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2.
Artigo em Inglês | MEDLINE | ID: mdl-34379180

RESUMO

BACKGROUND AND PURPOSE: The WHO recently designated salivary gland lymphoepithelial carcinoma as a unique malignant tumor that most commonly occurs in the parotid gland. This is a rare cancer and there are few reports in the literature. Among 854 patients with parotid gland tumors who were admitted to our institution, we diagnosed 12 patients (1.41%) with parotid lymphoepithelial carcinoma. METHODS: Retrospective analysis of 12 patients with parotid lymphoepithelial carcinoma diagnosed by the Department of Pathology, Xiangya Hospital of Central South University. RESULTS: All 12 patients had unilateral parotid gland disease and 8 had cervical lymph node metastasis. Five patients received PCR testing for the Epstein-Barr virus and two were positive. All patients received surgical treatment, two received surgical resection alone, nine received surgery and postoperative radiotherapy and chemotherapy, and one received surgery and postoperative chemotherapy. The postoperative follow-up time ranged from 13 to 77 months. As of the last follow-up, eight patients were tumor-free, one patient was lost to follow-up, and three patients died. The main cause of death was local tumor recurrence and multiple metastases throughout the body. CONCLUSION: Parotid lymphoepithelial carcinoma is a malignant neoplasm characterized by proliferation, invasion, and inclusion of poorly differentiated or undifferentiated carcinoma, and a high rate of metastasis to ipsilateral cervical lymph nodes. The comprehensive treatment method consists of radical resection combined with postoperative radiotherapy and chemotherapy. After this comprehensive treatment, the 1-year, 3-year, and 5-year overall survival rates of our patients were 100%, 78.8%, and 39.4%.

3.
J Int Med Res ; 49(4): 3000605211008325, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33906532

RESUMO

OBJECTIVE: To detect the expression of FK506-binding protein 5 (FKBP5) in human papillary thyroid carcinoma (PTC) tissues, and explore its possible role in the progression of PTC. METHODS: FKBP5 expression levels were assessed in 115 PTC tissues and corresponding normal tissues by immunohistochemistry. We also examined the correlations between FKBP5 expression and clinicopathological factors and survival in 75 patients with PTC. The effects of FKBP5 on the proliferation and apoptosis of PTC cells were detected by colony-formation, MTT, and flow cytometry assays, respectively. We further investigated the effects of FKBP5 on tumor growth in mice. RESULTS: We revealed high expression levels of FKBP5 in human PTC tissues compared with normal tissues. Furthermore, high FKBP5 expression was associated with an increased incidence of intraglandular dissemination, and lower overall and progression-free survival. FKBP5 depletion remarkably suppressed the proliferation and induced apoptosis of PTC cells in vitro. FKBP5 further contributed to the growth of PTC tumors in mice. CONCLUSIONS: The results of this study demonstrated the potential involvement of FKBP5 in the progression of PTC, and confirmed FKBP5 as a novel therapeutic target for PTC treatment.


Assuntos
MicroRNAs , Proteínas de Ligação a Tacrolimo , Câncer Papilífero da Tireoide , Neoplasias da Glândula Tireoide , Animais , Apoptose , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Regulação Neoplásica da Expressão Gênica , Humanos , Camundongos , Proteínas de Ligação a Tacrolimo/genética , Câncer Papilífero da Tireoide/genética , Neoplasias da Glândula Tireoide/genética
5.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 37(2): 113-118, 2021 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-33504416

RESUMO

Objective To investigate the clinical significance of immune-related long non-coding RNAs (lncRNAs) and their potential role in guiding the treatment of prostate cancer. Methods lncRNAs of prostate cancer were obtained from TCGA database. The immune-related gene sets were downloaded from Molecular Signatures Database. Gene-lncRNA co-expression was confirmed by Pearson correlation analysis, and univariate Cox regression and selected operator regression (Lasso) were performed to identify important and immune-related lncRNAs. "gglot package" and "survival package" of R software were used to evaluate the correlation between the lncRNAs and clinical characteristics and the prognostic value of the lncRNAs. lnc2RNA database was used to analyze the difference of lncRNAs between normal prostate tissue and prostate cancer tissue. Starbase and David database were used to determine the predict function of lncRNAs in prostate cancer. Results AL162586.1, AC138028.4, SLC25A25-AS1, AC002553.1, AC004816.1, LINC00641 and AC027796.4 were key immune-related lncRNAs, and their expression was positively associated with N stage; the expression levels of AL162586.1 and SLC25A25-AS1 increased with higher T stage. The expression levels of SLC25A25-AS1 and LINC00641 were significantly different in tumor tissues from that of normal tissues. The GO enrichment showed that SLC25A25-AS1 was mainly distributed in membrane, had negative regulation of mRNA splicing via spliceosome and by a nucleotide binding. KEGG pathway enrichment showed that targeted genes were mainly involved in spliceosome pathway. Conclusion lncRNA has become a new research direction in prostate cancer and SLC25A25-AS1 may affect the prognosis of patients through splicing pathway.


Assuntos
Neoplasias da Próstata , RNA Longo não Codificante , Biologia Computacional , Regulação Neoplásica da Expressão Gênica , Humanos , Masculino , Prognóstico , Neoplasias da Próstata/genética , RNA Longo não Codificante/genética
6.
Aging (Albany NY) ; 12(24): 25256-25274, 2020 11 20.
Artigo em Inglês | MEDLINE | ID: mdl-33226370

RESUMO

In this meta-analysis, we systematically investigated the correlation between single nucleotide polymorphisms (SNPs) and pancreatic cancer (PC) risk. We searched PubMed, Network Science, EMBASE, Cochrane Library, China National Knowledge Infrastructure (CNKI), China Science and Technology Periodical Database (VIP), and Wanfang databases up to January 2020 for studies on PC risk-associated SNPs. We identified 45 case-control studies (36,360 PC patients and 54,752 non-cancer individuals) relating to investigations of 27 genes and 54 SNPs for this meta-analysis. Direct meta-analysis followed by network meta-analysis and Thakkinstian algorithm analysis showed that homozygous genetic models for CTLA-4 rs231775 (OR =0.326; 95% CI: 0.218-0.488) and VDR rs2228570 (OR = 1.976; 95% CI: 1.496-2.611) and additive gene model for TP53 rs9895829 (OR = 1.231; 95% CI: 1.143-1.326) were significantly associated with PC risk. TP53 rs9895829 was the most optimal SNP for diagnosing PC susceptibility with a false positive report probability < 0.2 at a stringent prior probability value of 0.00001. This systematic review and meta-analysis suggest that TP53 rs9895829, VDR rs2228570, and CTLA-4 rs231775 are significantly associated with PC risk. We also demonstrate that TP53 rs9895829 is a potential diagnostic biomarker for estimating PC risk.


Assuntos
Antígeno CTLA-4/genética , Predisposição Genética para Doença/genética , Neoplasias Pancreáticas/genética , Receptores de Calcitriol/genética , Proteína Supressora de Tumor p53/genética , Humanos , Metanálise em Rede , Polimorfismo de Nucleotídeo Único/genética
7.
Kaohsiung J Med Sci ; 36(12): 1021-1029, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32767492

RESUMO

Bladder cancer (BCa) is one of the most common malignancies with high morbidity and mortality worldwide. In recent years, it is of great importance to investigate the molecular etiology associated with of BCa. Abnormal spindle-like microcephaly associated gene (ASPM) is the human orthologous of the Drosophila abnormal spindle (asp) and the most commonly mutated gene of autosomal recessive primary microcephaly. ASPM is overexpressed in several types of cancer cell lines and affects the progression and development of multiple types of cancers. However, its possible role in BCa progression is still unclear. Herein, we demonstrated the possible involvement of ASPM in the progression of BCa. We noticed that high expression of ASPM was positively associated with the poor prognosis. Its knockdown could significantly inhibit the proliferation of BCa cells in vitro and in mice. Therefore, we thought ASPM could act as a promising therapeutic target for BCa.


Assuntos
Proteínas do Tecido Nervoso/metabolismo , Neoplasias da Bexiga Urinária/metabolismo , Neoplasias da Bexiga Urinária/patologia , Idoso , Animais , Carcinogênese/genética , Carcinogênese/patologia , Linhagem Celular Tumoral , Proliferação de Células/genética , Regulação para Baixo/genética , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Masculino , Camundongos Endogâmicos BALB C , Camundongos Nus , Pessoa de Meia-Idade , Músculos/patologia , Invasividade Neoplásica , Proteínas do Tecido Nervoso/genética , Prognóstico , RNA Interferente Pequeno/metabolismo , Neoplasias da Bexiga Urinária/genética
8.
Medicine (Baltimore) ; 99(29): e20677, 2020 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-32702817

RESUMO

BACKGROUND: Single nucleotide polymorphisms (SNPs) have been inconsistently associated with atrophic gastritis (AG) risk. This meta-analysis aimed to synthesize relevant data on SNPs associated with AG. METHODS: To identify all associated studies of SNPs and AG published, databases had been searched through January 2020 from the databases of PubMed, China National Knowledge Infrastructure (CNKI), Web of Science, Embase, the Chinese Science and Technology Periodical Database (VIP), Cochrane Library, and Wanfang databases. With the help of network meta-analysis and Thakkinstian algorithm, the best genetic model with the strongest correlation with AG was selected, the final result - matching to the noteworthy correlation - was obtained by referring to the false positive reporting rate (false positive report probability, FPRP). Based on STREGA's stated criteria, the methodological quality of the data we collected was valued. Both Stata 14.0 and GeMTC will be used for a comprehensive review of the system and will be used in our meta-analysis. RESULTS: This study will provide a high-quality evidence to find the SNP most associated with AG susceptibility and the best genetic model. CONCLUSIONS: This study will explore which SNP is most associated with AG susceptibility. REGISTRATION: INPLASY202050016.


Assuntos
Gastrite Atrófica/genética , Predisposição Genética para Doença/genética , Polimorfismo de Nucleotídeo Único/genética , Algoritmos , China/epidemiologia , Feminino , Gastrite Atrófica/patologia , Humanos , Masculino , Metanálise em Rede
9.
Medicine (Baltimore) ; 99(25): e20448, 2020 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-32569167

RESUMO

BACKGROUND: Single nucleotide polymorphisms (SNPs) have been inconsistently associated with gastric cancer (GC) risk. This meta-analysis aimed to synthesize relevant data on SNPs associated with GC. METHODS: Databases were searched to identify association studies of SNPs and GC published through January 2020 from the databases of PubMed, Web of Science, Embase, Cochrane Library, China National Knowledge Infrastructure, the Chinese Science and Technology Periodical Database, and Wan fang databases. Network meta-analysis and Thakkinstian algorithm were used to select the most appropriate genetic model, along with false positive report probability for noteworthy associations. The methodological quality of data was assessed based on the STrengthening the REporting of Genetic Association Studies statement Stata 14.0 will be used for systematic review and meta-analysis. RESULTS: This study will provide a high-quality evidence to find the SNP most associated with GC susceptibility and the best genetic model. CONCLUSIONS: This study will explore which SNP is most associated with GC susceptibility. REGISTRATION: INPLASY202040132.


Assuntos
Neoplasias Gástricas/genética , Predisposição Genética para Doença , Humanos , Metanálise como Assunto , Polimorfismo de Nucleotídeo Único , Risco , Revisões Sistemáticas como Assunto
10.
Medicine (Baltimore) ; 99(26): e20486, 2020 Jun 26.
Artigo em Inglês | MEDLINE | ID: mdl-32590731

RESUMO

BACKGROUND: Single nucleotide polymorphisms (SNPs) have been inconsistently associated with osteosarcoma (OS) risk. This meta-analysis aimed to synthesize relevant data on SNPs associated with OS. METHODS: Databases were searched to identify association studies of SNPs and OS published through January 2020 from the databases of PubMed, Web of Science, Embase, Cochrane Library, China National Knowledge Infrastructure, the Chinese Science and Technology Periodical Database, and Wan fang databases. Network meta-analysis and Thakkinstian algorithm were used to select the most appropriate genetic model, along with false positive report probability for noteworthy associations. The methodological quality of data was assessed based on the STrengthening the REporting of Genetic Association Studies statement Stata 14.0 will be used for systematic review and meta-analysis. RESULTS: This study will provide a high-quality evidence to find the SNP most associated with OS susceptibility and the best genetic model. CONCLUSIONS: This study will explore which SNP is most associated with OS susceptibility. REGISTRATION: INPLASY202040023.


Assuntos
Neoplasias Ósseas/genética , Osteossarcoma/genética , Polimorfismo de Nucleotídeo Único , Predisposição Genética para Doença , Humanos , Metanálise em Rede , Projetos de Pesquisa , Medição de Risco , Revisões Sistemáticas como Assunto
11.
Medicine (Baltimore) ; 99(24): e20345, 2020 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-32541456

RESUMO

BACKGROUND: Single nucleotide polymorphisms (SNPs) have been inconsistently associated with pancreatic cancer (PC) risk. This meta-analysis aimed to synthesize relevant data on SNPs associated with PC. METHODS: Databases were searched to identify association studies of SNPs and PC published through January 2020 from the databases of PubMed, Web of Science, Embase, Cochrane Library, China National Knowledge Infrastructure, the Chinese Science and Technology Periodical Database (VIP) and Wanfang databases. Network meta-analysis and Thakkinstian algorithm were used to select the most appropriate genetic model, along with false positive report probability (FPRP) for noteworthy associations. The methodological quality of data was assessed based on the STREGA statement Stata 14.0 will be used for systematic review and meta-analysis. RESULTS: This study will provide a high-quality evidence to find the SNP most associated with pancreatic cancer susceptibility and the best genetic model. CONCLUSIONS: This study will explore which SNP is most associated with pancreatic cancer susceptibility.Registration: INPLASY202040023.


Assuntos
Neoplasias Pancreáticas/epidemiologia , Neoplasias Pancreáticas/genética , Polimorfismo de Nucleotídeo Único/genética , Algoritmos , Estudos de Casos e Controles , China/epidemiologia , Reações Falso-Positivas , Feminino , Predisposição Genética para Doença , Humanos , Masculino , Metanálise em Rede , Neoplasias Pancreáticas/sangue , Neoplasias Pancreáticas/mortalidade , Risco , Sensibilidade e Especificidade
12.
Biosci Biotechnol Biochem ; 84(9): 1788-1798, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32448038

RESUMO

Many phenolic compounds, derived from lignin during the pretreatment of lignocellulosic biomass, could obviously inhibit the activity of cellulolytic and hemicellulolytic enzymes. Acetosyringone (AS) is one of the phenolic compounds produced from lignin degradation. In this study, we investigated the inhibitory effects of AS on xylanase activity through kinetic experiments. The results showed that AS could obviously inhibit the activity of xylanase in a reversible and noncompetitive binding manner (up to 50% activity loss). Inhibitory kinetics and constants of xylanase on AS were conducted by the HCH-1 model (ß = 0.0090 ± 0.0009 mM-1). Furthermore, intrinsic and 8-anilino-1-naphthalenesulfonic (ANS)-binding fluorescence results showed that the tertiary structure of AS-mediated xylanase was altered. These findings provide new insights into the role of AS in xylanase activity. Our results also suggest that AS was an inhibitor of xylanase and targeting AS was a potential strategy to increase xylose production.


Assuntos
Acetofenonas/farmacologia , Endo-1,4-beta-Xilanases/antagonistas & inibidores , Inibidores Enzimáticos/farmacologia , Polissacarídeos/metabolismo , Hidrólise/efeitos dos fármacos , Cinética
13.
Aging (Albany NY) ; 12(4): 3486-3501, 2020 02 09.
Artigo em Inglês | MEDLINE | ID: mdl-32039832

RESUMO

This work aimed to investigate tumor-infiltrating immune cells (TIICs) and immune-associated genes in the tumor microenvironment of osteosarcoma. An algorithm known as ESTIMATE was applied for immune score assessment, and osteosarcoma cases were assigned to the high and low immune score groups. Immune-associated genes between these groups were compared, and an optimal immune-related risk model was built by Cox regression analyses. The deconvolution algorithm (referred to as CIBERSORT) was applied to assess 22 TIICs for their amounts in the osteosarcoma microenvironment. Osteosarcoma cases with high immune score had significantly improved outcome (P<0.01). The proportions of naive B cells and M0 macrophages were significantly lower in high immune score tissues compared with the low immune score group (P<0.05), while the amounts of M1 macrophages, M2 macrophages, and resting dendritic cells were significantly higher (P<0.05). Important immune-associated genes were determined to generate a prognostic model by Cox regression analysis. Interestingly, cases with high risk score had poor outcome (P<0.01). The areas under the curve (AUC) for the risk model in predicting 1, 3 and 5-year survival were 0.634, 0.781, and 0.809, respectively. Gene set enrichment analysis suggested immunosuppression in high-risk osteosarcoma patients, in association with poor outcome.


Assuntos
Neoplasias Ósseas/patologia , Linfócitos do Interstício Tumoral/imunologia , Osteossarcoma/patologia , Microambiente Tumoral/imunologia , Algoritmos , Neoplasias Ósseas/imunologia , Neoplasias Ósseas/mortalidade , Bases de Dados Factuais , Perfilação da Expressão Gênica , Humanos , Linfócitos do Interstício Tumoral/patologia , Macrófagos/patologia , Osteossarcoma/imunologia , Osteossarcoma/mortalidade , Prognóstico , Taxa de Sobrevida
14.
Epidemiol Infect ; 148: e127, 2020 02 14.
Artigo em Inglês | MEDLINE | ID: mdl-32054550

RESUMO

Transmission of varicella occurs frequently in schools and households. We investigated the characteristics of varicella cases derived from within-household transmission and the modes of varicella transmission between school and household settings in Shanghai, China, from 2009 to 2018. Within-household transmission occurred in 278 households, of which 134 transmission events were between children. Sixty-one household varicella transmission events may be attributed to isolation procedures for infected students during school outbreaks, and 7.6% of school outbreaks were caused by schoolchildren cases derived from within-household transmission. The frequency of 'school-household-school' transmission adds an additional layer of complexity to the control of school varicella outbreaks. Administration of varicella vaccine as post-exposure prophylaxis after exposure is considered to be an effective measure to control varicella spread within households and schools.


Assuntos
Varicela/epidemiologia , Varicela/transmissão , Surtos de Doenças , Instituições Acadêmicas , Adolescente , Adulto , Criança , Pré-Escolar , China/epidemiologia , Características da Família , Feminino , Humanos , Lactente , Masculino , Adulto Jovem
15.
Talanta ; 209: 120611, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31892093

RESUMO

In this work, a novel "signal-on" electrochemical peptide biosensor based on peptide-conjugated hemin/G-quadruplex (DNAzyme-peptide) hybrid and rosebud-like MoSe2@reduced graphene oxide (MoSe2@rGO) nanocomposite, was developed for detection of prostate-specific antigen (PSA). Interestingly, the peptide not only served as recognition probe to detect PSA, but also acted as the enhancer to improve the enzyme activity of hemin/G4, which promoted the detection sensitivity. Up addition of PSA, Fe3O4-labeled DNAzyme-peptide probe was cleaved, followed by the magnetic separation. The cleaved DNAzyme-peptide was then captured onto the cysteine-modified electrode via the interaction between carboxyl groups of peptide and amino group of cysteine. A strong electrochemical signal was obtained from hemin and further was amplified by the enhanced electrocatalysis of DNAzyme-peptide. Compared to the original DNAzyme, DNAzyme-peptide exhibited more than 3-fold enhancement in signal amplification. And MoSe2@rGO amplified the electrochemical signal due to its good conductivity and large surface area. So the proposed strategy detected PSA down to 0.3 fg/mL, and it showed the advantages of simplicity, low cost by avoiding the use of expensive protein enzyme and additional electroactive species. Therefore, the proposed biosensor potentially provided a very effective tool for early diagnosis of cancer by the detection of PSA.


Assuntos
Técnicas Biossensoriais/métodos , Quadruplex G , Hemina/química , Peptídeos/química , Antígeno Prostático Específico/sangue , DNA Catalítico/química , Técnicas Eletroquímicas/métodos , Grafite/química , Humanos , Limite de Detecção , Nanocompostos/química , Nanocompostos/ultraestrutura , Neoplasias/sangue
16.
Cancer Cell Int ; 19: 311, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31768131

RESUMO

Background: Clear cell renal cell carcinoma (ccRCC) is the most common renal cell carcinoma subtype with a poor prognosis. LncRNA-LET is a long non-coding RNA (lncRNA) that is down-regulated in ccRCC tissues. However, its role in ccRCC development and progress is unclear. Methods: LncRNA-LET expression was detected in ccRCC tissues and ccRCC cells using quantitative real-time PCR. The overexpression and knockdown experiments were performed in ccRCC cells and xenograft mouse model to evaluate role of lncRNA-LET. Cell cycle, apoptosis and JC-1 assays were conducted via flow cytometer. The protein levels were measured through western blot analysis and the interaction between lncRNA-LET and miR-373-3p was identified via luciferase reporter assay. Results: LncRNA-LET expression was lower in ccRCC tissues than that in the matched adjacent non-tumor tissues (n = 16). In vitro, lncRNA-LET overexpression induced cell cycle arrest, promoted apoptosis and impaired mitochondrial membrane potential, whereas its knockdown exerted opposite effects. Moreover, we noted that lncRNA-LET may act as a target for oncomiR miR-373-3p. In contrast to lncRNA-LET, miR-373-3p expression was higher in ccRCC tissues. The binding between lncRNA-LET and miR-373-3p was validated. Two downstream targets of miR-373-3p, Dickkopf-1 (DKK1) and tissue inhibitor of metalloproteinase-2 (TIMP2), were positively regulated by lncRNA-LET in ccRCC cells. MiR-373-3p mimics reduced lncRNA-LET-induced up-regulation of DKK1 and TIMP2 levels, and attenuated lncRNA-LET-mediated anti-tumor effects in ccRCC cells. In vivo, lncRNA-LET suppressed the growth of ccRCC xenograft tumors. Conclusion: These findings indicate that lncRNA-LET plays a tumor suppressive role in ccRCC by regulating miR-373-3p.

17.
J Phys Condens Matter ; 31(33): 335601, 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31067512

RESUMO

We propose an approach that is under the framework of Gutzwiller wave function but goes beyond the commonly adopted Gutzwiller approximation to improve the accuracy and flexibility in treating the correlation effects. Detailed formalism is described for a dimer which is straightforwardly generalized later to more complicated periodic bulk systems. The accuracy of the approach is demonstrated by evaluating the potential energy curves of spin-singlet N2 dimer, spin-triplet O2 dimer, and 1D hydrogen chain. The computational workload of the approach can be easily handled by efficient parallel computing.

18.
Zhongguo Zhong Yao Za Zhi ; 44(3): 482-488, 2019 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-30989912

RESUMO

The powder X-ray diffraction(PXRD) technique was used to investigate fourteen kinds of Ranunculaceae herbal decoction pieces(RHDP) recorded in Chinese Pharmacopoeia and to explore a novel PXRD quality control method for RHDP. The results indicated that only three RHDP-Paeoniae Radix Alba, Paeoniae Radix Rubra, and Moutan Cortex, contained calcium oxalate monodydrate(COM), whereas no COM existed in other eleven kinds of RHDP. The difference in PXRD for Paeoniae Radix Alba and Paeoniae Radix Rubra from different growing areas were investigated. The quantitative analysis method for COM was discussed by considering the water-boiling manufacturing process of herbal decoction pieces. The water-boiling experiments revealed that the PXRD peaks from COM crystals in RHDP were enhanced significantly after boiling. Paeoniae Radix Alba, Paeoniae Radix Rubra, Moutan Cortex, Aconiti Lateralis Radix Praeparata, Aconiti Radix, Aconiti Kusnezoffii Radix, and Anemone Raddeanae Rhizoma exhibited a similar series of broader peaks in the 2θ region of 15° to 35°, whose origins were discussed on the basis of chemical constituents RHDP reported by other researchers. These diffraction broader peaks most likely originated from periodic orientation of benzene ring in organic molecular crystals of aconitine-and paeonolum-based alkaloids and glycosides chemical constituents, subsequently, possibly from some other organic constituents. The PXRD technique can be used to rapidly identify Cimicifuga heracleifolia with an amorphous dispersion peak and C. dahurica with a sharp-peak feature. Climatidis Radix et Rhizoma exhibited a series of sharp PXRD peaks. The PXRD method can provide a valuable quality control method for RHDP.


Assuntos
Medicamentos de Ervas Chinesas/química , Compostos Fitoquímicos/análise , Ranunculaceae/química , Aconitum/química , Paeonia/química , Rizoma/química , Difração de Raios X
19.
Analyst ; 144(6): 2120-2129, 2019 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-30741272

RESUMO

A novel type of sandwich MOF was successfully synthesized on reduced graphene oxide (denoted as M@Pt@M-rGO) by an in situ synthesis method. The obtained M@Pt@M-rGO possesses excellent electrochemical properties. The surface morphology and structure of M@Pt@M-rGO were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), etc. By using M@Pt@M-rGO, a novel electrochemical sensor was constructed and successfully used for the simultaneous and sensitive detection of three isomers: hydroquinone (HQ), catechol (CT) and resorcinol (RS), with wider linear ranges of concentrations of 0.05-200 µM, 0.1-160 µM and 0.4-300 µM and lower detection limits of 0.015 µM, 0.032 µM and 0.133 µM (S/N = 3) for HQ, CT and RS, respectively. Besides, the proposed electrochemical sensor showed excellent anti-interference capability, high stability, good reproducibility, and satisfactory recovery for determination of isomers in river and lake water.

20.
Oncol Res ; 27(9): 1007-1014, 2019 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-29673422

RESUMO

Renal carcinoma greatly threatens human health, but the involved molecular mechanisms are far from complete understanding. As a master oncogene driving the initiation of many other cancers, ZBTB7 has not been established to be associated with renal cancer. Our data revealed that ZBTB7 is highly expressed in renal carcinoma specimens and cell lines, compared with normal cells. The silencing of ZBTB7 suppressed the proliferation and invasion of renal cancer cells. ZBTB7 overexpression rendered normal cells with higher proliferation rates and invasiveness. An animal study further confirmed the role of ZBTB7 in the growth of renal carcinoma. Moreover, miR-137 was identified to negatively regulate the expression of ZBTB7, and its abundance is inversely correlated with that of ZBTB7 in renal carcinoma specimens and cell lines. ZBTB7 overexpression may be induced by miR-137 downregulation. Interestingly, ZBTB7 can also suppress miR-137 expression by binding to its recognition site within the miR-137 promoter region. Taken together, we identified an autoregulatory loop consisting of ZBTB7 and miR-137 in gastric cancers, and targeting this pathway may be an effective strategy for renal carcinoma cancer therapy.


Assuntos
Proteínas de Ligação a DNA/metabolismo , Neoplasias Renais/metabolismo , MicroRNAs/metabolismo , Fatores de Transcrição/metabolismo , Animais , Linhagem Celular Tumoral , Proliferação de Células/fisiologia , Proteínas de Ligação a DNA/biossíntese , Proteínas de Ligação a DNA/genética , Progressão da Doença , Regulação para Baixo , Xenoenxertos , Humanos , Neoplasias Renais/genética , Neoplasias Renais/patologia , Camundongos , MicroRNAs/genética , Invasividade Neoplásica , Regiões Promotoras Genéticas , Fatores de Transcrição/biossíntese , Fatores de Transcrição/genética , Transcrição Genética
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