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1.
Zhongguo Zhong Yao Za Zhi ; 46(6): 1393-1400, 2021 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-33787137

RESUMO

L~*, a~* and b~* values of prepared slices of Curcumae Rhizoma were measured by spectrophotometer. SPSS 21.0 was used for discriminant analysis to establish the color range and mathematical prediction model of prepared slices of Curcumae Rhizoma. The values of L~*, a~* and b~* of kwangsiensis ranged from 58.09-62.40, 4.53-5.66 and 23.61-24.29, while the values of L~*, a~* and b~* of phaeocaulis were between 64.02-70.71,-0.89-4.13 and 44.59-54.52, respectively. The values of L~*, a~* and b~* of wenyujin were 68.55-70.99,-0.11-1.47 and 28.26-32.19, respectively. The mathematical prediction model was proved to be able to realize 100% identification of Curcumae Rhizome of different origins through original and cross validation and external samples validation. A dual wavelength HPLC was established; the contents of 9 sesquiterpenoids and 3 Curcumae Rhizomes were determined simultaneously; and the contents of Curcumae Rhizome of different origins were determined. The results showed that kwangsiensis had higher contents of neocurdione, ß-elemene and isocurcumaenol, phaeocaulis curcumin, furadienone, demethoxycurcumin and curcumin; and wenyujin mainly contained curdione, furadienes and guimarone. Pearson correlation analysis on L~*, a~*, b~* value and content of 12 components showed that curcumin, furadienone, demethoxycurcumin and curcumin had a significant positive correlation with b~* value(P<0.01). There was a significant negative correlation between neocurdione, ß-elemene and isocurcumaenol and L~* value(P<0.01). Curdione, furadienes and guimarone were significantly correlated with L~* value(P<0.01),indicating that the appearance co-lor of Curcumae Rhizoma could reflect the change of the content of the internal components. This study provided reference for the rapid recognition of Curcumae Rhizoma and the establishment of quality evaluation system.


Assuntos
Curcumina , Rizoma , Cromatografia Líquida de Alta Pressão , Cor , Curcuma
2.
Nat Prod Bioprospect ; 11(1): 73-79, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33083968

RESUMO

A phytochemical investigation of the EtOH extract of the flowers of Lagerstroemia indica L. led to the isolation and characterization of a new pyrrole alkaloid, named lagerindicine (1), along with four known compounds (2-5). Their structures were elucidated by the detailed spectroscopic analysis and comparison with literature data, whereas the structure, in particularly, the absolute configuration (AC) of 1, was firmly determined by total synthesis. All the isolates were evaluated for their cytotoxic effects against human colon cancer cell (HCT-116), and compound 3 exhibited weak cytotoxicity with IC50 value of 28.4 µM.

3.
Steroids ; 155: 108558, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-31866544

RESUMO

Two new hydroperoxy steroids, namely, xidaosteroids A and B (1 and 2), along with five known related compounds 3-7, were isolated from the South China Sea gorgonian Rumphella sp.. Their structures were established by extensive spectroscopic analyses and comparison of the spectral data with those reported in the literature. In bioassay, compound 3 showed weak cytotoxicities against SNU-398 and Capan-1 cells.


Assuntos
Antozoários/química , Antineoplásicos/farmacologia , Células A549 , Animais , Antineoplásicos/química , Antineoplásicos/isolamento & purificação , Proliferação de Células/efeitos dos fármacos , China , Ensaios de Seleção de Medicamentos Antitumorais , Células HT29 , Humanos , Conformação Molecular , Estereoisomerismo
4.
Zhongguo Zhong Yao Za Zhi ; 43(18): 3795-3800, 2018 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-30384548

RESUMO

Chinese medicine processing is the main feature that distinguishes traditional Chinese medicine from natural medicine and plant medicine, and is the main feature in clinical medication of traditional Chinese medicine. The research of Chinese medicine processing technology is an important link to realize standardization and standardization of Chinese herbal pieces, with urgent need to attract high attention. At present, there are still many problems in the research of processing technology of Chinese herbal pieces, mainly including inconsistent processing technology, large differences in process technology parameters, and unstable production technology of Chinese herbal pieces, resulting in uncontrollable quality of Chinese herbal pieces and affecting the clinical efficacy of Chinese medicine. This paper focused on the establishment of a unified standard processing technology, and put forward the countermeasures for the processing technology of Chinese medicine based on a comprehensive analysis of the current situations of the processing technology of Chinese herbal pieces, with significance for guiding the establishment of a standardized processing technology of Chinese medicine.


Assuntos
Medicamentos de Ervas Chinesas/química , Medicina Tradicional Chinesa , Tecnologia Farmacêutica/métodos , Controle de Qualidade , Padrões de Referência , Pesquisa
5.
Mol Cell Endocrinol ; 417: 103-13, 2015 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-26419930

RESUMO

Estrogen replacement therapy (ERT) is utilized as a major regime for treatment of postmenopausal osteoporosis at present. However, long-term supplement of estrogen may cause uterine hyperplasia and hypertension leading to a high risk of endometrial cancer and breast cancer. Psoralea corylifolia L. has long been used as tonic and food additives in many countries. Previous studies had found two ingredients in P. corylifolia L.: bavachin and bakuchiol exhibited osteoblastic activity. The present study was designed to investigate the protective effect of bakuchiol and bavachin on ovariectomy-induced bone loss and explore the possible mechanism. In vivo, bakuchiol and bavachin could prevented estrogen deficiency-induced bone loss in ovariectomized rats without uterotrophic activity. In vitro studies suggested that bakuchiol and bavachin induced primary human osteoblast differentiation by up-regulating the Wnt signalling pathway. This study suggests that such a bone-protective role makes them a promising and safe estrogen supplement for the ERT.


Assuntos
Flavonoides/administração & dosagem , Osteoporose/tratamento farmacológico , Ovariectomia/efeitos adversos , Fenóis/administração & dosagem , Psoralea/química , Animais , Diferenciação Celular/efeitos dos fármacos , Células Cultivadas , Modelos Animais de Doenças , Feminino , Flavonoides/farmacologia , Humanos , Osteoblastos/citologia , Osteoblastos/efeitos dos fármacos , Osteoclastos/efeitos dos fármacos , Osteoporose/etiologia , Fenóis/farmacologia , Extratos Vegetais/administração & dosagem , Extratos Vegetais/farmacologia , Ratos , Regulação para Cima , Via de Sinalização Wnt/efeitos dos fármacos
6.
Zhong Yao Cai ; 38(6): 1168-71, 2015 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-26762055

RESUMO

OBJECTIVE: To improve the traditional fingerprint method to distinguish vinegar processed Genkwa Flos from raw Genkwa Flos. METHODS: Ten batches of Genkwa Flos were collected, processed with vinegar through a standard method, and then analyzed under the optimum HPLC condition. Based on the chromatographic data obtained, a common model of vinegar processed Genkwa Flos fingerprints, including 11 common peaks and the components genkwanin, hydroxygenkwanin, luteolin, apigenin and yuanhuacin were identified, was established. The peak of baicalein, an exogenous component added quantitatively to the samples as an internal standard, was served as the reference peak. The similarity between the test samples and the common model was computed using the improved Euclidean distance method developed in this paper. RESULTS: The similarities between vinegar processed Genkwa Flos samples and the common model were higher than 0.9, whereas those between raw Genkwa Flos and the common model were lower than 0.9. CONCLUSION: The proposed method thus effectively provides a clear distinction between vinegar processed and raw Genkwa Flos samples. The result is helpful to ensure the safe clinical use of the plant and expand the application field of fingerprinting technology.


Assuntos
Ácido Acético , Daphne/química , Medicamentos de Ervas Chinesas/química , Flores/química , Apigenina , Cromatografia Líquida de Alta Pressão , Diterpenos , Flavonas , Flavonoides , Luteolina
7.
Zhongguo Zhong Yao Za Zhi ; 39(15): 2791-5, 2014 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-25423810

RESUMO

Infestation, moldy and other phenomenon in the processing and storage of Chinese herbal medicines is a problem that faced in the production of Chinese traditional medicine. The low productivity of traditional processing methods can not guarantee the quality of Chinese herbal medicines. Sulfur fumigation is the first choice of grassroots to process the Chinese herbal medicine with its low cost and easy operation. Sulfur fumigation can solve some problems in the processing and storage of Chinese herbal medicines, but modern pharmacological studies show that long-term use of Chinese traditional medicine which is fumigated by sulfur can cause some serious harm to human liver, kidney and other organs. This paper conducts a review about the application history of sulfur fumigation, its influence to the quality of Chinese herbal medicines as well as domestic and foreign limits to sulfur quantity, and a brief introduction of the status of modern processing technologies in the processing of food and some Chinese herbal medicines, the problems ex- isting in the Chinese herbal medicines processing, which can provide a reference basis for the further research, development and application of investigating alternative technologies of sulfur fumigation.


Assuntos
Fumigação/métodos , Medicina Tradicional Chinesa/métodos , Enxofre/química , Tecnologia Farmacêutica/métodos , Fumigação/efeitos adversos , Controle de Qualidade , Controle Social Formal
8.
Zhongguo Zhong Yao Za Zhi ; 39(15): 2796-800, 2014 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-25423811

RESUMO

Sulfur fumigation (SF) is a universal phenomenon in primary processing of Traditional Chinese Medicine (TCM) in modern times. In the process, fumigation, sulfur or both of them act on the TCMs. Some active components of TCMs change quantitatively or qualitatively during the processing. At the same time, the sulfur dioxide and heavy metal would remain and cause a serious influence on quality and future development of TCM. This article reviews the chemical compositions change after SF to study the change law and their influence on quality. This article provide references for SF in TCMs' processing for a better and safer quality.


Assuntos
Fumigação/métodos , Medicina Tradicional Chinesa/métodos , Enxofre/química , Tecnologia Farmacêutica/métodos , Contaminação de Medicamentos , Controle de Qualidade
9.
Zhongguo Zhong Yao Za Zhi ; 39(1): 149-52, 2014 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-24754185

RESUMO

Traditional Chinese medicine (TCM) reference standards plays an important role in the quality control of Chinese herbal pieces. This paper overviewed the development of TCM reference standards. By analyzing the 2010 edition of Chinese pharmacopoeia, the application of TCM reference standards in the quality control of Chinese herbal pieces was summarized, and the problems exiting in the system were put forward. In the process of improving the quality control level of Chinese herbal pieces, various kinds of advanced methods and technology should be used to research the characteristic reference standards of Chinese herbal pieces, more and more reasonable reference standards should be introduced in the quality control system of Chinese herbal pieces. This article discussed the solutions in the aspect of TCM reference standards, and future development of quality control on Chinese herbal pieces is prospected.


Assuntos
Medicamentos de Ervas Chinesas/normas , Medicina Tradicional Chinesa/normas , Humanos , Controle de Qualidade , Padrões de Referência
10.
Zhongguo Zhong Yao Za Zhi ; 39(19): 3701-6, 2014 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-25612424

RESUMO

As a kind of commonly used traditional Chinese medicine, ginseng has a high reputation at home and abroad. The research of ginseng has been expanded to medicine, pharmacy, biology, food science and other fields, with great achievements in recent years. Ginseng contains ginsenosides, volatile oil, carbohydrates, amino acids, polypeptides, inorganic elements and othser chemical constituents. Each component has extensive physiological activity, and is the base of ginseng's effect. After processing, the complicated changes are taken place in the constituents of ginseng, and some new substances produced. This paper aims to review the studies on chemical constituents and their mechanisms during ginseng processing, and the ideas, methods and the direction of the development of traditional Chinese medicine processing in the future.


Assuntos
Química Farmacêutica/métodos , Medicamentos de Ervas Chinesas/química , Panax/química , Plantas Medicinais/química
11.
Guang Pu Xue Yu Guang Pu Fen Xi ; 33(9): 2315-20, 2013 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-24369622

RESUMO

The paper reviewed the research progress in the spectral color science, including the common model, color measurement instrument in recent years, application of color measurement technology in agriculture, food, industry, medicine and other fields. The possibility to achieve the color quantization of Chinese medicine was explored by color measurement technology, and analyzed its application prospect in the field of Chinese medicine, provided a model for the study on quality control for Chinese medicine non-destructive online


Assuntos
Cor , Medicina Tradicional Chinesa , Análise Espectral , Modelos Teóricos , Controle de Qualidade
12.
Zhongguo Zhong Yao Za Zhi ; 38(3): 346-9, 2013 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-23668006

RESUMO

OBJECTIVE: To optimize the preparation of salt-processed Psoraleae Fructus by the orthogonal test method. METHOD: With the 12 components such as coumarin, flavone and phenolics as study indexes, a L9 (3(4)) orthogonal test was adopted to compare the effect of different factors on salt-processed Psoraleae Fructus. RESULT: According to the finalized optimal process, 100 g Psoraleae Fructus was added with 2 g salt, moistened for 2 hours, and then fried for 10 min at 150 degrees. CONCLUSION: The orthogonal test is so reasonable and reliable that it can provide basis for the preparation of salt-processed Psoraleae Fructus.


Assuntos
Medicamentos de Ervas Chinesas/química , Frutas/química , Psoralea/química , Cloreto de Sódio/química , Tecnologia Farmacêutica/métodos , Cromatografia Líquida de Alta Pressão , Cumarínicos/análise , Flavonas/análise , Temperatura Alta , Fenóis/análise , Reprodutibilidade dos Testes
13.
Zhong Yao Cai ; 34(11): 1728-9, 2011 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-22506399

RESUMO

OBJECTIVE: To study the chemical constituents of vinegar Schisandra chinensis. METHODS: Column chromatography was used in the isolation procedure. The structures of isolated compounds were elucidated by spectral data. RESULTS: Five compounds were isolated and their structures were identified as kadsuranin (I), beta-sitosterol (II), schizandrin (III), 5,5'-[oxybis (methylene)] bis-2-furancarboxaldehyde (IV) and (NH)-form-2-Hydroxyquinoline4-carboxylic acid (V). CONCLUSION: Compound 5 is isolated from this genus for the first time.


Assuntos
Ácido Acético , Ciclo-Octanos/isolamento & purificação , Lignanas/isolamento & purificação , Plantas Medicinais/química , Compostos Policíclicos/isolamento & purificação , Schisandra/química , Tecnologia Farmacêutica/métodos , Ciclo-Octanos/química , Frutas/química , Lignanas/química , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Compostos Policíclicos/química , Sitosteroides/química , Sitosteroides/isolamento & purificação
14.
Int J Biochem Cell Biol ; 40(5): 942-53, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18155951

RESUMO

We have previously implicated reactive oxygen species oxygen (ROS) as a critical signal transducer in the upregulation of Na,K-ATPase by low K+ in MDCK cells, but how ROS mediate this process has not been well defined. We reported here that both of hydrogen peroxide (H2O2) and superoxide anion (O2*(-)) were rapidly produced at the early stage of low K+-treated MDCK cells. Further analysis revealed that NADP/NADPH oxidase-derived H2O2 was specifically involved in low K+-induced Na,K-ATPase alpha1 gene transcription as well as alpha1 and beta1 subunits expressions. Exogenous H2O2 even mimicked the stimulatory effect of low K+ on Na,K-ATPase alpha1 gene transcription. Low K+ triggered a H2O2-dependent ERK1/2 phosphorylation in MDCK cells, nonetheless, this ERK1/2 activation did not finally lead to the upregulation of Na,K-ATPase. Similar to previous findings that Na,K-ATPase beta1 gene transcription was mediated by Sp1, Na,K-ATPase alpha1 gene transcription in low K+-treated MDCK cells was also closely relevant to Sp1 participation, as confirmed by siRNA as well as PCR mutagenesis technologies. Furthermore, Sp1 activation was dependent on H2O2 generation triggered by low K+. Taken together, the data described in this study outlines an essential role of H2O2 and Sp1 in mediating the upregulation of Na,K-ATPase in MDCK cells by low external K+.


Assuntos
Células Epiteliais/metabolismo , Peróxido de Hidrogênio/metabolismo , ATPase Trocadora de Sódio-Potássio/genética , Fator de Transcrição Sp1/metabolismo , Animais , Catalase/metabolismo , Linhagem Celular , Cães , Células Epiteliais/efeitos dos fármacos , Células Epiteliais/enzimologia , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Regulação Enzimológica da Expressão Gênica , NADPH Oxidases/metabolismo , Oxirredução , Potássio/farmacologia , ATPase Trocadora de Sódio-Potássio/metabolismo , Superóxido Dismutase/metabolismo , Superóxidos/metabolismo , Transcrição Genética , Regulação para Cima
15.
Food Chem Toxicol ; 45(9): 1700-8, 2007 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-17449162

RESUMO

To study the cytotoxicity of four alkaloids: brucine, strychnine, brucine N-oxide and isostrychnine from nux vomica on SMMC 7721 cells and their possible mechanisms, MET assay was used to examine the growth inhibitory effects of these alkaloids. Brucine revealed the strongest growth inhibitory effect on SMMC-7721 cells. Furthermore, as directly observed under an inverted microscope, fluorescent microscope and transmission electronic microscope, brucine caused SMMC-7721 cell shrinkage, membrane blobbing, formation of apoptotic body as well as nucleus condensation, all of which are typical characteristics of apoptotic programmed cell death. In addition, brucine dose-dependently caused SMMC-7721 cells apoptosis via formation of subdipolid DNA and phosphatidylserine externalization, as evidenced by flow cytometry analysis. The brucine-induced apoptosis was partially attributed to the activation of caspase 3 as well as cyclooxygenase 2 inhibition, since neither caspase 3 specific inhibitor, z-DEVD-fmk nor was exogenous addition of prostaglandin E(2) able to completely abrogate the brucine-induced SMMC 7721 cell apoptosis. In sum, this paper indicate that the major alkaloids present in the seed of Strychnos nux-vomica are effective against SMMC-7721 cells proliferation, among which brucine proceeds SMMC-7721 cells death via apoptosis, probably through the participation of caspase 3 and cyclooxygenase 2.


Assuntos
Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Caspase 3/metabolismo , Ciclo-Oxigenase 2/metabolismo , Estricnina/análogos & derivados , Strychnos nux-vomica , Carcinoma Hepatocelular/tratamento farmacológico , Carcinoma Hepatocelular/enzimologia , Caspase 3/biossíntese , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Óxidos N-Cíclicos/toxicidade , Relação Dose-Resposta a Droga , Ativação Enzimática , Citometria de Fluxo , Humanos , Neoplasias Hepáticas/tratamento farmacológico , Neoplasias Hepáticas/enzimologia , Sementes/química , Estricnina/farmacologia , Estricnina/toxicidade , Strychnos nux-vomica/química
16.
J Ethnopharmacol ; 106(2): 179-86, 2006 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-16442763

RESUMO

To screen the anti-tumor effects of the four alkaloids: brucine, strychnine, brucine N-oxide and isostrychnine from the seed of Strychnos nux-vomica, MTT assay was used to examine the growth inhibitory effects of these alkaloids on human hepatoma cell line (HepG2). Brucine, strychnine and isostrychnine revealed significant inhibitory effects against HepG2 cell proliferation, whereas brucine N-oxide didn't have such an effect. In addition, brucine caused HepG2 cell shrinkage, membrane blebbing, apoptotic body formation, all of which are typical characteristics of apoptotic programmed cell death. The results of flow cytometric analysis demonstrated that brucine caused dose-dependent apoptosis of HepG2 cells through cell cycle arrest at G0/G1 phase, thus preventing cells entering S or G2/M phase. Immunoblot results revealed that brucine significantly decreased the protein expression level of cyclooxygenase-2, whereas increased the expression caspase-3 as well as the caspase-3-like protease activity in HepG2 cells, suggesting the involvement of cyclooxygenase-2 and caspase-3 in the pro-apoptotic effects exerted by brucine. Therefore, this paper indicate that the major alkaloids present in the seed of Strychnos nux-vomica are effective against HepG2 cells proliferation, among which brucine proceed HepG2 cells death via apoptosis, probably through the participation of caspase-3 and cyclooxygenase-2.


Assuntos
Alcaloides/farmacologia , Antineoplásicos/farmacologia , Carcinoma Hepatocelular/tratamento farmacológico , Neoplasias Hepáticas/tratamento farmacológico , Strychnos nux-vomica/química , Alcaloides/isolamento & purificação , Alcaloides/uso terapêutico , Antineoplásicos/isolamento & purificação , Antineoplásicos/uso terapêutico , Apoptose/efeitos dos fármacos , Carcinoma Hepatocelular/enzimologia , Carcinoma Hepatocelular/patologia , Caspase 3/biossíntese , Ciclo Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Forma Celular/efeitos dos fármacos , Óxidos N-Cíclicos/isolamento & purificação , Óxidos N-Cíclicos/farmacologia , Ciclo-Oxigenase 2/metabolismo , Relação Dose-Resposta a Droga , Humanos , Neoplasias Hepáticas/enzimologia , Neoplasias Hepáticas/patologia , Sementes , Estricnina/análogos & derivados , Estricnina/isolamento & purificação , Estricnina/farmacologia
17.
J Ethnopharmacol ; 88(2-3): 205-14, 2003 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-12963144

RESUMO

To further understand the purpose of the traditional processing method of the seeds of Strychnos nux-vomica L. (Loganiaceae) as well as analgesic and anti-inflammatory activities of brucine and brucine N-oxide extracted from this medicinal plant, various pain and inflammatory models were employed in the present study to investigate their pharmacological profiles. Both brucine and brucine N-oxide revealed significant protective effects against thermic and chemical stimuli in hot-plate test and writhing test. However, on different phases they exerted analgesic activities in formalin test. Brucine N-oxide showed stronger inhibitory effect than brucine in carrageenan-induced rat paw edema, both of them significantly inhibited the release of prostaglandin E2 in inflammatory tissue, reduced acetic acid-induced vascular permeability and the content of 6-keto-PGF1a in Freund's complete adjuvant (FCA) induced arthritis rat's blood plasma. In addition, brucine and brucine N-oxide were shown to reduce the content of 5-hydroxytryptamine (5-HT) in FCA-induced arthritis rat's blood plasma, while increase the content of 5-hydroxytryindole-3-acetic acid (5-HIAA) accordingly. These results suggest that central and peripheral mechanism are involved in the pain modulation and anti-inflammation effects of brucine and brucine N-oxide, biochemical mechanisms of brucine and brucine N-oxide are different even though they are similar in chemical structure.


Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Óxidos N-Cíclicos/farmacologia , Estricnina/análogos & derivados , Estricnina/farmacologia , Strychnos nux-vomica/química , Animais , Anti-Inflamatórios não Esteroides/isolamento & purificação , Artrite Experimental/sangue , Artrite Experimental/induzido quimicamente , Artrite Experimental/tratamento farmacológico , Permeabilidade Capilar/efeitos dos fármacos , Óxidos N-Cíclicos/isolamento & purificação , Modelos Animais de Doenças , Edema/induzido quimicamente , Edema/tratamento farmacológico , Feminino , Masculino , Camundongos , Camundongos Endogâmicos ICR , Dor/induzido quimicamente , Dor/tratamento farmacológico , Extratos Vegetais/farmacologia , Ratos , Ratos Sprague-Dawley , Sementes/química , Serotonina/sangue , Estricnina/isolamento & purificação
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