instabilidade de Microssatélites , Mutação , Estadiamento de Neoplasias
5.
Bioresour Technol ; 301: 122798, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31981907

RESUMO

The polyvinyl formal (PVFM) biocarrier addition in a membrane bioreactor (MBR) was evaluated at high and low carbon/nitrogen (C/N) ratio of 20.0 and 6.7. Results indicated that static biocarrier addition could enrich nitrification and denitrification bacteria, dominating by Tauera, Amaricoccus and Nitrosospira at the genus level and slightly improved the total nitrogen removal even at a low C/N ratio. The bulk sludge characteristics (such as bigger particle size, lower SMP, lower SMP P/C) were also significantly changed in the hybrid MBR (HMBR), leading to a more sustainable membrane operation. The biocarrier addition also reduced the relative abundance of Sphingobacterials_unclassified, Ohtaekwangia and Rhodocyclaceae_unclassified at the genus level, indicating less membrane fouling in the HMBR. Consequently, HMBR with static PVFM addition could partially overcome the drawback of low C/N ratio for total nitrogen removal and membrane fouling control, providing a more resilient MBR to the undesirable environment such as low C/N ratio.


Assuntos
Microbiota , Nitrogênio , Reatores Biológicos , Desnitrificação , Membranas Artificiais , Esgotos , Eliminação de Resíduos Líquidos
6.
Public Health ; 181: 34-39, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31935609

RESUMO

OBJECTIVES: The interest in built environment attributes on physical activity has been rapidly increasing over recent decades. This study aimed to investigate the associations between residential density (RD) and walking time (WT), a common mode of physical activity, among urban adults in China. STUDY DESIGN: This is a cross-sectional study. METHODS: Locally registered adults aged 35-74 years were randomly selected from eight urban neighbourhoods in Nanjing, China, in 2017. The outcome variable was self-reported WT (dichotomised), whereas RD (in tertiles) was the independent variable. Mixed-effects regression models were introduced to examine the RD-WT association via computing odds ratios (ORs) and 95% confidence intervals (CIs). RESULTS: Of 1568 eligible participants, 98.9% completed the survey, with a mean age (standard deviation) of 54.7 (11.1) years, in which 46% were men. After adjustment for potential confounders, a negative gradient RD-WT association was seen across RD tertiles among the participants (OR = 2.88 [95% CI = 1.79, 4.64] and 2.10 [95% CI = 1.35, 3.26] for lower and middle tertiles, respectively, compared with the upper RD tertile). The negative RD-WT association was observed for both men and women. CONCLUSIONS: RD was negatively associated with the WT of urban adults in China. These results have public health implications for improving community-level physical activity through development of walking-friendly built environment.

7.
Plant Biol (Stuttg) ; 22(2): 212-220, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31627255

RESUMO

Leaf venations have elements with relatively lower elasticity than other leaf tissue components, which are thought to contribute to leaf biomechanics. A better mechanistic understanding of relationships between vein traits and leaf mechanical properties is essential for ecologically relevant interpretation of leaf structural variations. We investigated 13 major (first to third order) and minor (>third order) vein traits, six leaf mechanical properties and other structural traits across 58 woody species from a subtropical forest to elucidate how vein traits contribute to leaf biomechanics. Across species, vein dry mass density (ρv ), total vein dry mass per leaf area (VMA) and minor vein diameter (VDmin ), but not the lower-order vein density (VLA1•2 ), were positively correlated with leaf force to punch (Fp ) and force to tear (Ft ). Structural equation models showed that ρv and VDmin not only contribute to leaf mechanical properties directly (direct pathway), but also had impacts on leaf biomechanics by influencing leaf thickness and leaf dry mass per area (indirect pathway). Our study demonstrated that vein dry mass density and minor vein diameter are the key vein properties for leaf biomechanics. We also suggest that the mechanical characteristics of venations are potential factors influencing leaf mechanical resistance, structure and leaf economics spectrum.

8.
Biotech Histochem ; 95(5): 325-332, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31850810

RESUMO

Lyophilization is a practical method for product storage and transportation; it commonly is used in the food and pharmaceutical industries. Lyophilization also is used for preserving biological samples such as serum, plasma and animal tissues. We found that lyophilization does not affect the stability of RNAs and proteins in tissue samples. To investigate histological characteristics, we prepared lyophilized tissues for paraffin sectioning and hematoxylin and eosin (H & E) staining. We also measured water loss from organs during lyophilization. We used immunohistochemistry of frozen brain sections to identify potential protective effects of three concentrations of sucrose, glucose and trehalose against the effects of lyophilization. H & E staining revealed vacuoles in heart, lung, liver, kidney, spleen and brain after lyophilization without pretreatments, especially heart and kidney. We found that 10% solutions of sucrose, glucose and trehalose helped preserve tissue morphology. Immunohistochemistry of frozen brain tissue showed that 10% glucose and 30% sucrose preserved cellular characteristics and immunogenicity following lyophilization. Lyophilization removed > 70% of the water from organs, and lyophilized tissues without protectants were not suitable for histological study. Overall, we found that 10% glucose helped preserve both optimal tissue morphology and immunogenicity of freeze-dried tissue.

9.
Niger J Clin Pract ; 22(12): 1772-1777, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31793487

RESUMO

Background: There are various endoscopic techniques for thyroid carcinoma dissection but few reports regarding the scarless neck technique and central compartment dissection (CCD) via the breast approach, especially for bilateral CCD are available. In this study, we reported 45 cases with scarless neck endoscopic total or near-total thyroidectomy plus CCD via the breast approach. Materials and Methods: Forty-five female patients with papillary thyroid carcinoma (PTC) were enrolled in the study, from January 2011 to March 2013. In brief, 5 mm ultrasonic coagulation device (Harmonic Scalpel, HS; Ethicon Endosurgery, USA) was used to perform thyroid vessel management and thyroidectomy. Twenty patients underwent total thyroidectomy and 25 underwent near-total thyroidectomy. CCD was performed in all 45 patients, including 13 with bilateral CCD and 32 with ipsilateral CCD. Results: The procedure was successful for all 45 patients. Sixteen patients (35.6%) had lymph node metastases in central compartments. Postoperative transient adverse events included voice changes (9 patients) and hypocalcemia (18 patients), including 7 (21.9%) in the unilateral group and 11 (84.6%) in bilateral group. There was no permanent hypocalcemia or recurrent laryngeal nerve (RLN) palsy. None of the case were converted to open surgical procedure. All patients were satisfied with the cosmetic result of the scarless neck endoscopic thyroidectomy (SET). No evidence of residual or recurrent disease was found during a mean follow-up of 22.84 months (range, 12-34 months). Conclusions: Experienced thyroid surgeons performed the scarless neck endoscopic total or near-total thyroidectomy plus unilateral or bilateral CCD via the breast approach for selected PTC patients. The procedure was safe and feasible with excellent cosmetic results.


Assuntos
Mama/cirurgia , Carcinoma Papilar/cirurgia , Endoscopia/métodos , Esvaziamento Cervical/métodos , Câncer Papilífero da Tireoide/cirurgia , Tireoidectomia/métodos , Adulto , Carcinoma Papilar/patologia , Feminino , Humanos , Metástase Linfática , Masculino , Pessoa de Meia-Idade , Câncer Papilífero da Tireoide/patologia , Resultado do Tratamento
10.
Zhonghua Yu Fang Yi Xue Za Zhi ; 53(10): 1059-1062, 2019 Oct 06.
Artigo em Chinês | MEDLINE | ID: mdl-31607056

RESUMO

Research participants were recruited at 16 elderly activity centers among three cities in Macao, Hong Kong and Guangzhou. A total of 324 people were surveyed, with women ≥ 55 years old and men ≥ 60 years old; There were 90, 100 and 134 in Macao, Hong Kong and Guangzhou respectively. The median (P(25), P(75)) score of the social support rating scale (SSRS) for the elderly was 30 (25, 38) for Macao, 24 (20, 29) for Hong Kong and 30 (26, 37) for Guangzhou respectively. The elderly in Macao and Guangzhou had a higher proportion of the SSRS in the middle and high groups (85.20%, 84.50%) when compared to the elderly in Hong Kong (62.10%) (P<0.05). The city, marital status, and personal well-being index are related to SSRS scores.


Assuntos
Satisfação Pessoal , Apoio Social , Idoso , China , Cidades , Feminino , Hong Kong , Humanos , Macau , Masculino , Pessoa de Meia-Idade
11.
Artigo em Chinês | MEDLINE | ID: mdl-31434382

RESUMO

Draf type Ⅱb/Ⅲ frontal sinus surgery has been widely used in clinical practice. They can obtain a wide operating space and sufficient drainage pathways. However, the mucosa around the frontal sinus ostium was removed during the surgery, resulting in bone exposure, and sinus ostium stenosis or atresia may occur after the operation. In recent years, rhinologists at home and abroad have applied various mucosal flaps to cover the exposed bone around the frontal sinus orifice after Draf Ⅱb/Ⅲ frontal sinus surgery, in order to reduce scar formation and bone hyperplasia along with the incidence of frontal sinus ostium stenosis or atresia after surgery. Satisfactory results have been achieved. The article reviews the application of mucosal flap technique in Draf Ⅱb/Ⅲ frontal sinus surgery, in order to promote the clinical research and technical development of this technique in China.


Assuntos
Seio Frontal/cirurgia , Membrana Mucosa/transplante , Doenças dos Seios Paranasais/cirurgia , Retalhos Cirúrgicos , China , Endoscopia , Humanos , Neoplasias da Base do Crânio/cirurgia , Resultado do Tratamento
12.
Eur Rev Med Pharmacol Sci ; 23(10): 4313-4320, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31173304

RESUMO

OBJECTIVE: Exercise-based intervention promises to be more effective in a structured framework for individuals with autism spectrum disorders (ASD). The aim of this study was to observe changes in behavior of individuals with ASD by investigating their physical status after the structured exercise-based intervention. PATIENTS AND METHODS: The exercise intervention integrated an 8-week exercise program that included aerobic, resistive, and neuromuscular exercises. Body composition and the Autism Treatment Evaluation Checklist (ATEC) were evaluated to assess changes after the exercise-based intervention. RESULTS: After the exercise intervention, the fat mass of individuals with ASD were significantly reduced, and their behavior improved markedly. CONCLUSIONS: This pilot study demonstrated that individuals with ASD require long-term, structured exercise-based intervention, and that such exercise-based intervention is effective for improving their health.

13.
Artigo em Chinês | MEDLINE | ID: mdl-31177691

RESUMO

Objective: To investigate the etiology, clinical features, treatment and outcome of nephrotic syndrome associated with chronic mercury poisoning. Methods: From June 2013 to April 2018, Beijing Chaoyang Hospital, Capital Medical University received 33 patients with chronic mercury-neutral nephrotic syndrome. The clinical manifestations, laboratory tests, treatment methods, and outcomes were analyzed. Results: Among the 33 patients, 27 patients had mercury exposure due to daily-life contact and the other 6 patients were caused by iatrogenic mercury. The symptom was characterized by typical nephrotic syndrome such as lower extremity edema and proteinuria at first onset. The treatment was based on mercury-removing treatment, 19 cases were treated with mercury removal alone, 16 cases were completely relieved; 10 cases were treated with mercury removal and glucocorticoids, all of which were completely relieved; 4 cases were treated with mercury removal, glucocorticoids and immunosuppressive agents, all complete remission; clinical complete remission rate is about 90.9% (30 cases in total) . Urinary mercury levels decreased the fastest between the first and second courses of mercury treatment, but the total amount of urine protein increased. As the amount of urinary mercury excreted increased, the total amount of urine protein decreased gradually (Z=2.86, P<0.01) . Conclusion: The clinical features of chronic mercury-induced nephrotic syndrome are non-specific, easy to be misdiagnosed and missed. The treatment is mainly treated with mercury removal treatment. The prognosis is good. In severe cases, glucocorticoid therapy can be supplemented.


Assuntos
Intoxicação por Mercúrio , Síndrome Nefrótica , Humanos , Intoxicação por Mercúrio/complicações , Síndrome Nefrótica/induzido quimicamente , Síndrome Nefrótica/terapia , Proteinúria , Resultado do Tratamento
14.
Transplant Proc ; 51(3): 823-832, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30979471

RESUMO

OBJECTIVE: To assess biliary and vascular complications after liver transplantations (LTs) sourced from deceased donors. METHODS: This study reviewed potentially relevant English-language articles gathered from PubMed and Medline published from 2012 to 2017. One additional study was carried out using our institution's database for articles published from 2013 to 2017. Biliary and vascular complications from adult patients receiving their first deceased-donor LT were included. This meta-analysis was performed using Review Manager version 5.2 (Cochrane Collaboration, Copenhagen, Denmark) and the study quality was evaluated using the Newcastle-Ottawa Scale. RESULTS: Ten studies met our inclusion criteria. Heterogeneity in donation after cardiac death (DCD) and donation after brain death (DBD) recipients was observed and minimized after pooling a subgroup analysis. This latter analysis focused on biliary stricture, biliary leaks and stones, and vascular thrombosis and stenosis. Meta-analyses showed that patients receiving DCD organs have a greatly increased risk of biliary complications compared to those receiving DBD organs, particularly the following: biliary leaks and stones (odds ratio [OR] = 1.69, 95% confidence interval [CI] 1.22-2.34); and biliary stricture (OR = 1.58, 95% CI 1.21-2.06). DCD grafts tended to be but were not significantly associated with DBD regarding vascular thrombosis (OR = 1.62, 95% CI 1.05-2.50), and the risk of vascular stenosis in DCD grafts was not statistically significant (OR = 1.25, 95% CI, .70-2.25). CONCLUSION: DCD was associated with an increased risk of biliary complications after LT, tended to indicate an increased risk of vascular thrombosis versus, and was not associated with an increased risk of vascular stenosis compared to DBD. There was no significant difference between the grafts.


Assuntos
Doenças Biliares/etiologia , Transplante de Fígado/efeitos adversos , Complicações Pós-Operatórias/epidemiologia , Doadores de Tecidos , Doenças Vasculares/etiologia , Adulto , Doenças Biliares/epidemiologia , Feminino , Sobrevivência de Enxerto , Humanos , Masculino , Pessoa de Meia-Idade , Razão de Chances , Doenças Vasculares/epidemiologia
15.
J Appl Microbiol ; 126(3): 740-751, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30556937

RESUMO

AIMS: To investigate the effects of the ginsenoside Rh2 on monospecies and multispecies cariogenic biofilms and explore the mechanism of the antibiofilm effect of Rh2 in vitro. METHODS AND RESULTS: Streptococcus mutans, Streptococcus sobrinus and Streptococcus sanguinis were chosen to form the monospecies or multispecies biofilms. Crystal violet staining and laser scanning confocal microscopy were used to observe the effect of Rh2 on biofilms in vitro. Cytotoxicity was examined by the Cell Counting Kit-8. The effects of Rh2 on bacterial membranes were observed via transmission electron microscopy (TEM). The isobaric tags for relative and absolute quantification (iTRAQ) method were used to profile the common differentially expressed proteins. Gene expression was analysed by reverse transcription quantitative polymerase chain reaction. In general, the treatment of cariogenic biofilms with Rh2 significantly decreased biomass accumulation by inhibiting bacterial growth and extracellular polysaccharide synthesis without any cytotoxic effects. TEM imaging showed that Rh2 could disrupt the cell membranes of these bacteria. The iTRAQ results indicated that the levels of mannose-specific IIC/D and acetaldehyde/alcohol dehydrogenase were substantially down-regulated, while the mRNA expression of the corresponding genes were significantly changed. CONCLUSIONS: Our data revealed a potential application for Rh2 in the protection against dental caries via the inhibition of cariogenic biofilms. SIGNIFICANCE AND IMPACT OF THE STUDY: This study describes the first application of a ginsenoside against multispecies cariogenic biofilms. Rh2 may serve as an alternative agent to prevent dental caries by effectively modulating the pathogenic potentials of oral biofilms.


Assuntos
Antibacterianos/farmacologia , Biofilmes/efeitos dos fármacos , Ginsenosídeos/farmacologia , Streptococcus mutans/efeitos dos fármacos , Streptococcus sanguis/efeitos dos fármacos , Streptococcus sobrinus/efeitos dos fármacos , Cárie Dentária/microbiologia , Humanos , Streptococcus mutans/genética , Streptococcus mutans/fisiologia , Streptococcus sanguis/genética , Streptococcus sanguis/fisiologia , Streptococcus sobrinus/genética , Streptococcus sobrinus/fisiologia
16.
Transplant Proc ; 50(10): 3816-3821, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30577273

RESUMO

Mild hypothermia reduces the damage caused by hypoxia and oxidative stress, but how this happens is not very clear. Mice were anesthetized and their core body temperature was maintained at 38 ± 0.5°C and 32 ± 0.5°C. The renal artery and renal veins were blocked for 35 minutes and reperfusion was performed. Twenty-four hours later, serum was obtained to detect the concentrations of creatinine. The expression of CIRP, TRX, Bcl-2, and Bax were detected in tissue samples using Western blot. Apoptosis was measured using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling and the apoptosis rates were calculated. SOD and MDA were detected to determine the extent of oxidative damage in different groups. The concentration of creatinine in the NC group was 2.11 ± 0.39 mg/dL. Compared to the IR group, the concentration of creatinine decreased in MH+IR group and showed a significant statistical difference (8.74 ± 1.38 mg/dL vs 15.36 ± 2.13 mg/dL, P < .01); the apoptosis rate also decreased with statistical significance (15.02 ± 1.45% vs 37.02 ± 5.70%, P < .01). Compared to the IR group, the expression of CIRP, TRX, and the Bcl-2/Bax ratio significantly increased in the MH+IR group. The SOD activity in the MH+IR group increased (26.90 ± 4.41 U/mgprot vs 16.85 ± 2.41 U/mgprot, P < .05) and the MDA level decreased (0.76 ± 0.18 nmol/mgprot vs 1.37 ± 0.32 nmol/mgprot, P < .05) compared to those of the IR group. Mild hypothermia protects mice kidneys from ischemia-reperfusion damage by reducing oxidative stress injury and apoptosis.


Assuntos
Lesão Renal Aguda/prevenção & controle , Hipotermia Induzida/métodos , Traumatismo por Reperfusão/prevenção & controle , Animais , Apoptose , Modelos Animais de Doenças , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Estresse Oxidativo
17.
Zhonghua Yu Fang Yi Xue Za Zhi ; 52(10): 1050-1055, 2018 Oct 06.
Artigo em Chinês | MEDLINE | ID: mdl-30392326

RESUMO

Objective: The serotype screening of Shigella flexneri from 1934 to 1965 preserved by the National Center for Medical Culture Collections was carried out, and the molecular characteristics of the serotype conversion strains were studied. Methods: Serotyping of Shigella flexneri in this study was conducted by slide agglutination and multiplex PCR, respectively. The gtrⅡ gene sequence alignment and pulsed field gel electrophoresis typing were performed on the serotype conversion strains. Results: Among the 255 strains of Shigella flexneri preserved in CMCC (B) from 1934 to 1965, 79 were carrying gtrⅡ gene, of which 19 strains and 1 strain were agglutinated with the Y serotype and X serotype, respectively, and furthermore, the multiplex PCR assays results showed serotypes 2a and 2b, respectively, and the strains were considered to have serotype conversion. The 20 strains carrying the gtrⅡ gene showed multiple nucleotide mutations. Besides 3 strains of 3 amino acid mutations, the amino acid sequences of the other 17 strains showed a stop codon in advance, resulting in functional inactivation of gtrⅡ. PFGE analysis revealed that the similarity between the serotype Y strain carrying the gtrⅡ gene and the serotype 2a strain was 75.8%-100%, and the similarity between the serotype X strain carrying the gtrⅡ gene and the serotype 2b strain was 81.6%-100%. Conclusion: Mutations in the gtrⅡ gene are more complicated in serotype-transforming Shigella flexneri serotype Y or X strains. Molecular typing suggests that the serotype-transforming Shigella flexneri serotype Y or X strains may be derived from the Shigella flexneri serotype 2a or 2b, and advance the serotype conversion to 1949.


Assuntos
Sorogrupo , Shigella flexneri/genética , Sequência de Aminoácidos , Eletroforese em Gel de Campo Pulsado , Sorotipagem , Shigella flexneri/isolamento & purificação
19.
Zhonghua Gan Zang Bing Za Zhi ; 26(7): 524-529, 2018 Jul 20.
Artigo em Chinês | MEDLINE | ID: mdl-30317776

RESUMO

Objective: To study the nutritional status and energy metabolic characteristics of patients with nonalcoholic fatty liver disease (NAFLD), and to provide evidence for clinical evaluation and intervention. Methods: A total of 359 NAFLD patients diagnosed on ultrasound from June 2015 to March 2017 were selected as study subjects and divided into mild, moderate to severe fatty liver disease group and 50 healthy subjects as control group. The changes of ICW, ECW, body fat, skeletal muscle, protein and visceral fat area (VFA) of patients and controls were analyzed by using body composition analyzer. The energy metabolism index was measured by the oxidation rate of resting energy expenditure(REE), respiratory quotient (RQ), and the oxidation rates of the three nutrients (CHO %, FAT %, and PRO %). According to different types of data, non-parametric tests like Kruskal-Wallis or χ(2) were used for this analysis. Results: Compared with the mild fatty liver group and the control group, the moderate and severe fatty liver group the BMI, waist circumference, waist-hip ratio were significantly elevated (P-value < 0.001), and their serum alanine aminotransferase, triglyceride, total cholesterol, high-density lipoprotein, low-density lipoprotein, FBS levels were significantly increased (P value < 0.05). The Body composition analysis showed that there was no significant difference in skeletal muscle content between the three groups (P = 0.067). The ICW, ECW, protein, body fat content of moderate and severe fatty liver group were significantly higher than those of mild fatty liver group and control group (P < 0.01), but there was no significant difference between the mild fatty liver group and the control group. There was significant difference in the VFA between the three groups, while VFA in the moderate and severe fatty liver group was significantly increased. Metabolic results showed that the RQ of patients with moderate-severe fatty liver and mild fatty liver were 0.72 ± 0.08 and 0.78 ± 0.06, respectively, which were lower than those of the control group (0.80 ± 0.02), P = 0.004. Resting energy expenditure (REE) was not significantly different between moderate and severe fatty liver group and mild fatty liver group (P = 0.207), but both were significantly higher than those of the control group (P < 0.001). The percentages of CHO, FAT and PRO in moderate and severe fatty liver group were 19.49% ± 9.71%, 66.23% ± 12.54% and 14.22% ± 6.11% respectively. Compared with the control group, CHO % decreased, and FAT % increased. Conclusion: NAFLD patients have different extent of nutritional imbalance and energy metabolism disorders, the use of Body Composition analyzer and metabolic cart can comprehensively assess and monitor NAFLD patient's nutrition and energy metabolism status, to provide a basis for clinical intervention.


Assuntos
Metabolismo Energético , Hepatopatia Gordurosa não Alcoólica , Estado Nutricional , Índice de Massa Corporal , Humanos , Circunferência da Cintura
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