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Nanoscale ; 13(45): 19085-19097, 2021 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-34761764


Polypyrrole (PPy) nanoparticles have been widely studied in tumor photothermal therapy (PTT) for their significant photostability, good biocompatibility, and excellent photothermal performance. Herein, we report bovine serum albumin (BSA) stabilized PPy that were mineralized by MnO2 nanozyme on the surface (PPy@BSA-MnO2) to achieve synergistic photothermal and chemodynamic therapy (CDT) for breast cancer. In this multifunctional nanoplatform, the surface-loaded MnO2 undergoes a redox reaction with glutathione (GSH) to generate glutathione disulfide (GSSG) and Mn2+. Then, Mn2+ can convert H2O2 into a highly cytotoxic ˙OH to achieve chemodynamic therapy (CDT) and possess good magnetic resonance (MR) T1-weighted imaging capabilities to realize contrast imaging of the 4T1 tumor-bearing mouse models. In addition, PPy nanoparticles can efficiently convert near-infrared light energy into heat and achieve PTT. Most importantly, PPy@BSA-MnO2 nanoprobes have excellent in vitro 4T1 cell-killing effect and in vivo tumor-suppressive properties. The acute toxicity assessment results indicate that PPy@BSA-MnO2 nanoprobes have good biological safety. Therefore, the as-prepared multifunctional PPy@BSA-MnO2 nanoprobes possess excellent performance to promote MRI-guided PTT/CDT synergistic therapy for breast cancer treatment and have extensive clinical transformation and application prospects.

Neoplasias , Polímeros , Animais , Peróxido de Hidrogênio , Imageamento por Ressonância Magnética , Compostos de Manganês , Camundongos , Óxidos , Pirróis , Nanomedicina Teranóstica
Anal Bioanal Chem ; 413(20): 5239-5249, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34212211


A sensitive and effective strategy for the detection of cytochrome c (Cyt c) and trypsin was developed using biomass nitrogen-doped carbon quantum dots (N-CQDs) as the fluorescence probe. N-CQDs were synthesized through a one-pot hydrothermal method by utilizing cellulolytic enzyme lignin as the carbon source and ammonia as the solvent and nitrogen source. The obtained N-CQDs had good water solubility and stable optical properties. The introduction of nitrogen increased fluorescence quantum yield (QY) to 8.23%, which was almost four times as high as that before nitrogen doping. The N-CQDs were fabricated as a label-free biosensor to detect Cyt c and trypsin. The fluorescence of N-CQDs was quenched with positively charged Cyt c due to electrostatic induction aggregation and static quenching. However, Cyt c tended to be hydrolyzed into small peptides in the presence of trypsin, which caused fluorescence recovery of the N-CQDs/Cyt c complex. A wide linear response range was achieved for Cyt c within 1-50 µM and the developed N-CQDs/Cyt c complex displayed a linear response for trypsin within 0.09-5.4 U/mL. The detection limits were 0.29 µM for Cyt c and 0.013 U/mL for trypsin, respectively. Furthermore, this assay had been applied to Cyt c and trypsin detection in serum samples with the recoveries in the range of 94.6-98.5% and 95.5-102.0%, respectively. The established method was sensitive, selective, easy to operate, and low cost, which proved its potential application in clinical diagnosis. The synthesis and fluorescence mechanism of N-CQDs and the strategy for Cyt c and trypsin detection.

Carbono/química , Citocromos c/química , Nitrogênio/química , Tripsina/química , Citocromos c/sangue , Citocromos c/metabolismo , Humanos , Lignina/química , Lignina/metabolismo , Estrutura Molecular , Pontos Quânticos , Sensibilidade e Especificidade , Soro , Espectrometria de Fluorescência , Tripsina/sangue , Tripsina/metabolismo
Sci Rep ; 8(1): 12445, 2018 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-30127385


Graphene has been widely utilized in optoelectronic applications due to its high carrier mobility, and extremely fast optical response. Microcavity-integrated graphene waveguide structure is one basic module of integrated photonic devices which can greatly improve the light-matter interaction strength. The enhanced optical absorption in the undoped graphene layer results from the light trapping and the corresponding long light-graphene interaction length. Tuning the Fermi energy level of the graphene layer enables the electro-optical modulation. We report the realization of reconfigurable electro-optical attenuator and switch with unity-order modulation depth in light reflection and transmission at near-infrared frequency. The transformation from a lossy absorber to a quasi-perfect transparent condition of the monolayer graphene by tuning the Fermi level leads to the unity-order tunability of the electro-optical attenuator and switch. We investigate theoretically and numerically the absorption properties of the designed microcavity-integrated graphene with respect to different graphene Fermi levels. Electro-optical attenuator with attenuating coefficient from 10% to 98.29% is fulfilled. On-off electro-optical switching with a switching contrast larger than 21 dB is demonstrated. Our approach provides the possibilities of graphene photonics applied in communications, and sensing.